Composition for food and beverage use

Abstract

To provide a composition for food and beverage use which is effective in muscle mass maintenance and / or increase, and in muscle strength maintenance and / or enhancement.SOLUTION: Provided is a composition for food and beverage use, which contains a protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidins. Also provided is a composition for food and beverage use, which contains a protein and kaempferol as a polyphenol compound, and further contains at least one additional polyphenol compound selected from the group consisting of quercetin, caffeic acid, and ferulic acid.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a food or beverage composition containing a protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin. [Background technology]

[0002] Traditionally, nutritional supplements made by powdering protein and taking it with water or other liquids were only consumed by a select few athletes and were not very familiar to the general public. However, in recent years, due to growing interest in health and other factors, opportunities for the general public to consume such supplements have been increasing.

[0003] In recent years, dietary supplements mainly composed of protein are often taken for the purpose of weight gain, and a dietary supplement containing low molecular weight protein, fructose, and black pepper extract is known as a dietary supplement that can effectively increase weight (Patent Document 1). However, there has been insufficient research on combinations of proteins and specific ingredients that are effective in maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] JP 2019-149984 A DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a food and drink composition that is excellent in maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. [Means for solving the problem]

[0006] In order to solve the above problems, the applicant has conducted extensive research into various substances and has surprisingly found that a food composition having excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength can be obtained by containing at least one polyphenol compound selected from the group consisting of kaempferol, gallic acid, and proanthocyanidin as a protein and a polyphenol compound. It has also been found that a food composition having particularly excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength can be obtained by further containing at least one polyphenol compound selected from the group consisting of quercetin, caffeic acid, and ferulic acid in a food composition containing protein and kaempferol. The present invention has been completed based on these findings.

[0007] The outline of the present invention is as follows. Food and drink composition containing protein and at least one polyphenol compound selected from kaempferol, gallic acid and proanthocyanidin An edible composition containing protein and kaempferol, further containing at least one polyphenol compound selected from the group consisting of quercetin, caffeic acid and ferulic acid. The protein content in the composition is 20% by mass or more. <1> or <2> The food or beverage composition described above. For maintaining and / or increasing muscle mass; <1> or <2> The food or beverage composition described above. For maintaining and / or improving muscle strength; <1> or <2> The food or beverage composition described above. Effect of the Invention

[0008] The composition of the present invention is excellent in the effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. In addition, since the composition of the present invention has a mitochondrial activation effect, excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength can be expected. BEST MODE FOR CARRYING OUT THEINVENTION

[0009] The composition for food or drink of the present invention will be described below. Note that the present invention is not limited to the following embodiments.

[0010] [1. Protein] The present invention uses protein as an essential component. The protein used in the present invention can be an animal protein, a vegetable protein, a microbial protein, or a combination thereof. Examples of animal proteins include milk-derived proteins such as whey protein (milk serum) and casein protein, egg-derived proteins such as chicken eggs, egg yolks, and egg whites, silk proteins, and insect proteins. Examples of vegetable proteins include cereal-derived proteins such as wheat-derived proteins, soybean-derived proteins, rice-derived proteins, pigeon oat-derived proteins, oat-derived proteins, corn-derived proteins, pea-derived proteins, broad bean-derived proteins, and chickpea proteins, almond-derived proteins, potato-derived proteins, potato-derived proteins such as potato waste, hemp seed-derived proteins, sesame-derived proteins, pumpkin seed-derived proteins, cranberry-derived proteins, peach-derived proteins, and algae-derived proteins. Examples of microbial-derived proteins include fungal-derived proteins such as yeast-derived proteins, koji-derived proteins, and mushroom-derived proteins, and bacterial-derived proteins such as natto bacteria-derived proteins and lactic acid bacteria-derived proteins. In the present invention, milk-derived proteins such as whey protein and casein protein, egg-derived proteins such as chicken eggs, egg yolks, and egg whites, wheat-derived proteins, soybean-derived proteins, rice-derived proteins, pea-derived proteins, fungal-derived proteins, and bacterial-derived proteins are preferred, and milk-derived proteins such as whey protein and casein protein, egg-derived proteins such as chicken eggs, egg yolks, and egg whites, soybean-derived proteins, pea-derived proteins, fungal-derived proteins, and bacterial-derived proteins are more preferred, and from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, whey protein, casein protein, egg-derived proteins such as chicken eggs, egg yolks, and egg whites, soybean-derived proteins, pea-derived proteins, fungal-derived proteins, and bacterial-derived proteins are particularly preferred. The proteins used in the present invention are hydrolyzed (peptides), concentrated (concentrates), and high-purity (isolates). In addition, in the present invention, protein-containing raw materials containing components other than proteins such as excipients can also be used.The average molecular weight (weight average molecular weight) of the protein is not particularly limited, but is, for example, preferably 300 to 100,000 Da, and more preferably 500 to 80,000 Da.

