Stinkbug repellent for outdoors and method for using the same

Abstract

To provide a stinkbug repellent which prevents stinkbugs from being attached to crops, and prevents agricultural damages caused by the stinkbugs.SOLUTION: There is provided a stinkbug repellent for outdoors, wherein culture solution is aqueous solution containing culture solution of a bacterial group belonging to at least 14 bacterial genuses, when the total mass of bacterial cells of the bacterial group is 100 mass%, the aqueous solution contains culture solution which is cultured so that the mass of the bacterial cells of the bacterial group becomes 3 to 30 mass% of the mass of the culture solution, a fulvic acid, a pyroligneous acid, and zinc ions as active ingredients.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a repellent for outdoor stink bugs and a method for using the same, and more particularly to an outdoor stink bug repellent that is sprayed on fields and the like to prevent stink bugs from approaching cultivated crops and prevent damage to agricultural products, and a method for using the same.

Background Art

[0002] It is known that there are many types of insects belonging to the order Hemiptera (hereinafter simply referred to as "stink bugs"), and among them, there are those with greatly different properties (see, for example, Non-Patent Document 1). However, stink bugs as a whole have the following common characteristics.

[0003] Many stink bugs are unpleasant pests that emit a strong unpleasant odor such as hexanal or trans-2-hexenal when stimulated. And these malodorous substances that cause these unpleasant odors exhibit properties as warning signal substances, aggregation pheromones, sex pheromones, etc. of stink bugs (see, for example, Non-Patent Document 2). However, since there are many types of stink bugs, different stink bugs generate different malodorous substances, and one type of stink bug may emit multiple malodorous substances, there are a wide variety of malodorous substances, and their details, actions, and essences have not been elucidated.

[0004] In addition, stink bugs have long tubular mouthparts and suck the sap of nutrients from a variety of agricultural products such as beans, solanaceous plants, potatoes, sweet potatoes, rice, tomatoes, and fruits, causing great damage to agricultural products. Moreover, since the bite marks of stink bugs are small, it may not be noticed that stink bugs have attached to the crops, and the damage is likely to spread. Furthermore, if the unpleasant odor of stink bugs adheres to the products, the commercial value of the products may decrease, etc. Stink bugs are pests that cause great damage to agricultural products.

[0005] Stink bugs generally lay eggs in forests, grasslands, farmlands, etc. around April or May and become adults in about a month, so they rapidly multiply from around June. There are also cases where many females are attracted to the sex pheromone released by a single male, and in some cases, they multiply explosively. After that, they are active until autumn and become unpleasant pests that semi-hibernate in winter or move to warm places (such as the corners of houses) and emit a strong unpleasant odor.

[0006] When stink bugs attach to agricultural products, pesticides (insecticides) such as Benika (trade name, Sumitomo Chemical Horticulture Co., Ltd.) are effective. However, using pesticides causes an economic burden, is not environmentally friendly, and there are also agricultural products for which pesticides cannot be used. Moreover, if one tries to forcibly control stink bugs, it may generate a bad smell, and there is a possibility that the value of the product will decrease and it will become unsellable. For this reason, research has been conducted to develop stink bug repellents (see, for example, Patent Documents 1 and 2), but no practical repellent has been found yet.

Prior Art Documents

Patent Documents

[0007]

Patent Document 1

Patent Document 2

Non-Patent Documents

[0008]

Non-Patent Document 1

Non-Patent Document 2

Non-Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0009] An object of the present invention is to provide an outdoor stinkbug repellent and a method for using the same, which prevent damage to crops caused by stinkbugs by spraying a substance repellent to stinkbugs on agricultural land to prevent stinkbugs from attaching to crops.

Means for Solving the Problems

[0010] The outdoor stinkbug repellent of the present invention for solving such problems is obtained by fermenting a fermentable organic substance with a mixed bacterium of bacteria of the following 13 genera and a white rot basidiomycete, and water, and culturing the mixture so that the total mass of the bacterial cells becomes 3 to 30% by mass of the total mass to decompose the organic substance, and then mixing a culture solution, fulvic acid, wood vinegar, zinc ions, and water. The mixture is characterized in that: When the total mass of the mixture is 100% by mass, the mass of the culture solution is 5 to 15% by mass, the mass of fulvic acid is 0.0001 to 0.01% by mass, the mass of wood vinegar is 0.2% to 10% by mass, and 0.01 to 10.0 millimoles of zinc ions are contained per 1 kg of the total weight of the mixture. (1) Streptomyces (2) Sulfolobus (3) Saccharopolyspora (4) Nocardia (5) Pseudomonas (6) Bacillus (7) Cytophaga (8) Candida (9) Cellulomonas (10) Clostridium (11) Aspergillus (12) Penicillium (13) Rhizopus

[0011] In addition, the method for using the outdoor stinkbug repellent of the present invention is characterized by diluting the outdoor stinkbug repellent 20 to 50 times with water and spraying it.

