Cosmetic composition

Abstract

To provide a cosmetic composition having high cosmetic effect.SOLUTION: A cosmetic composition comprises pine bark extract, and at least one amino acid selected from proline, arginine, valine, asparagine, aspartic acid, cysteine, isoleucine, methionine, phenylalanine, tryptophan, tyrosine, glycine and alanine.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition containing pine bark extract and other specific ingredients as active ingredients. [Background technology]

[0002] In recent years, research into anti-aging methods to prevent skin aging such as wrinkles has been actively conducted, and collagen and hyaluronic acid have attracted attention. Collagen and hyaluronic acid are known to be produced by fibroblasts that make up the dermis of the skin.

[0003] Compositions that focus on the production of such collagen and hyaluronic acid include, for example, a collagen production promoter characterized by having β-glucan containing β1,3D-glucan (see Patent Document 1), a cosmetic composition for promoting collagen production that contains a cardamom extract (see Patent Document 2), and a composition for promoting hyaluronic acid production that contains an extract extracted from a plant of the family Amaranthaceae (see Patent Document 3). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2015-71588 [Patent Document 2] Japanese Patent Application Laid-Open No. 2015-48338 [Patent Document 3] Japanese Patent Application Laid-Open No. 2013-155140 Summary of the Invention [Problem to be solved by the invention]

[0005] The object of the present invention is to provide a cosmetic composition that can protect the skin by protecting fibroblasts that contribute to the production of collagen and hyaluronic acid, thereby reducing damage to the skin caused by ultraviolet rays and the like. [Means for solving the problem]

[0006] The present inventors have conducted extensive research and studies into the fibroblast-protecting ability of pine bark extract and have found that a high fibroblast-protecting effect can be achieved by combining pine bark extract with specific other ingredients, leading to the completion of the present invention. Specifically, they have found that the fibroblast-protecting ability can be synergistically improved by combining pine bark extract with specific other ingredients that have little or no fibroblast-protecting ability.

[0007] That is, the present invention relates to a cosmetic composition characterized by containing, as active ingredients, a pine bark extract and at least one component selected from the group consisting of the following (a) to (e): (a) at least one functional additive selected from aspartame, maltose, sucrose fatty acid ester, trehalose, reduced palatinose, reduced maltose, and calcium stearate; (b) at least one plant material selected from barley, sweet potato, chou-mei-sou, mugwort, kudzu, black ginger, and rice; (c) at least one amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine; (d) at least one vitamin or mineral selected from vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, sodium pantothenate, niacin, folic acid, biotin, calcium, manganese, zinc, copper, and chromium; (e) at least one cosmetic ingredient selected from hyaluronic acid, placenta, and collagen;

[0008] The cosmetic composition of the present invention has a skin protecting effect and can be used as a skin protecting agent for skin protection.

[0009] The cosmetic composition of the present invention may be obtained by adding an active ingredient comprising a pine bark extract and at least one component selected from the group consisting of (a) to (e).

[0010] The cosmetic composition of the present invention is preferably for oral administration, and is preferably in the form of a tablet, capsule, powder, granule, or liquid.

[0011] The present invention also relates to a cosmetic method (excluding medical procedures) characterized by ingesting the cosmetic composition of the present invention. [Effects of the Invention]

[0012] The cosmetic composition of the present invention can protect fibroblasts, which contribute to the production of collagen and hyaluronic acid, and protect the skin from damage caused by ultraviolet rays and the like, thereby providing cosmetic effects such as anti-wrinkle and anti-aging. [Brief explanation of the drawings]

[0013] [Figure 1]

[0033] Figure 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + plant material) was applied to normal human fibroblasts (NHDF). The upper left graph shows the results when barley was used as the plant material, the upper right graph shows the results when sweet potato was used as the plant material, the lower left graph shows the results when chou-mei-sou was used as the plant material, and the lower right graph shows the results when mugwort was used as the plant material. [Figure 2] This figure shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + plant material) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results when kudzu was used as the plant material, the graph on the top right shows the results when black ginger was used as the plant material, and the graph on the bottom left shows the results when rice (rice bran) was used as the plant material. [Figure 3]

