Anti-clogging liquid chromatography system

Abstract

The utility model discloses a kind of anti-blocking liquid chromatography systems, belong to liquid chromatography field, including the sampling flow channel connected with sample injector, for sucking sample;Extraction flow channel connected with blocking column, first chromatographic column and second chromatographic column, for conveying the first mobile phase after impurity removal of blocker grid sample, sample is conveyed to first chromatographic column and second chromatographic column and carries out the separation of effective component;Analysis flow channel connected with third chromatographic column;For separating and detecting captured substance;First multi-way valve, and selectively switched into first state and second state;Second multi-way valve, and selectively switched into third state and fourth state;Through the state switching cooperation of first multi-way valve and second multi-way valve, realize the sampling, extraction, analysis, cleaning function of liquid chromatography system, the utility model can effectively avoid chromatographic column absorb impurity particles in mobile phase and high retention material, greatly prolong the service life of chromatographic column.

Description

Technical Field

[0001] This utility model relates to the field of liquid chromatography analysis instruments, specifically to an anti-clogging liquid chromatography system. Background Technology

[0002] A liquid chromatograph, as a system for chromatographic separation and analysis, includes an injection system, a detection system, a recording and data processing system, a temperature control system, and a mobile phase control system. Among these, the injection system is the first unit in liquid chromatographic analysis. Traditional injection processes have the following problems: 1. The mobile phase may contain many particles and impurities. Because the chromatographic column is very precise, these impurities can easily cause blockage when they enter the column with the mobile phase, affecting the analytical results and damaging the column. 2. Existing technologies typically add a barrier capsule between the extraction column and the analysis column. However, since the barrier capsule does not have regeneration capabilities and expands after being exposed to blood and wastewater, its volume will continue to increase, causing blockage of the pipeline.

[0003] Patent No. CN200610134027.5 discloses a two-dimensional high-performance liquid chromatography (HPLC) system and its application, consisting of five high-pressure liquid chromatography solvent delivery pumps, a solvent mixer, a ten-way valve, a six-way valve, two HPLC analytical columns respectively suitable for separating hydrophobic and hydrophilic components, and a component transfer column. During application, the chromatographic columns are highly susceptible to blockage and damage due to matrix effects that accumulate impurities and highly retained substances, requiring frequent column replacements and incurring high economic costs.

[0004] Therefore, it is necessary to design a liquid chromatography system that ensures the quality of liquid analysis and prevents clogging, in order to solve the problems existing in the traditional sample introduction process. Utility Model Content

[0005] In view of the shortcomings of the existing technology, the purpose of this utility model is to solve the problems in existing liquid chromatography systems, such as impurities easily entering the system with the mobile phase and affecting the analytical structure, easily damaging the chromatographic column, and easy clogging of the filter cartridge.

[0006] The technical solution of this utility model is: This invention provides an anti-clogging liquid chromatography system, comprising: a first multi-port valve with multiple interfaces, a second multi-port valve with multiple ports, and a waste liquid channel connected to the second multi-port valve; further comprising: a first connecting pipe sequentially connected to a first mobile phase, a first delivery pump, and a barrier column, for delivering the first mobile phase, the first connecting pipe being connected to one interface of the first multi-port valve; a second connecting pipe connected to an injector for aspirating a sample solution, the second connecting pipe being connected to the first connecting pipe via a tee I, with the barrier column located upstream of the tee I; and a third connecting pipe connected to a second mobile phase and a second delivery pump, for delivering the second mobile phase... Two mobile phases; a third connecting line connected to one port of the first multi-port valve; a fourth connecting line connected to the first chromatographic column, with its two ends connected to different ports of the second multi-port valve; a fifth connecting line connected to the second chromatographic column, with its two ends connected to different ports of the second multi-port valve; a sixth connecting line connected to the third chromatographic column and the detector, with its six ends connected to one port of the first multi-port valve; and a seventh, eighth, and ninth connecting lines connected between the first and second multi-port valves; and a tenth connecting line connected between the two ports of the second multi-port valve.

[0007] Preferably, the first multi-way valve includes interface a, interface b, interface c, interface d, interface e, and interface f, and the second multi-way valve includes interface g, interface h, interface i, interface j, interface k, interface l, interface m, interface n, interface o, and interface p.