Effect of the Invention

[0012] The stinkbug repellent of the present invention can disperse stinkbugs from cultivated crops by spraying it on the crops, and can prevent damage to agricultural products caused by stinkbugs.

Mode for Carrying Out the Invention

[0013] The outdoor stinkbug repellent of the present invention is a mixed solution obtained by mixing fulvic acid, wood vinegar, zinc ions, and water with a culture solution obtained by fermenting and culturing fermentable organic substances.

[0014] The organic substance used as a raw material is not particularly limited as long as it is an organic substance capable of being fermented and decomposed. Preferred examples include, for example, waste from agricultural product processing, waste from fishery product processing, food waste, etc., but are not limited thereto.

[0015] [Regarding the bacteria used for culturing] The bacteria used in the production of the stink bug repellent of the present invention consist of bacteria belonging to the following 13 genera of bacteria and white-rot basidiomycetes, and the genera are: (1) Streptomyces, (2) Sulfurovum, (3) Saccharopolyspora, (4) Nocardia, (5) Pseudomonas, (6) Bacillus, (7) Cytophaga, (8) Candida, (9) Cellulomonas, (10) Clostridium, (11) Aspergillus, (12) Penicillium, and (13) Sporothrix.

[0016] The bacteria used in the preparation of the culture solution need to include all of the bacteria belonging to the above 13 genera and all of the white-rot basidiomycetes. By including all of the bacteria belonging to the 13 genera and all of the white-rot basidiomycetes, the decomposition and fermentation actions between the bacteria can be increased.

[0017] [Regarding the culture solution] The culture solution of the present invention is prepared by mixing the bacteria belonging to the above 13 genera and white-rot basidiomycetes, adding an organic substance fermentable and decomposable in the culture medium and water, and culturing them. These bacteria are preferably cultured so that the total mass of the bacterial cells becomes 3 to 30% by mass based on the total mass of the culture solution. If the mass of the bacterial cells is less than 3% by mass based on the mass of the culture solution, a sufficient repellent effect against stink bugs cannot be obtained when used in the stink bug repellent. On the other hand, culturing to exceed 30% by mass is often difficult, and the activity of the bacteria may decrease or change, so it is not preferable.

[0018] After the completion of the culture, the culture solution is filtered using a filter medium with a pore size through which the bacterial cells can pass to remove the residues of the culture, the generated precipitates, dust, etc., to obtain a culture solution containing the bacterial cells. Here, the water-soluble heavy metal components containing one or more of cadmium, mercury, and arsenic contained in the organic substance change to water-insoluble solids and precipitate during the culture. Therefore, when the solids are filtered off, the heavy metal components become water-insoluble and are removed.

[0019] [Regarding fulvic acid] Fulvic acid is a substance that gives a yellow to orange-yellow color to an acidic supernatant when extracted with an inorganic acid after extracting and removing humic acid from soil or coal with a dilute alkali, and gives an amorphous powder when dried. The obtained powder is an amorphous acidic substance soluble in water and ethanol. Fulvic acid does not have a single chemical structural formula, nor is its composition or molecular weight constant. Its composition and molecular weight vary depending on the raw materials and extraction conditions. Also, in practical applications, those supplied as aqueous solutions are often used. In the present invention, it is preferable to use purified fulvic acid produced by the method described in Non-Patent Document 3.

[0020] [Regarding wood vinegar] Wood vinegar is a pyrolysis liquid obtained as a distillate when wood is carbonized, and it has been reported to have a weak repellent effect against stink bugs (see Patent Document 2). However, wood vinegar is expensive because only a small amount can be obtained even when a large amount of wood is carbonized, and its stink bug repellent effect is also weak. Therefore, although it can be used for home gardening, it cannot be used for agriculture. In contrast, in the present invention, by adding a small amount of wood vinegar to a culture solution of bacteria belonging to 13 genera and white rot basidiomycetes, the repellent effect against stink bugs can be significantly enhanced.

[0021] [Regarding zinc ions] Among filamentous fungi in a relationship of cytoplasmic incompatibility, even when co-cultured, the transmission (transfer) of mycophagous viruses does not occur. However, when co-cultured in the presence of zinc ions, the virus migrates (transmits) from the virus-infected mycophagous virus to the filamentous fungi not infected with the mycophagous virus. Therefore, zinc ions are used. In this way, the repellent power of stink bugs is increased by the migration of the virus by zinc ions.