[0011] The protein content in the composition of the present invention is not particularly limited, but from the viewpoint of exerting an excellent effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, the protein-containing raw material preferably contains 20 to 95% by mass, more preferably 30 to 93% by mass, particularly preferably 40 to 91% by mass, and particularly preferably 50 to 90% by mass, in terms of dry weight. The protein content in the raw material used is preferably 40% by mass or more, more preferably 50% by mass or more, in terms of exerting an effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, and particularly preferably 60% by mass or more.

[0012] [2. Polyphenol compounds] <Kaempferol> The present invention uses kaempferol as an essential ingredient. Kaempferol is a compound classified as a flavonol, which belongs to the flavonoids of polyphenolic compounds. The kaempferol used in the food and beverage composition of the present invention may be an aglycone or a glycoside thereof, and is not limited by its form or production method. Examples of kaempferol glycosides include the following: (1) kaempferol-3-O-[β-D-glucopyranosyl-(1-3)-α-L-rhamnopyranosyl-(1-6)-β-D-glucopyranoside], (2) kaempferol-3-O-[β-D-glucopyranosyl-(1-3)-α-L-rhamnopyranosyl-(1-6)-β-D-galactopyranoside]. For example, kaempferol extracted by a known method from a plant known to be rich in kaempferol can be used as it is, or a synthetic product can be used. The plant-derived kaempferol may be used as it is, or may be converted to its aglycone by known methods (e.g., enzymatic treatment). In order to incorporate an effective amount, it is preferable to use one whose content has been increased by operations such as concentration and purification. In this case, known concentration and purification methods can be used. In the present invention, only one type of kaempferol may be used, or two or more types may be used. In the present invention, commercially available kaempferol can be used.

[0013] Natural products containing kaempferol that can be used in the present invention include, but are not limited to, plants of the Zingiberaceae, Rhamnaceae, Geraniaceae, Araliaceae, Fabaceae, and Theaceae families, with Zingiberaceae, Araliaceae, and Fabaceae plants being preferred, and Araliaceae and Fabaceae being more preferred.

[0014] The processed natural product containing kaempferol is not particularly limited, but may be, for example, a crushed product, a squeezed juice, an extract, etc. Crushed products may be, for example, powder, granules, etc. Extracts are obtained by extracting natural products with a solvent. The solvent may be, for example, water, an organic solvent, or a water-containing organic solvent (water-containing alcohol such as water-containing ethanol). The extraction method may be, for example, a solid-liquid extraction method such as a warm extraction method or a supercritical fluid extraction method, as long as it is a method that is generally acceptable for extracting natural product components. The squeezed juice or extract may be in a liquid form, but may also be used as a paste or a dry powder. When making it into a paste or a dry powder, it may be produced by using only the product itself, or may be processed together with an excipient. The processed product obtained may be further subjected to chemical treatment, enzyme treatment, or purification treatment.

[0015] The blending ratio of kaempferol in the composition of the present invention is not particularly limited, and the content can be appropriately set within a wide range depending on various conditions such as the purpose, shape, and subject of use. For example, when the composition of the present invention is a solid preparation (powder, granule, tablet, capsule, chewable, etc.), the content is preferably 0.000001 to 50% by mass, more preferably 0.00001 to 30% by mass, and particularly preferably 0.001 to 10% by mass from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. Also, for example, when the composition of the present invention is a liquid preparation (liquid, gel, jelly, paste, etc.), the content is preferably 0.0000001 to 10% by mass, more preferably 0.000001 to 5% by mass, and particularly preferably 0.00001 to 1% by mass from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength.

