Liquid composition for allergen reduction

Abstract

To provide a liquid composition for allergen reduction comprising copper ions, with enhanced effectiveness in reducing allergens.SOLUTION: A liquid composition for allergen reduction comprises copper ions, where the concentration of the copper ions is 0.1 ppm or more and 50 ppm or less, and the pH is 9.0 or more and 11.0 or less.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a liquid composition for reducing allergens. [Background technology]

[0002] Allergens such as mites and pollen cause allergic diseases such as allergic asthma, allergic rhinitis, conjunctivitis, atopic dermatitis, etc. Therefore, there is a demand for technology to reduce allergens in the living environment in order to prevent or reduce allergic diseases.

[0003] Patent Document 1 discloses a composition containing a metal carboxylate as an allergen reducing agent, and the metals disclosed in the metal carboxylate are rare earth metals, zinc, copper, and zirconium (Patent Document 1). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] JP 2015-178582 A Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a liquid composition for reducing allergens, which contains copper ions and has an improved allergen reducing effect. [Means for solving the problem]

[0006] The present invention relates to the following liquid composition. [1] A liquid composition containing copper ions, The concentration of the copper ions is 0.1 ppm or more and 50 ppm or less, A liquid composition for reducing allergens, having a pH of 9.0 or more and 11.0 or less. [2] The liquid composition described in [1], wherein the allergen is at least one of a mite allergen and a pollen allergen. [3] The liquid composition according to [1] or [2], which is a liquid composition contained in a spray container. Effect of the Invention

[0007] According to the present invention, a liquid composition having an improved allergen reducing effect can be provided. [Brief description of the drawings]

[0008] [Figure 1] FIG. 13 is a graph showing the results of Test Example 3. [Diagram 2] FIG. 13 is a diagram showing the results of Test Example 4. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] [Liquid composition] The liquid composition for reducing allergens of the present invention (hereinafter also referred to as "the liquid composition") contains copper ions, the concentration of the copper ions is 0.1 ppm or more and 50 ppm or less, and the pH is 9.0 or more and 11.0 or less. The liquid composition may contain any component other than the copper ions. The liquid composition contains a solvent. The liquid composition exhibits an excellent allergen reduction effect.

[0010] The pH of the present liquid composition is 9.0 or more and 11.0 or less, and preferably 9.5 or more and 11.0 or less, thereby achieving an excellent allergen reducing effect.

[0011] <Copper ions> The liquid composition contains copper ions (Cu 2+ and / or Cu +). In this liquid composition, the concentration of copper ions is 0.1 ppm or more and 50.0 ppm or less, preferably 0.5 ppm or more and 10.0 ppm or less. By having a copper ion concentration of 0.1 ppm or more, an excellent allergen reduction effect can be obtained. The source of copper ions is not limited, and can be added, for example, as copper gluconate, copper sulfate, copper malate, copper chloride, or copper lactate. The source of copper ions may be commercially available copper ion water or copper ion water generated by a copper ion generator. An example of commercially available copper ion water is Algicut (manufactured by Nippon Ion Co., Ltd.), and an example of a commercially available copper ion generator is Copper Shimasho (manufactured by Nippon Ion Co., Ltd.).

[0012] <Solvent> In the present liquid composition, it is preferable to use water as a solvent. It is preferable that the water is close to pure water, but it is sufficient to use water of a grade that does not affect the blended components or that does not substantially contain compounds that may be allergens as impurities. Specifically, sterilized water sterilized with chlorine or ion-exchanged water from which trace amounts of metal components such as calcium have been removed can be used.

[0013] The liquid composition may contain a solvent other than water. Examples of the solvent that the liquid composition may contain include water-soluble organic solvents. By including a water-soluble organic solvent, the drying of the composition can be accelerated after the liquid composition is brought into contact with the target surface, and the stability of the liquid composition can be improved. Examples of water-soluble organic solvents used for such purposes include ethanol, cyclohexane, 2-butanol, cyclohexanol, cyclohexanenone, toluene, 1-butanol, 2-butanol, 1-propanol, 2-propanol, 1-hexanol, hexane, 1-heptanol, 1-pentanol, and 2-pentanol, and alcohol compounds having 2 to 7 carbon atoms are preferred. In particular, ethanol, 1-propanol, and 2-propanol are most preferred in terms of the allergen removal effect.

