Water-soluble unit dose article

Abstract

To provide a detergent article providing improved malodor removal even when used in a short cycle at a low water temperature.SOLUTION: A water-soluble unit dose detergent article comprising a water-soluble film and a detergent composition, wherein the water-soluble film comprises bacterial spores.SELECTED DRAWING: None

Description

[Technical Field]

[0001] Water-soluble unit dose detergent articles and methods of making and using the articles of the present invention. [Background technology]

[0002] Malodor on fabrics can be a problem with the current trend toward using lower wash temperatures and shorter cycles. It is an object of the present invention to provide a detergent article that provides improved malodor removal even when used at low water temperatures and short cycles. The detergent article should be stable throughout its life, i.e., during transportation, storage, and in the consumer's home. Summary of the Invention [Means for solving the problem]

[0003] According to a first aspect of the present invention, there is provided a water-soluble unit-dose article. The water-soluble unit-dose article comprises a water-soluble film and a detergent composition. The detergent composition is enclosed in the water-soluble film. The detergent composition comprises a surfactant, and the water-soluble film comprises bacterial spores.

[0004] According to a second aspect of the present invention, there is provided a method for producing a water-soluble unit dose detergent article of the present invention, comprising the steps of: a) forming a film containing bacterial spores; and b) encapsulating the detergent composition in a water-soluble film.

[0005] According to a third aspect of the present invention, there is provided a method of disinfecting fabrics using the water-soluble unit dose article of the present invention.

[0006] The water-soluble unit dose article is stable for its lifetime and provides good fabric malodor removal and / or fabric malodor prevention. DETAILED DESCRIPTION OF THE INVENTION

[0007] As used herein, the articles including "the," "a," and "an," when used in a claim or the specification, are understood to mean one or more of what is claimed or described.

[0008] As used herein, the terms "include", "includes" and "including" are meant to be open-ended.

[0009] All percentages, ratios, and proportions used herein are by weight of the composition unless otherwise specified. All average values ​​are calculated "by weight" of the composition unless expressly indicated otherwise.

[0010] Unless otherwise specified, all measurements are performed at 25°C.

[0011] Unless otherwise noted, all ingredient or composition concentrations are in terms of the active portion of that ingredient or composition and are exclusive of impurities, e.g., residual solvents or by-products, that may be present in commercial sources of such ingredient or composition.

[0012] Unit quantity items The present invention relates to a unit dose article. The unit dose article includes a water-soluble film that contains an interior volume enclosed by the water-soluble film. The interior volume contains a liquid composition of the present invention.

[0013] Multi-compartment unit dose articles, also referred to herein as multi-compartment, contain two or more compartments.

[0014] The water-soluble film seals the composition so that it does not leak from the compartment during storage. However, when the water-soluble unit-dose article is added to water, the water-soluble film dissolves, releasing the contents of the inner compartment into the wash solution.

[0015] Each compartment should be understood to mean a closed internal space within the unit-dose article that holds the composition. The unit-dose article is manufactured so that the water-soluble film completely surrounds the composition, thereby defining the compartment in which the composition resides. The film is described in more detail below.

[0016] A unit portion article may include one or more sections, two or more, or even at least three compartments, or even at least four compartments, preferably at least two compartments in a nested position, i.e., positioned one on top of the other. The unit portion article may further include compartments oriented in a side-by-side orientation, i.e., oriented one next to the other, or may even be oriented in a "tire and rim" configuration, i.e., a first compartment is positioned next to a second compartment, but the first compartment at least partially surrounds but does not completely surround the second compartment, or one compartment may be completely enclosed within another compartment.

[0017] One of the compartments may be smaller than the other compartments. If the unit portion article includes at least three compartments, two of the compartments may be smaller than the third compartment, preferably with the smaller compartment stacked on top of the larger compartment. If the unit portion article includes at least four compartments, three of the compartments may be smaller than the fourth compartment, preferably with the smaller compartment stacked on top of the larger compartment. The stacked compartments are preferably oriented side-by-side.

[0018] The water-soluble film includes an inner surface that contacts the composition and an outer surface that is oriented away from the composition and toward the external environment.

[0019] Preferably, the water-soluble unit-dose article comprises one larger compartment overlaid with at least one, preferably at least two, or even at least three smaller compartments, and preferably a first composition is contained in the smaller compartment (first compartment) and a second composition is contained in the larger compartment (second compartment).

[0020] The outer contour seal area includes, or preferably consists of, a flange area. The flange area is disposed around the periphery of the unit-portion article, and the flange comprises a sealed film from at least two, three, or more water-soluble films. In other words, the flange area comprises a film that protrudes outward from the unit-portion article and is sealed. As used herein, "seal area" refers to both the inner seal area, defined as the area of ​​films sealed together to define individual compartments where no flange is present, and the outer seal area, which defines the flange of the water-soluble unit-portion article. Here, the flange excludes the inner seal area. Preferably, the flange comprises films sealed from at least a first water-soluble film, a second water-soluble film, and a third water-soluble film. The inner seal area can be created by sealing two water-soluble films together to create physically separated individual compartments, or by sealing at least three films together to create physically separated individual compartments. Preferably, the inner seal is created by sealing only two water-soluble films together.

[0021] Water-soluble film The films of the unit dose articles of the present invention are water-soluble or water-dispersible. The water-soluble films preferably have a pre-deformation thickness of 20 to 150 micrometers, preferably 35 to 125 micrometers, even more preferably 50 to 110 micrometers, and most preferably about 76 micrometers.

[0022] Preferably, the film is at least 50%, preferably at least 75%, or even at least 95% water soluble when measured by the methods described herein after use of a glass filter having a maximum pore size of 20 micrometers.

[0023] 5 grams ± 0.1 grams of film material was added to a pre-weighed 3 L beaker, followed by 2 L ± 5 mL of distilled water. This was vigorously stirred at 30 °C for 30 minutes using a magnetic stirrer (Labline Model No. 1250 or equivalent) set at 600 rpm and a 5 cm magnetic stirrer. The mixture was then filtered through a pleated, tempered glass filter with a pore size (maximum 20 micrometers) as defined above. The water was dried off from the collected filtrate by any conventional method, and the weight of the remaining material was determined (this is the dissolved or dispersed fraction). The percentage of solubility or dispersion can then be calculated.

[0024] The preferred film material is preferably a polymeric material and can be obtained by methods well known in the art, for example by casting, blow molding, extrusion or blow extrusion of polymeric materials.