[0016] The content of kaempferol in the dietary composition of the present invention can be measured by a method suitable for the condition of the sample among the commonly known methods for analyzing kaempferol. For example, it can be analyzed by liquid chromatography. As an example, it can be measured by a high-performance liquid chromatograph equipped with a mass spectrometer, and the analytical column is ODS-AM (4.6 x 250 mm) manufactured by YMC Co., Ltd., and the mobile phase is a solvent of a mixture of water and acetic acid (1000:5) and acetonitrile in a ratio of 70:30, and the column temperature is 40°C and the flow rate is 0.7 mL / min. In addition, when measuring, appropriate treatment may be performed as necessary, such as removing impurities in the sample to match the separation ability of the device.

[0017] <Gallic acid> The present invention uses gallic acid as an essential component. Gallic acid refers to 3,4,5-trihydroxybenzene carboxylic acid. The salt of gallic acid used in the present invention is not particularly limited, but may be, for example, an alkali metal salt. As the gallic acid or its salt used in the present invention, gallic acid is preferable from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. As the gallic acid used in the present invention, it is not particularly limited, but may be a synthetic product synthesized by a method generally known to those skilled in the art, a natural product containing gallic acid, or a processed product of the natural product. In the present invention, a synthetic product synthesized by a method generally known to those skilled in the art, a natural product containing gallic acid, or a processed product of the natural product is preferable, a natural product containing gallic acid or a processed product of the natural product is more preferable, and a processed product of the natural product containing gallic acid is particularly preferable from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. In the present invention, only one type of gallic acid may be used, or two or more types of gallic acid may be used. In the present invention, commercially available gallic acid can be used.

[0018] The natural product containing gallic acid used in the present invention is not particularly limited, and examples thereof include plants of the Anacardiaceae family, Fagaceae family, Fabaceae family, Theaceae family, and Combriaceae family, with Fabaceae, Theaceae family, and Combriaceae family plants being preferred, and Theaceae and Combriaceae families being more preferred.

[0019] The processed natural product containing gallic acid is not particularly limited, but may be, for example, a crushed product, a squeezed juice, an extract, etc. The crushed product may be, for example, a powder, a granule, etc. The extract is obtained by extracting the natural product with a solvent. The solvent is not particularly limited, but may be, for example, water, an organic solvent, or a water-containing organic solvent (water-containing alcohol such as water-containing ethanol). The extraction method is not particularly limited as long as it is a method that is usually acceptable for extracting natural product components, but may be, for example, a solid-liquid extraction method such as a heating extraction method or a supercritical fluid extraction method. The squeezed juice or extract may be in a liquid form, but may also be used as a paste or a dry powder. When it is made into a paste or a dry powder, it may be produced by using only the product itself, or may be processed together with an excipient. The obtained processed product may be further subjected to a chemical treatment, an enzyme treatment, or a purification treatment.

[0020] The blending ratio of gallic acid in the composition for food or drink of the present invention is not particularly limited, and the content can be appropriately set within a wide range depending on various conditions such as purpose, shape, and subject of use. For example, when the composition of the present invention is a solid preparation (powder, granule, tablet, capsule, chewable, etc.), the content is preferably 0.000001 to 50% by mass, more preferably 0.00001 to 30% by mass, and particularly preferably 0.001 to 10% by mass from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. Also, for example, when the composition of the present invention is a liquid preparation (liquid, gel, jelly, paste, etc.), the content is preferably 0.0000001 to 10% by mass, more preferably 0.000001 to 5% by mass, and particularly preferably 0.00001 to 1% by mass from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength.

