A culture well plate assembly for organoid electrophysiological acquisition

Abstract

This invention provides a culture plate assembly for organoid electrophysiological data acquisition, comprising a culture plate cover, a shell, multiple culture wells, an electrode layer, a sealing and insulating layer, and a circuit board. The culture wells are detachably disposed below the culture plate cover and have through holes. An electrode layer is located at the bottom of each well, with a receiving end and an exit end. The receiving end is exposed on the inner surface of the bottom of the culture well for acquiring electrophysiological signals, and the exit end extends through the through hole and is detachably electrically connected to the circuit board. A sealing and insulating layer is disposed around the receiving end to isolate the liquid area. The circuit board is disposed below the culture plate cover and inside the shell, and is detachably electrically connected to the electrode layer for receiving and processing electrophysiological signals. The culture plate cover, circuit board, and shell are detachably and fixedly connected and are all made of transparent material. This invention integrates organoid culture, observation, and batch acquisition of electrophysiological signals, allowing for independent replacement of each layer, facilitating maintenance and flexible configuration.

Description

Technical Field

[0001] This invention belongs to the technical field of organoid culture, electrophysiological acquisition and multi-well culture device, and particularly relates to a culture plate assembly for organoid electrophysiological acquisition. Background Technology

[0002] Organoids, as three-dimensional in vitro models, are frequently used in in vitro laboratory environments and are widely applied in fields such as precision medicine and personalized medicine, disease modeling and mechanism research, regenerative medicine, and organ transplantation. In these research scenarios, organoids typically require long-term culture, observation, and processing in culture plates. However, ordinary culture plates are mainly used to contain organoids and culture medium and usually lack electrophysiological signal acquisition capabilities, making it impossible to monitor the electrophysiological characteristics of organoids, such as neural electrical activity, in real time during the culture process.

[0003] Furthermore, existing culture plate solutions cannot simultaneously acquire electrophysiological signals from multiple organoids in batches. Because observation and acquisition functions are separated, operators must transfer the organoids from the culture plate to a separate electrophysiological acquisition device after completing microscopic observation. This transfer process not only interferes with the physiological state of the organoids, affecting the accuracy of the acquired signals, but also increases the risk of contamination. Simultaneously, existing culture plates, electrodes, and circuit boards are typically integrated and fixed structures, lacking a detachable integrated design between functional modules. This means that the culture wells, electrode layers, and circuit boards cannot be independently disassembled for maintenance or replacement. If any module becomes contaminated or damaged, the entire system often needs to be scrapped, increasing experimental costs and failing to flexibly adapt to electrode configurations with different channel numbers or the experimental needs of different types of organoids.

[0004] Therefore, it is necessary to provide a culture plate assembly that can structurally integrate the culture plate, electrode layer and circuit board to realize the integration of organoid culture, in situ observation and batch electrophysiological signal acquisition, and to have the function of disassembling and independently maintaining and replacing each functional module, thereby reducing the interference caused by organoid sample transfer and the complexity of external electrical connections. Utility Model Content

[0005] The purpose of this invention is to solve the technical problems in the prior art, such as the difficulty of culture plates in accommodating organoid culture, observation and electrophysiological signal acquisition, the risk of signal interference and contamination caused by transferring organoids between different devices, and the lack of detachable integrated design between functional modules, making it impossible to maintain and replace them independently. The invention provides a culture plate assembly for organoid electrophysiological acquisition.

[0006] To achieve the above objectives, the technical solution provided by this utility model is as follows:

[0007] A culture plate assembly for organoid electrophysiological data acquisition includes: a culture plate cover, culture wells, a shell, an electrode layer, an insulating layer, and a circuit board; multiple culture wells are detachably disposed below the culture plate cover for containing culture medium and organoids; each culture well has a threading hole that penetrates the outer wall of the culture well; each culture well has an electrode layer at its bottom, the electrode layer having a acquisition end and an exit end, the acquisition end being exposed on the inner surface of the bottom of the culture well for contacting the organoid to acquire electrophysiological signals; one end of the exit end is connected to the acquisition end, and the other end extends out of the culture well through the threading hole. The electrode layer is used to transmit organoid electrophysiological signals to the circuit board. A sealing and insulating layer is set on the inner surface of the bottom of the culture well and surrounds the periphery of the acquisition end to limit the liquid area in the culture well to the area where the acquisition end is located, so as to prevent the culture medium from seeping into the circuit area. The circuit board is set below the culture well cover and inside the outer shell to receive and process the organoid electrophysiological signals collected by the electrode layer from multiple culture wells. The electrode layer and the circuit board are detachably electrically connected. The culture well cover, the circuit board and the outer shell are detachably fixedly connected. The culture well cover, the culture well and the outer shell are all made of transparent material.

