Composition for improving scalp thinning

Abstract

An object of the present invention is to provide a composition capable of improving scalp thinning due to aging, in particular, thinning of the fat layer.SOLUTION: The present invention is a composition for improving scalp thinning, comprising an agent for activating adipose-derived stem cells. The activation of the adipose-derived stem cells is any of promotion of collagen production, promotion of proliferation, and promotion of migration of the adipose-derived stem cells. The fat-derived stem cell activator preferably contains a hydrolyzed protein and / or a synthetic peptide.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a composition for improving scalp thinning. [Background technology]

[0002] Hair problems caused by aging include an increase in gray hair, loss of elasticity and strength, thinning hair (loss of hair volume), and wavy hair. Traditionally, the use of coloring agents and hair straightening have been the main methods used to address these hair problems and maintain the beautiful appearance of hair. However, these traditional methods are not fundamental solutions, and because they generally involve the use of chemicals that chemically alter the proteins that make up hair, they can damage the hair and scalp, potentially leading to a decrease in hair strength, split ends and breakage, and scalp inflammation.

[0003] Age-related changes in scalp hair are known to be caused by factors such as the shrinking of hair follicles in the scalp and a decrease in hair follicle stem cells (Patent Document 1). It is also known that aging causes the scalp's fat layer to thin, disrupting the hair growth cycle (Non-Patent Document 1). As an approach to fundamentally addressing these age-related changes in scalp hair, for example, Patent Document 2 describes how the use of a hair appearance-improving composition containing a specific peptide can improve the deterioration of hair volume and quality (Patent Document 2). Furthermore, Patent Document 3 describes that peony extract, moutan peel extract, and lily extract are effective in preventing or improving age-related hair wavyness. However, these effects are still insufficient, and new, more effective solutions are strongly desired. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2009-161509 [Patent Document 2] Japanese Patent Publication No. 2023-119067 [Patent Document 3] Japanese Patent Application Publication No. 2024-063294 [Non-patent literature]

[0005] [Non-Patent Document 1] Hiroyuki Hori,et al.,J.Investigative Dermatology,vol.58,No.6,pp.396-399 Summary of the Invention [Problem to be solved by the invention]

[0006] Under such circumstances, an object of the present invention is to provide a composition that can improve the thinning of the scalp, particularly the fat layer, which thins with age. [Means for solving the problem]

[0007] As a result of intensive research to solve the above problems, the present inventors have discovered that activating adipose-derived stem cells present in the fat layer of the scalp, i.e., promoting collagen production, proliferation, and migration of adipose-derived stem cells, ultimately leads to improvement in thinning of the scalp fat layer. Specifically, by using a composition containing components that promote collagen production, proliferation, and migration of adipose-derived stem cells present in the fat layer of the scalp, it is possible to improve the thinning of the scalp, particularly the fat layer, which thins with age, and to care for not only existing hair but also hair that will grow in the future. In other words, the gist of the present invention is as follows.

[0008] [1] A composition for improving thinning of the scalp fat layer, containing an adipose-derived stem cell activator. [2] A composition for improving scalp fat layer thinning described in [1], wherein the activation of adipose-derived stem cells is achieved by promoting collagen production, proliferation, or migration of adipose-derived stem cells. [3] A composition for improving scalp fat layer thinning described in [1] or [2], wherein the adipose-derived stem cell activator contains hydrolyzed protein and / or synthetic peptide. [4] A composition for improving scalp fat layer thinning described in [1] or [2], wherein the adipose-derived stem cell activator contains one or more of tripeptide-1 copper, hydrolyzed soybean protein, hydrolyzed white lupin protein, hydrolyzed keratin, and caprooyl tetrapeptide-3. [Effects of the Invention]

[0009] According to the present invention, by activating adipose-derived stem cells present in the fat layer of the scalp, i.e., by promoting collagen production, proliferation, and migration of adipose-derived stem cells, thinning of the scalp fat layer can be improved. Specifically, by using a composition according to the present invention containing a collagen production promoter, a proliferation promoter, and a migration promoter for cells such as adipose-derived stem cells present in the fat layer of the scalp, thinning of the scalp, particularly the fat layer, which thins with age, can be improved. This can improve scalp thinning by plumping up the scalp fat layer. By improving the thinning of the scalp fat layer in this way, it is possible to correct disruptions in the hair growth cycle and care for not only existing hair but also future hair, thereby restoring beautiful, lustrous hair. [Brief explanation of the drawings]