[0021] The content of gallic acid in the composition for food or drink of the present invention can be measured by an analytical method suitable for the condition of the measurement sample among commonly known analytical methods for gallic acid. For example, it can be analyzed by liquid chromatography. As an example, it can be measured by a high-performance liquid chromatograph equipped with an ultraviolet absorption detector, and the analytical column is Unison UK-C18HT (3×100 mm) manufactured by Imtakt, and the mobile phase is a 1% by volume aqueous solution of formic acid (mobile phase A) and a 1% by volume solution of formic acid in acetonitrile (mobile phase B). The column temperature can be 45° C. and the flow rate can be 0.7 mL / min. The gradient conditions can be as follows. In addition, when measuring, appropriate treatments such as removing impurities in the sample to match the separation ability of the device may be performed as necessary.

[0022] [Table A]

[0023] <Proanthocyanidin> The present invention uses proanthocyanidin as an essential component. Proanthocyanidin refers to a group of compounds consisting of condensation polymers with a degree of polymerization of 2 or more, whose constituent units are flavan-3-ol and / or flavan-3,4-diol. Proanthocyanidin is a strong antioxidant produced by plants, and is contained intensively in the leaves, bark, fruit skin and seeds of plants. This proanthocyanidin is a substance that cannot be produced in the human body. As proanthocyanidin, particularly proanthocyanidin containing a large amount of condensation polymers with a low degree of polymerization is preferable. As the condensation polymers with a low degree of polymerization, condensation polymers (di-30-mers) with a degree of polymerization of 2-30 are preferable, condensation polymers (di-10-mers) with a degree of polymerization of 2-10 are more preferable, and condensation polymers (di-4-mers) with a degree of polymerization of 2-4 are even more preferable. Proanthocyanidins of condensation polymers (di-4-mers) with a degree of polymerization of 2-4 are particularly easily absorbed into the body. In this specification, polymers with a degree of polymerization of 2 to 4 are referred to as oligomeric proanthocyanidins (hereinafter referred to as "OPCs").

[0024] The proanthocyanidins used in the composition for food and drink of the present invention can be ground bark, fruit, seeds, seed coats, etc. of the above plants. Among the plants containing proanthocyanidins, pine bark or black soybean seed coat is rich in OPCs, and is therefore preferably used as a raw material for proanthocyanidin-containing plants. As the pine bark, the bark of plants belonging to the order Pinales, such as French maritime pine (Pinus Martima), larch, black pine, red pine, Japanese white pine, Korean pine, Japanese stone pine, Ryukyu pine, Japanese white pine, white pine, and Aneda in the Quebec region of Canada, is preferably used.

[0025] The method for extracting the proanthocyanidins is not particularly limited, but the method of extracting the plant body with water or an organic solvent is preferred. When using water, it is preferred to use warm water or hot water. In order to improve extraction efficiency, it is preferred to add salts such as sodium chloride to these waters. As the organic solvent used for extraction, organic solvents that are acceptable for the production of food or medicines are used, such as ethanol and edible oils and fats. These waters and organic solvents may be used alone or in combination. Among these, water, hot water, ethanol, and aqueous ethanol are particularly preferred.

[0026] When hot water (meaning water having a temperature of 50° C. or higher) is used as the solvent, the temperature of the hot water is preferably 50° C. to 120° C., and more preferably 70° C. to 100° C., from the viewpoint of the extraction efficiency of proanthocyanidins.

[0027] As a specific method for extracting the proanthocyanidins, for example, 1 part by mass of hot water at 50°C to 120°C, preferably 70°C to 100°C, is added to 1 part by mass of the dry mass of the pine bark, and then the pine bark extract having high physiological activity and excellent water solubility can be obtained. In addition, a heating extraction method, a supercritical fluid extraction method, etc. may be used. In addition, supercritical fluid extraction by an entrainer addition method may be performed. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, or other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, or ketones are added to the supercritical fluid at about 2 mass / volume% to 20 mass / volume%, and the obtained extraction fluid is used for supercritical fluid extraction, thereby dramatically increasing the solubility of the target extract such as OPCs and catechins in the extraction solvent, or enhancing the selectivity of separation, and thus efficiently obtaining proanthocyanidins. The extraction of proanthocyanidins from the plant body may be performed by combining a plurality of extraction methods. By combining multiple extraction methods, it is possible to obtain proanthocyanidins with various compositions.