[0008] Furthermore, the culture well plate cover is provided with multiple downward protruding connection holes. The connection holes are hollow cylindrical structures. The inner surface of the connection hole is provided with a thread at the end away from the culture well plate cover, which is used to engage with the thread on the outer surface of the top of the culture well, so as to realize the detachable connection between the culture well and the culture well plate cover.

[0009] Furthermore, it also includes a protective cover plate, which is disposed above the culture well plate cover and is detachably connected to the culture well plate cover. It is used to cover the upper surface of the culture well plate cover to seal the top openings of each connection hole and prevent external contaminants from entering the culture well through the connection holes. The protective cover plate is made of transparent material.

[0010] Furthermore, the number of downward protruding connecting holes provided on the culture well cover plate ranges from 1 to 24.

[0011] Furthermore, the lead-out end is an electrode cable, and the acquisition end is a plurality of electrode contacts arrayed at the bottom of the culture well; the electrode cable passes through a wire hole, and a sealing structure for sealing the gap between the wire hole and the electrode cable is provided; the end of the electrode cable is detachably connected to the circuit board via a board-to-board connector.

[0012] Furthermore, the number of electrode channels in the electrode layer is 2 to 1024.

[0013] Furthermore, the board-to-board connector includes a male connector head located at the end of the electrode cable and a female connector head located on the circuit board. The male connector head and the female connector head are inserted into each other to achieve a detachable electrical connection.

[0014] Furthermore, the circuit board includes multiple circuit modules that are configured one-to-one with the electrode layers in the bottom of multiple culture wells. Each circuit module is electrically connected to the corresponding electrode layer and is used to receive and process the electrophysiological signals of the organoids in the corresponding culture wells.

[0015] Furthermore, the circuit board is a single-board main carrier circuit board, with multiple circuit modules integrated on the single-board main carrier circuit board.

[0016] Furthermore, the protective cover plate has mounting holes at its four corners, the culture well plate cover has through holes at its four corners, and the circuit board has through holes at its four corners. The outer casing contains four hollow positioning posts. The center distance between the mounting holes on the protective cover plate, the through holes on the culture well plate cover, the through holes on the circuit board, and the four hollow positioning posts is the same. The outer diameter of the hollow positioning posts is smaller than the inner diameter of the through holes on the circuit board. The four corner through holes of the circuit board are fitted onto the outside of the hollow positioning posts, positioning the circuit board inside the outer casing. Fasteners pass through the mounting holes on the protective cover plate and the through holes on the culture well plate cover in sequence, then extend into the hollow positioning posts, fixing the protective cover plate, culture well plate cover, circuit board, and outer casing together.

[0017] The advantages of this utility model are:

[0018] 1. This utility model integrates the electrode layer at the bottom of the culture well and surrounds the acquisition end of the electrode layer with a sealing insulating layer, thus confining the liquid area in the culture well to the area where the acquisition end is located. This allows the acquisition end to directly contact the organoid during organoid culture to collect electrophysiological signals, fundamentally eliminating the problem of interference with the physiological state of the organoid caused by transferring the organoid to an independent acquisition device, reducing the risk of external contamination introduced by sample transfer, and realizing the integration of organoid culture, in-situ observation and electrophysiological signal acquisition.

[0019] 2. This utility model sets up multiple circuit modules that correspond one-to-one with the electrode layers in the bottom of multiple culture wells, and uses connectors to detachably connect the electrode layers in the bottom of each culture well to the corresponding circuit modules. This enables the same culture plate assembly to simultaneously collect parallel electrophysiological signals from organoids in multiple culture wells, overcoming the defect of existing culture plates that cannot collect electrical signals from multiple organoids in batches, and significantly improving experimental throughput.

