A strain and culture medium mixing device

Abstract

The utility model discloses a kind of strain and culture medium mixing device, it is related to mixing equipment technical field, to solve the problem of existing mixing equipment not completely, mixing uneven and easily contaminated. Technical scheme points are as follows: device includes conical mixing barrel, installation cover and double helix stirring component;Conical mixing barrel has inner bag, its outer wall is equipped with spiral partition, partition gap installs heating wire, outer side sealing connection shell, spiral waterway is formed in common, shell lower portion is equipped with water inlet pipe, upper portion is equipped with water outlet pipe;Installation cover is sealingly installed in mixing barrel top, its mounting seat fixed stirring component;Through heating wire heating, realize culture medium high-temperature sterilization, then pass through spiral waterway and cool, finally through stirring component, realize the uniform mixing of strain and culture medium.The utility model is mainly used for the sterile, uniform mixing of edible mushroom liquid strain and culture medium.

Description

Technical Field

[0001] This utility model relates to the field of mixing equipment technology, and in particular to a device for mixing bacterial strains and culture media. Background Technology

[0002] As a prized edible fungus, deer antler mushroom boasts high nutritional and market value. However, its mycelial growth is relatively slow, and its resistance to contaminating microorganisms is weak, placing higher demands on production efficiency and stability. Traditional inoculation methods primarily rely on "spot inoculation" with solid spawn, where blocks of solid spawn are manually inoculated onto specific points on or within the culture medium. This method has inherent drawbacks: firstly, the mycelium must start from a limited inoculation point and slowly spread outwards, resulting in low production efficiency; secondly, the uneven age of mycelium within the substrate bag leads to uneven fruiting, cumbersome management, and difficulty in guaranteeing yield and quality.

[0003] To overcome the drawbacks of solid microbial cultures, liquid microbial culture technology has emerged. This technology prepares liquid microbial cultures rich in mycelial balls using fermenters, offering advantages such as uniform mycelial age and high viability. However, even with liquid microbial cultures, the mainstream inoculation methods are still largely limited to "injection" or "surface spraying," essentially remaining within a "point-to-surface" growth model and failing to fully realize the potential for uniform distribution of liquid microbial cultures. Furthermore, in large-scale production, existing mixing equipment struggles to achieve large-scale, efficient, and highly uniform mixing of liquid microbial cultures and culture media, resulting in problems such as mixing dead zones and a high risk of secondary contamination.

[0004] It is necessary to design a mixing device that can ensure that the inoculum and culture medium are mixed evenly while preventing secondary contamination. Utility Model Content

[0005] To address the above shortcomings, this utility model provides a device for mixing bacterial strains and culture media, which can sterilize the culture media before mixing the bacterial strains and culture media, ensuring uniform mixing of the bacterial strains and culture media during mixing and preventing secondary contamination.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A device for mixing microbial strains and culture media includes a conical mixing tank, a double-helix stirring component, and a mounting cover. The mounting cover includes a cover body and a mounting base, with the mounting base fixed to the upper part of the cover body. The cover body is sealed and installed on the upper part of the conical mixing tank. The double-helix stirring component is installed on the mounting base. The conical mixing tank includes an inner liner, an outer shell, a partition, a water inlet pipe, a water outlet pipe, and a heating wire. The inner liner is a conical partition that is spirally fixed to the outer wall of the inner liner. The heating wire is spirally installed between the gaps formed by the partition. The outer shell is sealed and installed on the outside of the partition, forming a spiral upward water channel with the partition and the inner liner. The water inlet pipe is located at the lower part of the outer shell and communicates with the water channel. The water outlet pipe is located at the upper part of the outer shell and communicates with the water channel. An electrical interface is also provided on the outer shell, which is sealed and electrically connected to the heating wire. In use, first place the culture medium into the conical mixing tank and heat it under standard sterilization conditions (e.g., 121°C, 1-2 hours) using a heating wire. This initial step removes contaminating microorganisms to prevent them from affecting subsequent inoculum growth. After sterilization, cool the culture medium to the mixing temperature by circulating water through the inlet pipe. Then, pour the prepared bacterial solution into the tank according to the specified ratio. Use a double-helix stirring device to evenly mix the bacterial solution and culture medium. Once mixed, the mixture can be bagged and placed in a suitable environment for inoculum growth. This process minimizes the intrusion of contaminating microorganisms, maintaining the purity of the inoculum to the greatest extent possible.

