Fragrance compositions

Abstract

Various embodiments disclosed relate to a fragrance composition. The fragrance composition includes a fragrance component and an extract solution. The extract solution includes nymphaea coerulea flower extract and nelumbo nucifera flower extract.

Description

FRAGRANCE COMPOSITIONS CROSS-REFERENCE TO RELATED APPLICATION [1] This application claims the benefit of priority to U.S. Provisional Patent Application Serial Nos. 63 / 500,388 filed May 5, 2023 entitled “Cosmetic Composition”; 63 / 500,382 filed May 5, 2023 entitled “Fragrance Compositions”; 63 / 614,226 filed December 22, 2023 entitled “Cosmetic Composition”; and NL 2035897 filed 27 Sep 2023 entitled “Fragrance Compositions” the disclosure of which is incorporated herein in its entirety by reference. BACKGROUND Experimental and clinical studies have shown that fragrance oils can display varying evaporation profiles depending at least on the presence or absence of other components of the formula.. Fragrance oils can also act as prehaptens or prohaptens by forming allergens that are more potent than a parent substance by activation outside or in the skin. Activation occurs via abiotic, chemical and physical factors, or biotic activation, thus, increasing the risk of sensitization. Some fragrance oils autoxidize on contact with air, forming potent sensitizers that can be an important source for contact allergy to fragrances and fragranced products. Some of them act as prohaptens and are activated in the skin as well. SUMMARY OF THE INVENTION [2] Various embodiments disclosed relate to a fragrance composition. The fragrance composition includes a fragrance component; optionally a modulator; and an extract solution capable of sebum reduction; sweat reduction; and / or acetylcholine release inhibition. The extract solution may include nymphaea coerulea flower extract and nelumbo nucifera flower extract. The synergistic effects of applying the modulator and extract solution prior to or simultaneously with application of a fragrance allergen to skin may result in decreased allergenicity. [3] New ingredients for use as effective, active agents in compositions and formulations for application, preferably topical application, to the skin are advantageous to the industry and the consumer for a variety of uses in the areas of skin care treatment and therapy, and personal use products. Nelupure (INCI: Propanediol (and) Glycerin (and) NymphaeaCaerulea Flower Extract (and) Nelumbo Nucifera Flower Extract, used interchangeably as trade name Nelupure; INCI; “lotus flower extract”; and / or “lotus flower extract blend and / or constituents thereof” throughout) is a blended extract from two types of lotus flower, the Egyptian Lotus (Nymphaea caerulea) and the Sacred Lotus (Nelumbo nucifera). It has been speculated that Nelupure (INCI: Propanediol (and) Glycerin (and) Nymphaea Caerulea Flower Extract (and) Nelumbo Nucifera Flower Extract) acts via leptin-receptor inhibition to regulate the sebaceous glands, preventing the excess of sebum production and inflammatory processes that cause skin blemishes, and may also act to inhibit acetylcholine release. [4] Unwind Sacred Lotus, marketed by the Naolys company, is a material consisting of active plant cells from Nelumbo nucifera whose action has been described as " modulating the skin’s neuro-system, Sacred Lotus cells have a relaxing effect that helps to restore the skin’s original balance.” https: / / www.unifect.com / products / unwind-sacred-lotus / BRIEF DESCRIPTION OF THE FIGURES [5] The drawings illustrate generally, by way of example, but not by way of limitation, various embodiments discussed in the present document. [6] Figure 1 illustrates a summary of steps of the GARDskin dose response method. [7] Figure 2 graphically illustrates application of the GARDskin dose response protocol in a titrated range of concentrations (n=6) starting from a determined top concentration. DETAILED DESCRIPTION OF THE INVENTION [8] Reference will now be made in detail to certain aspects of the disclosed subject matter. While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the exemplified subject matter is not intended to limit the claims to the disclosed subject matter. [9] Throughout this document, values expressed in a range format should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. For example, a range of “about 0.1% to about 5%” or “about 0.1% to 5%” should be interpreted to include not just about 0.1% to about 5%, but also the individual values (e.g., 1%, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within theindicated range. The statement “about X to Y” has the same meaning as “about X to about Y,” unless indicated otherwise. Likewise, the statement “about X, Y, or about Z” has the same meaning as “about X, about Y, or about Z,” unless indicated otherwise.

[0010] In this document, the terms “a,” “an,” or “the” are used to include one or more than one unless the context clearly dictates otherwise. The term “or” is used to refer to a nonexclusive “or” unless otherwise indicated. The statement “at least one of A and B” has the same meaning as “A, B, or A and B.” In addition, it is to be understood that the phraseology or terminology employed herein, and not otherwise defined, is for the purpose of description only and not of limitation. Any use of section headings is intended to aid reading of the document and is not to be interpreted as limiting; information that is relevant to a section heading may occur within or outside of that particular section.

[0011] As used herein, the term “body splash” means a body care formulation that is applied to the body. Typically, the body splash is applied to the body after bathing and provides a subtle hint of scent to the body. Body splashes are commonly used by consumers who prefer less strong fragrance compositions. A body splash may comprise an ethanol-free composition according to the present invention which comprises from 0.2-8 wt%, relative to the total weight of the composition, of a fragrance component. The body splash may further comprise alkyl polyglucosides as non-ionic surfactants.

[0012] As used herein, the term “body spray” means a formulation comprising fragrance materials intended to be applied to the body to prevent or mask body odor caused by the bacterial breakdown of perspiration on the body (e.g., armpits, feet, and other areas of the body). The body spray may also provide a fragrance expression to the a panel of experts or professional evaluators or individual experts or professional evaluators. Typically, body spray compositions are applied as an aerosol spray in an effective amount on the skin of a consumer.

[0013] As used herein, the term “composition” includes a fine fragrance composition intended for application to a body surface, such as for example, skin or hair, e.g., to impart a pleasant odor thereto, or cover a malodour thereof. They are generally in the form of perfume concentrates, perfumes, eau de parfums, eau de toilettes, aftershaves, or colognes. The fine fragrance compositions may be an ethanol-based composition. The term “composition” may also include a cosmetic composition, which comprises a fragrance material for the purposes of delivering a pleasant smell to drive consumer acceptance of the cosmetic composition. The term “composition” may also include body splashes or body sprays. The term “composition” may also include cleaning compositions, such as fabric care composition or home carecompositions, including air care compositions (e.g., air fresheners), for use on clothing or other substrates such as hard surfaces (e.g., dishes, floors, countertops). Additional non-limiting examples of “composition” may also include facial or body powder, deodorant, foundation, body / facial oil, mousse, creams (e.g., cold creams), waxes, sunscreens and blocks, bath and shower gels, lip balms, self-tanning compositions, masks and patches.

[0014] As used herein, the term “consumer” means both the user of the composition and the observer nearby or around the user.

[0015] As used herein, the term “fragrance material” and “fragrance materials” relates to a perfume raw material (“PRM”), or a mixture of perfume raw materials (“PRMs”), that are used to impart an overall pleasant odor or fragrance profile to a composition. “Fragrance materials” can encompass any suitable perfume raw materials for fragrance uses, including materials such as, for example, alcohols, aldehydes, ketones, esters, ethers, acetates, nitriles, terpene hydrocarbons, nitrogenous or sulfurous heterocyclic compounds and essential oils. However, naturally occurring plant and animal oils and exudates comprising complex mixtures of various chemical components are also known for use as “fragrance materials”. The individual perfume raw materials which comprise a known natural oil can be found by reference to Journals commonly used by those skilled in the art such as “Perfume and Flavourist” or “Journal of Essential Oil Research”, or listed in reference texts such as the book by S. Arctander, Perfume and Flavor Chemicals, 1969, Montclair, New Jersey, USA and more recently re-published by Allured Publishing Corporation Illinois (1994). Additionally, some perfume raw materials are supplied by the fragrance houses (Firmenich, International Flavors & Fragrances, Givaudan, Symrise) as mixtures in the form of proprietary specialty accords. Non-limiting examples of the fragrance materials useful herein include pro-fragrances such as acetal pro-fragrances, ketal pro-fragrances, ester pro-fragrances, hydrolyzable inorganic- organic pro-fragrances, and mixtures thereof. The fragrance materials may be released from the pro-fragrances in a number of ways. For example, the fragrance may be released as a result of simple hydrolysis, or by a shift in an equilibrium reaction, or by a pH-change, or by enzymatic release.

[0016] As used herein, the term “fragrance profile” means the description of how the fragrance is perceived by the human nose at any moment in time. The fragrance profile may change over time. It is a result of the combination of the low, moderate and high volatile fragrance materials, if present, of a fragrance. A fragrance profile is composed of 2 characteristics: ‘intensity’ and ‘character’. The ‘intensity’ relates to the perceived strengthwhilst ‘character’ refers to the odor impression or quality of the perfume, e.g., fruity, floral, woody, etc.