[0020] 3. This utility model uses a threaded connection structure to detachably connect the culture well to the culture well plate cover, uses a connector to detachably electrically connect the electrode layer to the circuit board, and detachably fixes the culture well plate cover, circuit board, and outer shell. This allows the culture well, electrode layer, and circuit board to be independently disassembled and replaced, which facilitates flexible adjustment of the number of electrode channels or replacement of different types of culture wells according to experimental needs. It also avoids the overall scrapping due to contamination or damage of a certain module, thus reducing experimental costs.

[0021] 4. This utility model uses a hollow positioning post inside the outer shell as an alignment and positioning structure. The circuit board passes through the four corner through holes and is fitted onto the hollow positioning post to achieve precise positioning. This ensures that the center distance of the through holes on the culture well plate cover, the through holes on the circuit board, and the hollow positioning post are consistent. This ensures that the female connectors on the circuit board and the male connectors on the electrode lines of the corresponding electrode layers at the bottom of the culture wells are precisely aligned in the vertical direction, guaranteeing the reliability of board-to-board connector insertion. It also provides a basis for the detachable and fixed connection between the culture well plate cover and the outer shell, ensuring precise alignment between the components of each layer, and ensuring accurate docking between each electrode and the corresponding circuit module. This improves the assembly accuracy and consistency of repeated assembly of the multi-well culture well plate assembly.

[0022] 5. The protective cover, culture well plate cover, outer shell, and culture well settings of this utility model are all made of transparent materials, which facilitates real-time observation of organoids and their culture environment from multiple directions, and realizes the simultaneous conduction of electrophysiological acquisition and optical observation. Attached Figure Description

[0023] Figure 1 : A three-dimensional view of the overall appearance of the culture well plate assembly after assembly.

[0024] Figure 2 : A schematic diagram of the layered structure of the culture well plate assembly of this utility model.

[0025] Figure 3 : A cross-sectional structural diagram of the culture well plate assembly of this utility model.

[0026] Figure 4 : Top view of the bottom of a single culture well of this utility model.

[0027] Figure 5 : Front view of a single culture well of this utility model.

[0028] In the figure: 1-Cultivation well plate cover; 2-Outer shell; 3-Fastener; 4-Cultivation well; 5-Sealing insulation layer; 6-Electrode layer; 601-Electrode contact; 602-Electrode cable; 603-Connector male head; 7-Circuit board; 701-Connector female head; 8-Hollow positioning column; 9-Organoid; 10-Cultivation medium. Detailed Implementation

[0029] The embodiments of the present invention are described in detail below. These embodiments are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0030] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0031] This application provides a culture plate assembly for organoid electrophysiological data acquisition, comprising: a culture plate cover 1, a protective cover plate, a housing 2, fasteners 3, culture wells 4, a sealing insulating layer 5, an electrode layer 6, a circuit board 7, and hollow positioning posts 8. The protective cover plate is positioned above the culture plate cover 1. Multiple culture wells 4 are detachably connected to the bottom of the culture plate cover 1 via threads. Each culture well 4 is a barrel-shaped structure with a closed bottom, its internal cavity used to contain culture medium 10 and organoids 9. Each culture well 4 has a through-hole on its side wall, and an electrode layer 6 is provided on the inner bottom surface of each culture well. The electrode layer 6 includes electrode contacts 601 and electrode cables 602. One end of the electrode cable 602 is connected to the electrode contact 601, and the other end extends from the through-hole to the outside of the culture well, extending downwards along the outer wall of the culture well 4, and its end connects to a male connector 603. The electrode contacts 601 are surrounded by a sealing insulating layer 5. The circuit board 7 is located below the culture plate cover 1 and inside the housing 2. The circuit board includes multiple circuit modules, each corresponding to an electrode layer at the bottom of a culture well, for receiving and processing electrophysiological signals from the corresponding organoids within the culture well. Electrode layers 6 are detachably electrically connected via connector males 603 at the ends of their electrode cables 602 to connector females 701 on the circuit board 7. Hollow positioning posts 8 inside the outer shell 2 pass sequentially through through-holes in the circuit board 7 and the culture well cover 1. Fasteners 3 are inserted through mounting holes in the protective cover, then through through-holes in the culture well cover 1, and finally into the hollow positioning posts 8 for tightening, thus securing the protective cover, culture well cover 1, circuit board 7, and outer shell 2 together to form a complete, detachable integrated structure.