[0028] The proanthocyanidin obtained by the extraction method may be purified for the purpose of increasing the proanthocyanidin content. A solvent is usually used for purification, but it can also be purified by a method that does not use a solvent, such as ultrafiltration, or a column method or batch method using an adsorbent carrier such as Diaion HP-20, Sephadex-LH20, or chitin. The proanthocyanidin is an extract obtained by extracting the plant body with a solvent, or the extract is further concentrated or diluted (proanthocyanidin-containing plant extract). The form is not particularly limited, and may be, for example, a solution, a suspension, a semisolid (e.g., gel, paste, etc.), or a solid (e.g., powder, granules, etc.). When the proanthocyanidin-containing plant extract is a powder, it can be obtained by drying the proanthocyanidin-containing plant extract by a known method such as freeze-drying. The proanthocyanidin-containing plant extract may be appropriately extracted from the plant body by the above-mentioned extraction method, or a commercially available product may be used. Commercially available products of the proanthocyanidin-containing plant extract include, for example, Flavangenol (registered trademark, manufactured by Toyo Shinyaku Co., Ltd.).

[0029] The blending ratio of proanthocyanidins in the composition for food and drink of the present invention is not particularly limited, and the content can be appropriately set in a wide range depending on various conditions such as purpose, shape, and target of use. For example, when the composition of the present invention is a solid agent (powder, granule, tablet, capsule, chewable, etc.), the content is preferably 0.000001 to 50% by mass, more preferably 0.00001 to 30% by mass, and from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, 0.001 to 10% by mass is particularly preferred. Also, for example, when the composition of the present invention is a liquid agent (liquid, gel, jelly, paste, etc.), the content is preferably 0.0000001 to 10% by mass, more preferably 0.000001 to 5% by mass, and from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, 0.00001 to 1% by mass is particularly preferred.

[0030] [3. Other polyphenol compounds] <Quercetin, caffeic acid, ferulic acid> In the present invention, it is preferable to further include at least one selected from quercetin, caffeic acid and ferulic acid in addition to protein and kaempferol in order to enhance the effects of the present invention. Quercetin, caffeic acid and ferulic acid are compounds classified as polyphenol compounds. The quercetin, caffeic acid and ferulic acid used in the food and beverage composition of the present invention may be aglycones or glycosides thereof, and are not limited by their form or production method. For example, quercetin, caffeic acid or ferulic acid extracted by a known method from a plant known to contain a large amount of quercetin, caffeic acid or ferulic acid may be used as it is, or a synthetic product may be used. The quercetin, caffeic acid and ferulic acid derived from the plant may be used as it is, or may be converted into its aglycone by a known method (for example, by enzyme treatment). In order to blend an effective amount, it is preferable to use one in which the content has been increased by operations such as concentration and purification. In this case, known concentration and purification methods can be used. In the present invention, only one type of quercetin, caffeic acid and ferulic acid may be used, or two or more types may be used. In the present invention, commercially available quercetin, caffeic acid and ferulic acid can be used.

[0031] Natural products containing quercetin, caffeic acid and ferulic acid used in the present invention are not particularly limited, and include plants of the Zingiberaceae, Rhamnaceae, Geraniaceae, Araliaceae, Fabaceae and Theaceae families, with Zingiberaceae, Araliaceae and Fabaceae plants being preferred, and Araliaceae and Fabaceae plants being more preferred.

[0032] The processed natural product containing quercetin, caffeic acid and ferulic acid is not particularly limited, but may be, for example, a crushed product, a squeezed juice, an extract, etc. Crushed products include powders, granules, etc. The extract is obtained by extracting the natural product with a solvent. The solvent is not particularly limited, but may be, for example, water, an organic solvent, or a water-containing organic solvent (water-containing alcohol such as water-containing ethanol). The extraction method is not particularly limited as long as it is a method that is usually acceptable for extracting natural product components, but may be, for example, a solid-liquid extraction method such as a warm extraction method or a supercritical fluid extraction method. The squeezed juice or extract may be in a liquid form, but may also be used as a paste or a dry powder. When making it into a paste or a dry powder, it may be produced by using only the product itself, or may be processed together with an excipient. The obtained processed product may be further subjected to a chemical treatment, an enzyme treatment, or a purification treatment.

