COLLAGEN TRIPEPTIDE (GLY-XY) MICROPARCEL PREPARATION SYSTEM

Abstract

SYSTEM FOR PREPARING COLLAGEN TRIPEPTIDE (GLY-XY) MICROPARTICLES The present invention relates to a system for preparing collagen tripeptide (Gly-XY) microparticles, comprising a pretreatment portion (10) for extracting animal skin gelatin from fresh animal skin, a reaction portion (20) for hydrolyzing the animal skin gelatin with enzymes to obtain collagen tripeptide (Gly-XY), and a refining portion (30) for refining the collagen tripeptide (Gly-XY) to form collagen tripeptide (Gly-XY) microparticles having a coated particle structure. Figure to be published with the abstract: Figure 1

Description

Title of the invention: SYSTEM FOR PREPARING COLLAGEN TRIPEPTIDE (GLY-XY) MICROPARTICLES technical field

[0001] The present application relates to a food manufacturing system, in particular a system for preparing collagen tripeptide microparticles (Gly-XY). BACKGROUND

[0002] Collagen is an important protein in the human body, generally one of the constituents of skin, bones, cartilage, ligaments, blood vessels, tendons, and connective tissues. However, with age, collagen production decreases and manifests itself externally, for example, by the appearance of wrinkles on the skin.

[0003] To address the aforementioned problem, related collagen products are sold or available on the market, and are generally in powder, lozenge, or liquid form. Among these, collagen products in lozenge form are taken orally to supplement collagen absorption.

[0004] However, if collagen products are stored under undesirable environmental conditions, such as prolonged exposure to direct sunlight or a high-temperature environment, deterioration is likely to occur, meaning that the structure of the collagen may be disrupted, which in turn reduces activity.

[0005] Furthermore, conventional methods for preparing collagen for production mainly involve procedures such as obtaining raw materials (such as fish skin or scales), chopping, and hydrolysis. Various types and quantities of equipment are required for the preparation process, and each device operates independently. If the equipment is controlled manually, errors often occur, leading to variations in quality between batches of collagen products.

[0006] Based on the above description, it can be seen that conventional collagen products have the disadvantages of being difficult to store and of having unstable quality, which are the problems that the practitioners concerned wish to improve urgently.

[0007] The collagen tripeptide microparticles (Gly-XY) produced by the system of the present invention have a high content of active peptide ingredients and can maintain their functional activity to a certain extent, as well as to extend the lifespan of the product obtained through this system. SUMMARY

[0008] Therefore, the main objective of the present application is to provide a system for preparing collagen tripeptide microparticles (Gly-XY), capable of providing automated operations to ensure the quality of the finished product.

[0009] Accordingly, the invention relates to a system for preparing collagen tripeptide microparticles (Gly-XY) which comprises:

[0010] a pretreatment part for receiving a material and pretreating the material to form an intermediate product by acid treatment or alkali treatment, the material being fresh animal skin and the intermediate product comprising gelatin;

[0011] a reaction portion disposed after the pretreatment portion to receive the intermediate product and add an enzyme to react with the intermediate product, thereby producing a collagen tripeptide (Gly-XY), the enzyme being a protease; and

[0012] a refining portion disposed after the reaction portion to receive and refine the collagen tripeptide (Gly-XY) in order to form the collagen tripeptide (Gly-XY) microparticles,

[0013] the pretreatment part, the reaction part and the refining part can be connected to each other by pipes.

[0014] Advantageously, the material can be fresh fish skin and the intermediate product can be fish skin gelatin.

[0015] The pretreatment part may include:

[0016] a grinding unit for receiving and chopping fresh animal skin to form a ground product;

[0017] a screening unit arranged after the grinding unit to receive and screen the ground product;

[0018] a reaction processing unit disposed after the screening unit to receive the screened and crushed product and to add an acid or a base for the reaction in order to obtain a decomposed mixture; and

[0019] an extraction unit disposed after the reaction processing unit to receive and extract the decomposed mixture in order to obtain the intermediate product.

[0020] The reaction unit may have a stirring unit, the stirring unit comprising:

[0021] a reservoir intended to receive the enzyme and the intermediate product; and

[0022] an agitation part disposed in the tank.

