A cell cryopreservation solution filtration device

Abstract

The utility model relates to filter technical field especially relates to a cell cryopreservation solution filtering device. Its technical scheme includes flow restriction mechanism, flow restriction mechanism includes flow guide cylinder, the bottom fixed mounting of flow guide cylinder has filter membrane, the bottom of filter membrane is elastically connected with the bearing spring between prestorage cylinder, the inside slide connection of prestorage cylinder has flow limiting plate, bearing spring is provided with transmission between circular ring, the outer wall of flow guide cylinder is connected with prestorage cylinder slidingly, flow limiting plate is located the top of filter membrane. In the utility model, because the filtering speed of circular ring is limited, a large number of cell cryopreservation solution is pressed on circular ring, and the filtering pressure rises suddenly, at this moment, the overall weight of flow guide cylinder and filter membrane increases, so that the flow guide cylinder and filter membrane move downwards, the flow guide cylinder makes flow limiting plate slide by circular ring and connecting rod, thereby reducing the opening size of flow guide port, that is, the adding speed of cell cryopreservation solution can be reduced, the filtering pressure is avoided to rise suddenly, and filter membrane is broken, liquid leakage is avoided.

Description

Technical Field

[0001] This utility model relates to the field of filtration technology, and in particular to a cell cryopreservation solution filtration device. Background Technology

[0002] Cell cryopreservation solution is a key reagent in the cell cryopreservation process. It primarily works through the synergistic effect of multiple components to protect cell viability at low temperatures, preventing cell damage or death and ensuring long-term cell preservation for subsequent research and applications. Cryopreservation solution is filtered to remove impurities, microorganisms, and other substances that may affect cell viability, ensuring the purity and safety of the cryopreservation solution.

[0003] The rotary stirring filter uses a rotary motor to drive a rotating rod, stirring plate, and first filter screen to rotate. In conjunction with the second filter screen, it stirs and performs initial filtration of the added cell cryopreservation solution. Then, a pump and a suction pipe are used to extract the cell cryopreservation solution after the initial filtration into the second filter box for secondary filtration.

[0004] However, if the rate at which the cell cryopreservation solution is added is not carefully monitored, rapid pouring can cause the filter (such as a 0.22μm sterile filter membrane) to become instantly clogged, leading to a sudden increase in filtration pressure, or even membrane rupture and liquid leakage. Utility Model Content

[0005] The purpose of this invention is to address the problem in the prior art where the rapid addition of cell cryopreservation solution causes a sudden increase in filtration pressure, leading to membrane rupture and liquid leakage. This invention proposes a cell cryopreservation solution filtration device.

[0006] The technical solution of this utility model: A cell cryopreservation solution filtration device, comprising a base, a stirring cylinder fixedly mounted on the top of the base, stirring blades rotatably connected inside the stirring cylinder, a pre-storage cylinder fixedly mounted on the top of the stirring cylinder, and further comprising:

[0007] A flow limiting mechanism includes a flow guide tube, a filter membrane fixedly installed at the bottom of the flow guide tube, a load-bearing spring elastically connected between the bottom of the filter membrane and a pre-storage tube, a flow limiting plate slidably connected inside the pre-storage tube, and a transmission component provided between the load-bearing spring and the ring.

[0008] The outer wall of the flow guide tube is slidably connected to the pre-storage tube, the flow limiting plate is located above the filter membrane, and an elastic holding mechanism is provided inside the pre-storage tube and below the flow guide tube.

[0009] Optionally, the transmission component includes a ring, and the top of the guide tube is fixedly mounted with the ring. The ring slides up and down inside the pre-storage tube. A connecting rod is hinged to the inner wall of the ring, and the other end of the connecting rod is hinged to the flow limiting plate.

[0010] Optionally, the inner wall of the ring is covered with a sealing rubber layer, and a vertical groove is formed between the connecting rod and the interlayer.

[0011] Optionally, the guide tube adopts a bowl-shaped structure, the pre-storage tube has a guide port inside, and the flow limiting plate slides at the guide port.

[0012] Optionally, the elastic maintaining mechanism includes a seepage-proof ring, with the seepage-proof ring fixedly installed at the bottom of the filter membrane, and the filter pores on the filter membrane located inside the seepage-proof ring.

[0013] Optionally, a limiting ring is fixedly installed inside the pre-storage cylinder, and the limiting ring is located on the sliding path of the guide cylinder.

[0014] Optionally, the pre-storage cylinder has an anti-seepage cavity inside, and the ring slides up and down inside the anti-seepage cavity.

[0015] Optionally, a motor is fixedly installed inside the base, and the drive shaft of the motor is fixedly connected to the stirring blade.

