Method for manufacturing demineralized hard tissue and demineralization kit

Abstract

To provide a method for manufacturing a demineralized hard tissue that can reduce labor required for replacing a demineralization solution, and alternatively, to provide a method for manufacturing a demineralized hard tissue that can stabilize the quality of demineralized hard tissues obtained when a plurality of demineralized hard tissues are manufactured.SOLUTION: One embodiment of the present invention includes a method for manufacturing at least two demineralized hard tissues, the method including: an immersion step of immersing at least two hard tissues in a demineralization solution within a single liquid-impermeable container, the at least two hard tissues being immersed in a state in which each of the hard tissues is identifiable.SELECTED DRAWING: Figure 3

Description

[Technical Field]

[0001] The present invention relates to a method for producing demineralized hard tissue and a demineralization kit. [Background technology]

[0002] In the fields of medicine and life science, when preparing hard tissue samples such as pathological specimens, decalcification is usually performed to remove calcium components from the hard tissue. Patent Document 1 is an example of a document that discloses such a decalcification technique. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2005-345194 Summary of the Invention [Problem to be solved by the invention]

[0004] In decalcification, hard tissues are typically immersed in a decalcification solution. Because calcium components in the hard tissue dissolve into the decalcification solution over time, efficient decalcification requires changing the decalcification solution several to several dozen times during the process. This number of solution changes is significantly greater than the number of solution changes required for other sample preparation procedures (such as degreasing).

[0005] In conventional techniques, for the purpose of identifying individual hard tissues, decalcification was performed by placing one hard tissue in one container (see, for example, Figure 1 of Patent Document 1). With this decalcification method, as the number of samples to be prepared increased, the number of containers requiring replacement of the decalcification solution also increased, making the operation extremely cumbersome. Furthermore, with this decalcification method, each sample was decalcified in a different environment, which could result in variations in sample quality.

[0006] An object of one aspect of the present invention is to provide a method for producing a decalcified hard tissue and a decalcification kit that can reduce the effort required for changing the decalcification solution.An object of another aspect of the present invention is to provide a method for producing a decalcified hard tissue and a decalcification kit that can stabilize the quality of the decalcified hard tissues obtained when producing multiple decalcified hard tissues. [Means for solving the problem]

[0007] The present invention includes the following aspects. <1> 1. A method for producing two or more demineralized hard tissues, comprising: The method includes a step of immersing two or more hard tissues in a decalcification solution in a single liquid-impermeable container, The two or more hard tissues are immersed in a state where each of the hard tissues can be identified. Manufacturing method. <2> The hard tissue is surrounded by a liquid-permeable material. <1> The manufacturing method described in <3> The liquid-permeable material is provided with one or more storage compartments; Each of the storage sections contains at most one of the hard tissues. <2> The manufacturing method described in <4> The hard tissues are associated with identifiers for identifying each of the hard tissues. <1> ~ <3> 1. The manufacturing method according to any one of the preceding claims. <5> Meets (i) and / or (ii) below: <4> The manufacturing method described in (i) each of the hard tissues is labeled with the identifier; (ii) the hard tissue is contained in a container provided in a liquid-permeable material, and the identifier is associated with the container (provided that each container contains at most one of the hard tissues). <6> The liquid-impermeable container is sealable. <1> ~ <5> The manufacturing method according to any one of the above. <7> The liquid permeable material is sealable to prevent unraveling of the hard tissue surrounding it. <2> ~ <5> 1. The manufacturing method according to any one of the preceding claims. <8> The liquid-impermeable container and / or the liquid-permeable material are soft materials; <2> ~ <7> 1. The manufacturing method according to any one of the preceding claims. <9> The method further includes a step of sealing the liquid-permeable material with a sealing portion, At least a part of the sealing portion also functions as an attachment portion for a tag including an identifier for identifying the hard tissue. <2> ~ <8> 1. The manufacturing method according to any one of the preceding claims. <10> The hard tissue has one or more joints, The method further includes a step of sealing the liquid-permeable material with a sealing portion, At least a part of the sealing portion also functions as a restraining portion that limits deformation of the joint portion. <2> ~ <9> 1. The manufacturing method according to any one of the preceding claims. <11> Decalcification kit containing: one or more liquid-permeable materials configured to distinctly surround two or more hard tissues; a liquid-impermeable container for containing the liquid-permeable material and decalcifying liquid; <12> Further comprising an identifier for identifying the two or more hard tissues. <11> The kit according to claim 1. [Effects of the Invention]

