Combination of vitamins B1, B6 and B9 applied topically

Abstract

Combination of vitamins B1, B6 and B9 by topical route The present invention relates to the use of vitamins B1, B6 and B9, in particular by topical route, to combat the signs of aging and / or improve the quality of the skin. Figure for abstract: none

Description

Title of the invention: Association of vitamins B1, B6 and B9 by topical route

[0001] The present invention relates to the cosmetic use of vitamins B1, B6 and B9 to prevent and / or combat the signs of aging and / or improve the quality of the skin.

[0002] Human skin is made up of two compartments, namely a superficial compartment, the epidermis, and a deep compartment, the dermis.

[0003] The epidermis is composed mainly of three types of cells which are keratinocytes (majority), melanocytes and Langerhans cells, which play a primordial role in the immune response and in particular in antigenic presentation.

[0004] The dermis provides the epidermis with a solid support. It is also its nourishing element. It is mainly composed of fibroblasts and an extracellular matrix. It also contains leukocytes, mast cells and tissue macrophages. Finally, the dermis is crossed by blood vessels and nerve fibers.

[0005] The skin constitutes a barrier against external aggressions, in particular chemical and / or mechanical, and as such, a certain number of defense reactions against environmental factors (climate, UV, tobacco, pollution, etc.) and / or xenobiotics (such as certain medications) occur at its level: the skin is constantly subjected to stress, whether of intrinsic origin (chronological aging, hormonal deregulation, psychological stress, etc.) or external (UV, pollution, etc.).

[0006] All these stresses induce metabolic alterations at the cellular level, which result in significant physiological changes at the tissue level and more broadly at the organ level. These phenomena have direct consequences on the quality of the skin and increase the signs of aging, altering its ability to defend itself, regenerate and repair itself.

[0007] There is therefore a need for active ingredients capable of restoring the metabolic functions of skin cells and thus improving the quality of the skin and reducing the signs of aging.

[0008] The Applicant has now surprisingly identified the synergistic effect of the combination of vitamins B1, B6 and B9, in particular when applied topically to the skin, to restore the metabolic function of keratinocytes. These biological functions are described as promoting epidermal regeneration and function skin barrier. Thus, restoring the metabolic function of keratinocytes improves the quality and texture of the skin (therefore reducing its roughness), but also its suppleness and firmness, as well as reducing wrinkles and fine lines.

[0009] More broadly, these effects have the consequence of preventing and / or reducing the signs of aging, preventing and / or reducing senescence processes and improving skin regeneration.

[0010] The present invention therefore relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, to prevent and / or combat the signs of aging.

[0011] By "signs of aging" is meant all changes in the external appearance of the skin due to aging, whether chronobiological and / or photo-induced, such as, for example, wrinkles and fine lines, sagging skin, loose skin, thinning skin, lack of elasticity and / or tone of the skin.

[0012] By "preventing the signs of aging" is meant preventing or delaying the appearance of the signs of aging.

[0013] The present invention also relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, to restore the metabolic function of keratinocytes and / or stimulate their cellular regeneration.

[0014] In particular, the present invention relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, for improving the quality, texture and / or appearance of the skin, and / or for combating stress induced by the environment, preferably induced by UV rays and / or pollution. It also relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, for reducing skin roughness, and / or preventing and / or reducing fine lines and wrinkles. It also relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, to improve the suppleness, bounce and / or firmness of the skin.

[0015] The present invention also relates to the cosmetic use of (i) vitamin B1 or one of its salts, (ii) vitamin B6, one of its forms or one of its salts, and (iii) vitamin B9 or one of its salts, to prevent and / or reduce the signs of aging, and / or prevent and / or reduce the processes of senescence, and / or improve the regeneration of the skin.

[0016] The cosmetic uses according to the invention are typically topical, i.e. by application to the skin. In particular, the uses according to the invention are not for oral use.

[0017] The present invention also relates to a topical cosmetic composition comprising, in a physiologically acceptable medium:

[0018] (i) vitamin B1 or one of its salts,

[0019] (ii) vitamin B6, one of its forms or one of its salts, and

[0020] (iii) vitamin B9 or one of its salts, and

[0021] at least one additive chosen from pigmentary coloring materials and UV filters.