[0033] The blending ratio of quercetin, caffeic acid and ferulic acid in the composition of the present invention is not particularly limited, and the contents can be appropriately set within a wide range depending on various conditions such as the purpose, shape, and subject of use. For example, when the composition of the present invention is a solid preparation (powder, granules, tablets, capsules, chewables, etc.), the total content of quercetin, caffeic acid and ferulic acid is preferably 0.0000001 to 50% by mass, more preferably 0.000001 to 30% by mass, and particularly preferably 0.0001 to 10% by mass from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength. Furthermore, for example, when the composition of the present invention is a liquid preparation (liquid, gel, jelly, paste, etc.), the total content of quercetin, caffeic acid and ferulic acid is preferably 0.00000001 to 10 mass%, more preferably 0.0000001 to 5 mass%, and from the viewpoint of the effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, it is particularly preferably 0.000001 to 1 mass%.

[0034] The contents of quercetin, caffeic acid and ferulic acid in the food and beverage composition of the present invention can be measured by a method suitable for the condition of the measurement sample among the commonly known methods for analyzing quercetin, caffeic acid and ferulic acid. For example, it can be analyzed by liquid chromatography. As an example, quercetin can be measured by a high-performance liquid chromatograph equipped with a mass spectrometer, and the analysis column is ODS-AM (4.6 x 250 mm) manufactured by YMC Co., Ltd., and the mobile phase is a solvent of a mixture of water and acetic acid (1000:5) and acetonitrile in a ratio of 70:30, and the column temperature is 40°C and the flow rate is 0.7 mL / min. Caffeic acid and ferulic acid can be measured by a high performance liquid chromatograph equipped with a mass spectrometer. The analytical column is Imtakt's Unison UK-C18 (3 x 150 mm), and the mobile phase is a mixture of water and acetic acid (1000:5) (mobile phase A) and acetonitrile (mobile phase B). The column temperature is 40°C and the flow rate is 0.7 mL / min. The gradient conditions are as follows. In addition, when measuring, appropriate treatment may be performed as necessary, such as removing impurities in the sample to match the separation ability of the device.

[0035] [Table B]

[0036] The mass ratio of the protein and each polyphenol compound is not particularly limited, but since it exerts an excellent effect of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength, for example, when the composition of the present invention is a solid preparation (powder, granule, tablet, capsule, chewable, etc.), it is preferably 100:0.000001-500, more preferably 100:0.00001-300, and particularly preferably 100:0.001-100. Furthermore, for example, when the composition of the present invention is a liquid preparation (liquid, gel, jelly, paste, etc.), it is preferably 100:0.0000001-100, more preferably 100:0.000001-50, and particularly preferably 100:0.00001-10.

[0037] The mass ratio of the total content of quercetin, caffeic acid and ferulic acid to the content of kaempferol is preferably 0.01 or more, and more preferably 0.1 or more, from the viewpoint of excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength of the obtained food and beverage composition. It is preferably 1 or more, and particularly preferably 1 or more. It is also preferably 600 or less, more preferably 400 or less, and particularly preferably 200 or less.

[0038] The composition for food and drink of the present invention may contain other components in addition to the protein and at least one selected from kaempferol, gallic acid, and proanthocyanidin as a polyphenol compound. Examples of the other components include dietary fiber such as water-soluble dietary fiber and insoluble dietary fiber, minerals other than vitamins and calcium, plants or processed plant products, animals or processed animal products, algae, and microorganisms such as lactic acid bacteria. In addition, polyphenol compounds other than kaempferol, gallic acid, and proanthocyanidin, sugars such as dextrin and starch, oligosaccharides, sweeteners, acidulants, nutritional supplements, stabilizers, lubricants, binders, gloss agents, thickeners, colorants, excipients, diluents, bulking agents, emulsifiers, food additives, and seasonings that are commonly used in the food industry can be mentioned as necessary. The content of these other components can be appropriately selected depending on the form of the composition of the present invention.