[0017] As used herein, the terms “modulator”, and “fragrance modulator” are used interchangeably to designate an agent having the capacity to affect the fragrance profile, such as for example, by impacting the fragrance materials’ evaporation rate. The modulator may mediate its effect by lowering the vapor pressure of the fragrance materials and increasing their adherence to the substrate (skin and / or hair) thus ensuring a longer-lasting impression of the fragrance. By incorporating the modulator, it is desired that the fragrance profile, preferably the fragrance components composition attributable to the high and moderate volatile fragrance materials, alone or individually, of the composition can be perceived by a panel of experts or professional evaluators or individual experts or professional evaluators, over a longer period of time, or the perceived harshness of overdosing of the fragrance material is mitigated or absent, as compared to the same perception in the absence of the modulator. As used herein “overdose” can include overdosing a moderate volatile component or high volatile component in aggregate (e.g., greater than 30 wt% of the fragrance component). The term “overdose” can further include overdosing an individual component of the moderate volatile component or the high volatile component (e.g., if the high volatile component includes three oils at least one of the oils may account for a greater wt% of the high volatile component than would be present in a traditional fragrance or a fragrance that is free of the modulators described herein). Suitable examples of the modulator are provided herein below.

[0018] As used herein, “skin sensitizing potency” is definedrelative ability of a chemical to induce skin sensitization, which is determined by the amount of chemical per unit area required for the acquisition of skin sensitization in a previously naïve individual. For chemical safety assessment, it is of utmost importance to evaluate dose-effect relationships and to classify chemicals according to the strength of their response. Skin sensitizing potency is measured by a GARDskin dose response method. Figure 1 illustrates a summary of steps of the GARDskin dose response method. Figure 2 graphically illustrates application of the GARDskin dose response protocol in a titrated range of concentrations (n=6) starting from a determined top concentration.

[0019] As used herein, the term “substantially non-odorous” means an agent that does not impart an odor of its own when added into a composition of the present invention. For example, a “substantially non-odorous fragrance modulator” does not impart a new odor thatalters the character of the fragrance profile of the composition to which it is added. The term “substantially non-odorous” also encompasses an agent that may impart a minimal or slight odor of its own when added into a composition of the present invention. However, the odor imparted by the “substantially non-odorous fragrance modulator” is generally undetectable or tends to not substantively alter the character of the fragrance profile of the composition to which it is added initially or preferably over time. Furthermore, the term “substantially non- odorous” also includes materials that are perceivable only by a minority of people or those materials deemed “anosmic” to the majority of people. Furthermore, the term “substantially non-odorous” also includes materials that may, from particular suppliers, contain an odor due to impurities, such as when the materials contain the impurities at not more than about 5 wt%, preferably not more than 1 wt%, often even not more than 1 part per million (ppm). These impurities may be removed by purification techniques known in the art as required to make them suitable for use in fragrance compositions of the present invention.

[0020] As used herein, the term “vapor pressure” means the partial pressure in air at a defined temperature (e.g., 25 °C) and standard atmospheric pressure (760 mmHg) for a given chemical species. It defines a chemical species’ desire to be in the gas phase rather than the liquid or solid state. The higher the vapor pressure the greater the proportion of the material that will, at equilibrium, be found in a closed headspace. It is also related to the rate of evaporation of a fragrance material which is defined in an open environment where material is leaving the system. The vapor pressure is determined according to the reference program Advanced Chemistry Development (ACD / Labs) Software Version 14.02, or preferably the latest version update).

[0021] It is understood that the test methods that are disclosed in the Test Methods Section of the present application must be used to determine the respective values of the parameters of Applicants’ inventions as described and claimed herein.

[0022] In all embodiments of the present invention, all percentages are by weight of the total composition, as evident by the context, unless specifically stated otherwise. All ratios are weight ratios, unless specifically stated otherwise, and all measurements are made at 25 °C, unless otherwise designated.

[0023] According to various aspects of the instant disclosure, a fragrance composition includes a fragrance component as well as an extract solution capable of reducing sebum production; sweat production; and / or acetylcholine release. The extract solution includes nymphaea coerulea flower extract and nelumbo nucifera flower extract. The solution isavailable as a product under the tradename NELUPURE (INCI: INCI: Propanediol (and) Glycerin (and) Nymphaea Caerulea Flower Extract (and) Nelumbo Nucifera Flower Extract). The extract solution can be present in a range of from about 0.5 wt% to about 20 wt% of the fragrance composition, about 2 wt% to about 10 wt%, less than, equal to, or greater than, 0.5 wt%, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, or about 20 wt%.

[0024] More specifically, the components (also referred to as active agents, constituents, ingredients, reagents, substances, or compounds herein) of this invention are extracts from the flower of one or more species of the lotus flowers, obtained from plants and the like, preferably of the Nelumbo family. These plant materials have been newly determined to be effective constituents in compositions and methods for treating, preventing, reducing, inhibiting, improving and / or eliminating wrinkles, fine lines, creases, folds, furrows, and other signs of dermatological aging due to chronological and / or hormonal aging, or due to sunlight exposure of the skin, as well for use in the treatment of hyperhidrosis; and may be useful (due to acetylcholine release inhibition and / or decreased sebum or sweat production leading to decreased inflammation) in lessening allergenic reaction to a fragrance composition applied to skin simultaneously with or subsequent to application of the lotus flower extract; and / or in modulating fragrance component evaporation when applied prior to or simultaneously with application of fragrance formulation components to the skin.

[0025] According to the present invention, yet without wishing to be bound by theory, the extracts from the flower of one or more species of the lotus flower constituents exert their effects by their ability to inhibit, block, reduce, or prevent the release of acetylcholine at the neuromuscular junction (NMJ) of skeletal muscle cells and tissue, thereby relaxing muscles, for example, muscles associated with facial movement or expressions. Both nerve and muscle cells (skeletal muscle cells) are electrically excitable and the junctions between these types of cells are known as NMJ. It has been known for many years that the stimulation of a motor nerve innervating a skeletal muscle also causes the release of acetylcholine and that acetylcholine, in turn, stimulates the skeletal muscle to contract. Thus, acetylcholine has been identified as the neurotransmitter at the neuromuscular junction. (see, e.g., B. Alberts et al., 1989, Molecular Biology of the Cell, 2ndEd., Chapter 19, The Nervous System, Garland Publishing, Inc., NY., pp. 1075 et seq.). Provided by the present invention are extracts from the flower of one or more species of the lotus flower constituents which are newly described herein to inhibit, reduce, block, or prevent the release of acetylcholine at the NMJ of the skeletalmuscle and thus relax the muscles, preferably those associated with facial expression or movement, particularly when employed in the compositions and methods of the invention. By being able to inhibit acetylcholine release, compositions and methods comprising the extracts from the flower of one or more species of the lotus flower constituents as described herein can reduce, treat, and / or prevent excessive sweating or perspiration.

[0026] The ability of the extracts from the flower of one or more species of the lotus flower constituents to inhibit acetylcholine release by muscle cells results in a modulation of motor contraction so as to relax the muscle fibers.

[0027] Thus, in one of its embodiments, the present invention encompasses compositions, formulations and methods containing components, preferably, extracts from the flower of one or more species of the lotus flower constituents, newly determined to be useful in the minimization of allergy elicitation associated with fragrance ingredient exposure and / or the inflammatory process.

[0028] The plant-derived materials from the Lotus family may exert their effectiveness according to this invention by preferably working at the neuromuscular junctions (NMJ), such as in and around muscle and nerve cells and tissue at a site of application, e.g., the skin of face, neck, arms, feet, hands, or chest, or in the dermis layer of the skin where sweat glands are located. Prior to the present invention, these materials in combination were not previously known or recognized to inhibit acetylcholine release at NMJ of skeletal muscles.

[0029] In general, for the purposes of the present invention, a substance, such as a extract from the flower of one or more species of the lotus flower constituent of the described compositions, is recognized as being a muscle relaxant when it elicits a relaxation effect on contracted muscle cells or on tissue, such as cutaneous or subcutaneous muscle tissue, and / or exhibits an inhibitory effect on acetylcholine release at the NMJ. Contracted muscle cells or tissue is associated with formation of wrinkles, fine lines, etc.

[0030] It is another aspect of the present disclosure to provide compositions, formulations and methods utilizing materials that are newly determined to be useful in the reduction of sweating from the skin.

