A device for rapid inoculation of cotton plants with kanamycin

Abstract

This utility model discloses a device for rapid kanamycin inoculation of cotton plants, comprising a handle, a telescopic tube, and an inoculation head arranged sequentially from left to right. The left end of the handle is a bacterial solution storage chamber, and the right end is an equipment chamber. The equipment chamber contains a telescopic tube reel. One end of the telescopic tube reel passes through the telescopic tube and is connected to the inoculation head. The other end of the telescopic tube reel is connected to the bacterial solution storage chamber via a valve and a diaphragm pump. This invention relates to the field of cotton breeding technology. It allows for precise inoculation using the injection head on the inoculation head, resulting in a small amount of bacterial solution used and minimal leaf damage. The length of the telescopic tube can be adjusted according to the height of the cotton plant, facilitating operation and improving work efficiency. The amount of bacterial solution used can be adjusted via a button, resulting in a high inoculation success rate and good screening effect for transgenic cotton plants.

Description

Technical Field

[0001] This utility model belongs to the field of cotton breeding technology, specifically a device for rapid inoculation of cotton plants with kanamycin. Background Technology

[0002] In conventional cotton breeding, kanamycin is used to screen transgenic cotton progeny carrying the npt-II marker gene. During the cotton seedling stage, a certain concentration (e.g., 5000 mg / L) of kanamycin solution is applied to the young leaves or the first 1-2 leaves from the bottom using a brush or similar tool. The presence of transgenic plants is determined by whether the applied area turns yellow or withers. To prevent uneven application or excessive application that could lead to large-area leaf damage and false positives, the application needs to be repeated 2-3 times during the seedling stage. Therefore, manual inoculation with kanamycin using tools such as brushes is costly, time-consuming, labor-intensive, causes significant plant damage, is inefficient, and yields poor screening results. Utility Model Content

[0003] The purpose of this invention is to provide a device for rapid inoculation of cotton plants with kanamycin, so as to solve the problems mentioned in the background art.

[0004] The technical solution adopted in this utility model is as follows:

[0005] A device for rapid kanamycin inoculation of cotton plants includes a handle, a telescopic tube, and an inoculation head arranged sequentially from left to right. The left end of the handle is a bacterial solution storage chamber, and the right end of the handle is an equipment chamber. The equipment chamber is equipped with a telescopic tube reel. One end of the telescopic tube reel passes through the telescopic tube and is connected to the inoculation head. The other end of the telescopic tube reel is connected to the bacterial solution storage chamber through a valve and a diaphragm pump.

[0006] Preferably, the handle is provided with a diaphragm pump adjustment button and a valve switch on the outside, and the diaphragm pump adjustment button and the valve switch control the diaphragm pump and the valve respectively.

[0007] Preferably, the left end of the bacterial solution storage chamber is screwed with a rear cover for adding bacterial solution and cleaning the bacterial solution storage chamber.

[0008] Preferably, the inoculation head has a hollow rectangular structure with several injection heads on its ground, and the injection heads are connected to the inner cavity of the inoculation head.

[0009] Preferably, the equipment compartment is also equipped with a battery for powering the diaphragm pump and valves.

[0010] Preferably, the valve is a solenoid valve, and the diaphragm pump is model MY320_5V.

[0011] In summary, due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0012] In this invention, the injection head on the inoculation head is used for precise inoculation, which requires less bacterial solution and minimizes leaf damage. The length of the telescopic rod can also be adjusted according to the height of the cotton plant, making it convenient to operate and improving work efficiency. The amount of bacterial solution used can be adjusted by a button, resulting in a high inoculation success rate and good screening effect for transgenic cotton plants. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0014] Figure 2 This is a cross-sectional view of the present invention;

[0015] In the diagram: 1. Handle; 2. Telescopic tube; 3. Inoculation head; 31. Injection head; 4. Bacterial liquid storage chamber; 41. Rear cover; 5. Equipment compartment; 6. Telescopic tube reel; 7. Diaphragm pump; 71. Diaphragm pump adjustment button; 8. Battery; 9. Valve; 91. Valve switch. Detailed Implementation

[0016] The specific embodiments of this utility model are described in detail below.

[0017] The "range" disclosed in this utility model is defined by a lower limit and an upper limit. A given range is defined by selecting a lower limit and an upper limit, which define the boundaries of a particular range. Ranges defined in this way can include or exclude endpoints and can be arbitrarily combined; that is, any lower limit can be combined with any upper limit to form a range. For example, if a range of 10–50 is listed for a specific parameter, it is also expected that ranges of 10–40 and 20–50 are also included. Furthermore, if the minimum range values ​​are 1 and 2, and the maximum range values ​​are 3, 4, and 5, then the following ranges are all expected: 1–3, 1–4, 1–5, 2–3, 2–4, and 2–5. In this application, unless otherwise stated, the range of values ​​"a–b" represents a shortened representation of any combination of real numbers between a and b, where a and b are real numbers. For example, the range of values ​​"0–5" means that all real numbers between "0–5" have been listed herein; "0–5" is merely a shortened representation of these combinations of values.

[0018] Unless otherwise specified, all embodiments and optional embodiments of this application can be combined to form new technical solutions.

[0019] Unless otherwise specified, all technical features and optional technical features of this application may be combined to form new technical solutions.

[0020] Unless otherwise specified, all steps in this application may be performed sequentially or randomly, preferably sequentially. For example, the method includes steps (a) and (b), indicating that the method may include steps (a) and (b) performed sequentially, or it may include steps (b) and (a) performed sequentially. For example, the mention that the method may also include step (c) indicates that step (c) may be added to the method in any order. For example, the method may include steps (a), (b), and (c), or it may include steps (a), (c), and (b), or it may include steps (c), (a), and (b), etc.

