METHOD FOR MEASURING PARTICLES IN SOLUTION AND APPARATUS FOR CARRYING OUT THE SAME

Abstract

The present invention relates to a method for measuring particles present in a solution. The particle measuring device according to the present invention includes a power supply unit for applying a voltage, a chip having a plurality of electrodes arranged in sequence on a microchannel through which a fluid passes, a circuit unit having at least a part of a bridge circuit printed thereon so as to be in a predetermined electrical state with the plurality of electrodes formed on the chip, and a measuring unit in which the power supply unit applies a voltage to the circuit unit and measures a change in an output signal of the bridge circuit due to particles in the fluid passing through the microchannel. According to the present invention, the fine particles in the solution can be accurately measured using the change in the electrical signal. In addition, the properties of the fine particles can be more accurately measured using the magnitude and time of the change in the electrical signal.

Description

[Technical field]

[0001] The present invention relates to a method for measuring particles present in a solution. [Background technology]

[0002] Various particle counting techniques have been developed for the purpose of determining the purity of solutions containing minute particles or for analysis on a cell-by-cell basis.

[0003] For example, in the light scattering method, light is directly transmitted through a sample and then reflected by the surface of particles in the sample, and the number and size of particles are calculated based on the correlation between the amount of light detected and the size or refractive index of the particle to be measured. However, if the surface of the particle is irregular, the degree to which light is reflected may differ depending on the surface, which can cause errors.

[0004] On the other hand, in the method using electrical resistance, a conductive sample is passed through a gap between electrodes, and the electrical pulse generated at this time is used to count the particles.

[0005] Typically, impedance-based flow cytometers use the principle of electrical resistance to measure particle size and distribution. When particles dispersed in an electrolyte solution pass through a small hole in a microchannel, the resistance between two electrodes through which a certain current flows increases, generating a potential difference, which is measured and converted into particle size and distribution.

[0006] However, such flow cytometry requires trained operators in a well-controlled microenvironment, and the complex equipment makes it difficult to use outside of a laboratory environment.

[0007] Therefore, for portable analytical and diagnostic purposes, a more simplified structure needs to be devised. Summary of the Invention [Problem to be solved by the invention]

[0008] The present invention aims to propose a more simplified method for measuring particles in a solution.

[0009] More specifically, the present invention aims to propose a method for measuring particles in a solution by forming a bridge circuit with a chip having a microchannel formed therein and processing the output signals individually.

[0010] More specifically, the present invention aims to propose a method for counting the passage of microscopic particles using an electrical signal sensed via a Wheatstone bridge.

[0011] More specifically, the present invention aims to propose a method for measuring particle characteristics using the magnitude and time of electrical state changes due to input and output signals. [Means for solving the problem]

[0012] The particle measuring device according to the present invention for solving the above technical problems includes a power supply unit that applies a voltage, a chip having a plurality of electrodes arranged in sequence on a micro flow path through which a fluid passes, a circuit unit on which at least a portion of a bridge circuit is printed so as to be in a predetermined electrical state with the plurality of electrodes formed on the chip, and a measuring unit in which the power supply unit applies a voltage to the circuit unit and measures a change in the output signal of the bridge circuit due to particles in the fluid passing through the micro flow path.

[0013] The measurement section detects a phase change in the electrical signal caused by the particle being positioned between the arranged electrodes, and counts the number of particles in the fluid by the number of phase changes.

[0014] It is preferable that the measurement section calculates the size of the particles in the fluid from the magnitude of the amplitude of the phase-shifted electrical signal.

[0015] It is preferable that the device further includes a signal conversion unit that converts the electrical signal applied by the power supply unit into an AC signal, amplifies the converted AC signal, and adjusts it to a predetermined offset, and the measurement unit measures a change in the electrical state by an output signal measured by the bridge circuit after application of the offset-adjusted electrical signal.

