A strain preservation tube for long-term preservation of anaerobic bacteria

Abstract

The utility model relates to a kind of strain preservation tubes for long-term preservation of anaerobic bacteria, it includes: tube body, for accommodating porcelain bead and preservation liquid;Its top opening is provided with lower thread;Tube cover, the bottom opening is connected by upper thread with the lower thread of the tube body and forms threaded connection, and its top opening is provided with buckle type lid;The space enclosed by the tube cover and the buckle type lid contains sheet-shaped deoxidizer, and the space is communicated with the inside of the tube body.Compared with prior art, the strain preservation tube of the utility model can better preserve anaerobic bacteria, and the replaceable deoxidizer can better maintain the anaerobic environment in the strain tube, improving the success rate of anaerobic bacteria recovery.

Description

TECHNICAL FIELD

[0001] The utility model relates to a send out device technical field, concretely relates to a kind of bacteria preservation tube for long-term preservation of anaerobic bacteria. BACKGROUND

[0002] Anaerobic bacteria are the pathogens of many infectious diseases, such as deep wounds, abscesses, oral infections, etc. They can cause infections alone or with other aerobic bacteria, leading to tissue necrosis and abscess formation. Intestinal anaerobes dominated by Bacteroides fragilis and other Bacteroides are commonly found in infections caused by the distal gastrointestinal tract. In particular, for patients with severe abdominal infections, drug selection usually covers most anaerobes.

[0003] Anaerobic bacteria have high nutritional requirements and are difficult to preserve. Current preservation methods include defiberization blood preservation, skimmed milk preservation, and gelatin sheet drying. However, these methods are complex and require certain equipment, making them unsuitable for widespread use. The most important difficulty in preserving anaerobic bacteria is maintaining an anaerobic environment. The above methods are not particularly effective in maintaining an anaerobic environment. Generally, after preservation, anaerobic bacteria are repeatedly frozen and thawed for recovery. Maintaining an anaerobic environment after recovery is even more difficult and critical for anaerobic bacteria preservation.

[0004] Bacteria preservation tubes are containers for preserving bacteria in laboratories, typically made of polypropylene (PP) material, which is resistant to low temperatures and can withstand -80℃ preservation conditions. Depending on the properties of the microorganisms to be preserved, different culture media or preservation materials are used in the bacteria preservation tube. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a bacteria preservation tube for long-term preservation of anaerobic bacteria, which can maintain a stable anaerobic environment inside.

[0006] The utility model can be realized by the following technical solutions: a bacteria preservation tube for long-term preservation of anaerobic bacteria, comprising: A tube body for containing ceramic beads and preservation liquid; a lower thread is provided at the top opening; A tube cover with a bottom opening connected to the lower thread of the tube body through an upper thread, and a buckle-type lid provided at the top opening; The space enclosed by the tube cover and the buckle-type lid contains a sheet-shaped deoxidizer, and the space is in communication with the interior of the tube body.

[0007] The utility model further improves the lower thread as an external thread, and the upper thread as an internal thread. A further improvement of this utility model is that the internal space of the tube cap is cylindrical, and the inner diameter of the cylindrical internal space is larger than the inner diameter of the tube body; when the tube body and the tube cap are connected by a threaded connection of a lower thread and an upper thread, the top of the tube body is accommodated in the bottom of the tube cap, and the top edge of the tube body restricts the vertical position of the flake deoxidizer to prevent the flake deoxidizer from falling into the tube body.

[0008] A further improvement of this invention is that the shape of the sheet-like deoxidizer includes circular and elliptical shapes; When the sheet-like deoxidizer is circular, its diameter is greater than the inner diameter of the tube body and smaller than the inner diameter of the internal space of the tube cap; When the flake-shaped deoxidizer is elliptical, its minor axis is longer than the inner diameter of the tube body but shorter than the inner diameter of the inner space of the tube cap.

[0009] A further improvement of this invention is that the ceramic bead has a porous structure.

[0010] A further improvement of this invention is that the liquid level of the preservation solution inside the tube is higher than the top of the ceramic bead.

[0011] A further improvement of this utility model is that one side of the snap-on cap is connected to the tube cap by a flexible connection structure; the bottom of the snap-on cap is provided with a snap-on structure that is adapted to the top edge of the tube cap.

[0012] A further improvement of this utility model is that the bottom of the tube is a flat surface.

[0013] Compared with the prior art, the present invention has the following advantages: 1. The preservation tube of this utility model uses a tablet-shaped replaceable deoxygenating agent product, which can quickly re-establish an anaerobic environment after one revival of anaerobic bacteria.

[0014] 2. The size of the flake deoxidizer is designed according to the pipe cap, so that it can be placed sideways inside the pipe cap and will not fall out.