[0032] like Figure 1 and Figure 2As shown, the culture well plate cover 1 has a rectangular plate structure. Its lower surface has multiple downward-protruding connection holes, i.e., hollow cylinders, with the number of connection holes ranging from 1 to 24, allowing for selection of different numbers of holes according to experimental needs. The inner surface of the bottom end of each hollow cylinder is threaded, engaging with the threads on the outer surface of the top end of the culture well 4, thus achieving a detachable connection between the culture well 4 and the culture well plate cover 1 through threaded connection. Threaded connection is only one implementation method of detachable connection; other detachable connection methods can also be used between the culture well 4 and the culture well plate cover 1.

[0033] The culture well cover 1 has a through hole at each of its four diagonal corners for installing fasteners 3, i.e., screws. The inner diameter of the four through holes is 0.2 mm larger than the diameter of a standard screw to ensure that the screw can pass through smoothly and connect and secure the culture well cover to the outer shell of the culture well plate. The distance between the through holes and the four edges is 5-6 mm to ensure the structural strength of the edges of the culture well cover. The culture well cover 1 is made of transparent material to facilitate real-time observation of the morphological changes and growth status of the organoids 9 inside the culture wells 4 from above during organoid culture and electrophysiological signal acquisition.

[0034] like Figure 2 As shown, the protective cover is a rectangular flat plate with the same dimensions as the culture well plate cover 1. Mounting holes are located at the four corners, with the inner diameter of the mounting holes matching the inner diameter of the through holes in the culture well plate cover 1. The protective cover is positioned above the culture well plate cover 1 and is fixedly connected to it using fasteners 3. After installation, a 1-3 mm air gap is formed between the lower surface of the protective cover and the upper surface of the culture well plate cover 1. This gap helps buffer external temperature changes and prevents condensation dripping, while not obstructing observation from above. The protective cover is also made of transparent material to ensure an unobstructed field of view. The protective cover effectively prevents dust, aerosols, and other contaminants that may be present in the laboratory environment from entering the culture wells.

[0035] like Figure 3 and Figure 5 As shown, the culture well 4 is a barrel-shaped structure with a closed bottom, and its internal cavity is used to directly place the culture medium 10 and the organoid 9. The outer surface of the top of the culture well 4 is provided with threads that mate with the threaded bottom of the hollow cylinder that protrudes downwards from the culture well cover 1. A wire hole (not shown in the figure due to view obstruction) is opened on the side wall of the culture well 4 near the bottom. The diameter of the wire hole is larger than the width of the electrode cable 602 so that the electrode cable can pass through smoothly. After the electrode cable passes through, the gap of the wire hole can be filled and sealed with sealant or silicone rubber. This side wall opening method avoids the influence of the bottom opening on the wall growth of the organoid and reduces the risk of culture medium leakage. An electrode layer 6 is provided at the bottom of the culture well 4 so that the electrode contacts 601 located at the bottom of the culture well 4 can contact the organoid 9 to collect electrical signals.

[0036] The culture wells 4 are also made of transparent material, facilitating real-time observation of the organoids 9 and culture medium 10 within the wells from the side and bottom. In some embodiments, the number of culture wells 4 can be set from 1 to 24, depending on the number of connection holes in the culture well cover plate 1. The effective culture area of ​​a single culture well 4 is 1.5 to 9.6 cm², with a smaller culture area per well resulting from a higher number of culture wells. In a preferred embodiment, the number of culture wells 4 is 6, with an effective culture area of ​​9.5 to 9.6 cm² per well. This configuration ensures high-throughput acquisition while also accommodating the growth space of the organoids within each well.

[0037] like Figure 4 and Figure 5 As shown, electrode layer 6 is disposed inside the bottom of culture well 4, including multiple electrode contacts 601 and electrode cables 602. In this embodiment, the electrode cables are FPC (Flexible Printed Circuit) cables. The electrode contacts 601 are arrayed at the bottom of the culture well, exposed on the inner surface of the bottom of the culture well 4, and contact the organoid 9 inside the culture well to collect its electrophysiological signals. The number of electrode contacts 601 determines the number of electrode channels, which ranges from 2 to 1024. Different electrode configurations with different numbers of channels can be selected according to experimental requirements such as organoid type and signal acquisition accuracy requirements.