[0016] It is preferable that the measurement unit multiplies and amplifies the output signal using the offset-adjusted initial electrical signal, removes a specific frequency signal from the amplified output signal to convert it to DC, and detects an electrical phase value signal due to a change in the electrical state.

[0017] The frequency to be removed is preferably set to a frequency at least similar to the frequency of the original electrical signal.

[0018] It is preferable that the measurement section calculates the transit time of the particle through the flow channel by using a generation period of the DC output signal.

[0019] The particle measurement method according to the present invention for solving the above technical problem includes a step of applying a voltage to a plurality of electrodes arranged in sequence on a microchannel through which a fluid passes and a bridge circuit that is in a predetermined electrical state, and a step of measuring a change in an output signal of the bridge circuit due to particles in the fluid passing through the microchannel.

[0020] The measuring step preferably includes detecting a phase change in the electrical signal caused by the particle being located between the aligned electrodes, and counting the particles in the fluid based on the number of phase changes.

[0021] The measuring step preferably includes calculating a size of a particle in the fluid based on the magnitude of the phase-changed electrical signal.

[0022] The step of applying the voltage includes the steps of converting an electrical signal into an AC signal, amplifying the converted AC signal, adjusting the amplified electrical signal to a predetermined offset, and applying the offset-adjusted electrical signal to the bridge circuit.

[0023] The measuring step includes the steps of calculating the difference between the output signals output from the bridge circuits and amplifying the difference, multiplying and amplifying the amplified difference signal using the offset-adjusted first electrical signal, and removing a specific frequency signal from the amplified output signal to convert it to DC. Effect of the Invention

[0024] According to the present invention, the change in electrical signal can be used to accurately measure the presence of minute particles in a solution.

[0025] Additionally, the magnitude and time of change in the electrical signal can be used to more accurately measure the properties of the microscopic particles.

[0026] Furthermore, the present invention allows for efficient measurement of particle characteristics even at a relatively low voltage using a simple circuit configuration.

[0027] Furthermore, the present invention can be directly applied to clinical diagnosis by quantifying the biological properties of the measured particles using individual reference values.

[0028] Furthermore, the present invention can be widely used as a portable measuring device due to its miniaturization. [Brief description of the drawings]

[0029] [Figure 1] FIG. 1 shows the structure of an apparatus for measuring particle counts according to the present invention; [Diagram 2] FIG. 2 is a diagram showing the structure of a power supply unit of the device for measuring particle values ​​according to the present invention. [Figure 3a] FIG. 4 is a diagram showing a process of a power supply unit for processing an input electrical signal according to the present invention; [Figure 3b] FIG. 4 is a diagram showing a process of a power supply unit for processing an input electrical signal according to the present invention; [Figure 3c] FIG. 4 is a diagram showing a process of a power supply unit for processing an input electrical signal according to the present invention; [Figure 4] FIG. 2 is a diagram showing the structure of a measuring unit of the device for measuring particle values ​​according to the present invention. [Figure 5a] FIG. 4 is a diagram showing the process of measuring an output electrical signal according to the present invention. [Figure 5b] FIG. 4 is a diagram showing the process of measuring an output electrical signal according to the present invention. [Figure 5c] FIG. 4 is a diagram showing the process of measuring an output electrical signal according to the present invention. [Figure 5d] FIG. 4 is a diagram showing the process of measuring an output electrical signal according to the present invention. [Figure 6a] 1 is a diagram showing a process of detecting an electric signal by a particle according to the present invention. [Figure 6b] 1 is a diagram showing a process of detecting an electric signal by a particle according to the present invention. [Figure 6c] 1 is a diagram showing a process of detecting an electric signal by a particle according to the present invention. [Figure 6d] 1 is a diagram showing a process of detecting an electric signal by a particle according to the present invention. [Figure 7] FIG. 2 shows the characteristics of a measured signal for measuring particle counts according to the present invention. [Figure 8] FIG. 2 is a diagram showing the characteristics of an electrode structure for measuring particle counts according to the present invention. [Figure 9] FIG. 2 is a flow diagram of a method for measuring particle counts according to the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0030] The following merely illustrates the principles of the invention. Thus, those skilled in the art can invent various devices that embody the principles of the invention and fall within the concept and scope of the invention, although not explicitly described or illustrated herein. It should also be understood that all conditional terms and embodiments listed herein are expressly intended only for the purpose of making the concept of the invention understandable, and are not limited to the embodiments and conditions specifically listed in this manner.