[0008] Preferably, one end of the first connecting pipe is connected to the first delivery pump, and the other end is connected to interface c; one end of the second connecting pipe is connected to the injector, and the other end is connected to the tee I; one end of the third connecting pipe is connected to the second delivery pump, and the other end is connected to interface a; one end of the fourth connecting pipe is connected to interface k, and the other end is connected to interface n; one end of the fifth connecting pipe is connected to interface i, and the other end is connected to interface P; one end of the sixth connecting pipe is connected to the detector, and the other end is connected to interface f; one end of the seventh connecting pipe is connected to interface b, and the other end is connected to interface g; one end of the eighth connecting pipe is connected to interface e, and the other end is connected to interface h; one end of the ninth connecting pipe is connected to interface d, and the other end is connected to interface m; one end of the tenth connecting pipe is connected to interface j, and the other end is connected to interface i.

[0009] Preferably, it also includes a waste liquid end, and the interface o is connected to the waste liquid end.

[0010] Preferably, the first multi-way valve includes a first state and a second state; when the first multi-way valve is in the first state, interface b is connected to interface c, interface d is connected to interface e, and interface a is connected to interface f; when the first multi-way valve is in the second state, interface a is connected to interface b, interface c is connected to interface d, and interface e is connected to interface f.

[0011] Preferably, the second multi-way valve includes a third state and a fourth state; when the second multi-way valve is in the third state, interface g is connected to interface h, interface i is connected to interface j, interface k is connected to interface l, interface m is connected to interface n, and interface o is connected to interface p; when the second multi-way valve is in the fourth state, interface g is connected to interface p, interface h is connected to interface i, interface j is connected to interface k, interface l is connected to interface m, and interface n is connected to interface o.

[0012] Preferably, it further includes a twelfth connecting line connected to the conditioning solution and the third delivery pump, the twelfth connecting line being connected to the first connecting line via a tee II.

[0013] Compared with the prior art, the beneficial effects of this utility model are: 1. This utility model adds a barrier column at the front end of the extraction flow to prevent the chromatographic column from absorbing particles and highly retained substances in the sample due to enrichment, which would affect the analytical detection results and service life of the chromatographic column.

[0014] This invention can automatically reverse clean the first and second chromatographic columns, including both individual and parallel cleaning modes. It can regenerate the chromatographic columns, greatly enhancing their service life and reducing operating costs.

[0015] The detailed structure of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present invention; Figure 2 This is a schematic diagram of the extraction function operation status in Embodiment 1 of this utility model; Figure 3 This is a schematic diagram of the operation status of the analysis function in Embodiment 1 of this utility model; Figure 4 This is a schematic diagram of the parallel cleaning function operation status in Embodiment 1 of this utility model; Figure 5 This is a schematic diagram of the operation status of the individual cleaning function in Embodiment 1 of this utility model; Figure 6 This is a schematic diagram showing the parallel operation of the modulation and extraction functions in Embodiment 2 of this utility model; Component names and corresponding serial numbers: 0. Interface a; 1. Interface b; 2. Interface c; 3. Interface d; 4. Interface e; 5. Interface f; 6. Interface g; 7. Interface h; 8. Interface i; 9. Interface j; 10. Interface k; 11. Interface l; 12. Interface m; 13. Interface n; 14. Interface o; 15. Interface p; S1, First mobile phase; S2, Second mobile phase; S3, Preparation solution; S4, Washing solution; P1, First delivery pump; P2, Second delivery pump; P3, Third delivery pump; AS, Injector; SIL, Barrier column; F, Waste end; T1, T-connector I; T2, T-connector II; C1, First column; C2, Second column; C3, Third column; DE, Detector; V1, First multi-port valve; V2, Second multi-port valve; L1, First connecting line; L2, Second connecting line; L3, Third connecting line; L4, Fourth connecting line; L5, Fifth connecting line; L6, Sixth connecting line; L7, Seventh connecting line; L8, Eighth connecting line; L9, Ninth connecting line; L10, Tenth connecting line; L11, Eleventh connecting line; L12, Twelfth connecting line. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can be arranged and designed in various different configurations. The following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely to illustrate selected embodiments of the utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0018] Unless otherwise defined, the technical or scientific terms used in this disclosure shall have the ordinary meaning understood by a person skilled in the art to which this disclosure pertains. The words “comprising” or “including” and similar terms used in this disclosure mean that the element or object preceding the word covers the element or object listed after the word and its equivalents, without excluding other elements or objects. The words “connected” or “linked” and similar terms are not limited to physical or mechanical connections, but may also include electrical connections, whether direct or indirect. “Up,” “down,” “left,” “right,” etc., are used only to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly. Example 1