[0015] 3. The top of the pipe cap has a snap-on design for easy replacement of the deoxidizer.

[0016] 4. The connection between the pipe cap and the pipe body is threaded to enhance sealing. Attached Figure Description

[0017] Figure 1 This is a rendering of the storage tube of this utility model; Figure 2 This is a completed diagram of the present invention, showing the inoculum being loaded and the cap screwed on. Figure 3This image shows the effect of opening the snap-on cap when replacing the deoxidizer. Detailed Implementation

[0018] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The following embodiments are implemented based on the technical solution of the present invention, providing detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.

[0019] like Figure 1 As shown, this utility model provides a bacterial culture preservation tube for long-term preservation of anaerobic bacteria, comprising: a tube body 8, a tube cap 7, and a snap-on cap 3 with an opening at the top of the tube cap 7. The tube body 8 contains ceramic beads 6 and a preservation solution 5, and its top opening has a downward thread 4. The bottom opening of the tube cap 7 is threadedly connected to the downward thread 4 of the tube body 8 via an upward thread 2, and its top opening has a snap-on cap 3. The space formed by the tube cap 7 and the snap-on cap 3 contains a sheet-like oxygen absorber 1, and this space is in communication with the interior of the tube body 8. The sheet-like oxygen absorber 1 in the tube cap 7 can absorb oxygen inside the tube body 8 to create an anaerobic environment. The sheet-like oxygen absorber 1 can be replaced by opening the snap-on cap 3. The bottom of the tube body 8 is flat, facilitating stable placement of the bacterial culture preservation tube on a table or similar surface.

[0020] In some embodiments, the lower thread 4 is an external thread, and the upper thread 2 is an internal thread. The internal space of the cap 7 is cylindrical, and the inner diameter of the cylindrical internal space is larger than the inner diameter of the tube body 8. When the tube body 8 and the cap 7 are threadedly connected by the lower thread 4 and the upper thread 2, the top of the tube body 8 is accommodated in the bottom of the cap 7, and the top edge of the tube body 8 restricts the vertical position of the flake deoxidizer 1, preventing the flake deoxidizer 1 from falling into the tube body 8. The threaded connection between the cap 7 and the tube body 8 allows the interior of the tube body 8 to remain sealed, preventing air from entering.

[0021] The shape of the flake-shaped deoxidizer 1 includes round and oval shapes.

[0022] When the flake-shaped deoxidizer 1 is circular, its diameter is larger than the inner diameter of the tube body 8 but smaller than the inner diameter of the internal space of the tube cap 7. This prevents the flake-shaped deoxidizer 1 from falling into the tube body 8.

[0023] When the sheet-like deoxidizer 1 is elliptical in shape, its minor axis is greater than the inner diameter of the tube body 8 but smaller than the inner diameter of the internal space of the tube cap 7. When the elliptical sheet-like deoxidizer 1 is placed inside the internal space of the tube cap 7, its orientation is tilted, as... Figure 2 As shown. This tilted posture makes it easier for the user to remove the sheet-like deoxidizer 1 from the tube cap 7.

[0024] In this embodiment, the tablet-shaped oxygen absorber 1 is a commercially available product used to absorb oxygen and slow down oxidation, effectively inhibiting the growth of mold and aerobic bacteria. The tablet-shaped oxygen absorber 1 can be an existing oxygen absorber composed of iron powder, copper powder and ammonium chloride, activated carbon, zinc, aluminum, and iron, etc. Its components meet the following conditions: 1. Safety, ensuring no adverse consequences even if accidentally ingested; 2. Moderate oxygen absorption rate. Too fast an absorption rate may cause a heating reaction, while too slow an absorption rate will be ineffective; 3. No toxic substances should be produced; 4. Stable performance, typically with a shelf life of at least 6 months; 5. Inexpensive; 6. Appropriate size for easy use. The oxygen absorber selected in this embodiment has no bactericidal effect on anaerobic bacteria and does not pollute the laboratory environment. Tablet-type oxygen absorbers are selected based on the size of the bacterial culture tubes and convenience, with individual packaging for easy replacement.

[0025] In this embodiment, the ceramic beads 6 have a porous structure. The level of the preservation solution 5 inside the tube 8 is higher than the top of the ceramic beads 6. The ceramic beads 6 and preservation solution 5 inside the tube 8 can better preserve anaerobic bacteria and reduce the impact of ice crystals on the bacterial strain during cryopreservation. The ceramic beads 6 do not require acidification treatment, are uniform in size and regular in shape, and are used for bacterial adsorption and preservation. Each bead has a roughly the same loading rate, resulting in a high colony loading rate. Using the ceramic bead 6 method to preserve bacteria is convenient for both retrieval and preservation (retrieval, revival, and preservation of strains takes only 1 minute), and is widely used for preserving common bacteria, fastidious bacteria, and fungi such as molds and yeasts. It can be used 25 times (one bead each time) within the preservation period.