[0031] The above-mentioned objects, features and advantages will become more apparent from the following detailed description taken in conjunction with the accompanying drawings, which will enable those skilled in the art to which the invention pertains to easily implement the technical ideas of the invention.

[0032] In addition, when describing the invention, if it is determined that a specific description of a known technology related to the invention may make the gist of the invention unclear, the detailed description will be omitted. Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

[0033] FIG. 1 is a diagram showing the structure of an apparatus 100 for measuring particle counts according to the present invention.

[0034] Referring to FIG. 1, a particle measuring device 100 according to the present invention includes a power supply unit 110 that provides power, a circuit unit 120 on which a circuit is printed, and a measuring unit 130 that detects and measures an electrical signal output from the circuit unit 120.

[0035] In addition, the particle measuring device 100 may be configured to include a micro flow path formed between an inlet and an outlet open to the outside on a substrate, and a measuring chip 140 in which a plurality of electrodes are arranged in contact with the flow path.

[0036] The tip 140 can be formed in a structure that can be attached to and detached from the particle measuring device 100, so that a user can measure particles in a solution to be measured by replacing the tip for each measurement.

[0037] Furthermore, the electrodes on the inserted chip 140 form a bridge circuit together with the electrodes of the circuit section, thereby enabling the measuring section 130 to detect even more minute changes in electrical characteristics.

[0038] In this embodiment, the measuring unit 130 measures the electrical signal output from the bridge circuit under a basic state in which the electrolyte solution passes through the micro flow channel but the particles in the solution do not pass between the electrodes. The measuring unit 130 detects the particles from a change in the electrical signal due to a change in electrical characteristics such as impedance caused by the particles in the solution passing between the electrodes on the flow channel.

[0039] Specifically, when particles in a solution are positioned between arranged electrodes, a phase change in an electrical signal is detected, and the number of times the phase-changed signal occurs can be counted.

[0040] Here, in order for the particle measuring device 100 according to this embodiment to be operable using a DC voltage power source from a portable power source such as a dry cell, a pre-processing process can be performed to convert the power applied from the power supply unit to AC before application.

[0041] The pre-processing of the input signal will now be described in more detail with reference to FIG.

[0042] FIG. 2 is a block diagram showing a detailed configuration of a power supply unit according to this embodiment.

[0043] Referring to FIG. 2, in addition to the power supply 112, the power supply section further includes a function generator 114 and a signal conversion section .

[0044] Generally, in the case of a power source for a portable device such as a battery, an electrical signal is output in the form of a DC power source by maintaining a predetermined polarity at the terminals. In this embodiment, the function generator 114 converts the DC signal into the form of an AC signal in order to clearly detect changes in components such as phase caused by particles from the potential difference provided by the power source 112.

[0045] Specifically, the function generator 114 converts the applied voltage into a periodic signal having a continuous waveform with a period of the determined function. The function generator 114 can be realized in various forms, such as a microcontroller unit, a function generating device, etc., as necessary.

[0046] Next, the signal conversion unit 116 amplifies the AC signal having a continuous waveform and adjusts it to a predetermined offset. The amplified AC signal is finally converted by adjusting the offset into a form in which positive and negative voltage values ​​are repeated so as to have a sine wave shape. The AC signal in which positive and negative voltage values ​​are periodically repeated prevents electrolysis of the analysis solution by the power source through the electrodes.

[0047] The signals converted in the above signal processing steps will be described in more detail with reference to FIG.

