Biosensor strip for specimen measurement, electrode structure thereof, and method for specimen measurement using the same

Abstract

To provide a biosensor strip for a specimen measurement, an electrode structure thereof, and a method for specimen measurement using the electrode structure.SOLUTION: The electrode structure of the biosensor strip according to one embodiment includes: a strip recognition electrode 131 used for recognizing that the biosensor strip has been inserted into a specimen measurement device; a specimen recognition electrode 132 used for recognizing a specimen; and a working electrode 134 and a working reference electrode 135 used for measuring the specimen.SELECTED DRAWING: Figure 4

Description

[Technical Field]

[0001] The following embodiments relate to a biosensor strip for measuring an analyte, its electrode structure, and an analyte measurement method using the same. [Background technology]

[0002] Quantitative and qualitative analysis of analytes present in biological samples is an important task both in chemistry and clinical practice, such as measuring blood glucose levels in diabetic patients and cholesterol levels, which are factors contributing to adult diseases.

[0003] As is well known in the art, it is extremely important for electrochemical biosensors that utilize enzyme activity to rapidly and reproducibly measure the enzyme activity of biological samples (hereinafter referred to as "analytes"), such as specific substances in saliva or blood, glucose, uric acid, protein, DNA, and sucrose in clinical chemistry tests, and GOT (Glutamate-Oxaloacetate Transaminase) or GPT (Glutamate-Pyruvate Transaminase) in liver function tests. Here, the biosensor is composed of an identification site that identifies the measurement target and a change site that converts the activity into an electrical signal.

[0004] A biological substance is used in the recognition site, and when the biological substance recognizes the target, a chemical or physical change occurs. The site that converts this change into an electrical signal is the change site, and the recognition site and change site are collectively called the biosensor electrode.

[0005] The measurement method of strip-type biosensors currently on the market is generally to use capillary action, a force stronger than gravity made possible by plasma or chemical surfactant treatment during the manufacturing process, to bring the sample into contact with and flow into the sample insertion channel of the biosensor, allowing the sample to accumulate in the sample insertion channel, and then apply a sample measurement signal to the working electrode and working reference electrode to measure the response signal to the sample.

[0006] In conventional biosensors, in order to determine whether a sample has come into contact with the biosensor strip when measuring the sample, the time point of sample contact and inflow is determined by applying a sample recognition signal to the working electrode and working reference electrode.

[0007] However, in such cases, because the working electrode and working reference electrode used in the analyte measurement process are also used in the process of recognizing the contact of the analyte, an electric double layer (EDL) may be formed on the surfaces of the working electrode and working reference electrode before the analyte is measured. The electric double layer appears when an electric field is applied at the boundary between different materials (electrode, analyte, or solution). Even if the amount of electric double layer capacitance (DLC) is small, it can be included in the response current signal for the analyte measured when an analyte measurement signal is applied. Therefore, conventional techniques that form an electric double layer on the working electrode and working reference electrode before the analyte measurement have the problem of reduced accuracy in the analyte measurement.

[0008] This has led to a demand for proposals for technologies that can solve the problems that conventional biosensors have. Summary of the Invention [Problem to be solved by the invention]

[0009] One embodiment proposes an electrode structure of a biosensor strip that includes a strip recognition electrode and an analyte recognition electrode that are individually provided to operate electrically and separately with the working electrode and the working reference electrode to prevent the formation of an electrical double layer on the surfaces of the working electrode and the working reference electrode before the analyte measurement, thereby improving the accuracy of the analyte measurement.

[0010] Furthermore, one embodiment proposes an electrode structure of a biosensor strip in which the strip recognition electrode and the sample recognition electrode each share a recognition reference electrode in order to reduce the size of the biosensor strip.

