An nk cell cryopreservation apparatus

Abstract

A kind of NK cell freezing equipment, including cell freezing box, freezer, sealing door, the cell freezing box is connected sealing door by hinge, cell freezing box bottom is connected freezer, cell freezing box bottom plate has multiple exhaust holes, exhaust holes are evenly distributed on cell freezing box bottom plate, cell freezing box inside has protective box, protective box inside has auxiliary cavity, two freezing pipes are provided in auxiliary cavity inside, freezing pipe is symmetrically arranged, freezing pipe bottom passes through cell freezing box and is connected with freezer, air outlet pipe outside cell freezing box passes through protective box and is connected with exhaust pipe, freezing speed is fast, freezing effect is good, cell survival rate is high, access is convenient, wide application range, simple operation, it is convenient to adjust, structure is reasonable, effectively avoid that cell storage pipe 14 is inclined and spills, limiting effect is good.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering, and in particular to a novel NK cell cryopreservation device. Background Technology

[0002] An existing patent (publication number: CN 211861631U) discloses an NK cell cryopreservation device, including a box, a support plate, and a sealing cap. The device is characterized by: a positioning post vertically arranged at the bottom of the box; a positioning sleeve arranged on the back of the support plate; a positioning post vertically arranged on the front of the support plate; a support hole formed on the support plate; and the positioning sleeve on the support plate fitting onto the positioning post at the bottom of the box. The sealing cap is movably mounted on the box, and a limit block is provided inside the box. However, this device has a slow freezing speed and unsatisfactory freezing effect, and its structure needs improvement. Summary of the Invention

[0003] This invention addresses the aforementioned shortcomings of existing technologies by providing a novel NK cell cryopreservation device that offers excellent freezing performance, high cell survival rate, convenient storage and retrieval, wide applicability, simple operation, and effectively prevents cell storage tubes from tilting and spilling, while also providing good positioning.

[0004] The objective of this utility model is achieved through the following technical solution: A novel NK cell cryopreservation device includes a cell cryopreservation box, a freezer, and a sealed door. The cell cryopreservation box is connected to the sealed door via a hinge. The freezer is connected to the bottom of the cell cryopreservation box. The bottom plate of the cell cryopreservation box has multiple vent holes that are evenly distributed on the bottom plate. The cell cryopreservation box has a protective box inside, and an auxiliary cavity inside the protective box. Two freezing tubes are arranged symmetrically inside the auxiliary cavity. The bottom of the freezing tubes passes through the cell cryopreservation box and connects to the freezer. An air outlet pipe on the outside of the cell cryopreservation box passes through the protective box and connects to the exhaust pipe.

[0005] The protective box of this utility model has multiple positioning slide rails on the inside, which are symmetrically arranged. Multiple storage trays are connected to the inside of the cell cryopreservation box. The storage trays slide inside the cell cryopreservation box. The bottom of the storage tray is connected to the positioning slide rails, and the storage trays slide on the positioning slide rails. The outside of the storage tray has two fixed protrusions, which are symmetrically arranged.

[0006] The protective box of this utility model has multiple positioning grooves on its side wall, which are symmetrically arranged. The fixing protrusion is adapted to the positioning groove, and the outside of the fixing protrusion is connected to the positioning groove. The fixing protrusion slides in the positioning groove. The upper part of the storage tray has multiple positioning slots, which are evenly distributed on the upper part of the storage tray. The upper part of the storage tray has multiple nameplate sockets, which are evenly distributed on the upper part of the storage tray. The nameplate sockets are located outside the positioning slots.

[0007] The positioning slot of this invention has multiple adjusting grooves on its side wall, which are evenly distributed. A spring is connected to the inner side of each adjusting groove, and the spring slides within the groove. One end of the spring is connected to a fastening plate, and one end of the fastening plate is connected to the adjusting groove, which slides within the groove. The lower part of the fastening plate is connected to the positioning slot, and the fastening plate slides within it. The bottom of the fastening plate has an auxiliary groove, and the bottom of the positioning slot has multiple auxiliary protrusions, which are evenly distributed at the bottom. The outer side of the auxiliary groove is connected to these auxiliary protrusions, and the auxiliary groove slides within them. The outer side of the fastening plate is connected to a cell storage tube, which slides outside the fastening plate. The bottom of the cell storage tube is connected to the positioning slot. Beneficial effects

[0008] The cold air generated by this freezer cools the cell cryopreservation box from all directions along two different paths: the exhaust port and the freezing tube. This results in fast freezing speed and good freezing effect, ensuring the survival rate of cells inside the cell storage tube.

[0009] The fastening clamp of this invention slides and adjusts within the positioning slot according to the outer dimensions of the cell storage tube, enabling the device to adapt to cell storage tubes of different sizes for cryopreservation. It is convenient to store and retrieve, has a wide range of applications, is simple to operate, easy to adjust, and has a reasonable structure.