[0008] According to one aspect of the present invention, there are provided a method for producing a decalcified hard tissue and a decalcification kit, which can reduce the effort required for changing the decalcification solution. According to another aspect of the present invention, there are provided a method for producing a decalcified hard tissue and a decalcification kit, which can stabilize the quality of the decalcified hard tissues obtained when producing multiple decalcified hard tissues. [Brief explanation of the drawings]

[0009] [Figure 1] 1 illustrates an example of a method for making hard tissue identifiable, in which a liquid-permeable material surrounds a piece of hard tissue and a tag containing an identifier. [Figure 2] 10 is a diagram illustrating a state in which the liquid-permeable material is sealed by a sealing portion. FIG. [Figure 3] 1 is a diagram illustrating an example of a method for producing two or more demineralized hard tissues, in which two or more of the liquid-permeable materials depicted in FIG. 2 are contained in a liquid-impermeable container. [Figure 4] 1A-1C illustrate an example of a method for making hard tissue identifiable, in which a liquid-permeable material is provided with identifier markings. [Figure 5] 1 shows an example of a method for making hard tissue identifiable, in which a tag containing an identifier is directly applied to the hard tissue. [Figure 6] 10A and 10B illustrate another example of a liquid permeable material adapted to surround multiple hard tissues. [Figure 7] 10A and 10B illustrate yet another example of a liquid permeable material adapted to surround multiple hard tissues. [Figure 8] 10 is a view showing another embodiment in which the liquid-permeable material is sealed with a sealing portion, and the deformation of the joint portion is restricted by the sealing portion. [Figure 9] 10A and 10B are schematic diagrams illustrating another example of a mode in which deformation of a joint portion is restricted by a sealing portion. [Figure 10] 10A and 10B are schematic diagrams illustrating still another example of a mode in which deformation of a joint portion is restricted by a sealing portion. [Figure 11] 10 is a schematic diagram showing an example of an embodiment in which a part of a sealing portion functions as an attachment portion for a tag including an identifier. FIG. DETAILED DESCRIPTION OF THE INVENTION

[0010] However, the present invention is not limited to the following embodiments and various modifications may be made within the scope of the claims. Embodiments that combine technical means described in different embodiments are also included in the technical scope of the present invention.

[0011] Unless otherwise specified in this specification, "A to B" representing a numerical range means "greater than or equal to A and less than or equal to B." Unless otherwise specified in this specification, elements with the same reference numerals have the same or similar functions.

[0012] [Embodiment 1] 1 to 3, a first embodiment of the present invention will be described. In the first embodiment, one hard tissue 1 and one tag including an identifier 5 are surrounded by a liquid-permeable material 10. Then, a plurality of such liquid-permeable materials 10 are placed in a liquid-impermeable container 50, thereby decalcifying two or more hard tissues in an identifiable state.

[0013] Fig. 1 is a diagram showing a state in which a hard tissue 1 and an identifier 5 are contained in a bag-shaped liquid-permeable material 10 through an opening P. In this way, the hard tissue 1 and the identifier 5 are surrounded by the liquid-permeable material 10. As shown in Fig. 1, each liquid-permeable material 10 in embodiment 1 has one containing section.