[0031] The physiological act of sweating serves as the body's natural coolant, protecting it from overheating. When subjected to temperature increases, stress, or exercise, the body excretes sweat, a fluid comprising mostly water along with minerals, lactate and urea, to cool the body by evaporation of the water.

[0032] Sweating can have adverse effects on the use of cosmetics, for example by adversely impacting the ability of foundation to maintain integrity on the skin. While occlusive agents have been utilized to decrease the amount of perspiration, such occlusive agents can also hinder the skin’s ability to breathe and to release oils, which can result in skin irritation and comedogenic effects.

[0033] As used herein, "sweating" refers to the excretion of perspiration from the pores of the skin. Sweating includes, but not limited to, non-pathological sweating, such as thermally- induced, exercise-induced, or stress-induced sweating. The invention is useful in any area of the body where sweating occurs such as the hands, feet, face, head, torso, neck, arms, wrist, legs, or underarms (axilla).

[0034] It is an object of the present invention to provide a cosmetic composition that does not require the presence of an aluminum salt and / or an occlusive agent to deliver a sweat management benefit.

[0035] For use in the compositions of this invention, the extracts from the flower of one or more species of the lotus flower extracts can be chemically synthesized. Alternatively, these plant alkaloids may be extracted from plants. When extracted, they may be in a pure form, a semi-purified form, or may be a component of an unpurified plant extract.

[0036] The extracts from the flower of one or more species of the lotus flower plant materials contained in the compositions of the present invention can be chemically synthesized at industrial scale in large amounts. Alternatively, the materials may be extracted from natural raw materials from plants. Any and all methods of preparation may be used, including the use of cultured plant seed cells, as disclosed in commonly owned patent application U.S. Ser. No. 10 / 040,242, which is hereby incorporated by reference herein. For example, unpurified plant extracts may be employed in the present invention. Depending on the solubility of the particular plant extractin water or in organic solvent, the extraction process may differ. Two methods for extracting the materials from raw plant materials include organic solvent extraction, and aqueous-organic solvent extraction, as described in U.S. Pat. No. 6,372,239 to Wu et al. The organic extraction method involves a step of continuous washing and extracting the plant material against a stream of organic solvent. Examples of organic solvents include, but are not limited to methanol, ethanol, dichloromethane, chloroform, xylene, and petroleum ether. Alternatively, the alkaloid can be partially purified or completely purified. Chemical synthesis of the alkaloid obviates the need for extraction and purification.

[0037] In accordance with this invention, the extracts from the flower of one or more species of the lotus flower constituents comprise compositions which include, without limitation, topically applied formulations, anti-oxidants, anti-inflammatories, sunscreens, cosmetics, including make-ups, anti-aging formulations, e.g., creams for fine lines and / or wrinkles, topicals, skin penetration enhancers, antiperspirants, deodorants, and the like. Also in accordance with this invention, the extracts from the flower of one or more species of the lotus flower constituents and additional ingredients comprising such compositions can be formulated in a variety of product forms. Preferably, the compositions are prepared in targeted delivery systems, e.g., transdermals, such as patches, and the like, particularly for topical administration.

[0038] The present invention encompasses compositions comprising one or more extracts from the flower of one or more species of the lotus flower constituents, preferably in a pharmaceutically-acceptable cosmetic or dermatological formulation which is suitable for contact with living animal tissue, including human tissue, and for topical administration, with virtually no adverse physiological effect, e.g., irritation, to the user. Thus, the inventive compositions are especially suitable for sensitive skin.

[0039] The fragrance component can include one or more fragrant compounds. On the whole, the fragrance component present in an amount in a range of from about 0.04 wt% to about 40 wt% of the fragrance composition, about 0.5 wt% to about 30 wt%, less than, equal to, or greater than about 0.04 wt%, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, 20, 20.5, 21, 21.5, 22, 22.5, 23, 23.5, 24, 24.5, 25, 25.5, 26, 26.5, 27, 27.5, 28, 28.5, 29, 29.5, 30, 30.5, 31, 31.5, 32, 32.5, 33, 33.5, 34, 34.5, 35, 35.5, 36, 36.5, 37, 37.5, 38, 38.5, 39, 39.5, or about 40 wt%.

[0040] The fragrance component can include a wide variety of fragrance materials. The fragrance materials can be grouped in terms of their volatility. Generally, the materials can be grouped as low volatile fragrance materials, moderate volatile fragrance materials, and high volatile fragrance materials. Each group of materials can be associated with various perceptions by a panel of experts or professional evaluators or individual experts or professional evaluators. While not so limited, a high volatile fragrance may be associated with a citrus character; a moderate volatile fragrance may be associated with a spicy character; and a low volatile fragrance may be associated with a woody character. Each group of fragrance materials caninclude synthetic materials or natural materials. The volatility of the fragrance materials can be in reference to an individual fragrance material. Alternatively, in cases where a combination of materials produce a fragrance, for example a natural oil, the volatility may be in reference to that aggregation.

[0041] As described herein, the “fragrance materials” or “fragrance components” have been classified as low, moderate or high volatile fragrance materials according to their volatility by their vapor pressure. This method of classifying fragrance materials by their vapor pressure avoids the problem of different classifications for the same fragrance material according to the traditional approach that relies on their subjective characteristic character. In the case that the fragrance materials are a natural oil, extract or absolute, which comprises a mixture of several compounds, the vapor pressure of the complete oil should be treated a mixture of the individual perfume raw material components using the reference program cited above. The individual components and their level, in any given natural oil or extract, can be determined by direct injection of the oil into a GC-MS column for analysis as known by one skilled in the art. In the scenario that the fragrance materials are a proprietary specialty accord, so called ‘bases’, the vapor pressure, using the reference program cited above, should preferably be obtained from the supplier. However, it is understood by one skilled in the art that they can physically analyze the composition of a full fragrance oil available commercially to identity the fragrance raw materials and their levels using standard GC-MS techniques. This would be irrespective of whether they had been added to the fragrance oil as individual chemicals, as components of naturals or from proprietary bases. Although proprietary bases and naturals are included in our examples, when analyzing a commercially available fragrance via GC-MS one could simply identify the components of the base or natural oil as part of the overall fragrance mixture and their levels, without being able to identify which proprietary base or natural oil the fragrance had come from.

[0042] A pH of the composition can be in a range of from about 4 to about 8, about 5 to about 7, less than, equal to, or greater than about 4, 5, 6, 7, or about 8. The ethanol concentration in any of the compositions described herein can be less than about 78 wt% ethanol, less than about 70 wt% ethanol, less than about 60 wt% ethanol, less than about 50 wt% ethanol, less than about 40 wt% ethanol, less than about 30 wt% ethanol, less than about 20 wt% ethanol, less than about 10 wt% ethanol, or free of ethanol. (i) Low Volatile Fragrance Materials

[0043] The fragrance component may comprise at least one low volatile fragrance material. Individual low volatile fragrance materials or aggregate low volatile fragrance materials are those having a vapor pressure less than 0.001 Torr (0.000133 kPa) at 25 °C. According to some examples, the composition can include at least 3 low volatile fragrance materials, or at least 4 low volatile fragrance materials, or at least 5 low volatile fragrance materials, or at least 7 low volatile fragrance materials.

[0044] If there are more than one low volatile fragrance materials, then the ranges provided hereinabove cover the total of all the low volatile fragrance materials. Examples of suitable low volatile fragrances materials are provided in Table 1A and 1B below.

