Analysis device

Abstract

To provide a plate for reaction to be used in an allergy test, aiming at higher sensitivity of the test and shorter testing time, capable of conducting the test easily through reducing a required amount of blood, etc. to serve as an analyte and reducing the number of processes of the test, while lowering an infection risk of a tester in the allergy test.SOLUTION: A plate for reaction is to be installed in a biochemical reaction substrate. In the plate for reaction, a flow-out prevention wall is formed to surround a reaction area in a manner of connected with a flow channel part.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] The present invention relates to a biochemical reaction substrate for measuring the amount of a sample substance having physiological activity based on a biochemical reaction process such as an immunological assay, and an analytical device for analyzing the reaction between a specimen such as blood and a reagent, and in particular to an analytical device that can achieve miniaturization of the analytical device, shortening of the testing time, reduction of the amount of specimen, and reduction of the testing cost. [Background technology]

[0002] Tests of biological substances, which check the presence and amount of substances in the body, are important for understanding health conditions and determining treatment methods. In addition, in today's world where living environments are becoming more and more diverse, it is necessary to perform many different types of tests, and there is a demand for faster and more sensitive testing of biological substances. For example, when treating allergic diseases, it is important to first understand the allergic disease that one is suffering from. In recent years, there have been many different causes of allergies, and it is necessary to identify the cause in order to receive appropriate treatment, such as using appropriate drugs.

[0003] Various methods are known as such allergy testing methods, and for example, as described in Patent Document 1, a method of quantifying IgE antibodies against specific allergens in a blood sample collected from a subject by solid-phase sandwich immunoassay is common. In this method, for example, a ligand capture antibody is adsorbed on a solid-phase carrier such as a glass filter, and protein adsorption sites other than the ligand capture antibody adsorption site are blocked with a blocking agent such as casein, and a ligand is bound to a specific allergen such as dust mites or pollen, which is mixed with a blood sample to form a complex between the specific allergen bound to the ligand and the IgE antibody against the specific allergen in the blood sample. Then, a mixture containing this complex is added to the above-mentioned solid-phase carrier on which the ligand capture antibody is adsorbed, and the ligand portion in the complex is bound to the ligand capture antibody, and then an anti-IgE antibody labeled with an enzyme or the like is added, and the IgE antibody portion in the complex is bound to the labeled anti-IgE antibody. Next, excess labeled anti-IgE antibody that has not bound to the complex is removed, and a color reaction according to the type of label is carried out to detect the labeled anti-IgE antibody bound to the IgE antibody. The detection results obtained are compared with a calibration curve prepared in advance using a standard IgE antibody, and the IgE antibody against the specific allergen in the blood sample is quantified.

[0004] To carry out the above test, a method and device for testing using a bioreaction substrate in which a specific allergen is bound to a porous filter of a bioreaction substrate is known. In addition, for the bioreaction substrate, a reaction vessel for immunoassays can be used in which glass fiber having appropriate physical strength is used at the bottom of a porous filter (solid phase carrier) and an absorption layer made of cellulose is combined at the bottom of the glass fiber to absorb the solution that has passed through the solid phase carrier (see Patent Document 2).

[0005] Similarly, as described in Patent Document 3, a biochip analysis method is disclosed that uses a biochip carrying antigens of various allergens as independent, i.e., spaced, spots, and that automates the process of detecting the reaction between the sample and the antigens after collection of the sample, and can rapidly obtain measurement results.

[0006] Similarly, as described in Patent Document 4, a method is disclosed in which a biochip carrying antigens of multiple allergens as independent or spaced apart spots is used to remove cleaning fluids, etc., without using a suction nozzle.

[0007] Furthermore, conventional analyzers for analyzing the reaction between a specimen such as blood and a reagent have been available in various forms. are known, and for example, an analytical device as shown in Patent Document 5 is known. The analytical device described in Patent Document 5 includes one or more test cartridges including at least a sample cell for accommodating a sample, a reagent cell for accommodating a reagent, and a reaction cell in which the sample and the reagent are reacted, and the cells are linearly arranged, a device housing having an internal space for a predetermined set stage and an adjacent test stage, a cartridge holding means provided on the set stage and having a cartridge receiving portion for holding the one or more test cartridges, a cartridge transport means provided on the test stage and configured to linearly carry the test cartridge held by the cartridge holding means into the test stage and transport the test cartridge along a longitudinal direction along the arrangement direction of each cell of the test cartridge in the carried-in test stage, while linearly transporting the test cartridge after the test from the test stage to the set stage and returning it to the cartridge receiving portion of the cartridge holding means, and a cartridge transport means provided in a portion of a transport path of the test cartridge in the test stage corresponding to a predetermined dispensing position, and configured to transport the test cartridge carried by the cartridge transport means. a specimen and reagent dispensing means for dispensing a specimen and a reagent of the specimen cartridge into a reaction cell of the specimen cartridge in a state where a dispensing target cell of the specimen cartridge in the inspection stage transported by the cartridge transporting means is transported to the measurement position and placed therein; a measuring means which is provided in a part of a transport path of the specimen cartridge in the inspection stage corresponding to a predetermined measurement position, and which measures a reaction between a specimen and a reagent in a reaction cell dispensed by the specimen and reagent dispensing means in a state where a reaction cell of the specimen cartridge in the inspection stage transported by the cartridge transporting means is transported to the measurement position and placed therein; a constant temperature bath which is heated by a heat source and keeps the liquid temperature in at least the reaction cell of the specimen cartridge in the inspection stage transported by the cartridge transporting means at a predetermined constant environmental temperature; and a constant temperature bath control means which has a temperature detector capable of detecting an internal environmental temperature of the inspection stage and controls a set temperature of the heat source of the constant temperature bath based on the internal environmental temperature detected by the temperature detector so that when the internal environmental temperature is lower than a predetermined threshold value, the set temperature of the heat source of the constant temperature bath is higher than when the temperature is equal to or higher than the threshold value.

[0008] According to such an analytical device, after the sample or reagent is dispensed into the reaction cell of the test cartridge, a constant temperature bath is provided which maintains the liquid temperature in the reaction cell of the test cartridge at a preset constant environmental temperature, thereby effectively preventing a decrease in measurement accuracy due to changes in the test cartridge and environmental temperature. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] JP 2011-133285 A [Patent Document 2] JP 2001-235471 A [Patent Document 3] JP 2011-13000 A [Patent Document 4] International Publication No. 2016 / 163494 [Patent Document 5] JP 2016-24054 A Summary of the Invention [Problem to be solved by the invention]

[0010] However, as described in Patent Document 2, the testing method and device using the reaction vessel can measure in a relatively short time, 12 minutes for one sample and about 39 minutes for 90 samples, but a sample (blood, etc.) must be prepared for each specific allergen, and a large amount of sample is required. Therefore, it was sometimes difficult to secure the sample required for measurement in infants.