[0025] The water-soluble film comprises a polyvinyl alcohol polymer, the polyvinyl alcohol polymer comprising a polyvinyl alcohol homopolymer, an anionic polyvinyl alcohol copolymer, or a blend thereof, preferably the anionic polyvinyl alcohol copolymer is selected from sulfonated and carboxylated anionic polyvinyl alcohol copolymers, especially carboxylated anionic polyvinyl alcohol copolymers. Most preferably, the water-soluble film comprises a blend of polyvinyl alcohol homopolymers, a blend of polyvinyl alcohol homopolymers and carboxylated anionic polyvinyl alcohol copolymers, or the polyvinyl alcohol consists of an anionic polyvinyl alcohol copolymer, most preferably a carboxylated anionic polyvinyl alcohol copolymer, or the polyvinyl alcohol comprises a polyvinyl alcohol homopolymer. When the polyvinyl alcohol in the water-soluble film is a blend of a polyvinyl alcohol homopolymer and a carboxylated anionic polyvinyl alcohol copolymer, the homopolymer and the anionic copolymer are present in a relative weight ratio of 90 / 10 to 10 / 90, preferably 80 / 20 to 20 / 80, and more preferably 70 / 30 to 50 / 50. General classes of anionic monomer units that can be used in anionic polyvinyl alcohol polymers include vinyl polymerized units corresponding to monocarboxylic acid vinyl monomers, their esters and anhydrides, dicarboxylic acid monomers with a polymerizable double bond, their esters and anhydrides, vinyl sulfonic acid monomers, and alkali metal salts of any of the foregoing.Examples of suitable anionic monomer units include vinyl polymerized units corresponding to vinyl anionic monomers, such as vinyl acetic acid, maleic acid, monoalkyl maleates, dialkyl maleates, monomethyl maleate, dimethyl maleate, maleic anhydride, fumaric acid, monoalkyl fumarate, dialkyl fumarate, monomethyl fumarate, dimethyl fumarate, fumaric anhydride, itaconic acid, monomethyl itaconate, dimethyl itaconate, itaconic anhydride, vinyl sulfonic acid, allyl sulfonic acid, ethylene sulfonic acid, 2-acrylamido-1-methylpropanesulfonic acid, 2-acrylamido-2-methylpropanesulfonic acid, 2-methylacrylamido-2-methylpropanesulfonic acid, 2-sulfoethyl acrylate (2-sulfoethyl acrylate), Examples of anionic monomers include 2-acrylamidomethylpropanesulfonic acid (e.g., 2-acrylamido-1-methylpropanesulfonic acid, 2-acrylamido-2-methylpropanesulfonic acid, 2-methylacrylamido-2-methylpropanesulfonic acid), alkali metal salts thereof (e.g., sodium salts, potassium salts, or other alkali metal salts), esters of the above (e.g., methyl, ethyl, or other C1-C4 or C6 alkyl esters), and combinations thereof (e.g., multiple types of anionic monomers, or equivalent forms of the same anionic monomer). The anionic monomer may be one or more of acrylamidomethylpropanesulfonic acid (e.g., 2-acrylamido-1-methylpropanesulfonic acid, 2-acrylamido-2-methylpropanesulfonic acid), alkali metal salts thereof (e.g., sodium salts), and combinations thereof. Preferably, the anionic portion of the first anionic monomer unit is selected from sulfonates, carboxylates, or mixtures thereof, more preferably carboxylates, and most preferably acrylates, methacrylates, maleates, or mixtures thereof. Preferably, the anionic monomer units are present in the anionic polyvinyl alcohol copolymer in an average amount ranging from 1 mol % to 5 mol %, preferably from 2 mol % to 5 mol %.

[0026] The polyvinyl alcohol polymer may be present in an amount of 50% to 95% by weight, preferably 55% to 90% by weight, and more preferably 60% to 80% by weight, based on the weight of the water-soluble film.

[0027] Without wishing to be bound by theory, the term "homopolymer" generally includes polymers having one type of monomer repeat unit (e.g., a polymer chain comprising or consisting of a single monomer repeat unit). In the case of polyvinyl alcohol in particular, the term "homopolymer" also includes copolymers having a distribution of vinyl alcohol monomer units and optionally vinyl acetate monomer units (e.g., a polymer chain comprising or consisting of vinyl alcohol monomer units and vinyl acetate monomer units), depending on the degree of hydrolysis. When hydrolysis is 100%, a polyvinyl alcohol homopolymer may contain only vinyl alcohol units. Without wishing to be bound by theory, the term "copolymer" generally includes polymers having two or more types of monomer repeat units (e.g., a polymer chain comprising or consisting of two or more different monomer repeat units, whether a random copolymer, a block copolymer, etc.). In the case of polyvinyl alcohol in particular, the term "copolymer" (or "polyvinyl alcohol copolymer") further includes copolymers having a distribution of vinyl alcohol and vinyl acetate monomer units and at least one other type of monomer repeat unit, depending on the degree of hydrolysis (e.g., ter(or more) polymer chains comprising or consisting of vinyl alcohol, vinyl acetate, and one or more other monomer units, such as anionic monomer units). When hydrolysis is 100%, polyvinyl alcohol copolymers may include copolymers having vinyl alcohol units and one or more other monomer units, but no vinyl acetate units. Without being bound by theory, the term "anionic copolymer" includes copolymers having anionic monomer units that contain anionic moieties.

[0028] Preferably, the polyvinyl alcohol and / or, in the case of polyvinyl alcohol blends, the individual polyvinyl alcohol polymers and / or composite polyvinyl alcohol polymers have an average viscosity (μl) ranging from 4 mPa·s to 30 mPa·s, preferably 10 mPa·s to 25 mPa·s, when measured as a 4% polyvinyl alcohol polymer solution in demineralized water at 20°C. The viscosity of polyvinyl alcohol polymers is determined by measuring freshly prepared solutions using a Brookfield LV viscometer equipped with a UL adapter, as described in British Standard EN ISO 15023-2:2006 Annex E Brookfield Test method. It is international practice to specify the viscosity of a 4% aqueous polyvinyl alcohol solution at 20°C. It is well known in the art that the viscosity of aqueous solutions of water-soluble polymers (polyvinyl alcohol or otherwise) correlates with the weight-average molecular weight of the same polymer, and viscosity is often used as a proxy for weight-average molecular weight. Thus, the weight average molecular weight of the polyvinyl alcohol may be in the range of 30,000 to 175,000, or 30,000 to 100,000, or 55,000 to 80,000. Preferably, in the case of polyvinyl alcohol and / or polyvinyl alcohol blends, the individual polyvinyl alcohol polymers have an average degree of hydrolysis in the range of 75% to 99%, preferably 80% to 95%, and most preferably 85% to 95%. A suitable test method for measuring the degree of hydrolysis is in accordance with the standard method JIS K6726.

[0029] The water-soluble film may comprise a material selected from the group consisting of proteins, polysaccharides, and mixtures thereof. The film may comprise casein, pectin, carrageenan, or mixtures thereof.