[0023] Figure 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + functional additives) was applied to normal human fibroblasts (NHDF). The upper left graph shows the results when aspartame was used as the functional additive, the upper right graph shows the results when maltose was used as the functional additive, the lower left graph shows the results when sucrose fatty acid ester was used as the functional additive, and the lower right graph shows the results when trehalose was used as the functional additive. [Figure 4]

[0033] Figure 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + functional additives) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results when reduced palatinose was used as the functional additive, the graph on the top right shows the results when reduced maltose was used as the functional additive, and the graph on the bottom left shows the results when calcium stearate was used as the functional additive. [Figure 5]

[0033] Figure 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + amino acids) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results when alanine was used as the amino acid, the graph on the top right shows the results when arginine was used as the amino acid, the graph on the bottom left shows the results when asparagine was used as the amino acid, and the graph on the bottom right shows the results when aspartic acid was used as the amino acid. [Figure 6] 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + amino acids) was applied to normal human fibroblasts (NHDF). The graph on the upper left shows the results when cysteine ​​was used as the amino acid, the graph on the upper right shows the results when glycine was used as the amino acid, the graph on the lower left shows the results when isoleucine was used as the amino acid, and the graph on the lower right shows the results when methionine was used as the amino acid. [Figure 7]

[0023] Figure 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + amino acids) was applied to normal human fibroblasts (NHDF). The graph on the upper left shows the results when phenylalanine was used as the amino acid, the graph on the upper right shows the results when proline was used as the amino acid, the graph on the lower left shows the results when tryptophan was used as the amino acid, and the graph on the lower right shows the results when tyrosine was used as the amino acid. [Figure 8] FIG. 1 shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + amino acid) was applied to normal human fibroblasts (NHDF), and valine was used as the amino acid. [Figure 9] This is a graph showing the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamins and minerals) was applied to normal human fibroblasts (NHDF). The upper left graph is for vitamin A, the upper right graph is for vitamin B1, the lower left graph is for vitamin B2, and the lower right graph is for vitamin B6. [Figure 10] This figure shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamins and minerals) was applied to normal human fibroblasts (NHDF). The upper left graph is for vitamin B12, the upper right graph is for vitamin C, the lower left graph is for vitamin D3, and the lower right graph is for vitamin E. [Figure 11]This is a graph showing the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamins and minerals) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results when sodium pantothenate was used as the vitamin, the graph on the top right shows the results when niacin was used as the vitamin, the graph on the bottom left shows the results when folic acid was used as the vitamin, and the graph on the bottom right shows the results when biotin was used as the vitamin. [Figure 12] This is a graph showing the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamins and minerals) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results when calcium was used as the mineral, the graph on the top right shows the results when manganese was used as the mineral, the graph on the bottom left shows the results when zinc was used as the mineral, and the graph on the bottom right shows the results when copper was used as the mineral. [Figure 13] This figure shows the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + vitamins and minerals) was applied to normal human fibroblasts (NHDF), and chromium was used as the mineral. [Figure 14] This is a graph showing the results of the inhibition rate of cell damage caused by oxidative stress when the cosmetic composition of the present invention (pine bark extract + cosmetic ingredients) was applied to normal human fibroblasts (NHDF). The graph on the top left shows the results using hyaluronic acid as the cosmetic ingredient, the graph on the top right shows the results using placenta as the cosmetic ingredient, and the graph on the bottom left shows the results using collagen as the cosmetic ingredient. DETAILED DESCRIPTION OF THE INVENTION

[0014] The beauty composition of the present invention is characterized by containing a pine bark extract and at least one component selected from the group consisting of the following (a) to (e) (hereinafter sometimes referred to as other components) as active ingredients. Due to the synergistic effect of such a combination of active ingredients, the beauty composition of the present invention can more effectively protect fibroblasts from oxidative stress, suppress the decrease in the production of collagen and hyaluronic acid, reduce skin damage caused by ultraviolet rays, etc., and effectively prevent or improve symptoms such as wrinkles and aging.

[0015] The beauty composition of the present invention has a protective effect on the skin (dermis) and can be a skin protectant used for skin protection. Further, the beauty composition of the present invention can also be used as a collagen production promoter or a hyaluronic acid production promoter.