[0022] The present invention also relates to a method for making up and / or caring for keratin materials, comprising the application to the keratin materials of a topical composition according to the invention.

[0023] By “keratin materials” is meant the skin and / or the appendages; preferably the skin, preferably the skin of the face and / or the neck.

[0024] Vitamin B1, or thiamine, is a water-soluble vitamin from the B vitamin family. Animals find it in their diet, while it is synthesized by bacteria, plants and fungi. It is essential for the transformation of carbohydrates into energy by the Krebs cycle and is necessary for the proper functioning of the nervous system and muscles.

[0025] Vitamin B1 can be used as such, or in salt form, preferably thiamine monohydrate.

[0026] Preferably, thiamine monohydrate is used.

[0027] Vitamin B6 is a water-soluble vitamin from the B vitamin family, represented by three main forms: pyridoxine, pyridoxal and pyridoxamine. It is necessary for the proper functioning of the nervous and skin systems.

[0028] Pyridoxine, pyridoxal and pyridoxamine are forms which can be converted between them. They can be used as such, or in the form of salts, preferably the hydrochloride form.

[0029] Preferably, pyridoxine hydrochloride is used.

[0030] Vitamin B9, or folic acid, is a water-soluble vitamin from the B vitamin family. It is the metabolic precursor of the coenzyme tetrahydrofolate, involved in particular in the synthesis of nucleic bases and certain amino acids.

[0031] Vitamin B9 can be used as is, or in the form of folate ion (salt).

[0032] Preferably, folic acid is used.

[0033] Preferably, vitamins (i) to (iii) or their salts are formulated in a topical cosmetic composition comprising a physiologically acceptable medium.

[0034] This topical cosmetic composition is preferably liquid. It can be selected from creams, emulsions, lotions, dispersions, solutions, gels, balms and serums; or solid, preferably selected from masks and sticks. Preferably, it is not in solid form, which is less suitable for topical application. It can be used as a facial or body care product and / or as a skin makeup product.

[0035] Preferably, the topical cosmetic composition comprises:

[0036] from 0.001% to 20% by weight relative to the total weight of composition, preferably from 0.1% to 5% by weight, preferably from 1 to 5% by weight, of (i) vitamin B1 or one of its salts, and / or

[0037] from 0.001% to 10% by weight relative to the total weight of composition, preferably from 0.05% to 5% by weight, preferably from 0.1% to 1% by weight of (ii) vitamin B6, one of its forms or one of its salts, and / or

[0038] from 0.001% to 10% by weight relative to the total weight of composition, preferably from 0.05% to 5% by weight, preferably from 0.1% to 5%, preferably from 0.1% to 2% of (iii) vitamin B9 or one of its salts.

[0039] Preferably, the topical cosmetic composition comprises at least one ingredient chosen from surfactants, pigmentary coloring materials, oils, thickening polymers, UV filters, active ingredients and mixtures thereof.

[0040] As surfactants which can be used in the invention, mention may be made, for example, of anionic and non-ionic surfactants, and in particular fatty acid and polyethylene glycol esters such as PEG-20 stearate, and fatty acid and glycerin esters such as glyceryl stearate.

[0041] The pigmentary coloring materials are typically chosen from organic pigments, mineral pigments and nacres.

[0042] By "mineral pigment" is meant any pigment that meets the definition of the Ullmann encyclopedia in the inorganic pigment chapter. Among the mineral pigments useful in the present invention, mention may be made of zirconium or cerium oxides, as well as zinc, iron (black, yellow or red) or chromium oxides, manganese violet, ultramarine blue, chromium hydrate and ferric blue, titanium dioxide, metal powders such as aluminum powder and copper powder. The following mineral pigments may also be used: Ta2O5, Ti3O5, Ti2O3, TiO, ZrO2 in a mixture with TiO2, ZrO2, Nb2O5, CeO2, ZnS. In the context of the present invention, the mineral pigments are more particularly iron oxide and / or titanium dioxide.