[0048] 3a, as described above, the voltage output from a battery that is mainly used in portable devices has a certain signal in the form of DC. In the case of a non-periodic DC signal, it is difficult to detect an electrical signal having a slight phase difference according to the characteristics of the material being conducted.

[0049] Therefore, the function generator 114 can change the DC signal of the power supply 112 into a continuous waveform and convert it into a periodic signal of the form shown in FIG. 3b.

[0050] Next, the signal conversion unit 116 amplifies the input signal to increase the amount of change in the electrical signal output from the function generator 114, and at the same time adjusts the offset so that the input signal has a sine wave shape, thereby generating the final input signal as shown in FIG. 3c.

[0051] In other words, the power supply unit 110 according to the present invention solves the problem that the unidirectional electrical signal of batteries commonly used in portable devices causes extreme imbalance of ions in the electrolyte, electrolysis of the solution, and high resistance, by converting the AC signal, and makes it possible to detect the flow of particles in the electrolyte solution using the periodic component of the sinusoidal periodic signal.

[0052] After the periodic signal generated through the above process is applied to the circuit unit 120, the measurement unit 130 detects the change in the magnitude of the voltage, preferably over time, as the electrical signal output from the bridge circuit.

[0053] In this embodiment, the bridge circuit can be designed so that the impedances of the multiple resistors and electrodes are balanced in the Wheatstone bridge, and therefore, more sensitive detection can be achieved by detecting electrical signals generated by state changes in the bridge circuit compared to detection of absolute signal values.

[0054] However, since the electrical signal detected from the bridge circuit is also sensitive to noise, the measuring unit 130 according to this embodiment needs to accurately distinguish the change in the component due to the specific particle to be detected from the change in the signal.

[0055] Therefore, the measuring unit 130 according to the present invention includes a configuration for accurate detection.

[0056] Referring to FIG. 4, the measurement section 130 includes a buffer 132 , an amplifier 134 , a multiplier 136 and a filter 138 .

[0057] Buffer 132 utilizes the characteristic that its input impedance is nearly infinite to ensure that the output signal output from the bridge circuit is not affected by other circuit components of measurement unit 130, and the signal is input to amplifier 134 almost as is.

[0058] The amplifier 134 calculates the difference between the input signals and amplifies the difference value. The input signals may have various noises from the solution, and amplifying the difference value removes the noises temporarily and extracts only the value of the asymmetric signal generated from the particles. The extracted signal is input to the multiplier 136.

[0059] Next, the multiplier 136 multiplies and amplifies the output signal of the amplifier 134. Specifically, the output signals can be amplified by multiplying them together using the first electrical signal having an offset-adjusted sine wave shape. The first signal is used because it has the same signal frequency as the output signal, and if necessary, another signal having the same frequency other than the first signal can be used.

[0060] When a particle in a solution is located between electrodes on a flow path while passing through the flow path, the impedance between the electrodes due to the particle may increase instantaneously, and furthermore, a potential difference may occur due to the particle's role as a capacitor, which may cause a change in the amplitude and phase of the voltage signal.

[0061] Therefore, the multiplier 136 multiplies the initial electrical signal by the output signal to extract a signal having a phase difference, thereby amplifying the waveform signal and at the same time making the phase value a constant. The output of the multiplier 136 is calculated as the sum of an AC wave having a frequency as a variable and a DC wave affected by the phase difference. Therefore, in order to finally extract only the phase difference generated by the particles, the filter 138 filters the AC wave with the output signal of the multiplier 136.

[0062] That is, the process of extracting the pure target signal generated by the particle from the output signal detected by the measuring unit 130 will be described in more detail with reference to FIG.

[0063] Referring to FIG. 5a, the electrical signal output from the first Wheatstone bridge may have a complex waveform that is difficult to interpret due to differences in electrical properties between the solution and the particles.

[0064] Therefore, the buffer 132 compensates for the amount of signal bounce in the output signal to convert it into an interpretable waveform signal. The converted signal has a form as shown in FIG. 5b, and the overall signal appearance can have a form close to a sine wave.