[0016] [Pine bark extract] The pine bark extract, which is an active ingredient of the beauty composition of the present invention, contains proanthocyanidins as one of the main components. Proanthocyanidins are a group of compounds composed of polycondensates with a degree of polymerization of 2 or more having flavan-3-ol and / or flavan-3,4-diol as constituent units.

[0017] Examples of the raw materials for the pine bark extract used in the present invention include Pinus maritima, Larix kaempferi, Larix gmelinii, Pinus densiflora, Pinus parviflora, Pinus thunbergii, Pinus koraiensis, Pinus taiwanensis, Pinus luchuensis, Pinus sylvestris var. mongolica, Pinus bungeana, etc. Among these, Pinus maritima is preferred. The obtained extract may be concentrated or dried as needed to be in a liquid, paste, or powder form. Also, commercially available products of pine bark extract can be used, for example, the pine bark extract manufactured by Toyo Pharmaceutical Co., Ltd. can be used.

[0018] Furthermore, the pine bark extract used in the present invention preferably contains, as proanthocyanidins, condensation polymers having a degree of polymerization of 2 or more. In particular, proanthocyanidins containing a large amount of condensation polymers having a low degree of polymerization are preferred. Examples of condensation polymers with a low degree of polymerization include condensation polymers (dimers to tridecamers) having a degree of polymerization of 2 to 30, preferably condensation polymers (dimers to decamers) having a degree of polymerization of 2 to 10, and more preferably condensation polymers (dimers to tetramers) having a degree of polymerization of 2 to 4. In this specification, polymers having a degree of polymerization of 2 to 4 are referred to as OPCs (oligomeric proanthocyanidins). The pine bark extract used in the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and even more preferably 40% by mass or more.

[0019] Pine bark extract is obtained by extracting pine bark with water or an organic solvent. When water is used, warm or hot water is preferably used. Organic solvents used for extraction include methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, aqueous ethanol, and aqueous propylene glycol, which are organic solvents acceptable for the production of foods or pharmaceuticals. These water and organic solvents may be used alone or in combination of two or more solvents. Water, ethanol, aqueous ethanol, and the like are particularly preferred.

[0020] The method for extracting a pine bark extract from pine bark is not particularly limited, and may be, for example, a heat extraction method, a supercritical fluid extraction method, etc. A combination of extraction methods may be used to extract a pine bark extract.

[0021] [Other ingredients] (a) Functional additives In the cosmetic composition of the present invention, it is preferable to use, together with the pine bark extract, at least one functional additive selected from aspartame, maltose, sucrose fatty acid ester, trehalose, reduced palatinose, reduced maltose, and calcium stearate as an active ingredient. For example, aspartame, maltose, trehalose, reduced palatinose, and reduced maltose are used as sweeteners, and maltose, trehalose, reduced palatinose, and reduced maltose are also used as excipients. In addition, sucrose fatty acid ester and calcium stearate are used as lubricants, etc.

[0022] (b) Plant material In the cosmetic composition of the present invention, it is preferable to use at least one plant material selected from barley, sweet potato, chou-mei-so, mugwort, kudzu, black ginger, and rice as an active ingredient together with the pine bark extract.

[0023] These plant materials may be any part of the plant, such as leaves, stems, roots, flowers, fruits, trunks, or branches. In addition to the plant material itself (including dried products), processed plant material products such as crushed products, juice, and extracts can be used. Examples of crushed products include powders and granules. Juices and extracts may be in liquid form, but can also be used as pastes or dry powders. Extracts can be obtained by extraction using an appropriate solvent, and examples of solvents that can be used include water (warm water or hot water), ethanol, and aqueous ethanol. These plant materials can be commercially available.

[0024] As barley, two-rowed barley, six-rowed barley, etc. As the plant material of the present invention, stems and leaves are preferred, and young leaves are particularly preferred.

[0025] Sweet potato refers to a plant belonging to the Convolvulaceae family, commonly known as Ipomoea batatas. There are no particular limitations on the variety of sweet potato, and examples include Suio, Joy White, Koganesengan, Shiroyutaka, Satsuma Starch, and Ayamurasaki. The parts of the plant used as the plant material of the present invention are preferably the stems and leaves, and particularly young leaves.