[0038] One end of the FPC flexible flat cable is connected to the electrode contact 601, and the other end passes through the wire hole on the side wall of the culture well 4, then bends downward along the outer wall of the culture well 4 until it connects to the male connector 603. The cable's routing path on the outer wall of the culture well can be secured with tape or clips to maintain neatness. The male connector 603 at the end of the FPC flexible flat cable is used to connect with the female connector 701 on the circuit board 7, enabling the downward transmission of electrical signals to the circuit board. This detachable connection method allows the electrode layer 6 to be independently plugged and unplugged from the circuit board 7, facilitating the replacement of electrode layers with different channel numbers according to different experimental needs.

[0039] A sealing and insulating layer 5 is disposed on the inner surface of the bottom of the culture well, surrounding the electrode contact 601 and covering the bottom surface of the well except for the area where the electrode contact 601 is located. This isolates the liquid contact area inside the culture well from the non-collection area of ​​the electrode layer 6, preventing culture medium leakage to the electrode cable and connector area, thereby reducing the risk of electrical short circuits and ensuring the stability of electrophysiological signal acquisition. Since the electrode cable exits through the wire hole on the side wall of the culture well, rather than passing through the sealing layer from the bottom, the sealing and insulating layer 5 can more completely cover the bottom of the well, further improving the sealing reliability.

[0040] like Figure 3As shown, the circuit board 7 is located below the culture well cover 1 and is surrounded by the outer shell 2. The circuit board 7 has through holes at its four opposite corners to allow hollow positioning posts 8 for alignment and positioning to pass through. The inner diameter of the through holes on the circuit board is 1 mm larger than the inner diameter of the through holes in the culture well cover 1, but the center distance between the through holes on the circuit board is the same as the center distance between the four corner through holes in the culture well cover 1. When the circuit board is loaded into the outer shell, its four edges are 1-2 mm away from the side wall of the outer shell.

[0041] The circuit board 7 has multiple circuit modules corresponding to the electrode layers at the bottom of the multiple culture wells 4, and each circuit module has a female connector 701. The male connector 603 at the end of the FPC flexible flat cable is inserted into the corresponding female connector 701 on the circuit board 7 to achieve a detachable electrical connection between the electrode layer 6 and the circuit board 7, transmitting the electrophysiological signals collected by the electrode layer 6 to the corresponding circuit module on the circuit board 7. The circuit modules in this embodiment can be implemented using existing signal acquisition circuits, and their specific circuit structure and connection principle are not improvements of this utility model, so they will not be described in detail here.

[0042] Circuit board 7 is a single-board main carrier circuit board. Each circuit module is used to integrate the acquired electrophysiological signals onto the single-board main carrier circuit board and then transmit them to the bus, realizing batch parallel signal acquisition of organoids in multiple culture wells. The single-board integration method reduces the number of inter-board connections, reduces the complexity of the circuit system, and is conducive to improving the stability of signal transmission. At the same time, circuit board 7 can be disassembled and replaced as a module.

[0043] like Figure 3 As shown, four hollow positioning posts 8 are provided inside the outer casing 2. These hollow positioning posts serve an alignment and positioning function. The inner diameter of the four hollow positioning posts is the same as the inner diameter of the through holes in the culture well plate cover, and the outer diameter of the hollow positioning posts is 0.8 mm larger than the inner diameter. Because the outer diameter of the hollow positioning posts is smaller than the inner diameter of the four through holes in the circuit board (the inner diameter of the through holes in the circuit board is 1 mm larger than the inner diameter of the through holes in the culture well plate cover 1), the circuit board can pass through the four hollow positioning posts. At the same time, because the center distance of the through holes in the circuit board is the same as the center distance of the four through holes in the culture well plate cover, and the distance between their edges is also the same, the culture well plate cover can perfectly fit the culture well plate outer casing. This ensures the alignment accuracy during the assembly of multi-layer components and provides a basis for the detachable and fixed connection between the culture well plate cover and the outer casing.

