Tissue marking device and tissue preservation equipment containing the same

Abstract

This invention provides a tissue marking device and a tissue preservation apparatus including the same. The tissue marking device includes a marker, a connector, and a container; the connector connects the marker and the container; the container has an elastic mesh structure with a container opening that can switch between an open and closed position. This tissue marking device can securely fix ex vivo tissue and stably connect it to the marker. The container's elastic mesh structure can adapt to ex vivo tissues of different shapes and sizes, avoiding damage caused by mechanical pressure; the container opening can switch between open and closed positions, facilitating quick insertion and removal of ex vivo tissues, simplifying operation. The connector stably connects the marker and the container, preventing the loss or confusion of marking information during processing, ensuring the full traceability of the original biological information of the ex vivo tissue, and allowing the connector to remain unimmersed.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental instruments, and in particular to a tissue labeling device and a tissue preservation device containing the same. Background Technology

[0002] In existing technologies, ex vivo tissues from laboratory animals require a lengthy process of sample preparation and preservation. In drug trials, key organs such as the heart, liver, lungs, and kidneys, as well as tissues suspected of being diseased, typically require a sample preparation process of six months to two years, involving changing the cut surface and preparing new slides. After the drug is marketed, the ex vivo tissues must be preserved for five to ten years to meet compliance requirements.

[0003] The excised tissues vary in size, but all require formalin fixation, dehydration and clearing, and paraffin embedding, followed by long-term immersion preservation. After processing, the tissue morphology will undergo irreversible changes, making it impossible to trace the original anatomical location and biological characteristics through conventional methods (such as visual observation and tissue block numbering). Therefore, effective marking is necessary immediately after excision.

[0004] In current routine procedures, operators typically attempt to record the original anatomical location and biological characteristics of excised tissues using methods such as affixing labels, directly marking with markers, and binding with cable ties. However, these methods still have significant limitations:

[0005] Firstly, labels are prone to detachment and information distortion. After being soaked in fixatives such as formalin, the moist tissue releases tissue fluid due to changes in osmotic pressure, causing the adhesive layer of paper or plastic label media to dissolve and fail. This process leads to label edge warping, ink diffusion and smudging, resulting in label detachment, confusion, and ultimately, difficulty in tracing the original data.

[0006] Secondly, small-volume ex vivo tissues have poor labeling compatibility. Due to insufficient surface area, smoothness, or lack of effective fixation sites, micro-tissues are difficult to label using traditional writing or pasting methods. Current methods rely on embedding cassettes for preservation, but the inner diameter of the embedding cassettes imposes high requirements on the volume and shape of the tissue. When a large number of micro-tissues need to be collected, multiple embedding cassettes are required, significantly increasing the workload of experimental recording and file management. In addition, an additional embedding cassette number-tissue correspondence record table is required, increasing the complexity of original record management. Physical labels and record files must be saved simultaneously during archiving, resulting in resource waste.

[0007] Third, mechanical fixation methods can damage sample integrity. Traditional plastic / metal cable ties with tags can easily cause local compression damage and deformation due to the mechanical pressure applied to the tissue. Furthermore, the cable ties lack elasticity after fixation and can slip off after tissue dehydration, affecting subsequent staining, slide preparation, and microscopic examination. Utility Model Content

[0008] The technical problem to be solved by this utility model is to overcome the defects of existing technology in which ex vivo tissue labels and ex vivo tissues are difficult to stably bind, and to provide a tissue marking device and a tissue preservation device containing the same.

[0009] The present invention solves the above-mentioned technical problems through the following technical solution:

[0010] A tissue marking device comprising a marker;

[0011] The tissue marking device also includes a connector and a container;

[0012] The connector is used to connect the marker and the container;

[0013] The container is a flexible mesh structure with a holding opening that can switch between an open and closed position.

