A cell culture bag

Abstract

The utility model relates to cell culture bag technical field discloses a cell culture bag, including the bag body, the one side of bag body is provided with a plurality of connecting heads, a plurality of connecting head one side is connected with liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device, cell enrichment and liquid supplementing device include the connecting pipe of setting up in corresponding connecting head one side, through setting up liquid inlet pipe subassembly, negative pressure needleless connector and cell enrichment and liquid supplementing device in the bag body one side, realized the integration function of liquid inlet, negative pressure operation, cell enrichment and liquid supplementing in cell culture process, the valve component design of cell enrichment and liquid supplementing device, can accurate control cell enrichment and culture medium supplement's process, avoid the interference caused by both simultaneously, guarantee the purity when cell enrichment and the sterile environment when liquid supplementing, thereby improve cell subculture expansion culture's efficiency and stability, reduce the operation step, reduce the pollution risk.

Description

Technical Field

[0001] This utility model relates to the field of cell culture bag technology, and in particular to a cell culture bag. Background Technology

[0002] Cell culture bags are containers used for the in vitro culture of cells and tissues, and are widely used in life science research and cell therapy. They are primarily used for culturing suspension and adherent cells, have excellent air permeability, support high-density cell culture, and are suitable for culturing suspension cells such as lymphocytes and hybridomas. They have important applications in areas such as tumor research and drug screening, for example, by culturing tumor cells to evaluate the efficacy of anti-tumor drugs.

[0003] In existing technologies, cell culture systems often face problems such as cumbersome operation and high risk of contamination when changing and replenishing culture medium. In routine operations, it is necessary to first remove the old culture medium containing metabolic waste from the culture container using a pipette or syringe. This process requires frequently opening the sealed structure of the culture container, which not only increases the probability of external microbial invasion, but may also lead to cell loss due to improper operation. Utility Model Content

[0004] The purpose of this invention is to solve the problems existing in the prior art by proposing a cell culture bag.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A cell culture bag includes a bag body, with a plurality of connectors provided on one side of the bag body, and a liquid inlet tube assembly, a negative pressure needleless connector, and a cell enrichment and replenishment device respectively connected to one side of the plurality of connectors. The cell enrichment and replenishment device includes a connecting tube disposed on one side of a corresponding connector. One side of the connecting tube is connected to two feed tubes via a connector. The two feed tubes are respectively provided with a first filter and a second filter. The other side of the two feed tubes is connected to a flexible tube via a connector. The other end of the flexible tube is connected to a replenishment line and an enrichment line via a connector. Both feed pipes, replenishment pipes, and enrichment pipes are equipped with valve assemblies, allowing the liquid inside the bag to pass through the first filter and the enrichment pipe into the collection bag connected to one side of the enrichment pipe. The valve assemblies on the replenishment pipe and the second filter side are closed. After enrichment is completed, the valves of the enrichment pipe and the first filter are closed, while the valves of the replenishment pipe and the second filter are opened. The culture medium in the replenishment bag connected to one side of the replenishment pipe is filtered by the second filter and then replenished into the bag for continued subculturing and amplification culture.

[0006] Preferably, the connector is a tee pipe.

[0007] Preferably, a peristaltic pump is provided on the outer periphery of the hose to drive the movement of liquid in the feed pipe, replenishment pipe and enrichment pipe.

[0008] Preferably, the valve assembly is a one-way valve.

[0009] Preferably, the one-way valves on the first filter and enrichment pipeline side have the same one-way flow direction, the one-way valves on the second filter and replenishment pipeline side have the same one-way flow direction, and the one-way valves on the first filter and enrichment pipeline side and the one-way valves on the second filter and replenishment pipeline side have different one-way flow directions.

[0010] Preferably, the first filter and the second filter are 1.2 μm filter membranes and 0.2 μm filter membranes, respectively.

[0011] Preferably, the hose is integrally molded from silicone.

[0012] Preferably, the inlet pipe assembly includes an inlet pipe body connected to a connector, and a Luer connector is installed at the other end of the inlet pipe body.

[0013] Preferably, the bag body is formed by integral welding of 0.1mm PE film, and the bottom of the bag body has three openings, with several connectors disposed in the corresponding openings.

[0014] Preferably, the inner cavity of the bag is formed with at least one welded partition through a welded process, which divides the inner space of the bag into a first cavity and a second cavity.