[0011] In addition, the technology described in Patent Document 3 requires a suction nozzle to suck up each liquid supplied from each nozzle, such as a cleaning nozzle, an antibody nozzle, and a reagent nozzle. This requires time to complete the suction of each liquid, and also causes deposits on the suction nozzle to adhere to the biochip. There was a risk of contamination.

[0012] Furthermore, in the technology described in Patent Document 4, an IgE antibody against a specific allergen that specifically binds to antigens of various allergens immobilized on the second base is bound to an anti-IgE antibody labeled with an enzyme or the like (labeled anti-IgE antibody), and then a color reaction is carried out to detect the labeled anti-IgE antibody. However, because the first base and cover member are layered on top of the second base, this is not an effective means for detecting reactions in which the degree of coloration is weak, and there is a risk that detection cannot be performed with high sensitivity.

[0013] Thus, in conventional allergy tests, there is a demand for high sensitivity and short test times, and a demand for a small sample volume because a large amount of sample blood etc. is required. In addition, in allergy tests, there is a demand for a small number of reaction steps, which saves the labor of the tester, and there is a demand for a reduction in the risk of infection with a disease that the patient suffers from because the sample, blood etc., of the patient is used.

[0014] In addition, the analytical device described in Patent Document 5 dispenses a sample or reagent into a reaction cell, then aspirates and holds a predetermined amount of the sample or reagent, ejects a predetermined amount of the sample or reagent into the reaction cell to be measured, and then transports a test cartridge containing the reaction cell to a measurement position to perform the test.However, the method of aspirating the sample or reagent has the problem that the device becomes large and the cost of manufacturing the device increases.

[0015] Furthermore, in order to shorten the testing time, a method is known in which a specimen or reagent is dispensed into a reaction cell, the reaction cell is stirred, and then excess specimen and reagent are drained. However, providing such a stirring and draining mechanism results in problems such as an increase in the size of the analyzer and an increase in manufacturing costs.

[0016] The present invention has been made to solve these problems, and aims to provide a biochemical reaction substrate for use in allergy testing that increases the sensitivity of the test and shortens the test time, reduces the required amount of sample blood or the like, reduces the number of test steps, and makes it easier to perform the test, while reducing the risk of infection for the tester.

[0017] The present invention has been made to solve such problems, and aims to provide an analytical device that can be made smaller and whose manufacturing costs can be reduced even when a mechanism for stirring and draining the reaction cell is added to shorten the testing time. [Means for solving the problem]

[0018] The reaction plate of the present invention, which solves the above problems, is a reaction plate to be incorporated into a biochemical reaction substrate, and is characterized in that the reaction plate has an outflow prevention wall formed to surround the reaction area and continuous with the flow path section. Moreover, one embodiment of the invention is an analytical device for analyzing a reaction between a sample and a reagent, comprising an installation area for installing a reagent cartridge and a biochemical reaction substrate on a moving table, a dispensing area, a mixing area, a drainage area and a detection area, the installation area, the dispensing area, the mixing area, the drainage area and the detection area are arranged on the same straight line, the drainage area is located at either end of the same straight line, and the moving table is mounted so as to be able to move the installation area, the dispensing area, the mixing area, the drainage area and the detection area on a straight line. Moreover, one embodiment of the invention is an analytical device for analyzing a reaction between a sample and a reagent, comprising an installation area for installing a reagent cartridge and a biochemical reaction substrate, a dispensing area, a mixing area, a drainage area, and a detection area, wherein the installation area, the dispensing area, the mixing area, the drainage area, and the detection area are arranged in a collinear line.

[0019] Moreover, one embodiment of the invention is an analytical device for analyzing a reaction between a sample and a reagent, and preferably includes a mounting area for mounting a reagent cartridge and a biochemical reaction substrate on a moving table, a dispensing area, a mixing area, a drainage area, and a detection area, wherein the mounting area, dispensing area, mixing area, drainage area, and detection area are arranged on a same straight line, the drainage area includes a stopper formed on the same straight line and a tilting mechanism for tilting the biochemical reaction substrate by abutting against the stopper, a guide means arranged along the same straight line, and the moving table guided by the guide means. Moreover, one embodiment of the invention is an analytical device for analyzing a reaction between a specimen and a reagent, comprising: an installation area for installing a reagent cartridge containing the specimen and the reagent and a biochemical reaction substrate for dispensing the specimen and the reagent on a moving table; a dispensing area for dispensing the specimen and / or the reagent to the biochemical reaction substrate; a stirring area for stirring and mixing the dispensed specimen and / or the reagent; a drainage area for draining the stirred and mixed specimen and reagent; and a detection area for detecting the reaction between the specimen and the reagent in the biochemical reaction substrate, wherein the installation area, dispensing area, stirring area, drainage area and detection area are arranged on a same straight line, the drainage area is located at either end of the same straight line, and the moving table is mounted so as to be able to move the installation area, dispensing area, stirring area, drainage area and detection area on a straight line. Moreover, one embodiment of the invention is an analytical device for analyzing a reaction between a specimen and a reagent, comprising an installation area for installing a reagent cartridge in which the specimen and the reagent are contained and a biochemical reaction substrate for dispensing the specimen and the reagent, a dispensing area for dispensing the specimen and / or the reagent to the biochemical reaction substrate, a stirring area for stirring and mixing the dispensed specimen and / or the reagent, a drainage area for draining the stirred and mixed specimen and reagent, and a detection area for detecting the reaction between the specimen and the reagent in the biochemical reaction substrate, wherein the installation area, the dispensing area, the stirring area, the drainage area and the detection area are arranged on the same straight line.

[0020] In one embodiment of the invention, the moving table preferably includes a substrate holder on which the reagent cartridge is placed and on which the biochemical reaction substrate is placed. In one embodiment of the invention, it is preferable that the installation area and the drainage area are each disposed at either end of the same straight line.