[0043] The nacres can be chosen from pearlescent pigments, such as titanium mica coated with iron oxide, titanium mica coated with bismuth oxychloride, titanium mica coated with chromium oxide, titanium mica coated with an organic dye, as well as pearlescent pigments based on oxychloride of bismuth. It may also be mica particles on the surface of which are superimposed at least two successive layers of metal oxides and / or organic coloring materials. Examples of mother-of-pearl include natural mica coated with titanium oxide, iron oxide, natural pigment or bismuth oxychloride. Mother-of-pearl may more particularly have a yellow, pink, red, bronze, orange, brown, gold and / or coppery color or reflection.

[0044] By "organic pigment" is meant any pigment which meets the definition of the Ullmann encyclopedia in the organic pigment chapter. The organic pigment may in particular be chosen from nitroso, nitro, azo, xanthene, quinoline, anthraquinone, phthalocyanine, metal complex type compounds, isoindolinone, isoindoline, quinacridone, perinone, perylene, diketopyrrolopyrrole, thioindigo, dioxazine, triphenylmethane, quinophthalone.

[0045] The organic pigment(s) may be chosen, for example, from carmine, carbon black, aniline black, melanin, azo yellow, quinacridone, phthalocyanine blue, sorghum red, the blue pigments codified in the Color Index under the references CI 42090, 69800, 69825, 73000, 74100, 74160, the yellow pigments codified in the Color Index under the references CI 11680, 11710, 15985, 19140, 20040, 21100, 21108, 47000, 47005, the green pigments codified in the Color Index under the references CI 61565, 61570, 74260, the orange pigments codified in the Color Index under the references CI 11725, 15510, 45370, 71105, the red pigments codified in the Color Index under the references CI 12085, 12120, 12370, 12420, 12490, 14700, 15525, 15580, 15620, 15630, 15800, 15850, 15865, 15880, 17200, 26100, 45380, 45410, 58000, 73360, 73915, 75470, and the pigments obtained by oxidative polymerization of indole derivatives,phenolics as described in patent FR 2 679 771.,

[0046] As oils that can be used in the invention, mention may be made of mineral oils (vaseline oil), oils of vegetable origin (avocado oil, soybean oil), oils of animal origin (lanolin), synthetic oils (perhydrosqualene), silicone oils (cyclomethicone) and fluorinated oils (perfluoropolyethers). Fatty alcohols (cetyl alcohol), fatty acids, waxes (camauba wax, ozokerite) may also be used as fats.

[0047] As thickening polymers, mention may be made of hydrophilic gelling agents, and in particular carboxyvinyl polymers (carbomer), acrylic copolymers such as acrylate / alkylacrylate copolymers, polyacrylamides, polysaccharides, natural gums and clays; and lipophilic gelling agents, in particular modified clays such as bentones, metal salts of fatty acids, hydrophobic silica and polyethylenes.

[0048] As active ingredients, it is possible to use in particular moisturizing agents, depigmenting agents agents, anti-aging agents, anti-free radical agents and mixtures thereof. In the event of incompatibility, at least some of the active ingredients may be incorporated into spherules, in particular ionic or non-ionic vesicles and / or nanoparticles (nanocapsules and / or nanospheres), so that the mutually incompatible active ingredients are isolated from each other in the composition.

[0049] UV filters are typically chosen from organic UV filters and mineral UV filters.

[0050] The organic UV filters are in particular chosen from dibenzoyl methane derivatives such as avobenzone, Terephthalylidene Dicamphor Sulfonic Acid manufactured under the name "MEXORYL SX" by CHIMEX, bis-benzoazolyl derivatives as described in EP 669 323, and US 2,463,264 and more particularly the compound Disodium Phenyl Dibenzimidazole Tetra-sulfonate sold under the trade name "NEO HELIOPAN AP" by Haarmann and REIMER, p-aminobenzoic acid (PABA) derivatives such as PABA, Glyceryl PABA, and PEG-25 PABA sold under the name "UVINUL P25" by BASF, Phenylbenzimidazole Sulfonic Acid sold in particular under the trade name "EUSOLEX 232" by MERCK, ferulic acid, salicylic acid, DEA methoxycinnamate, Benzylidene Camphor Sulfonic Acid manufactured under the name "MEXORYL SL" by CHIMEX, and Camphor Benzalkonium Methosulfate manufactured under the name "MEXORYL SO" by CHIMEX, benzophenone derivatives containing at least one sulfonic radical,such as in particular Benzophenone-4 sold under the trade name "UVINUL MS40" by BASF, Benzophenone-5 and Benzophenone-9.,

[0051] Mineral UV filters are notably chosen from titanium dioxide, zinc oxide

[0052] and cerium oxide.

