A nucleic acid purification device for biological sample processing

Abstract

This invention belongs to the field of nucleic acid purification technology, specifically relating to a nucleic acid purification device for biological sample processing. It includes a column body, an adsorbent material, a collection tube, and a central space filling mechanism. An outlet is fixedly connected to the lower end of the column body. The adsorbent material is fixedly connected inside the column body. The collection tube is installed on the outer side of the column body. The space inside the collection tube, located on the lower side of the column body, is the main collection chamber. The central space filling mechanism is installed inside the main collection chamber. The inner diameter of the collection tube is larger than the outer diameter of the column body, forming an annular collection chamber between the inner wall of the collection tube and the outer wall of the column body. This invention can increase the contact area between the eluent and the inner wall of the collection tube during water bath heating of the eluent, thereby increasing the heated area of ​​the eluent and improving heating efficiency.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid purification technology, specifically relating to a nucleic acid purification device for biological sample processing. Background Technology

[0002] In molecular biology experiments, nucleic acid purification is a fundamental step in subsequent gene amplification, sequencing, and hybridization experiments. Its core is the separation of nucleic acids from impurities using components such as columns and adsorption materials, with the target nucleic acid ultimately collected using an elution buffer. Most current mainstream nucleic acid purification devices employ the classic "adsorption-washing-elution" process. Adsorption materials such as silica membranes, glass fibers, or ion exchange resins specifically bind to nucleic acids under specific buffer conditions. A washing buffer removes impurities such as proteins and salts, and finally, an elution buffer releases the nucleic acid from the adsorption material and collects it in a collection tube.

[0003] However, existing nucleic acid purification devices still have significant shortcomings in the post-elution processing of the eluent. In some experimental scenarios, the eluted nucleic acids need to be subjected to water bath heating, such as for the complete dissolution of high-concentration nucleic acids, denaturation and denaturation of double-stranded nucleic acids, or removal of trace residual impurities. However, the collection tubes of traditional devices are mostly single-cavity structures, and the eluent naturally accumulates at the bottom or center of the collection tube, resulting in a limited contact area between the eluent and the inner wall of the collection tube, and low heat transfer efficiency. Utility Model Content

[0004] The purpose of this invention is to provide a nucleic acid purification device for biological sample processing, which can increase the contact area between the eluent and the inner wall of the collection tube during the water bath heating process, thereby increasing the heated area of ​​the eluent and improving the heating efficiency.

[0005] The specific technical solution adopted by this utility model is as follows:

[0006] A nucleic acid purification device for biological sample processing includes a column body, an adsorbent material, a collection tube, and a central space filling mechanism;

[0007] The lower end of the column tube is fixedly connected to a discharge port, the adsorbent material is fixedly connected inside the column tube, and the collection tube is installed on the outside of the column tube.

[0008] The space inside the collection tube located on the lower side of the column tube is the main collection cavity, and the central space filling mechanism is installed inside the main collection cavity.

[0009] Furthermore, the inner diameter of the collecting tube is larger than the outer diameter of the column tube, and an annular collecting cavity is formed between the inner wall of the collecting tube and the outer wall of the column tube.

[0010] Furthermore, the central space filling mechanism includes a flexible inflatable expansion column installed at the center of the main collection cavity, and the flexible inflatable expansion column is connected to an inflation assembly.

[0011] Furthermore, the inflation assembly includes an air guide pipe fixedly connected to the lower axis of the collecting tube. One end of the air guide pipe is located outside the collecting tube, and a valve is installed on the pipe body outside the collecting tube. The other end of the air guide pipe is located inside the collecting tube. The flexible inflatable expansion column is fixedly connected to the outside of the pipe body inside the collecting tube. A main air vent is opened at the end of the air guide pipe, and multiple secondary air vents are opened on the periphery of the air guide pipe.

[0012] Furthermore, a connecting ring is fixedly connected to the upper end of the collecting tube, and an external thread is provided on the outside of the column tube body. The connecting ring is threadedly connected to the outside of the external thread of the column tube body.

[0013] Furthermore, a top cover is detachably installed at the upper end of the column tube.