[0065] Here, the buffer 132 uses the characteristic that the input impedance is nearly infinite to prevent loss of the signal value measured while connecting different circuits. The signal value passing through the buffer is amplified by the amplifier 134 to amplify the signal difference value and remove noise primarily. The signal is amplified and extracted using the multiplier 136 to detect electrical component changes due to particles contained in the signal. In this embodiment, to amplify and extract the signal, the input signal and output signal of the power supply unit 110 in the form of an offset-adjusted sine wave can be multiplied as the input signal. The amplified signal in the form of FIG. 5c can be output from the multiplier 136.

[0066] After being compensated through the buffer, the amplified signal passes through the multiplication circuit, where it is mixed with a DC waveform of a constant form corresponding to the frequency component and phase difference. The filter 138 detects a minute phase difference signal generated by the influence of particles by removing the frequency component signal.

[0067] That is, after removing the frequency component signals, the remaining signal has a phase difference as shown in FIG. 5d.

[0068] The output signal, from which the components corresponding to the frequency of the input signal have been removed, is converted to DC in the same form as the signal initially applied by the power source, and therefore the measuring unit 130 can more clearly extract the change components due to particles in the signal.

[0069] As described above, the signal output from the filter 138 has a specific waveform as a particle momentarily appears on the electrode and then disappears, and thus it is possible to determine whether or not a particle has passed through.

[0070] Hereinafter, in this embodiment, a process of generating a specific waveform signal whose phase is changed and detected due to the passage of a particle will be described in more detail with reference to FIG.

[0071] Referring first to Figure 6a, before a particle passes through the electrodes, the voltage signals in the bridge circuit are balanced and in phase with the input electrical signal, so the signal output from filter 138 has a pure DC waveform.

[0072] Next, when the particle advances further and is located between the first electrode and the second electrode, a voltage drop occurs between the electrodes due to the particle acting as a resistor or capacitor, and as a result, the phase θ1 of the voltage V1 decreases compared to the phase θ2 of the voltage V2. Therefore, the electrical signal due to the phase change can be detected as shown in Fig. 6b in a part of the waveform in which the same frequency signal as the input signal is removed via the filter 138. Alternatively, it is also possible to detect the waveform by removing the input signal and similar frequency signals within a predetermined range.

[0073] Next, when the particle passes the first electrode again and is located between the second and third electrodes, a voltage drop occurs between the second and third electrodes, and a difference occurs between the phase θ2 of the voltage V2 and the phase θ1 of the voltage V1. This causes a phase difference again, and the phase of V1 becomes larger than the phase of V2, resulting in a signal being detected in the form of Fig. 6c.

[0074] When the final particle has passed all the electrodes, the state returns to the basic state again and a signal of the form shown in Figure 6d is detected.

[0075] Furthermore, if the particle is a cell, due to the characteristics of the cell, when located between the electrodes, it can act as a capacitor, and therefore the size and characteristics of the particle can affect the capacitance and this characteristic can be reflected in the signal.

[0076] Therefore, in this embodiment, by detecting the waveforms of continuous particles, it is possible to count the number of particles and calculate the size of the particles using the magnitude of the waveform.

[0077] 7, the wavelength of the signal can be calculated from the transit time of the particle, and the signal magnitude can also be calculated from the particle size because the signal magnitude is proportional to the change in voltage caused by the particle acting as a capacitor, as described above.

[0078] In addition, since the above signals are generated from the time when the particle starts to pass through the electrode to the time when the particle completes its passage, it is possible to convert the signals into the time characteristics or the particle passing speed, and calculate further particle characteristics.

[0079] For example, if particles in a solution have elasticity like cells, they can change shape and size according to their original stiffness, and can pass through passages with a width smaller than their original size by reducing the diameter of the body. However, when cells in the human body bind to various substances or become physically stiff due to aging, their elasticity decreases and they become harder.

