A cell culture device for constructing a cell model

Abstract

This invention relates to the field of cell culture, specifically disclosing a cell culture device for constructing cell models, comprising: a culture unit, a medium circulation unit, and a control unit. This invention achieves differentiated regulation of the microenvironment for different cell types by setting up three independent cell culture chambers in the culture unit. Simultaneously, the medium circulation unit continuously filters and collects cell metabolites through circulating media, preventing the accumulation of metabolic waste that could lead to pH imbalance in the culture medium, further affecting cell survival and experimental reproducibility. Furthermore, this invention includes a partition with a functional membrane between the medium circulation unit and the cell culture chambers, allowing for controlled cell interaction by selecting different types of functional membranes.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, and more specifically, to a cell culture device for constructing cell models. Background Technology

[0002] As clinical research deepens, "comorbidity" (i.e., the simultaneous presence of two or more chronic diseases in the same patient) has become a key focus in the medical field. For example, patients with type 2 diabetes have a 30%-50% higher probability of developing colorectal cancer than healthy individuals, and the pathological processes of the two diseases can mutually promote each other (e.g., a high-glucose microenvironment accelerates tumor cell proliferation, while tumor inflammatory factors inhibit pancreatic β-cell function). To accurately simulate these complex pathological relationships, the research field urgently needs to construct "comorbidity cell models," which recreate the dynamic interactions and microenvironmental influences between cells in vivo through in vitro co-culture of various disease-related cells. The core technological bottleneck in constructing comorbidity cell models lies in how to simultaneously meet the "differentiated microenvironmental needs of different cells" and "controllable interactions between cells," which places stringent demands on existing cell co-culture devices.

[0003] Currently, traditional mixed culture devices are single-chamber designs that directly mix and seed multiple cell types in the same culture medium (such as conventional cell culture dishes and multi-well plates). Their core drawback is: The microenvironment cannot be differentiated: the needs of comorbid cells for the microenvironment vary significantly, while a single chamber can only provide uniform temperature and nutritional conditions, resulting in some cells experiencing decreased activity or phenotypic abnormalities due to microenvironmental discomfort, and failing to truly reproduce the physiological characteristics of cells under comorbid conditions; Cell-cell interactions are uncontrollable: the ways in which cells interact (such as paracrine signaling and direct physical contact) are completely random, making it impossible to distinguish between "targeted interactions between specific cells" (such as indirect signaling effects between tumor cells and pancreatic β cells) and "non-specific mixed interference," which makes it impossible to accurately locate key interaction pathways in comorbidity mechanism research. Accumulation of metabolic waste: When multiple cells are cultured together, the rate of production of metabolic waste (such as lactic acid and ammonia) increases significantly. However, single-chamber culture lacks a targeted waste removal pathway, which can easily lead to pH imbalance in the culture medium, further affecting cell survival and experimental reproducibility.

[0004] Therefore, a cell culture device for constructing cell models is needed to address the problems of existing cell culture devices, such as the inability to differentiate and regulate the microenvironment, uncontrollable cell interactions, and accumulation of metabolic waste. Summary of the Invention

[0005] In view of this, the present invention proposes a cell culture device for constructing cell models, aiming to solve at least one of the problems in the background art described above.

[0006] This invention provides a cell culture device for constructing cell models, comprising: a culture unit, a medium circulation unit, and a control unit; The culture unit is a cylindrical shell used for culturing cells; wherein, The culture unit includes: a substrate, which is a circular disk; three cell culture chambers, which are cylindrical cavities, on the top surface of the substrate; and a sealing cap, which is a cylindrical cap and is sealed to the outer periphery of the top surface of the substrate, and the sealing cap is used to isolate the cell culture chamber from the external environment. The media circulation unit is a cylindrical shell, with its top surface sealed to the bottom surface of the substrate. The media circulation unit is used to circulate the media and filter metabolic products. The magnetic stirring unit is cylindrical and located below the medium circulation unit. It is fixedly connected to the medium circulation unit and is used to magnetically stir the medium in the medium circulation unit. The control unit is located below the magnetic stirring unit and is electrically connected to the culture unit, the magnetic stirring unit, and the media circulation unit. It is used to control the operation of the equipment and adjust the equipment parameters.