[0021] Moreover, in one embodiment of the invention, the biochemical reaction substrate includes a reaction plate having a reaction area on which a specific binding substance that specifically reacts with a test substance in a specimen is immobilized, and the tilting mechanism preferably drains excess of the specimen and the reagent from the reaction area of ​​the biochemical reaction substrate by tilting the substrate holding portion. In one embodiment of the invention, it is preferable that the drainage area includes a tilting mechanism for tilting the biochemical reaction substrate.

[0022] In one embodiment of the invention, it is preferable to include a guide means arranged along the same straight line, and the moving table guided by the guide means.

[0023] In one embodiment of the invention, it is preferable that, in the stirring area, the biochemical reaction substrate mounted on the moving table is vibrated by reciprocating along the same straight line.

[0024] Moreover, in one embodiment of the invention, it is preferable that, in the stirring area, the biochemical reaction substrate mounted on the moving table moves back and forth within the stirring area along the guiding means, thereby stirring the dispensed specimen and reagent.

[0025] In one embodiment of the invention, it is preferable that the dispensing area is provided with a dispensing nozzle that is movable in a direction approximately perpendicular to the same straight line.

[0026] Moreover, in one embodiment of the invention, the biochemical reaction substrate preferably comprises a reaction plate, an absorbent, a reaction plate storage section for storing the reaction plate, and an absorbent storage section for storing the absorbent, and the reaction plate preferably has a reaction area in which a specific binding substance that specifically reacts with a test substance in a specimen is immobilized, and a flow path section connecting the absorbent and the reaction area.

[0027] Moreover, in one embodiment of the invention, it is preferable to include a guide means arranged along the same straight line and a moving table guided by the guide means, and the moving table includes a substrate holding section on which the reagent cartridge is placed and on which the biochemical reaction substrate is placed.

[0028] In one embodiment of the invention, it is preferable that the tilting mechanism drains excess sample and reagent from the reaction area of ​​the biochemical reaction substrate into an absorbent storage section by tilting the substrate holding section.

[0029] In one embodiment of the invention, the tilting mechanism preferably causes the excess sample and reagent to be absorbed by the absorbent housed in the absorbent housing section and then drained.

[0030] Moreover, in one embodiment of the invention, the detection area preferably includes a light-shielding section that shields the biochemical reaction substrate from light, and a detection camera that detects the surface of the biochemical reaction substrate, and the detection camera preferably includes a moving mechanism that moves the detection camera in a direction approximately perpendicular to the same straight line.

[0031] In addition, in one embodiment of the invention, it is preferable that the light blocking portion is moved together with the detection camera by the movement mechanism and includes an adjustment mechanism capable of adjusting the distance from the detection camera to an end of the light blocking portion.

[0032] In one embodiment of the invention, the adjustment mechanism preferably includes a first cylinder having the detection camera attached to one end thereof, and a second cylinder assembled to the first cylinder.

[0033] In one embodiment of the invention, it is preferable that, after drainage of the specimen and the reagent is completed, the moving mechanism lowers the detection camera and brings the light-shielding part into close contact with the biochemical reaction substrate. Effect of the Invention

[0034] Furthermore, according to the present invention, the installation area, dispensing area, mixing area, drainage area and detection area are arranged in the same straight line, so that the analytical device can be made smaller even when the chip device into which the sample and reagent have been dispensed is stirred or drained to shorten the testing time. [Brief description of the drawings]

[0035] [Figure 1] FIG. 1 is a perspective view of a biochemical reaction substrate according to an embodiment of the present invention. [Diagram 2] FIG. 2 is an exploded view of a biochemical reaction substrate according to an embodiment of the present invention. [Diagram 3] FIG. 2 is a perspective view of a reaction plate used in a biochemical reaction substrate according to an embodiment of the present invention. [Figure 4] FIG. 2 is a top view of a storage container used in the biochemical reaction substrate according to the embodiment of the present invention. [Diagram 5]FIG. 2 is a bottom view of a cover used in the biochemical reaction substrate according to the embodiment of the present invention. [Figure 6] FIG. 2 is a top view for explaining the arrangement of antigens (allergens) in the reaction area of ​​the reaction plate. [Figure 7] FIG. 13 is a bottom view showing a modified example of the cover used in the biochemical reaction substrate according to the embodiment of the present invention. [Figure 8] FIG. 13 is a top view showing a modified example of a reaction plate used in a biochemical reaction substrate according to an embodiment of the present invention. [Figure 9] AA cross-sectional view in FIG. 8. [Figure 10] BB cross-sectional view in FIG. 8. [Figure 11] FIG. 13 is a top view for explaining another arrangement state of antigens (allergens) in the reaction area of ​​the reaction plate. [Figure 12] FIG. 1 is a perspective view of an analysis device according to an embodiment of the present invention. [Figure 13] FIG. 2 is a diagram for explaining the internal structure of the analysis device according to the embodiment of the present invention. [Figure 14] FIG. 2 is a perspective view illustrating the internal structure of the analysis device according to the embodiment of the present invention. [Figure 15] FIG. 2 is a perspective view illustrating an installation area of ​​the analysis device according to the embodiment of the present invention. [Figure 16] FIG. 2 is a perspective view illustrating a barcode reading area of ​​the analysis device according to the embodiment of the present invention. [Figure 17] FIG. 2 is a perspective view illustrating a dispensing area of ​​the analyzer according to the embodiment of the present invention. [Figure 18] FIG. 2 is a perspective view illustrating a stirring area of ​​the analyzer according to the embodiment of the present invention. [Figure 19] FIG. 2 is a perspective view illustrating a drainage area of ​​the analyzer according to the embodiment of the present invention. [Figure 20] FIG. 4 is a perspective view illustrating a tilt mechanism of the analysis device according to the embodiment of the present invention. [Figure 21] FIG. 13 is a perspective view for explaining a tilting mechanism of the analyzer according to the embodiment of the present invention, showing a state in which the tilting cam abuts against a stopper. [Figure 22] FIG. 2 is a perspective view for explaining a tilting mechanism of the analysis device according to the embodiment of the present invention, showing a state in which the substrate holding part is tilted. [Figure 23] FIG. 2 is a perspective view illustrating a detection area of ​​the analysis device according to the embodiment of the present invention. [Figure 24] FIG. 4 is a diagram for explaining the detection result of the analysis device according to the embodiment of the present invention, and is a diagram for explaining the arrangement of antigens in a reaction area. [Diagram 25] FIG. 13 is a diagram for explaining the detection result of the analysis device according to the embodiment of the present invention, showing a state after exposure for 60 seconds. [Figure 26] FIG. 13 is a diagram for explaining the detection result of the analysis device according to the embodiment of the present invention, showing a state after exposure for 20 seconds. [Figure 27] FIG. 13 is a diagram for explaining the detection result of the analysis device according to the embodiment of the present invention, showing a state where exposure is performed for 5 seconds. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0036] The biochemical reaction substrate and the analytical device according to the present invention will be described below with reference to the drawings. Note that the following embodiments do not limit the invention according to each claim, and all of the combinations of features described in the embodiments are not necessarily essential to the solution of the invention.