[0023] The refining portion may include:

[0024] a concentration and sterilization unit for performing a concentration and sterilization procedure on collagen tripeptide (Gly-XY);

[0025] a drying unit arranged after the concentration and sterilization unit for to heat and dry the concentrated and sterilized collagen tripeptide (Gly-XY) to produce a collagen tripeptide (Gly-XY) powder; and

[0026] a coating unit disposed after the drying unit to receive the collagen tripeptide powder (Gly-XY), and to mix and process the collagen tripeptide powder (Gly-XY) with a base material to form the collagen tripeptide microparticles (Gly-XY). Brief description of the drawings

[0027] [Fig.1] is a schematic diagram of the system for preparing collagen tripeptide microparticles (Gly-XY) shown in a preferred embodiment of the present invention.

[0028] [Fig.2] is a block diagram of the collagen tripeptide microparticle preparation system (Gly-XY) shown in a preferred embodiment of the present invention.

[0029] [Fig.3] is a flowchart of the system for preparing collagen tripeptide microparticles (Gly-XY) presented in a preferred embodiment of the present invention.

[0030] [Fig.4] is a partial cross-sectional view of collagen tripeptide microparticles (Gly-XY) in a preferred embodiment of the present invention.

[0031] [Fig.5] shows the results of experiments on the absorption capacity in the digestive tract of rats in each group.

[0032] [Fig.6] shows the results of collagen production experiments by human dermal fibroblasts in each group in the absence of vitamin C.

[0033] [Fig.7] shows the results of collagen production experiments by human dermal fibroblasts in each group in the presence of vitamin C.

[0034] [Fig.8] shows the results of experiments on the production of hyaluronic acid by human dermal fibroblasts in each group.

[0035] [Fig.9] shows the results of collagen synthesis experiments in rats in each group.

[0036] [Fig. 10] shows the results of collagen production experiments by human osteoblasts in each group.

[0037] [Fig. 11] shows the results of experiments on calcium synthesis by human osteoblasts in each group, as measured by the absorbance value at 562 nm.

[0038] [Fig. 12] shows the results of bone strength assessment experiments carried out on rats in each group.

[0039] [Fig. 13] shows the results of wound tendon healing experiments carried out on rats in each group.

[0040] [Fig. 14] shows the results of experiments on the healing of an injured tendon carried out on rats in each group.

[0041] [Fig. 15] shows the results of cartilage tissue healing experiments injuries were observed on rabbits in each group.

[0042] [Fig. 16] shows the results of experiments carried out on patients suffering from a degenerative joint disease of the knee in each group. DETAILED DESCRIPTION

[0043] First, referring to Figures 1-3, it can be seen that a system for preparing collagen tripeptide microparticles (Gly-XY) provided in a preferred embodiment of the present invention mainly comprises a unidirectional processing path which includes a pretreatment section 10, a reaction section 20, and a refining section 30 successively for mass production. The pretreatment section 10, the reaction section 20, and the refining section 30 are connected to each other by conduits, so as to achieve the objective of automated operation.

[0044] The pretreatment unit 10 comprises a grinding unit 11, a screening unit 12, a reaction processing unit 13, and an extraction unit 14, which are connected in succession. The grinding unit 11 is used to receive and grind fresh fish skin to form a ground product. The screening unit 12 is placed after the grinding unit 11 to receive and screen the ground product. The reaction processing unit 13 is placed after the screening unit 12 to receive the screened and ground product and add an acid or a base for the reaction to obtain a decomposed mixture, the acid being hydrochloric acid and the base being a liquid alkali. The extraction unit 14 is placed after the reaction processing unit 13 to receive and extract the decomposed mixture to obtain an intermediate product.

[0045] In other words, the pretreatment unit 10 is used to extract and separate the intermediate product from fresh fish skin by acid or alkali treatment, the pH value of the acid treatment being 3.5–5.0, and the pH value of the alkali treatment being 10.5–12.0. More specifically, water can be used as the solvent, and then hydrochloric acid or a liquid alkali can be added to adjust the pH value to meet the process requirements; then the ground product is placed in the solvent for reaction. After the reaction, the decomposition mixture is obtained for extraction, and then the acidic or alkali solution is withdrawn to obtain the intermediate product.

[0046] In this embodiment, the intermediate product is fish skin gelatin. Furthermore, the pretreatment unit 10 also includes a grinding unit. 11A between the grinding unit 11 and the screening unit 12, which can grind solid particles of the ground product from the grinding unit 11 into smaller particles.