[0039] The form of the composition for food and drink of the present invention is not particularly limited and can be any form. Specific forms include, for example, powder such as powder, granules, fine granules, tablets (chewable), spheres, capsules, caplets, liquids, and the like. Capsule-shaped compositions include soft capsules and hard capsules. Specific examples of liquid beverages include containerized beverages filled in PET bottles, cans, bottles, etc., and instant beverages to be dissolved in water (hot water), milk, fruit juice, green juice, etc. The composition of the present invention is preferably in a powder form such as powder, granules, fine granules, or liquid form, and is particularly preferably in a powdered beverage that is mixed with water, etc., dissolved or suspended to be used, which is excellent in stability as a composition and can be easily taken during meals, and unlike tablet or capsule forms, a large amount of the composition can be taken at one time, and the preference can be increased, which is preferable.

[0040] The edible composition of the present invention can be produced by a conventional method. When producing the edible composition of the present invention, the form of the raw material used is not particularly limited, and can be appropriately selected and used according to the form of the composition. For example, when obtaining a powdery or liquid composition such as powder, granules, or fine granules, the protein and the polyphenol compound other than at least one selected from kaempferol, gallic acid, and proanthocyanidin may be used as they are, or may be used as a mixture with an excipient, a bulking agent, or the like. In addition, when obtaining a capsule-shaped edible composition, it may be used that has been dissolved or dispersed in advance in a solvent such as water or edible oil. EXAMPLES

[0041] The present invention will be described below based on examples, but the present invention is not limited to these examples.

[0042] Test 1. Evaluation of mitochondrial activation ability Mitochondria are organelles that play a central role in energy production within cells, and are also essential for muscle activity, development, and maintenance, and it is known that a decrease in their activity leads to rapid muscle atrophy. Therefore, by confirming the mitochondrial activation effect, it is possible to confirm the effect of maintaining and / or increasing muscle mass and the effect of maintaining and / or improving muscle strength. [Test substance] The following substances were used as test substances. Soybean-derived protein: Commercially available powdered soybean protein was used. Whey protein: Commercially available powdered whey protein was used. Kaempferol (WAKO:110-00451) · Quercetin: Quercetin dehydrate (Sigma-Aldrich: Q0125) Caffeic Acid (TCI:C0002) Ferulic Acid: trans-Ferulic Acid (TCI:H0267) ·Gallic Acid (nacalai tesque:16520-42) · Proanthocyanidin: Procyanidin B1 (ChemFaces:CFN99557)

[0043] [Preparation of test substance] Each test substance was prepared using a differentiation-inducing medium (2% HS (horse serum)-DMEM). The compounding ratio of the test substances used in the following tests is shown in Table 1.

[0044] [Mitochondrial activity measurement] (1)37℃, 5vol%CO 2 In the incubator, 75 cm 2 Mouse myoblast cells (C2C12) were cultured in a flask in growth medium (10% FBS-DMEM). (2) The cells were suspended by trypsinization and seeded into each well of a 96-well collagen-coated plate at a cell density of 8 x 10^3 cells / well. The cells were incubated at 37°C and 5% CO 2 The cells were pre-cultured in an incubator for 24 hours. (3) After removing the medium from each well, wash the wells twice with serum-free DMEM, add 200 μL / well of differentiation-inducing medium (2% HS-DMEM), and incubate at 37°C and 5% CO 2 The cells were cultured in an incubator for 6 days. (4) The medium was changed every 2 to 3 days to allow differentiation into myotube cells. (5) After removing the medium from each well, add the medium containing the test substance at 100 μL / well and incubate at 37°C, 5% by volume CO 2 The cells were cultured in an incubator for 24 hours. (6) A differentiation-inducing medium was used as the medium, and the total concentration of the test substances in the medium was 3 μg / mL. The amount of each test substance added (unit: μg / mL) is as shown in Tables 1 to 3. (7) After 24 hours of culture, the medium was removed and each well was washed twice with serum-free DMEM. Then, 50 μL of 10 μM Rhodamine 123 (Fujifilm Wako Pure Chemical Industries, Ltd.) prepared in serum-free DMEM was added to each well. (8)37℃, 5vol%CO 2 After allowing the plate to stand in an incubator for 30 minutes, the reagent was removed and each well was washed twice with PBS. (9) After removing the PBS from each well, 200 μL of PBS was added to the well, and the fluorescence intensity (excitation wavelength Ex. 507 nm, fluorescence wavelength Em. 529 nm) was measured. (10) The percentage of control was calculated based on the data obtained. % of control = (Data sample - Data blank) / (Data control - Data blank) × 100 The relative values, with the control (medium only) value taken as 100, are shown in Tables 1 to 3.