[0015] Compared with the prior art, the beneficial effects of this utility model are: This invention integrates the functions of liquid inlet assembly, negative pressure needleless connector, and cell enrichment and replenishment during cell culture by setting an inlet tube assembly, a negative pressure connector, and a cell enrichment and replenishment device on one side of the bag. The valve assembly design of the cell enrichment and replenishment device can precisely control the process of cell enrichment and culture medium replenishment, avoiding interference caused by the simultaneous occurrence of the two, ensuring the purity of cells during enrichment and the sterile environment during replenishment, thereby improving the efficiency and stability of cell passage expansion culture, reducing operation steps, and reducing the risk of contamination. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of a cell culture bag proposed in this utility model; Figure 2 This is a schematic diagram of a flexible tube for a cell culture bag according to the present invention; Figure 3 This is a schematic diagram of a negative pressure needleless connector for a cell culture bag proposed in this utility model.

[0017] In the diagram: 1. Bag body; 2. Connector; 3. Negative pressure needleless connector; 4. Connecting tube; 5. Feed tube; 6. Hose; 7. First filter; 8. Second filter; 9. Liquid replenishment line; 10. Enrichment line; 11. T-connector; 12. Check valve; 13. Inlet tube body; 14. Luer connector; 15. Weak weld partition; 16. First cavity; 17. Second cavity; 18. Cell enrichment and liquid replenishment device; 19. Water stop clamp. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0019] Reference Figure 1-3 A cell culture bag includes a bag body 1, with a plurality of connectors 2 provided on one side of the bag body 1, and a liquid inlet tube assembly, a negative pressure needleless connector 3 and a cell enrichment and replenishment device 18 respectively connected to one side of the plurality of connectors 2. The cell enrichment and replenishment device 18 includes a connecting pipe 4 disposed on one side of the corresponding connector 2. One side of the connecting pipe 4 is connected to two feed pipes 5 via a connector. The two feed pipes 5 are respectively provided with a first filter 7 and a second filter 8. The other side of the two feed pipes 5 is connected to a hose 6 via a connector. The other end of the hose 6 is connected to a replenishment line 9 and an enrichment line 10 via a connector. Each of the two feed pipes 5, the replenishment pipe 9, and the enrichment pipe 10 is equipped with a valve assembly, which allows the liquid in the bag 1 to pass through the first filter 7 and the enrichment pipe 10 into the collection bag connected to one side of the enrichment pipe 10. The valve assembly on the replenishment pipe 9 and the second filter 8 is closed. After enrichment is completed, the valves of the enrichment pipe 10 and the first filter 7 are closed, and the valves of the replenishment pipe 9 and the second filter 8 are opened. The culture medium in the replenishment bag connected to one side of the replenishment pipe 9 is filtered by the second filter 8 and then replenished into the bag 1 for continued subculturing and amplification culture.

[0020] In use, this device integrates the functions of liquid inlet assembly, negative pressure operation, cell enrichment and replenishment during cell culture by setting up an inlet tube assembly, a negative pressure needleless connector 3, and a cell enrichment and replenishment device 18 on one side of the bag body 1. During cell culture, the initial culture medium and cells are added into the bag body 1 through the inlet tube assembly. The valve assembly design of the cell enrichment and replenishment device 18 can precisely control the process of cell enrichment and culture medium replenishment, avoiding interference caused by the simultaneous occurrence of the two, ensuring the purity of cell enrichment and the sterile environment during replenishment, thereby improving the efficiency and stability of cell passage and expansion culture, reducing operation steps, and reducing the risk of contamination. When sampling or adding cytokines is required, only a screw-type syringe needs to be connected to the negative pressure needleless connector 3, and the operation can be repeated without leakage risk, reducing the risk of contamination.

[0021] Furthermore, the connector is a three-way pipe 11. By setting the connector as a three-way pipe 11, the connection between the connecting pipe 4 and the two feed pipes 5, the two feed pipes 5 and the hose 6, and the hose 6 and the replenishment pipe 9 and enrichment pipe 10 is made simpler and more reliable. It can effectively divert or merge fluids, ensure smooth fluid transmission between the pipes, reduce fluid retention and leakage at the connection points, and improve the sealing performance and ease of operation of the entire device.