[0007] Furthermore, a through hole and a mounting groove are provided at the position corresponding to the cell culture chamber on the top surface of the substrate, and a partition is detachably installed in the mounting groove.

[0008] Furthermore, the partition includes: Mounting plate, the shape of which fits into the mounting groove; A functional membrane is disposed on the mounting plate at a position corresponding to the through hole, and the functional membrane is used to enable the cell culture chamber to interact with the medium. A pull-out plate is located at the end of the mounting plate away from the base.

[0009] Furthermore, the outer surface of the cell culture chamber is covered with a heating jacket, which is used to regulate the culture temperature.

[0010] Furthermore, a pipeline lumen is also provided on the substrate, and the pipeline lumen is located in the middle of the cell culture chamber described in 3. The top of the pipeline cavity is provided with three first inlet pipes. The first inlet pipes extend out of the sealing cap and are connected to the external injection device. The first inlet pipes are used to deliver the externally prepared cell culture medium. The pipeline cavity sidewall is provided with three first liquid outlet tubes, which are respectively connected to the cell culture cavity. The first liquid outlet tubes are used to transport the externally prepared cell culture medium to the corresponding cell culture cavity.

[0011] Furthermore, a sealing strip is provided at the connection between the substrate and the sealing cover, and the sealing strip is used to isolate the external environment.

[0012] Furthermore, three inoculation holes are provided at the top of the sealing cap corresponding to the cell culture chamber described in section 3. Each inoculation hole is filled with a sealing plug and is used to inoculate external cells into the cell culture chamber.

[0013] Furthermore, the medium circulation unit includes: The circulation chamber is a cylindrical shell, the top of which is sealed to the base, and the circulation chamber is used to hold the circulation medium. A circulation control module is disposed on the outer wall of the circulation chamber. The circulation control module is used for circulating media, filtering cell metabolites, and injecting comorbidity intervention factors. The circulation control module has a fourth inlet pipe and multiple third inlet pipes on its side away from the circulation chamber. The fourth inlet pipe is used to draw in the medium, and the third inlet pipes are used to draw in the comorbidity intervention factor. Multiple third outlet pipes are provided on the side of the circulation control module perpendicular to the circulation chamber. One end of each third outlet pipe is connected to the circulation control module, and the other end is connected to the circulation chamber. A second inlet pipe and a second outlet pipe are provided on the other side of the circulation control module perpendicular to the circulation chamber. One end of each second inlet pipe and second outlet pipe is connected to the circulation control module, and the other end is connected to the circulation chamber.

[0014] Furthermore, an integrated sensor is provided on the inner wall of the cell culture chamber described in 3 to detect environmental information within the cell culture chamber.

[0015] Furthermore, the mounting plate is provided with a limiting block, and the mounting groove is provided with a limiting groove of a corresponding shape at the corresponding position, and the limiting block is engaged with the limiting groove.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows: 1. This utility model is equipped with three independent cell culture chambers, each with a heating jacket, which allows for independent temperature control of each cell culture chamber. The three independent cell culture chambers also enable the targeted injection of nutrients and specific culture media according to the cell culture type, solving the problem that traditional culture devices cannot differentiate and control the microenvironment.

[0017] 2. This utility model is equipped with a media circulation unit. Through the circulation medium, cell metabolic products can be continuously filtered and collected, avoiding the accumulation of metabolic waste that leads to pH imbalance of the culture medium, which further affects cell survival and experimental repeatability.

[0018] 3. This invention features a partition with a functional membrane between the media circulation unit and the cell culture chamber, which allows for controlled cell interaction by selecting different types of functional membranes. Attached Figure Description

[0019] Various other advantages and benefits will become apparent to those skilled in the art upon reading the following detailed description of preferred embodiments. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings: Figure 1 A three-dimensional structural diagram of a cell culture apparatus for constructing a cell model provided in one embodiment of the present invention; Figure 2 This is an internal structural diagram of a culture unit provided in one embodiment of the present invention; Figure 3 A three-dimensional structural diagram of a media circulation unit provided in one embodiment of this utility model; Figure 4 A perspective structural view of a partition provided in one embodiment of this utility model; Figure 5 A cross-sectional view of the partition and mounting groove provided in one embodiment of this utility model; Figure 6 This is a structural diagram of the internal structure of a cell culture chamber provided in one embodiment of the present invention.