[0007] Preferably, the double-helix mixing component includes a motor, a shaft, and double helix blades. The motor is mounted on the upper part of the mounting base, the shaft is fixedly connected to the output shaft of the motor and passes through the mounting base into the conical mixing tank, and the double helix blades are fixedly connected to the shaft. The double helix blades here can refer to the double helix blades in patent number CN217989091U, which can achieve more uniform mixing.

[0008] Preferably, the mounting cover also includes water inlets, connecting water pipes, and nozzles. There are multiple water inlets, evenly distributed circumferentially on the upper part of the cover. The connecting water pipes are connected to the multiple water inlets. Multiple nozzles are installed below each water inlet on the cover. This nozzle configuration allows for cleaning of the inner wall of the mixing tank after mixing without opening the cover. Some nozzles point towards the inner wall, while others point towards the shaft and twin propeller blades.

[0009] Preferably, the cover also includes a thermo-pressure gauge, which is installed on the cover to monitor the temperature and pressure inside the conical mixing tank. At the same time, a vent valve is also provided on the cover to release pressure when the air pressure inside the conical mixing tank is too high.

[0010] Preferably, the cover also includes a feed inlet and a first sealing cover, the first sealing cover being detachably and sealingly installed on the feed inlet.

[0011] Preferably, it also includes a support frame, which includes a support base, support rods and anti-slip plates. The support base is funnel-shaped and matches the conical mixing tank. The support base is sleeved on the outside of the conical mixing tank. There are multiple support rods and they are circumferentially fixed to the outside of the support base. The anti-slip plates are fixed to the bottom of the support rods.

[0012] Preferably, it also includes a discharge cover, and the lower part of the inner liner of the conical mixing tank is provided with a discharge port, and the discharge cover is sealed and installed at the discharge port.

[0013] Preferably, it also includes a valve, which is installed on the connecting water pipe and connected to a water source at the other end. The water flow can be controlled by opening and closing the valve, thereby controlling the water pressure, which facilitates cleaning.

[0014] Preferably, the first sealing cap also includes a bacterial liquid inlet and a second sealing cap. The second sealing cap is detachably and sealed above the bacterial liquid inlet, creating a smaller bacterial liquid inlet to further prevent contamination by other microorganisms.

[0015] Preferably, the discharge cap includes a limiting plug and a handle. The handle has multiple parts that are uniformly fixed to the outside of the discharge cap in a circumferential direction. The limiting plug is fixed to the middle of the inside of the discharge cap. The limiting plug is used to prevent the culture medium from falling to the bottom and causing uneven mixing.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows: the device includes a conical mixing tank, a double-spiral stirring component, and a mounting cover. The mounting cover includes a cover body and a mounting base, with the mounting base fixed to the upper part of the cover body. The cover body is sealed and installed on the upper part of the conical mixing tank. The double-spiral stirring component is installed on the mounting base. The conical mixing tank includes an inner liner, an outer shell, a partition, a water inlet pipe, a water outlet pipe, and a heating wire. The inner liner has a conical partition that is spirally fixed to the outer wall of the inner liner. The heating wire is spirally installed between the gaps formed by the partition. The outer shell is sealed and installed on the outside of the partition, forming a spiral upward water channel with the partition and the inner liner. The conical mixing tank, through the spiral upward water channel formed by the spiral partition on the outer wall of the inner liner, the heating wire, and the outer shell, combined with the water inlet and outlet pipes, achieves efficient sterilization and rapid cooling of the culture medium, thereby completely eliminating contamination by miscellaneous bacteria before mixing, ensuring uniform mixing of the strains in a pure environment, and improving production efficiency and strain quality. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a cross-sectional view of the conical mixing hopper in this utility model; Figure 3 This is a schematic diagram of the structure of the double-helix stirring component in this utility model; Figure 4 This is an exploded view of the entire device in this utility model; Figure 5 This is a lower side view of the sealing cap in this utility model; Figure 6 This is a schematic diagram of the overall structure of the support frame in this utility model; Figure 7 This is a schematic diagram of the discharge cover in this utility model.