[0022] Furthermore, a peristaltic pump (not shown in the figure) is provided on the outer periphery of the tubing 6 to drive the movement of liquid in the feed tube 5, replenishment tube 9, and enrichment tube 10. When cell enrichment is performed, the peristaltic pump activates and acts on the tubing 6, squeezing the tubing to direct the fluid towards the enrichment tube 10. This drives the cell suspension in the bag 1 through the connecting tube 4, feed tube 5, first filter 7, tubing 6, and enrichment tube 10 into the collection bag. When replenishment is performed, the peristaltic pump changes direction, driving the culture medium through the feed tube 5, second filter 8, tubing 6, and replenishment tube 9 into the cell culture bag. Simultaneously, the fluid transfer speed and flow rate can be controlled by adjusting the speed of the peristaltic pump, allowing for stable and precise movement of liquid in the feed tube 5, replenishment tube 9, and enrichment tube 10. This avoids contamination caused by direct contact between the pump body and the fluid, while also allowing for flexible adjustment of the fluid transfer speed and flow rate to meet the fluid transfer requirements at different stages of cell enrichment and replenishment, ensuring operational controllability.

[0023] Furthermore, the valve assembly is a one-way valve 12. By using a one-way valve 12, the backflow of fluid in the pipeline can be effectively prevented, ensuring that the liquid in the bag 1 can only enter the collection bag through the first filter 7 and the enrichment pipeline 10 during cell enrichment, and that the culture medium can only enter the cell culture bag through the second filter 8 and the replenishment pipeline 9 during replenishment, thus avoiding cross-contamination and operation failure caused by reverse flow of fluid.

[0024] Furthermore, the one-way valve 12 on one side of the first filter 7 and the enrichment pipeline 10 has the same one-way flow direction, and the one-way valve 12 on one side of the second filter 8 and the replenishment pipeline 9 has the same one-way flow direction. However, the one-way valve 12 on one side of the first filter 7 and the enrichment pipeline 10 has a different one-way flow direction than the one-way valve 12 on one side of the second filter 8 and the replenishment pipeline 9. This further strengthens the independence of the cell enrichment and replenishment processes, completely avoiding fluid mixing between the two processes. It ensures that only the target material flows along the set path during the cell enrichment stage, and only the culture medium is transferred in the prescribed direction during the replenishment stage. This fundamentally eliminates the mutual interference between the two operations and ensures the orderly progress of cell culture.

[0025] Furthermore, the first filter 7 and the second filter 8 are 1.2μm and 0.2μm filter membranes, respectively. The first filter 7 uses a 1.2μm filter membrane. The one-way valve 12 on the enrichment line 10 and the 1.2μm filter membrane side is open, while the one-way valve 12 on the replenishment line 9 and the 0.2μm filter is closed. The culture medium in the culture bag enters the collection bag through the enrichment line 10, and the cells are retained by the 1.2μm filter membrane. The second filter 8 uses a 0.2μm filter membrane. The culture medium in the replenishment bag is sterilized and filtered by the 0.2μm filter before being added to the cell culture bag for continued passage and expansion culture, providing a clean growth environment for the cells and improving the success rate of cell culture.

[0026] Furthermore, the hose 6 is made of silicone in one piece, which has good flexibility and elasticity, and can work stably with the peristaltic pump, reducing fluid transmission problems caused by the material of the hose 6.

[0027] Furthermore, the inlet tube assembly includes an inlet tube body 13 connected to the connector 2. The other end of the inlet tube body 13 is equipped with a Luer connector 14. The inlet tube body 13 of the inlet tube assembly is equipped with a Luer connector 14, which enables it to be quickly connected to a variety of standard infusion devices, syringes, etc., improving the versatility and convenience of adding initial culture medium and cell operations into the bag 1. It also facilitates the rapid replacement of inlet equipment in different experimental scenarios, reducing operation time and improving work efficiency.

[0028] The outer periphery of the liquid inlet tube body 13 is also provided with a plastic water-stop clamp 19, which can precisely control the flow and interruption of liquid in the liquid inlet tube by manually adjusting the tightness of the clamp. When it is necessary to stop the liquid inlet operation, closing the water-stop clamp 19 can quickly block the liquid flow, avoid liquid leakage when changing the liquid inlet container or pausing the culture, and reduce the risk of pollution and waste of resources caused by liquid overflow.