[0022] [Composition of stink bug repellent] The stink bug repellent of the present invention is a mixed solution obtained by mixing the above culture solution, fulvic acid, wood vinegar, zinc ions, and water. When the mass of the entire above-mentioned mixed solution is 100% by mass, the mass of the culture solution is preferably 5% to 15% by mass. When the mass of the culture solution is less than 5% by mass, the repellent effect of the stink bug repellent decreases. Even if the mass of the culture solution is added in excess of 15% by mass, compared to the increase in the amount of the added culture solution, the increase in the repellent effect of the stink bug repellent does not correspond, which is not preferable.

[0023] Also, when the mass of the entire above-mentioned mixed solution is 100% by mass, the mass of fulvic acid in the stink bug repellent of the present invention is preferably 0.0001% to 0.01% by mass. When the mass of fulvic acid is less than 0.0001% by mass, the repellent effect of the stink bug repellent decreases. Even if the mass of fulvic acid is added in excess of 0.01% by mass, the increase in the repellent effect of the stink bug repellent does not correspond to the increase in the amount of the added fulvic acid, which is not economically preferable.

[0024] Furthermore, when the mass of the entire mixture is 100% by mass, the content of wood vinegar is preferably 0.2% to 10.0% by mass. When the mass of wood vinegar is less than 0.2% by mass, the repellent effect of the stink bug repellent decreases. Even if the mass of wood vinegar is added in excess of 10.0% by mass, the increase in the repellent effect of the stink bug repellent does not correspond to the increase in the amount of the added component, which is not economically preferable.

[0025] Furthermore, the content concentration of zinc ions is preferably 0.1 to 10.0 mmol concentration / stink bug repellent. When the content of zinc ions is less than 0.1 mmol concentration, the repellent effect of the stink bug repellent decreases. Even if the content of the zinc ions is added so as to exceed 10 mmol, the repellent effect of the stink bug repellent does not increase, which is not economically preferable.

[0026] [Examples] (1) Culture solution As the mixed bacteria of the 13 genera of bacteria and the white-rot basidiomycete of the present invention, the following 14 bacterial species can be mentioned. The attached symbols correspond to the above 13 bacterial families. 1) Streptomycin-producing bacterium (Streptomyces griseus genus Streptomyces) 2) Sulfolobus acidocaldarius (Sulfolobus acidoca ldarius, genus Sulfolobus) 3) Saccharopolyspora erythraea (Saccharopolyspora erythraea, genus Saccharopolyspora) 4) Nocardia asteroides (Nocardia asteroides, genus Nocardia) 5) Pseudomonas fluorescens (Pseudomonas fluores cens, genus Pseudomonas) 6) Bacillus subtilis (Bacillus subtilis, genus Bacillus) 7) Cytophaga hutchinsoni (Cytophaga hutchinsoni , genus Cytophaga) 8) Candida versatilis (Candida versatilis, genus Candida ) 9) Cellulomonas flavigena (Cellulomonas flavigena , genus Cellulomonas) 10) Clostridium thermocellum (Clostridium thermoce llum, genus Clostridium) 11) Aspergillus oryzae (Aspergillus oryzae, genus Asper gillus) 12) Penicillium chrysogenum (Penicillium chrysogenu m, genus Penicillium) 13) Rhizopus oryzae (Rhizopus oryzae, genus Rhizopus) In the present invention, as the white-rot basidiomycete, a white-rot fungus is used.

[0027] Each of the 13 genera of bacteria and the white-rot basidiomycete was mixed in the same amount at a concentration of 1×10 5 cfu / cm 3 and pre-cultured. The pre-culture solution was cultured until the total mass of the cells reached 10% by mass to produce a culture solution of the mixed bacteria. This culture solution was filtered using a filter medium with a pore size through which the cells could pass to obtain a culture solution.

[0028] (2) Wood vinegar 150 Kg of raw logs (cedar, beech, cherry, and oak wood) were charged into a wood distillation apparatus, heated to a temperature of 300 °C or higher in the kettle, the distillate was collected and allowed to stand, and 20 kg of wood vinegar was obtained as the supernatant after removing the tar that settled.

[0029] (4) Stink bug repellent 10 kg of the culture solution, 5.0 kg of fulvic acid produced according to the method described in Patent Document 3, 5.0 kg of wood vinegar, and 2.76 g (0.02 mol) of zinc chloride were added with water to produce 200 kg of the stink bug repellent of the present invention. In this case, the water used for the production of the stink bug repellent is preferably water that does not contain an oxidizing agent.