[0047] The reaction section 20 is arranged after the pretreatment section 10 to receive the intermediate product and to add an enzyme to react with the intermediate product, thereby producing a collagen tripeptide (Gly-XY), the enzyme being a protease, such as an alcalase. In this embodiment, the reaction section 20 includes a stirring unit 21, the stirring unit comprising a reservoir for receiving the enzyme, the intermediate product and a liquid, and a stirring element disposed in the reservoir for uniformly mixing the enzyme and the intermediate product in the reservoir and distributing them in the liquid.

[0048] The refining section 30 comprises a concentration and sterilization unit 31, a drying unit 32, and a coating unit 33. Among these, the concentration and sterilization unit 31 is used to receive the collagen tripeptide (Gly-XY) and perform a concentration and sterilization procedure on it. The drying unit 32 is placed after the concentration and sterilization unit 31 to heat and dry the concentrated and sterilized collagen tripeptide (Gly-XY) in order to produce a collagen tripeptide (Gly-XY) powder.The coating unit 33 is placed after the drying unit 32 to receive the collagen tripeptide powder (Gly-XY) and by a granulation and spray-drying process to mix the collagen tripeptide powder (Gly-XY) with a base material in order to form the collagen tripeptide microparticles (Gly-XY), the base material being an adhesive material such as starch.

[0049] Furthermore, the tripeptide collagen microparticles (Gly-XY) 40 have a diameter between 60 µm and 1000 µm, and further comprise a core 41 and a coating layer 42, as illustrated in [Fig. 4]. The coating layer can be made of the base material.

[0050] In addition, core 41 comprises the collagen tripeptide, which is composed of a Gly-XY sequence, in which Gly is glycine, and X and Y are each one of the following amino acids: glycine, asparagine, glutamate, alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, tyrosine, aspartate, histidine, methionine, arginine, serine, threonine, lysine, glutamine, cysteine, proline, hydroxyproline and the like.

[0051] The collagen tripeptide of the present invention is prepared from fish skin. However, in other embodiments, the collagen tripeptide can also be extracted from other animal tissues, such as pigskin, cowhide, or chicken feet. Since the molecular mass of the collagen tripeptide is 300 daltons (Da), or one-thousandth of that of conventional collagen, it is more It is easily absorbed by the human intestine and can achieve optimal absorption and utilization rates in the human body. In other words, the tripeptide collagen microparticles (Gly-XY) prepared by the system of the present invention can improve the absorption and utilization rates of collagen in the human body, and can therefore effectively improve consumer positive perception and repurchase rates.

[0052] Thanks to the aforementioned structural design, the specific implementation steps of the present invention are as follows:

[0053] Step A: subject fresh fish skin successively to grinding, screening, reaction treatment and extraction, in order to obtain fish skin gelatin;

[0054] Step B: break down fish skin gelatin with enzymes to produce a collagen tripeptide (Gly-XY); and

[0055] Step C: subject the collagen tripeptide (Gly-XY) to concentration, sterilization, drying and coating to obtain collagen tripeptide (Gly-XY) microparticles.

[0056] The following data are actual experimental data according to the present invention. Example 1: Absorption in the digestive tract

[0057] Figure 5 shows the results of experiments on rats. The rats were divided into an experimental group A and a control group A under restriction conditions. The experimental group A comprises rats fed with the collagen tripeptide of the present invention, and the control group A comprises rats fed with conventional amino acids.

[0058] As shown in [Fig.5], the amount of collagen tripeptide absorbed was greater than that of amino acids in a predetermined time period. Example 2: Collagen Production

[0059] First, experiments were carried out in the absence of vitamin C with human dermal fibroblasts and different amounts of collagen tripeptide. As shown in [Fig. 6], the addition of collagen tripeptide helped to increase collagen production.

[0060] Secondly, experiments were carried out in the presence of vitamin C with human dermal fibroblasts and different amounts of collagen tripeptide. As shown in [Fig.7], the addition of collagen tripeptide and vitamin C significantly increased collagen production. Example 3: Hyaluronic acid production

[0061] Figure 8 shows the results of experiments with dermal fibroblasts humans. Experimental group C received collagen tripeptide, and experimental group C received glucosamine. Control group C received only water. As shown in [Fig. 8], the amount of hyaluronic acid produced in experimental group C was greater than that in experimental group C, and the amount of hyaluronic acid produced in experimental group C was greater than that in control group C. Example 4: Collagen Synthesis

[0062] Figure 9 shows the results of experiments on rats. Experimental group D received collagen tripeptide, experimental group D' received a mixture of amino acids, experimental group D" received unbroken collagen, and experimental group D'" received a conventional collagen peptide. The control group D received none of these components. As shown in Figure 9, the hydroxyproline content in experimental group D was higher than in the other experimental groups. Thus, the collagen tripeptide of the present invention can promote collagen synthesis. Example 5: Collagen Production