[0037] 1 is a perspective view of a biochemical reaction substrate according to an embodiment of the present invention, FIG. 2 is an exploded view of the biochemical reaction substrate according to an embodiment of the present invention, FIG. 3 is a perspective view of a reaction plate used in the biochemical reaction substrate according to an embodiment of the present invention, FIG. 4 is a top view of a storage container used in the biochemical reaction substrate according to an embodiment of the present invention, FIG. 5 is a bottom view of a cover used in the biochemical reaction substrate according to an embodiment of the present invention, FIG. 6 is a top view for explaining the arrangement of antigens (allergens) in the reaction area of ​​the reaction plate, FIG. 7 is a bottom view showing a modified cover used in the biochemical reaction substrate according to an embodiment of the present invention, FIG. 8 is a top view showing a modified reaction plate used in the biochemical reaction substrate according to an embodiment of the present invention, FIG. 9 is an AA cross-sectional view in FIG. 8, FIG. 10 is a BB cross-sectional view in FIG. 8, and FIG. 11 is a diagram showing the arrangement of antigens (allergens) in the reaction area of ​​the reaction plate. FIG. 12 is a top view illustrating another arrangement state of the analyzing device according to an embodiment of the present invention, FIG. 13 is a diagram illustrating the internal structure of the analyzing device according to an embodiment of the present invention, FIG. 14 is a perspective view illustrating the internal structure of the analyzing device according to an embodiment of the present invention, FIG. 15 is a perspective view illustrating the installation area of ​​the analyzing device according to an embodiment of the present invention, FIG. 16 is a perspective view illustrating the barcode reading area of ​​the analyzing device according to an embodiment of the present invention, FIG. 17 is a perspective view illustrating the dispensing area of ​​the analyzing device according to an embodiment of the invention, FIG. 18 is a perspective view illustrating the stirring area of ​​the analyzing device according to an embodiment of the invention, FIG. 19 is a perspective view illustrating the drainage area of ​​the analyzing device according to an embodiment of the present invention, and FIG. Fig. 21 is a perspective view for explaining the tilt mechanism of the analyzer according to the embodiment of the present invention, showing a state in which the tilt cam is in contact with the stopper, Fig. 22 is a perspective view for explaining the tilt mechanism of the analyzer according to the embodiment of the present invention, showing a state in which the substrate holder is inclined, Fig. 23 is a perspective view for explaining the detection area of ​​the analyzer according to the embodiment of the present invention, Fig. 24 is a view for explaining the detection result of the analyzer according to the embodiment of the present invention, showing the arrangement of antigens in the reaction area, Fig. 25 is a view for explaining the detection result of the analyzer according to the embodiment of the present invention, showing a state after exposure for 60 seconds, Fig. 26 is a view for explaining the detection result of the analyzer according to the embodiment of the present invention, showing a state after exposure for 20 seconds, and Fig. 27 is a view for explaining the detection result of the analyzer according to the embodiment of the present invention, showing a state after exposure for 5 seconds.

[0038] 1 and 2, the biochemical reaction substrate 1 according to this embodiment is capable of housing a reaction plate 10 therein, and has a cover 30 and a storage container 40. A substantially circular injection hole 31 is formed in the cover 30, and the injection hole 31 is configured to be disposed at a position corresponding to the reaction area 11 of the reaction plate 10 when the reaction plate 10 is housed therein. In addition, a concave heat-retaining portion 43 is formed in the bottom of the storage container 40, and is configured so that a heater of an inspection device described later can come into contact with the heat to heat the reaction area 11.

[0039] 2, the storage container 40 is formed with a reaction plate storage section 41 for storing the reaction plate 10 and an absorber storage section 42 for storing the absorber 20 made of a porous material such as a sponge that can sufficiently absorb liquid. Note that, in a state where the reaction plate 10 and the absorber 20 are stored in the storage container 40, the tip of the flow path section 12 formed in the reaction plate 10 is disposed so as to abut against the absorber 20.

[0040] 3, the reaction plate 10 includes a plate-shaped base portion 14 that can be fitted into a reaction plate storage portion 41 formed in a storage container 40 without any trouble, an outflow prevention wall 13 standing upright from the base portion 14, and a flow path portion 12. The outflow prevention wall 13 is a roughly annular wall, and the inside of the outflow prevention wall 13 is defined as a reaction area 11.

[0041] In addition, at a position facing the absorbent 20 from the reaction area 11, a flow path section 12 is formed, which extends from the outer periphery of the outflow prevention wall 13 and is formed so that the distance between a pair of side walls gradually narrows. The flow path section 12 is formed to drain the specimen dispensed in the reaction area 11 and absorb it in the absorbent 20, and has a slope 15 formed to rise from the reaction area 11 so that the specimen can be discharged from the reaction area 11 toward the absorbent 20 without waste by tilting the biochemical reaction substrate 1. The slope 15 is preferably formed to be inclined at about 5° to 70° from the base section 14, and the more preferable angle of the slope is 12° to 50°, the further preferable angle is 17° to 35°, and the suitable angle is 23°. The greater the angle of the slope of the flow path section, the more leakage can be prevented, but on the other hand, it is more likely that a situation in which it is difficult to discharge occurs. Therefore, it is preferable to set the preferable angle By adopting this, the object of the present invention can be achieved. The base end of the flow channel section 12 on the reaction area 11 side is configured to have an opening as wide as possible in order to smoothly guide the specimen, etc. on the reaction area 11 to the tip on the absorber 20 side, and the tip side of the flow channel section 12 is formed so that the inclined surface 15 abuts against the absorber 20 at an acute angle.