[0011] In addition, one embodiment proposes an electrode structure of a biosensor strip that includes a dummy electrode for contacting the sample with the sample insertion channel more widely than a predetermined size in order to improve poor spray diffusion due to interface material properties. However, the technical problem to be solved by the present invention should not be limited to the above, and may be variously expanded within the scope that does not deviate from the technical idea and scope of the present invention. [Means for solving the problem]

[0012] According to one embodiment, the electrode structure of a biosensor strip for analyte measurement may include a strip recognition electrode used to recognize the insertion of the biosensor strip into an analyte measurement device, an analyte recognition electrode used to recognize the analyte, and a working electrode and a working reference electrode used to measure the analyte.

[0013] According to one aspect, the strip recognition electrode and the analyte recognition electrode may be provided separately so as to operate electrically separately from the working electrode and the working reference electrode.

[0014] According to another aspect, the working protrusion formed in the working electrode in a region corresponding to the sample insertion channel of the biosensor strip and the at least two working reference protrusions formed in the working reference electrode in a region corresponding to the sample insertion channel may be arranged alternately while protruding in a direction perpendicular to the direction in which the working electrode and the working reference electrode are extended.

[0015] According to another aspect, a recognition reference electrode for each of the strip recognition electrode and the analyte recognition electrode may be provided in common with the strip recognition electrode and the analyte recognition electrode.

[0016] According to another aspect, the analyte recognition protrusion formed in the analyte recognition electrode in a region corresponding to the analyte insertion channel of the biosensor strip and the recognition reference protrusion formed in the recognition reference electrode in a region corresponding to the analyte insertion channel of the biosensor strip may be arranged opposite each other while protruding in a direction perpendicular to the direction in which the analyte recognition electrode and the recognition reference electrode are extended.

[0017] According to another aspect, the electrode structure of the biosensor strip may further include a dummy electrode provided on one side of the sample insertion channel of the biosensor strip.

[0018] According to yet another aspect, the strip recognition electrode, the analyte recognition electrode, the working electrode, and the working reference electrode may be formed to extend in one direction while being spaced apart and parallel to each other.

[0019] According to one embodiment, a biosensor strip for measuring an analyte may include a lower plate having an electrode structure including a strip recognition electrode used to recognize that the biosensor strip has been inserted into an analyte measurement device, an analyte recognition electrode used to recognize the analyte, a working electrode and a working reference electrode used to measure the analyte, a middle plate disposed on top of the lower plate and including a sample insertion flow path into which an enzyme compound that reacts with the analyte is inserted, and an upper plate disposed on top of the middle plate and including an insertion port for inserting the analyte into the sample insertion flow path.

[0020] According to one aspect, the strip recognition electrode and the analyte recognition electrode on the lower plate may be provided separately so as to operate electrically and separately from the working electrode and the working reference electrode.

[0021] According to another aspect, a recognition reference electrode for each of the strip recognition electrode and the analyte recognition electrode on the lower plate may be provided in common with the strip recognition electrode and the analyte recognition electrode.

[0022] In another aspect, the lower plate may further include a dummy electrode provided on one side of the sample insertion channel of the biosensor strip.

[0023] According to one embodiment, the analyte measurement system may include a biosensor strip having an electrode structure including a strip recognition electrode used to recognize the insertion of the biosensor strip into an analyte measurement device, an analyte recognition electrode used to recognize the analyte, a working electrode and a working reference electrode used to measure the analyte, and the analyte measurement device that recognizes whether the biosensor strip has been inserted, recognizes whether an analyte has contacted the biosensor strip if the biosensor strip has been inserted into the analyte measurement device, and applies an analyte measurement signal to the working electrode and the working reference electrode to measure a response signal for the analyte if the analyte has contacted the biosensor strip.

[0024] According to one embodiment, an analyte measurement method performed by an analyte measurement system including a biosensor strip and an analyte measurement device may include the steps of: recognizing whether the analyte measurement device is inserted into the biosensor strip using a strip recognition electrode included in the biosensor strip; when the biosensor strip is inserted into the analyte measurement device, recognizing whether a analyte has come into contact with the biosensor strip using the analyte recognition electrode included in the biosensor strip; and when the analyte has come into contact with the biosensor strip, applying an analyte measurement signal to a working electrode and a working reference electrode included in the biosensor strip to measure a response signal for the analyte.