[0045] Preferably, the low volatile fragrance material is selected from at least 1 material, or at least 2 materials, or at least 3 materials, or at least 5 materials, at least 7, at least 8, at least 10, or at least 12 low volatile fragrance materials as disclosed in Table 1A. Natural fragrance materials or oils having an aggregrate vapour pressure less than 0.001 Torr (0.000133 kPa) at 25 °C are provided in Table 1B. Low Volatile Natural Oils. Table 1A – Low Volatile Fragrance Materials1 Non-limiting examples of alternative qualities from various suppliers can be purchased under the following tradenames: Kharismal®Super (IFF), Kharismal®(IFF), Hedione®(Firmenich), Hedione®HC (Firmenich), Paradisone (Firmenich), Cepionate (Zenon), Super cepionate (Zenon), Claigeon®(Zenon). * Vapor Pressures are acquired as described in the Test Methods Section. ** Origin: The low volatile fragrance materials may be obtained from one or more of the following companies: Firmenich (Geneva, Switzerland), Symrise AG (Holzminden, Germany), Givaudan (Argenteuil, France), IFF (Hazlet, New Jersey), Bedoukian (Danbury, Connecticut),Sigma Aldrich (St. Louis, Missouri), Millennium Specialty Chemicals (Olympia Fields, Illinois), Polarone International (Jersey City, New Jersey), and Aroma & Flavor Specialties (Danbury, Connecticut). Table 1B -Low Volatile Natural Oils.Suppliers: Firmenich, Geneva, Switzerland Global Essence Inc, New Jersey, USA H. Reynaud & Fils, Montbrun-les-Bains, France IFF, Hazlet, New Jersey, USA Mane, Le Bar-sur-Loup, France Robertet, Grasse, France

[0046] Exemplary low volatile fragrance materials selected from the group of Tables 1A or 1B Low Volatile Fragrance Materials are preferred. However, it is understood by one skilled in the art that other low volatile fragrance materials, not recited in Tables 1A or 1B, would also fall within the scope of the present invention, so long as they have a vapor pressure less than 0.001 Torr (0.000133 kPa) at 25 °C. (ii) Moderate Volatile Fragrance Materials

[0047] The fragrance component may include at least one moderate volatile fragrance material or aggregate of volatile fragrance materials having a vapor pressure in the range of 0.1 Torr to 0.001 Torr (0.0133 kPa to 0.000133 kPa) at 25 °C. In some examples, the composition according to the present disclosure can include at least 3 moderate volatile fragrance materials, or at least 5 moderate volatile fragrance materials, or at least 7 moderate volatile fragrance materials. If there are more than one moderate volatile fragrance materials, then the ranges provided hereinabove cover the total of all of the moderate volatile fragrance materials. Suitable examples of moderate volatile fragrances materials are provided in Table 2A and 2B below.

[0048] Preferable examples of moderate volatile fragrance materials having a vapor pressure in the range of 0.1 Torr to 0.001 Torr (0.0133 kPa to 0.000133 kPa) at 25 °C are provided in Table 2A and 2B. Preferably, the moderate volatile fragrance material is selected from at least 1 material, or at least 2 materials, or at least 3 materials, or at least 5 materials, or at least 7 moderate volatile fragrance materials as disclosed in Table 2A. Natural fragrance materials or oils having an aggregate vapor pressure between 0.1 Torr to 0.001 Torr (0.0133 kPa to 0.000133 kPa) at 25 °C are provided in Table 2B. Moderate Volatile Natural Oils. Table 2A – Moderate Volatile Fragrance Materials* Vapor Pressures are acquired as described in the Test Methods Section. ** Origin: Same as for Table 1 hereinabove. Table 2B--Moderate Volatile Natural Oils.Suppliers: Citrus & Allied Essences, New York, USA H. Reynaud & Fils, Montbrun-les-Bains, France IFF, Hazlet, New Jersey, USA Robertet, Grasse, France

[0049] Moderate volatile fragrance materials can be selected from the group of Tables 2A or 2B. However, it is understood by one skilled in the art that other moderate volatile fragrance materials, not recited in Tables 2A or 2B, would also fall within the scope of the present invention, so long as they have a vapor pressure of 0.1 to 0.001 Torr at 25 °C. (iii) High Volatile Fragrance Materials

[0050] The fragrance component may include at least one high volatile fragrance material having a vapor pressure greater than 0.1 Torr (0.0133 kPa) at 25 °C. In some examples, the high volatile fragrance material can include at least 2 high volatile fragrance materials, 3 high volatile fragrance materials, or at least 5 high volatile fragrance materials, or at least 7 high volatile fragrance materials. If there are more than one high volatile fragrance materials, then the ranges provided hereinabove cover the total of all of the high volatile fragrance materials. Suitable examples of high volatile fragrances materials are provided in Tables 3A and 3B below.

[0051] Preferably, the high volatile fragrance material is selected from at least 1 material, or at least 2 materials, or at least 3 materials, or at least 5 materials, at least 7 materials, or at least 9 high volatile fragrance materials as disclosed in Table 3A. Natural fragrance materials or oils having an aggregate vapor pressure greater than 0.1 Torr (0.0133 kPa) at 25 °C are provided in Table 3B. Moderate Volatile Natural Oils. Table 3A – High Volatile Fragrance Materials* Vapor Pressures are acquired as described in the Test Methods Section. ** Origin: Same as for Table 1 hereinabove. Table 3B— High Volatile Natural MaterialsSuppliers Biolandes, Le Sen, France Capua, Campo Calabro, Italy Citrus & Allied Essences, New York, USA Firmenich, Geneva, Switzerland Global Essence Inc, New Jersey, USA H. Reynaud & Fils, Montbrun-les-Bains, France IFF, Hazlet, New Jersey, USAKerry, Co. Kerry, Ireland Mane, Le Bar-sur-Loup, France Misitano & Stracuzzi, Messina, Italy Robertet, Grasse, France Simone Gatto, San Pierre Niceto, Italy

[0052] Exemplary high volatile fragrance materials selected from the group of Tables 3A or 3B are preferred. However, it is understood by one skilled in the art that other high volatile fragrance materials, not recited in Tables 3A or 3B, would also fall within the scope of the present invention, so long as they have a vapor pressure of greater than 0.1 Torr (0.0133 kPa) at 25 °C.

[0053] The individual fragrance materials can be present in various concentrations of the fragrance component. For example in a “diamond construction” the low volatile material can be present in a range of from about 0 wt% to about 30 wt% of the fragrance component, about 10 wt% to about 20 wt%, less than equal to or greater than about 0 wt%, 5, 10, 15, 20, 25, or 30 wt%; the moderate volatile component can be present in a range of from about 30 wt% to about 70 wt% of the fragrance component, about 40 wt% to about 60 wt%, less than, equal to, or greater than about 30 wt%, 35, 40, 45, 50, 55, 60, 65, or about 70 wt%; the high volatile fragrance component can be present in a 0 wt% to about 30 wt% of the fragrance component, about 10 wt% to about 20 wt%, less than equal to or greater than about 0 wt%, 5, 10, 15, 20, 25, or 30 wt%.

[0054] The composition can further include at least one modulator as described herein below. Suitable examples of the fragrance modulators include: the compound according to Formula I:a mixture thereof. R1, R2, R3, R4, and R5, are independently chosen from -H, -OH, or substituted or unsubstituted (C1-C20)hydrocarbyl. In some further embodiments, R1, R2, R3, R4, and R5, are independently chosen from (C1-C20)alkyl, (C2-C20)alkeneyl, (C3-C20)cycloalkyl, (C1-C20)alkoxyl, (C1-C20)aryl, or a combination thereof. In some specific embodiments, the modulator component includes pentylene glycol, polycitronellol, or a mixture thereof. In some embodiments, the polycitronellol can include 2-8 repeating units and can have a weight- average-molecular weight in a range of from about 460 g / mol to about 1500 g / mol. In some embodiments, the modulator component can include a mixture of pentylene glycol and polycitronellol and a molar ratio of pentylene glycol to polycitronellol is in a range of from about 5:1 to about 1:5, about 4:1 to about 1:4, about 3:1 to about 1:3, or about 2:1 to about 1:2, or about 1:1. In some embodiments, the modulator component can include at least some PPG- 20 methyl glucose ether mixed with any of the aforementioned modulators. Alternativly, the modulator component can be free of (include 0 wt%) PPG-20 methyl glucose. If PPG-20 methyl glucose is present, it can be in range of from about 1 wt% to about 15 wt% of the modulator component, about 5 wt% to about 10 wt% of the modulator component, less than, equal to, or greater than about 1 wt%, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or about 15 wt%.

[0055] In some further examples, the modulator component can include other modulators included in addition to pentylene glycol, polycitronellol, or a mixture thereof. Examples of the additional modulators can include those listed herein below in Tables 4(a) and 4(b). Tables 4(a) and 4(b) provide lists of suitable non-odorous fragrance modulators. Table 4(a): Substantially Non-Odorous Fragrance Modulators1available as GLUCAM™ P-20.2available as Glucam™ E-20.3available as Plantacare®810 UP.3aavailable as Simulsol®SL 11W.4available as CERAPHYL®ICA.5available as Tegosoft®APM.6available as Schercemol™ NGDO. 7 disclosed in U.S. Patent No.6,737,396B2 (Firmenich), column 1, lines 43-47. 8 diclosed as compound 1’i in U.S. Patent No.6,440,400B1 (Takasago Int. Corp.), col.5. 8adiclosed in U.S. Patent No.4,313,855 (Dragoco Gerberding & Co. GmbH), col.1, lines 12- 13. 9 disclosed in U.S. Patent No.7,538,081B2 (Takasago Int. Corp.), column 7, lines 50-53. 10disclosed in U.S. Patent No.6,147,049 (Givaudan Roure), col.5, line 24, to col.6, line 17. 11disclosed in PCT Publication No. WO85 / 04803 (Diagnostic), pg.2, line 1 to pg.4, line 2. 12disclosed in JP Patent No.61-083114 (Kanebo). 13disclosed in JP Patent No.61-063612 (Kanebo). 14disclosed in JP Patent No.62-084010 (Shiseido). 14bavailable as: Laureth-6. 15disclosed in U.S. Patent Publication No.2011 / 0104089A1 (Symrise), para.