[0020] In the diagram: 100 - Culture unit; 110 - Sealing cap; 111 - Sealing plug; 112 - Inoculation well; 120 - Cell culture chamber; 121 - Heating jacket; 130 - Piping chamber; 131 - First outlet pipe; 132 - First inlet pipe; 140 - Substrate; 141 - Sealing strip; 142 - Mounting groove; 143 - Through hole; 144 - Limiting groove; 150 - Partition; 151 - Mounting plate; 152 - Functional membrane; 153 - Limiting block; 154 - Pull-out plate; 200 - Media circulation unit; 210 - Circulation chamber; 220 - Circulation control module; 221 - Fourth inlet pipe; 222 - Second inlet pipe; 223 - Second outlet pipe; 224 - Third outlet pipe; 225 - Third inlet pipe; 300 - Magnetic stirring unit; 400 - Control unit. Detailed Implementation

[0021] The specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0022] In the description of this application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation; or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0023] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0024] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0025] See Figure 1-2 As shown, this embodiment provides a cell culture apparatus for constructing cell models, including: Culture unit 100, media circulation unit 200, and control unit 400; The culture unit 100 is a cylindrical shell used for culturing cells; wherein, The culture unit 100 includes: a substrate 140, which is a circular disk; a cell culture chamber 120, which is a cylindrical cavity, with three cell culture chambers 120 disposed on the top surface of the substrate 140; and a sealing cover 110, which is a cylindrical cover and is sealed to the outer periphery of the top surface of the substrate 140. The sealing cover 110 is used to isolate the cell culture chamber 120 from the external environment. The media circulation unit 200 is a cylindrical shell. The top surface of the media circulation unit 200 is sealed to the bottom surface of the base 140. The media circulation unit 200 is used to circulate media and filter metabolic products. The magnetic stirring unit 300 is cylindrical and located below the medium circulation unit 200. It is fixedly connected to the medium circulation unit 200 and is used to magnetically stir the medium in the medium circulation unit 200. The control unit 400 is located below the magnetic stirring unit 300 and is electrically connected to the culture unit 100, the magnetic stirring unit 300, and the media circulation unit 200. It is used to control the operation of the equipment and adjust the equipment parameters.

[0026] Specifically, the cell culture chamber 120 and the sealing cap 110 are made of transparent material to facilitate observation of cell generation.

[0027] Specifically, when constructing a cell comorbidity model, different cells can be seeded into different cell culture chambers 120 for culture. During the culture process, cell metabolites fall into the lower medium circulation unit 200, and the cell metabolites are continuously filtered through circulation. A magnetic stirring unit 300 is set below the medium circulation unit 200. The magnetic stirring unit 300 can stir the medium and control the medium temperature, reducing the influence of the medium on the temperature of the cell culture chamber 120.

[0028] It is understood that this utility model has three independent cell culture chambers 120, each equipped with a heating jacket 121, which allows for independent temperature control of each cell culture chamber 120. The three independent cell culture chambers 120 also enable the targeted injection of nutrients and specific culture media according to the cell culture type, solving the problem that traditional culture devices cannot differentiate and control the microenvironment.

[0029] It is understood that this invention includes a media circulation unit 200, which continuously filters and collects cell metabolic products through circulating media, avoiding the accumulation of metabolic waste that could lead to pH imbalance in the culture medium and further affect cell survival and experimental reproducibility.

[0030] It is understood that the present invention provides a partition 150 with a functional membrane 152 between the medium circulation unit 200 and the cell culture chamber 120, and controllable interaction of cells can be achieved by selecting different types of functional membranes 152.

[0031] See Figure 2-4 As shown, in some embodiments provided by this utility model, a through hole 143 and an installation groove 142 are provided at the position corresponding to the cell culture chamber 120 on the top surface of the substrate 140, and a partition 150 is detachably installed in the installation groove 142.

[0032] Partition 150 includes: Mounting plate 151, the shape of which fits into mounting groove 142; A functional membrane 152 is disposed on the mounting plate 151 at a position corresponding to the through hole 143. The functional membrane 152 is used to enable the cell culture chamber 120 to interact with the medium. A pull-out plate 154 is located at the end of the mounting plate 151 away from the base 140.