[0025] According to one aspect, the step of measuring the response signal to the analyte may be characterized in that the strip recognition electrode and the analyte recognition electrode are provided separately to operate electrically and independently from the working electrode and the working reference electrode, and thus the step of recognizing whether the biosensor strip has been inserted into the analyte measurement device and the step of recognizing whether the analyte has contacted the biosensor strip may be performed independently from the steps of recognizing whether the strip recognition electrode and the analyte recognition electrode have been provided separately to operate electrically and independently from the working electrode and the working reference electrode.

[0026] According to another aspect, the sample measurement method may further include contacting the sample with a sample insertion channel of the biosensor strip wider than a predetermined size using a dummy electrode provided on one side of the sample insertion channel of the biosensor strip. [Effects of the Invention]

[0027] One embodiment proposes an electrode structure of a biosensor strip including a strip recognition electrode and an analyte recognition electrode that are individually provided to operate electrically and separately with the working electrode and the working reference electrode, thereby achieving the technical effect of preventing the formation of an electrical double layer on the surfaces of the working electrode and the working reference electrode before the analyte measurement, thereby improving the accuracy of the analyte measurement.

[0028] Furthermore, one embodiment proposes an electrode structure of a biosensor strip in which the strip recognition electrode and the sample recognition electrode each have a common recognition reference electrode, thereby achieving the technical effect of miniaturizing the biosensor strip.

[0029] Furthermore, one embodiment proposes an electrode structure of a biosensor strip that includes a dummy electrode for contacting the sample with the sample insertion channel wider than a predetermined size, thereby achieving the technical effect of improving poor dispensing diffusivity due to interface material properties.

[0030] However, the effects of the present invention should not be limited to those described above, and may be variously expanded within the scope of the technical idea and scope of the present invention. [Brief explanation of the drawings]

[0031] [Figure 1] FIG. 1 illustrates an analyte measurement system in one embodiment. [Figure 2] FIG. 2 is a diagram showing the specimen collection device shown in FIG. 1. [Figure 3] FIG. 2 is a diagram showing the biosensor strip shown in FIG. 1. [Figure 4] 4 is a diagram showing the electrode structure of the lower plate of the biosensor strip shown in FIG. 3. FIG. [Figure 5] 1 is a flowchart illustrating a sample measurement method in one embodiment. [Figure 6] FIG. 6 is a diagram showing an example for explaining the sample measurement method shown in FIG. 5. DETAILED DESCRIPTION OF THE INVENTION

[0032] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention should not be limited to or constrained by such embodiments. The same reference numerals shown in the drawings denote the same elements.

[0033] Furthermore, the terminology used herein is intended to appropriately describe preferred embodiments of the present invention, but may vary depending on the intentions of viewers and operators, the practices of the field to which the present invention pertains, and other factors. Therefore, definitions of such terms should be based on the overall content of this specification. For example, the singular forms used herein also include the plural forms unless otherwise specified in the context. Furthermore, the terms "comprises" and / or "comprising" used herein do not imply that a referenced component, step, action, and / or element excludes the presence or addition of one or more other components, steps, actions, and / or elements. Furthermore, although terms such as "first" and "second" are used herein to describe various regions, directions, shapes, and the like, these regions, directions, and shapes should not be construed as being limited by such terms. These terms are merely used to distinguish a given region, direction, or shape from other regions, directions, or shapes. Therefore, a portion referred to as a "first portion" in one embodiment may be referred to as a "second portion" in another embodiment.

[0034] Furthermore, it should be understood that the various embodiments of the present invention, although different from one another, are not necessarily mutually exclusive. For example, a particular shape, structure, and characteristic described in this specification may be implemented in other embodiments without departing from the spirit and scope of the present invention. It should also be understood that the location, arrangement, or configuration of individual components within the scope of each presented embodiment can be changed without departing from the spirit and scope of the present invention.

[0035] Hereinafter, a biosensor strip according to an embodiment, its electrode structure, a sample measurement system including the same, and a sample measurement method using the same will be described with reference to the drawings.