[0080] Cells with increased rigidity take longer to pass through a passage of the same width, and the particle measuring device of this embodiment can use the proportional relationship between the passage time (wavelength) and factors that affect rigidity to grasp the characteristics of cells and make biological decisions such as diagnoses.

[0081] For example, red blood cells in blood that have become more rigid due to the effect of glycated hemoglobin take longer to pass through a passage of the same width, and the particle measuring device of this embodiment can determine the degree of glycation of individual red blood cells using the proportional relationship between the passage time and the glycated hemoglobin value (HbA1C level).

[0082] Also, referring to Figures 7 and 8, the fluid is formed with a fine electrode pattern, and since the distance d between the electrodes can be calculated in advance, it is also possible to calculate the passing speed of the electrodes from the wavelength b of the waveform.

[0083] As described above, the measuring unit 130 according to this embodiment can count the number of particles using the characteristics of the DC signal output from the filter 138, and can also classify particles based on their inherent properties using the magnitude and length of the signal.

[0084] Hereinafter, the particle measurement method according to this embodiment will be described with reference to FIG.

[0085] Referring to FIG. 9, in order to apply a voltage to a bridge circuit that is in a predetermined electrical state and to a plurality of electrodes that are sequentially arranged on a microchannel through which a fluid passes, an electrical signal is converted into an AC signal first, and then the converted AC signal is amplified.

[0086] Next, the amplified electrical signal is adjusted to a predetermined offset in order to transform the signal into a sine wave-like form (S200).

[0087] The above pre-processed input signal is applied to a bridge circuit (S300).

[0088] Next, the change in the electrical state of the bridge circuit caused by the particles in the fluid passing through the microchannel is measured from the output electrical signal (S400). Specifically, for measurement, only the bouncing signal caused by the difference in the properties of the particles and the solution can be extracted from a portion of the output signal output from the bridge circuit and amplified.

[0089] By removing specific frequency signals from the amplified output signal and finally converting it to direct current, it is possible to detect minute signals generated when particles are positioned between electrodes lined up in the flow path, and count the number of particles in the fluid by the number of times the detected signal occurs.

[0090] It is also possible to calculate the size of particles in the fluid from the magnitude of the phase-shifted electrical signal, and to calculate the transit time from the amplitude of the electrical signal.

[0091] As described above, according to the present invention, minute particles in a solution can be accurately measured using changes in an electrical signal.

[0092] Additionally, the magnitude and time of change in the electrical signal can be used to more accurately measure the properties of the microscopic particles.

[0093] Furthermore, the present invention makes it possible to efficiently measure particle characteristics even at a relatively low voltage using a simple circuit configuration.

[0094] The change in the physical properties of the particles due to glycation of the particles can be used to easily measure the degree of glycation.

[0095] In addition, by calculating the hardness of each individual particle based on the time it takes to pass through a fine flow path, the degree of glycation can be measured more stably and without external or human factors, compared to measurement equipment using biochemical techniques.

[0096] Furthermore, the present invention makes it possible to recognize minute electrical changes caused by the passage of particles using a simple circuit configuration, thereby enabling the degree of glycation of the particles to be measured.

[0097] Furthermore, the present invention can be directly used for clinical diagnosis by correcting the measured particle count using an individual's reference value.

[0098] Furthermore, the various embodiments described herein may be implemented, for example, using software, hardware, or a combination of both in a computer or similar device readable recording medium.

[0099] According to a hardware implementation, the embodiments described herein may be implemented using at least one of application specific integrated circuits (ASICs), digital signal processors (DSPs), digital signal processing devices (DSPDs), programmable logic devices (PLDs), field programmable gate arrays (FPGAs), processors, controllers, micro-controllers, microprocessors, or other functionally performing electrical units. In some cases, the embodiments described herein may be implemented as a control module itself.

