A mouse heart radioablation mold based on 3D printing and flip-mold process

Abstract

The utility model discloses a mouse heart radioactivity damage mould based on 3D printing and mould turning process, including frame body structure, the whole is hollow cuboid structure without top, the frame body structure is spliced by left and right two connecting pieces, and this kind of structure is convenient for early processing and later storage, support piece is provided with two rows, and two rows of support pieces are symmetrically placed in the two side wall positions of frame body structure, the utility model discloses through 3D modeling calculation radio source under the lead plate barrier, the real data of accurate irradiation mouse heart, the lead plate mould is made based on three -dimensional structure data 3D printing, and accurate mould turning is carried out to low melting point lead through mould turning technology, realize the production of high accuracy, and X ray can accurately penetrate the radiation hole and form the circular irradiation field of 1cm diameter, realize the accurate radiotherapy of heart, and the radiation dose that other parts bear under the protection of 3cm thick lead plate is lower, and the animal mortality is low, and it is closer to the clinical practice.

Description

Technical Field

[0001] This invention belongs to the field of animal experimental technology, specifically relating to a mouse heart radiation injury mold based on 3D printing and molding technology. Background Technology

[0002] RIHD, short for Radiation-Induced Heat Damage, is a collective term for a range of heart diseases, including pericarditis, cardiomyopathy, coronary artery disease, valvular heart disease, and conduction system abnormalities. Because it is closely related to radiation, it is collectively referred to as RIHD. The mechanisms of RIHD are complex, involving multiple pathophysiological changes that interact, including endothelial cell damage, oxidative stress, DNA damage, and endoplasmic reticulum and mitochondrial abnormalities. These lead to microvascular damage and interstitial necrosis, resulting in myocardial ischemia and hypoxia, ultimately leading to cell necrosis and fibrosis. The clinical manifestations of RIHD are diverse, including pericarditis, myocardial injury, coronary artery disease, valvular heart disease, and arrhythmias. These complications can exist independently or occur in combination and continue to progress. However, the specific pathogenesis of RIHD is not yet fully understood, and related clinical studies are limited and often lack depth, with basic research being even scarcer. Therefore, conducting extensive basic research on RIHD-related animal models to elucidate its underlying molecular biological mechanisms is a crucial issue that urgently needs to be addressed.

[0003] Currently, existing animal RHID modeling devices, such as the device for animal heart radiation modeling disclosed in announcement number CN220274624U, cannot accurately calculate the true data of the radiation source passing through the radiation hole of the lead plate, resulting in insufficient accuracy of radiation to the heart; in addition, the thickness of the lead plate is not sufficient to effectively prevent X-ray damage to other parts; at the same time, the number of animals that can be modeled at one time is also relatively limited. Utility Model Content

[0004] The purpose of this invention is to provide a mouse heart radiation injury mold based on 3D printing and molding technology, in order to solve the problem mentioned in the background art that the current device cannot accurately calculate the true data of the radiation source through the lead plate radiation hole, resulting in insufficient accuracy of radiation to the heart.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a mouse heart radiation injury mold based on 3D printing and molding technology, including a frame structure, which is a hollow, topless cuboid structure; the frame structure is assembled from two left and right connecting parts, which facilitates the early processing and later storage; and two rows of support members, which are symmetrically placed on the two side walls of the frame structure.

[0006] Preferably, each connector includes a base plate and edge plates disposed at three adjacent edges of the base plate, and the openings of each connector are spliced ​​together to form a frame structure, with the thickness of the base plate and edge plates being 0.5cm.

[0007] Preferably, one of the connectors has connecting buckles fixed on the edge plates on both sides, while the other connector has insert buckles fixed on the edge plates on both sides. The connecting buckles and insert buckles engage to ensure the stability of the frame structure after assembly.

[0008] Preferably, the support member is a column, with a fixing plate fixed at the bottom section of the column, and a stud fixed at the bottom of the fixing plate. A screw hole for the stud to be screwed into is provided on the top surface of the base plate, thereby realizing the detachable installation of the column. During disassembly and assembly, the angle of the fixing plate can be used to detect whether the specified angle of the fastening installation has been achieved. The cross-section of the fixing plate is a square with a missing corner.

[0009] Preferably, each column of the support consists of a column, with the column in the middle being vertical.

[0010] Preferably, the inclination angles of the nine columns constituting each column of the support member are 80.65°, 82.96°, 85.30°, 87.65°, 90°, 87.65°, 85.30°, 82.96°, and 80.65°.