[0033] Specifically, the septum 150 is classified according to the type of its functional membrane 152 as follows: semipermeable membrane septum 150 (pore size 0.22-0.45μm): only allows small molecule signals (such as cytokines and metabolites) to pass through, simulating "indirect paracrine interaction" (such as the interaction between tumor cells and pancreatic β cells through inflammatory factors); microchannel septum 150 (pore size 5-10μm): allows cell processes (such as neuronal axons and immune cell pseudopodia) to pass through, achieving "local direct contact" (such as the physical connection between vascular endothelial cells and smooth muscle cells).

[0034] Specifically, one side of the functional membrane 152 is in contact with the cell culture system in the cell culture chamber 120, and the other side is in contact with the medium in the medium circulation unit 200. During cell culture, cell metabolites enter the medium through the functional membrane 152 and are then filtered through medium circulation to achieve continuous removal of cell metabolites.

[0035] See Figure 1 As shown, in some embodiments provided by this utility model, the outer side of the cell culture chamber 120 is wrapped with a heating sleeve 121, which is used to regulate the culture temperature.

[0036] Specifically, the heating jacket 121 is electrically connected to the control unit 400, and the temperature of the three heating jackets 121 can be set through the control unit 400.

[0037] See Figure 1 As shown, in some embodiments provided by this utility model, a pipeline cavity 130 is also provided on the substrate 140, and the pipeline cavity 130 is located in the middle of the 3-cell culture cavity 120. The top of the pipeline cavity 130 is provided with three first liquid inlet pipes 132. The three first liquid inlet pipes 132 extend out of the sealing cap 110 and are connected to the external injection device. The first liquid inlet pipes 132 are used to deliver externally prepared cell culture medium. The side wall of the pipeline cavity 130 is provided with three first liquid outlet pipes 131, which are respectively connected to the cell culture cavity 120. The first liquid outlet pipes 131 are used to transport the externally prepared cell culture medium to the corresponding cell culture cavity 120.

[0038] Specifically, the pipeline cavity 130 is used to hold and fix the first inlet pipe 132 and the first outlet pipe 131. The external injection device injects specific culture medium and nutrients into the corresponding cell culture cavity 120 through the first inlet pipe 132 and the first outlet pipe 131. At the same time, a solenoid valve is installed inside the connection between the first inlet pipe 132 and the external injection device. When injection is not required, the solenoid valve is closed to prevent external substances from entering the cell culture system.

[0039] See Figure 2As shown, in some embodiments of the present invention, a sealing strip 141 is provided at the connection between the base 140 and the sealing cover 110, and the sealing strip 141 is used to isolate the external environment.

[0040] See Figure 1 As shown, in some embodiments provided by this utility model, three inoculation holes 112 are provided at the top of the sealing cap 110 and at the corresponding position of the cell culture chamber 120. The inoculation holes 112 are filled with sealing plugs 111, and the inoculation holes 112 are used to inoculate external cells into the cell culture chamber 120.

[0041] Specifically, when using the method, first inject the culture medium, then remove the sealing plug 111 in the inoculation well 112, and inoculate the cells into the culture medium for culture.

[0042] See Figure 3 As shown, in some embodiments provided by this utility model, the medium circulation unit 200 includes: The circulation chamber 210 is a cylindrical shell. The top of the circulation chamber 210 is sealed to the base 140. The circulation chamber 210 is used to hold the circulation medium. The circulation control module 220 is located on the outer wall of the circulation chamber 210. The circulation control module 220 is used for circulating media, filtering cell metabolites, and injecting comorbidity intervention factors. On the side of the circulation control module 220 away from the circulation chamber 210, there is a fourth inlet pipe 221 and multiple third inlet pipes 225. The fourth inlet pipe 221 is used to absorb the medium, and the third inlet pipes 225 are used to absorb the comorbidity intervention factor. On the side of the circulation control module 220 perpendicular to the circulation chamber 210, there are multiple third outlet pipes 224. One end of the third outlet pipe 224 is connected to the circulation control module 220, and the other end is connected to the circulation chamber 210. On the other side of the circulation control module 220 perpendicular to the circulation chamber 210, there are a second inlet pipe 222 and a second outlet pipe 223. One end of the second inlet pipe 222 and the second outlet pipe 223 is connected to the circulation control module 220, and the other end is connected to the circulation chamber 210.