[0014] The hard tissue 1 is a hard tissue whose main component is a calcium-containing compound. Examples of hard tissue 1 include bone, cartilage, and teeth. In this specification, hard tissues that have come to contain calcium-containing compounds as their main component due to physiological or pathological causes, such as hard tissues containing calculi and calcified lesions, are also included in the hard tissues.

[0015] The identifier 5 is a code for distinguishing multiple hard tissues 1 from one another. In embodiment 1, a tag including the identifier 5 is surrounded by a liquid-permeable material 10. In order to identify the hard tissue 1, one hard tissue 1 and one tag including the identifier 5 are surrounded by the liquid-permeable material 10. With this configuration, one identifier 5 is associated with one hard tissue 1. The identifier 5 may be a readable code (letters, numbers, symbols, etc.) or an unreadable code (barcode, two-dimensional code, etc.).

[0016] The liquid-permeable material 10 is a material through which a liquid can flow. In one embodiment, the liquid-permeable material is a container having one or more storage compartments. The liquid-permeable material 10 may be made of a liquid-permeable material such as a mesh material. Alternatively, the liquid-permeable material 10 may be made of a liquid-impermeable material such as a plastic film, and may have openings through which a liquid can flow.

[0017] In one embodiment, the liquid-permeable material 10 is a soft material. A soft material refers to a material that is easily deformed when an external force is applied. Examples of soft materials include bags made of mesh material and bags made of plastic film. By using a soft material for the liquid-permeable material 10, space saving is promoted. For example, the liquid-permeable material 10 can be folded for storage. Alternatively, when the liquid-permeable material 10 is immersed in the decalcifying solution 70, excess space is reduced by water pressure.

[0018] In one embodiment, as shown in Fig. 2, the liquid-permeable material 10 may be sealed by providing a sealing portion 15 to prevent the enclosing of the hard tissue 1 from coming undone. That is, the liquid-permeable material 10 may be sealable. The sealing portion 15 may be provided using a sealing member such as a stapler or adhesive. Alternatively, the sealing portion 15 may be provided by fusing a portion of the liquid-permeable material 10.

[0019] By sealing the liquid-permeable material 10, it is possible to prevent the hard tissue 1 from being unwrapped by the liquid-permeable material 10 during the decalcification process (for example, it is possible to prevent the hard tissue 1 from escaping through the opening P of the storage portion). This makes it easy to replace the decalcification liquid 70. To allow the hard tissue 1 to come into contact with the decalcification liquid 70, the sealing portion 15 should not impair the liquid permeability of the liquid-permeable material 10. Furthermore, to make it easy to remove the hard tissue 1 after decalcification, it is preferable that the sealing portion 15 be easy to open or remove.

[0020] In one embodiment, a large opening may be provided in the liquid-permeable material 10. For example, a corner of the liquid-permeable material 10 may be notched along the dashed line C in FIG. 2. Alternatively, a notch may be provided in the liquid-permeable material 10. Alternatively, the liquid-permeable material 10 may be pre-formed into a shape having a large opening. The size of the large opening may be, for example, 1 mm. 2 More than 5mm 2 or more or 10mm 2 or more (when the liquid-permeable material 10 is a soft material, the maximum size that the large opening can have may be within the above-mentioned numerical range). Such a large opening functions as an opening for venting air. Therefore, providing such a large opening allows the hard tissue 1 and the decalcification solution 70 to come into contact with each other efficiently. In particular, when the liquid-permeable material 10 is a fine mesh bag, providing such a large opening may be effective.

[0021] 3 is a diagram showing two or more liquid-permeable materials 10 contained in a liquid-impermeable container 50. As shown in FIGS. 1 and 2, the hard tissue 1 and a tag including an identifier 5 are enclosed within the liquid-permeable material 10. A decalcifying liquid 70 is also poured into the liquid-impermeable container 50. As a result, the hard tissue 1 is immersed in the decalcifying liquid 70 within the liquid-impermeable container 50.