[0019] Reference numerals in the attached drawings: 1. Conical mixing tank; 101. Inner liner; 102. Outer shell; 103. Partition; 104. Heating wire; 105. Water inlet pipe; 106. Water outlet pipe; 107. Discharge port; 108. Electrical interface; 2. Mounting cover; 201. Cover body; 202. Water inlet; 203. Connecting water pipe; 204. Valve; 205. Temperature and pressure gauge; 206. Mounting base; 207. Feed inlet; 208. First sealing cover; 209. Bacterial liquid pouring inlet; 210. Second sealing cover; 211. Nozzle; 3. Support frame; 301. Support base; 302. Support rod; 303. Anti-slip plate; 4. Double helix stirring component; 401. Motor; 402. Shaft; 403. Double helix blades; 5. Discharge cover; 501. Limiting plug; 502. Handle. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0021] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0022] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances. Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0023] This utility model provides a specific embodiment of a device for mixing spawn and culture medium. This device is mainly used for the uniform mixing of liquid spawn and culture medium for edible fungi such as deer antler fungus, and also has sterilization, cooling, and anti-contamination functions; for example... Figures 1-7 As shown, the device includes a conical mixing tank 1, whose inner liner 101 has a conical structure for containing culture medium and inoculum. The conical design facilitates the collection and discharge of materials. A spiral baffle 103 is fixed to the outer wall of the inner liner 101, and the baffle 103 fits tightly against the outer wall of the inner liner 101, forming a spirally ascending channel. Heating wires 104 are spirally installed between the gaps formed by the baffles 103. The heating wires 104 are used for heating during the sterilization stage, providing high temperatures to kill contaminants in the culture medium. A shell 102 is sealed to the outside of the baffles 103. The shell 102, together with the baffles 103 and the inner liner 101, forms a sealed spirally ascending water channel, which allows water circulation for cooling the culture medium after sterilization. A water inlet pipe 105 is provided at the lower part of the shell 102, communicating with the water channel for introducing cooling water; a water outlet pipe 106 is provided at the upper part of the shell 102, also communicating with the water channel for discharging the cooled water. In addition, an electrical interface 108 is sealed on the outer casing 102. The electrical interface 108 is electrically connected to the heating wire 104 for external power supply to power the heating wire 104, thereby achieving controllable heating.

[0024] A sealing cover 2 is installed on the upper part of the conical mixing tank 1. The sealing cover 2 includes a cover body 201 and a mounting seat 206 fixed to the upper part of the cover body 201. The cover body 201 is sealed to the opening of the conical mixing tank 1 by a sealing strip and a nut to prevent external contaminants from entering. The mounting seat 206 is used to support and fix the double helix stirring component 4. Multiple water inlets 202 are evenly arranged circumferentially on the upper part of the cover body 201. Each water inlet 202 has a corresponding nozzle 211 installed at the bottom, facing the inside of the conical mixing tank 1, for cleaning the inner wall and stirring component after mixing. The multiple water inlets 202 are interconnected by a connecting water pipe 203. A valve 204 is installed on the connecting water pipe 203 to control the water flow rate and pressure, facilitating the adjustment of the cleaning effect. A thermo-pressure gauge 205 is also installed on the lid 201, extending into the conical mixing tank 1 to monitor the temperature and pressure inside the tank in real time. A vent valve is also integrated into the lid 201 to automatically release pressure when the internal pressure is too high, ensuring operational safety. An inlet 207 is provided on the lid 201, and a first sealing cap 208 is detachably and threadedly installed on the inlet 207. The first sealing cap 208 is opened when adding culture medium and remains sealed during mixing. A bacterial solution inlet 209 is also provided on the first sealing cap 208, and a second sealing cap 210 is detachably and threadedly installed on the inlet 209. The second sealing cap 210 is opened when adding bacterial solution, and its small-diameter design further reduces the risk of contamination by other microorganisms.