[0010] [Figure 1] FIG. 1 shows the results of an investigation into the collagen production promoting effects of various materials on human adipose-derived stem cells. [Figure 2] FIG. 1 shows the results of an investigation into the proliferation-promoting effects of various materials on human adipose-derived stem cells. DETAILED DESCRIPTION OF THE INVENTION

[0011] The present invention will be described in detail below. The terms used in this specification are to be interpreted in the sense commonly used in the art unless otherwise specified.

[0012] <Composition for improving scalp thinning> The composition for improving scalp thinning of the present invention (hereinafter simply referred to as "the composition of the present invention") is characterized by containing an adipose-derived stem cell activator. In the present invention, the adipose-derived stem cell activator has the effect of promoting collagen production, proliferation, or migration of adipose-derived stem cells. By applying the composition of the present invention to the scalp, it acts on adipose-derived stem cells present in the fat layer of the scalp, promoting one or more of collagen production, proliferation, and migration, and effectively increasing the thickness of the scalp fat layer, thereby improving the thinning of the scalp fat layer. The composition of the present invention is expected to have the effects of improving scalp sagging, scalp thinning (scalp thinning improvement), and providing scalp firmness, so the composition for improving scalp thinning of the present invention is also referred to as, for example, a "scalp thinning improvement composition," a "scalp thinning improvement composition," a "scalp thinning improvement composition," a "scalp thinning improvement composition," a "scalp firmness improvement composition," and the like.

[0013] The scalp thinning improving composition of the present invention can contain, as an adipose-derived stem cell activator, a component that promotes collagen production, a component that promotes proliferation, and a component that promotes migration of adipose-derived stem cells. In addition to the above components, the scalp thinning improving composition of the present invention may contain other optional components to further enhance the effects of the present invention, as long as the effects of the present invention are not impaired. The scalp thinning improving composition of the present invention will be described in detail below.

[0014] [Adipose-derived stem cells] The adipose-derived stem cells on which the composition of the present invention acts are not particularly limited as long as they are mesenchymal stem cells (stromal cells) present in adipose tissue, but adipose-derived stem cells present in the scalp are preferred, and from the viewpoint of the effects of the present invention, adipose-derived stem cells present in the adipose layer of the scalp are more preferred. When collagen production in adipose-derived stem cells is promoted, the produced collagen serves as a scaffold for cell proliferation, thereby promoting the proliferation of adipose-derived stem cells. In addition, adipose-derived stem cells can also proliferate through the direct action of the composition of the present invention. Furthermore, when the migration of adipose-derived stem cells is promoted by the composition of the present invention, it is thought that space is formed, thereby promoting cell proliferation.

[0015] The adipose-derived stem cells are abundant in the adipose layer in the so-called subcutaneous tissue, below the dermis layer of the scalp, and like other stem cells, have the ability to self-renew and differentiate. They are also called adipose-derived mesenchymal stem cells, adipose tissue-derived mesenchymal stem cells, adipose tissue-derived stromal cells, or adipose tissue-derived stromal cells.

[0016] Adipose-derived stem cells are cells that have the ability to differentiate into one or more types of mesenchymal cells (adipocytes, bone cells, cardiac muscle cells, chondrocytes, tenocytes, etc.) and can proliferate while maintaining this ability. Adipose-derived stem cells are cells that become adipocytes in the adipose layer, which have large lipid droplets in their cytoplasm. In the present invention, the adipose-derived stem cells are mammalian adipose-derived stem cells to which the composition for improving scalp thinning can be applied, preferably human, non-human primate, horse, cow, sheep, pig, dog, cat, rabbit, mouse, rat, etc., and particularly preferably human adipose-derived stem cells.

[0017] [Adipose-derived stem cell activation] In the present invention, activation of adipose-derived stem cells refers to any one of promotion of collagen production, promotion of proliferation, and promotion of migration of adipose-derived stem cells.