[0011] Preferably, the height of the column is higher than the height of the edge plate, and the column is cylindrical.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] This invention utilizes 3D modeling to calculate the precise irradiation data of a mouse heart through a radiation hole in a lead plate, shielded by a lead plate. Based on the 3D structural data, a lead plate mold is 3D printed. Using low-melting-point lead, a high-precision mold is created, allowing X-rays to precisely pass through the radiation hole to form a 1cm diameter circular irradiation field, enabling precise radiotherapy to the heart. Other parts of the body receive even lower radiation doses under the protection of a 3cm thick lead plate, resulting in a lower animal mortality rate and a closer approximation to clinical practice. This invention can create models for 18 mice at once, significantly saving time and making the operation more convenient. Attached Figure Description

[0014] Figure 1 This is the front view of the three-dimensional model of this utility model;

[0015] Figure 2 This is a schematic diagram of the structure of this utility model;

[0016] Figure 3This is a schematic diagram of the lead plate of this utility model;

[0017] Figure 4 This utility model Figure 2 Top view;

[0018] Figure 5 This is a schematic diagram showing the arrangement of the screw holes on the base plate of this utility model;

[0019] Figure 6 This is a schematic diagram showing the connection between the stud and the column of this utility model.

[0020] In the picture:

[0021] 1. Radiation source; 2. Radiation; 3. Lead plate; 31. Radiation hole; 32. Positioning marker; 4. Circular radiation field; 5. Radiation plane; 6. Mouse model; 7. Base plate; 8. Edge plate; 9. Connecting buckle; 10. Post; 11. Fixing plate; 12. Snap buckle; 13. Screw hole; 14. Stud. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figures 1 to 6 This utility model provides a technical solution: a mouse heart radiation injury mold based on 3D printing and molding technology, including...

[0024] The frame structure is a hollow, open-top rectangular prism.

[0025] The frame structure is assembled from two connecting pieces on the left and right sides, which facilitates both the initial processing and the later storage.

[0026] The support components are arranged in two rows, and the two rows of support components are symmetrically placed on the two side walls of the frame structure.

[0027] In this embodiment, preferably, each connector includes a base plate 7 and an edge plate 8 disposed at three adjacent edges of the base plate 7. The openings of each connector are spliced ​​together to form a frame structure. The thickness of the base plate 7 and the edge plate 8 is 0.5cm.

[0028] In this embodiment, preferably, connecting buckles 9 are fixed on the edge plates 8 on both sides of one connector, while insert buckles 12 are fixed on the edge plates 8 on both sides of the other connector. The connecting buckles 9 and insert buckles 12 are engaged to ensure the stability of the frame structure after splicing.

[0029] In this embodiment, preferably, the support member is a column 10, a fixing plate 11 is fixedly provided at the bottom section of the column 10, and a stud 14 is fixedly provided at the bottom of the fixing plate 11. A screw hole 13 for the stud 14 to be screwed into is provided on the top surface of the base plate 7, so as to realize the detachable installation of the column 10. During disassembly and assembly, the angle of the fixing plate 11 can be used to detect whether the specified angle of the fastening installation has been reached. The cross-section of the fixing plate 11 is a square with a missing corner.

[0030] In this embodiment, preferably, each column of support consists of 9 columns 10, with the column 10 in the middle being vertical.

[0031] In this embodiment, preferably, the inclination angles of the nine columns 10 constituting each row of support members are 80.65°, 82.96°, 85.30°, 87.65°, 90°, 87.65°, 85.30°, 82.96°, and 80.65°.

[0032] In this embodiment, preferably, the height of the column 10 is higher than the height of the edge plate 8, and the column 10 is cylindrical. The processing steps of this device are as follows:

[0033] Step 1: The 3D image processing software simulates the radiation from source 1 through the radiation hole 31 on lead plate 3 to the table surface, forming a realistic scene of a circular radiation field 4 with a diameter of 1cm. The radiation source is 99cm from the radiation plane 5, the lead plate 3 is 3cm thick, and the lower surface of lead plate 3 is 9cm from the radiation plane 5. In the top view of the radiation field, the total irradiation field is 41*17cm, with nine circular radiation fields 4 with a diameter of 1cm in each of two rows. The center-to-center distance between two adjacent circular radiation fields 4 in the same row is 4cm, and the center-to-center distance between corresponding circular radiation fields 4 is interleaved to 12cm. The center of the outermost circular radiation field 4 is 2.5cm from the long side and 4.5cm from the short side of the total irradiation field.

