An auxiliary calibration chip for nucleic acid mass spectrometry data reading

Abstract

The utility model relates to nucleic acid detection and mass spectrum analysis auxiliary equipment technical field discloses a kind of for nucleic acid mass spectrum data reading auxiliary calibration sheet, including reinforcing plate, the top of the reinforcing plate is fixedly connected with substrate assembly, the top of the substrate assembly is fixedly connected with protective film, the bottom of the reinforcing plate is fixedly connected with magnetic attraction plate, the inside of protective film, substrate assembly, reinforcing plate and magnetic attraction plate is evenly opened with locating hole, the top of the protective film one side one end is fixedly connected with first molecular weight label, the top of the protective film one side middle part is fixedly connected with second molecular weight label, the top of the protective film one side other end is fixedly connected with third molecular weight label.In the utility model, substrate body sets low, middle, high molecular weight standard point, covers nucleic acid mass spectrum common detection range, can carry out comprehensive calibration to instrument signal intensity and molecular weight accuracy;Magnetic attraction plate cooperates locating hole, quickly adsorbs fixed and accurate alignment stage.

Description

Technical Field

[0001] This utility model relates to the technical field of auxiliary equipment for nucleic acid detection and mass spectrometry analysis, and in particular to an auxiliary calibration strip for reading nucleic acid mass spectrometry data. Background Technology

[0002] Nucleic acid mass spectrometry is an analytical technique that detects the mass signal of ionized nucleic acid molecules to achieve genotyping, mutation detection, and other functions. It is widely used in clinical diagnosis, drug development, and other fields. Its detection accuracy directly determines the reliability of the results. However, during long-term use, the stability of the ion source and the accuracy of the mass axis can be affected, leading to data deviation. Auxiliary calibration strips provide standard nucleic acid fragments with known molecular weights. They are inserted into the instrument for calibration before detection. They can correct mass axis deviations, optimize signal response, and ensure that the instrument is always in a precise state. They are key auxiliary consumables for ensuring the accuracy and stability of nucleic acid mass spectrometry detection results.

[0003] Existing auxiliary calibration plates mostly contain only a single or a few molecular weight standard points, with a narrow coverage range, making it difficult to adapt to the calibration needs of low, medium and high molecular weight fragments in nucleic acid mass spectrometry detection, which can easily lead to accuracy deviations in some detection intervals. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides an auxiliary calibration strip for nucleic acid mass spectrometry data reading, aiming to improve the problem that traditional calibration strips have a narrow standard point coverage range and are difficult to adapt to the needs of multi-molecular weight detection.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an auxiliary calibration sheet for nucleic acid mass spectrometry data reading, comprising a reinforcing plate, a substrate assembly fixedly connected to the top of the reinforcing plate, a protective film fixedly connected to the top of the substrate assembly, and a magnetic suction plate fixedly connected to the bottom of the reinforcing plate. Positioning holes are uniformly formed inside the protective film, the substrate assembly, the reinforcing plate, and the magnetic suction plate. A first molecular weight label is fixedly connected to one end of the top side of the protective film, a second molecular weight label is fixedly connected to the middle of the top side of the protective film, a third molecular weight label is fixedly connected to the other end of the top side of the protective film, and a QR code is provided on one end of the other top side of the protective film.

[0006] By adopting the above technical solution, the substrate body is set with low, medium and high molecular weight standard points, covering the common detection range of nucleic acid mass spectrometry, which can comprehensively calibrate the instrument signal intensity and molecular weight accuracy; the magnetic plate, together with the positioning hole, can quickly adsorb and fix and accurately align the stage, avoiding the detection deviation caused by the displacement of the calibration plate. This solves the problems of the narrow coverage of standard points of traditional calibration plates, which makes it difficult to adapt to the needs of multi-molecular weight detection, and the fact that inaccurate positioning can easily affect the calibration accuracy.

[0007] Preferably, the substrate assembly includes a substrate body, with low molecular weight standard points uniformly disposed at one end of the top side of the substrate body, and medium molecular weight standard points uniformly disposed at the middle of the top side of the substrate body.

[0008] Preferably, high molecular weight standard points are uniformly arranged on one side of the top of the substrate body and the other end, and the top of the substrate body is fixedly connected to the bottom of the protective film.

[0009] Preferably, the positioning holes are uniformly formed inside the substrate body.

[0010] Preferably, a text label is fixedly connected to the center of the other side of the protective film.

[0011] Preferably, an indicator arrow is fixedly connected to the other end of the other side of the protective film.

[0012] Preferably, the protective film is made of transparent polyethylene terephthalate film with a thickness of 50-100 μm.

[0013] Preferably, the reinforcing plate is made of lightweight aluminum alloy with a thickness of 1-2 mm.