[0018] [Adipose-derived stem cell activator] The adipose-derived stem cell activator contained in the composition of the present invention refers to a composition capable of activating adipose-derived stem cells, and in the present invention, refers to a composition that has the effect of promoting collagen production, proliferation, or migration of adipose-derived stem cells. Among the above-mentioned adipose-derived stem cell activators that have the effect of promoting collagen production, proliferation, or migration, a composition that has any two of the effects of promoting collagen production, proliferation, and migration is preferred, and a composition that has all three effects is most preferred. The above-mentioned adipose-derived stem cell activator preferably contains a hydrolyzed protein and / or a synthetic peptide as a component that activates adipose-derived stem cells.

[0019] [Hydrolyzed Protein] Examples of hydrolyzed proteins contained in the adipose-derived stem cell activator of the present invention include hydrolyzed white lupin protein, hydrolyzed soybean protein, hydrolyzed keratin, hydrolyzed collagen, hydrolyzed silk, hydrolyzed casein, hydrolyzed milk protein, hydrolyzed conchillion, hydrolyzed wheat, hydrolyzed pea protein, and hydrolyzed rice protein, with hydrolyzed white lupin protein, hydrolyzed soybean protein, and hydrolyzed keratin being preferred.

[0020] When the composition of the present invention contains a hydrolyzed protein as an adipose-derived stem cell activator, the content of the hydrolyzed protein in the composition of the present invention is preferably 0.0001% by weight, more preferably 0.001% by weight or more, and even more preferably 0.01% by weight or more, based on the total weight of the composition. Also, the content of the hydrolyzed protein in the composition of the present invention is 25% by weight or less, preferably 20% by weight or less, more preferably 15% by weight or less, even more preferably 10% by weight or less, particularly preferably 5% by weight or less, and even more particularly preferably 3% by weight or less.

[0021] The content of hydrolyzed protein in the composition of the present invention is 0.0001% to 25% by weight based on the total weight of the composition, and from the viewpoint of the effects of the present invention, it is preferably 0.001% to 20% by weight, more preferably 0.001% to 15% by weight, even more preferably 0.001% to 10% by weight, particularly preferably 0.001% to 5% by weight, and even more particularly preferably 0.01% to 3% by weight. When two or more types of hydrolyzed protein are used in combination, the total amount thereof should be within the above-mentioned range.

[0022] [Synthetic peptides] The synthetic peptides contained in the adipose-derived stem cell activator of the present invention include oligopeptides and polypeptides such as dipeptides, tripeptides, tetrapeptides, pentapeptides, and derivatives thereof. Specific examples include copper tripeptide-1, caprooyl tetrapeptide-3 (a reaction product of a tetrapeptide composed of lysine, threonine, and serine with caproic acid), tetrapeptide-5, palmitoyl pentapeptide-7, oligopeptide-24, pentapeptide-48, palmitoyl pentapeptide-18, etc., with copper tripeptide-1 and caprooyl tetrapeptide-3 being preferred.

[0023] When the composition of the present invention contains a synthetic peptide as an adipose-derived stem cell activator, the content of the synthetic peptide in the composition of the present invention is 0.0000001% by weight or more, preferably 0.000001% by weight or more, more preferably 0.00001% by weight or more, even more preferably 0.0001% by weight or more, and even more preferably 0.001% by weight or more, based on the total weight of the composition. Also, the content of the synthetic peptide in the composition of the present invention is 1% by weight or less, preferably 0.5% by weight or less, more preferably 0.3% by weight or less, and even more preferably 0.1% by weight or less.

[0024] The content of the synthetic peptide in the composition of the present invention is 0.0000001 to 1% by weight, preferably 0.000001 to 1% by weight, more preferably 0.00001 to 0.5% by weight, even more preferably 0.0001 to 0.5% by weight, even more preferably 0.001 to 0.5% by weight, and particularly preferably 0.001 to 0.3% by weight, based on the total weight of the composition. When two or more synthetic peptides are used in combination, the total amount should be within the above-mentioned range.

[0025] When the composition of the present invention contains both hydrolyzed protein and synthetic peptide as an adipose-derived stem cell activator, the total content of hydrolyzed protein and synthetic peptide in the composition of the present invention is preferably 0.0001% by weight or more, more preferably 0.001% by weight or more, and even more preferably 0.01% by weight or more, based on the total weight of the composition. Furthermore, the content of hydrolyzed protein in the composition of the present invention is 25% by weight or less, preferably 20% by weight or less, more preferably 15% by weight or less, even more preferably 10% by weight or less, particularly preferably 5% by weight or less, and even more particularly preferably 3% by weight or less.