[0053] The present invention also relates to a topical cosmetic composition comprising, in a physiologically acceptable medium:

[0054] (i) vitamin B1 or one of its salts,

[0055] (ii) vitamin B6, one of its forms or one of its salts, and

[0056] (iii) vitamin B9 or one of its salts, and

[0057] at least one additive chosen from pigmentary coloring materials and UV filters.

[0058] The pigmentary coloring matter is in particular described above, and chosen from organic pigments, mineral pigments and nacres. Preferably, the topical cosmetic composition comprises at least 1% by weight, preferably at least 3% by weight, preferably at least 5% by weight relative to the total weight of the composition, of at least one pigmentary coloring matter.

[0059] The UV filter is notably described above, and chosen from organic UV filters and mineral UV filters. Preferably, the topical cosmetic composition comprises at least 1% by weight, preferably at least 3% by weight, preferably at least 5% by weight relative to the total weight of the composition, of at least one UV filter.

[0060] Preferably, in this topical cosmetic composition, (i) vitamin B1 or one of its salts is thiamine monohydrate, and / or (ii) vitamin B6, one of its forms or one of its salts is chosen from pyridoxine, pyridoxal, pyridoxamine and their salts, preferably pyridoxine hydrochloride, and / or (iii) vitamin B9 or one of its salts is folic acid.

[0061] Preferably, the topical cosmetic composition comprises:

[0062] from 0.001% to 20% by weight relative to the total weight of composition, preferably from 0.1% to 5% by weight, preferably from 1 to 5% by weight, of (i) vitamin B1 or one of its salts, and / or

[0063] from 0.001% to 10% by weight relative to the total weight of composition, preferably from 0.05% to 5% by weight, preferably from 0.1% to 1% by weight of (ii) vitamin B6, one of its forms or one of its salts, and / or

[0064] from 0.001% to 10% by weight relative to the total weight of composition, preferably from 0.05% to 5% by weight, preferably from 0.1% to 5%, preferably from 0.1% to 2% of (iii) vitamin B9 or one of its salts.

[0065] The present invention also relates to a method for making up and / or caring for keratin materials, comprising the application to the keratin materials of a topical composition according to the invention.

[0066] The invention is now illustrated by the following examples.

[0067] The legends of the figures are as follows:

[0068] [Fig-1] [Fig.l] presents the effects of Vitamins Bl, B6, B9 and their association on the expression of transcripts (mRNA) involved in keratinocyte migration - Analysis by RT-qPCR.

[0069] [Fig.2] [Fig.2] presents the effects of Vitamins Bl, B6, B9 and their association on the expression of transcripts (mRNA) involved in the migration of keratinocytes - Analysis by RT-qPCR.

[0070] [Fig.3] [Fig.3] shows the effects of Vitamins Bl, B6, B9 and their association on the protection of keratinocytes against oxidative stress with H2O2 - Measurement of NAD concentration. Non-stimulated control = non-stimulated control; stimulated control = stimulated control.

[0071] [Fig.4] [Fig.4] shows the effects of Vitamins Bl, B6, B9 and their association on the protection of keratinocytes against UV stress - Measurement of NAD concentration. Non-stimulated control = non-stimulated control; stimulated control = stimulated control. Examples

[0072] Example 1: Evaluation of the effectiveness on the migration and proliferation of keratinocytes

[0073] Principle:

[0074] The aim of this study is to evaluate the effects of vitamins on the expression of transcripts (mRNA) involved in the migration and proliferation of keratinocytes, key processes of re-epithelialization, by RT-qPCR.