[0045] [Table 1]

[0046] [Table 2]

[0047] [Table 3]

[0048] In Comparative Examples 1 to 6 in which each test substance was added alone, the mitochondrial activity was 91.3 to 98.1% of control, whereas in Examples 1 to 22 in which a protein and a specific polyphenol were contained, the mitochondrial activity was significantly increased to 102.3 to 125.1%. In addition, it was found that when kaempferol contains one or more selected from quercetin, caffeic acid, and ferulic acid, the mitochondrial activity was more significantly increased. From the above, it was found that the composition of the present invention has an excellent mitochondrial activation effect, and therefore has an effect of maintaining and / or increasing muscle mass and an effect of maintaining and / or improving muscle strength. That is, the composition of the present invention is useful as a composition for maintaining and / or increasing muscle mass and a composition for maintaining and / or improving muscle strength.

[0049] <Production Examples 1 to 8> Granules were prepared according to the composition shown in Table 1 below. The composition for food and drink obtained in the following preparation example contains a protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin, or kaempferol plus at least one selected from the group consisting of quercetin, caffeic acid, and ferulic acid. By taking 10 g of the composition once a day with water, the excellent effects of the present invention, such as muscle building and muscle strength maintenance, can be enjoyed. When the obtained composition for food and drink was taken, the taste and odor of the protein were improved, and a composition for food and drink with excellent overall palatability was obtained.

[0050] [Table 4]

[0051] <Production Examples 9 to 16> A powdered beverage was produced according to the composition shown in Table 2 below. The composition for food and drink obtained in the following production example is a mixture of protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin, or kaempferol plus at least one selected from the group consisting of quercetin, caffeic acid, and ferulic acid, and is mixed with 200 mL of water twice a day at 40 g per serving, and consumed to enjoy the excellent effects of the present invention, such as muscle building and muscle maintenance. In addition, when the obtained composition for food and drink was ingested, the taste and odor of the protein were improved, and a composition for food and drink with excellent overall palatability was obtained.

[0052] [Table 5]

[0053] <Production Examples 17 to 24> Protein bars were produced according to the composition shown in Table 3 below. The composition for consumption obtained in the following production example contains a protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin, or kaempferol plus at least one selected from the group consisting of quercetin, caffeic acid, and ferulic acid. By ingesting one bar (40 g) once a day, the excellent effects of the present invention, such as muscle building and muscle strength maintenance, can be enjoyed. Furthermore, when the obtained composition for consumption was ingested, the taste and odor of the protein were improved, and a composition for consumption with excellent overall palatability was obtained.

[0054] [Table 6]

[0055] <Production Example 25 to Production Example 32> A liquid beverage was produced according to the composition shown in Table 4 below. The composition for food and drink obtained in the following production example is a blend of protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin, or kaempferol plus at least one selected from the group consisting of quercetin, caffeic acid, and ferulic acid, and can be taken once a day at 450 ml per serving to enjoy the excellent effects of the present invention, such as muscle building and muscle strength maintenance. Furthermore, when the obtained composition for food and drink was taken, the taste and odor of the protein were improved, and a composition for food and drink with excellent overall palatability was obtained.

[0056] [Table 7] [Industrial Applicability]

[0057] According to the present invention, a food composition having excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength can be obtained by containing a protein and at least one polyphenol compound selected from kaempferol, gallic acid, and proanthocyanidin. Also, according to the present invention, a food composition having excellent effects of maintaining and / or increasing muscle mass and maintaining and / or improving muscle strength can be obtained by containing a protein and a polyphenol compound, kaempferol, and at least one polyphenol compound selected from the group consisting of quercetin, caffeic acid, and ferulic acid.

Claims

[Claim 1] A food or drink composition comprising a protein and at least one polyphenol compound selected from kaempferol, gallic acid and proanthocyanidin.

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