[0014] This utility model has the following beneficial effects:

[0015] 1. In this utility model, the substrate body is set with low, medium and high molecular weight standard points, covering the common detection range of nucleic acid mass spectrometry, which can comprehensively calibrate the instrument signal intensity and molecular weight accuracy; the magnetic plate, together with the positioning hole, can quickly adsorb and fix and accurately align the stage, avoiding the detection deviation caused by the displacement of the calibration sheet, and solving the problem that the standard point coverage of traditional calibration sheets is narrow and difficult to adapt to the needs of multi-molecular weight detection.

[0016] 2. In this utility model, the transparent protective film prevents the standard points from being contaminated and worn, and the lightweight aluminum alloy reinforcing plate prevents the calibration sheet from bending and deforming, thus jointly ensuring the stability of the standard points and the service life of the calibration sheet; the QR code stores information such as specifications and batches, which can be retrieved by scanning; the indicator arrow clearly indicates the installation direction; and the text label provides instructions for use, greatly simplifying the operation process. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural schematic diagram of an auxiliary calibration strip for nucleic acid mass spectrometry data reading proposed in this utility model;

[0018] Figure 2 This is a partial structural diagram of the reinforcing plate of an auxiliary calibration strip for nucleic acid mass spectrometry data reading proposed in this utility model;

[0019] Figure 3This is a partial structural diagram of the protective film of an auxiliary calibration strip for nucleic acid mass spectrometry data reading proposed in this utility model;

[0020] Figure 4 This is a partial structural diagram of the substrate body of an auxiliary calibration strip for reading nucleic acid mass spectrometry data proposed in this utility model.

[0021] Legend:

[0022] 1. First molecular weight label; 2. Protective film; 3. Second molecular weight label; 4. Third molecular weight label; 5. Positioning hole; 6. QR code; 7. Indicating arrow; 8. Text label; 9. Substrate body; 10. Reinforcing plate; 11. Magnetic plate; 12. Low molecular weight standard point; 13. Medium molecular weight standard point; 14. High molecular weight standard point. Detailed Implementation

[0023] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0024] Reference Figure 1 - Figure 4 An embodiment of this utility model provides an auxiliary calibration sheet for reading nucleic acid mass spectrometry data, including a reinforcing plate 10, a substrate assembly fixedly connected to the top of the reinforcing plate 10, a protective film 2 fixedly connected to the top of the substrate assembly, and a magnetic suction plate 11 fixedly connected to the bottom of the reinforcing plate 10. Positioning holes 5 are uniformly opened inside the protective film 2, the substrate assembly, the reinforcing plate 10, and the magnetic suction plate 11. A first molecular weight label 1 is fixedly connected to one end of the top side of the protective film 2, a second molecular weight label 3 is fixedly connected to the middle of the top side of the protective film 2, a third molecular weight label 4 is fixedly connected to the other end of the top side of the protective film 2, and a QR code 6 is provided on the other end of the top side of the protective film 2.

[0025] Specifically, the reinforcing plate 10 provides structural support for the calibration strip, preventing bending and deformation during use and ensuring calibration accuracy; the substrate assembly is the core of the calibration, carrying standard substances of different molecular weights and providing a calibration benchmark for nucleic acid mass spectrometry detection; the protective film 2 covers the top of the substrate assembly, preventing contamination or wear of the standard substances, while the transparent material does not affect the reading of the mass spectrometry signal; the magnetic plate 11 can be adsorbed onto the magnetic stage of the mass spectrometer, realizing rapid positioning and fixation of the calibration strip and avoiding displacement; the positioning hole 5 penetrates through each layer of the structure and can cooperate with the positioning column of the mass spectrometer stage to further improve the installation accuracy; the first molecular weight label 1, the second molecular weight label 3, and the third molecular weight label 4 respectively mark the molecular weight range of the corresponding area, making it convenient for operators to quickly identify the calibration range; the QR code 6 stores information such as the specifications, batch, and expiration date of the calibration strip, which can be retrieved by scanning, improving the convenience of use.

[0026] Reference Figure 2 - Figure 4 The substrate assembly includes a substrate body 9. Low molecular weight standard points 12 are uniformly arranged on one side of the top of the substrate body 9. Medium molecular weight standard points 13 are uniformly arranged in the middle of one side of the top of the substrate body 9. High molecular weight standard points 14 are uniformly arranged on the other side of the top of the substrate body 9. The top of the substrate body 9 is fixedly connected to the bottom of the protective film 2. Positioning holes 5 are uniformly opened inside the substrate body 9.