[0029] Furthermore, the bag body 1 is formed using a one-piece welding process with a 0.1mm PE film. The bottom of the bag body 1 has three openings, and several connectors 2 are disposed within the corresponding openings. The PE film, with its excellent flexibility, chemical resistance, and biocompatibility, is suitable for cell growth. The one-piece welding process ensures the bag body 1's airtightness, preventing leakage. The 0.1mm thickness ensures both the strength of the bag body 1 and facilitates observation of cell growth within the bag. The three openings at the bottom, with connectors 2, provide a rational connection layout for the various devices and facilitate operation.

[0030] Furthermore, the inner cavity of the bag body 1 is formed with at least one welded partition 15 through a welded process. The welded partition 15 divides the internal space of the bag body 1 into a first cavity 16 and a second cavity 17. When the welded partition 15 is closed, the first cavity 16 and the second cavity 17 are not connected to each other. When it is necessary to expand the culture volume, the welded edge can be opened by squeezing to connect the two first cavities 16 and the second cavity 17. This increases the flexibility of the bag body 1 and can adapt to different culture scales and operational requirements in the cell culture process, thereby improving the utilization rate of the bag body 1.

[0031] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cell culture bag, comprising a bag body (1), characterized in that: A plurality of connectors (2) are provided on one side of the bag body (1), and one side of each connector (2) is respectively connected to an inlet tube assembly, a negative pressure needleless connector (3) and a cell enrichment and replenishment device (18). The cell enrichment and replenishment device (18) includes a connecting tube (4) disposed on one side of the corresponding connector (2). One side of the connecting tube (4) is connected to two feed tubes (5) via a connector. The two feed tubes (5) are respectively provided with a first filter (7) and a second filter (8). The other side of the two feed tubes (5) is connected to a flexible tube (6) via a connector. The other end of the flexible tube (6) is connected to a replenishment line (9) and an enrichment line (10) via a connector. The two feed pipes (5), the replenishment pipe (9), and the enrichment pipe (10) are all equipped with valve assemblies, which allow the liquid in the bag (1) to pass through the first filter (7) and the enrichment pipe (10) into the collection bag connected to one side of the enrichment pipe (10). The valve assemblies on the replenishment pipe (9) and the second filter (8) are closed. After enrichment is completed, the valves of the enrichment pipe (10) and the first filter (7) are closed, and the valves of the replenishment pipe (9) and the second filter (8) are opened. The culture medium in the replenishment bag connected to one side of the replenishment pipe (9) is filtered by the second filter (8) and then replenished into the bag (1) for continued subculture and amplification culture.

2. The cell culture bag according to claim 1, characterized in that: The connector is a tee pipe (11).

3. The cell culture bag according to claim 2, characterized in that: A peristaltic pump is provided on the outer periphery of the hose (6) to drive the movement of liquid in the feed pipe (5), replenishment pipe (9) and enrichment pipe (10).

4. A cell culture bag according to claim 3, characterized in that: The valve assembly is a one-way valve (12).

5. A cell culture bag according to claim 4, characterized in that: The one-way flow direction of the first filter (7) and the one-way valve (12) on the enrichment pipeline (10) side is the same, and the one-way flow direction of the second filter (8) and the one-way valve (12) on the replenishment pipeline (9) side is the same. The one-way flow direction of the one-way valve (12) on the first filter (7) and the one-way valve (12) on the enrichment pipeline (10) side is different from that of the one-way valve (12) on the second filter (8) and the one-way valve (12) on the replenishment pipeline (9) side.

6. A cell culture bag according to claim 5, characterized in that: The first filter (7) and the second filter (8) are 1.2 μm and 0.2 μm filter membranes, respectively.

7. A cell culture bag according to claim 6, characterized in that: The hose (6) is made of silicone in one piece.

8. A cell culture bag according to claim 7, characterized in that: The inlet pipe assembly includes an inlet pipe body (13) connected to the connector (2), and a Luer connector (14) is installed at the other end of the inlet pipe body (13).

9. A cell culture bag according to claim 8, characterized in that: The bag body (1) is formed by integral welding of 0.1mm PE film. The bottom of the bag body (1) has three openings, and several connectors (2) are set in the corresponding openings.

10. A cell culture bag according to claim 9, characterized in that: The inner cavity of the bag body (1) is formed with at least one welded partition (15) through a welded process, which divides the inner space of the bag body (1) into a first cavity (16) and a second cavity (17).

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