[0036] Figure 1 is a diagram showing a sample measurement system according to one embodiment, Figure 2 is a diagram showing the sample collection device shown in Figure 1, Figure 3 is a diagram showing the biosensor strip shown in Figure 1, and Figure 4 is a diagram showing the electrode structure of the lower plate of the biosensor strip shown in Figure 3.

[0037] An analyte measurement system 100 according to one embodiment may include an analyte measurement device 110 , a biosensor strip 120 , and a sample collection device 160 .

[0038] As shown in FIG. 2, the specimen collection device 160 includes a specimen collection portion 161 including a specimen collection swab 161-1 for collecting a specimen, a filter 162, and a compression tube 163.

[0039] In this case, if the sample is saliva, the sample collection unit 161 may collect the saliva through the sample collection swab 161-1, and the saliva collected by the sample collection unit 161 may have interfering substances contained therein removed through a filter 162 inserted into the compression tube 163, and then be provided to the outside, i.e., the biosensor strip 120, through the compression tube 163.

[0040] That is, after collecting saliva, the specimen collection device 160 compresses the specimen collection section 161 in the direction of the compression tube 163 within the compression tube 163, and can remove interfering substances contained in the saliva through the filter 162.

[0041] The filter 162 may be composed of at least one layer. In particular, the thickness or number of layers of the filter 162 may be adjusted depending on the degree of removal of interfering substances to be removed from the sample, and the filtering degree may be adjusted depending on the size of the interfering substances to be removed from the sample. For example, if large molecules and foreign substances present in saliva are to be removed as interfering substances, the filter 162 may be formed to have a filtering degree that is sufficient to remove the large molecules and foreign substances.

[0042] When biosensor strip 120 is inserted into and contacts a sample collected by sample collecting device 160 through sample collecting device 160, it performs the function of sensing the information to be measured (e.g., glucose) from the sample from which interfering substances have been removed. When inserted into sample measuring device 110, biosensor strip 120 may provide sample measuring device 110 with a response signal responsive to a strip recognition signal received from sample measuring device 110, a response signal responsive to a sample recognition signal received from sample measuring device 110, and a response signal responsive to a sample measurement signal received from sample measuring device 110. Thus, sample measuring device 110 can recognize the insertion of biosensor strip 120 and contact with the sample based on the response signal received from biosensor strip 120, and measure the sample.

[0043] Such a biosensor strip 120 may be composed of a bottom plate 130, a middle plate 140, and a top plate 150, as shown in Fig. 3. More specifically, as shown in Fig. 4, the biosensor strip 120 may include a bottom plate 130 on which an electrode structure is formed, a middle plate 140 disposed on the top of the bottom plate 130 and including a sample insertion channel 141 into which an enzyme compound that reacts with a sample is inserted, and a top plate 150 disposed on the top of the middle plate 140 and including an insertion port 151 for inserting a sample into the sample insertion channel 141 and an outlet port 152 for discharging air.

[0044] Here, the specimen insertion channel 141 is obvious to those skilled in the art, so a detailed description thereof will be omitted.

[0045] The enzyme compound 142 may include an enzyme for selectively reacting with glucose contained in the sample, a polymer for attaching the enzyme to an electrode disposed on the lower plate 130, and a catalyst for promoting the reaction between the enzyme and glucose.

[0046] In this case, the catalyst may contain 1 to 10% of at least one of ferrocene, a ferrocene derivative, quinone, a quinone derivative, hexaamine ruthenium (III) chloride, Prussian blue, and a ferricyanide (when Prussian blue is used, the catalyst may contain 0.001 to 5% by weight of Prussian blue), the enzyme may contain 0.001 to 5% by weight of at least one of glucose oxidase and glucose dehydrogenase (GDH), and the polymer may contain 1 to 30 units of at least one of chitosan, PVP, Nafion, polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, agarose, and trehalose.