[0042] In this way, the reaction plate 10 used in the biochemical reaction substrate 1 according to this embodiment has the outflow prevention wall 13 formed so as to surround the reaction area 11, and the flow path section 12 is provided with the inclined surface 15, so that in an allergy test, even when the biochemical reaction substrate 1 is stirred, the specimen, etc. is prevented from flowing out from the reaction area 11, and even when the specimen, etc. is drained after stirring, the specimen, etc. can be easily discharged from the flow path section 12. In addition, by applying a blocking agent to the entire reaction area in advance, nonspecific adsorption can be suppressed. Furthermore, by defining the inside of the outflow prevention wall 13 as the reaction area, it is also possible to more easily discharge the specimen, etc., as a secondary effect of applying the blocking agent.

[0043] As a blocking agent, synthetic polymers that are not derived from animals or plants, such as polyethylene glycol, can be used using common techniques, and can be appropriately selected depending on the material of the reaction plate 11, the target substance such as an antigen, the specimen such as blood, and the properties of reagents such as cleaning solutions.

[0044] As shown in Fig. 4, the storage container 40 may be formed with a reaction plate storage section 41 and an absorber storage section 42. The storage container 40 is a bottomed box-shaped member having an open end at the top, and when a rib 45 is formed on the inner wall of the absorber storage section 42, it becomes possible to hold the absorber 20 more reliably. It is preferable that the reaction plate storage section 41 has an opening 46 formed at a position corresponding to the reaction area 11 when the reaction plate 10 is placed thereon. The storage container 40 can heat the reaction area 11 directly from outside the biochemical reaction substrate 1 through the opening 46.

[0045] Furthermore, the reaction plate storage section 41 and the absorbent storage section 42 are connected to each other by a storage section groove 44, so that even if a specimen or the like leaks beyond the outflow prevention wall 13 due to stirring or the like, the specimen or the like can be guided from the reaction plate storage section 41 to the absorbent storage section 42.

[0046] 5, the cover 30 is a member that closes the open end of the storage container 40 described above and constitutes the outer shell of the biochemical reaction base 1, and has a side wall portion 36a that extends downward from the outer periphery of the top surface 36. An inner wall portion 32 is formed to hang down from the top surface 36 at a position corresponding to the outflow prevention wall 13 of the reaction plate 10, and when the reaction plate 10 is stored in the biochemical reaction base 1, the inner wall portion 32 abuts against the outflow prevention wall 13.

[0047] In addition, a partition wall 37 is formed extending downward from the top surface 36 at a position corresponding to the continuous portion of the reaction plate storage section 41 and the absorbent storage section 42 of the storage container 40. This partition wall 37 separates the reaction area 11 of the reaction plate 10 from the absorbent 20. The inner wall section 32 and the partition wall 37 are formed discontinuously with a cutout section 33 interposed therebetween. If the inner wall section 32 is extended up to the cutout section 33, a minute gap is generated between the outer periphery of the flow path section 12 and the inner wall section 32, and the presence of this gap may cause leakage to the outside as viewed from the reaction area of ​​the outflow prevention wall 13 due to capillary action, or the specimen or the like absorbed by the absorbent 20 may flow back to the reaction area 11 side. This cutout section 33 is provided to prevent this.

[0048] Furthermore, the inner wall portion 32 may have an adjustment cutout portion 34 formed at an end portion in the longitudinal direction of the cover 30. By forming a part of the inner wall portion 32 as the inner wall of the side wall portion 36a, it is possible to reduce space and material. This will enable cost reductions such as expenses.

[0049] Furthermore, uneven anti-slip means 35 is formed on the outer circumferential side wall of the cover 30, and the anti-slip means 35 is configured to enable an examiner to securely hold the biochemical reaction substrate 1. Furthermore, ribs 38 may be formed on the inner wall of the side wall portion 36a corresponding to the absorbent housing portion 42, similar to the absorbent housing portion 42.

[0050] As shown in FIG. 6, in the reaction area 11 of the reaction plate 10, a plurality of sets (e.g., 3 spots / 1 set) of antigens 50 are arranged in a predetermined number of sets (e.g., 156 spots / 52 sets). By arranging them in this way, the amount of antigens 50 placed in the reaction area 11 can be increased, and it becomes possible to detect a plurality of allergic reactions in one test. This can be realized by making the type of antigen the same for each set and eliminating the influence during measurement by placing a distance between the sets. In addition, the distance between the sets is preferably 0.5 mm or more, more preferably 0.5 mm to 2.0 mm, even more preferably 0.8 mm to 1.6 mm, and preferably about 1.2 mm. This allows, for example, a test of IgE antibodies in a blood sample against different types of antigens (allergens) to be performed at one time. Therefore, the test can be performed with a small amount of sample, in a short time, and with a reduced number of steps.

[0051] 7, like the shape of the cutout in cover 30a, a cutout wall 37a formed in a substantially L-shape so that inner wall 32 and cutout wall 37 are continuous may be formed, and an inner wall cutout 33a and a cutout wall cutout 33b may be formed in inner wall 32 and cutout wall 37a, inner wall 32 and side wall 36a may be formed discontinuously. By forming inner wall cutout 33a and cutout wall cutout 33b in this way, there is a risk of leakage to the outside as viewed from the reaction area of ​​outflow prevention wall 13 due to capillary action, or a risk of the specimen or the like absorbed by absorber 20 flowing back to the reaction area 11, and this is prevented.

[0052] As shown in Figures 8 to 10, it is preferable to form the inclined surface 15a formed in the flow path section 12a of the reaction plate 10a so that the specimen, etc. can be discharged more easily. As shown in Figure 9, the inclined surface 15a is formed so that the continuous portion with the reaction area 11 is smoothly formed so that the specimen, etc. can be discharged without waste, and the specimen, etc. is prevented from accumulating in the continuous portion between the reaction area 11 and the inclined surface 15a. Furthermore, as shown in Figure 10, the cross-sectional shape of the inclined surface 15a itself is also configured to be a smooth curve, and the specimen, etc. is prevented from accumulating on the inclined surface 15a.

[0053] Furthermore, it is preferable that the antigens 50 arranged in the reaction area 11 are arranged so as to be concentrated in the center of the reaction plate 10, as shown in Fig. 11. By adjusting the arrangement location of the antigens 50 in this manner, it is possible to shorten the time required to immobilize the antigens 50 on the reaction plate 10.

[0054] Next, the operation of an analyzer for performing an allergy test using the biochemical reaction substrate 1 according to this embodiment will be described with reference to FIGS.

[0055] 12, an analyzer 101 according to this embodiment includes a housing 104 having an operation panel 102 and an input section 103 for mounting a reagent cartridge 113 (described later) and a biochemical reaction substrate 1. The input section 103 can be opened and closed by a mounting door (not shown).