[0025] The double-helix mixing component 4 is mounted on the mounting base 206 and includes a motor 401, a shaft 402, and double helix blades 403. The motor 401 is fixedly mounted on the upper part of the mounting base 206, and its output shaft is fixedly connected to the shaft 402. The shaft 402 passes downward through the mounting base 206 and extends into the conical mixing tank 1. The double helix blades 403 are fixedly connected to the shaft 402. The double helix blades 403 adopt a symmetrical helical design. The double helix blades here can refer to the double helix blades in patent number CN217989091U. They can more evenly complete the mixing and can push the material up and down and radially during mixing to ensure that the inoculum and culture medium are evenly mixed in the conical space and avoid dead corners.

[0026] To stably support the entire device, a support frame 3 is also provided. The support frame 3 includes a support base 301, support rods 302, and anti-slip plate 303. The support base 301 has a funnel-shaped structure that matches the shape of the conical mixing tank 1 and is fitted onto the outside of the conical mixing tank 1 to provide uniform support force. Multiple support rods 302 are circumferentially fixed to the outside of the support base 301. Anti-slip plate 303 is fixed to the bottom of the support rods 302. The anti-slip plate 303 increases the friction with the ground and prevents the device from sliding or tilting during operation.

[0027] The conical mixing tank 1 has a discharge port 107 at the lower part of its inner liner 101 for discharging materials after mixing. A discharge cover 5 is sealed at the discharge port 107, and the discharge cover 5 includes a limiting plug 501 and a handle 502. The limiting plug 501 is fixed to the middle of the inner side of the discharge cover 5. When the discharge cover 5 is closed, the limiting plug 501 extends into the discharge port 107 to prevent the culture medium from settling at the bottom and ensure uniform mixing. Multiple handles 502 are evenly fixed to the outer side of the discharge cover 5 in a circumferential direction for easy manual opening and closing.

[0028] In specific operation, firstly, open the first sealing cap 208 and add the culture medium into the conical mixing tank 1 through the feed inlet 207, then seal the cap 201. Power is supplied through the electrical interface 108, and the heating wire 104 directly heats the inner tank 101 to sterilize the culture medium under standard sterilization conditions (e.g., 121°C, 1-2 hours). After sterilization, cooling water is introduced through the water inlet pipe 105. The water flows upward along the spiral waterway, carrying away heat and cooling the culture medium to a suitable mixing temperature. Subsequently, open the second sealing cap 210 and inject liquid inoculum through the bacterial liquid pouring inlet 209 in proportion. Start the motor 401, and the double spiral stirring component 4 operates. The double spiral blades 403 drive the material to mix, ensuring uniform distribution of the inoculum. After mixing, open the discharge cap 5, and the material is discharged from the discharge port 107 for bagging. Finally, cleaning water is introduced through the connecting water pipe 203 and the nozzle 211 to clean the inner wall of the tank and the stirring component. The valve 204 controls the water flow to improve cleaning efficiency. Throughout the process, the temperature and pressure gauge 205 monitors environmental parameters in real time, and the vent valve ensures safety, thus achieving efficient, uniform, and low-pollution mixing operation.