[0014] The technical effects achieved by this utility model are as follows:

[0015] This invention relates to a nucleic acid purification device for biological sample processing. By setting a central space filling mechanism to expand and fill the space in the middle of the main collection chamber, the eluent inside the main collection chamber is squeezed towards the side wall of the collection tube, reducing the local temperature difference phenomenon of "the outer layer is heated while the center is still cold", increasing the contact area between the eluent and the inner wall of the collection tube, increasing the heated area of ​​the eluent, and thus improving the heating efficiency. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0018] Figure 3 This is a schematic diagram of the overall cross-sectional structure of this utility model;

[0019] Figure 4 This is a side view of the cross-sectional structure of this utility model.

[0020] The attached diagram lists the components represented by each number as follows:

[0021] 1. Column body; 2. Top cover; 3. Adsorbent material; 4. Discharge port; 5. Collection pipe; 6. Connecting ring; 7. Air guide pipe; 8. Valve; 9. Main vent; 10. Secondary vent; 11. Flexible inflatable expansion column; 12. Cylindrical liquid. Detailed Implementation

[0022] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0023] like Figures 1-4 As shown, a nucleic acid purification device for biological sample processing includes a column body 1, a top cap 2, an adsorbent material 3, a collection tube 5, and a central space filling mechanism;

[0024] The lower end of the column tube 1 is fixedly connected to the discharge port 4. The column tube 1 and the discharge port 4 are usually made of high-temperature resistant and chemically stable plastics such as polypropylene (PP).

[0025] The adsorption material 3 is fixedly connected inside the column tube 1. The adsorption material 3 can be a silica membrane, glass fiber or ion exchange resin.

[0026] The top cover 2 is detachably installed on the upper end of the column tube 1 to seal the upper opening of the column tube 1. The top cover 2 can be installed by threaded connection, press-fit connection or snap-fit.

[0027] The collecting pipe 5 is installed on the outside of the column tube body 1. It can be directly fixed to the outside of the column tube body 1, or it can be threaded or snapped onto the outside of the column tube body 1. In this technical solution, it is preferable to fix a connecting ring 6 at the upper end of the collecting pipe 5 and provide an external thread on the outside of the column tube body 1. The connecting ring 6 is threaded onto the outside of the external thread of the column tube body 1, so that the collecting pipe 5 can be detachably installed on the outside of the column tube body 1 and the sealing between the collecting pipe 5 and the column tube body 1 is guaranteed.

[0028] At this point, the space inside the collection tube 5 located below the column body 1 is the main collection chamber. The elution solution containing the target nucleic acid after elution can enter the main collection chamber through the discharge port 4.

[0029] The central space filling mechanism is installed inside the main collection chamber. This mechanism expands to fill the space in the middle of the main collection chamber, pushing the eluent inside the main collection chamber towards the side wall of the collection tube 5. Its shape is as follows: Figure 4 As shown in the cylindrical liquid 12, since the eluent is relatively evenly distributed inside the collection tube 5 near the inner wall of the collection tube 5, the local temperature difference phenomenon of "the outer layer has been heated while the center is still low" is reduced, the contact area between the eluent and the inner wall of the collection tube 5 is increased, the heating area of ​​the eluent is increased, and thus the heating efficiency is improved.

[0030] Meanwhile, the inner diameter of the collecting tube 5 is larger than the outer diameter of the column body 1, forming an annular collecting cavity between the inner wall of the collecting tube 5 and the outer wall of the column body 1. This allows the eluent to be squeezed into the annular collecting cavity, further reducing the thickness of the eluent. In addition, the threaded connection of the connecting ring 6 can reduce the leakage of eluent through the connection between the column body 1 and the connecting ring 6.

[0031] like Figures 2-4 As shown, in some embodiments, the central space filling mechanism includes a flexible inflatable expansion column 11 installed at the center of the main collection cavity. The flexible inflatable expansion column 11 can be made of latex or rubber, or other materials with good sealing and deformation properties, such as thermoplastic polyurethane film.