[0056] As shown in Fig. 13, the inside of the analyzer 101 is divided into an installation area 110, a barcode reading area 120, a dispensing area 130, a mixing area 140, a detection area 160, and a drainage area 150, which are arranged on the same straight line. A movable table 116 is movably attached to the rear 150 and is guided by a guiding means 117 arranged on the same straight line. In addition, for example, the mixing area 140 and the dispensing area 130 may have overlapping portions, and adjacent areas may be configured to overlap each other.

[0057] 14, the moving table 116 is provided with a substrate holding section 116a on which the reagent cartridge 113 is placed and on which the biochemical reaction substrate 1 is placed. Also, a driving section 118 is attached, which extends substantially parallel to the guide means 117. A circular band rotated by a drive source such as a motor (not shown) is stretched around the driving section 118, and the driving force of the band is transmitted to the moving table 116, thereby making it possible for the moving table 116 to move along the guide means 117.

[0058] Next, each of the installation area 110, the barcode reading area 120, the dispensing area 130, the mixing area 140, the detection area 160 and the drainage area 150 will be described.

[0059] As shown in Figures 13 and 15, the installation area 110 is located at one end side of the guide means 117 (the left end in Figure 13). Also, as shown in Figure 15, when the moving table 116 is located in the installation area 110, the reagent cartridge 113 and the biochemical reaction substrate 1 can be installed on the moving table 116 from outside the housing 104 via the input unit 103. The reagent cartridge 113 is configured to be able to store the specimen 111, the reaction reagent 112a, the washing liquid 112b, and the tip 132. In the following description of the present application, the reaction reagent 112a and the washing liquid 112b are collectively referred to as the "reagent 112". The biochemical reaction substrate 1 includes an injection hole 31 that opens toward the reaction area 11 into which the specimen 111 and the reagent 112 are dispensed as described above, and an absorbent storage section 42 for draining excess specimen 111 and reagent 112 after dispensing the specimen 111 and the reagent 112 and stirring the biochemical reaction substrate 1. The absorbent storage section 42 contains an absorbent (not shown), and the absorbent holds the excess specimen 111 and the reagent 112 so that they do not flow out to the outside. The reaction reagent is a reagent for a reaction required for detection, and is, for example, a labeled antibody or a luminescent substrate. The reaction reagent may be any reagent required for the reaction, and is not limited to these specific examples. The positions of the reaction reagent 112a and the cleaning solution 112b can be appropriately determined, and are not limited to the positions shown in FIG. 15.

[0060] In addition, a heating section 115 is attached to the substrate holding section 116a on which the biochemical reaction substrate 1 is mounted so as to correspond to the reaction area 11 of the biochemical reaction substrate 1, and the reaction area is heated to body temperature (e.g., about 37°C) by the heating section 115, thereby shortening the reaction time.

[0061] 16, the moving table 116 is moved along the guide means 117 to the barcode reading area 120. A barcode reader 121 is attached to the barcode reading area 120, and the barcodes printed or affixed to the reagent cartridge 113 and the side wall of the biochemical reaction substrate 1 are read by the barcode reader 121, thereby allowing the analyzer 101 to read reagent information such as the reagent, the expiration date and lot of the reagent, and calibration curve information.

[0062] As shown in Fig. 17, the dispensing area 130 is equipped with a dispensing nozzle 131 attached so as to be movable in a direction approximately perpendicular to the guide means 117. The dispensing nozzle 131 is a member that aspirates and discharges the specimen 111 and the reagent 112 contained in the reagent cartridge 113, and dispenses the specimen 111 and the reagent 112 to the biochemical reaction substrate 1. In this dispensing, first, in order to fit the tip 132 contained in the reagent cartridge 113 to the tip of the dispensing nozzle 131, the moving table 116 is moved so that the position of the tip 132 contained in the reagent cartridge 113 is aligned with the position of the dispensing nozzle 131. After being moved to a position directly below, the dispensing nozzle 131 is lowered so that the tip 132 fits onto the tip of the dispensing nozzle 131.

[0063] Next, in order to aspirate the specimen 111, reagent 112, etc. on the moving table 116 from the reagent cartridge 113, the moving table 116 is moved so that the position where the specimen 111 or reagent 112, etc. is stored on the moving table 116 is positioned directly below the dispensing nozzle 131, and the specimen 111 or reagent 112 is aspirated. Similarly, after aspirating the specimen 111 or reagent 112, the moving table 116 is moved so that the injection hole 31 of the biochemical reaction substrate 1 is positioned directly below the dispensing nozzle 131, and the aspirated specimen 111, reagent 112, etc. are dispensed into the reaction area 11 via the injection hole 31 of the biochemical reaction substrate 1.

[0064] The used tip 132 is separated from the dispensing nozzle 131 and returned to a predetermined position in the reagent cartridge 113. At this time, since the used tip 132 is returned to its original position, there is no need to provide a position for collecting the used tip 132, so that the size of the reagent cartridge 113 can be reduced and the used tip 132 can be reliably collected.

[0065] The stirring area 140 is located between the dispensing area 130 and the barcode reading area 120, and as shown in FIG. 18, the movable table 116 is moved back and forth within the stirring area 140 along the guide means 117 to appropriately stir the dispensed specimen 111 and reagent 112. At this time, the dispensing nozzle 131 and the detection camera 161 described later are configured to retreat upward so as not to interfere with the movable table 116 during stirring. The stirring speed may be any speed as long as the specimen 111 and the reagent 112 can be reliably stirred. For example, it is preferable to stir at a speed at which the movable table can be moved about 60 to 160 times with an amplitude of 1 cm per minute, more preferably at a stirring speed of about 100 to 160 times, even more preferably at a stirring speed of about 120 to 160 times, and optimally at a stirring speed of about 140 times.

[0066] Next, as shown in Fig. 19, the moving table 116 is moved to the drainage area 150, and the excess specimen 111 and reagent 112 are absorbed by the absorbent 20 stored in the absorbent storage section 42 of the biochemical reaction substrate 1, and drained. The drainage area 150 is disposed at the other end of the guide means 117, and as shown in Fig. 20, a stopper 152 is attached to the end of the guide means 117. The moving table 116 is also provided with a tilting mechanism that tilts the substrate holding section 116a at a predetermined angle when it reaches the drainage area 150. The tilting mechanism tilts the substrate holding section 116a, thereby enabling the specimen 111 and reagent 112 to be smoothly drained from the reaction area 11 of the biochemical reaction substrate 1 to the absorbent storage section 42. The inclination angle of the tilting mechanism may be any angle as long as it allows for smooth drainage. For example, the inclination angle can be in the range of from the same angle as the inclined surface 15 formed between the reaction area 11 and the absorber storage section 42 in the biochemical reaction substrate 1 to 90°. Preferably, the inclination angle is 23° or more, more preferably, 30° to 70°, even more preferably, 30° to 60°, and most preferably, 50°.