[0044] Specifically, the center distances of the mounting holes on the protective cover, the through holes on the culture well cover 1, the through holes on the circuit board 7, and the four hollow positioning posts 8 are the same, enabling precise alignment between the protective cover, the culture well cover 1, the circuit board 7, and the outer shell 2. The inner diameter of the hollow positioning posts 8 is the same as the inner diameter of the through holes in the culture well cover 1, allowing the fasteners 3 to pass through; the outer diameter of the hollow positioning posts 8 is smaller than the inner diameter of the through holes in the circuit board 7, allowing the hollow positioning posts 8 to pass through the through holes in the circuit board 7. The circuit board 7 is fitted around the four hollow positioning posts 8 and placed inside the outer shell 2. The specific form of the hollow positioning posts 8 is not limited, as long as they can cooperate with the four corner through holes of the circuit board 7 to achieve positioning, connect the culture well cover 1, and provide a fastening channel for the fasteners 3.

[0045] The operating procedures for using this culture plate assembly are as follows:

[0046] 1. Assembly and splicing: The culture well 4 is screwed and rotated onto the culture well plate cover 1; the electrode layer 6 is placed at the bottom of the culture well 4; the electrode cable 602 is passed through the wire hole in the side wall and extends downward along the outer wall; a sealing insulation layer 5 is set around the electrode contacts; the male connector 603 at the end of the electrode cable 602 of the electrode layer 6 is inserted and spliced ​​with the female connector 701 on the circuit board 7; the through hole of the circuit board 7 is aligned with the four hollow positioning posts 8 inside the outer shell 2 and fitted downward, so that the hollow positioning posts 8 The circuit board 7 passes through the through-hole and enters the interior of the outer casing 2. Then, the culture well plate cover 1 is placed on top of the outer casing 2, aligning the four through-holes of the culture well plate cover 1 with the positions of the hollow positioning posts 8. Next, the protective cover plate is placed on top of the culture well plate cover 1, aligning the mounting holes of the protective cover plate with the through-holes of the culture well plate cover. Finally, screws are passed sequentially through the mounting holes of the protective cover plate, the through-holes of the culture well plate cover 1, and into the hollow interior of the hollow positioning posts 8, and tightened to achieve a fixed connection between the protective cover plate, the culture well plate cover 1, and the outer casing 2. This completes the electrical connection between the internal culture wells 4, the electrode layer 6, and the circuit board 7, as well as the mechanical connection between the external protective cover plate, the culture well plate cover, and the outer casing, assembling a complete culture well plate assembly.

[0047] 2. Signal Acquisition: After sterilizing culture well 4, culture medium 10 is first placed in it, and then organoid 9 is placed in culture well 4. Organoid 9 comes into contact with the electrode contact 601 of electrode layer 6. After the electrode contact 601 acquires the electrophysiological signal of organoid 9, it is transmitted to the male connector 603 through electrode cable 602, and then to the corresponding circuit module of circuit board 7 through board-to-board connector. Each circuit module transmits the signal to the bus, and then to the back-end equipment for analysis and processing.

[0048] 3. Disassembly and Replacement: If signals from other organoids are required, unscrew the screws, remove the protective cover and culture well cover 1 in sequence, rotate the culture well 4 to separate it from the culture well cover 1, remove the organoid 9 and culture medium 10 from the culture well 4, and then replace them with new organoid 9 and culture medium 10. If electrodes with different channel numbers are required for the experiment, pull the male connector 603 out of the female connector 701, remove the electrode layer from the culture well, replace it with an electrode and cable with a different channel number, and then put it back into the culture well. Connect the electrode cable to the circuit board through the wiring hole. Similarly, the circuit board 7 can also be removed for replacement or upgrade. It should be noted that the number of channels of the electrode should be compatible with the number of channels of the corresponding circuit module on the circuit board.

[0049] 4. Observation and testing: Since the protective cover, culture well plate cover 1, culture well 4 and outer shell 2 are all made of transparent material, technicians can directly observe from the outside whether the connection between each structure is complete. At the same time, they can observe the morphological changes of organoids 9 and the state of culture medium 10 in culture well 4 in real time, which is convenient for technicians to monitor and record throughout the experiment.

[0050] The above description is only a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this utility model, and these modifications or substitutions should all be covered within the protection scope of this utility model.