[0014] In this technical solution, by providing the tissue marking device, isolated tissues can be securely fixed and stably connected to the marking component. The container adopts an elastic mesh structure, which can adapt to isolated tissues of different shapes and sizes, avoiding damage to the isolated tissues caused by mechanical pressure, and facilitating the penetration of the soaking and preservation liquid. The container opening can be switched between open and closed positions, facilitating the rapid insertion and removal of isolated tissues, and simplifying operation. The connector stably connects the marking component and the container, preventing the loss or confusion of marking information during fixation, dehydration, embedding, and other processes, thereby ensuring the full traceability of the original biological information of the isolated tissue (such as anatomical location and morphological characteristics), and allowing the connector to remain unsoaked when the container is soaked for preservation.

[0015] Preferably, the elasticity of the middle portion of the container is stronger than the elasticity of the outer periphery of the container; and / or,

[0016] The void density of the container is 100-150 mesh.

[0017] In this technical solution, by setting the elasticity of the middle part of the container to be stronger than that of the outer periphery, the net bag can better fit the tissue surface, which is especially suitable for irregularly shaped or small-volume ex vivo tissues. It allows the tissues to be naturally fixed in the middle of the container and provides uniform support, avoiding local compression and deformation, and the periphery can be in relatively uniform contact with the liquid used for soaking and preservation.

[0018] By setting the porosity of the container to 100-150 mesh, it is possible to ensure that the liquid fully penetrates during immersion preservation to improve the preservation effect of the ex vivo tissue, while also effectively preventing leakage of the ex vivo tissue to ensure the integrity of the sample.

[0019] Preferably, the container opening is provided with a magnetic latch;

[0020] When the container opening is in the closed position, the magnetic latch is engaged;

[0021] When the container opening is in the open position, the magnetic latch disengages.

[0022] In this technical solution, the above-mentioned settings enable rapid opening and automatic closing of the holding opening, eliminating the need for complex operations such as knotting and cutting, simplifying the operation process, and facilitating the re-storage of ex vivo tissue samples after multiple extractions and re-processing. The magnetic lock automatically adheres and locks in the closed position, ensuring a secure seal of the holding opening, preventing accidental detachment of the ex vivo tissue during processing, and improving the reliability of the tissue marking device.

[0023] Preferably, the surface of the magnetic latch is covered with a protective layer.

[0024] In this technical solution, the above-mentioned settings prevent the latch from coming into direct contact with the detached tissue or the liquid used for soaking and preservation, thus avoiding chemical corrosion of the latch or physical damage to the detached tissue and ensuring reliability in long-term use.

[0025] Preferably, the holding opening is located at the top of the holding component.

[0026] In this technical solution, the above-mentioned settings facilitate the operator to directly place or remove the detached tissue from above, improving operational efficiency and preventing the detached tissue from slipping out of the container due to gravity.

[0027] Preferably, one end of the connector is connected to the lower part of the holding opening.

[0028] In this technical solution, the above-mentioned settings prevent the connector from getting entangled with the detached tissue or interfering with the closure of the container; at the same time, they keep the container balanced when suspended, preventing the detached tissue from tilting or shifting excessively and improving stability.

[0029] Preferably, the connector is a cord; and / or,

[0030] One end of the connector is connected to the inside of the container; and / or,

[0031] The length of the connector is adjustable; and / or,

[0032] The connector is made of a biocompatible metallic material or a biocompatible polymer material;

[0033] The diameter of the connector is 0.3mm to 0.5mm.

[0034] In this technical solution, by setting the connector as a rope, it is easy for the connector to pass through the sealed area and the position of the marker and the container can be adjusted accordingly.

[0035] By setting one end of the connector to be connected to the inside of the container, when the connection between the connector and the container comes loose, the container opening in the closed position can further limit the connector and prevent the container from slipping directly off the connector.

[0036] The length of the connector can be adjusted to accommodate storage containers of different depths and shapes.

[0037] By setting the material of the connector to be a biocompatible metal or a biocompatible polymer, which is corrosion-resistant and safe and non-toxic, the durability and safety of the connector are improved.

[0038] By setting the diameter of the connector to 0.3mm-0.5mm, both strength and space-saving measures are ensured, guaranteeing the reliable and durable use of the marking information throughout the process.