[0022] In the example shown in Figure 3, three hard tissues 1 are decalcified in one liquid-impermeable container 50. Each of the three hard tissues 1 is surrounded by a different liquid-permeable material 10. The liquid-permeable materials 10 also surround tags containing different identifiers 5. This allows the operator to identify the hard tissue 1 being decalcified.

[0023] When replacing the decalcifying liquid 70, for example, the liquid-permeable material 10 is removed from the liquid-impermeable container 50, the decalcifying liquid 70 is replaced, and the liquid-permeable material 10 is re-stored. Alternatively, the liquid-permeable material 10 may be removed from the liquid-impermeable container 50, placed in another liquid-impermeable container 50, and the decalcifying liquid 70 may be poured into the other container. As described above, the manufacturing method according to one embodiment of the present invention can reduce the labor required for replacing the decalcifying liquid (in the prior art, a similar operation required the replacement of the decalcifying liquid to be repeated for each sample). Furthermore, this manufacturing method allows two or more hard tissues 1 to be decalcified in the same environment, making it easier to maintain consistent quality of the resulting samples.

[0024] The decalcified hard tissue produced in this manner can be used for various purposes. According to this production method, the decalcified hard tissue is obtained in a state where two or more hard tissues 1 are distinguished from each other. Therefore, it is preferable to use it in applications where it is important to know which hard tissue 1 each decalcified hard tissue originates from, such as the preparation of pathological specimens. Of course, it may also be used in applications where it is relatively unimportant to know which hard tissue 1 each decalcified hard tissue originates from, such as the extraction of useful components.

[0025] The liquid-impermeable container 50 is a container through which liquid cannot pass from the inside to the outside of the container. Typically, the liquid-impermeable container 50 is made of a liquid-impermeable material such as a plastic film.

[0026] In one embodiment, the liquid-impermeable container 50 is made of a flexible material. A flexible material refers to a container that is easily deformed when an external force is applied. An example of a flexible material is a bag made of a plastic film. Using a flexible material for the liquid-impermeable container 50 helps to save space. For example, the liquid-impermeable container 50 can be folded for storage.

[0027] In one embodiment, the liquid-impermeable container 50 may have a sealing portion 55. That is, the liquid-impermeable container 50 may be sealable. The sealing portion 55 is a member that can seal the liquid-impermeable container 50. Examples of the sealing portion 55 include a sealing zipper, a button, and a string fastener. Providing the liquid-impermeable container 50 with the sealing portion 55 prevents leakage and evaporation of the decalcification solution 70. In embodiment 1, two or more hard tissues 1 are decalcified in one liquid-impermeable container 50, which also reduces the labor required for the sealing and unsealing process (in the prior art, to perform a similar operation, it was necessary to open the container, replace the decalcification solution, and reseal the container, and repeat this process for each sample).

[0028] The decalcifying solution 70 is a liquid for eluting calcium components from the hard tissue 1. Examples of the decalcifying solution 70 include acidic decalcifying solutions and neutral decalcifying solutions. Examples of acidic decalcifying solutions include hydrochloric acid, nitric acid, trichloroacetic acid, formic acid, Planck-Lycros solution, and Morse's solution. Examples of neutral decalcifying solutions include chelating agents such as ethylenediaminetetraacetic acid (EDTA). The type of decalcifying solution 70 and decalcifying conditions can be appropriately determined by those skilled in the art depending on the type and application of the hard tissue 1.

[0029] [Variation 1] FIG. 4 shows a modified example of a method for making two or more hard tissues distinguishable from one another. In FIGS. 1 to 3, two or more hard tissues are made distinguishable from one another by surrounding a tag containing an identifier 5 with a liquid-permeable material 10. Instead, in the modified example shown in FIG. 4, a display portion for the identifier 5 is provided on the liquid-permeable material 10. For example, a tag containing the identifier 5 can be attached to the outer or inner surface of the liquid-permeable material 10 to provide a display portion for the identifier 5. Alternatively, the identifier 5 may be printed in advance on a portion of the liquid-permeable material 10 to serve as a display portion. Alternatively, a writable area may be provided on a portion of the liquid-permeable material 10. In this case, an operator writes the identifier 5 in the writable area, and that area functions as a display portion for the identifier 5.