[0026] In this embodiment, as Figure 2 , Figure 3 The snap-on cap 3 shown is connected to the tube cap 7 on one side via a flexible connection structure; the bottom of the snap-on cap 3 has a snap-on structure that fits the top edge of the tube cap 7. When replacing the sheet oxygen absorber 1, open the snap-on cap 3, remove the sheet oxygen absorber 1, and insert a new sheet oxygen absorber 1. After replacement, close the snap-on cap 3. After reviving the anaerobic bacteria, the anaerobic environment can be quickly re-established by replacing the sheet oxygen absorber 1.

[0027] The process of preserving bacterial cultures using the culture preservation tube of this embodiment includes: inoculating the bacteria to be preserved onto thioglycolate medium (anaerobic medium), scraping the bacteria to be preserved with a disposable sterile cotton swab and placing it in the culture preservation tube. The tube is shaken to mix thoroughly, ensuring that the ceramic beads 6 are evenly coated with the bacterial solution. The lower thread 4 and upper thread 2 are tightened, and then a sheet-like oxygen absorber 1 is added. The diameter of the sheet-like oxygen absorber 1 is designed according to the culture preservation tube, fitting perfectly sideways inside the tube cap 7 to prevent it from falling out. Finally, the snap-on cap 3 is closed, and the culture tube is placed in an ultra-low temperature freezer at -80°C for long-term storage. Figure 2 .

[0028] like Figure 3As shown, in this embodiment, when bacterial resuscitation is required, the bacterial culture preservation tube is opened, and a ceramic bead 6 is taken out with sterile forceps, rolled back and forth on the anaerobic culture medium, and embedded in the agar. After use, the sheet-like oxygen absorber 1 is replaced to maintain the anaerobic environment of the bacterial culture preservation tube, and the bacterial culture preservation tube is kept in an ultra-low temperature freezer at -80°C for long-term storage. Figure 3 .

[0029] The above description of the embodiments is provided to enable those skilled in the art to understand and use the present invention. It will be apparent to those skilled in the art that various modifications can be easily made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the protection scope of the present invention.

Claims

1. A bacterial culture preservation tube for long-term preservation of anaerobic bacteria, characterized in that, include: The tube (8) is used to hold the ceramic beads (6) and the preservation solution (5); Its top opening is provided with a down thread (4); The pipe cap (7) has a bottom opening that is threadedly connected to the lower thread (4) of the pipe body (8) through an upper thread (2), and a snap-on cap (3) is provided at its top opening. The space formed by the tube cap (7) and the snap-on cap (3) contains the sheet-like deoxidizer (1), and the space is in communication with the interior of the tube body (8); The internal space of the tube cap (7) is cylindrical, and the inner diameter of the cylindrical internal space is larger than the inner diameter of the tube body (8). When the tube body (8) and the tube cap (7) are connected by a lower thread (4) and an upper thread (2), the top of the tube body (8) is accommodated at the bottom of the tube cap (7), and the top edge of the tube body (8) restricts the vertical position of the flake deoxidizer (1) to prevent the flake deoxidizer (1) from falling into the tube body (8). The shape of the sheet-like deoxidizer (1) includes round and elliptical shapes; When the shape of the sheet-like deoxidizer (1) is circular, its diameter is greater than the inner diameter of the tube body (8) and smaller than the inner diameter of the internal space of the tube cap (7); When the shape of the flake deoxidizer (1) is elliptical, its minor axis length is greater than the inner diameter of the tube body (8) and smaller than the inner diameter of the internal space of the tube cap (7).

2. The bacterial culture preservation tube for long-term preservation of anaerobic bacteria according to claim 1, characterized in that, The lower thread (4) is an external thread, and the upper thread (2) is an internal thread.

3. The bacterial culture preservation tube for long-term preservation of anaerobic bacteria according to claim 1, characterized in that, The ceramic bead (6) has a porous structure.

4. A bacterial culture preservation tube for long-term preservation of anaerobic bacteria according to claim 1, characterized in that, Inside the tube (8), the level of the preservation liquid (5) is higher than the top of the ceramic bead (6).

5. A bacterial culture preservation tube for long-term preservation of anaerobic bacteria according to claim 1, characterized in that, One side of the snap-on cap (3) is connected to the tube cap (7) by a flexible connection structure; the bottom of the snap-on cap (3) is provided with a snap-on structure that is adapted to the top edge of the tube cap (7).

6. A bacterial culture preservation tube for long-term preservation of anaerobic bacteria according to claim 1, characterized in that, The bottom of the tube (8) is flat.

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