[0039] Preferably, the marker is made of synthetic paper or resin-based material; and / or,

[0040] The marking element is a rectangular sheet with a side length of 20mm-40mm.

[0041] In this technical solution, by setting the marker to be made of synthetic paper or resin, the marker has excellent writing properties and solvent resistance, and the writing remains clear and does not smudge when soaked in liquids such as formalin.

[0042] By setting the markers as rectangular pieces with sides of 20mm-40mm, ample writing area is provided for recording detailed information, while the moderate size makes them easy to hang and identify.

[0043] A tissue preservation device includes the tissue marking device as described above.

[0044] In this technical solution, by providing the tissue preservation device, the above-mentioned marking device is integrated, realizing the integration of in vitro tissue preservation and marking, simplifying the operation process, improving work efficiency, ensuring that the marking information of the in vitro tissue is not lost during the preservation process, enhancing the traceability of experimental data, and facilitating management.

[0045] Preferably, the tissue preservation device further includes a preservation container having a preservation opening, the connector passing through the preservation opening such that the holding member is located inside the preservation container and the marker is located outside the preservation container.

[0046] In this technical solution, the above-mentioned settings facilitate the immersion preservation of ex vivo tissues while preventing the markers from being contaminated by the immersion preservation liquid.

[0047] The positive and progressive effects of this utility model are as follows:

[0048] This tissue marking device enables the secure fixation of ex vivo tissues and their stable connection to the marking components. The container, with its elastic mesh structure, can adapt to ex vivo tissues of different shapes and sizes, avoiding damage caused by mechanical pressure and facilitating the penetration of the immersion preservation liquid. The container opening can be switched between open and closed positions for quick insertion and removal of ex vivo tissues, simplifying operation. The connector stably connects the marking components to the container, preventing the loss or confusion of marking information during fixation, dehydration, embedding, and other processes. This ensures the full traceability of the original biological information of the ex vivo tissue (such as anatomical location and morphological characteristics) and allows the connector to remain unimmersed while the container is immersed for preservation.

[0049] By providing this tissue preservation device, which integrates the aforementioned labeling device, the preservation and labeling of ex vivo tissues are integrated, simplifying the operation process and improving work efficiency; it ensures that the labeling information of ex vivo tissues is not lost during the preservation process, enhances the traceability of experimental data, and facilitates management. Attached Figure Description

[0050] Figure 1 This is a schematic diagram of the structure of a tissue marking device according to an embodiment of the present invention.

[0051] Figure 2 This is a schematic diagram of the tissue marking device in use according to an embodiment of the present invention.

[0052] Figure 3 This is a schematic diagram of the structure of a tissue preservation device according to an embodiment of the present invention.

[0053] Explanation of reference numerals in the attached figures:

[0054] Tissue Marking Device 1

[0055] Marker 11

[0056] Connector 12

[0057] Container 13

[0058] Blooming Opening 131

[0059] Storage Container 2

[0060] Preservation opening 21

[0061] 3 Ex vivo tissues Detailed Implementation

[0062] The present invention will be described more clearly and completely below with reference to the accompanying drawings, using a preferred embodiment.

[0063] like Figure 1 , Figure 2 As shown, this embodiment provides a tissue marking device 1, which includes a marking element 11, a connector 12, and a holding element 13; the connector 12 is used to connect the marking element 11 and the holding element 13; the holding element 13 is an elastic mesh structure, and the holding element 13 has a holding opening 131, which can switch between an open position and a closed position.

[0064] In this embodiment, by providing the tissue marking device 1, the ex vivo tissue 3 can be securely fixed and stably connected to the marking element 11. The container 13 adopts an elastic mesh structure, which can adapt to ex vivo tissues 3 of different shapes and sizes, avoid damage to the ex vivo tissue 3 caused by mechanical pressure, and facilitate the penetration of the liquid for immersion preservation; the container opening 131 can switch between open and closed positions, which facilitates the quick insertion and removal of the ex vivo tissue 3 and is easy to operate. The connector 12 stably connects the marking element 11 and the container 13, preventing the loss or confusion of marking information during fixation, dehydration, embedding and other processes, thereby ensuring the full traceability of the original biological information (such as anatomical location and morphological characteristics) of the ex vivo tissue 3, and allowing the connector 12 to remain unimmersed when the container 13 is immersed for preservation.