[0030] [Variation 2] FIG. 5 shows a modified example of a method for making two or more hard tissues 1 distinguishable from one another. In the modified example shown in FIG. 5, a tag containing an identifier 5 is attached directly to the hard tissue 1. For example, two or more hard tissues 1 may be made distinguishable from one another by attaching a label with the identifier 5 printed thereon (or a label with the identifier 5 written thereon) to the hard tissue 1. In the case of modified example 2, since the identifier 5 is attached directly to the hard tissue 1, the liquid-permeable material 10 is not an essential element. In other words, the hard tissue 1 may be directly contained in the liquid-impermeable container 50. From the viewpoint of facilitating the exchange of the decalcification solution 70, it is preferable to surround two or more hard tissues 1 with one liquid-permeable material 10 and to contain one or more of these liquid-permeable materials 10 in the liquid-impermeable container 50.

[0031] [Embodiment 2] FIG. 6 is a diagram illustrating an example of a liquid-permeable material 10a according to a second embodiment. The liquid-permeable material 10a according to the second embodiment is a bag-shaped container having two or more storage sections per container. Specifically, in the example shown in FIG. 6, the liquid-permeable material 10a has a pocket P1, a pocket P2, and a pocket P3, for a total of three storage sections. Each storage section can store up to one hard tissue 1. Therefore, the liquid-permeable material 10a can store up to three hard tissues 1 (some storage sections may not store a hard tissue 1). However, when an identifier 5 is directly associated with the hard tissue 1 as in the second modification, two or more hard tissues 1 may be stored in one storage section, and a total of four or more hard tissues 1 may be stored.

[0032] In the example shown in FIG. 6, as shown in Modification 1, a display unit for the identifier 5 is provided, thereby making two or more hard tissues identifiable from one another. However, other methods may be employed. For example, as shown in Embodiment 1, a tag including the identifier 5 may be surrounded by pocket P1, pocket P2, and / or pocket P3. Alternatively, as in Modification 2, the identifier 5 may be directly associated with the hard tissue 1. Furthermore, since the pockets P1 to P3 are each located at different positions, the two or more hard tissues 1 may be distinguishable from one another by the positions of the pockets P1 to P3, without using the identifier 5.

[0033] [Embodiment 3] FIG. 7 is a diagram illustrating an example of a liquid-permeable material 10b according to a third embodiment. The liquid-permeable material 10b according to the third embodiment is a container having bag-shaped storage sections, with each container having two or more storage sections. Specifically, in the example illustrated in FIG. 7, the liquid-permeable material 10b has pockets P1 to P6 arranged on a plane, with a total of six storage sections. Each storage section can accommodate a maximum of one hard tissue 1 (some storage sections may not accommodate a hard tissue 1). Therefore, the liquid-permeable material 10b can accommodate a maximum of six hard tissues 1. However, when an identifier 5 is directly associated with the hard tissue 1 as in the second modification, two or more hard tissues 1 may be accommodated in one storage section, and a total of seven or more hard tissues 1 may be accommodated.

[0034] In the example shown in FIG. 7, as shown in Modification 1, a display unit for the identifier 5 is provided, thereby making two or more hard tissues identifiable from one another. However, other methods may be employed. For example, as shown in Embodiment 1, a tag including the identifier 5 may be surrounded by one or more of the pockets P1 to P6. Alternatively, as in Modification 2, the identifier 5 may be directly associated with the hard tissue 1. Furthermore, since the pockets P1 to P6 are located in different positions, the two or more hard tissues 1 may be distinguishable from one another by the positions of the pockets P1 to P6, without using the identifier 5.