[0030] Preferably, the water-soluble film contains a non-aqueous plasticizer. Preferably, the non-aqueous plasticizer is selected from polyols, sugar alcohols, and mixtures thereof. Suitable polyols include glycerol, diglycerin, ethylene glycol, diethylene glycol, triethylene glycol, tetraethylene glycol, polyethylene glycols of 400 molecular weight or less, neopentyl glycol, 1,2-propylene glycol, 1,3-propanediol, dipropylene glycol, polypropylene glycol, 2-methyl-1,3-propanediol, trimethylolpropane, and polyether polyols, or mixtures thereof. Suitable sugar alcohols include sugar alcohols selected from the group consisting of isomalt, maltitol, sorbitol, xylitol, erythritol, adonitol, dulcitol, pentaerythritol, and mannitol, or mixtures thereof. More preferably, the non-aqueous plasticizer is selected from glycerol, 1,2-propanediol, dipropylene glycol, 2-methyl-1,3-propanediol, trimethylolpropane, triethylene glycol, polyethylene glycol, sorbitol, or a mixture thereof, and most preferably from glycerol, sorbitol, trimethylolpropane, dipropylene glycol, and a mixture thereof. One particularly suitable plasticizer system comprises a blend of glycerol, sorbitol, and trimethylolpropane. Another particularly suitable plasticizer system comprises a blend of glycerol, dipropylene glycol, and sorbitol. Preferably, the film comprises 5% to 50%, preferably 10% to 40%, more preferably 20% to 30% by weight of the film of the non-aqueous plasticizer.

[0031] Preferably, the water-soluble film contains a surfactant. Preferably, the water-soluble film contains 0.1% to 2.5% by weight of the surfactant, preferably 1% to 2% by weight of the surfactant. Suitable surfactants include nonionic, cationic, anionic, and zwitterionic surfactants. Suitable surfactants include, but are not limited to, polyoxyethylenated polyoxypropylene glycols, alcohol ethoxylates, alkylphenol ethoxylates, tertiary acetylenic glycols and alkanolamides (nonionics), polyoxyethylenated amines, quaternary ammonium salts, and quaternized polyoxyethylenated amines (cationic materials), and amine oxides, N-alkylbetaines, and sulfobetaines (zwitterionic materials). Other suitable surfactants include dioctyl sodium sulfosuccinate, lactylated fatty acid esters of glycerol and propylene glycol, lactylic esters of fatty acids, sodium alkyl sulfate, polysorbate 20, polysorbate 60, polysorbate 65, polysorbate 80, lecithin, acetylated fatty acid esters of glycerol and propylene glycol, and acetylated esters of fatty acids, and combinations thereof.

[0032] Preferably, the water-soluble film according to the present invention contains a lubricant / release agent. Suitable lubricants / release agents include, but are not limited to, fatty acids and their salts, fatty alcohols, fatty acid esters, fatty amines, fatty amine acetates, and fatty acid amides. Preferred lubricants / release agents are fatty acids, fatty acid salts, and fatty amine acetates. Preferably, the amount of lubricant / release agent in the water-soluble film ranges from 0.02% to 1.5% by weight, preferably from 0.1% to 1% by weight, of the water-soluble film.

[0033] Preferably, the water-soluble film contains a filler, a spreading agent, an anti-blocking agent, a detackifying agent, or a mixture thereof. Suitable fillers, spreading agents, anti-blocking agents, detackifying agents, or mixtures thereof include, but are not limited to, starch, modified starch, cross-linked polyvinylpyrrolidone, cross-linked cellulose, microcrystalline cellulose, silica, metal oxides, calcium carbonate, talc, and mica. Preferred materials are starch, modified starch, and silica. Preferably, the amount of filler, spreading agent, anti-blocking agent, detackifying agent, or mixtures thereof in the water-soluble film ranges from 0.1% to 25% by weight of the water-soluble film, preferably from 1% to 10% by weight, more preferably from 2% to 8% by weight, and most preferably from 3% to 5% by weight. In the absence of starch, one preferred range for a suitable filler, spreading agent, anti-blocking agent, de-tackifying agent, or mixture thereof is 0.1% to 1%, preferably 4%, more preferably 6%, even more preferably 1% to 4%, and most preferably 1% to 2.5% by weight of the water-soluble film.

[0034] Preferably, the water-soluble films according to the present invention have a residual moisture content, as measured by Karl Fischer titration, of at least 4%, more preferably in the range of 4% to 15%, and even more preferably 5% to 10% by weight of the water-soluble film.

[0035] Preferred films are those that exhibit good solubility in cold water, i.e., unheated distilled water. Preferably, such films exhibit good solubility at a temperature of 24° C., and even more preferably at 10° C. Good solubility means that the film exhibits at least 50%, preferably at least 75%, or even at least 95% water solubility when measured by the method described herein after using a glass filter having a maximum pore size of 20 micrometers as described above.

[0036] The film may be opaque, transparent or translucent. Preferably, the film is transparent. The film may include printed areas.

[0037] The printed areas can be obtained using standard techniques such as flexographic printing or inkjet printing.

[0038] The film may also include an aversive agent, such as a bittering agent. Suitable bittering agents include, but are not limited to, naringin, sucrose octaacetate, quinine hydrochloride, denatonium benzoate, or mixtures thereof. Any suitable concentration of the aversive agent may be used in the film. Suitable concentrations include, but are not limited to, 1 to 5000 ppm, or even 100 to 2500 ppm, or even 250 to 2000 ppm.

[0039] The water-soluble film, the water-soluble unit dose article, or both may be further coated with a powder. The powder preferably has an average particle size of 0.1 to 20 microns, preferably 5 to 15 microns. Preferably, the powder is selected from talc, zinc oxide, silica, siloxane, zeolite, silicic acid, alumina, sodium sulfate, potassium sulfate, calcium carbonate, magnesium carbonate, sodium citrate, sodium tripolyphosphate, potassium citrate, potassium tripolyphosphate, calcium stearate, zinc stearate, magnesium stearate, starch, modified starch, clay, kaolin, gypsum, cyclodextrin, or mixtures thereof. The powder is generally applied at a rate ranging from 0.5 to 10 mg / 100 cm², preferably 5 mg / 100 cm² or less, more preferably 1.25 to 2.5 mg / 100 cm².

[0040] bacterial spores The bacterial spores are located in the film. Preferably, the film has a bacterial spore content of about 1 x 10 by weight of the film. 3 , preferably about 1 x 10 4 ~Approx. 1×10 11 , preferably 1 x 10 10 Contains CFU / g of bacterial spores.

[0041] Bacterial spores are directly attached to fabrics.The bacterial spores of the composition of the present invention can germinate on fabrics.Spores can be activated by heat, for example, the heat generated during the use of fabrics, or the heat provided in washing machines or dryers.Spores can germinate when fabrics are stored and / or used.Malodor precursors can be used by the microorganisms produced by spores as nutrients to promote germination.Spores can germinate after fabrics are left in a wet environment.

[0042] The spores have the ability to germinate and form cells after the fabric has been subjected to a disinfection process. The spores can be provided in liquid or solid form. Preferably, the spores are in solid form.