[0034] Step 2: Extract the structural data of the radial aperture 31, including aperture diameter, angle, etc.;

[0035] Step 3: Establish the three-dimensional structure of the mold. Based on the simulation of the three-dimensional structure of lead plate 3 in Step 1, construct the three-dimensional structure of the mold in reverse. The column 10 that can form radial holes in the mold is lengthened by 0.5cm on the original basis.

[0036] Step 4: Import the 3D STL data of the mold made in Step 3 into the 3D printer to print the lead plate mold. The printing accuracy is 0.3mm. In order to facilitate demolding, the column 10 in the mold is made into a detachable type. At the same time, the 3D printing material is ABS material, which has good plasticity and high temperature resistance, and can be used as a low melting point lead mold, which is not easy to soften.

[0037] Step 5: Polish the mold;

[0038] Step 6: Place the low-melting-point lead into an aluminum pot and heat it until it melts;

[0039] Step 7: Place the lead plate mold horizontally, and slowly pour the melted low-melting-point lead into the mold;

[0040] Step 8: After 1-2 hours at room temperature, the low-melting-point lead solidifies, and the mold is then removed.

[0041] Step 9: Make wooden pads with corresponding parameters. The height of the wooden pads is 10cm. Place one pad on each side to raise lead plate 3 so that its lower surface is about 9cm away from the radiotherapy plane (mouse heart plane).

[0042] Step 10: Place a wooden pad on the radiation platform in the radiotherapy room, and place the prepared lead plate 3 on it, so that the positioning mark 32 coincides with the intersection of the laser crosshairs of the radiotherapy laser positioning system.

[0043] Step 11: In a dark room, check whether a circular radiation field 4 with a diameter of 1 cm is formed on the radiation plane 5 under the lead plate 3;

[0044] Experimental mice (6-8 week old male C57 / BL6 mice) were fasted for 12 hours before irradiation but allowed free access to water. Before modeling, the chest skin was prepared to observe the location of the heartbeat, and the heart position was marked on the skin with a 1 cm diameter circle. After anesthesia (10% chloral hydrate, intraperitoneal injection), the mice were placed on the radiation table in a supine position with their heads facing outwards. In a dark room, the position of the mice was adjusted so that the heart mark coincided with the circular radiation field 4. The mouse heart was irradiated with 6MV X-rays, a total dose of 20 Gy, and a dose rate of 300 cGy / min. The source-skin distance (SSD) was 100 cm, and the irradiation field was 40 cm × 15 cm.

[0045] Although embodiments of the present invention have been shown and described (see the detailed description above), it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A mouse cardiac radiation injury mold based on 3D printing and molding technology, characterized in that: include The frame structure is a hollow, open-top rectangular prism. The frame structure is assembled from two connecting pieces on the left and right sides; The support components are arranged in two rows, and the two rows of support components are symmetrically placed on the two side walls of the frame structure.

2. The mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 1, characterized in that: Each connector includes a base plate (7) and an edge plate (8) disposed at three adjacent edges of the base plate (7). The openings of each connector are spliced ​​together to form a frame structure.

3. A mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 2, characterized in that: A connecting buckle (9) is fixed on the edge plate (8) on both sides of one of the connectors, and a buckle (12) is fixed on the edge plate (8) on both sides of the other connector. The connecting buckle (9) and the buckle (12) are engaged and connected.

4. A mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 2, characterized in that: The support is a column (10), a fixing plate (11) is fixed at the bottom section of the column (10), and a stud (14) is fixed at the bottom of the fixing plate (11). A screw hole (13) for the stud (14) to be screwed into is provided on the top surface of the base plate (7).

5. A mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 4, characterized in that: Each column of the support consists of 9 columns (10), with the column (10) in the middle position being vertical.

6. A mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 5, characterized in that: The inclination angles of the nine columns (10) constituting each column of the support are 80.65°, 82.96°, 85.30°, 87.65°, 90°, 87.65°, 85.30°, 82.96°, and 80.65°.

7. A mouse cardiac radiation injury mold based on 3D printing and molding technology according to claim 4, characterized in that: The height of the column (10) is higher than the height of the edge plate (8), and the column (10) is cylindrical.

Images