[0019] Please see Figure 1 This invention provides an anti-clogging liquid chromatography system, comprising: a first multi-port valve V1 with multiple interfaces, a second multi-port valve V2 with multiple ports, and a waste liquid channel L11 connected to the second multi-port valve V2; further comprising: a first connecting pipe L1 connected in sequence to a first mobile phase S1, a first delivery pump P1, and a barrier column S11 for delivering the first mobile phase, the first connecting pipe L1 being connected to one interface of the first multi-port valve V1; a second connecting pipe L2 connected to an injector AS for drawing sample solution A1, the second connecting pipe L2 being connected to the first connecting pipe L1 via a tee IT1, and the barrier column S11 being located upstream of the tee IT1; and a third connecting pipe L3 connected to the second mobile phase S2 and the second delivery pump P2 for delivering the second mobile phase S1. The mobile phase S2 is connected to a third connecting line L3, which is connected to one port of the first multi-port valve V1; a fourth connecting line L4 is connected to the first chromatographic column C1, with its two ends connected to different ports of the second multi-port valve V2; a fifth connecting line L5 is connected to the second chromatographic column C2, with its two ends connected to different ports of the second multi-port valve V2; a sixth connecting line L6 is connected to the third chromatographic column C3 and the detector DE, with its six ends connected to one port of the first multi-port valve V1; and a seventh connecting line L7, an eighth connecting line L8, and a ninth connecting line L9 are connected between the first multi-port valve V1 and the second multi-port valve V2; and a tenth connecting line L10 is connected between the two ports of the second multi-port valve V2.

[0020] Specifically, the first multi-way valve V1 is a six-way valve, and the second multi-way valve V2 is a ten-way valve. The first multi-way valve V1 includes interface a0, interface b1, interface c2, interface d3, interface e4, and interface f5, and the second multi-way valve V2 includes interface g6, interface h7, interface i8, interface j9, interface k10, interface l11, interface m12, interface n13, interface o14, and interface p15.

[0021] Specifically, one end of the first connecting pipe L1 is connected to the first delivery pump P1, and the other end is connected to interface c2; one end of the second connecting pipe L2 is connected to the injector AS, and the other end is connected to the tee IT1; one end of the third connecting pipe L3 is connected to the second delivery pump P2, and the other end is connected to interface a0; one end of the fourth connecting pipe L4 is connected to interface k10, and the other end is connected to interface n13; one end of the fifth connecting pipe L5 is connected to interface i8, and the other end is connected to interface P15; one end of the sixth connecting pipe L6 is connected to detector DE, and the other end is connected to interface f5; one end of the seventh connecting pipe L7 is connected to interface b1, and the other end is connected to interface g6; one end of the eighth connecting pipe L8 is connected to interface e4, and the other end is connected to interface h7; one end of the ninth connecting pipe L9 is connected to interface d3, and the other end is connected to interface m12; one end of the tenth connecting pipe L10 is connected to interface j9, and the other end is connected to interface i11.

[0022] Specifically, it also includes a waste liquid end F, and the interface o14 is connected to the waste liquid end F.

[0023] Specifically, the first multi-way valve V1 includes a first state and a second state; when the first multi-way valve V1 is in the first state, interface b1 is connected to interface c2, interface d3 is connected to interface e4, and interface a0 is connected to interface f5; when the first multi-way valve V1 is in the second state, interface a0 is connected to interface b1, interface c2 is connected to interface d3, and interface e4 is connected to interface f5.

[0024] Specifically, the second multi-way valve V2 includes a third state and a fourth state; when the second multi-way valve V2 is in the third state, interface g6 is connected to interface h7, interface i8 is connected to interface j9, interface k10 is connected to interface l11, interface m12 is connected to interface n13, and interface o14 is connected to interface p15; when the second multi-way valve V2 is in the fourth state, interface g6 is connected to interface p15, interface h7 is connected to interface i8, interface j9 is connected to interface k10, interface l11 is connected to interface m12, and interface n13 is connected to interface o14.