[0032] The flexible inflatable expansion column 11 is connected to an inflation group. When the inflation group is connected to an inflation device, air is injected into the flexible inflatable expansion column 11, causing it to expand until it takes shape. This forces the eluent towards the side wall of the collection tube 5. The shape of the formed flexible inflatable expansion column 11 can be as follows... Figure 4 As shown in the image.

[0033] The inflation assembly includes an air guide pipe 7 fixedly connected to the lower axis of the collection pipe 5. One end of the air guide pipe 7 is located outside the collection pipe 5. A valve 8 is installed on the pipe body of the air guide pipe 7 located outside the collection pipe 5 to control whether the air guide pipe 7 is sealed. The other end of the air guide pipe 7 is located inside the collection pipe 5. A flexible inflatable expansion column 11 is fixedly connected to the outside of the pipe body of the air guide pipe 7 located inside the collection pipe 5. The air guide pipe 7 is a rigid pipe, and its material can also be polypropylene. The flexible inflatable expansion column 11 can be assisted in positioning through the secondary vent 10.

[0034] like Figure 3 As shown, the end of the air guide pipe 7 is provided with a main air vent 9, and multiple secondary air vents 10 are provided on the periphery of the air guide pipe 7. The gas can be transported relatively evenly into the interior of the flexible inflatable expansion column 11 through the main air vent 9 and the secondary air vents 10.

[0035] In other embodiments, the central space filling mechanism includes a pneumatic telescopic column installed at the center of the main collection cavity. The pneumatic telescopic column can also be connected to an inflation group. When the pneumatic telescopic column is inflated, its upper and lower ends extend axially until the pneumatic telescopic column is formed.

[0036] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A nucleic acid purification device for biological sample processing, comprising a column body (1), an adsorbent material (3), and a collection tube (5), characterized in that: It also includes a central space filling mechanism; The lower end of the column tube (1) is fixedly connected to the outlet (4), the adsorbent material (3) is fixedly connected inside the column tube (1), and the collection tube (5) is installed on the outside of the column tube (1). The space inside the collection tube (5) located below the column tube body (1) is the main collection cavity, and the central space filling mechanism is installed inside the main collection cavity.

2. The nucleic acid purification device for biological sample processing according to claim 1, characterized in that: The inner diameter of the collecting tube (5) is larger than the outer diameter of the column tube (1), and an annular collecting cavity is formed between the inner wall of the collecting tube (5) and the outer wall of the column tube (1).

3. A nucleic acid purification device for biological sample processing according to any one of claims 1-2, characterized in that: The central space filling mechanism includes a flexible inflatable expansion column (11) installed at the center of the main collection cavity, and the flexible inflatable expansion column (11) is connected to an inflation group.

4. The nucleic acid purification device for biological sample processing according to claim 3, characterized in that: The flexible inflatable expansion column (11) is made of latex or rubber.

5. The nucleic acid purification device for biological sample processing according to claim 3, characterized in that: The inflation assembly includes an air guide pipe (7) fixedly connected to the lower axis of the collecting pipe (5). One end of the air guide pipe (7) is located outside the collecting pipe (5). A valve (8) is installed on the pipe body of the air guide pipe (7) located outside the collecting pipe (5). The other end of the air guide pipe (7) is located inside the collecting pipe (5). The flexible inflatable expansion column (11) is fixedly connected to the outside of the pipe body of the air guide pipe (7) located inside the collecting pipe (5). A main vent hole (9) is opened at the end of the air guide pipe (7).

6. The nucleic acid purification device for biological sample processing according to claim 5, characterized in that: The air duct (7) has multiple secondary air vents (10) on its periphery.

7. The nucleic acid purification device for biological sample processing according to claim 3, characterized in that: The upper end of the collecting tube (5) is fixedly connected to a connecting ring (6), and an external thread is provided on the outside of the column tube body (1). The connecting ring (6) is threadedly connected to the outside of the external thread of the column tube body (1).

8. The nucleic acid purification device for biological sample processing according to claim 1, characterized in that: The upper end of the column tube (1) is detachably fitted with a top cover (2).

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