[0100] According to a software implementation, the embodiments such as procedures and functions described herein can be implemented in separate software modules, each of which can perform one or more functions and operations described herein. The software code can be implemented in a software application written in a suitable programming language. The software code can be stored in a memory module and executed by a control module.

[0101] The above description is merely illustrative of the technical ideas of the present invention, and various modifications, variations and substitutions can be made by those having ordinary knowledge in the technical field to which the present invention pertains without departing from the essential characteristics of the present invention.

[0102] Therefore, the embodiments and the accompanying drawings disclosed in the present invention are for the purpose of explanation, not for the purpose of limiting the technical idea of ​​the present invention, and the scope of the technical idea of ​​the present invention is not limited by such embodiments and the accompanying drawings. The scope of protection of the present invention should be interpreted according to the following claims, and all technical ideas within the scope equivalent thereto should be interpreted as being included in the scope of the present invention.

Claims

1. A power supply unit that applies a voltage; a chip on which a plurality of electrodes are arranged in sequence on a microchannel through which a fluid passes; a circuit section on which at least a part of a bridge circuit is printed so as to be in a predetermined electrical state with a plurality of electrodes formed on the chip; The device for measuring particles in a solution, characterized in that the power supply unit includes a measurement unit that applies a voltage to the circuit unit and measures a change in the output signal of the bridge circuit due to particles in a fluid passing through the microchannel.

2. 2. The device for measuring particles in a solution according to claim 1, characterized in that the measurement unit detects a phase change in the electrical signal caused by the particle being located between the arranged electrodes, and counts the particles in the fluid by the number of phase changes.

3. 3. The apparatus for measuring particles in a solution according to claim 2, wherein the measurement unit calculates the size of the particles in the fluid from the magnitude of the amplitude of the phase-changed electrical signal.

4. The power supply unit may further include a signal converter that converts an electrical signal applied by the power supply unit into an AC signal, amplifies the AC signal, and adjusts the AC signal to a predetermined offset.

2. The device for measuring particles in a solution according to claim 1, wherein the measurement unit measures the change in the electrical state from an output signal measured in the bridge circuit after the offset-adjusted electrical signal is applied.

5. The device for measuring particles in a solution according to claim 4, characterized in that the measurement unit amplifies the output signal using the offset-adjusted initial electrical signal, removes a specific frequency signal from the amplified output signal to convert it to direct current, and detects a signal due to a change in the electrical state.

6. 6. The apparatus for measuring particles in a solution according to claim 5, wherein the frequency to be removed is set to be the same as or similar to the frequency of the original electrical signal.

7. 6. The apparatus for measuring particles in a solution according to claim 5, wherein the measurement section calculates a time taken for the particle to pass through the flow path by using a generation period of the DC output signal.

8. applying a voltage to a plurality of electrodes arranged in sequence on a microchannel through which a fluid passes and to a bridge circuit that is in a predetermined electrical state; and measuring a change in an output signal of the bridge circuit due to particles in the fluid passing through the microchannel.

9. The measuring step detects a phase change of the electrical signal caused by the particle being positioned between the aligned electrodes, 9. The method for measuring particles in a solution according to claim 8, further comprising the step of counting the number of particles in the fluid based on the number of phase changes.

10. The method for measuring particles in a solution according to claim 9, characterized in that the measuring step calculates the size, structural and electrical properties of the particles in the fluid based on the magnitude of the phase-changed electrical signal.

11. The step of applying a voltage includes: converting the electrical signal into an AC signal and then amplifying the converted AC signal; adjusting the amplified electrical signal to a predetermined offset; 9. The method for measuring particles in a solution according to claim 8, further comprising the step of: applying the offset-adjusted electrical signal to the bridge circuit.

12. The measuring step includes: calculating a difference between the output signals output from the bridge circuit and amplifying the difference; multiplying and amplifying the difference amplified signal with the offset adjusted first electrical signal; The method for measuring particles in a solution according to claim 11, further comprising a step of removing a specific frequency signal from the multiplied and amplified signal to convert it to a direct current.

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