[0075] Vitamins B1, B6 and B9 were tested alone or in combination, respectively at 2, 0.3 and 0.1 mg / ml.

[0076] The different markers studied are: CEACAM6, RAC2, RASAI, KRT19 and HBEGF.

[0077] Materials and methods:

[0078] The keratinocytes were seeded in 48-well plates and cultured for 72 hours with renewal of the culture medium after 24 hours of incubation. The medium was then renewed with culture medium containing or not (Control), Vitamin Bl at 2 mg / ml, or Vitamin B6 at 0.3 mg / ml, or Vitamin B9 at 0.1 mg / ml or the combination of Vitamins Bl, B6 and B9 respectively at 2, 0.3 and 0.1 mg / ml. The cells were then incubated for an additional 24 hours. All conditions were carried out in n=3.

[0079] At the end of the treatments, the culture media were removed and the cells were rinsed twice in PBS (w / o CaCl2, w / o MgCl2). Total RNA was then isolated using a magnetic bead extraction kit and according to the supplier's recommendations (MagMAXTM-96 Total RNA Isolation Kit, Ambion). RNA quantification and quality control were analyzed using the Labchip GX (Perkin Elmer).

[0080] The expression of the selected transcripts was analyzed by 2-step quantitative PCR. First, cDNAs were reverse transcribed from RNAs using the Quantitect® Reverse Transcription Kit (QIAGEN) and according to the supplier's recommendations. Quantitative PCR experiments were then performed using a LightCycler® 480 Real-Time PCR System in a 384-well plate (Roche) and according to the SYBR®Green incorporation technique (Roche).

[0081] Results and conclusions:

[0082] The results are expressed as modulation factor (Fc, fold change) compared to the untreated control after normalization of the relative expressions compared to the expression of housekeeping genes (GAPDH and RPL13A). The expression of a gene is considered to be stimulated when it is multiplied by at least 1.5.

[0083] The results show that Vitamin B1 tested at 2 mg / ml stimulates weakly but significantly the expression of RAC2, without modulating the expression of others markers, that Vitamin B6 tested at 0.3 mg / ml stimulates the expression of CEACAM6, RAC2 and KRT19 and finally, that Vitamin B9 tested at 0.1 mg / ml stimulates the expression of CEACAM6, RAC2 and KRT19 (Figures 1 & 2). Surprisingly and unexpectedly, the combination of the 3 vitamins Bl, B6 and B9 tested respectively at 2, 0.3 and 0.1 mg / ml synergistically stimulates the expression of all the markers CEACAM6, RAC2, RASAI, KRT19 and HBEGF. The modulation factors are very clearly and significantly higher than the sum of the effects observed with the vitamins tested alone and only the combination of the 3 vitamins can significantly stimulate the expression of the RASAI and HBEGF markers. All these elements thus demonstrate the synergistic activity of the mixture. The CEACAM6 markers (Akihiko F, ​​Kiyofumi E, Yumi H, Masahide K, Masatoshi J and Hironobu I. CEA (Carcinoembryonic Antigen) and CEACAM6 (CEA-Related Cell Adhesion Molecul 6) are expressly sed in Psoriasis Vulgaris.The Open Dermatology Journal, 2013, 7, 47-52), RAC2 (Ridley AJ. Rho GTPase signaling in cell migration. Curr Opin Cell Biol. 2015 Oct;36:103-12) and RASAI (Fitsialos G, Chassot AA, Turchi L, Dayem MA, LeBrigand K, Moreilhon C, Meneguzzi G, Buscà R, Mari B, Barbry P, Ponzio G. Transcriptional signature of epidermal keratinocytes subjected to in vitro scratch wounding reveals selective roles for ERK1 / 2, p38, and phosphatidylinositol 3-kinase signaling pathways. J Biol Chem. 2007 May 18;282(20): 15090-102) encode proteins involved in keratinocyte migration, while the markers KRT19 (Michel M, Tôrôk N, Godbout MJ, Lussier M, Gaudreau P, Royal A, Germain L. Keratin 19 as a bio-chemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomical sites, and their number varies with donor age and culture stage. J Cell Sci.1996 May; 109 (Pt 5):1017-28) and HBEGF (Yoshida A, Kanno H, Watabe D, Akasaka T, Sawai T. The role of heparin-binding EGF-like growth factor and amphiregulin in the epidermal proliferation of psoriasis in cooperation with TNFalpha. Arch Dermatol Res. 2008 Jan;300(1):37-45) are involved in keratinocyte proliferation.