Claims

1. A culture plate assembly for organoid electrophysiological data acquisition, characterized in that, include: Culture plate cover, culture wells, outer shell, electrode layer, sealing insulation layer, and circuit board; Multiple culture wells are detachably disposed below the culture well plate cover for containing culture medium and organoids; Each culture well is provided with a threading hole that penetrates the outer wall of the culture well. Each culture well has an electrode layer at its bottom. The electrode layer has a collection end and an exit end. The collection end is exposed on the inner surface of the bottom of the culture well and is used to contact the organoid to collect electrophysiological signals. One end of the exit end is connected to the collection end, and the other end extends from the wire hole to the outside of the culture well and is used to transmit the organoid electrophysiological signals to the circuit board. The sealing and insulating layer is disposed on the inner surface of the bottom of the culture well and surrounds the periphery of the acquisition end, so as to limit the liquid area in the culture well to the area where the acquisition end is located, so as to prevent the culture medium from seeping into the circuit area. The circuit board is disposed below the culture well plate cover and inside the outer shell, and is used to receive and process the organoid electrophysiological signals collected by the electrode layer in multiple culture wells; The electrode layer is detachably electrically connected to the circuit board; The culture well plate cover, the circuit board, and the outer shell are detachably and fixedly connected. The culture well plate cover, the culture wells, and the outer shell are all made of transparent material.

2. The culture plate assembly for organoid electrophysiological data acquisition according to claim 1, characterized in that, The culture well plate cover is provided with a plurality of downward protruding connection holes. The connection holes are hollow cylindrical structures. The inner surface of the connection hole is provided with a thread at the end away from the culture well plate cover, which is used to engage with the thread on the outer surface of the top of the culture well, so as to realize the detachable connection between the culture well and the culture well plate cover.

3. A culture plate assembly for organoid electrophysiological data acquisition according to claim 2, characterized in that, It also includes a protective cover plate, which is disposed above the culture well plate cover and is detachably connected to the culture well plate cover. It is used to cover the upper surface of the culture well plate cover to close the top opening of each of the connection holes and prevent external contaminants from entering the culture well through the connection holes. The protective cover plate is made of transparent material.

4. A culture plate assembly for organoid electrophysiological data acquisition according to claim 2, characterized in that, The number of downward protruding connection holes provided on the culture well plate cover is 1 to 24.

5. A culture plate assembly for organoid electrophysiological data acquisition according to claim 1, characterized in that, The lead-out end is an electrode cable, and the acquisition end is a plurality of electrode contacts arrayed at the bottom of the culture well; the electrode cable passes through the through hole, and a sealing structure for sealing the gap between the through hole and the electrode cable is provided; the end of the electrode cable is detachably connected to the circuit board via a board-to-board connector.

6. A culture plate assembly for organoid electrophysiological data acquisition according to claim 1, characterized in that, The number of electrode channels in the electrode layer is 2 to 1024.

7. A culture plate assembly for organoid electrophysiological data acquisition according to claim 5, characterized in that, The board-to-board connector includes a male connector at the end of the electrode cable and a female connector on the circuit board. The male connector and the female connector are inserted into each other to achieve a detachable electrical connection.

8. A culture plate assembly for organoid electrophysiological data acquisition according to claim 7, characterized in that, The circuit board includes multiple circuit modules that are configured one-to-one with the electrode layers in the bottom of the multiple culture wells. Each circuit module is electrically connected to the corresponding electrode layer and is used to receive and process the electrophysiological signals of the organoid in the corresponding culture well.

9. A culture plate assembly for organoid electrophysiological data acquisition according to claim 8, characterized in that, The circuit board is a single-board main bearing circuit board, and multiple circuit modules are integrated on the single-board main bearing circuit board.

10. A culture plate assembly for organoid electrophysiological data acquisition according to claim 3, characterized in that, The protective cover plate has mounting holes at its four corners, the culture well plate cover has through holes at its four corners, and the circuit board has through holes at its four corners. The outer casing contains four hollow positioning posts. The mounting holes on the protective cover plate, the through holes on the culture well plate cover, the through holes on the circuit board, and the four hollow positioning posts have the same center distance. The outer diameter of each hollow positioning post is smaller than the inner diameter of the through holes on the circuit board. The four corner through holes of the circuit board are fitted onto the outside of the hollow positioning posts, positioning the circuit board inside the outer casing. The fasteners pass through the mounting holes of the protective cover and the through holes of the culture well plate cover in sequence, and then extend into the interior of the hollow positioning column to fix the protective cover, the culture well plate cover, the circuit board and the outer shell.

Images