[0029] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A device for mixing microbial strains and culture media, comprising a conical mixing tank (1), a double-helix stirring component (4), and a mounting cover (2), wherein the mounting cover (2) comprises a cover body (201) and a mounting base (206), the mounting base (206) being fixedly connected to the upper part of the cover body (201), the cover body (201) being sealed and installed on the upper part of the conical mixing tank (1), and the double-helix stirring component (4) being mounted on the mounting base (206), characterized in that, The conical mixing tank (1) includes an inner liner (101), an outer shell (102), a partition (103), a water inlet pipe (105), a water outlet pipe (106), and a heating wire (104). The inner liner (101) is conical, and the partition (103) is spirally fixed to the outer wall of the inner liner (101). The heating wire (104) is spirally installed between the gaps formed by the partition (103). The outer shell (102) is sealed and installed on the outside of the partition (103). The partition (103) and the inner liner (101) form a spiral upward water passage. The water inlet pipe (105) is located at the lower part of the outer shell (102) and communicates with the water passage. The water outlet pipe (106) is located at the upper part of the outer shell (102) and communicates with the water passage. An electrical interface (108) is also provided on the outer shell (102). The electrical interface (108) is sealed on the outer shell (102) and electrically connected to the heating wire (104).

2. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, The double helix stirring component (4) includes a motor (401), a shaft (402), and double helix blades (403). The motor (401) is mounted on the upper part of the mounting base (206). The shaft (402) is fixed to the output shaft of the motor (401) and passes through the mounting base (206) into the conical mixing tank (1). The double helix blades (403) are fixed to the shaft (402).

3. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, The mounting cover (2) also includes a water inlet (202), a connecting water pipe (203), and a nozzle (211). There are multiple water inlets (202), which are evenly arranged on the upper part of the cover body (201) in a circumferential direction. The connecting water pipe (203) is connected to the multiple water inlets (202). There are multiple nozzles (211) and they are installed on the lower part of each water inlet (202) of the cover body (201).

4. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, The mounting cover (2) also includes a thermo-pressure gauge (205), which is mounted on the cover (201) to monitor the temperature and pressure inside the conical mixing tank (1).

5. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, The cover (201) also includes a feed inlet (207) and a first sealing cover (208), the first sealing cover (208) being detachably and sealingly installed on the feed inlet (207).

6. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, It also includes a support frame (3), which includes a support base (301), a support rod (302) and an anti-slip plate (303). The support base (301) is funnel-shaped and matches the conical mixing tank (1). The support base (301) is sleeved on the outside of the conical mixing tank (1). There are multiple support rods (302) and they are circumferentially fixed to the outside of the support base (301). The anti-slip plate (303) is fixed to the bottom of the support rod (302).

7. The apparatus for mixing microbial strains and culture medium according to claim 1, characterized in that, It also includes a discharge cover (5), and the lower part of the inner liner (101) of the conical mixing tank (1) is provided with a discharge port (107), and the discharge cover (5) is sealed and installed at the discharge port (107).

8. The apparatus for mixing microbial strains and culture medium according to claim 3, characterized in that, It also includes a valve (204) which is installed on the connecting water pipe (203).

9. The apparatus for mixing microbial strains and culture medium according to claim 5, characterized in that, The first sealing cap (208) also includes a bacterial liquid inlet (209) and a second sealing cap (210), the second sealing cap (210) being detachably and sealingly installed on the upper part of the bacterial liquid inlet (209).

10. The apparatus for mixing microbial strains and culture medium according to claim 7, characterized in that, The discharge cover (5) includes a limiting plug (501) and a handle (502). The handle (502) has multiple parts and is uniformly fixed to the outside of the discharge cover (5) in a circumferential direction. The limiting plug (501) is fixed to the middle of the inside of the discharge cover (5). The limiting plug (501) is used to prevent the culture medium from falling to the bottom and causing uneven mixing.

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