[0043] Some Gram-positive bacteria have a two-stage life cycle, and bacteria growing under certain conditions, such as in response to nutrient deficiency, can undergo an elaborate developmental process leading to spore or endospore formation. Bacterial spores are protected by a coat composed of approximately 60 different proteins assembled into a biochemically complex structure with interesting morphological and mechanical properties. The protein coat is considered a static structure that provides rigidity and primarily acts as a sieve to filter out large, exogenous, toxic molecules, such as lytic enzymes. Spores play an important role in the long-term survival of species because they are highly resistant to extreme environmental conditions. Spores can also remain metabolically dormant for many years. Methods for obtaining bacterial spores from vegetative cells are well known in the art. In some instances, vegetative bacterial cells are grown in liquid culture. Bacteria can begin spore formation from late logarithmic or early stationary growth phases. Once the bacteria have completed spore formation, the spores can be harvested from the culture medium, for example, by centrifugation. Various methods can be used to sterilize or remove any remaining vegetative cells. Various methods can be used to purify spores from cellular debris and / or other materials or substances. Bacterial spores can be differentiated from vegetative cells using various techniques, for example, phase contrast microscopy, automated scanning microscopy, high resolution atomic force microscopy, or heat resistance methods.

[0044] Bacterial spores are generally metabolically inactive or dormant, environmentally resistant structures that make them easily selected and used in commercial microbial products. Despite their hardiness and extremely long lifespan, spores can rapidly respond to the presence of certain small molecules known as germinants, which signal favorable conditions for spores to break dormancy by germinating, the initial step in the process of completing their life cycle by reverting to vegetative bacteria. For example, commercial microbial products can be designed so that spores are dispersed into an environment where they come into contact with germinants present in the environment, germinate into vegetative cells, and perform their intended function. A variety of different bacteria can form spores. For example, the following genera: Acetonema, Alkalibacillus, Ammoniphilus, Amphibacillus, Anaerobacter, Anaerospora, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Caldanaerobacter, Caloramator, Caminicella, Cerasibacillus, Clostridium, Clostridium salibacter Clostridiisalibacter, Cohnella, Dendrosporobacter, Desulfotomaculum, Desulfosporomusa, Desulfosporosinus, Desulfovirgula, Desulfunispora, Desulfurispora, Filifactor, Filobacillus, Gelria, Geobacillus, Geosporobacter, Gracilibacillus,Halonatronum, Heliobacterium, Heliophilum, Laceyella, Lentibacillus, Lysinibacillus, Mahella, Metabacterium, Moorella, Natroniella, Oceanobacillus, Orenia, Ornithinibacillus, Oxalophagus, Oxobacter, Paenibacillus, Paraliobacillus, Pelospora, Pelotomaculum, Piscibacillus, Planifilum, Pontibacillus, Propionis pora, Salinibacillus, Salsuginibacillus, Seinonella, Shimazuella, Sporacetigenium, Sporoanaerobacter, Sporobacter, Sporobacterium, Sporohalobacter, Sporolactobacillus lactobacillus, Sporomusa, Sporosarcina, Sporotalea, Sporotomaculum, Syntrophomonas, Syntrophospora, Tenuibacillus, Tepidibacter, Terribacillus, Thalassobacillus,Some bacteria, including Thermoacetogenium, Thermoactinomyces, Thermoalkalibacillus, Thermoanaerobacter, Thermoanaeromonas, Thermobacillus, Thermoflavimicrobium, Thermovenablum, Tuberibacillus, Virgibacillus, and / or Vulcanobacillus, can form spores.

[0045] Preferably, the bacterium capable of forming spores is a bacterium of the family Bacillaceae, for example, Aeribacillus, Aliibacillus, Alkalibacillus, Alkalicoccus, Alkalihalobacillus, Alkalilactibacillus, Allobacillus, Alteribacillus, Alteribacter. acter, Amphibacillus, Anaerobacillus, Anoxybacillus, Aquibacillus, Aquisalibacillus, Aureibacillus, Bacillus, Caldalkalibacillus, Calditerricola, Calidifontibacillus, Camelliibacillus, Cerasibacillus, Compostibacillus, Cytobacillus, Desertibacillus, Domibacillus, Ectobacillus, Evansella, Falsibacillus, Ferdinandcohnia, Fermentibacillus, Fictibacillus, Filobacillus, Geobacillus, Geomicrobium, Gottfriedia, Gracilibacillus, Halalkalibacillus, Halobacillus,Halolactibacillus, Heyndrickxia, Hydrogenibacillus, Lederbergia, Lentibacillus, Litchfieldia, Lottiidibacillus, Margalitia, Marinococcus, Melghiribacillus, Mesobacillus, Metabacillus, Microaerobacter, Natribacillus, Natronobacillus, Neobacillus, Niallia, Oceanobacillus, Ornithinibacillus, Parageobacillus, Paraliobacillus O. bacillus, Paralkalibacillus, Paucisalibacillus, Pelagirhabdus, Peribacillus, Piscibacillus, Polygonibacillus, Pontibacillus, Pradoshia, Priestia, Pseudogracilibacillus cillus, Pueribacillus, Radiobacillus, Robertmurraya, Rossellomorea, Saccharococcus, Salibacterium, Salimicrobium, Salinibacillus, Salipaludibacillus, Salirhabdus,Salisediminibacterium, Saliterribacillus, Salsuginibacillus, Sediminibacillus, Siminovitchia, Sinibacillus, Sinobaca, Streptohalobacillus, Sutcliffiella, Swionibacillus, Tenuibacillus, Tepi They are derived from species of the genera Tepidibacillus, Terribacillus, Terriactibacillus, Texcoconibacillus, Thalassobacillus, Thalassorhabdus, Thermolongibacillus, Virgibacillus, Viridibacillus, Vulcanibacillus, and Weizmannia. In various examples, the bacteria include Bacillus achydicola, Bacillus aeolius, Bacillus aerius, Bacillus aerophilus, Bacillus albus, Bacillus alticuzinis, Bacillus albeauensis, Bacillus amyloliquefaciensex, Bacillus anthracis, Bacillus aquiflavi, Bacillus atrophaeus, Bacillus australimalis, Bacillus badius, Bacillus benzoevolans, Bacillus cabriole, alesii, Bacillus canaverarius, Bacillus capparidis, Bacillus carboniphilus, Bacillus cereus, Bacillus chagangensis, Bacillus corefyrensis, Bacillus cytotoxicus, Bacillus decisifrondis, Bacillus ectoiniformans, Bacillus enculensis, Bacillus finchiensis, Bacillus fungorum, Bacillus glitinifermentans, Bacillus gobiensis, Bacillus halotolerans,Bacillus heineshii, Bacillus forti, Bacillus inaquosorum, Bacillus infantis, Bacillus infernus, Bacillus isaberiae, Bacillus quexuae, Bacillus licheniformis, Bacillus luti, Bacillus manusensis, Bacillus marinisedimentorum, Bacillus mesophilus, Bacillus methanolicus, Bacillus mobilis, Bacillus mojavensis, Bacillus mycoides, Bacillus nakamura Bacillus nudiopicus, Bacillus nitrachiredusens, Bacillus oleivorans, Bacillus pacificus, Bacillus pachystanensis, Bacillus paralicheniformis, Bacillus paramycoides, Bacillus parathrasidis, Bacillus pervagus, Bacillus pisticola, Bacillus proteolyticus, Bacillus pseudomycoides, Bacillus pumilus, Bacillus safensis, Bacillus thalassitis, Bacillus sa linus, Bacillus salitollerans, Bacillus theohaeanensis, Bacillus sibazii, Bacillus siamensis, Bacillus smithii, Bacillus solimanglobi, Bacillus sonkurensis, Bacillus sonorensis, Bacillus spizizenii, Bacillus spongiae, Bacillus stercoris, Bacillus stratosphaericus, Bacillus subtilis, Bacillus swithii, Bacillus thaeanensis, Bacillus tamaricis, Bacillus The strain may be a strain of Bacillus techlensis, Bacillus thermocloacae, Bacillus thermotolerans, Bacillus thuringiensis, Bacillus tianchenii, Bacillus toyonensis, Bacillus tropicalis, Bacillus valismortis, Bacillus vereziensis, Bacillus viedmannii, Bacillus vdaliankiensis, Bacillus kiamenensis, Bacillus kiapuensis, Bacillus zhanzhouensis, or a combination thereof.