[0067] Next, the operation of the tilting mechanism will be described with reference to Figures 19 to 22. A tilting cam 151 is attached to the base holding portion 116a, and as shown in Figure 21, when the moving table 116 moves to the drainage area 150, the tilting cam 151 comes into contact with a stopper 152 attached to the end of the guide means 117. Then, as shown in Figure 22, when the moving table 116 is further moved toward the stopper 152, the tilting cam 151 rotates around the rotating shaft 155, and the roller 154 attached to the tip of the tilting cam 151 moves so as to be lifted upward. 19. As a result of this movement of roller 154, base body holding portion 116a is lifted with rotation shaft 153 as a fulcrum, as shown in FIG. 19. In this manner, roller 154 is attached to the tip of tilting cam 151, so that base body holding portion 116a can be smoothly tilted. After the liquid has been drained, moving table 116 is moved to detection area 160, which will be described later. At this time, the contact between stopper 152 and tilting cam 151 is released, so that the tilt of base body holding portion 116a is also released at the same time, and the upper surfaces of moving table 116 and base body holding portion 116a return to a substantially horizontal state.

[0068] Next, as shown in FIG. 23, the moving table 116 is moved to the detection area 160. The detection area 160 includes a detection camera 161, a moving mechanism 165 for moving the detection camera 161 up and down in a direction substantially perpendicular to the guide means 117, and a first cylinder 164 and a second cylinder 163 that move up and down together with the detection camera 161. A light-shielding part 162 is attached to the tip of the second cylinder 163, and when the detection camera 161 is moved to the lower end by the moving mechanism 165, the reaction area 11 can be covered so as to light-shield the injection hole 31 of the biochemical reaction substrate 1. The first cylinder 164 has the detection camera 161 attached to its upper end and is fitted with the second cylinder 163. The fitting parts of the first cylinder 164 and the second cylinder 163 are assembled to be light-shielding via an O-ring (not shown) or the like, and are held together by the elastic force of the O-ring. In order to adjust the distance between the reaction area 11 of the biochemical reaction substrate 1 and the detection camera 161 and to focus when the detection camera 161 is lowered, the first cylinder 164 and the second cylinder 163 are provided with an adjustment mechanism that allows them to move relative to each other, and this adjustment mechanism is configured by interposing the above-mentioned O-ring. In this way, since the adjustment mechanism is configured by attaching the first cylinder 164 and the second cylinder 163 to each other with the O-ring, the second cylinder 163 can slide up and down on the contact surface of the O-ring and move up and down relative to the first cylinder 164.

[0069] In this manner, after the discharge of the specimen or the like is completed in the detection area 160, as shown in Fig. 23, the detection camera 161 is lowered and the light-shielding part 162 provided at the tip of the camera is brought into close contact with the biochemical reaction substrate 1, blocking light from entering from outside the biochemical reaction substrate 1 and the analyzer 101. In this state, the presence or absence of luminescence of the labeled antibody after a predetermined reaction time has elapsed can be detected to detect the presence or absence of an allergic reaction. The luminescence of the labeled antibody is achieved by dispensing a luminescent base material that causes the labeled anti-IgE antibody to luminesce and become visible as a reagent (reaction reagent), and this luminescent base material is used to carry out a biochemical reaction in the reaction area for a predetermined time under predetermined environmental conditions, and the intensity of the luminescence is measured by the detection camera 161.

[0070] In this way, by configuring the dispensing nozzle 131 and the detection camera 161 to move up and down together with the second tube 163, the first tube 164, and the light-shielding part 162 and making it possible to move the position of the biochemical reaction substrate 1, it is possible to prevent the cover and the moving mechanism from becoming larger than in a mechanism that moves the light-shielding part and cover while keeping the detection camera 161 fixed, and this contributes to the overall miniaturization of the analysis device 101. Normally, it is desirable to fix the distance between the biochemical reaction substrate 1 on which the detection target is located and the detection camera 161 at a certain distance in order to perform measurement with high accuracy, but if such a fixed method is adopted, it is necessary to move up and down a hood that covers the detection camera and the biochemical reaction substrate 1 and constitutes a darkroom structure to block light during measurement, and this hood becomes larger and needs to cover the entire biochemical reaction substrate 1 to block light. In order to completely block light, a structure is required to attach an elastic body to the bottom of the hood so that it is in close contact with the base (non-movable part) on which the biochemical reaction substrate 1 is placed, etc., making it difficult to reduce the size of the analysis device 101. In contrast, the analysis device 101 according to this embodiment moves the detection camera 161 itself up and down, thereby reducing the space of the darkroom structure and making it possible to reduce the size of the moving mechanism. Furthermore, by disposing the second tube 163 inside the first tube 164 and attaching the light shielding part 162 to the tip of the second tube 163, the light shielding part 162 can be brought into close contact with the biochemical reaction base 1 to shield it from light. The light can be blocked in a space that is slightly larger or approximately equal to the area covered by the entire area, or a space that is small enough to cover the injection hole 31, and the moving mechanism can be made smaller. In addition, a moving mechanism for various directions is required in the analyzer, such as moving the dispensing nozzle 131, tilting the substrate holding part 116a, and moving the barcode reader 121 and the like in accordance with the biochemical reaction substrate 1, but providing a moving mechanism corresponding to each moving direction leads to an increase in the size of the analyzer. In contrast, the analyzer 101 according to this embodiment has the installation area 110, the dispensing area 130, the mixing area 140, the drainage area 150, and the detection area 160 arranged in the same straight line, and the moving mechanisms for the biochemical reaction substrate 1 that move between each area are integrated on one axis, thereby making the analyzer 101 smaller in size.