[0010] This utility model features a fastening clamp that holds the cell storage tube in place, ensuring that the cell storage tube stands vertically in the positioning slot. This effectively prevents the cell storage tube from tilting and spilling, and provides a good limiting effect. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the structure of a novel NK cell cryopreservation device according to the present invention.

[0012] Figure 2 This is a schematic diagram of the structure of a novel NK cell cryopreservation device, a cell cryopreservation box, as described in this utility model.

[0013] Figure 3 This is a schematic diagram of the storage tray structure of a novel NK cell cryopreservation device according to the present invention.

[0014] Figure 4 This is a schematic diagram of the fastening clamp and spring combination structure of a novel NK cell cryopreservation device according to the present invention.

[0015] Figure 5 This is a schematic diagram of the combination structure of the freezer 02 and the cryotubes in a novel NK cell cryopreservation device according to this utility model.

[0016] Figure 6 This is a cross-sectional view of the structure of a novel NK cell cryopreservation device according to the present invention. Detailed Implementation

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments: Example 1: A novel NK cell cryopreservation device includes a cell cryopreservation box 01, a freezer 02, and a sealing door 03. The cell cryopreservation box 01 is connected to the sealing door 03 via a hinge. The freezer 02 is connected to the bottom of the cell cryopreservation box 01. The bottom plate of the cell cryopreservation box 01 has multiple vent holes 08, which are evenly distributed on the bottom plate. The cell cryopreservation box 01 has a protective box 10 inside, and an auxiliary cavity 18 inside the protective box 10. Two freezing tubes 16 are arranged symmetrically inside the auxiliary cavity 18. The bottom of the freezing tubes 16 passes through the cell cryopreservation box 01 and connects to the freezer 02. An air outlet pipe on the outside of the cell cryopreservation box 01 passes through the protective box 10 and connects to an exhaust pipe 09. The cold air generated by the freezer 02 cools the cell cryopreservation box 01 from all directions along two different paths: the exhaust holes 08 and the freezing tubes 16. The freezing speed is fast and the freezing effect is good, ensuring the survival rate of cells inside the cell storage tubes 14.

[0018] Example 2: The protective box 10 of this utility model has multiple positioning slide rails 11 on its inner side, which are symmetrically arranged. Multiple storage trays 04 are connected to the inner side of the cell cryopreservation box 01. The storage trays 04 slide inside the cell cryopreservation box 01. The bottom of the storage trays 04 is connected to the positioning slide rails 11, and the storage trays 04 slide on the positioning slide rails 11. The outer side of the storage trays 04 has two fixing protrusions 15, which are symmetrically arranged.

[0019] Example 3: The protective box 10 of this utility model has multiple positioning grooves 13 on its side wall. The positioning grooves 13 are symmetrically arranged. The fixing protrusions 15 are adapted to the positioning grooves 13. The outer side of the fixing protrusions 15 is connected to the positioning grooves 13. The fixing protrusions 15 slide in the positioning grooves 13. The upper part of the storage tray 04 has multiple positioning slots 05. The positioning slots 05 are evenly distributed on the upper part of the storage tray 04. The upper part of the storage tray 04 has multiple nameplate sockets 07. The nameplate sockets 07 are evenly distributed on the upper part of the storage tray 04. The nameplate sockets 07 are located around the positioning slots 05.

[0020] Example 4: The positioning slot 05 of this utility model has multiple adjusting grooves 12 on its side wall, which are evenly distributed on the side wall of the positioning slot 05. A spring 17 is connected to the inner side of each adjusting groove 12, and the spring 17 slides within the adjusting groove 12. One end of the spring 17 is connected to a fastening plate 06, and one end of the fastening plate 06 is connected to the adjusting groove 12, also sliding within the adjusting groove 12. The lower part of the fastening plate 06 is connected to the positioning slot 05, and the fastening plate 06 slides within the fastening plate 06. The bottom of the fastening plate 06 has an auxiliary groove 19, and the bottom of the positioning slot 05 has multiple auxiliary protrusions 20, which are evenly distributed on the bottom of the positioning slot 05. The outer side of the auxiliary groove 19 is connected to... The auxiliary protrusion 20 is connected, and the auxiliary slide 19 slides within the auxiliary protrusion 20. The cell storage tube 14 is connected to the outside of the fastening clamp 06, and the cell storage tube 14 slides outside the fastening clamp 06. The bottom of the cell storage tube 14 is connected to the positioning slot 05. The fastening clamp 06 clamps the cell storage tube 14, ensuring that the cell storage tube 14 stands vertically in the positioning slot 05, effectively preventing the cell storage tube 14 from tilting and spilling. The limiting effect is good. The fastening clamp 06 slides and adjusts within the positioning slot 05 according to the outer dimensions of the cell storage tube 14, so that the device can adapt to cell storage tubes 14 of different sizes for cryopreservation. It is convenient to store and retrieve, has a wide range of applications, is simple to operate, easy to adjust, and has a reasonable structure.