[0014] <Optional ingredients> The present liquid composition may optionally contain known disinfectants, pH adjusters, chelating agents, preservatives, thickeners, fragrances, deodorants, scale inhibitors, antifoaming agents, antistatic agents, fabric softeners, and the like.

[0015] The disinfectant is not limited as long as it is dissolved in the liquid composition, and examples of the disinfectant include benzalkonium chloride, benzethonium chloride, chlorhexidine gluconate, grapefruit seed extract, triclosan, ε-polylysine, isopropyl methylphenol, catechin, and flavonoids.

[0016] The pH adjuster is not limited as long as it can adjust the pH of the liquid composition to 9.0 or more and 11.0 or less, and examples thereof include ammonium phosphate, ammonium hydrogen phosphate, sodium phosphate, sodium hydrogen phosphate, potassium phosphate, potassium hydrogen phosphate, amino acids, urea, organic acids or salts thereof, etc. Two or more of these pH adjusters may be mixed and used, and are preferably used within the range of 0.001 to 5% by mass, more preferably within the range of 0.01 to 2% by mass.

[0017] <Allergens to be reduced> An allergen means a substance that causes allergies, and is often composed of proteins or glycoproteins. When an allergen binds to an IgE antibody and activates mast cells, inflammation occurs. There are no particular limitations on the allergens that can be reduced, and examples include mite allergens derived from mites, pollen allergens, mold allergens, and dog and cat allergens. In particular, mite allergens and pollen allergens are important allergens that need to be reduced. It is said that mite allergens account for approximately 70% of indoor allergens. Among them, Dermatophagoides pteronyssinus, a member of the dust mite family that lives in indoor dust, accounts for a large proportion as a source of allergens.

[0018] Among the house dust mites, Dermatophagoides farinae and Dermatophagoides pteronyssinus are representative species, and Der f1 / Der p1 derived from their feces are the major allergens in allergic diseases. In addition to these, Der f2 / Der p2 derived from their carcasses are also allergens.

[0019] Examples of allergens derived from plant pollen include the pollen allergen (Cry j1) derived from Japanese cedar (Cryptomeria japonica), as well as pollen allergens from Japanese cypress, ragweed, mugwort, orchard grass, Japanese silvergrass, and the like.

[0020] Reduction of an allergen means that the antibody no longer reacts with the allergen, and includes, for example, removal, capture, adsorption, decomposition, denaturation, or coating of the reactive site of the allergen.

[0021] [Usage form] The present liquid composition can be provided as an allergen reducing agent. Examples of the usage form of the present liquid composition include a container-packed liquid composition obtained by filling a container with the present liquid composition, a spray container-packed liquid composition obtained by filling a container equipped with a spray device with the present liquid composition, and an allergen reducing sheet obtained by impregnating a flexible sheet with the present liquid composition.

[0022] The containerized liquid composition can be used in a cleaning method in which the liquid composition in the container is impregnated into a sheet or fabric and used to wipe a target surface. The allergen-reduced sheet can be used in a cleaning method in which the target surface is wiped with the sheet. The liquid composition can be provided as a liquid composition labeled with the fact that it is used for carrying out such a specific cleaning method, and as an allergen-reduced sheet labeled with the same.

[0023] The liquid composition in a spray container is used in a method for reducing allergens by spraying into the air where allergens are suspended, as well as in a method for directly spraying onto a treatment target to reduce allergens on the target surface, etc. This liquid composition can be provided as a liquid composition in a spray container labeled to the effect that it is for use in an allergen reduction method in which the composition is sprayed into space.

[0024] Examples of spray devices include, but are not limited to, aerosol types that use propellants such as carbon dioxide, LPG, DME, and nitrogen gas, and trigger-type spray devices that spray by mixing with manually compressed air, with trigger-type spray devices being preferred.

[0025] This liquid composition can be used by spraying or applying it to interior items such as indoor floors, carpets, sofas, wallpaper, curtains, etc., bedding such as futon coverings, futon covers, futon fillings, sheets, pillowcases, mats, etc., automobile parts such as car seats and car mats, stuffed toys, etc. In addition, by processing nonwoven fabrics, wallpapers, filter materials, fibers or textile products, architectural interior materials, etc. so that they contain this liquid composition, it is possible to provide filter materials, nonwoven fabrics such as masks, fibers or textile products, floor materials, ceiling materials, architectural interior materials, etc., which have the function of reducing allergens. EXAMPLES

[0026] The present invention will be described in more detail with reference to test examples, but the present invention is not limited to these. Note that all percentages shown below are by mass.