[0043] Specifically, the medium is a basic cell culture medium. Metabolic waste and cytokines generated during cell culture enter the medium through the functional membrane 152, and then enter the circulation control module 220 through the second outlet pipe 223. At this time, the metabolic products are filtered, and the medium containing cytokines re-enters the circulation chamber 210 through the second inlet pipe 222 to remove metabolic substances. At the same time, the cytokines in the medium can enter other cell culture chambers 120 through the functional membrane 152 to achieve cell interaction.

[0044] Specifically, during the culture process, the circulation control module 220 draws the required comorbidity intervention factor through the third inlet pipe 225 and then injects it into the medium through the third inlet pipe 225. The comorbidity intervention factor in the medium then enters the cell culture chamber 120 through the functional membrane 152. During this period, the magnetic stirring unit 300 stirs the medium by magnetically controlling the stir bar in the circulation chamber 210 to ensure that the concentration of the comorbidity intervention factor is uniform.

[0045] See Figure 6 As shown, in some embodiments provided by this utility model, an integrated sensor is provided on the inner wall of the cell culture chamber 120 to detect environmental information inside the cell culture chamber 120.

[0046] Specifically, the integrated sensor is electrically connected to the control unit 400. The integrated sensor can detect the pH value and temperature in each cell culture chamber 120. When the temperature in the cell culture chamber 120 exceeds or falls below the preset temperature range, the control unit 400 controls the heating jacket 121 to heat or cool down. When the pH value in the cell culture chamber 120 exceeds or falls below the preset pH range, the control unit 400 will issue an alarm, and the staff can then use an external injection device to inject pH adjuster through the first inlet pipe 132 and the first outlet pipe 131.

[0047] See Figure 4-5 As shown, in some embodiments provided by this utility model, a limiting block 153 is provided on the mounting plate 151, and a limiting groove 144 of a corresponding shape is provided at the corresponding position of the mounting groove 142, and the limiting block 153 and the limiting groove 144 are engaged.

[0048] Specifically, when the partition 150 is installed into the mounting groove 142, the partition 150 is directly inserted into the mounting groove 142. When the limiting block 153 engages with the limiting groove 144, it indicates that the installation is in place.

[0049] Specifically, the method of use and principle of this utility model are as follows: S1. Install the partition 150 according to the required number of cells to be cultured, and inject the cell culture medium into the cell culture chamber 120 through the first inlet pipe 132 and the first outlet pipe 131 via the control unit 400. Then, inject the medium into the circulation chamber 210 through the fourth inlet pipe 221, and then set the preset temperature and pH. S2. Remove the sealing plug 111 and inoculate the cells into the cell culture chamber 120 through the inoculation hole 112. Then, tighten the sealing plug 111 to carry out cell culture.

[0050] During culture, metabolic waste products and cytokines produced by the cells enter the medium through the functional membrane 152, and then enter the circulation control module 220 through the second outlet tube 223. At this time, the metabolic products are filtered out, and the medium containing cytokines re-enters the circulation chamber 210 through the second inlet tube 222 to remove metabolic substances. At the same time, the cytokines in the medium can enter other cell culture chambers 120 through the functional membrane 152 to achieve cell interaction. During the culture process, the circulation control module 220 draws the required comorbidity intervention factor through the third inlet tube 225 and then injects it into the medium through the third inlet tube 225. The comorbidity intervention factor in the medium then enters the cell culture chamber 120 through the functional membrane 152.

[0051] Meanwhile, integrated sensors monitor the pH value and temperature in each cell culture chamber 120 in real time. When the temperature in the cell culture chamber 120 exceeds or falls below the preset temperature range, the control unit 400 controls the heating jacket 121 to heat or cool down. When the pH value in the cell culture chamber 120 exceeds or falls below the preset pH range, the control unit 400 will issue an alarm, and staff can then use an external injection device to inject pH adjuster through the first inlet pipe 132 and the first outlet pipe 131.