[0047] The lower plate 130 may have an electrode structure formed thereon, including a strip recognition electrode 131, an analyte recognition electrode 132, a recognition reference electrode 133, a working electrode 134, a working reference electrode 135, a dummy electrode 136, and spare electrodes 137 and 138.

[0048] Strip recognition electrode 131 may be used to recognize whether biosensor strip 120 has been inserted into analyte measurement device 110. As an example, a strip recognition signal may be applied from analyte measurement device 110 via strip recognition electrode 131 and recognition reference electrode 133, and a response signal may be provided from biosensor strip 120 to analyte measurement device 110 via strip recognition electrode 131 and recognition reference electrode 133. Thus, analyte measurement device 110 can recognize whether biosensor strip 120 has been inserted into analyte measurement device 110 using strip recognition electrode 131. Whether or not biosensor strip 120 has been inserted into analyte measurement device 110 may be determined based on the strength of a response signal in response to the strip recognition signal, or the occurrence and presentation of the response signal.

[0049] The analyte recognition electrodes 132 may be used to recognize an analyte. More specifically, the analyte recognition electrodes 132 may be used to recognize whether an analyte has come into contact with the biosensor strip 120. For example, an analyte recognition signal may be applied from the analyte measurement device 110 via the analyte recognition electrodes 132 and the recognition reference electrode 133, and a response signal in response to the analyte recognition signal may be provided from the biosensor strip 120 to the analyte measurement device 110 via the analyte recognition electrodes 132 and the recognition reference electrode 133. Thus, the analyte measurement device 110 can use the analyte recognition electrodes 132 to recognize whether an analyte has come into contact with the biosensor strip 120. Whether an analyte has come into contact with the biosensor strip 120 may be determined based on the strength of the response signal in response to the analyte recognition signal or the occurrence and provision of the response signal.

[0050] The sample recognition protrusion 132-1 formed in the area of ​​the sample recognition electrode 132 corresponding to the sample insertion flow path 141 of the biosensor strip 120 and the recognition reference protrusion 133-1 formed in the area of ​​the recognition reference electrode 133 corresponding to the sample insertion flow path 141 of the biosensor strip 120 may be arranged opposite each other at a distance from each other, protruding in a direction perpendicular to the direction in which the sample recognition electrode 132 and the recognition reference electrode 133 are extended.

[0051] In this case, the recognition reference electrode 133 for each of the strip recognition electrode 131 and the analyte recognition electrode 132 may be provided in common to the strip recognition electrode 131 and the analyte recognition electrode 132. That is, the strip recognition electrode 131 and the analyte recognition electrode 132 may share one recognition reference electrode 133. This allows for miniaturization of the biosensor strip 120, unlike when the strip recognition electrode 131 and the analyte recognition electrode 132 each have their own recognition reference electrode.

[0052] The working electrode 134 and the working reference electrode 135 may be used to measure the analyte. Specifically, an analyte measurement signal may be applied from the analyte measurement device 110 via the working electrode 134 and the working reference electrode 135, and a response signal for the analyte may be provided from the biosensor strip 120 to the analyte measurement device 110 via the working electrode 134 and the working reference electrode 135. Thus, the analyte measurement device 110 can measure the analyte by measuring the response signal for the analyte using the working electrode 134 and the working reference electrode 135. The analyte measurement result may be determined by the strength of the response signal in response to the analyte measurement signal or the occurrence and provision of the response signal. For example, the analyte measurement result may be indicated by a measurement value in multiple stages depending on the strength of the response signal in response to the analyte measurement signal.

[0053] The working protrusion 134-1 formed in the region of the working electrode 134 corresponding to the sample insertion channel 141 of the biosensor strip 120 and two or more working reference protrusions 135-1, 135-2 formed in the region of the working reference electrode 135 corresponding to the sample insertion channel 141 may be arranged alternately while protruding in a direction perpendicular to the direction in which the working electrode 134 and the working reference electrode 135 are extended.