[0027] [Test Example 1] (1) Preparation of protein solution Deionized water was added to the breeding medium of Dermatophagoides farinae so that the protein concentration became 100 mg / mL, and the mixture was mixed at room temperature for 1 hour to prepare a protein solution.

[0028] (2) Preparation of sample solution (2-1) Advance preparation The following (a) and (b) were prepared for use in preparing the sample solution. (a) Buffer: Na2HPO4-NaOH (pH8.0, 9.0, 10.0, 11.0) (b) Copper ion water (produced using Algicut, manufactured by Nippon Ion Co., Ltd.)

[0029] (2-2) Preparation of sample solution The sample solutions were prepared by adding copper ion water (b) to buffer solutions (a) of each pH level, and adding the protein solution prepared above so that the final protein concentration was 300 μg / mL, and reacting at room temperature for 30 minutes. The copper ion water (b) was added so that the copper ion concentration in the sample solutions was the concentration shown in Table 1. The Der f1 concentration in each sample solution was also measured.

[0030] (2-3) Preparation of reference solution X1 The standard solution was prepared by adding the protein solution prepared above to each pH buffer (a) so that the final protein concentration was 300 μg / mL, and reacting at room temperature for 30 minutes. This was designated as standard solution X1.

[0031] (2-4) Preparation for ELISA measurement After the reaction, 111 μL of 0.5 M EDTA was added to the sample solution and standard solution X1, and the solution was left to stand for 15 minutes, and then appropriately diluted with TBS-T (25 mM Tris, 150 mM NaCl, 0.02% Tween 20, pH 7.5) to prepare it for ELISA measurement.

[0032] (3) ELISA measurement Anti-Mite (Dermatophagoides farinae, Allergen, Mouse-Mono (6A8)) was used as a capture antibody. <anti-derf1>) (INDOOR, 1:2000) was added to each well of a 96-well plate (Nunc) at 100 μL each, and immobilized overnight at 4°C. After washing with TBS-T, blocking was performed at room temperature for 1 hour using TBS-T containing 1% BSA. After washing the wells with TBS-T, 100 μL of each sample solution or each standard solution X1 prepared for ELISA measurement was added to the wells and incubated at 37°C for 1 hour. After washing the wells with TBS-T, the detection antibodies were Anti-Mite (Dermatophagoides pteronyssinus / D.farinae), Allergen, Mouse-Mono (4C1) (INDOOR, 1:2000) biotin-labeled with Biotin Labeling Kit - NH2 (Dojindo Laboratories), and 100 μL each was added to each well and incubated at room temperature for 2 hours. After washing the wells with TBS-T, 100 μL of HRP-labeled streptavidin (Abcam, 1:10000) was added to each well and incubated at room temperature for 30 minutes. After washing the wells with TBS-T, a color reaction was performed. The color reaction was performed using SureBlue TMB substrate (KPL), and the reaction was stopped with TMB stop solution (KPL). The absorbance at a wavelength of 450 nm was measured using an EMax Plus Microplate Reader (Molecular Device) and SoftMax Pro 6.5.1 (Molecular Device).

[0033] (4) Quantification of Der f1 concentration and protein concentration The Der f1 concentration in each sample solution and each standard solution was quantified based on the calibration curve obtained from the absorbance of the standard solution for the calibration curve. The total protein concentration in each sample solution and each standard solution was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific).

[0034] (5) Calculation of the reduction amount of Der f1 The reduction in Der f1 was calculated by subtracting the Der f1 concentration of each sample solution from the Der f1 concentration of each standard solution X. Table 1 shows the calculation results.

[0035] [Table 1]

[0036] [Test Example 2] (1) Preparation of protein solution Mite-Df Feces AG (manufactured by Cosmo Bio Co., Ltd.) was treated according to the attached instructions to prepare a mite allergen solution (Der f1 solution).

[0037] (2) Preparation of sample solution (2-1) Advance preparation The following (a) and (b) were prepared for use in preparing the sample solution. (a) Buffer: Na2HPO4-NaOH (pH8.0, 9.0, 10.0, 11.0) (b) Copper ion water (produced using Algicut, manufactured by Nippon Ion Co., Ltd.)