[0046] In some examples, the spore-forming bacterial strain may be a Bacillus strain, such as Bacillus sp. strain SD-6991, Bacillus sp. strain SD-6992, Bacillus sp. strain NRRL B-50606, Bacillus sp. strain NRRL B-50887, Bacillus pumilus strain NRRL B-50016, Bacillus amyloliquefaciens strain NRRL B-50017, Bacillus amyloliquefaciens strain PTA-7792 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50018, Bacillus amyloliquefaciens strain NRRL B-50019, Bacillus amyloliquefaciens strain NRRL B-50020, Bacillus amyloliquefaciens strain NRRL B-50021, Bacillus amyloliquefaciens strain NRRL B-50022, Bacillus amyloliquefaciens strain NRRL B-50023, Bacillus amyloliquefaciens strain NRRL B-50024, Bacillus amyloliquefaciens strain NRRL B-50025, Bacillus amyloliquefaciens strain NRRL B-50026, Bacillus amyloliquefaciens strain NRRL B-50027, Bacillus amyloliquefaciens strain NRRL B-50028, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-5 B-50018, Bacillus amyloliquefaciens strain PTA-7541, Bacillus amyloliquefaciens strain PTA-7544, Bacillus amyloliquefaciens strain PTA-7545, Bacillus amyloliquefaciens strain PTA-7546, Bacillus subtilis strain PTA-7547, Bacillus amyloliquefaciens strain PTA-7549, Bacillus amyloliquefaciens strain PTA-7793, Bacillus amyloliquefaciens strain PTA-7790, Bacillus amyloliquefaciens strain PTA-7791, Bacillus subtilis strain NRRL B-50136 (also known as DA-33R, ATCC accession number 55406), Bacillus amyloliquefaciens strain NRRL B-50141, Bacillus amyloliquefaciens strain NRRL B-50399, Bacillus licheniformis strain NRRL B-50014, Bacillus licheniformis strain NRRL B-50015, Bacillus amyloliquefaciens strain NRRL B-50607, Bacillus subtilis strain NRRL B-50147 (also known as 300R), Bacillus amyloliquefaciens strain NRRL B-50150, Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus megaterium PTA-3142, Bacillus amyloliquefaciens strain ATCC accession number 55405 (also known as 300), Bacillus amyloliquefaciens strain ATCC accession number 55407 (also known as PMX), Bacillus pumilus NRRL B-50398 (ATCCBacillus cereus ATCC accession number 700385, PMX-1, and NRRL B-50255 (also known as B. cereus ATCC accession number 700386, B. thuringiensis ATCC accession number 700387 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL B-50304 and NRRL B-50349 TAEGRO® available from Novozymes), Bacillus subtilis (e.g., isolate NRRL B-21661 available from Bayer CropScience in RHAPSODY®, SERENADE® MAX, and SERENADE® ASO), Bacillus pumilus ... B-50349 isolate), Bacillus amyloliquefaciens TrigoCor (also known as "TrigoCor 1448", e.g., isolate Embrapa Trigo accession number 144 / 88.4Lev, Cornell accession number Pma007BR-97, and ATCC accession number 202152 available from Cornell University, USA), and combinations thereof.

[0047] In some examples, the spore-forming bacterial strain can be a Bacillus amyloliquefaciens strain. For example, the strain can be Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus) and / or Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50154, or from other Bacillus amyloliquefaciens microorganisms.

[0048] The bacterial spores may have an average particle size of about 2-50 microns, preferably about 10-45 microns. Bacillus spores are commercially available in blends in aqueous carriers, in which they are insoluble. Other commercially available bacillus spore blends include, but are not limited to, Freshen Free™ CAN (10X), available from Novozymes Biologicals, Inc.; Evogen® Renew Plus (10X), available from Genesis Biosciences, Inc.; and Evogen® GT (10X, 20X, and 110X), all available from Genesis Biosciences, Inc. In the foregoing list, the designations in parentheses (10X, 20X, and 110X) indicate the relative concentrations of bacillus spores.

[0049] The bacterial spores used in the compositions, methods, and products disclosed herein may or may not be heat activated. In some instances, the bacterial spores are heat activated. In some instances, the bacterial spores are not heat inactivated. Preferably, the spores used herein are heat activated. Heat activation may involve heating the bacterial spores from room temperature (15-25°C) to an optimum temperature of 25-120°C, preferably 40-100°C, and holding the optimum temperature for up to 2 hours, preferably 70-80°C for 30 minutes.

[0050] For the methods, compositions, and products disclosed herein, a population of bacterial spores is generally used. In some examples, the population of bacterial spores may include bacterial spores from a single strain of bacteria. Preferably, the population of bacterial spores may include bacterial spores from two, three, four, five, or more strains of bacteria. Generally, the population of bacterial spores contains a majority of spores and a small number of vegetative cells. In some examples, the population of bacterial spores does not contain vegetative cells. In some examples, the population of bacterial spores may contain less than about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% vegetative cells, where the percentage of bacterial spores is calculated as ((number of vegetative cells / (number of spores in the population + number of vegetative cells in the population)) × 100). Generally, the populations of bacterial spores used in the disclosed methods, compositions, and products are stable (i.e., not germinating) and at least some individual spores in the population are capable of germination.

[0051] The population of bacterial spores used in the present disclosure may include bacterial spores at different concentrations. In various examples, the population of bacterial spores is at least 1 x 10 2 , 5×10 2 , 1×10 3 , 5×10 3 , 1×10 4 , 5×10 4 , 1×10 5 , 5×10 5 , 1×10 6 , 5×10 6 , 1×10 7 , 5×10 7 , 1×10 8 , 5×10 8 , 1×10 9 , 5×10 9 , 1×10 10 , 5×10 10 , 1×10 11 , 5×10 11 , 1×10 12 , 5×10 12 , 1×10 13 , 5×10 13 , 1×10 14 , or 5 x 10 14This may include, but is not limited to, spores / mL, spores / gram, or spores / cm3.