[0025] Function Description: Please see Figure 2Extraction function: The first delivery pump P1 is turned on, the first multi-way valve V1 is switched to the first state, and the second multi-way valve V2 is switched to the third state. The first connecting pipe L1, the seventh connecting pipe L7, the eighth connecting pipe L8, the ninth connecting pipe L9, the fourth connecting pipe L4, the tenth connecting pipe L10, the fifth connecting pipe L5, and the eleventh connecting pipe L11 are connected in sequence to form an extraction channel. The sample is drawn by the injector AS, passes through the second connecting pipe L2, and enters the first connecting pipe L1. The first mobile phase S1 mixes with the sample after passing through the barrier column SIL, and then passes through the first chromatographic column C1 and the second chromatographic column C2 in sequence. The components in the sample begin to separate under the chromatographic separation mechanism of the first mobile phase S1 and the first chromatographic column C1 and the second chromatographic column C2. The first mobile phase S1 that passes through the first chromatographic column C1 and the second chromatographic column C2 is discharged to the waste liquid end F through the eleventh connecting pipe L11, while the components to be detected in the sample are retained in the second chromatographic column C2. Please see Figure 3 Analysis function: The second transfer pump P2 is turned on, the first multi-way valve V1 is switched to the second state, connecting the third connecting pipe L3 with the seventh connecting pipe L7, and the sixth connecting pipe L6 with the eighth connecting pipe L8. The second multi-way valve V2 is switched to the fourth state, connecting the seventh connecting pipe L7 with the fifth connecting pipe L5, and the fifth connecting pipe L5 with the eighth connecting pipe L8, forming an analytical flow channel. The second mobile phase S2 is drawn in by the second transfer pump P2, and the second mobile phase S2 pushes the analyte remaining in the second chromatographic column C2 in the extraction function to the third chromatographic column C3. After the analyte is captured and analyzed in the third chromatographic column C3, it enters the detector DE for analysis. Please see Figure 4 Parallel cleaning: Replace the first mobile phase S1 with the cleaning solution S4, turn on the first delivery pump P1, switch the first multi-way valve V1 to the first state, connecting the first connecting pipe L1 and the seventh connecting pipe L7, the eighth connecting pipe L8 and the ninth connecting pipe L9, switch the second multi-way valve V2 to the fourth state, connecting the seventh connecting pipe and the fifth connecting pipe L5, the fifth connecting pipe L5 and the eighth connecting pipe L8, the ninth connecting pipe L9 and the tenth connecting pipe L10, the tenth connecting pipe L10 and the fourth connecting pipe L4, the fourth connecting pipe L4 and the eleventh connecting pipe L11. The cleaning solution S4, driven by the first delivery pump P1, sequentially flushes the second chromatographic column C2 and the first chromatographic column C1, and finally flows to the waste liquid end F. Please see Figure 5Separate cleaning: Replace the first mobile phase S1 with the cleaning solution S4, turn on the first delivery pump P1, switch the first multi-way valve V1 to the second state, connecting the first connecting pipe L1 and the ninth connecting pipe L9, switch the second multi-way valve V2 to the fourth state, connecting the ninth connecting pipe L9 and the tenth connecting pipe L10, the tenth connecting pipe L10 and the fourth connecting pipe L4, and the fourth connecting pipe L4 and the eleventh connecting pipe L11. The cleaning solution S4 is flushed by the first delivery pump P1 and finally flows to the waste liquid end F. Example 2

[0026] A clogging-resistant liquid chromatography system, based on Example 1, further includes a twelfth connecting pipe L12 connected to a modulation solution S3 and a third delivery pump P3. The twelfth connecting pipe L12 is connected to the first connecting pipe L1 via a tee II T2 and is connected upstream of the blocker SIL.

[0027] Function Description: Please see Figure 6 Modulation function: When the extraction function is running, the third delivery pump P3 can be turned on at the same time to send the modulation solution S3 through the twelfth connecting pipe L12 to the first connecting pipe L1 to mix with the first mobile phase S1. Both are then removed by the barrier SIL grid sample. By changing the pH value, ionic strength or organic phase ratio of the first mobile phase S1 through the modulation solution S3, the retention capacity of the target component on the first chromatographic column C1 and the second chromatographic column C2 is enhanced, preventing the target component from flowing out when the extraction and transfer functions are running.

[0028] The above are specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in this utility model, based on the technical solution and concept of this utility model, should be covered within the protection scope of the claims of this utility model.