[0026] Chomeisou is a perennial plant also known as Peucedanum japonicum, belonging to the genus Peucedanum of the family Apiaceae. The parts of the plant that can be used as the plant material of the present invention are preferably the leaves and roots.

[0027] Artemisia vulgaris is a perennial plant of the Asteraceae family. The parts of artemisia vulgaris that can be used as the plant material of the present invention are preferably the stems and leaves.

[0028] Kudzu is a climbing perennial plant of the genus Pueraria in the family Fabaceae. The parts used as the plant material of the present invention are preferably flowers or roots, with flowers being particularly preferred. Kudzu flowers may be harvested at any stage, from buds to fully opened flowers, or a mixture of flowers harvested at each stage may be used. The type of kudzu is not particularly limited, but examples include Pueraria thomsonii, Pueraria lobata, and Pueraria thunbergiana.

[0029] Black ginger, whose scientific name is Kaempferia Parviflora, is a plant of the genus Kaempferia in the family Zingiberaceae that grows wild in Southeast Asia. The part of the plant used as the plant material of the present invention is preferably the rhizome.

[0030] Rice is a plant of the genus Oryza in the family Poaceae. The parts used as the plant material of the present invention are preferably the husk and embryo of brown rice, and more specifically, rice bran, which is obtained as a by-product during the polishing of brown rice, is preferably used. The type of rice is not particularly limited, and examples include japonica and indica varieties.

[0031] (c) amino acids In the cosmetic composition of the present invention, it is preferable to use, together with the pine bark extract, at least one amino acid (including a salt) selected from alanine, arginine, asparagine, aspartic acid, cysteine, glycine, isoleucine, methionine, phenylalanine, proline, tryptophan, tyrosine, and valine as an active ingredient. Examples of the salt include sodium salt and hydrochloride.

[0032] (d) Vitamins and minerals In the cosmetic composition of the present invention, it is preferable to use, together with the pine bark extract, at least one vitamin or mineral selected from the group consisting of vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, vitamin E, sodium pantothenate, niacin, folic acid, biotin, calcium, manganese, zinc, copper, and chromium as an active ingredient. Note that calcium, manganese, zinc, copper, and chromium as minerals in the present invention include compounds containing these metals.

[0033] (e) Beauty ingredients In the cosmetic composition of the present invention, it is preferable to use at least one cosmetic ingredient selected from hyaluronic acid, placenta, and collagen as an active ingredient together with the pine bark extract.

[0034] As the hyaluronic acid, for example, the one extracted from animal tissue such as chicken comb, or the one produced by microorganisms such as lactic acid bacteria can be used.Chicken comb has been used as food for a long time, and there is no problem in terms of safety, so chicken comb pulverized material can be used instead of hyaluronic acid.

[0035] The placenta may be an extract of placenta derived from mammals such as humans, pigs, horses, etc. The extraction method may be a conventionally known method, and the form may be liquid, powder, or the like, without particular limitation.

[0036] Collagen may be animal-derived or synthetic, and includes collagen protein, collagen peptide obtained by hydrolyzing collagen protein, and atelocollagen obtained by treating collagen molecules with protease to remove telopeptide portions. Collagen derived from fish is particularly preferred as animal-derived collagen. The average molecular weight (weight-average molecular weight) of collagen is not particularly limited, but is preferably 500 to 100,000, and more preferably 1,000 to 50,000.

[0037] The cosmetic composition of the present invention is not particularly limited as long as it contains pine bark extract and other specified ingredients and can be distinguished from other products in terms of its use in cosmetics. For example, the scope of the present invention includes products that display a cosmetic effect, such as a skin protection effect, on the product itself, packaging, instructions, or promotional materials. Examples of such products include so-called health foods, such as pharmaceuticals (including quasi-drugs), cosmetics, and functional foods whose efficacy has been recognized by a designated organization, such as foods for specified health uses, foods with nutrient functions, and foods with functional claims. Examples of so-called health foods include those that display claims such as "provides a skin barrier," "protects the skin," "for those concerned with blemishes," "for those concerned with wrinkles," "helps skin withstand stress," "helps skin withstand UV rays," "good for beauty," and "good for the skin."