[0001] . 16available as PCL-Liquid®100. 17disclosed in U.S. Patent No.7,196,052 (Takasago Int. Corp.), col.4, lines 34-35. 18disclosed in EP Patent Publication No.616800A2 (Givaudan), pg.2, lines 12-25. 19disclosed in U.S. Patent No.4,110,626 (Shiseido), column 3, lines 54-56. 19adisclosed in PCT Publication No. WO2014 / 155019 (LVMH). 19bdisclosed in U.S. Patent No.9,050,261 (Symrise). 20disclosed as compounds C1-C22 in WO2014 / 139952 (Unilever). 21available as Expert Gel®EG56. 22available as Kolliphor®EL. 23disclosed in U.S. Patent No.9,050,261 (Symrise). Further examples of non-odorous fragrance modulator is selected from the group of materials disclosed in Table 4(b). Table 4(b): Substantially Non-Odorous Fragrance Modulators

[0056] According to some examples, the modulator(s) can be characterized as being “low odor”, “substantially non-odorous”, or non-odorous. In some examples, if the modulator is present at 1 wt% or less, no odor may be detected from the modulator.

[0057] In some examples, the fragrance modulator is biodegradable. This can make the fragrance composition to which it is included a “green” or environmentally friendly fragrance composition. Additionally, the stability of a fragrance composition that includes the substantially non-odorus fragrance modulator can be increased. The increase in stability can be shown by observing that the composition undergoes undesirable color formation (e.g., yellowing) at a slower rate.

[0058] The fragrance modulator component can be present in an amount of from about 0.1 wt% to about 27 wt% relative to the total weight of the composition of the composition, about 0.5 wt% to about 18 wt%, about 2.5 wt% to about 15 wt%, or less than, equal to, or greater than about 0.1 wt%, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5, 20 wt%. If there are more than one fragrance modulators, then the ranges provided hereinabove cover the total of all of the fragrance modulators.

[0059] The fragrance modulator can be a liquid at temperatures lower than 100 °C, such as at ambient temperature. The fragrance modulators may be fully miscible with the fragrance materials to form a single phase liquid. However, if the fragrance materials are not entirely miscible, or are immiscible, then co-solvents (e.g., dipropylene glycol (DPG), triethyl citrate, or others well known to those skilled in the art) can be added to aid in the solubility of the fragrance materials.

[0060] According to various examples, the effect of the fragrance modulator on the fragrance profile, particularly the characters of the fragrance profile which is attributable to the high and moderate volatile fragrance materials, can be improved. By “improved” it is meant that the fragrance profile of the composition, particular the components contributed by at leastone of the high and moderate volatile fragrance materials, can be perceived by a panel of experts or professional evaluators or individual experts or professional evaluators at later time points such as, for example, 15 mins, 30 mins, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 10 hours, and possibly all the way up to 30 hrs after application as compared to controls, e.g., lacking any of the disclosed non-odorous fragrance modulators such as pentylene glycol, polycitronellol or an equivalent traditional fragrance construction.

[0061] Alternatively, by “improved” it can mean that the perception, by a panel of experts or professional evaluators or individual experts or professional evaluators, of the fidelity of the fragrance profile contributed by the high and moderate volatile fragrance materials is markedly increased or enhanced as compared to the controls. “Increased” or “enhanced” means that a panel of experts or professional evaluators or individual experts or professional evaluators perceives the fragrance profile, preferably the characters attributable to the high and / or moderate volatile fragrance materials, of a composition as not changing from its initial impression or the changes are minimal from when the composition was first applied to when it dissipates. In other words, the fidelity of the perceived fragrance profile of the composition is maintained over time. In contrast the composition lacking any of the disclosed non-odorous fragrance modulators or an equivalent traditional fragrance construction will undergo a rapid loss of the characters attributable to the high and / or moderate volatile fragrance materials.

[0062] Such a solution as presented herein provides enhanced or improved fidelity and / or longevity of the fragrance profile, particularly amongst those composition formulated from volatile fragrance materials having moderate to high vapor pressure ranges (greater than or equal to 0.001 Torr (0.000133 kPa) at 25 °C), without having to rely on the presence or significant amounts of the low volatile fragrance materials, which has a tendency to overpower and alter the overall fragrance profile, particularly over time. As a result, the present disclosure provides the perfumer options to formulate compositions having new fragrance profiles not possible before.

[0063] Specific modulators that can be useful include pentylene glycol, polycitronellol, PPG-20 methyl glucose ether, methyl glucoside polyol, ethyl glucoside polyol, propyl glucoside polyol, or a mixture thereof.

[0064] In some aspects, the fragrance composition can include a carrier. The carrier can be an aqueous carrier, organic carrier, or a mixture thereof. The organic carrier can be ethanol, dipropylene glycol, benzyl benzoate, diethyl phthalate, isopropyl myristate, (C1-C20)alkyl, (C1-C20)alkenyl, (C1-C20)alkynyl, (C1-C20)hydroxyl, or mixtures thereof. Additionally, the carrier can be any of, solutions, aerosols, emulsions (including oil-in-water or water-in-oil), gels, and liposomes.

[0065] The fragrance composition can include an active component. The active component is in a range of from about0.0001 wt% to about 35 wt% of the cosmetic formula, about 20 wt% to about 30 wt%, less than, equal to, or greater than 15 wt%, 16, 171, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or about 35 wt%. The active component can include any component or mixture of components to achieve a desired result. Examples of active components include lactic acid, a hyaluronic acid, vitamin E, saccharomyces / xylinum / black tea ferment, diglucosyl gallic acid, adenosine, panthenol, dimethylmethoxy chromanol, alpha-glucan oligosaccharide, thermus thermophilus ferment, hydrolyzed hyaluronic acid, caffeine, acetyl di-peptide-1 cetyl ester, glycerin, niacinamide, swertia chirata extract, creatine, or a mixture thereof. The mixture of gels described and surfactant(s) herein above, allow for the relatively high amount of active component. The ability of the formulation to have a high amount of active component can enhance the formulation’s effectiveness.

[0066] In some examples, the formulation can include a chelant or a mixture of two or more chelants. Examples of suitable chelants can include tetrasodium glutamate diacetate (GLDA), ethylenediaminetetraacetic acid (EDTA), ethylenediamine-N, N’-disuccinic acid (EDDS), NTA, EDDS, IDS, methylglycinediacetic acid (MGDA), L-glutamic acid N,N- diacetic acid (GLDA), ethylenediamine-N,N-diglutaric acid (EDDG), ethylenediamine-N,N’- dimalonic acid (EDDM), 3-hydroxy-2,2-iminodisuccinic acid (HIDS), 2- hydroxyethyliminodiacetic acid (HEIDA), methylglycine N,N-diacetic acid trisodium salt (MGDA), sodium gluconate, or a mixture thereof.

[0067] The formulas herein can contain a variety of other optional components suitable for rendering such compositions more cosmetically or aesthetically acceptable or to provide them with additional usage benefits. Such conventional optional ingredients are well-known to those skilled in the art. These include any cosmetically acceptable ingredients such as those found in the CTFA International Cosmetic Ingredient Dictionary and Handbook, 7th edition, edited by Wenninger and McEwen, (The Cosmetic, Toiletry, and Fragrance Association, Inc., Washington, D.C., 1997). As used herein “cosmetically acceptable” means a material (e.g., compound or composition) which is suitable for use in contact with skin, hair or other suitable substrate.The fragrance composition can include a cyclodextrin, a film former or a mixture thereof. According to some examples, the film former comprises anionic compounds or polymers, non- ionic compounds or polymers, amphoteric compounds or polymers, zwitterionic compounds or polymers, cationic compounds or polymers, proteins, viscosity modifiers, polyacrylates, polymethacrylates, polyacrylate copolymers, polymethacrylate copolymers, polyamides, polyaminoamides, polyesters, polysaccharides, polyacrylamides, starches, gums, or a mixture thereof. The cyclodextrin can include, but is not limited to, α-cyclodextrin, β-cyclodextrin, gamma-cyclodextrin, methyl-α-cyclodextrin, methyl-β-cyclodextrin, hydroxypropyl-α- cyclodextrin hydroxypropyl-β-cyclodextrin, and mixtures thereof . Most preferred examples of cyclic oligosaccharides for use herein are methyl-α-cyclodextrin and methyl-β-cyclodextrin. These are available from Wacker-Chemie GmbH Hanns-Seidel-Platz 4, Munchen, DE under the tradename Alpha W6 M and Beta W7 M respectively.