[0021] Unless otherwise specified, the terms "comprising" and "including" as used in this application can be open-ended or closed-ended. For example, "comprising" and "including" can mean that other components not listed may also be included, or that only the listed components may be included.

[0022] Unless otherwise specified, the reaction will proceed under normal temperature and pressure conditions.

[0023] Unless otherwise specified, all parts or percentages are by weight or by weight percentage.

[0024] In this invention, all the substances used are known substances that can be purchased or synthesized by known methods.

[0025] In this invention, all the devices or equipment used are conventional devices or equipment known in the art and are readily available.

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this utility model and are not intended to limit this utility model.

[0027] Example:

[0028] A device for rapid inoculation of cotton plants with kanamycin, such as... Figure 1-2 As shown, the device includes a handle 1, a telescopic tube 2, and an inoculation head 3 arranged sequentially from left to right. The left end of the handle 1 is a bacterial culture storage chamber 4, and the right end of the handle 1 is an equipment chamber 5. The equipment chamber 5 is equipped with a telescopic tube reel 6. One end of the telescopic tube reel 6 passes through the telescopic tube 2 and is connected to the inoculation head 3. The other end of the telescopic tube reel 6 is connected to the bacterial culture storage chamber 4 through a valve 9 and a diaphragm pump 7.

[0029] In one possible implementation, a diaphragm pump adjustment button 71 and a valve switch 91 are provided on the outside of the handle 1. The diaphragm pump adjustment button 71 and the valve switch 91 control the diaphragm pump 7 and the valve 9, respectively.

[0030] In one possible implementation, a rear cover 41 is screwed onto the left end of the bacterial solution storage chamber 4 for adding bacterial solution and cleaning the bacterial solution storage chamber 4.

[0031] In one possible implementation, the inoculation head 3 has a hollow rectangular structure with several injection heads 31 on its ground, and the injection heads 31 are connected to the inner cavity of the inoculation head 3.

[0032] In one possible implementation, the equipment compartment 5 is also equipped with a battery 8 for powering the diaphragm pump 7 and the valve 9.

[0033] In one possible implementation, valve 9 is a solenoid valve, and diaphragm pump 7 is model MY320_5V.

[0034] In one possible implementation, the telescopic tube 2 is formed by sequentially connecting several thin tubes, and the telescopic tube reel 6 is a commercially available electric / spring tube reel. The electric tube reel is connected to a battery, and the liquid inlet tube is wound on the telescopic tube reel 6. One end of the tube passes through the thin tube and is connected to the inoculation head 3. As the telescopic tube 2 stretches / contracts, the liquid inlet tube also stretches / contracts accordingly.

[0035] In one possible implementation, the inoculation head 3 has 7 raised injection heads 31, which makes the inoculation more precise and requires less bacterial solution than traditional spraying or smearing methods. After the leaves turn yellow in some areas, 7 regularly arranged spots will form, which are easy to identify and can effectively distinguish between diseases, pesticide damage and other leaf damage symptoms.

[0036] In one possible implementation, the length of the telescopic rod can be manually adjusted when using the device. The telescopic rod is divided into 3 sections, with a length of 90m, and can be used for bacterial inoculation of cotton plants with different plant types and growth stages at the first or second leaf from the bottom in most scenarios.

[0037] In one possible implementation, the amount of bacterial solution is adjusted by adjusting the diaphragm pump adjustment button 71. The bacterial solution is delivered using a MY320-5V miniature diaphragm pump, which can achieve precise control of negative pressure, time and bacterial solution volume, uniform atomization of bacterial solution, strong infectivity and not easy to fall off.

[0038] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for rapid inoculation of cotton plants with kanamycin, characterized in that: It includes a handle (1), a telescopic tube (2) and an inoculation head (3) arranged from left to right. The left end of the handle (1) is a bacterial culture storage chamber (4), and the right end of the handle (1) is an equipment chamber (5). The equipment chamber (5) is equipped with a telescopic tube reel (6). One end of the telescopic tube reel (6) passes through the telescopic tube (2) and is connected to the inoculation head (3). The other end of the telescopic tube reel (6) is connected to the bacterial culture storage chamber (4) through a valve (9) and a diaphragm pump (7).

2. The device for rapid inoculation of cotton plants with kanamycin as described in claim 1, characterized in that: The handle (1) is provided with a diaphragm pump adjustment button (71) and a valve switch (91) on the outside. The diaphragm pump adjustment button (71) and the valve switch (91) control the diaphragm pump (7) and the valve (9) respectively.

3. The device for rapid kanamycin inoculation of cotton plants as described in claim 1, characterized in that: The bacterial liquid storage chamber (4) has a rear cover (41) screwed onto its left end for adding bacterial liquid and cleaning the bacterial liquid storage chamber (4).

4. The device for rapid kanamycin inoculation of cotton plants as described in claim 1, characterized in that: The inoculation head (3) adopts a hollow rectangular structure, and several injection heads (31) are provided on its ground. The injection heads (31) are connected to the inner cavity of the inoculation head (3).

5. The device for rapid kanamycin inoculation of cotton plants as described in claim 2, characterized in that: The equipment compartment (5) is also equipped with a battery (8) for powering the diaphragm pump (7) and valves (9).

6. The device for rapid inoculation of cotton plants with kanamycin as described in claim 1, characterized in that: The valve (9) is a solenoid valve, and the diaphragm pump (7) is model MY320_5V.

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