[0054] In this way, one action protrusion 134-1 is arranged between two or more action reference protrusions 135-1, 135-2 and is arranged alternately with each other, and the area of ​​one action protrusion 134-1 is smaller than the area of ​​each of the two or more action reference protrusions 135-1, 135-2, so that when a sample comes into contact with the action protrusion 134-1, the sample measurement signal and the response signal responsive to the sample measurement signal can be accurately measured, thereby improving the accuracy of the sample measurement.

[0055] The strip recognition electrode 131, the sample recognition electrode 132, the recognition reference electrode 133, the working electrode 134, and the working reference electrode 135 may be formed to extend in one direction while being spaced apart from each other in parallel.

[0056] In the above-described electrode structure, the strip recognition electrode 131 and the analyte recognition electrode 132 may be provided separately to operate electrically and separately with the working electrode 134 and the working reference electrode 135. Therefore, the accuracy of the analyte measurement can be improved by preventing the formation of an electrical double layer on the surfaces of the working electrode 134 and the working reference electrode 135 before the analyte measurement.

[0057] The dummy electrode 136 is intended to allow the sample to contact the sample insertion channel 141 more widely than a predetermined size in order to improve poor dispensing diffusibility due to the interface material characteristics, and may be provided on one side of the area corresponding to the sample insertion channel 141 on the lower plate 130.

[0058] The spare electrodes 137 and 138 are spare electrodes in the electrode structure of the biosensor strip 120 described above, and may be used as any one of the strip recognition electrode 131, sample recognition electrode 132, recognition reference electrode 133, working electrode 134, and working reference electrode 135, as needed.

[0059] Although not shown in the drawings, the lower plate 130 on which the above-described electrode structure is formed may be formed by stacking multiple metal layers. As an example, the lower plate 130 may be realized as a four-layer structure including an insulating substrate layer (not shown), a first metal layer (not shown) formed of copper on the insulating substrate layer, a second metal layer (not shown) formed of nickel on the first metal layer, and a third metal layer (not shown) formed of gold on the second metal layer. In this case, the above-described electrode structure may be formed on each of the first metal layer, the second metal layer, the third metal layer, and the fourth metal layer.

[0060] When the biosensor strip 120 into which the sample has come into contact and flowed is inserted, the sample measurement device 110 sequentially applies a strip recognition signal, a sample recognition signal, and a sample measurement signal to the biosensor strip 120, and by receiving response signals to the strip recognition signal, the sample recognition signal, and the sample measurement signal, respectively, it recognizes that the biosensor strip 120 has been inserted and that the sample has come into contact with the biosensor strip 120 and measures the sample.

[0061] The sample measurement results may be provided as multiple levels of measurement values ​​depending on the strength of the response signal in response to the sample measurement signal, and different graphic effects may be applied depending on the measurement values ​​and displayed through the display means of the sample measurement device 110.

[0062] Figure 5 is a flowchart showing a sample measurement method according to one embodiment, and Figure 6 is a diagram showing an example for explaining the sample measurement method shown in Figure 5. The sample measurement method described below is premised on being performed mainly by the sample measurement system 100 described with reference to Figures 1 to 4.

[0063] Prior to steps 510 to 530, the specimen measurement system 100 (more precisely, the specimen collection device 160) may collect saliva from the subject using the specimen collection section 161 of the specimen collection device 160, as shown in Figures 6(a) and 6(b), and remove interfering substances from the specimen, which is saliva, by inserting the collected saliva into a compression tube 163 equipped with a filter 162. Next, the biosensor strip 120 may be inserted into the specimen measurement device 110 as shown in Figure 6(c), and the specimen may be inserted and flown from the specimen collection device 160 into the insertion port 151 of the biosensor strip 120 as shown in Figure 6(d), thereby bringing the specimen into contact with the specimen insertion channel 141 of the biosensor strip 120.

[0064] At this time, the sample inserted and flowing into the biosensor strip 120 can be brought into contact with the sample insertion flow path 141 of the biosensor strip 120 over a wider area than a predetermined size by the dummy electrode 136 provided on one side of the sample insertion flow path 141 of the biosensor strip 120.