[0052] Detergent Composition The detergent composition is preferably a laundry detergent composition. The composition may be in the form of a solid, a liquid, or a mixture thereof. Preferably, the composition is a liquid composition.

[0053] The solids may be in the form of free-flowing particulates, compacted solids, or mixtures thereof. It should be understood that the solids may contain some water, but are essentially water-free. In other words, no water is intentionally added other than from the addition of various ingredients.

[0054] In the context of the laundry detergent compositions of the present invention, the term "liquid" encompasses such forms as dispersions, gels, pastes, and the like. Liquid compositions may also contain gases in a suitably divided form. The term "liquid laundry detergent composition" refers to any laundry detergent composition containing a liquid capable of wetting and treating fabrics in a domestic washing machine, e.g., cleaning clothes. A dispersion is, for example, a liquid that contains solid or particulate matter contained therein.

[0055] The laundry detergent composition may include perfume capsules.

[0056] Preferably, the laundry detergent composition comprises a non-soap surfactant, preferably selected from a non-soap anionic surfactant, a non-ionic surfactant, or a mixture thereof. Preferably, the laundry detergent composition comprises from 10% to 60% by weight of the laundry detergent composition of the non-soap surfactant, more preferably from 20% to 55% by weight.

[0057] Preferably, the anionic non-soap surfactant comprises a linear alkyl benzene sulfonate, an alkyl sulfate, an alkoxylated alkyl sulfate, or a mixture thereof. Preferably, the alkoxylated alkyl sulfate is an ethoxylated alkyl sulfate.

[0058] Preferably, the laundry detergent composition comprises from 5% to 60%, preferably from 15% to 55%, more preferably from 25% to 50%, and most preferably from 30% to 45% by weight of the detergent composition of non-soap anionic surfactant.

[0059] Preferably, the non-soap anionic surfactant comprises a linear alkylbenzene sulfonate and an alkoxylated alkyl sulfate, and the weight ratio of the linear alkylbenzene sulfonate to the alkoxylated alkyl sulfate, preferably the linear alkylbenzene sulfonate to ethoxylated alkyl sulfate, is 1:10 to 10:1, preferably 6:1 to 1:6, more preferably 4:1 to 1:4, and even more preferably 4:1 to 1:1. Alternatively, the weight ratio of the linear alkylbenzene sulfonate to the ethoxylated alkyl sulfate is 1:2 to 1:4. The alkoxylated alkyl sulfate can be derived from synthetic or natural alcohols, or blends thereof, with the desired average alkyl carbon chain length and average branching degree. Preferably, the synthetic alcohol is produced according to the Ziegler process, the Oxo process, the modified Oxo process, the Fischer-Tropsch process, the Guerbet process, or a mixture thereof. Preferably, the naturally occurring alcohol is derived from a natural oil, preferably coconut oil, palm kernel oil, or a mixture thereof.

[0060] Preferably, the laundry detergent composition comprises 0% to 30% by weight of the laundry detergent composition, preferably 1% to 25% by weight, more preferably 3% to 20% by weight, and most preferably 5% to 20% by weight of the laundry detergent composition. Preferably, the weight ratio of non-soap anionic surfactant to nonionic surfactant is 1:2 to 20:1, 1:1.5 to 15:1, 1:1 to 10:1, or 1.5:1 to 5:1. The nonionic surfactant is preferably selected from alcohol alkoxylate nonionic surfactants, including naturally occurring alcohols, synthetically derived alcohol-based alcohol alkoxylate nonionic surfactants, and mixtures thereof, depending on the desired average alkyl carbon chain length and average branching degree. The alcohol alkoxylate nonionic surfactant may be a primary or secondary alcohol alkoxylate nonionic surfactant, preferably a primary alcohol alkoxylate nonionic surfactant. Synthetically derived alcohol alkoxylate nonionic surfactants include Ziegler synthesis alcohol alkoxylates, oxo synthesis alcohol alkoxylates, modified oxo process synthesis alcohol alkoxylates, Fischer-Tropsch synthesis alcohol alkoxylates, Guerbet alcohol alkoxylates, alkylphenol alcohol alkoxylates, or mixtures thereof. The alkoxylated chains can be mixed alkoxylated chains containing ethoxy, propoxy, and / or butoxy units, or can be purely ethoxylated alkyl chains, preferably purely ethoxylated alkyl chains.

[0061] Preferably, the laundry detergent composition, preferably a liquid laundry detergent composition, comprises from 1wt% to 20wt% of a soap, preferably a fatty acid salt, more preferably an amine-neutralized fatty acid salt, by weight of the laundry detergent composition, more preferably from 2wt% to 15wt%, even more preferably from 3wt% to 10wt%, and most preferably from 4wt% to 8wt% of a soap, preferably a fatty acid salt, more preferably an amine-neutralized fatty acid salt, preferably wherein the amine is an alkanolamine, more preferably selected from monoethanolamine, diethanolamine, triethanolamine, or mixtures thereof, more preferably monoethanolamine.

[0062] Preferably, the laundry detergent composition comprises a non-aqueous solvent, preferably selected from ethanol, 1,2-propanediol, dipropylene glycol, tripropylene glycol, glycerol, sorbitol, ethylene glycol, polyethylene glycol, polypropylene glycol, or a mixture thereof, preferably the polypropylene glycol having a molecular weight of 400. Preferably, the liquid laundry detergent composition comprises 10% to 40%, preferably 15% to 30%, by weight of the liquid laundry detergent composition of the non-aqueous solvent. Without wishing to be bound by theory, the non-aqueous solvent ensures an appropriate level of film plasticization so that the film is not too brittle or "soft." Without wishing to be bound by theory, having the correct degree of plasticization also facilitates dissolution of the film when exposed to water during the washing process.

[0063] Preferably, the liquid laundry detergent composition comprises from 1% to 20%, preferably from 5% to 15% water by weight of the liquid laundry detergent composition.

[0064] Preferably, the laundry detergent composition comprises an ingredient selected from the list comprising a cationic polymer, a polyester terephthalate polymer, an amphiphilic graft copolymer, an alkoxylated, preferably ethoxylated, polyethyleneimine polymer, carboxymethylcellulose, an enzyme, a bleaching agent, or a mixture thereof.

[0065] Preferably, the laundry detergent composition comprises 0.01% to 5%, more preferably 0.05% to 3%, by weight of the detergent composition, of perfume. A perfume comprises one or more, preferably two or more, perfume raw materials. The term "perfume raw material" (or perfume raw material, "PRM"), as used herein, means a compound having a molecular weight of at least about 100 g / mole and useful for imparting an odor, fragrance, essence, or scent, alone or in combination with other perfume raw materials. Preferably, the detergent perfume of the composition comprises an alcohol, a ketone, an aldehyde, an ester, an ether, a nitrile, and an alkene such as a terpene.