[0068] There are many benefits associated with various fragrance compositions associated with the instant disclosure. For example, it is suspected that the fragrance component is detectable for an amount of time longer than a corresponding fragrance composition that free of the extract solution. That is the fragrance materials can be perceived for a long amount of time and thus a user may not have to constantly apply the fragrance composition.

[0069] Additionally, according to various aspects, it is suspected that a user will perceive that the fragrance composition is more comfortable than a corresponding fragrance composition that free of the extract solution. For example, a user may perceive the fragrance composition as not feeling too oily, greasy, or dry.

[0070] Additionally, according to various aspects, it is suspected that the fragrance composition will be more effective at reducing or eliminating malodor (e.g., of an odor that is unpleasant or offensive to the human nose) than a corresponding composition that is free of the extract solution.

[0071] Additionally, according to various aspects, it is suspected that modulation of the fragrance composition is increased relative to a corresponding composition that is free of the extract solution and comprises a cyclodextrin, PPG 20 methyl glucose ether, a chelator, or a mixture thereof. That is the extract can help to increase the intensity of the fragrance materials.

[0072] Additionally, according to various aspects, it is suspected that the fragrance composition can be effective for decreasing skin sensitizing potency of the fragrance component. That is if the fragrance composition includes the extract solution and it is possible that a user’s tendency to be sensitized to the fragrance component can be decreased. Thefragrance composition may also prevent irritation or allergy inducement when applied to an intimate area. According to various aspects, the use of a combination of methyl glucose ether and an extract solution comprising nymphaea coerulea flower extract; and nelumbo nucifera flower extract may decrease fragrance sensitization likelihood and / or severity. Any portion of the aforementioned constitutes can be combined in any desirable manner to produce many different types of cosmetic compositions. The cosmetic compositions can be applied to the epithelial layer of a user’s skin. For example the cosmetic composition can be a pretreatment formulation, make-up formulation, a foundation, a sunscreen, or any other cosmetic applied to a user’s epithelial tissue. Additionally, any portion of the aforementioned constituents can be combined to create a cosmetic formulation that can be applied to a keratinous tissue of a user. For example, the cosmetic formulation can be a mascara, hair dye, nail polish, lip gloss, lipstick or other pigmented / colored cosmetic formulation intended to be a long-wear formulation. Examples

[0073] Various embodiments of the present invention can be better understood by reference to the following Examples which are offered by way of illustration. The present invention is not limited to the Examples given herein.

[0074] EVALUATIONS:

[0075] Evaluations are performed on skin, with a 50uL application of product (one reference and one tested sample). The sample to evaluate and the reference sample are applied on the same wearer. Evaluators are asked to smell the 2 samples randomly and blind. Then they are asked to rate the tested sample versus the reference according to the following scales and following the evaluation protocol:

[0076] Evaluated at 3 different time points: Fresh; 2h 6h (Note: exception of the water- resistant test. Which is evaluated at Fresh and 2h.

[0077] Character evaluation following the “Odor Grading Scale” presented below: 5 - > Perfume unchanged.4 -> Slight perfume character change.3 -> Moderate perfume change but similar character . Not consumer noticeable. 2 -> Large difference in perfume change, consumer noticeable (unacceptable).1 -> Total difference in perfume character (unacceptable)

[0078] Intensity evaluation following “Intensity scale” +2 -> Very Strong (tested sample is much more intense than the reference); +1 -> Strong; 0 -> Same intensity (reference); -1 -> Weaker; -2 -> Very weak (tested sample is much less intense than the reference)

[0079] A minimum of 3 expert panelists and 8 panelists in total are participating in each evaluation, to obtain conclusive results.

[0080] The averages of the character and intensity difference are calculated. The conclusions are based on the following:

[0081] The character result values above 3 are considered as: no character change

[0082] The intensity result values from -0.3 to +0.3 are considered as: no significant difference between the tested sample and the references

[0083] We tested 4 fragrance accords (Accord 1 (fruity), Accord 2 (citrus), Accord 3 (fougere), Accord 4 (floral) in the patent) covering 4 of the biggest olfactive fragrances families. Full fine fragrances were tested as well.

[0084] NELUPURE Formulas and Skin evaluation results:

[0085] Alcoholic chassis (EdT):

[0086] Nelupure is added in the water and then mixed with the ethanol / fragrance oil. Full formula here below:

[0084] Results of the evaluations as per the protocol described above.Perfume 2

[0085] Alcoholic chassis with Cyclodextrin Nelupure is added in the water and then mixed with the ethanol / fragrance oil / cyclodextrin solution. Full formula here below:

[0087] The samples were activated with water afterwards.Fresh 2h6h pre activation6h post activationAlcoholic chassis with Glucam:

[0088] Nelupure is added in the water and then mixed with the ethanol / fragrance oil / glucam solution. Full formula here below:Alcoholic chassis with Dermacryl 79 / Acrylates / Octylacrylamide Copolymer:

[0090] Nelupure is added in the water and then mixed with the ethanol / fragrance oil / cyclodextrin solution. Full formula here below:

[0091] Water-base fine fragrance:

[0092] Nelupure is added in the water base fine fragrance and mixed together with the Fragrance oil added afterwards. Full formula here below:

[0093] Results of the evaluations as per the protocol described above.

[0094] Waterproof evaluation Process:

[0095] "The following test is carried out to demonstrate the improved or enhanced retention of a fragrance of a composition of the present invention. In particular, the test measures the film forming and fixative effect on fragrance materials of a composition applied to an in-vitrosubstrate (e.g., glass slide). The properties are triggered after exposure to water. The amount of fragrance materials left on the substrate is evaluated by an expert panel."

[0096] A test composition comprising ethanol, water, one or more fragrances and Nelupure. A control composition to the test composition is made with ethanol, water, and the same one of more fragrances used in the test composition. A hotplate is set to a temperature of 32 °C. A glass slide, with dimensions of about 76×26 mm, is placed on the hotplate.50uL of the test or control composition is dispensed on a test area of the glass slide using a micropipette. The time at which this takes place is determined to be time zero (i.e., T = 0). The solution is left to evaporate on the glass slide at 32°C for 10min from T=0. The glass slide is then immersed in water having a temperature around 20-27°C for 4 minutes. The excess water is dabbed carefully avoiding the test area, and the glass slide is left to further dry for at 32°C on a hotplate for 2h. After 2h, the glass slide is again immersed in water having a temperature around 20-27°C for 4 minutes. The excess water is dabbed carefully avoiding the test area, and the glass slide is left to further dry until given to the expert panel for evaluation. The procedure is repeated for the "Fresh" time point. In this case, samples are given to the expert panel after the first immersion in water.Unperfumed water base used as a primer

[0098] Nelupure is added in unperfumed water base used as a primer, meaning it is first applied on skin and then a standard EDT is sprayed on top of the primer. Full formula here below:

[0087] Malodour is evaluated at the following times – note comments are directed towards a EDT solution with 5% Nelupure versus a EDT solution without Nelupure:

[0088] Indicia indicating malodour include: plastic; fatty / sweaty; mossy plastic notes. Example 3: Sensitizing potency using GARDskin

[0089] In addition to a longevity of a fragrance, users are concerned with skin sensitization potency of cosmetic fragrance formulations. Skin sensitization potency, i.e. Adverse Outcome Pathway, is measured herein with a GARDskin Dose-Response assay. This assay is a modification of the validated GARDskin protocol , OECD TGP 4.106, that incorporates dose response analysis.

[0090] The GARDskin assay employs a gene expression biomarker to identify skin sensitizers in a human myeloid dendritic-like cell line. The GARDskin Dose-Response investigates the GARDSKIN response values in a titrated range of multiple concentrations in a dose-response manner to find a concentration threshold required to induce a positive decision value. The concentration threshold is used to estimate the inherent sensitizing potency of a chemical. A low value indicates a high inherent skin sensitizing potency and a high valueindicates a low inherent skin sensitizing potency. The readout is a cDV0 value, describing the lowest concentration required to generate a positive classification. This value correlates with potency and can be used to rank test items by their relative sensitizing potency. This assay allows to evaluate mixtures and delivers continuous potency predictions, crucial point for comparing the modulator’s effect on the formulations’ skin sensitizing potency. One description of the method is described in this article: Quantitative assessment of sensitizing potency using a dose-response adaptation of GARDskin, R. Gradin et al., Scientific Reports, 18904 (2021).