[0026] Furthermore, the total content of hydrolyzed proteins and synthetic peptides in the composition of the present invention is 0.0001% by weight to 25% by weight relative to the total weight of the composition, and from the viewpoint of the effects of the present invention, it is preferably 0.001% by weight to 20% by weight, more preferably 0.001% by weight to 15% by weight, even more preferably 0.001% by weight to 10% by weight, particularly preferably 0.001% by weight to 5% by weight, and even more particularly preferably 0.01% by weight to 3% by weight.

[0027] The adipose-derived stem cell activator contained in the composition of the present invention preferably contains hydrolyzed protein and / or synthetic peptide, and preferably contains hydrolyzed white lupin protein, tripeptide-1 copper (a copper complex compound of a tripeptide composed of glycine, histidine, and lysine), or caprooyl tetrapeptide-3 (a reaction product of a tetrapeptide composed of lysine, threonine, and serine with caproic acid). Those containing hydrolyzed white lupin protein and tripeptide-1 copper (a copper complex compound of a tripeptide composed of glycine, histidine, and lysine), those containing hydrolyzed white lupin protein and caprooyl tetrapeptide-3 (a reaction product of a tetrapeptide composed of lysine, threonine, and serine with caproic acid), and those containing tripeptide-1 copper (a copper complex compound of a tripeptide composed of glycine, histidine, and lysine) and caprooyl tetrapeptide-3 (a reaction product of a tetrapeptide composed of lysine, threonine, and serine with caproic acid) are more preferred, and those containing hydrolyzed white lupin protein, tripeptide-1 copper (a copper complex compound of a tripeptide composed of glycine, histidine, and lysine) and caprooyl tetrapeptide-3 (a reaction product of a tetrapeptide composed of lysine, threonine, and serine with caproic acid) are particularly preferred.

[0028] In addition to the above-mentioned components, the composition of the present invention may contain the following optional components, provided that the effects of the present invention are not impaired. For example, the composition may contain anti-inflammatory agents, refreshing agents, disinfectants, preservatives, vitamins, organic acids, moisturizing components, scrubbing agents, UV-absorbing components, UV-scattering components, astringent components, cleansing components, keratin softening components, cell activating components, anti-aging components, blood circulation promoting components, whitening components, powders, etc. In the composition of the present invention, these components may be used alone or in combination of two or more.

[0029] [pH] The pH of the composition of the present invention is usually 3.0 to 10.0, and preferably 3.5 to 7.0, which can be adjusted by using a pH adjuster.

[0030] <Method for producing a composition for improving scalp thinning> The method for producing the composition of the present invention is not particularly limited, and the composition can be produced by a conventional method by appropriately selecting and combining the above-mentioned adipose-derived stem cell activator and other components to be added as needed (optional components, bases or carriers, additives, etc., as described below).The composition of the present invention may be an emulsified composition, a solubilized composition, or a dispersed or dissolved composition.

[0031] <Uses of the composition for improving scalp thinning> The composition of the present invention can be used as a product to be applied directly to the scalp by spraying or other methods, or as a cleanser for cleansing the scalp and hair, a hair dye, a hair growth agent, etc. The composition of the present invention used for such purposes can be suitably used, for example, as a scalp care product to be applied directly to the scalp, hair care agents such as shampoo, treatment, color treatment, and dry shampoo, hair coloring agents, etc., and as a formulation form, can be suitably used as an ointment, stick, liquid, suspension, emulsion (milky lotion and cream), gel, liniment, lotion, cataplasm, mist, powder, granules, etc. In particular, the composition of the present invention is preferably used as a scalp care product or hair care agent.

