Method for improved extraction of spider silk protein

JP2025163077APending Publication Date: 2025-10-28BOLT THREADS INC
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Patent Information

Application Number
JP2025124406
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2019-08-22
Filing Date
2025-07-25
Publication Date
2025-10-28

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Abstract

To provide an improved method for purification of recombinant spider silk protein, thereby enhancing solubility and increasing silk protein recovery yield.SOLUTION: Provided is a method for improving solubilization, extraction, and isolation of a recombinant spider silk protein using a salt and alcohol buffer. Provided is a method for solubilizing a recombinant spider silk protein from a host cell, comprising: providing a cell culture comprising a host cell, the host cell expressing a recombinant spider silk protein; recovering an insoluble portion derived from the cell culture, the insoluble portion comprising the recombinant spider silk protein; and adding the insoluble portion of the host cell to a solution comprising a salt and an alcohol, thereby solubilizing the recombinant spider silk protein in the solution.SELECTED DRAWING: Figure 1
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Citation Information

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