[0084] The combination of vitamins BL B6 and B9 is therefore of interest in skin aging by promoting epidermal renewal, the thickness of the epidermis decreasing with age (Branchet MC, Boisnic S, Frances C, Robert AM. Skin thickness changes in normal aging skin. Gerontology. 1990;36(l):28-35. doi: 10.1159 / 000213172. PMID: 2384222). and in skin regeneration and healing. keratinocyte migration and proliferation being key mechanisms in the re-epithelialization process (Patricia Rousselle, Fabienne Braye, Guila Dayan. Re-epithelialization of adult skin wounds: cellular mechanisms and therapeutic strategies. Advanced Drug Delivery Reviews, Elsevier, 2019, 146, pp.344-365. ffl0.1016 / j.addr.2018.06.019ff. ffhal-03035503f).

[0085] Example 2: Evaluation of the effectiveness on NAD production under conditions of stress induced by UV or by H2O2 treatment

[0086] Principle:

[0087] The aim of this study is to evaluate the effects of vitamins on the protection of keratinocytes against NAD depletion induced by oxidative stress following UV irradiation or following H2O2 treatment.

[0088] Vitamins B1, B6 and B9 were tested alone or in combination, respectively at 2, 0.3 and 0.1 mg / ml.

[0089] The marker studied is a quantification of the NAD concentration relative to the total protein concentration.

[0090] Materials and methods:

[0091] Keratinocytes were seeded into 12-well plates and cultured for 72 hours in culture medium.

[0092] - Conditions stimulated by H2O2:

[0093] The medium was then replaced with medium containing or not (control), Vitamin Bl at 2 mg / ml, or Vitamin B6 at 0.3 mg / ml, or Vitamin B9 at 0.1 mg / ml or the combination of Vitamins Bl, B6 and B9 respectively at 2, 0.3 and 0.1 mg / ml and the cells were pre-incubated for 24 hours. After the pre-incubation, the medium was collected and the cells were stimulated for 2 hours with 250 pM H2O2. The medium was then replaced with the previously collected medium and the cells were incubated for an additional 48 hours. All experimental conditions were carried out in n=3.

[0094] - UV irradiation conditions:

[0095] The medium was then replaced with medium containing or not (control), Vitamin Bl at 667 pg / ml, or Vitamin B6 at 100 pg / ml, or Vitamin B9 at 33.3 pg / ml or the combination of Vitamins Bl, B6 and B9 respectively at 667, 100 and 33.3 pg / ml and the cells were pre-incubated for 24 hours. After the pre-incubation, the medium was collected and replaced with irradiation medium and the cells were irradiated with UVB at 275 mJ / cm2 (+ UVA at 2.1 J / cm2). The lamp used was a SOL500 solar simulator equipped with an H2 filter (Dr. Hônle, AG). The medium was then replaced with the previously collected medium and the cells were incubated for an additional 48 hours. All experimental conditions were carried out in n=3.

[0096] At the end of the treatments, the media were removed and the amount of NAD was quantified using a specific assay kit and according to the supplier's recommendations (BioVision, #K337-100). The amount of protein in each culture well was also quantified using a specific kit and always according to the supplier's recommendations (BIO-RAD, #500-0116).

[0097]

[0098] A percentage of protection against stress was calculated according to the formula: [Math.l]

[0099]

[0100]

[0101] LVAÜl - WAD! Protection (%) = 100 x ............ • ^Concrete mstimulated ' ' ^Stimulated witness [NAD]Treatment '■ NAD concentration (pmol / mg protein) under treated conditions, [NAD]Stimulated control: NAD concentration (pmol / mg of protein) in controls stimulated by H2O2 or UVs, [NAD]Unstimulated control '■ NAD concentration (pmol / mg protein) under unstimulated conditions.