[0032] <Formulation> The composition of the present invention can be prepared in various formulations by mixing the essential components and the other optional components described above with a base or carrier commonly used in cosmetics, pharmaceuticals, and quasi-drugs, and, if necessary, with the additives described below, according to conventional methods, followed by emulsification or solubilization as necessary. The formulation of the composition of the present invention is not particularly limited, and examples include ointments, sticks, solutions, suspensions, emulsions (lotions and creams), gels, liniments, lotions, poultices, mists, aerosols, powders, and granules. Among these, liquid to solid formulations are preferred, and solutions, lotions, ointments, solids, gels, and emulsions are particularly useful. These formulations can be prepared according to conventional methods, such as those described in the General Provisions for Preparations of the Japanese Pharmacopoeia, 18th Edition. [Example]

[0033] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0034] 1. Search for materials that promote collagen production in adipose-derived stem cells Method: Human adipose-derived mesenchymal stem cells (adMSCs) (KURABO, KW-4109) were cultured at 0.35 x 10 4 Cells were seeded onto a 96-well plate using StemLife (KURABO, LWC-LL0034) at 100 cells / well and cultured for 24 hours. After 24 hours of culture, tripeptide-1 copper, hydrolyzed soy protein, hydrolyzed keratin, or hydrolyzed white lupin protein was added to the final concentrations shown in Figure 1. The cells were then cultured for an additional 72 hours, and the culture supernatant was collected. The culture conditions were 37°C and 5% CO2. The amount of procollagen type I C-terminal peptide was measured using the culture supernatant with a Procollagen Type I C-Peptide (PIP) EIA Kit (TaKaRa, MK101). The results are shown in Figure 1. The amount of procollagen type I C-terminal peptide in the absence of any additives was used as the control, and values ​​are expressed relative to the control (100%). (*P<0.05, **P<0.01, ***P<0.001)

[0035] Results: As shown in Figure 1, the addition of tripeptide 1-copper, hydrolyzed soy protein, hydrolyzed keratin, and hydrolyzed white lupin protein increased the amount of procollagen type II secreted by adipose-derived mesenchymal stem cells.

[0036] 2. Search for materials that promote the proliferation of adipose-derived stem cells Method: Human adipose-derived mesenchymal stem cells (adMSCs) (KURABO, KW-4109) were cultured at 0.35 x 10 4 Cells were seeded onto a 96-well plate using StemLife (KURABO, LWC-LL0034) at 100 cells / well and cultured for 24 hours. Then, tripeptide 1-copper, hydrolyzed soy protein, hydrolyzed keratin, or hydrolyzed white lupin protein was added to the final concentrations shown in Figure 2. The cells were then cultured for an additional 72 hours, and proliferation activity was measured using a Cell Counting Kit (Dojindo, CCK-8). Culture conditions were 37°C and 5% CO2. The results are shown in Figure 2. The proliferation activity without any addition was used as the control, and the control was used as the baseline (100%). (*P<0.05, **P<0.01, ***P<0.001)

[0037] Results: As shown in Figure 2, the addition of tripeptide 1-copper, hydrolyzed soy protein, hydrolyzed keratin, or hydrolyzed white lupin protein promoted the proliferation of adipose-derived mesenchymal stem cells.

[0038] Applying a composition containing tripeptide 1-copper, hydrolyzed white lupine protein, and caprooyl tetrapeptide-3 to the scalp acts on adipose-derived stem cells in the scalp's fat layer, promoting collagen production, proliferation, and migration from the adipose-derived stem cells, which is expected to improve the thinning of the scalp fat layer and make the scalp plumper. [Industrial Applicability]

[0039] According to the present invention, by promoting collagen production, proliferation, etc. of cells such as adipose-derived stem cells present in the fat layer of the scalp, it is possible to improve thinning of the scalp fat layer. Specifically, by using a composition according to the present invention containing an adipose-derived stem cell activator that activates adipose-derived stem cells present in the fat layer of the scalp, it is possible to improve the thinning of the scalp, which thins with age, particularly the fat layer, and to care for not only existing hair but also hair that will grow in the future.

Claims

1. A composition for improving scalp fat layer thinning, comprising an adipose-derived stem cell activator.

2. 2. The composition for improving scalp fat layer thinning according to claim 1, wherein the activation of adipose-derived stem cells is achieved by promoting collagen production, proliferation, or migration of adipose-derived stem cells.

3. The composition for improving scalp fat layer thinning according to claim 1 or 2, wherein the adipose-derived stem cell activator contains a hydrolyzed protein and / or a synthetic peptide.

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