[0038] The cosmetic composition of the present invention can be used orally or externally. There are no particular limitations on the external preparation as long as it is applied to the skin, scalp, etc., and its form may include external skin preparations such as ointments, creams, gels, lotions, emulsions, packs, and poultices, as well as injections.

[0039] Furthermore, when the cosmetic composition of the present invention is used as an oral preparation, its form may be, for example, a tablet, capsule, powder, granule, liquid, granule, bar, plate, block, solid, pill, paste, cream, caplet, gel, chewable, or stick. Among these, tablets, capsules, powder, granule, and liquid forms are particularly preferred. Specific examples include supplements, packaged beverages filled in PET bottles, cans, or bottles, and instant powdered or granular beverages that can be dissolved in water (hot water), milk, fruit juice, green juice, or the like. These are preferred because they are easy to drink during meals and can enhance palatability.

[0040] The pine bark extract and other ingredients (active ingredients) may be contained in the cosmetic composition of the present invention in an amount appropriate to the extent that the desired effect is achieved.

[0041] Generally, when the cosmetic composition of the present invention is a pharmaceutical or supplement (tablet, capsule), the active ingredient is preferably contained in an amount of 0.01 to 100% by mass, more preferably 0.1 to 85% by mass, and even more preferably 0.5 to 70% by mass, of the total mass converted to dry mass.

[0042] When the cosmetic composition of the present invention is a packaged beverage (liquid), the active ingredient is preferably contained in an amount of 0.01 to 15 mass % of the total amount, calculated as dry mass, more preferably 0.03 to 12.5 mass %, and even more preferably 0.05 to 10 mass %.

[0043] Furthermore, when the cosmetic composition of the present invention is an instant powdered drink (powder) or an instant granular drink (granules), the active ingredient is preferably contained in an amount of 0.1 to 80 mass % of the total, calculated on a dry mass basis, more preferably 0.5 to 70 mass %, and even more preferably 1 to 60 mass %.

[0044] To maximize the effects of the present invention, the active ingredient preferably accounts for 80% or more of the total cosmetic composition (excluding water) of the present invention in terms of dry mass, more preferably 90% or more, even more preferably 95% or more, and particularly preferably 100%. Furthermore, when the cosmetic composition of the present invention contains any of components (a) to (c), it is preferable that the components contained therein consist solely of the active ingredient of the present invention. That is, when the cosmetic composition of the present invention contains, for example, component (a) (plant material), it is preferable that it does not contain any plant material other than barley, sweet potato, chomeisou, mugwort, kudzu, black ginger, terminalia, rice, and coconut.

[0045] The intake amount of the cosmetic composition of the present invention is not particularly limited, but from the viewpoint of more significantly exhibiting the effects of the present invention, the daily intake of the active ingredient is preferably 0.1 mg / day or more, more preferably 1 mg / day or more, even more preferably 10 mg / day or more, and particularly preferably 40 mg / day or more. The upper limit is not particularly limited, but is, for example, 4 g / day, preferably 2 g / day.

[0046] The cosmetic composition of the present invention can be stored as a daily dose in one container or divided into, for example, 2 to 3 containers so that the daily intake amount is the above-mentioned amount.

[0047] The blending mass ratio of the pine bark extract to the other ingredients, calculated on a dry mass basis, is preferably in the range of 0.5:1 to 70:1, more preferably in the range of 0.75:1 to 60:1, even more preferably in the range of 1:1 to 60:1, and particularly preferably in the range of 1:1 to 50:1. When the blending ratio of the pine bark extract to the other ingredients is in the above range, the effects of the present invention can be more effectively exhibited.

[0048] The cosmetic composition of the present invention can be produced by known formulation methods, by adding ingredients other than the active ingredients that are acceptable for oral or external use, if necessary.

[0049] Furthermore, examples of the cosmetic composition as an oral preparation of the present invention include not only cosmetic foods containing the active ingredient, but also cosmetic foods obtained by adding the active ingredient to a food, such as foods in which the content of the active ingredient of the present invention is increased compared to ordinary foods, and foods in which the active ingredient of the present invention has been added to foods that do not normally contain the active ingredient of the present invention. The active ingredients may be added separately or simultaneously, or together with other ingredients other than the active ingredient.