[0065] In this embodiment, the elasticity of the middle part of the holding member 13 is stronger than that of the outer periphery, allowing the net to better conform to the tissue surface, which is especially suitable for irregularly shaped or small-volume ex vivo tissues 3 (in Figure 2 The example shown is a hexagon, but the morphology of the ex vivo tissue 3 is complex and diverse and is not limited to this. This allows it to be naturally fixed in the middle of the container 13, providing uniform support, avoiding local compression deformation, and ensuring that the periphery can be in relatively uniform contact with the liquid used for soaking and preservation.

[0066] In this embodiment, the porosity of the container 13 is 100 mesh, which ensures sufficient liquid penetration during immersion preservation to enhance the preservation effect of the ex vivo tissue 3, while effectively preventing leakage of the ex vivo tissue 3 to ensure sample integrity. Of course, in other embodiments, the porosity of the container 13 can also be other values ​​in the range of 100-150 mesh to achieve similar effects.

[0067] In this embodiment, a magnetic latch (not shown in the figure) is provided at the holding opening 131; when the holding opening 131 is in the closed position, the magnetic latch is engaged; when the holding opening 131 is in the open position, the magnetic latch is disengaged. This enables rapid opening and automatic closing of the holding opening 131, eliminating the need for complex operations such as knotting and cutting, simplifying the operation process, and facilitating repeated extraction and re-storage of the ex vivo tissue 3 after sample preparation. The magnetic latch automatically engages and locks in the closed position, ensuring a secure seal of the holding opening 131, preventing accidental detachment of the ex vivo tissue 3 during processing, and improving the reliability of the tissue marking device 1.

[0068] In this embodiment, the surface of the magnetic latch is covered with a protective layer to prevent the latch from directly contacting the detached tissue 3 or the liquid used for soaking and preservation, thereby avoiding chemical corrosion of the latch or physical damage to the detached tissue 3 and ensuring reliability in long-term use.

[0069] In this embodiment, the protective layer is made of medical-grade silicone, which does not react with tissue or fixative and can protect the isolated tissue 3 from physical damage during impact. In other embodiments, the protective layer can also be made of other existing bio-inert or soft materials to achieve similar effects.

[0070] In this embodiment, the holding opening 131 is located at the top of the holding component 13, which makes it convenient for the operator to directly put in or take out the excised tissue 3 from above, improving the efficiency of operation, and also preventing the excised tissue 3 from slipping out of the holding component 13 due to gravity.

[0071] In this embodiment, one end of the connector 12 is connected to the lower part of the holding opening 131 to prevent the connector 12 from getting tangled with or interfering with the closure of the holding component 13; at the same time, it keeps the holding component 13 balanced when suspended, preventing the detached tissue 3 from tilting or shifting excessively and improving stability.

[0072] In this embodiment, the connector 12 is a rope, which allows the connector 12 to pass through the sealed area and to adaptively adjust the positions of the marker 11 and the container 13.

[0073] In this embodiment, one end of the connector 12 is connected to the inside of the container 13. When the connection between the connector 12 and the container 13 falls off, the container opening 131, which is in the closed position, can further limit the connector 12 and prevent the container 13 from slipping directly off the connector 12.

[0074] In this embodiment, the length of the connector 12 is adjustable to adapt to storage containers 2 of different depths and shapes;

[0075] In this embodiment, the material of connector 12 is a biocompatible metal material, which is corrosion-resistant and safe and non-toxic, improving the durability and safety of connector 12; in other embodiments, the material of connector 12 can also be a biocompatible polymer material to achieve a similar effect.

[0076] In this embodiment, the diameter D of the connector 12 is 0.3 mm, which ensures strength while avoiding excessive space occupation, ensuring that the marking information is durable and reliable in the process. In other embodiments, the diameter of the connector 12 can also take other values ​​in the range of 0.3 mm to 0.5 mm.