[0091] The potential of a lotus flower extract to reduce the skin sensitizing potency of fragrance formulations containing different concentrations of fragrance oils was investigated using the GARDskin protocol.

[0092] The GARDskin Dose‐Response assay is based on the validated protocols of GARDskin (OECD TG 442E) and incorporates dose‐response measurements to derive continuous potency predictions. The readout of the assay corresponds to the lowest concentration required to exceed the binary classification threshold in GARDskin (DV≥ 0), referred to as the cDV0 value. This concentration is inversely correlated to skin sensitizing potency and can be used to predict a corresponding LLNA EC3 / human NESIL value in, for instance, micrograms per square centimeter, or be directly interpreted on its own to compare differences in skin sensitizing potency between investigated samples.

[0093] In this study, fragrance formulations with and without 2% Nelupure will be evaluated. The purpose of the study will be to investigate Nelupure’s efficacy for reducing skin sensitization. Table 1: TESTED SAMPLES:Results are shown below, with the sample containing Nelupure designated as MP23008, indicating a lower inherent skin sensitizing potency for a sample containing Nelupure.GARDskin DOSE RESPONSE METHOD:

[0094] The ability of each sample tested to modulate the sensitization potency will be measured using a GARDskin dose response method. The GARDskin dose response method is an in vitro test based on human dendritic-like cell cline, SenzaCell, a set of 200 genes known as the GARD Prediction Signature and a prediction model developed with machine learning technology. A summary of the GARDskin method is shown in FIG. 1. A test sample that includes at least one test substance is prepared and different concentrations of the test sample are grown in cells obtained from SenzaGen AB. A determination is made of the concentration of the test substance where cells react and 90 percent of cells survive. Then, a fresh batch of quality controlled cells are exposed to the determined concentration wherein cells react and 90% survive.

[0095] After exposure to the test sample, total RNA will be isolated from SenzaCells. Gene expression will be quantified and analyzed by pattern recognition using a machine learning algorithm based on a fixed set of reference samples. The genes that will be measured are well characterized and are related to several skin sensitization methanistic events. The resulting classification is that of a sensitizer or non-sensitizer. Additional information about the method can be found in a Nature article, “Quantitative assessment of sensitizing potency using a dose-response adaptation of GARDskin,” Nature, article no.18904 (2021).

[0096] It is hypothesized that the lowest concentration required to exceed the binary classification threshold (DV ≥ 0) and generate a positive classification, referred to as the cDV0concentration, would be informative of sensitizing potency. As such, the proposed dose– response measurements would also be aligned with common toxicological principles for potency assessments, where the response value (DV), the binary threshold (DV ≥ 0), and the derived cDV0concentrations may be viewed as an analogue to the response value (stimulation index (SI)), the binary threshold (SI ≥ 3), and the EC3 concentrations in the LLNA assay, respectively. Exemplary Embodiments. [1] The following exemplary embodiments are provided, the numbering of which is not to be construed as designating levels of importance: [2] Aspect 1 provides a fragrance composition comprising: a fragrance component; and an extract solution comprising:nymphaea coerulea flower extract; and nelumbo nucifera flower extract. [3] Aspect 2 provides the fragrance composition of Aspect 1, wherein the fragrance component present in an amount in a range of from about 0.04 wt% to about 40 wt% of the fragrance composition. [4] Aspect 3 provides the fragrance composition of any of Aspects 1 or 2, wherein the fragrance component present in an amount in a range of from about 0.5 wt% to about 30 wt% of the fragrance composition. [5] Aspect 4 provides the fragrance composition of any of Aspects 1-3, wherien the extract solution is present in an amount of at least 2 wt% of the fragrance composition. [6] Aspect 5 provides the fragrance composition of any of Aspects 1-4, further comprising a modulator component. [7] Aspect 6 provides the fragrance composition of Aspect 5, wherein the modulator component is present in an amount in a range of from about 0.04 wt% to about 40 wt% of the fragrance composition. [8] Aspect 7 provides the fragrance composition of any of Aspects 5 or 6, wherein the modulator component is present in a range of from about 0.1 wt% to about 27 wt%. [9] Aspect 8 provides the fragrance composition of any of Aspects 5-7, wherien the modulator component comprises: the compound according to Formula I:a repeating unit derived from the compound according to Formula II:a mixture thereof, wherein R1, R2, R3, R4, and R5, are independently chosen from -H, -OH, or substituted or unsubstituted (C1-C20)hydrocarbyl.

[0010] Aspect 9 provides the fragrance composition of any of Aspects 5-8, wherien the modulator component comprises pentylene glycol, polycitronellol, PPG-20 methyl glucose ether, methyl glucoside polyol, ethyl glucoside polyol, propyl glucoside polyol, or a mixture thereof.

[0011] Aspect 10 provides the fragrance composition of any of Aspects 5-9, wherien the modulator component is substantially non-odorous.

[0012] Aspect 11 provides the fragrance composition of any of Aspects 1-10, wherein the fragrance component comprises at least of: at least one low volatile fragrance material having a vapor pressure less than 0.001 Torr (0.000133 kPa) at 25 °C; at least one moderate volatile fragrance material having a vapor pressure in the range of 0.1 Torr to 0.001 Torr (0.0133 kPa to 0.000133 kPa) at 25 °C; and at least one high volatile fragrance material having a vapor pressure greater than 0.1 Torr (0.0133 kPa) at 25 °C.

[0013] Aspect 12 provides the fragrance composition of Aspect 11, wherein the at least one low volatile fragrance material is present in a range of from about 0 wt% to about 30 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 30 wt% to about 70 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 0 wt% to about 30 wt% of the fragrance component.

[0014] Aspect 13 provides the fragrance composition of any of Aspects 11 or 12, wherein the at least one low volatile fragrance material is present in a range of from about 30 wt% to about 70 wt% of the fragrance component; the at least one moderate volatile fragrance material is present at greater than about 30 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 0.1 wt% to about 30 wt% of the fragrance component.

[0015] Aspect 14 provides the fragrance composition of any of Aspects 11-13, wherein the at least one low volatile fragrance material is present in a range of from about 0.1 wt% to about 30 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 30 wt% to about 70 wt% of the fragrance component; and the at least one high volatile fragrance material is present at greater than about 30 wt% of the fragrance component.

[0016] Aspect 15 provides the fragrance composition of any of Aspects 11-14, wherein the at least one low volatile fragrance material is present in a range of from about 30 wt% to about 35 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 30 wt% to about 35 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 30 wt% to about 35 wt% of the fragrance component.

[0017] Aspect 16 provides the fragrance composition of any of Aspects 11-15, wherein the at least one low volatile fragrance material is present in a range of from about 0 wt% to about 35 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 40 wt% to about 60 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 0 wt% to about 30 wt% of the fragrance component.

[0018] Aspect 17 provides the fragrance composition of any of Aspects 11-16, wherein the at least one low volatile fragrance material is present in a range of from about 25 wt% to about 50 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 0 wt% to about 35 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 0.1 wt% to about 35 wt% of the fragrance component.

[0019] Aspect 18 provides the fragrance composition of any of Aspects 11-17, wherein the at least one low volatile fragrance material is present in a range of from about 25 wt% to about 60 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 0 wt% to about 35 wt% of the fragrance component; andthe at least one high volatile fragrance material is present in a range of from about 25 wt% to about 60 wt% of the fragrance component.

[0020] Aspect 19 provides the fragrance composition of any of Aspects 11-18, wherein the at least one low volatile fragrance material is present in a range of from about 25 wt% to about 60 wt% of the fragrance component; the at least one moderate volatile fragrance material is present in a range of from about 25 wt% to about 60 wt% of the fragrance component; and the at least one high volatile fragrance material is present in a range of from about 25 wt% to about 60 wt% of the fragrance component

[0021] Aspect 20 provides the fragrance composition of any of Aspects 11-19, wherein the high volatile fragrance material is chosen from any of the materials or combinations of materials listed in any of Tables 3A and 3B; the low volatile fragrance material is chosen from chosen from any of the materials or combinations of materials listed in any of Tables 1A and 1B; and the moderate volatile fragrance material is chosen from chosen from any of the materials or combinations of materials listed in any of Tables 2A and 2B.

[0022] Aspect 21 provides the fragrance composition of any of Aspects 1-20, further comprising a carrier.

[0023] Aspect 22 provides the fragrance composition of Aspect 21, wherein the carrier is an aqueous carrier, an organic carrier, or a mixture thereof.