[0016] Compared with the prior art, the present invention has the following beneficial technical effects:

[0017] In this invention, due to the limited filtration speed of the circular ring, a large amount of cell cryopreservation fluid is pressed onto the circular ring, causing a sudden increase in filtration pressure. At this time, the overall weight of the guide tube and filter membrane increases, causing the guide tube and filter membrane to move downward. The guide tube uses the circular ring and connecting rod to make the flow limiting plate slide, thereby reducing the size of the guide port opening. This reduces the rate at which the cell cryopreservation fluid is added, preventing a sudden increase in filtration pressure that could lead to filter membrane rupture and liquid leakage.

[0018] Furthermore, the limiting ring restricts the movement range of the guide tube to prevent it from moving too far, which would cause the load-bearing spring to be over-compressed and lose its original elasticity. The anti-seepage ring guides the cell cryopreservation solution to prevent it from flowing to the load-bearing spring, which would corrode the spring and affect the volume of the intercepted cell cryopreservation solution. Attached Figure Description

[0019] Figure 1 A schematic diagram of the overall structure of this utility model is provided;

[0020] Figure 2 This is a cross-sectional view of the stirring tank structure;

[0021] Figure 3 This is a cross-sectional view of the pre-storage cylinder structure;

[0022] Figure 4 This is a schematic diagram of the stirring blade structure.

[0023] Reference numerals in the attached drawings: 1. Base; 2. Stirring drum; 3. Stirring blade; 4. Pre-storage drum; 5. Flow limiting mechanism; 51. Guide tube; 52. Filter membrane; 53. Load-bearing spring; 54. Ring; 55. Connecting rod; 56. Flow limiting plate; 57. Flow guide port; 6. Elastic maintaining mechanism; 61. Anti-seepage ring; 62. Limiting ring; 63. Anti-seepage cavity. Detailed Implementation

[0024] The technical solution of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of this utility model, but not all embodiments.

[0025] The components of the present invention embodiments described and shown in the accompanying drawings can typically be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention.

[0026] Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0027] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0028] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0029] Example 1

[0030] This embodiment proposes a cell cryopreservation solution filtration device, such as... Figure 1 and Figure 4As shown, it includes a base 1, and a stirring cylinder 2 is fixedly installed on the top of the base 1. A stirring blade 3 is rotatably connected inside the stirring cylinder 2. The stirring blade 3 stirs the filtered cell cryopreservation solution inside the stirring cylinder 2 to prevent precipitation. A pre-storage cylinder 4 is fixedly installed on the top of the stirring cylinder 2.

[0031] like Figure 2 and Figure 3 As shown, the pre-storage cylinder 4 is equipped with a flow limiting mechanism 5, which includes a flow guide cylinder 51. The flow guide cylinder 51 has a bowl-shaped structure, and its outer wall is slidably connected to the pre-storage cylinder 4. A filter membrane 52 is fixedly installed at the bottom of the flow guide cylinder 51, and a load-bearing spring 53 is elastically connected between the bottom of the filter membrane 52 and the pre-storage cylinder 4. Due to the limited flow rate of the cell cryopreservation solution at the filter screen, cell cryopreservation solution will remain at the flow guide cylinder 51 and the filter membrane 52. The weight of the cell cryopreservation solution and the flow guide cylinder 51 and the filter membrane 52 presses against the load-bearing spring 53, causing the flow guide cylinder 51 and the filter membrane 52 to slide downwards.

[0032] The pre-storage cylinder 4 has a flow-limiting plate 56 slidably connected inside, and the flow-limiting plate 56 is located above the filter membrane 52. The pre-storage cylinder 4 has a flow guide port 57 inside, and the flow-limiting plate 56 slides at the flow guide port 57. A transmission component is provided between the load-bearing spring 53 and the ring 54. The transmission component includes the ring 54. The top of the flow guide cylinder 51 is fixedly installed with the ring 54. The ring 54 slides up and down inside the pre-storage cylinder 4. A connecting rod 55 is hinged to the inner wall of the ring 54. The other end of the connecting rod 55 is hinged to the flow-limiting plate 56.

[0033] The cell cryopreservation solution stored in the flow guide tube 51 and filter membrane 52 will press down the flow guide tube 51 and filter membrane 52. The flow guide tube 51 uses the ring 54 and connecting rod 55 to make the flow limiting plate 56 slide, thereby reducing the size of the flow guide port 57, which can reduce the rate of cell cryopreservation solution addition.

[0034] In this embodiment, due to the limited filtration speed of the ring 54, a large amount of cell cryopreservation fluid is pressed onto the ring 54, causing a sudden increase in filtration pressure. At this time, the overall weight of the guide tube 51 and the filter membrane 52 increases, causing the guide tube 51 and the filter membrane 52 to move downward. The guide tube 51 uses the ring 54 and the connecting rod 55 to make the flow limiting plate 56 slide, thereby reducing the size of the opening of the guide port 57. This reduces the rate at which the cell cryopreservation fluid is added, preventing a sudden increase in filtration pressure that could lead to filter membrane rupture and liquid leakage.