[0063] Figure 10 shows the results of experiments with human osteoblasts. Experimental group E received 10 µg / ml of the collagen tripeptide, and control group E received no collagen tripeptide. As shown in Figure 10, the collagen content in experimental group E was higher than that in control group E. Thus, the collagen tripeptide of the present invention can promote collagen production in bones. Example 6: Calcium Synthesis

[0064] Figure 11 shows the results of experiments with human osteoblasts under different amounts of collagen tripeptide. As can be seen, the higher the amount of collagen tripeptide added, the greater the calcium synthesis. Example 7: Assessing bone strength

[0065] Figure 12 shows the experimental results of rats fed with different amounts of collagen tripeptide. As can be seen, the higher the amount of collagen tripeptide supplied, the greater the weight ratio applied.

[0066] Example 8: Promoting the healing of a tendon injury

[0067] Figures 13 and 14 show the results of experiments carried out on rats whose Achilles tendons were severed and the tissues were fed different amounts of collagen tripeptide.

[0068] As shown in Figures 13 and 14, the greater the amount of collagen tripeptides The higher the supply, the higher the collagen content ratio and the higher the tendon tension ratio.

[0069] Example 9: Healing of damaged cartilage tissue

[0070] Figure 15 shows the results of experiments on rabbits, obtained by observing changes in the surface of the cartilage tissue of an injured knee joint after four weeks. Experimental group F received the collagen tripeptide, experimental group F' received conventional collagen, and control group F received neither the collagen tripeptide nor conventional collagen.

[0071] As shown in [Fig. 15], compared with the other groups, experimental group F showed a greater reduction in the injured area.

[0072] Example 10: Improvement of a degenerative joint disease of the knee

[0073] Figure 16 shows the changes observed during clinical trials conducted on patients with degenerative joint disease of the knee over a period of 10 weeks. The experimental group G received 4 g of collagen tripeptide and the control group G did not receive collagen tripeptide.

[0074] As shown in [Fig.16], the experimental group G experienced a greater improvement in knee pain than the control group G.

Claims

Demands

1. A system for preparing collagen tripeptide microparticles (Gly-XY) characterized in that it comprises: a pretreatment part (10) for receiving a material and pretreating the material to form an intermediate product by acid treatment or alkali treatment, the material being fresh animal skin and the intermediate product comprising gelatin; a reaction part (20) disposed after the pretreatment part (10) for receiving the intermediate product and adding an enzyme to react with the intermediate product, thereby producing a collagen tripeptide (Gly-XY), the enzyme being a protease;and a refining section (30) disposed after the reaction section (20) to receive and refine the collagen tripeptide (Gly-XY) in order to form the collagen tripeptide microparticles (Gly-XY), the pretreatment section (10), the reaction section (20) and the refining section (30) being able to be connected together by conduits.;

2. System according to claim 1, characterized in that the material is fresh fish skin and the intermediate product is fish skin gelatin.

3. A system according to any one of claims 1 or 2, characterized in that the pretreatment part (10) comprises: a grinding unit (11) for receiving and grinding fresh animal hide to form a ground product; a screening unit (12) disposed after the grinding unit (11) for receiving and screening the ground product; a reaction processing unit (13) disposed after the screening unit (12) for receiving the screened and ground product and adding an acid or a base for the reaction to obtain a decomposed mixture; and an extraction unit (14) disposed after the reaction processing unit (13) for receiving and extracting the decomposed mixture to obtain the intermediate product.

4. System according to claim 1, characterized in that the reaction part (20) has a stirring unit (21), the stirring unit (21) comprising: a reservoir for receiving the enzyme and the intermediate product; and a stirring part disposed in the reservoir.

5. System according to claim 1, characterized in that the part of refining (30) includes: a concentration and sterilization unit (31) for performing a concentration and sterilization procedure on collagen tripeptide (Gly-XY); a drying unit (32) disposed after the concentration and sterilization unit (31) to heat and dry the concentrated and sterilized collagen tripeptide (Gly-XY) in order to produce a collagen tripeptide (Gly-XY) powder; and a coating unit (33) disposed after the drying unit to receive the collagen tripeptide powder (Gly-XY), and to mix and process the collagen tripeptide powder (Gly-XY) with a base material to form the collagen tripeptide microparticles (Gly-XY).

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