[0030] In the present invention, the stink bug repellent produced as described above is diluted 20 to 50 times with water and then sprayed on agricultural land for use as a stink bug repellent. When the dilution ratio is less than 20 times, handling becomes difficult due to the high concentration. When the dilution ratio exceeds 50 times, a sufficient repellent effect cannot be exhibited.

[0031] [Test Example] (1) Regarding the test apparatus Two plastic boxes in the shape of cubes with one side being 30 cm were arranged in a row with a cylindrical box with a diameter of 7 cm sandwiched in between, and they were connected and made to communicate with a pipe with a diameter of 1 cm and a length of 30 cm. A glass petri dish with a diameter of 9 cm covered with filter paper with a diameter of 8.5 cm was placed in the cylindrical box. 0.5 mL of the sample was dropped onto the filter paper on one side to serve as the sample side, and the same amount of water was dropped onto the other side to serve as the control side. Then, 10 small green stink bugs were put into the central cylindrical box, and after breeding them for 3 days under the conditions of 12 hours of light and 12 hours of darkness at room temperature while supplying food and water, the number of moving stink bugs was examined. The number of moving stink bugs was divided into three types: the sample side box, the control side box, and other locations, and the number of stink bugs present in each was counted. The test was repeated 10 times. The movement of stink bugs from the central stink bug input box to the sample side was judged to be an attracting effect of the sample on the stink bugs, and the movement to the control side was judged to be a repellent effect of the plant pyrolysis liquid on the stink bugs. The samples used for the measurement are shown below, and the test results are shown in Table 1. Note that the sample containing no zinc ions was excluded from the test because it did not show a repellent effect on stink bugs in the preliminary test.

[0032] (2) Regarding the samples used for the measurement Sample No. 1: Stink bug repellent described in the example 10 parts by mass of the culture solution, 0.005 parts by mass of fulvic acid, 5 parts by mass of wood vinegar solution, 2.76 g of zinc chloride and water were added to make 100 parts by mass. Sample No. 2: 10 parts by mass of the culture solution, 0.005 parts by mass of fulvic acid, 2.76 g of zinc chloride and water were added to make 100 parts by mass. This sample is used as a disinfectant for apple rot disease. Sample No. 3: 10 parts by mass of the culture solution, 10 parts by mass of wood vinegar solution, 2.76 g of zinc chloride, and water were added to make 100 parts by mass. Sample No. 4: 10 parts by mass of wood vinegar solution, 2.76 g of zinc chloride, and water were added to make 100 parts by mass thereof. Sample No. 5: 10 parts by mass of the culture solution, 0.005 parts by mass of fulvic acid, and 2.76 g of zinc chloride and water were added to make 100 parts by mass. Sample No. 6: water

[0033] (3) [Test Results]

Table 1

[0034] As shown in Table 1, the stinkbug repellent of the present invention (Sample No. 1) showed a stronger repellent effect against stinkbugs than the apple rot disinfectant (Sample No. 2) and the wood vinegar solution (Sample No. 4). The mixed solution of the culture solution and the wood vinegar solution (Sample No. 3) also showed a strong repellent effect against stinkbugs, but it was not as good as the stinkbug repellent of the present invention (Sample No. 1). The mixed solution of the wood vinegar solution and fulvic acid (Sample No. 5) showed only a repellent effect against stinkbugs equivalent to that of fulvic acid (Sample No. 4).

Claims

1. A mixture of a culture liquid obtained by fermenting fermentable decomposable organic matter using a mixture of bacteria belonging to the following 13 species of fungus and a white-rot basidiomycete fungus and culturing the mixture so that the total mass of the fungus accounts for 3 to 30% by mass of the total mass, decomposing the organic matter, a fulvic acid, wood vinegar, zinc ions, and water, When the total mass of the mixed solution is 100% by mass, the mass of the culture solution is 5% by mass to 15% by mass, the mass of the fulvic acid is 0.0001% by mass to 0.01% by mass, and the mass of the wood vinegar is 0.2% by mass to 10% by mass; An outdoor stink bug repellent comprising 0.01 to 10.0 millimoles of zinc ions per 1 kg of the total weight of the mixture. (1) Streptomyces (2) Sulfolobus (3) Saccharopolyspora (4) Nocardia (5) Pseudomonas (6) Bacillus (7) Cytophaga (Cytophaga) (8) Candida (9) Cellulomonas (10) Clostridium (11) Aspergillus (12) Penicillium (13) Rhizopus

2. 2. A method for using the outdoor stink bug repellent according to claim 1, comprising diluting the stink bug repellent with water 20 to 50 times and spraying the diluted stink bug repellent.

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