[0102] Results and conclusions:

[0103] The results show that both stresses, UV and H2O2, induced a net decrease in the intracellular concentration of NAD (Figures 3 and 4). Under these conditions, only Vitamin B6, tested alone at 0.3 mg / ml, significantly protected keratinocytes from the stress induced by H2O2 treatment ([Fig.4]). The other vitamins, B1 and B9, tested alone had no significant effect. Surprisingly and unexpectedly, the combination of the 3 vitamins Bl, B6 and B9 tested respectively at 2, 0.3 and 0.1 mg / ml very effectively protected keratinocytes from the stress induced by H2O2 treatment ([Fig.4]). The NAD concentration was even higher than that measured in the unstimulated control. Similarly, the combination of the 3 vitamins Bl, B6 and B9 tested respectively at 667, 100 and 33.3 pg / ml effectively protected keratinocytes from stress induced by UV irradiation ([Fig.4]).In both cases, the effects of the mixture were significantly greater than the sum of the effects of the vitamins tested alone, demonstrating the synergistic activity between the 3 vitamins. NAD plays a central role in energy metabolism and is a co-substrate of many enzymes involved in modifying the signs of aging.

[0104] Knowing that increasing NAD helps slow down aging (Conlon NJ. The Role of NAD+ in Regenerative Medicine. Plast Reconstr Surg. 2022 Oct 1; 150(4 Suppl):41S-48S), these results demonstrate the interest of the combination of BL vitamins B6 and B9 to fight against the signs of aging.

Claims

Claims

1. Topical cosmetic composition comprising, in a physiologically acceptable medium: from 0.001% to 20% by weight relative to the total weight of composition of (i) vitamin B1 or one of its salts, from 0.001% to 10% by weight relative to the total weight of composition of (ii) vitamin B6, one of its forms or one of its salts, and from 0.001% to 10% by weight relative to the total weight of composition of (iii) vitamin B9 or one of its salts, and at least one additive chosen from pigmentary coloring matters and UV filters.

2. Composition according to claim 1, characterized in that (i) vitamin B1 or one of its salts is thiamine monohydrate, and / or (ii) vitamin B6, one of its forms or one of its salts is chosen from pyridoxine, pyridoxal, pyridoxamine and their salts, preferably pyridoxine hydrochloride, and / or (iii) vitamin B9 or one of its salts is folic acid.

3. Composition according to claim 1 or 2, characterized in that it comprises: from 0.1% to 5% by weight relative to the total weight of composition, preferably from 1 to 5% by weight, of (i) vitamin B1 or one of its salts, and / or from 0.05% to 5% by weight relative to the total weight of composition, preferably from 0.1% to 1% by weight of (ii) vitamin B6, one of its forms or one of its salts, and / or from 0.05% to 5% by weight relative to the total weight of composition, preferably from 0.1% to 5%, preferably from 0.1% to 2% of (iii) vitamin B9 or one of its salts.

4. Cosmetic use of a composition according to one of claims 1 to 3, to prevent and / or combat the signs of aging.

5. Cosmetic use of a composition according to one of claims 1 to 3, for restoring the metabolic function of keratinocytes and / or stimulating their cellular regeneration.

6. Use according to claim 4 or 5, for improving the quality, texture and / or appearance of the skin, and / or for combating stress induced by the environment, preferably induced by UV and / or pollution.

7. Use according to claim 4 or 5, for reducing the roughness of the skin, and / or preventing and / or reducing fine lines and wrinkles.

8. Use according to claim 4 or 5, for improving the suppleness, bounce and / or firmness of the skin.

9. Use according to claim 4 or 5, for preventing and / or reducing the signs of aging, and / or preventing and / or reducing senescence processes, and / or improving skin regeneration.

10. Use according to one of claims 4 to 9, characterized in that the topical cosmetic composition is liquid, preferably chosen from creams, emulsions, lotions, dispersions, solutions, gels, balms and serums; or solid, preferably chosen from masks and sticks.

11. Method for making up and / or caring for keratin materials, comprising the application to the keratin materials of a topical composition according to one of claims 1 to 3.

Images