[0065] In step 510, the analyte measurement system 100 (more precisely, the analyte measurement device 110) may use the strip recognition electrodes 131 included in the biosensor strip 120 to recognize whether the biosensor strip 120 has been inserted into the analyte measurement device 110. More specifically, the analyte measurement device 110 may recognize and determine whether the biosensor strip 120 has been inserted into the analyte measurement device 110 by applying a strip recognition signal through the strip recognition electrodes 131 and the recognition reference electrode 133, and a response signal in response thereto is provided from the biosensor strip 120 through the strip recognition electrodes 131 and the recognition reference electrode 133.

[0066] In step 520, when the biosensor strip 120 is inserted into the analyte measurement device 110 (when it is recognized in step 510 that the biosensor strip 120 has been inserted into the analyte measurement device 110), the analyte measurement system 100 (more precisely, the analyte measurement device 110) may use the analyte recognition electrodes 132 included in the biosensor strip 120 to recognize whether the analyte has come into contact with the biosensor strip 120. More specifically, the analyte measurement device 110 may apply an analyte recognition signal through the analyte recognition electrodes 132 and the recognition reference electrodes 133, and a response signal in response thereto may be provided from the biosensor strip 120 through the analyte recognition electrodes 132 and the recognition reference electrodes 133, thereby recognizing and determining whether the analyte has come into contact with the biosensor strip 120.

[0067] In step 530, if the analyte comes into contact with the biosensor strip 120 (if it is determined in step 520 that the analyte has come into contact with the biosensor strip 120), the analyte measurement system 100 (more precisely, the analyte measurement device 110) may apply an analyte measurement signal to the working electrode 134 and working reference electrode 135 included in the biosensor strip 120 and measure a response signal for the analyte, as shown in Figure 6(e). More specifically, the analyte measurement device 110 may apply the analyte measurement signal through the working electrode 134 and working reference electrode 135, and a response signal in response thereto may be provided from the biosensor strip 120 through the working electrode 134 and working reference electrode 135, thereby measuring the response signal in response to the analyte measurement signal, thereby measuring the analyte.

[0068] In particular, step 530 of measuring a response signal to the analyte can be performed independently from step 510 of recognizing whether the biosensor strip 120 has been inserted into the analyte measurement device 110 and step 520 of recognizing whether the analyte has come into contact with the biosensor strip 120, by providing the strip recognition electrode 131 and the analyte recognition electrode 132 separately so that they operate electrically and independently from the working electrode 134 and the working reference electrode 135.

[0069] Although the embodiments have been described above based on limited examples and drawings, those skilled in the art will appreciate that various modifications and variations may be made from the above description. For example, the described techniques may be performed in an order different from that described, and / or the described system, structure, device, circuit, or other element may be coupled or combined in a manner different from that described, or may be substituted or replaced by other elements or equivalents, and still achieve suitable results.

[0070] Therefore, different embodiments are within the scope of the appended claims, provided that they are equivalent to the claims.

Claims

1. 1. An electrode structure of a biosensor strip for measuring an analyte, comprising: It is used to recognize that the biosensor strip has been inserted into a sample measurement device. Strip recognition electrodes, an analyte recognition electrode used to recognize the analyte; and a working electrode and a working reference electrode used to measure the analyte An electrode structure of a biosensor strip, comprising:

2. The strip recognition electrode and the analyte recognition electrode are The working electrode and the working reference electrode are provided separately so as to operate electrically separately. The electrode structure of the biosensor strip according to claim 1 ,

3. The working electrode is formed in a region corresponding to the sample insertion channel of the biosensor strip. The working protrusion and the working reference electrode are formed in a region corresponding to the sample insertion channel. At least two action reference protrusions are In one direction perpendicular to the direction in which the working electrode and the working reference electrode are extended, The biosensor stack according to claim 1, characterized in that the electrodes are arranged alternately in a protruding state. Lip electrode structure.