[0035] Example 2

[0036] Based on Example 1, this example proposes a cell cryopreservation solution filtration device, such as... Figure 2 and Figure 3As shown, an elastic holding mechanism 6 is provided inside the pre-storage cylinder 4 and below the guide cylinder 51. The elastic holding mechanism 6 includes a seepage-proof ring 61. The seepage-proof ring 61 is fixedly installed at the bottom of the filter membrane 52, and the filter holes on the filter membrane 52 are located inside the seepage-proof ring 61. The seepage-proof ring 61 prevents the cell cryopreservation solution from flowing to the load-bearing spring 53, which would cause the load-bearing spring 53 to be corroded.

[0037] A limiting ring 62 is fixedly installed inside the pre-storage cylinder 4, and the limiting ring 62 is located on the sliding path of the guide cylinder 51. The limiting ring 62 restricts the movement range of the guide cylinder 51 to prevent the guide cylinder 51 from moving too far, which would cause the load-bearing spring 53 to be over-compressed and lose its original elasticity.

[0038] The pre-storage cylinder 4 has an internal anti-seepage cavity 63. The ring 54 slides up and down within the anti-seepage cavity 63. The inner wall of the ring 54 is lined with a sealing rubber layer. A vertical groove is formed between the connecting rod 55 and the interlayer. The sealing rubber layer and the anti-seepage cavity 63 are used to prevent the cell cryopreservation solution from seeping in.

[0039] In this embodiment, the anti-seepage ring 61 is used to guide the cell cryopreservation solution to prevent the cell cryopreservation solution from flowing to the load-bearing spring 53, which would cause the load-bearing spring 53 to be corroded. At the same time, the limiting ring 62 restricts the movement range of the guide tube 51 to avoid the guide tube 51 moving too far, which would cause the load-bearing spring 53 to be over-compressed, causing the load-bearing spring 53 to lose its original elasticity and affect the implementation of Embodiment 1.

[0040] The above specific embodiments are merely several optional embodiments of this utility model. Based on the technical solution of this utility model and the relevant teachings of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A cell cryopreservation solution filtration device, comprising a base (1), a stirring cylinder (2) fixedly mounted on the top of the base (1), a stirring blade (3) rotatably connected inside the stirring cylinder (2), and a pre-storage cylinder (4) fixedly mounted on the top of the stirring cylinder (2), characterized in that, Also includes: The flow limiting mechanism (5) includes a flow guide tube (51), a filter membrane (52) is fixedly installed at the bottom of the flow guide tube (51), a load-bearing spring (53) is elastically connected between the bottom of the filter membrane (52) and the pre-storage cylinder (4), a flow limiting plate (56) is slidably connected inside the pre-storage cylinder (4), and a transmission component is provided between the load-bearing spring (53) and the ring (54). The outer wall of the flow guide tube (51) is slidably connected to the pre-storage tube (4), the flow limiting plate (56) is located above the filter membrane (52), and an elastic holding mechanism (6) is provided inside the pre-storage tube (4) and below the flow guide tube (51).

2. The cell cryopreservation solution filtration device according to claim 1, characterized in that: The transmission component includes a ring (54), and the top of the flow guide (51) is fixedly installed with the ring (54). The ring (54) slides up and down inside the pre-storage cylinder (4). The inner wall of the ring (54) is hinged with a connecting rod (55), and the other end of the connecting rod (55) is hinged to the flow limiting plate (56).

3. The cell cryopreservation solution filtration device according to claim 2, characterized in that: The inner wall of the ring (54) is covered with a sealing rubber layer, and a vertical groove is provided between the connecting rod (55) and the interlayer.

4. The cell cryopreservation solution filtration device according to claim 3, characterized in that: The guide tube (51) adopts a bowl-shaped structure, and the pre-storage tube (4) has a guide port (57) inside, and the flow limiting plate (56) slides at the guide port (57).

5. The cell cryopreservation solution filtration device according to claim 4, characterized in that: The elastic maintaining mechanism (6) includes a seepage-proof ring (61), and the bottom of the filter membrane (52) is fixedly installed with the seepage-proof ring (61), and the filter holes on the filter membrane (52) are located inside the seepage-proof ring (61).

6. The cell cryopreservation solution filtration device according to claim 5, characterized in that: A limiting ring (62) is fixedly installed inside the pre-storage cylinder (4), and the limiting ring (62) is located on the sliding path of the guide cylinder (51).

7. The cell cryopreservation solution filtration device according to claim 6, characterized in that: The pre-storage cylinder (4) has an anti-seepage cavity (63) inside, and the ring (54) slides up and down inside the anti-seepage cavity (63).

8. The cell cryopreservation solution filtration device according to claim 1, characterized in that: A motor is fixedly installed inside the base (1), and the drive shaft of the motor is fixedly connected to the stirring blade (3).

Images