[0035] [Embodiment 4] FIG. 8 shows another aspect of sealing the liquid-permeable material 10 with the sealing portions 15. In the fourth embodiment, the hard tissue 1 having the joint A is decalcified. At this time, the deformation of the joint A is restricted by the sealing portions 15. In the example of FIG. 8, the deformation (flexion, extension, etc.) of the joint A is restricted by providing the sealing portions 15 in four locations. Therefore, at least some of the sealing portions 15 also function as restraining portions that restrict the deformation of the joint A. The number of sealing portions 15 is not particularly limited as long as it is sufficient to restrict the deformation of the joint A.

[0036] Joint A is a site where two or more bones are connected via tendons, ligaments, cartilage, etc. The two or more bones can change their relative positions at joint A. Therefore, joint A in this specification includes hemijoints in addition to movable joints, but does not include immovable joints. Joint A can be a uniaxial joint (hinge joint, spiral joint, pivot joint, etc.), a biaxial joint (condylar joint, ellipsoid joint, saddle joint, plane joint, etc.), or a multiaxial joint (ball-and-socket joint, socket joint, etc.). In one embodiment, joint A is a joint that can flex and extend.

[0037] When joint A has multiple movement modes (for example, when joint A can perform flexion-extension movement and abduction-adduction movement), sealing portion 15 only needs to restrict at least one of the movement modes. In one embodiment, sealing portion 15 restricts deformation of joint A associated with flexion-extension movement.

[0038] The sealing portion 15 does not need to completely restrict deformation of the joint portion A. In one embodiment, the deformation of the joint portion A is restricted to no more than 70%, no more than 50%, no more than 30%, or no more than 10% of the maximum range of motion.

[0039] Decalcifying the hard tissue 1 in this state reduces changes in the positional relationship of the bones via the joint A before and after decalcification of the hard tissue 1. Therefore, the hard tissue 1 can be decalcified in a shape that is convenient for subsequent processes (such as an embedding process and a thin-sectioning process for preparing a thin section specimen).

[0040] [Variation 3] The sealing portion 15, which also functions as a restraining portion, may seal the opening P of the bag-shaped liquid-permeable material 10. In the modification shown in FIG. 9, after the hard tissue 1 is placed into the liquid-permeable material 10c (a small bag-shaped container) through the opening P, the sealing portion 15 is provided to seal the opening P and limit deformation of the joint portion A. Here, of the five restraining portions, at least two near the opening P of the bag also function as sealing portions 15 to prevent the opening P of the bag from opening and unravelling the enclosure of the hard tissue 1.

[0041] [Variation 4] In the example shown in FIG. 10, a sheet-like liquid-permeable material 10d is used instead of a bag-like material. Specifically, one sheet of the liquid-permeable material 10d is folded and the hard tissue 1 is sandwiched between the folded sheets to surround the material (upper panel). Then, a sealing portion 15 is provided to maintain the two overlapping layers of the liquid-permeable material 10b (lower panel). The sealing portion 15 is provided at a position that can limit deformation of the joint A and prevent the hard tissue 1 from falling off. In the example shown in FIG. 5, the sealing portion 15 also functions as a stopper that limits deformation of the joint A.

[0042] [Embodiment 5] The sealing portion 15 may also serve as an attachment portion for a tag including an identifier 5 for identifying the hard tissue 1. With this configuration, the tag including the identifier 5 can be further prevented from being unwrapped from the liquid-permeable material 10.

[0043] 6, one of the sealing portions 15 prevents the liquid-permeable material 10 from unraveling, and also serves as an attachment portion for a tag including an identifier 5. When the sealing portion 15 is formed by arranging a restraining member (such as a stapler or adhesive), the tag including the identifier 5 is made of a material and has a shape that allows the sealing member to be used. When the sealing portion 15 is formed by fusing the liquid-permeable material 10, the tag including the identifier 5 is made of a material and has a shape that allows it to be fused together with the liquid-permeable material 10.