[0038] (2-2) Preparation of sample solution The sample solution was prepared by adding copper ion water (b) to each pH buffer solution (a) and adding the Der f1 solution prepared above so that the final concentration of Der f1 was 100 ng / mL, and reacting at room temperature for 30 minutes. The copper ion water (b) was added so that the copper ion concentration in the sample solution was the concentration shown in Table 2.

[0039] (2-3) Preparation of reference solution X2 The standard solution was prepared by adding the Der f1 solution prepared above to the buffer solution (a) of each pH so that the final concentration of Der f1 was 100 ng / mL, and reacting at room temperature for 30 minutes. This was designated as standard solution X2.

[0040] Using the same method as in Test Example 1, ELISA measurements were performed on each sample solution and standard solution X2, and the Der f1 concentration was quantified based on the results. The value obtained by subtracting the Der f1 concentration of each sample solution from the Der f1 concentration of each standard solution X2 was then calculated as the amount of reduction in Der f1. The calculation results are shown in Table 2. In Table 2, "NT" indicates that no measurement was performed.

[0041] [Table 2]

[0042] [Test Example 3] (1) Preparation of sample solution (1-1) Advance preparation The following items (a) to (c) were prepared for use in preparing the sample solution. (a) Buffer: Na2HPO4-NaOH (pH8.0, 9.0, 9.5, 10.0, 11.0) (b) Copper ion water (produced using Algicut, manufactured by Nippon Ion Co., Ltd.) (c) Trypsin solution

[0043] (1-2) Preparation of sample solution The sample solution was prepared by adding copper ion water (b) to each pH buffer solution (a) and then adding trypsin solution (c) so that the final trypsin concentration was 300 μg / mL, and reacting the solution at room temperature for 15 minutes while stirring with a rotator. The copper ion water (b) was added so that the copper ion concentration in the sample solution was as shown in Table 2.

[0044] (1-3) Preparation of reference solution Y A reference solution was prepared by adding trypsin solution (c) to each pH buffer solution (a) so that the final trypsin concentration was 300 μg / mL, and the mixture was allowed to react at room temperature for 15 minutes while being stirred on a rotator.

[0045] (2) Measurement of trypsin concentration 100 μL of each sample solution or each standard solution Y was mixed with 25 mM phosphate buffer pH 7.0 containing 10 mM EDTA. 50 μL of the mixed solution was added to 150 μL of a solution containing 1000 μL of 100 mM citrate buffer containing 20 mM L-cysteine ​​and 10 mM EDTA and 800 μL of 1.85 mM Bz-Arg-pNA, and the absorbance was immediately measured using EMax Plus Microplate Reader (Molecular Device) and SoftMax Pro 6.5.1 (Molecular Device) (Kinetics mode, wavelength 405 nm, total measurement time 10 minutes, each measurement interval 1 minute, Vmax).

[0046] The absorbance of solutions containing only trypsin at 0, 25, 50, 100, 200, and 300 μg / mL was measured in the same manner as above to create a calibration curve, and the residual trypsin concentration in each sample solution or each standard solution Y was calculated. The amount of trypsin reduced in each sample solution was calculated using the following formula with standard solution Y at each pH as the standard. The calculation results are shown in Table 3. Figure 1 is a diagram in which the calculated values ​​in Table 3 are plotted. Trypsin reduction concentration of each sample solution (μg / mL) = remaining trypsin concentration of standard solution Y - remaining trypsin concentration of each sample solution

[0047] [Table 3]

[0048] [Test Example 4] Test Example 4 was performed in the same manner as Test Example 3, except that the reaction time during preparation of the sample solution and standard solution Y was changed from 15 minutes to 30 minutes. The amount of trypsin reduced in each sample solution is shown in Table 4. Figure 2 is a diagram in which the calculated values ​​in Table 4 are plotted.

[0049] [Table 4]

Claims

1. A liquid composition containing copper ions, The concentration of the copper ions is 0.1 ppm or more and 50 ppm or less, A liquid composition for reducing allergens, having a pH of 9.0 or more and 11.0 or less.

2. The liquid composition according to claim 1 , wherein the allergen is at least one of a mite allergen and a pollen allergen.

3. The liquid composition according to claim 1 or 2, which is a liquid composition contained in a spray container.

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