4. A recognition reference electrode for each of the strip recognition electrode and the analyte recognition electrode is The strip recognition electrode and the sample recognition electrode are provided in common. The electrode structure of the biosensor strip according to claim 1 ,

5. The sample recognition electrode is formed in a region of the biosensor strip corresponding to the sample insertion channel. The sample recognition protrusion and the recognition reference electrode are connected to the sample insertion flow of the biosensor strip. The recognition reference protrusion formed in the area corresponding to the path is The sample recognition electrode and the recognition reference electrode are orthogonal to each other in a direction in which they are extended.

5. The bipolar plate according to claim 4, wherein the bipolar plates are arranged facing each other with their protruding sides facing in the opposite direction. Electrode structure of the sensor strip.

6. a dummy electrode provided on one side of the sample insertion channel of the biosensor strip; The electrode structure of the biosensor strip according to claim 1, further comprising: 。

7. The strip recognition electrode, the analyte recognition electrode, the working electrode, and the working reference electrode 、 2. The method according to claim 1, wherein the plurality of electrodes are formed parallel to each other and spaced apart from each other, extending in one direction. Electrode structure of the biosensor strip shown.

8. 1. A biosensor strip for measuring an analyte, comprising: It is used to recognize that the biosensor strip has been inserted into a sample measurement device. a strip recognition electrode used to recognize an analyte; an analyte recognition electrode used to measure said analyte; a bottom plate having an electrode structure including a working electrode and a working reference electrode used to determine A sample is placed on the upper surface of the lower plate and an enzyme compound that reacts with the sample is inserted. a midplate containing an insertion channel; and When the sample is placed on the middle plate, an insert for inserting the sample into the sample insertion channel is provided. Upper plate including entrance A biosensor strip comprising:

9. The strip recognition electrode and the analyte recognition electrode on the bottom plate are The working electrode and the working reference electrode are provided separately so as to operate electrically separately. The biosensor strip according to claim 8 .

10. Recognition electrodes for the strip recognition electrode and the sample recognition electrode on the lower plate The reference electrode is The strip recognition electrode and the sample recognition electrode are provided in common. The biosensor strip according to claim 8 .

11. The lower plate has: The biosensor strip further includes a dummy electrode provided on one side of the sample insertion channel. The biosensor strip according to claim 8, comprising:

12. It is used to recognize that the biosensor strip has been inserted into a sample measurement device. a strip recognition electrode used to recognize an analyte; an analyte recognition electrode used to measure said analyte; A biosensor having an electrode structure including a working electrode and a working reference electrode used to measure Sustrip, and The biosensor strip is inserted into the sample measuring device. When a biosensor strip is inserted, the sample contacts the biosensor strip. When the sample contacts the biosensor strip, Applying an analyte measurement signal to the electrode and the working reference electrode to measure a response signal to the analyte. The specimen measuring device 1. A analyte measurement system comprising:

13. The method is performed by a sample measurement system including a biosensor strip and a sample measurement device. A sample measurement method comprising: The analyte measurement is performed using a strip recognition electrode included in the biosensor strip. Recognizing whether the biosensor strip is inserted into a device; When the biosensor strip is inserted into the analyte measurement device, the biosensor The biosensor strip is contacted with a sample using a sample recognition electrode included in the strip. The stage of recognizing whether or not something has been touched, and When the sample contacts the biosensor strip, the biosensor strip applying an analyte measurement signal to a working electrode and a working reference electrode included in Measuring the signal A method for measuring a specimen, comprising:

14. The step of measuring a response signal to the analyte comprises: The strip recognition electrode and the analyte recognition electrode are connected to the working electrode and the working reference electrode. The sample measuring device is provided with a separate electrode so as to be electrically and independently operable. A step of recognizing whether the biosensor strip is inserted and the biosensor The step of determining whether a sample has contacted the strip is performed independently of the step of determining whether a sample has contacted the strip. The specimen measurement method according to claim 13 .

15. The dummy electrode provided on one side of the sample insertion channel of the biosensor strip is used to The sample is introduced into the sample insertion channel of the biosensor strip to a size greater than or equal to a predetermined size. Wide-reaching stage The method for measuring a specimen according to claim 13, further comprising:

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