Claims

1. An anti-clogging liquid chromatography system, comprising: The system comprises a first multi-way valve (V1) with multiple interfaces, a second multi-way valve (V2) with multiple ports, and a waste liquid flow channel (L11) connected to the second multi-way valve (V2); characterized in that it further comprises: A first connecting pipe (L1) is sequentially connected to a first mobile phase (S1), a first delivery pump (P1), and a barrier post (SIL) for conveying the first mobile phase. The first connecting pipe (L1) is connected to one port of a first multi-way valve (V1). A second connecting line (L2) connected to an injector (AS) is used to draw up the sample solution (A1). The second connecting line (L2) is connected to the first connecting line (L1) via a tee I (T1), and the barrier post (SIL) is located upstream of the tee I (T1). A third connecting line (L3) is connected to the second mobile phase (S2) and the second delivery pump (P2) for conveying the second mobile phase (S2). The third connecting line (L3) is connected to one port of the first multi-way valve (V1). A fourth connecting line (L4) is connected to the first chromatographic column (C1), and the two ends of the fourth connecting line (L4) are respectively connected to different ports of the second multi-port valve (V2); The fifth connecting line (L5) is connected to the second chromatographic column (C2), and the two ends of the fifth connecting line (L5) are respectively connected to different ports of the second multi-port valve (V2); A sixth connecting line (L6) is connected to the third chromatographic column (C3) and the detector (DE), and the sixth connecting line (L6) is connected to one port of the first multi-way valve (V1); And a seventh connecting pipe (L7), an eighth connecting pipe (L8) and a ninth connecting pipe (L9) connecting between the first multi-way valve (V1) and the second multi-way valve (V2); and a tenth connecting pipe (L10) connecting between the two ports of the second multi-way valve (V2).

2. The anti-clogging liquid chromatography system according to claim 1, characterized in that, The first multi-way valve (V1) includes interface a (0), interface b (1), interface c (2), interface d (3), interface e (4), and interface f (5). The second multi-way valve (V2) includes interface g (6), interface h (7), interface i (8), interface j (9), interface k (10), interface l (11), interface m (12), interface n (13), interface o (14), and interface p (15).

3. The anti-clogging liquid chromatography system according to claim 2, characterized in that: One end of the first connecting pipe (L1) is connected to the first delivery pump (P1), and the other end is connected to the interface c (2); One end of the second connecting pipe (L2) is connected to the injector (AS), and the other end is connected to the tee I (T1); One end of the third connecting pipe (L3) is connected to the second delivery pump (P2), and the other end is connected to interface a (0); One end of the fourth connecting pipe (L4) is connected to interface k (10), and the other end is connected to interface n (13); One end of the fifth connecting pipe (L5) is connected to interface i (8), and the other end is connected to interface p (15); One end of the sixth connecting pipe (L6) is connected to the detector (DE), and the other end is connected to the interface f (5); One end of the seventh connecting pipe (L7) is connected to interface b (1), and the other end is connected to interface g (6); One end of the eighth connecting pipe (L8) is connected to interface e (4), and the other end is connected to interface h (7); One end of the ninth connecting pipe (L9) is connected to interface d (3), and the other end is connected to interface m (12); One end of the tenth connecting pipe (L10) is connected to interface j (9), and the other end is connected to interface l (11).

4. A non-clogging liquid chromatography system according to claim 2 or 3, characterized in that: It also includes a waste liquid end (F), and the interface o (14) is connected to the waste liquid end (F).

5. The anti-clogging liquid chromatography system according to claim 4, characterized in that: The first multi-way valve (V1) includes a first state and a second state; when the first multi-way valve (V1) is in the first state, the interface b (1) is connected to the interface c (2), the interface d (3) is connected to the interface e (4), and the interface a (0) is connected to the interface f (5); when the first multi-way valve (V1) is in the second state, the interface a (0) is connected to the interface b (1), the interface c (2) is connected to the interface d (3), and the interface e (4) is connected to the interface f (5).

6. The anti-clogging liquid chromatography system according to claim 4, characterized in that: The second multi-way valve (V2) includes a third state and a fourth state; when the second multi-way valve (V2) is in the third state, interface g (6) is connected to interface h (7), interface i (8) is connected to interface j (9), interface k (10) is connected to interface l (11), interface m (12) is connected to interface n (13), and interface o (14) is connected to interface p (15); when the second multi-way valve (V2) is in the fourth state, interface g (6) is connected to interface p (15), interface h (7) is connected to interface i (8), interface j (9) is connected to interface k (10), interface l (11) is connected to interface m (12), and interface n (13) is connected to interface o (14).

7. A non-clogging liquid chromatography system according to any one of claims 1, 2, 3, 5, and 6, characterized in that: It also includes a twelfth connecting line (L12) that connects the conditioning solution (S3) and the third delivery pump (P3), and the twelfth connecting line (L12) is connected to the first connecting line (L1) via a tee II (T2).

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