[0077] In this embodiment, the marker 11 is made of synthetic paper, which gives it excellent writeability and solvent resistance, ensuring that the writing remains clear and does not smudge when immersed in liquids such as formalin. In other embodiments, the marker 11 may also be made of resin-based materials to achieve a similar effect.

[0078] The marker 11 is a rectangular sheet with a length d1 = 40 mm and a width d2 = 20 mm, providing ample writing area for recording detailed information. Its moderate size also facilitates hanging and identification. In other embodiments, the side length of the marker 11 can take other values ​​within the range of 20 mm to 40 mm to achieve a similar effect.

[0079] In this embodiment, one end of the connector 12 passes through the hole in the marker 11 and is knotted for fixation; the other end of the connector 12 is knotted and fixed to the mesh structure of the container 13, ensuring that it does not loosen or dissolve during the entire process of fixation, dehydration, and embedding, thus achieving full traceability of the marking information and the sample. In other embodiments, the two ends of the connector 12 can also be firmly connected to the marker 11 and the container 13 by means of existing technologies such as heat fusion, UV curing, or mechanical connection.

[0080] like Figure 3 As shown, this embodiment also provides a tissue preservation device, which includes the tissue marking device 1 as described above. By integrating the marking device, the preservation and marking of the ex vivo tissue 3 are integrated, simplifying the operation process and improving work efficiency; ensuring that the marking information of the ex vivo tissue 3 is not lost during the preservation process, enhancing the traceability of experimental data, and facilitating management.

[0081] In this embodiment, the tissue preservation device also includes a preservation container 2, which has a preservation opening 21. A connector 12 passes through the preservation opening 21, so that the container 13 is located inside the preservation container 2 and the marker 11 is located outside the preservation container 2, which facilitates the immersion preservation of the ex vivo tissue 3 and avoids the marker 11 from being contaminated by the immersion preservation liquid.

[0082] In this embodiment, all components of the tissue preservation device are made of materials that do not react with conventional fixative components such as formaldehyde, ethanol, and formalin, ensuring that the tissue preservation device remains stable and unreactive during long-term preservation, thus achieving long-lasting preservation.

[0083] While specific embodiments of this utility model have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of this utility model is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of this utility model, but all such changes and modifications fall within the scope of protection of this utility model.

Claims

1. A tissue marking device comprising a marking element, characterized in that: The tissue marking device also includes a connector and a container; The connector is used to connect the marker and the container; The container is a flexible mesh structure with a holding opening that can switch between an open and closed position.

2. The tissue marker device of claim 1, wherein, The elasticity of the middle portion of the container is stronger than the elasticity of the outer periphery of the container; and / or, The void density of the container is 100-150 mesh.

3. The tissue marker device of claim 1, wherein, The container opening is equipped with a magnetic latch; When the container opening is in the closed position, the magnetic latch is engaged; When the container opening is in the open position, the magnetic latch disengages.

4. The tissue marking device as claimed in claim 3, characterized in that, The surface of the magnetic latch is covered with a protective layer.

5. The tissue marking device as claimed in claim 1, characterized in that, The holding opening is located at the top of the holding component.

6. The tissue marker device of claim 5, wherein, One end of the connector is attached to the bottom of the holding opening.

7. The tissue marking device as claimed in claim 1, characterized in that, The connector is a cord; and / or, One end of the connector is connected to the inside of the container; and / or, The length of the connector is adjustable; and / or, The connector is made of a biocompatible metallic material or a biocompatible polymer material; The diameter of the connector is 0.3mm to 0.5mm.

8. The tissue marking device as claimed in claim 1, characterized in that, The marking element is made of synthetic paper or resin material; and / or, The marking element is a rectangular sheet with a side length of 20mm-40mm.

9. A tissue preservation apparatus, characterized by, It includes the tissue marking device as described in any one of claims 1-8.

10. The tissue preservation device as described in claim 9, characterized in that, The tissue preservation device further includes a preservation container having a preservation opening, the connector passing through the preservation opening such that the holding member is located inside the preservation container and the marker is located outside the preservation container.

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