[0052] It will be understood by those skilled in the art that the above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell culture apparatus for constructing a cell model, characterized in that, include: Culture unit, media circulation unit, and control unit; The culture unit is a cylindrical shell used for culturing cells; wherein, The culture unit includes: a substrate, which is a circular disk; three cell culture chambers, which are cylindrical cavities, on the top surface of the substrate; and a sealing cap, which is a cylindrical cap and is sealed to the outer periphery of the top surface of the substrate, and the sealing cap is used to isolate the cell culture chamber from the external environment. The media circulation unit is a cylindrical shell. The top surface of the media circulation unit is sealed to the bottom surface of the substrate. The media circulation unit is used to circulate the media and filter metabolic products. The magnetic stirring unit is cylindrical and located below the medium circulation unit. It is fixedly connected to the medium circulation unit and is used to magnetically stir the medium in the medium circulation unit. The control unit is located below the magnetic stirring unit and is electrically connected to the culture unit, the magnetic stirring unit, and the media circulation unit. It is used to control the operation of the equipment and adjust the equipment parameters.

2. The cell culture apparatus for constructing cell models according to claim 1, characterized in that, The top surface of the substrate is provided with a through hole and a mounting groove at the position corresponding to the cell culture chamber, and a partition is detachably installed in the mounting groove.

3. The cell culture apparatus for constructing cell models according to claim 2, characterized in that, The partition includes: Mounting plate, the shape of which fits into the mounting groove; A functional membrane is disposed on the mounting plate at a position corresponding to the through hole, and the functional membrane is used to enable the cell culture chamber to interact with the medium. A pull-out plate is located at the end of the mounting plate away from the base.

4. The cell culture apparatus for constructing cell models according to claim 3, characterized in that, The outer surface of the cell culture chamber is covered with a heating jacket, which is used to regulate the culture temperature.

5. The cell culture apparatus for constructing cell models according to claim 4, characterized in that, The substrate is also provided with a pipeline cavity, which is located in the middle of the cell culture cavity described in 3. The top of the pipeline cavity is provided with three first inlet pipes. The first inlet pipes extend out of the sealing cap and communicate with the external injection device. The first inlet pipes are used to deliver the externally prepared cell culture medium. The pipeline cavity sidewall is provided with three first liquid outlet tubes, which are respectively connected to the cell culture cavity. The first liquid outlet tubes are used to transport the externally prepared cell culture medium to the corresponding cell culture cavity.

6. The cell culture apparatus for constructing cell models according to claim 5, characterized in that, A sealing strip is provided at the connection between the substrate and the sealing cover, and the sealing strip is used to isolate the external environment.

7. The cell culture apparatus for constructing cell models according to claim 6, characterized in that, The top of the sealing cap has three inoculation holes at the position corresponding to the cell culture chamber described in section 3. Each inoculation hole is filled with a sealing plug and is used to inoculate external cells into the cell culture chamber.

8. The cell culture apparatus for constructing cell models according to claim 7, characterized in that, The medium circulation unit includes: The circulation chamber is a cylindrical shell, the top of which is sealed to the base, and the circulation chamber is used to hold the circulation medium. A circulation control module is disposed on the outer wall of the circulation chamber. The circulation control module is used to circulate the medium, filter cell metabolites, and inject comorbidity intervention factors. The circulation control module has a fourth inlet pipe and multiple third inlet pipes on its side away from the circulation chamber. The fourth inlet pipe is used to draw in the medium, and the third inlet pipes are used to draw in the comorbidity intervention factor. Multiple third outlet pipes are provided on the side of the circulation control module perpendicular to the circulation chamber. One end of each third outlet pipe is connected to the circulation control module, and the other end is connected to the circulation chamber. A second inlet pipe and a second outlet pipe are provided on the other side of the circulation control module perpendicular to the circulation chamber. One end of each second inlet pipe and second outlet pipe is connected to the circulation control module, and the other end is connected to the circulation chamber.

9. The cell culture apparatus for constructing cell models according to claim 8, characterized in that, 3. An integrated sensor is provided on the inner wall of the cell culture chamber to detect environmental information inside the cell culture chamber.

10. The cell culture apparatus for constructing cell models according to claim 9, characterized in that, The mounting plate is provided with a limiting block, and the mounting groove is provided with a limiting groove of a corresponding shape at the corresponding position, and the limiting block is engaged with the limiting groove.

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