[0066] PRMs may be characterized by their boiling point (BP), measured at atmospheric pressure (760 mmHg), and their octanol / water partition coefficient (P), which may be described in terms of logP, determined according to the test methods set forth in the Test Methods section on page 23 of WO 2022 / 081781. Based on these characteristics, PRMs may be classified as Quadrant I, Quadrant II, Quadrant III, or Quadrant IV fragrances, as described in more detail below. Having various PRMs from different quadrants may be desirable, for example, to provide fragrance benefits at different touchpoints during normal use.

[0067] Perfume raw materials having a boiling point BP below about 250°C and a log P below about 3 are known as Quadrant I perfume raw materials. Quadrant 1 perfume raw materials are preferably limited to less than 30% of the perfume composition. Perfume raw materials having a BP above about 250°C and a log P above about 3 are known as Quadrant IV perfume raw materials, perfume raw materials having a BP above about 250°C and a log P below about 3 are known as Quadrant II perfume raw materials, and perfume raw materials having a BP below about 250°C and a log P above about 3 are known as Quadrant III perfume raw materials.

[0068] Preferably, a perfume comprises a mixture of at least three, or even at least five, or at least seven perfume raw materials. A perfume may comprise at least 10, or at least 15 perfume raw materials. A mixture of perfume raw materials may provide more complex and desirable aesthetics and / or better perfume performance or longevity, for example, at various touchpoints. However, it may be desirable to limit the number of perfume raw materials in a perfume to reduce or limit formulation complexity and / or cost.

[0069] The fragrance may include at least one fragrance raw material of natural origin. Such ingredients may be desirable for sustainability / environmental reasons. The fragrance raw material of natural origin may include a natural extract or essence that may contain a mixture of PRMs. Such natural extracts or essences may include orange oil, lemon oil, rose extract, lavender, musk, patchouli, balsam essence, sandalwood oil, pine oil, cedar, etc.

[0070] The laundry detergent composition may include free perfume and / or encapsulated perfume.

[0071] The laundry detergent composition may comprise adjunct ingredients, which may be selected from hueing dyes, aesthetic dyes, builders preferably citric acid, chelating agents, cleaning polymers, dispersants, dye transfer inhibitor polymers, optical brighteners, opacifiers, defoamers, preservatives, antioxidants, or mixtures thereof. Preferably, the chelating agent is selected from aminocarboxylate chelating agents, aminophosphonate chelating agents, or mixtures thereof.

[0072] Preferably, the laundry detergent composition has a pH of from 6 to 10, more preferably from 6.5 to 8.9, most preferably from 7 to 8, the pH of the laundry detergent composition being measured as a 10% dilution in demineralised water at 20°C.

[0073] Liquid laundry detergent compositions may be Newtonian or non-Newtonian. Preferably, the liquid laundry detergent composition is non-Newtonian. Without wishing to be bound by theory, non-Newtonian liquids have different properties than Newtonian liquids. More specifically, the viscosity of non-Newtonian liquids depends on shear rate, while Newtonian liquids have a constant viscosity regardless of the applied shear rate. It is believed that the decrease in viscosity of non-Newtonian liquids upon the application of shear further facilitates dissolution of the liquid detergent. The liquid laundry detergent compositions described herein may have any suitable viscosity, depending on factors such as the formulated ingredients and the purpose of the composition. If Newtonian, the composition may have a viscosity value of 100 to 3,000 cP, alternatively 200 to 2,000 cP, or alternatively 300 to 1,000 cP, at a shear rate of 20 s-1 and a temperature of 20°C, according to the methods described herein. If non-Newtonian, the composition may have a high shear viscosity value of 100 to 3,000 cP, alternatively 300 to 2,000 cP, alternatively 500 to 1,000 cP, at a shear rate of 20 s-1 and a temperature of 20°C, and a low shear viscosity value of 500 to 100,000 cP, alternatively 1000 to 10,000 cP, alternatively 1,300 to 5,000 cP, at a shear rate of 1 s-1 and a temperature of 20°C, according to the methods described herein. Methods for measuring viscosity are known in the art. According to the present disclosure, viscosity measurements are performed using a rotational rheometer, such as a TA Instrument AR550. This instrument includes a 40 mm 2° or 1° cone fixture with a gap of approximately 50 to 60 μιη for isotonic liquids, or a 40 mm flat steel plate with a gap of 1000 μιη for liquid-containing particles. The measurements are performed using a flow procedure that includes a conditioning step, peak hold, and continuous ramp step. The conditioning step involves a 10-second preshear at a shear rate of 10 s, with the measurement temperature set at 20°C, followed by a 60-second equilibration at the selected temperature. The peak hold involves applying a shear rate of 0.05 s at 20°C for 3 minutes, with sampling every 10 seconds. The continuous ramp step is performed at shear rates from 0.1 to 1200 s at 20°C for 3 minutes to obtain the full flow profile.

[0074] Method for making water-soluble unit-dose articles Those skilled in the art will recognize known techniques and methods for making liquid laundry detergent compositions and water-soluble unit dose articles.

[0075] A method of making an article according to the present invention comprises the steps of: a) forming a film containing bacterial spores; and b) encapsulating the detergent composition in a water-soluble film.

[0076] A preferred method of incorporating bacterial spores into a water-soluble film involves creating a premix solution containing a water-soluble resin, bacterial spores, and other auxiliary film materials described herein, and then casting this solution to create an aqueous solution film with the bacterial spores dispersed therein.

[0077] How to use A further aspect of the present invention is a method for laundering fabrics, comprising the steps of: diluting a water-soluble unit dose article according to the present invention with water 200 to 3000 times, preferably 300 to 2000 times, to form a wash liquor; and contacting a fabric to be treated with the wash liquor.

[0078] The cleaning solution may contain water of any hardness, preferably varying from 0 gpg to 40 gpg.

[0079] Dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40 mm" is intended to mean "approximately 40 mm."

[0080] All documents cited herein, including any cross-referenced or related patents or patent applications, and any patent applications or patents to which this application claims priority or benefit, are incorporated herein by reference in their entirety, unless expressly excluded or otherwise limited. The citation of any document shall not be deemed to be prior art to any invention disclosed or claimed herein, or to teach, suggest, or disclose any such invention, either alone or in combination with any other reference or references. Furthermore, to the extent that any meaning or definition of a term in this document conflicts with any meaning or definition of the same term in a document incorporated by reference, the meaning or definition assigned to that term in this document shall control.

[0081] While particular embodiments of the present invention have been illustrated and described, it would be obvious to those skilled in the art that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention. [Example]

[0082] The following example compares the color stability of a soluble unit dose (SUD) detergent when Bacillus spores are added to the liquid detergent versus when they are added to a film used to encapsulate the liquid detergent. The results show that delivery of Bacillus spores to a film achieves a significant improvement in color stability compared to the same material added directly to the liquid detergent.