[0071] With this type of analytical device, it is possible to increase the sensitivity of allergy testing and shorten the testing time, while reducing the amount of sample blood and other materials required and reducing the number of testing steps, making it easier to perform the test and reducing the risk of infection for the tester.In addition, by arranging the installation area, dispensing area, mixing area, drainage area, and detection area on the same line, all steps can be performed with just movement on a single axis, making it possible to miniaturize the entire device. EXAMPLES

[0072] Next, the detection results of the analysis device 101 according to this embodiment will be described with reference to Figures 24 to 27. As shown in Figure 24, in this embodiment, antigens A (cockroach), B (Timothy), and C (salmon) were arranged in the reaction area of ​​the biochemical reaction substrate and tested.

[0073] As shown in Figure 25, after stirring and exposure for 60 seconds, antigen A and antigen B emitted light, and the light emission was confirmed by the detection camera. In addition, no strong light emission was confirmed for antigen C, and it was confirmed that this sample contained a trace amount of IgE antibody against antigen C.

[0074] It was also confirmed that the luminescence caused by residual drainage around the reaction area was suppressed. Although some luminescence was observed around the reaction area, it was also confirmed that this luminescence was within a range that did not affect the analysis and did not affect the detection performance of the analyzer.

[0075] For antigen B, the light emission is strong when the exposure time is set to 60 seconds. In contrast, the analysis device 101 according to this embodiment captures images with exposure times of 5 seconds, 20 seconds, and 60 seconds, as shown in Figs. 25 to 27. As a result, the light emission of antigen B is strong after 60 seconds has elapsed and affects the detection performance, but as shown in Fig. 26, when the exposure time is set to 20 seconds, the light emission of antigen B is in an appropriate intensity state, so that the detection performance is ensured for antigen B by using the detection result with an exposure time of 20 seconds. Furthermore, in the analysis device 101 according to this embodiment, an image is also captured with an exposure time of 5 seconds as shown in Fig. 27, but for antigens A to C this time, the light emission was weak at 5 seconds. However, for antigens with strong light emission, the detection performance of the analysis device 101 can be further improved by using the detection result with an exposure time of 5 seconds.

[0076] In the above-described embodiment of the biochemical reaction substrate 1, the injection hole 31 and the reaction area 11 are formed in a circular shape. However, the shapes of these are not limited to a circular shape and may be, for example, an elliptical shape.

[0077] In addition, the reaction plate 10 used in the biochemical reaction substrate 1 according to the present embodiment described above can be a transparent or colored plate, but using a black plate allows for more sensitive measurement. By providing the reaction area 11 including the inside of the leakage prevention wall 13, the inclined surface 15, and the inside of the flow channel section 12, non-specific adsorption can be further suppressed.

[0078] Also, the tilt direction by the tilt mechanism used in the analyzer 101 according to this embodiment has been explained using Fig. 19, but is not limited to tilting in a direction perpendicular to the guide means 117, and may be tilted, for example, horizontally with respect to the guide means 117 depending on the position of the tilt cam 151 or the position of the rotation shaft 153. Furthermore, the reagent cartridge 113 may be one in which all reagents and samples used in the measurement are combined into one, or two or more reagent cartridges may be used depending on the application, etc. It is clear from the claims that such modified or improved forms are also included within the technical scope of the present invention. [Explanation of symbols]

[0079] 1 Biochemical reaction substrate, 10 Reaction plate, 11 Reaction area, 12 Flow path, 13, spill prevention wall, 14, base, 15, inclined surface, 20, absorber, 30, cover, 31, injection hole, 32, inner wall, 33, suction prevention recess, 34, adjustment recess, 35, slip prevention means, 36, top surface, 37 Partition wall, 38, 45 rib, 40 storage container, 41 reaction plate storage section, 42 absorbent storage section, 43 heat retention section, 44 storage section groove, 46 opening, 50 antigen, 51 set, 101 analyzer, 102 operation section, 103 input section, 104 housing, 110 installation area, 111 sample, 112a reaction reagent, 112b cleaning solution, 113 reagent cartridge, 115 heating section, 116 moving table, 116a substrate holding section, 117 guiding means, 118 driving section, 120 barcode reading section, 121 barcode reader, 130 dispensing area, 131 dispensing nozzle, 132 tip, 140 mixing area, 150 drainage area, 151 tilting cam, 152 stopper, 153 rotating shaft, 154 roller, 155 rotating shaft, 160 detection area, 161 detection camera, 162 light shielding section, 163 second tube, 164 first tube, 165 moving mechanism.

Claims

1. An analytical device for analyzing a reaction between a specimen and a reagent, comprising: The apparatus includes a mounting area for mounting the reagent cartridge and the biochemical reaction substrate on a moving table, a dispensing area, and a detection area. The installation area, the dispensing area, and the detection area are arranged on the same straight line, An analytical apparatus comprising: a guide means arranged along the same straight line; and a moving table guided by the guide means.

2. In the analytical device according to claim 1, It also has a drainage area, The analytical device, wherein the installation area, the dispensing area, the detection area and the drainage area are arranged on the same straight line.

3. In the analytical device according to claim 2, It also has a mixing area. The analytical device, wherein the installation area, the dispensing area, the detection area, the drainage area and the mixing area are arranged on the same straight line.

4. In the analytical device according to claim 1, The dispensing area is provided with a dispensing nozzle that is movable in a direction substantially perpendicular to the straight line.

5. In the analytical device according to claim 1, The analytical device is characterized in that the tip housed in the reagent cartridge is returned to its original housing position after dispensing the specimen or the reagent to the biochemical reaction substrate by a dispensing nozzle.

6. In the analytical device according to claim 1, the reagent cartridge contains the sample and a reaction reagent and a washing solution as the reagent, and is equipped with at least three chips; The at least three tips are chips for aspirating and injecting the sample, the reaction reagent, and the washing solution using a dispensing nozzle.

7. In the analytical device according to claim 6, The reagent cartridge is an analytical device comprising, as the reaction reagent, a reagent containing a labeled antibody and a reagent containing a luminescent material.

8. In the analytical device according to claim 6, The washing liquid is a liquid for washing the specimen or the reaction reagent dispensed onto the biochemical reaction substrate.

9. In the analytical device according to claim 1, An analytical device characterized in that a barcode reading area is also arranged on the same straight line.

10. In the analytical device according to claim 2, The analytical device is characterized in that the installation area is arranged at either end of the same straight line.

11. In the analytical device according to claim 10, The analytical device is characterized in that the drainage area is located at either other end of the same straight line.

12. In the analytical device according to claim 2, The analytical device is characterized in that the drainage area is provided with a stopper formed in the same straight line, and further includes a tilting mechanism that tilts the biochemical reaction substrate by contacting the stopper.

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