Ultrasonic pre-hemolysis assembly

Abstract

The utility model discloses an ultrasonic wave pre -lysis blood -preliminary dissolving assembly relates to sample pre -lysis processing technical field, including front cover, the front cover is integrally fixed with tool head on, and tool head top is equipped with flowing inner chamber, and the inner wall of flowing inner chamber is treated through mirror polishing, one side of flowing inner chamber is sample inflow end, and the other side of flowing inner chamber is sample outflow end. The application provides an ultrasonic wave pre -lysis blood -preliminary dissolving assembly, adopts ultrasonic transducer front cover and tool head integrated structure, can pass through the tool head of front cover and transmit ultrasonic energy to the sample that needs hemolysis stably, advantage one is, the front cover and tool head of ultrasonic transducer are integrated, and the coupling performance of this mode is good, after ultrasonic energy is sent from ultrasonic transducer, when ultrasonic wave is evenly transmitted to reach tool head through integrated front cover, the working surface ultrasonic energy is even, and the situation that partial cell is not broken does not appear.

Description

Technical Field

[0001] This utility model relates to the field of sample pre-lysis processing technology, specifically to an ultrasonic pre-hemolysis component. Background Technology

[0002] In medical settings, red blood cell prelysis (hemolysis) is commonly used in laboratories. The core advantages of ultrasonic disruption are the absence of chemical pollution and wide applicability. It destroys cells solely through the physical action of ultrasound, without adding any chemical reagents, thus avoiding contamination of subsequently extracted bioactive substances such as proteins and nucleic acids.

[0003] The core drawbacks of using ultrasonic disruption for sample pre-lysis are: first, the distribution of ultrasonic energy is limited, which may result in local over-disruption or some cells remaining undisturbed, affecting the reproducibility of subsequent experiments; second, it requires the use of containers made of specific materials (such as quartz or specific plastics), and the sample volume cannot be too large, otherwise it will weaken the ultrasonic energy and limit large-scale sample processing. Utility Model Content

[0004] The purpose of this invention is to provide an ultrasonic pre-hemolysis component to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: an ultrasonic pre-hemolysis component, including a front cover plate, on which a tool head is integrally fixed, and the top of the tool head is provided with a flow cavity, and the inner wall of the flow cavity is mirror polished.

[0006] Furthermore, one side of the flow cavity is the sample inflow end, and the other side of the flow cavity is the sample outflow end.

[0007] Furthermore, a flexible tube is connected to the sample inflow end of the flow cavity, and the flexible tube is connected to the liquid outlet end of the squeeze pump.

[0008] Furthermore, the front cover plate is mounted on the ceramic sheet, and a rear cover plate is mounted below the ceramic sheet. The rear cover plate, the ceramic sheet, and the front cover plate are fastened together by connecting bolts to form a sandwich-type piezoelectric ultrasonic transducer.

[0009] Compared with the prior art, the present invention has the following beneficial effects: 1. This utility model adopts an integrated structure of the ultrasonic transducer front cover and the tool head, which can stably transmit ultrasonic energy to the sample requiring hemolysis through the tool head of the front cover. The advantages are: first, the front cover and the tool head of the ultrasonic transducer are integrated, which has good coupling performance. After the ultrasonic energy is emitted from the ultrasonic transducer, it is uniformly transmitted to the tool head through the integrated front cover, and the ultrasonic energy on the working surface is uniform, without local excessive fragmentation or partial cell fragmentation; second, by using flowing samples for ultrasound, sample pretreatment can be performed in batches; third, the integrated front cover structure avoids the problem of the front cover and the tool head being combined, which can easily cause the parts to loosen during ultrasonic vibration and affect the service life of the product. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model; Figure 2 This is a schematic diagram of the split structure of the ultrasonic transducer of this utility model; Figure 3 This is a schematic cross-sectional view of the ultrasonic transducer of this utility model.

[0011] In the diagram: 1. Front cover plate; 2. Tool head; 3. Flow chamber; 4. Hose; 5. Extrusion pump; 6. Ceramic disc; 7. Rear cover plate; 8. Connecting bolts. Detailed Implementation

[0012] The embodiments of this utility model will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of this utility model.

[0013] like Figures 1 to 3 As shown, an ultrasonic pre-hemolysis component includes a front cover plate 1, a tool head 2 integrally fixed on the front cover plate 1, and a flow cavity 3 at the top of the tool head 2. The inner wall of the flow cavity 3 is mirror polished. One side of the flow cavity 3 is the sample inlet end, and the other side of the flow cavity 3 is the sample outlet end. A hose 4 is connected to the sample inlet end of the flow cavity 3, and the hose 4 is connected to the liquid outlet end of the squeeze pump 5. The front cover plate 1 is installed on a ceramic plate 6, and a rear cover plate 7 is installed below the ceramic plate 6. The rear cover plate 7, the ceramic plate 6, and the front cover plate 1 are fastened together by connecting bolts 8 to form a sandwich piezoelectric ultrasonic transducer. The specific operation is as follows: This application adopts an integrated structure of the ultrasonic transducer front cover plate 1 and the tool head 2. The ultrasonic energy can be stably transmitted to the sample that needs to be hemolyzed through the tool head 2 of the front cover plate 1. The integrated structure of the front cover plate 1 and the tool head 2 of the ultrasonic transducer has good coupling performance. After the ultrasonic energy is emitted from the ultrasonic transducer, it is uniformly transmitted to the tool head 2 through the integrated front cover plate 1. The ultrasonic energy on the working surface is uniform, and there will be no local over-fragmentation or partial cell undism. By performing ultrasound on the flowing sample, sample pretreatment can be carried out in batches. The integrated front cover plate 1 structure avoids the problem that when the front cover plate 1 and the tool head 2 are combined, the combined structure is prone to loosening of the parts during ultrasonic vibration, which affects the service life of the product.

[0014] Working principle: Connect the connecting hose 4 to both ends of the tool head 2 of the integrated ultrasonic hemolysis component. One end is the sample inlet end and the other end is the sample outlet end. The sample that needs to be pre-hemolyzed is driven by the squeeze pump 5 and flows through the flow cavity 3 of the tool head 2 according to the set parameters (mainly the flow rate). The inner wall of the flow cavity 3 is mirror polished, so there is almost no inner wall resistance to hinder the flow of the sample. Start the ultrasound and squeeze pump 5 to start the sample pre-hemolysis work.

[0015] The embodiments of this utility model are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the utility model to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical applications of this utility model, and to enable those skilled in the art to understand this utility model and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. An ultrasonic pre-hemolysis assembly, comprising a front cover plate (1), characterized in that, A tool head (2) is integrally fixed on the front cover plate (1), and the top of the tool head (2) is provided with a flow cavity (3), and the inner wall of the flow cavity (3) is mirror polished.

2. The ultrasonic pre-hemolysis component according to claim 1, characterized in that, One side of the flow cavity (3) is the sample inflow end, and the other side of the flow cavity (3) is the sample outflow end.

3. The ultrasonic pre-hemolysis component according to claim 2, characterized in that, A flexible tube (4) is connected to the sample inflow end of the flow chamber (3), and the flexible tube (4) is connected to the liquid outlet end of the squeeze pump (5).

4. The ultrasonic pre-hemolysis component according to claim 3, characterized in that, The front cover plate (1) is installed on the ceramic plate (6), and the rear cover plate (7) is installed below the ceramic plate (6). The rear cover plate (7), the ceramic plate (6) and the front cover plate (1) are fastened together by connecting bolts (8) to form a sandwich piezoelectric ultrasonic transducer.

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