[0024] Aspect 23 provides the fragrance composition of any of Aspects 21 or 22, wherein the organic carrier is ethanol, dipropylene glycol, benzyl benzoate, diethyl phthalate, isopropyl myristate, (C1-C20)alkyl, (C1-C20)alkenyl, (C1-C20)alkynyl, (C1-C20)hydroxyl, or mixtures thereof.

[0025] Aspect 24 provides the fragrance composition of any of Aspects 1-23, wherien the fragrance component is detectable for an amount of time longer than a corresponding fragrance composition that free of the extract solution.

[0026] Aspect 25 provides the fragrance composition of any of Aspects 1-24, wherien the fragrance component is more comfortable than a corresponding fragrance composition that free of the extract solution.

[0027] Aspect 26 provides the fragrance composition of any of Aspects 1-25, further comprising lactic acid, a hyaluronic acid, vitamin E, saccharomyces / xylinum / black tea ferment, diglucosyl gallic acid, adenosine, panthenol, dimethylmethoxy chromanol, alpha-glucan oligosaccharide, thermus thermophilus ferment, hydrolyzed hyaluronic acid, caffeine, acetyl di-peptide-1 cetyl ester, glycerin, niacinamide, swertia chirata extract, creatine, or a mixture thereof

[0028] Aspect 27 provides the fragrance composition of any of Aspects 1-26, further comprising niacinamide.

[0029] Aspect 28 provides the fragrance composition of any of Aspects 1-27, further comprising a chelant.

[0030] Aspect 29 provides the fragrance composition of Aspect 28, wherein the chelant comprises tetrasodium glutamate diacetate (GLDA), ethylenediaminetetraacetic acid (EDTA), ethylenediamine-N, N’-disuccinic acid (EDDS), NTA, EDDS, IDS, methylglycinediacetic acid (MGDA), L-glutamic acid N,N-diacetic acid (GLDA), ethylenediamine-N,N-diglutaric acid (EDDG), ethylenediamine-N,N’-dimalonic acid (EDDM), 3-hydroxy-2,2-iminodisuccinic acid (HIDS), 2-hydroxyethyliminodiacetic acid (HEIDA), methylglycine N,N-diacetic acid trisodium salt (MGDA), sodium gluconate, or a mixture thereof.

[0031] Aspect 30 provides the fragrance composition of any of Aspects 1-29, wherein the fragrance composition is more effective at reducing or eliminating malodor than a corresponding composition that is free of the extract solution.

[0032] Aspect 31 provides the fragrance composition of any one of Aspects 1-30, wherien modulation of the fragrance composition is increased relative to a corresponding composition that is free of the extract solution and comprises a cyclodextrin, PPG 20 methyl glucose ether, a chelator, or a mixture thereof.

[0033] Aspect 32 provides the fragrance composition of any one of Aspects 1-31, further comprising a cyclodextrin, a film former, or a mixture thereof.

[0034] Aspect 33 provides the fragrance composition of Aspect 32, wherein the at least one film former is chosen from anionic compounds or polymers, non-ionic compounds or polymers, amphoteric compounds or polymers, zwitterionic compounds or polymers, cationic compounds or polymers, proteins, viscosity modifiers, polyacrylates, polymethacrylates, polyacrylate copolymers, polymethacrylate copolymers, polyurethanes, polyamides, polyaminoamides, polyesters, polysaccharides, polyacrylamides, starches, gums, or mixtures thereof.

[0035] Aspect 34 provides the fragrance composition of any one of Aspects 32 or 33, wherien the cyclodexrin comprises an α-cyclodextrin, a β-cyclodextrin a gamma-cyclodextrin, or mixtures thereof.

[0036] Aspect 35 provides a formulation for decreasing skin sensitizing potency of the fragrance, comprising one or more: fragrance components; PPG 20 methyl glucose ether and ethanol; and an extract solution comprising: nymphaea coerulea flower extract; and nelumbo nucifera flower extract,

[0037] Aspect 36 provides a method comprising: applying a sebum reducing composition comprising a topically acceptable vehicle and the fragrance composition of any of Aspects 1-35.

[0038] Aspect 37 provides a method of treating skin with a component effective for functioning in a fragrance composition of Aspects 1-36, wherein the effectiveness of the component is determined by an assay for determining the levels of sebum, leptin, acetylcholine, or a mixture thereof in a sample that has been contacted with the extract or a composition including the extract.

[0039] Aspect 38 provides a method of inhibiting acetylcholinesterase, the method comprising contacting the fragrance composition of any of Aspects 1-37, with a keratinous tissue.

[0040] Aspect 39 provides a method of inhibiting a cholinergic receptor, the method comprising contacting the fragrance composition of any of Aspects 1-38, with a keratinous tissue.

Claims

CLAIMS What is claimed is:

1. A fragrance composition comprising: A solvent; a fragrance material; and an extract solution comprising: nymphaea coerulea flower extract; and nelumbo nucifera flower extract.

2. The fragrance composition of claim 1, wherein the solvent comprises water and / or ethanol 3. The fragrance composition of claim 2, wherein water is present at a ratio of at least 1:1 with the extract solution of the fragrance composition.

4. The fragrance composition of claim 1, wherein the ratio of fragrance material to extract solution is between 1:2 and 5:

1.

5. The fragrance composition of claim 1, wherein when the extract solution is present at a concentration of at least 5% and the fragrance material is comprised of a fougere accord, The fragrance composition exhibits a greater intensity as compared to a fragrance composition identical except for presence of the extract solution on application to human skin; 2 hours after application to human skin; and 6 hours after application to human skin.

6. The fragrance composition of claim 1, wherein when the extract solution is present at a concentration of at least 5% and the fragrance material is comprised of a citrus accord, The fragrance composition exhibits a greater intensity as compared to a fragrance composition identical except for presence of the extract solution 6 hours after application to human skin.

7. The fragrance composition of claim 1, wherein the fragrance material is present in an amount in a range of from about 0.04 wt% to about 40 wt% of the fragrance composition.

8. The fragrance composition of claim 1, wherein the composition exhibits greater retention of fragrance capacity when exposed to water as compared to an equivalent formula differing only in the presence of Nelupure.

9. The fragrance composition of claim 1,wherein the fragrance material is present in an amount in a range of from about 0.5 wt% to about 30 wt% of the fragrance composition.

10. The fragrance composition of claim 1, wherein the fragrance material is present in an amount in a range of from about 20%to about 40 wt% of the fragrance composition.

11. The fragrance composition of claim 1 wherein the extract solution is present in an amount of at least 4 wt% of the fragrance composition.

12. The fragrance composition of claim 1, further comprising a modulator component.

13. The fragrance composition of claim 12, wherein the modulator component is present in an amount in a range of from about 0.04 wt% to about 40 wt% of the fragrance composition.

14. The fragrance composition of claim 12, wherein the modulator component is present in a range of from about 0.1 wt% to about 27 wt%.

15. The fragrance composition ofclaim 13, wherien the modulator component comprises: the compound according to Formula I:a repeating unit derived from the compound according to Formula II:a mixture thereof, wherein R1, R2, R3, R4, and R5, are independently chosen from -H, -OH, or substituted or unsubstituted (C1-C20)hydrocarbyl.

16. The fragrance composition of claim 12, wherien the modulator component comprises pentylene glycol, polycitronellol, PPG-20 methyl glucose ether, methyl glucoside polyol, ethyl glucoside polyol, propyl glucoside polyol, or a mixture thereof.

17. The fragrance composition of claim 1, further comprising an organic carrier comprising ethanol, dipropylene glycol, benzyl benzoate, diethyl phthalate, isopropyl myristate, (C1- C20)alkyl, (C1-C20)alkenyl, (C1-C20)alkynyl, (C1-C20)hydroxyl, or mixtures thereof in a range of more than 10% of the fragrance composition. 18.. The fragrance composition of claim 1, wherein the fragrance component is detectable for an amount of time longer than a corresponding fragrance composition that is free of the extract solution.

19. The fragrance composition ofclaim 12, further comprising a chelant.

20. The fragrance composition of claim 1, further comprising a film former.

21. A primer composition comprising: Asolvent comprising water or ethanol and water; and an extract solution comprising: nymphaea coerulea flower extract; and nelumbo nucifera flower extract;With the proviso that the primer composition does not contain a fragrance material.

22. The fragrance composition of claim 1, wherein the fragrance composition is more effective at reducing or eliminating malodor than a corresponding composition that is free of the extract solution.

23. The fragrance composition of claim 22, wherein modulation of the fragrance composition is increased relative to a corresponding composition that is free of the extract solution and comprises a cyclodextrin, PPG 20 methyl glucose ether, a chelator, or a mixture thereof.

24. The fragrance composition of claim 23, further comprising a cyclodextrin, a film former, or a mixture thereof.

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