[0050] Examples of the beauty foods of the present invention include beverages such as carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, smoothies, and green juice; frozen desserts such as ice cream, ice sherbet, and shaved ice; noodles such as soba, udon, harusame, Chinese noodles, and instant noodles; sweets such as candy, candy, gum, chocolate, tablet sweets, snacks, biscuits, jelly, jam, cream, baked goods, and bread; processed seafood and livestock foods such as kamaboko, ham, and sausage; processed milk, fermented milk, Examples include dairy products such as yogurt; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, and processed foods thereof; seasonings such as sauces and soy sauce; and retort pouch foods such as curry, stew, oyakodon, rice porridge, porridge, Chinese rice bowl, katsudon, tempura bowl, beef bowl, hayashi rice, omelet rice, oden, mapo dolphin, gyoza, shumai, hamburger steak, meatballs, various sauces, and various soups.

[0051] The beauty method of the present invention is characterized by ingestion of the above-described beauty composition of the present invention, but does not include medical procedures. Examples of the method of the present invention include a method in which beauty effects are obtained by providing the beauty food of the present invention in a food service establishment such as a restaurant. Also, examples of the method of the present invention include a method in which the beauty food of the present invention is provided as part of a treatment in an aesthetic salon or the like. [Example]

[0052] The present invention will be described below based on examples. [Example 1] 1.Cell culture (1) In a 37°C, 5% CO2 incubator, 75 cm 2 Normal human fibroblasts (NHDF) were cultured in a flask in 10% FBS-DMEM. (2) Cells suspended by trypsin treatment were collected at 75 cm 2 1.0 x 10 cells were transferred from the flask to each well of a 96-well plate. 4 The cells were seeded at a cell density of 100 cells / well. (3) The cells were pre-cultured for 24 hours in a 37°C, 5% CO2 incubator. (4) After removing the medium from each well, 200 μL of medium containing the test substance adjusted to a predetermined concentration was added to each well, and the cells were cultured in a CO 2 incubator for 24 hours.

[0053] For the samples containing pine bark extract and other ingredients, a 2x concentration of the test substance was prepared and 100 μL of the test substance was added to each sample to achieve the desired concentration. For the control, the test substance dissolved in DMSO was prepared using 10% FBS-DMEM containing 0.25% DMSO, and the other test substances were prepared using 10% FBS-DMEM.

[0054] The pine bark extract used was a hot water extract (dried powder) of the bark of French maritime pine. The other ingredients used together with the pine bark extract were the substances shown in Tables 1 to 5.

[0055] (5) After 24 hours of culture, 50 μL of hydrogen peroxide solution prepared at 2.5 mM in serum-free DMEM was added (final concentration: 500 μM). The control (+) was prepared by adding hydrogen peroxide to the control.

[0056] 2. Measurement of the rate of suppression of cell damage caused by oxidative stress (1) 90 minutes after the addition of hydrogen peroxide, the medium was removed from the cultured cells, and the cells were washed once with 200 μL / well of PBS. 150 μL of Cell Counting Kit-8 solution diluted 30-fold with serum-free DMEM was then added. (2) After allowing the plate to stand in a 37°C, 5% CO2 incubator to allow the plate to develop color appropriately, the absorbance at 450 nm was measured. (3) Based on the obtained data, the cell damage inhibition rate (% of control) relative to the control (+) was calculated.

[0057] % of control = (Data sample-Data blank) / (Data control-Data blank)×100

[0058] The results are shown in Tables 1 to 5 and Figures 1 to 14. From left to right, each figure shows the results for "control (hydrogen peroxide added)," "pine bark extract alone," "other components alone," and "pine bark extract + other components added." The numerical values ​​in the legend below the graphs indicate the sample concentrations; for example, "Pine_10" in the graph at the top left of Figure 1 indicates 10 μg / mL of pine bark extract. The concentration units of the added components are μg / mL in all graphs. The vertical axis indicates "% of control."