[0083] Detergents and stability testing methods The SUD liquid detergent was prepared with the following composition:

[0084] [Table 1] 1. Linear alkylbenzene sulfonates derived from α-olefins, which may be produced using the HF process, or may be produced using the AlCL3 (Friedel-Crafts) or DETAL process, or which contain more than 50% by weight of C12 α-olefins, or more than 75% by weight of C12 α-olefins, or which consist essentially of C12 α-olefins. 2. Natural oils such as C1214AE3S, which may be derived from coconut oil or palm kernel oil. 3. Lutensol FP620 - Ethoxylated Polyethyleneimine (PEI600 EO20) from BASF. 4. A polyethylene glycol graft polymer comprising a polyethylene glycol backbone (Pluriol E6000) and hydrophobic vinyl acetate side chains, the polyethylene glycol graft polymer comprising 40% by weight of the polymer system of the polyethylene glycol backbone polymer and 60% by weight of the polymer system of the grafted vinyl acetate side chains. 5. C12-18 fatty acids derived from palm kernel oil or which can be made from coconut oil. 6. Citric acid, which can be made synthetically or from natural sources. 7. Glycerin, which can be made from synthetic or natural sources. 8. Propylene glycol, which can be made from synthetic or natural sources.

[0085] Perfume composition:

[0086] [Table 2]

[0087] A polyvinyl alcohol film solution was prepared, with a final composition of 64 wt% Mowiol 18-88 solution (81365, Sigma-Aldrich), 20 wt% glycerol (Verbio Vereinigte BioEnergie AG, Leipzig), and 10 wt% D-sorbitol (S1876, Sigma-Aldrich). The solution was mixed at elevated temperature (100°C) until the PVA was completely dissolved. The solution was then mixed at room temperature until equilibrated. Bacillus spore premix (Genesis Biosciences, Cardiff, UK) was added to a 1.9 x 10 8 It was added to the PVA solution at a level to achieve a final activity of cfu / g dry PVA.

[0088] The solution was cast onto a plastic casting board overnight to obtain a PVA sheet of uniform thickness.

[0089] 22 g of SUD liquid detergent was weighed into three 60 mL clean glass jars. To the reference (A), 0.7 g of reference spore-free PVA film (MonoSol, Merrillville, IN, USA) was added. To a second jar (B), 0.7 g of Bacillus spore PVA film was added. Both were mixed to ensure contact between the detergent and the film. In a third jar (C), Bacillus spore powder (Genesis Biosciences, Cardiff, UK) was added to the liquid detergent to achieve spore activity equivalent to that delivered through the PVA film in jar B (1.33 x 10 8 cfu) and mixed thoroughly. 0.7 g of a reference spore-free PVA film was then added to Jar C and mixed to ensure contact between the detergent and the film.

[0090] [Table 3] The jar containing the detergent and film mixture was then placed in a 35°C oven.

[0091] After 3 weeks, 2 g of each detergent mixture stored at 35°C was pipetted into a 20 mL glass vial. * a * b * Measurements were taken using a DigiEye (VeriVide Ltd, Leicester, UK) at a shutter speed of 1 / 2 and a radius of 180°, which was calibrated before use by imaging in an open glass vial. Delta E values ​​were calculated by the ratio of the L of the reference (no spores) to the test plots using the following calculation: * a * b * Calculated from the values:

[0092]

number

[0093] [Table 4] * Bold letters are determined by Tukey-Kramer HSD, b * Significant differences in values ​​are indicated (p value ≦0.0001).

[0094] The SUD liquid detergent in Jar C containing Bacillus spores and PVA film showed a b * The test showed significant yellowing, measured as a significant increase in the Delta E value (more positive = more yellow), which is very noticeable to the eye, compared to a higher Delta E of 3.97. * However, this yellowing of the liquid detergent did not occur when the same amount of Bacillus spores was incorporated into the PVA film. The results suggest that Bacillus spores cause color stability issues in SUD liquid detergents, but that this can be mitigated by delivering the Bacillus spores into the film.

Claims

1. A water-soluble unit-dose detergent article comprising a water-soluble film and a detergent composition, wherein the water-soluble film comprises bacterial spores.

2. The article of claim 1 , wherein the bacterial spores comprise Bacillus spores.

3. 3. The article of claim 1 or 2, wherein the Bacillus spores comprise a bacterium selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus thuringiensis, Bacillus coagulans, and mixtures thereof.

4. The film is 1×10 ３ ~1 x 10 １１ The article of any one of claims 1 to 3, comprising CFU / g of bacterial spores.

5. The article according to any one of claims 1 to 4, wherein the film comprises a water-soluble resin comprising polyvinyl alcohol.

6. The article of any one of claims 1 to 4, wherein the film comprises a water-soluble resin comprising a material selected from the group consisting of proteins, polysaccharides, and mixtures thereof.

7. The article of claim 6 , wherein the film comprises a water-soluble resin comprising casein.

8. The article of claim 6 , wherein the film comprises a water-soluble resin that includes pectin.

9. The article of claim 6 , wherein the film comprises a water-soluble resin that includes carrageenan.

10. 10. The article of any one of claims 1 to 9, wherein the film further comprises one or more additives selected from the group consisting of plasticizers, plasticizer compatibilizers, lubricants, release agents, fillers, extenders, crosslinkers, antiblocking agents, antioxidants, detackifying agents, antifoaming agents, nanoparticles, bleaching agents, surfactants, bittering agents, and combinations thereof.

11. The article of any one of claims 1 to 10, wherein the film is coated with a powder.

12. The article of any one of claims 1 to 11, wherein the detergent composition comprises from 5% to 60% of a non-soap anionic surfactant, by weight of the detergent composition.

13. The article of any one of claims 1 to 12, wherein the detergent composition comprises from 2.5% to 30% nonionic surfactant, by weight of the detergent composition.

14. The article of any one of claims 1 to 13, wherein the detergent composition comprises from 1% to 15% water, by weight of the detergent composition.

15. 15. The article of any one of claims 1 to 14, wherein the detergent composition further comprises an alkanolamine selected from the group comprising monoethanolamine, diethanolamine, triethanolamine, and mixtures thereof, preferably monoethanolamine.

16. The article of any one of claims 1 to 15, wherein the detergent composition further comprises a perfume.

17. 17. The article of any one of claims 1 to 16, wherein the detergent composition comprises adjuvants comprising one or more of soap surfactants, peroxy compounds, bleach activators, anti-redeposition agents, neutralizing agents, optical brighteners, suds suppressors, chelating agents, builders, enzymes, color-adjusting dyes, bittering agents, dye transfer inhibitors, soil release agents, water softeners, electrolytes, pH adjusters, graying inhibitors, anti-wrinkle ingredients, bleaching agents, reducing agents, colorants, perfume capsules, processing aids, and mixtures thereof.

18. 18. A method of making the article of any one of claims 1 to 17, said method comprising the steps of: a) forming a film containing bacterial spores; and b) encapsulating the detergent composition in a water-soluble film.

19. A method of laundering fabrics, further comprising contacting the fabrics with an aqueous solution comprising the article of any one of claims 1 to 15.

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