[0027] Specifically, the substrate body 9 serves as the foundation for the standard points, and its material is stable and does not interfere with the mass spectrometry detection signal. The low molecular weight standard points 12, medium molecular weight standard points 13, and high molecular weight standard points 14 correspond to different molecular weight ranges, covering the common detection range of nucleic acid mass spectrometry, and can perform full-range calibration of the signal intensity and molecular weight accuracy of the detection instrument. The uniform distribution of standard points ensures the representativeness of the calibration data. The cooperation between the positioning hole 5 and the substrate body 9 ensures that the substrate assembly is precisely aligned with other layer structures during installation, avoiding the impact of interlayer offset on the correspondence between the standard points and the mass spectrometry detection area.

[0028] Reference Figure 1 - Figure 3 A text label 8 is fixedly connected to the middle of the other side of the protective film 2, and an indicator arrow 7 is fixedly connected to the other end of the other side of the protective film 2. The protective film 2 is made of transparent polyethylene terephthalate film with a thickness of 50-100μm, and the reinforcing plate 10 is made of lightweight aluminum alloy with a thickness of 1-2mm.

[0029] Specifically, the text label 8 on the protective film 2 indicates the instructions for use of the calibration strip, making it convenient for operators to refer to; the indicator arrow 7 clearly indicates the installation direction of the calibration strip to avoid calibration errors caused by reverse installation; the transparent polyethylene terephthalate film material has good chemical stability, light transmittance and wear resistance, and its thickness of 50-100μm can effectively protect the standard points without significantly attenuating the mass spectrometry signal; the reinforcing plate 10 made of lightweight aluminum alloy balances structural strength and lightweight in a thickness of 1-2mm, avoiding the impact of excessive weight on the stability of the mass spectrometer stage, while the aluminum alloy material does not interfere with magnetic adsorption and mass spectrometry detection.

[0030] Working principle: When using the auxiliary calibration strip for nucleic acid mass spectrometry data reading, first confirm the installation direction by using the indicator arrow 7 on the protective film 2. Then, attach the calibration strip to the magnetic stage of the nucleic acid mass spectrometer using the bottom magnetic suction plate 11, ensuring that the positioning holes 5 of each layer align with the positioning posts on the stage for precise positioning. Scan the QR code 6 on the protective film 2 to retrieve the specification parameters and standard point information of the calibration strip as a calibration reference. After starting the mass spectrometer, the instrument's detection beam passes through the transparent protective film 2 sequentially, illuminating the low molecular weight standard point 12 and the medium molecular weight standard point 1 on the substrate body 9. 3. High molecular weight standard point 14: The standard substance in the standard point generates a characteristic mass spectrometry signal; the instrument collects the signal data of each standard point and compares it with the preset standard signal. If there is a deviation, the detection parameters are automatically adjusted until the detection data is consistent with the standard value, thus completing the instrument calibration; during the calibration process, the protective film 2 prevents the standard points from being contaminated or worn, and the reinforcing plate 10 ensures that the calibration sheet remains flat, avoiding the detection area shift due to deformation; after calibration, the calibration sheet can be removed for nucleic acid sample detection. With the assistance of the calibration sheet, the accuracy and repeatability of nucleic acid mass spectrometry data reading are improved.

[0031] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An auxiliary calibration strip for nucleic acid mass spectrometry data reading, comprising an reinforcing plate (10), characterized in that: A substrate assembly is fixedly connected to the top of the reinforcing plate (10), a protective film (2) is fixedly connected to the top of the substrate assembly, and a magnetic suction plate (11) is fixedly connected to the bottom of the reinforcing plate (10). Positioning holes (5) are uniformly opened inside the protective film (2), the substrate assembly, the reinforcing plate (10) and the magnetic suction plate (11). A first molecular weight label (1) is fixedly connected to one end of the top side of the protective film (2), a second molecular weight label (3) is fixedly connected to the middle of the top side of the protective film (2), a third molecular weight label (4) is fixedly connected to the other end of the top side of the protective film (2), and a QR code (6) is provided on one end of the top side of the protective film (2).

2. The auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: The substrate assembly includes a substrate body (9), with low molecular weight standard points (12) uniformly disposed on one end of the top side of the substrate body (9), and medium molecular weight standard points (13) uniformly disposed on the middle of the top side of the substrate body (9).

3. The auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 2, characterized in that: High molecular weight standard points (14) are uniformly arranged on one side of the top of the substrate body (9), and the top of the substrate body (9) is fixedly connected to the bottom of the protective film (2).

4. The auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: The positioning holes (5) are evenly distributed inside the substrate body (9).

5. An auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: A text label (8) is fixedly connected to the middle of the other side of the protective film (2).

6. An auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: An indicator arrow (7) is fixedly connected to the other end of the other side of the protective film (2).

7. An auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: The protective film (2) is made of transparent polyethylene terephthalate film with a thickness of 50-100 μm.

8. An auxiliary calibration strip for nucleic acid mass spectrometry data reading according to claim 1, characterized in that: The reinforcing plate (10) is made of lightweight aluminum alloy with a thickness of 1-2 mm.

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