[0021] Example 5: Installation steps: First, open the sealing door 03, pull the storage tray 04 outwards, insert the storage tray 04 into the positioning slot 05, then insert the label indicating the status of the storage tray 04 into the label socket 07, and then push the storage tray 04 back into the cell cryopreservation box 01. Then, turn on the freezer 02. The freezer 02 will start working. Part of the cold air from the freezer 02 will cool the cell cryopreservation box 01 through the exhaust port 08, while another part of the cold air from the freezer 02 will enter the freezing pipe 16 and be ejected through the exhaust pipe 09 to cool the cell cryopreservation box 01. The cold air generated by the freezer 02 will cool the cell cryopreservation box 01 through the exhaust port 08. 8. The cryotubes 16 cool the cell cryopreservation box 01 from all directions via two different paths, resulting in fast freezing speed and good freezing effect, ensuring the survival rate of cells inside the cell storage tubes 14. The fastening clamps 06 slide and adjust within the positioning slots 05 according to the outer dimensions of the cell storage tubes 14, enabling the device to adapt to cell storage tubes 14 of different sizes for cryopreservation. It is convenient to store and retrieve, has a wide range of applications, is simple to operate, easy to adjust, and has a reasonable structure. At the same time, the fastening clamps 06 clamp the cell storage tubes 14, ensuring that the cell storage tubes 14 stand vertically within the positioning slots 05, effectively preventing the cell storage tubes 14 from tilting and spilling, and providing a good limiting effect.

[0022] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. An NK cell cryopreservation device, characterized in that: The device includes a cell cryopreservation box (01), a freezer (02), and a sealing door (03). The cell cryopreservation box (01) is connected to the sealing door (03) via a hinge. The bottom of the cell cryopreservation box (01) is connected to the freezer (02). The bottom plate of the cell cryopreservation box (01) has multiple exhaust holes (08), which are evenly distributed on the bottom plate of the cell cryopreservation box (01). The cell cryopreservation box (01) has a protective box (10) inside. The protective box (10) has an auxiliary cavity (18) inside. Two freezing tubes (16) are arranged inside the auxiliary cavity (18). The freezing tubes (16) are symmetrically arranged. The bottom of the freezing tubes (16) passes through the cell cryopreservation box (01) and connects to the freezer (02). The air outlet pipe on the outside of the cell cryopreservation box (01) passes through the protective box (10) and connects to the exhaust pipe (09).

2. The NK cell cryopreservation device according to claim 1, characterized in that: The protective box (10) has multiple positioning slide rails (11) on its inner side, which are symmetrically arranged. The cell cryopreservation box (01) is connected to multiple storage trays (04) on its inner side. The storage trays (04) slide inside the cell cryopreservation box (01). The bottom of the storage trays (04) is connected to the positioning slide rails (11), which slide on the positioning slide rails (11). The storage trays (04) have two fixed protrusions (15) on their outer side, which are symmetrically arranged.

3. The NK cell cryopreservation device according to claim 2, characterized in that: The protective box (10) has multiple positioning grooves (13) on its side wall. The positioning grooves (13) are symmetrically arranged. The fixed protrusion (15) is adapted to the positioning groove (13). The fixed protrusion (15) is connected to the positioning groove (13) on its outer side. The fixed protrusion (15) slides in the positioning groove (13). The upper part of the storage tray (04) has multiple positioning slots (05). The positioning slots (05) are evenly distributed on the upper part of the storage tray (04). The upper part of the storage tray (04) has multiple nameplate sockets (07). The nameplate sockets (07) are evenly distributed on the upper part of the storage tray (04). The nameplate sockets (07) are located around the positioning slots (05).

4. The NK cell cryopreservation device according to claim 3, characterized in that: The positioning slot (05) has multiple adjusting grooves (12) on its side wall. The adjusting grooves (12) are evenly distributed on the side wall of the positioning slot (05). A spring (17) is connected to the inside of the adjusting groove (12). The spring (17) slides in the adjusting groove (12). One end of the spring (17) is connected to a fastening plate (06). One end of the fastening plate (06) is connected to the adjusting groove (12). The fastening plate (06) slides in the adjusting groove (12). The lower part of the fastening plate (06) is connected to the positioning slot (05). The fastening plate (06) slides in the fastening plate (06).

5. The NK cell cryopreservation device according to claim 3, characterized in that: The fastening clamp (06) has an auxiliary groove (19) at the bottom, and the positioning slot (05) has multiple auxiliary protrusions (20) at the bottom. The auxiliary protrusions (20) are evenly distributed at the bottom of the positioning slot (05). The auxiliary groove (19) is connected to the auxiliary protrusions (20) on the outside. The auxiliary groove (19) slides in the auxiliary protrusions (20). The fastening clamp (06) is connected to the cell storage tube (14) on the outside. The cell storage tube (14) slides on the outside of the fastening clamp (06). The bottom of the cell storage tube (14) is connected to the positioning slot (05).

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