[0044] [Embodiment 6] Embodiments of the present invention also include a demineralization kit. The demineralization kit includes one or more liquid-permeable materials 10 and a liquid-impermeable container 50. The liquid-permeable material 10 is configured to surround two or more hard tissues 1 in a distinguishable manner. The liquid-impermeable container 50 is for containing the liquid-permeable material 10 and a demineralization liquid 70. In addition to (or instead of) the liquid-permeable material 10, the demineralization kit may include a liquid-permeable material 10a, a liquid-permeable material 10b, a liquid-permeable material 10c, and / or a liquid-permeable material 10d.

[0045] It is also preferable to provide a plurality of storage portions such as liquid-permeable materials 10a and 10b in order to identifiably surround two or more hard tissues 1. In addition to (or instead of) this, it is also preferable to associate the identifier 5 with the hard tissue 1 in order to identifiably surround two or more hard tissues 1 (see the above-described embodiment and modified examples for specific association methods).

[0046] In addition to the liquid-permeable material 10 and the liquid-impermeable container 50, the decalcification kit may further include one or more of an identifier 5, a decalcification solution 70, and other elements useful in the kit. In one embodiment, the kit includes a tag or label that includes the identifier 5, or a tag or label on which the identifier 5 can be written. Examples of elements useful in the kit include instructions describing how to use the kit (e.g., how to decalcify hard tissue 1), and information (e.g., a URL) for obtaining the instructions. The elements included in the decalcification kit may have one or more of any of the features described in embodiments 1 to 3. [Explanation of symbols]

[0047] 1:Hard tissue 5: Identifier 10:Liquid permeable material 10a: Liquid permeable material 10b: Liquid permeable material 10c: Liquid permeable material 10d: Liquid permeable material 15: Sealing part 50 :Liquid impermeable container 55: Sealed part 70: Decalcification solution

Claims

1. 1. A method for producing two or more demineralized hard tissues, comprising: The method includes a step of immersing two or more hard tissues in a decalcification solution in a single liquid-impermeable container, The two or more hard tissues are immersed in a state where each of the hard tissues can be distinguished. Manufacturing method.

2. The hard tissue is surrounded by a liquid-permeable material. The method of claim 1.

3. The liquid-permeable material is provided with one or more reservoirs; Each of the storage sections contains at most one of the hard tissues. The method of claim 2.

4. The hard tissues are associated with identifiers for identifying each of the hard tissues. The method of claim 1.

5. The method of claim 4, which satisfies the following (i) and / or (ii): (i) each of the hard tissues is provided with the identifier; (ii) the hard tissue is contained in a container provided in a liquid-permeable material, and the identifier is associated with the container (provided that each container contains at most one of the hard tissues).

6. The liquid-impermeable container is sealable. The method according to any one of claims 1 to 5.

7. The liquid permeable material is sealable to prevent unraveling of the hard tissue surrounding it. The method of claim 2, 3 or 5.

8. The liquid-impermeable container and / or the liquid-permeable material are soft materials; The method of claim 2, 3 or 5.

9. The method further includes a step of sealing the liquid-permeable material with a sealing portion, At least a part of the sealing portion also functions as an attachment portion for a tag including an identifier for identifying the hard tissue. The method of claim 2.

10. The hard tissue has one or more joints, The method further includes a step of sealing the liquid-permeable material with a sealing portion, At least a part of the sealing portion also functions as a restraining portion that limits deformation of the joint portion. The method of claim 2.

11. A decalcification kit comprising: one or more liquid-permeable materials configured to distinctly surround two or more hard tissues; a liquid-impermeable container for containing the liquid-permeable material and decalcifying liquid;

12. Further comprising an identifier for identifying the two or more hard tissues. The kit of claim 11.

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