[0059] [Table 1]

[0060] For barley, dried and ground powder of young leaves was used. For sweet potatoes, dried and crushed young leaves were used. For Chomeisou, dried and crushed powder of leaves was used. For mugwort, finely ground fresh leaves were used. For kudzu, a hot water extract (dried powder) of the flowers was used. For black ginger, a 60% ethanol extract (dried powder) of black ginger rhizome was used. Commercially available rice bran was used. For the maqui berry, a commercially available dried powder of the fruit was used.

[0061]

Table 2

[0062]

Table 3

[0063]

Table 4

[0064]

Table 5

[0065] As shown in Tables 1 to 5 and Figures 1 to 14, by combining the pine bark extract with specific other components of the present invention, the inhibitory rate of fibroblast damage caused by oxidative stress increased synergistically. Therefore, according to the cosmetic composition of the present invention, fibroblasts can be protected and a high cosmetic effect can be obtained.

[0066] [Example 2] (Production of tablets) Tablets (500 mg) composed of the following components were produced. Reducing maltose 5% by mass Collagen peptide 15% by mass Soybean protein 15% by mass Starch 15% by mass Pine bark extract 8% by mass Vitamin C 1% by mass Vitamin B1 1% by mass Vitamin B2 1% by mass Vitamin B6 1% by mass Folic acid 1% by mass Zinc gluconate 1% by mass Cellulose 2% by mass Calcium chloride 2% by mass Calcium stearate 1% by mass

[0067] [Example 3] (Production of capsules) The following mixture was encapsulated in a soft capsule to produce a capsule formulation. Hyaluronic acid 150mg Maltose 50mg Reduced maltose 50mg Pine bark extract 20mg Silicon dioxide 10mg Vitamin E 0.1mg Niacin 0.5mg Asparagine 0.25mg Aspartic acid 0.25mg Magnesium 1mg

[0068] [Example 4] (Production of Granules) The following ingredients were mixed together and granules were prepared in a conventional manner. 20g fructooligosaccharides Isomaltooligosaccharides 20g Calcium stearate 2g Silicon dioxide 1g Pine bark extract 0.4g Chlorogenic acid 0.1g Niacin 0.01g Zinc gluconate 0.001g

[0069] [Example 5] (Production of instant powder) The following ingredients were mixed and prepared into an instant powder (10 g) in a conventional manner. Trehalose 0.1g Aspartame 0.1g Sucrose fatty acid ester 2g Pine bark extract 0.4g Alanine 0.01g Arginine 0.02g Glycine 0.01g Isoleucine 0.01g Methionine 0.01g Phenylalanine 0.01g Proline 0.01g Tryptophan 0.01g Tyrosine 0.01g Valine 0.02g Glutamine 0.01g Zinc gluconate 0.001g Iron chloride 0.001g Fragrance 0.001g Calcium stearate Remainder

[0070] [Example 6] (Production of liquid preparation) A liquid formulation (50 mL) consisting of the following components was prepared. Pig placenta 2000mg Pine bark extract 400mg Maltose 150mg Asparagine 150mg Aspartic acid 150mg Isoleucine 50mg Proline 50mg Tryptophan 25mg Vitamin C 0.001% by mass Vitamin B1 0.001% by mass Vitamin B6 0.001% by mass Water remaining [Industrial Applicability]

[0071] The cosmetic composition of the present invention has a high cosmetic effect and can be used as an oral agent or an external agent, and therefore the present invention is highly industrially useful.

Claims

[Claim 1] An oral composition comprising, as active ingredients, a pine bark extract and at least one component selected from the group consisting of the following (a) and (b): (a) Barley (b) at least one functional additive selected from reduced maltose, maltose, calcium stearate, sucrose fatty acid ester, aspartame, reduced palatinose, and trehalose; (However, excluding (1) to (5) below) (1) An oral skin moisture-increasing agent containing at least one selected from pine bark extract and reduced coenzyme Q10. (2) A polyphenol-rich aqueous solution containing sugars in an amount equal to or greater than 5 times the amount of polyphenols in terms of anhydrous weight. (3) A powder mixture of polyphenols and sugars in an amount equal to or greater than five times the weight of the polyphenols in anhydrous equivalent. (4) A health food containing processed barley leaves, pine bark extract, brown sugar, and water-soluble dietary fiber. (5) Composition containing flavanoids, vitamin C, vitamin E, and niacin

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