Fiber protection agents, compositions and uses thereof

Abstract

Fiber protection agents, compositions and uses thereof The present disclosure relates to textile protection effects provided by odorant-binding proteins (OBPs) and / or modified odorant-binding proteins. The application of OBP proteins directly or in cosmetic compositions improves the heat protection and mechanical toughness of biological textiles, including hair, nails, skin, and other health care treatments, i.e., the resistance of hair fibers to heat treatments and mechanical stress. These proteins can be used in formulations / compositions for cosmetics, hair care compositions, nail, skin, and health care treatments.

Description

[Technical Field]

[0001] The present disclosure relates to textile protection effects provided by odorant-binding proteins (OBPs) and / or modified odorant-binding proteins. The application of OBP proteins directly or in cosmetic compositions improves the heat protection and mechanical toughness of biological textiles, including hair, nails, skin, and other health care treatments, i.e., the resistance of hair fibers to heat treatments and mechanical stress. These proteins can be used in formulations / compositions for cosmetics, hair care compositions, nail, skin, and health care treatments. [Background technology]

[0002] Odorant-binding proteins (OBPs) are small, water-soluble proteins that belong to the lipocalin superfamily. In nature, the function of these proteins is to transport hydrophobic odorant molecules across the aqueous mucus barrier to olfactory receptors, where a series of signals are sent to the brain.

[0003] Odorant-binding proteins from several mammals have been isolated and identified from the nasal mucus of, for example, cows, pigs, wild boars, giant pandas, mice, rats, and humans.

[0004] The DNA sequences of mammalian OBPs share low similarity. For example, the DNA sequence similarity between porcine OBP (OBP-I) and human OBP (hOBPIIa) is only 13.9%, while that between OBP-I and bovine OBP is 42.7%. Despite this low similarity, mammalian OBPs share several common characteristics, including a conserved tertiary structure: a β-barrel structure with eight β-strands connected by seven loops, a short α-helix near the C-terminus, and a ninth β-strand following a disordered C-terminal tail. The structure of OBPs is highly stable and resistant to degradation by temperature, organic solvents, pH fluctuations, or protease digestion. FT-IR spectra of porcine OBPs showed a stable structure up to 80°C, especially in the presence of ligands. Furthermore, vertebrate OBPs have the ability to reverse protein unfolding even after denaturation.

[0005] Filipa Goncalves et al. (2018) "Two engineered OBPs with opposite temperature-dependent affinities for 1-aminoanthracene" included two recombinant proteins based on the porcine OBP sequence: (i) a truncated OBP (tOBP-F44A / F66A) in which the first 16 residues at the N-terminus were deleted and two phenylalanine residues in the binding pocket were replaced with alanine residues (F44A and F66A), and (ii) OBP::GQ, in which OBP-I was fused to a spacer of 20 glycine-glutamine repeats and the anchor peptide SP-DS3. 20 Experimental and molecular modeling data show that the 1-AMA model ligand preferentially binds to tOBP-F44A / F66A at 25°C, whereas the ligand preferentially binds to OBP::GQ at 37°C. 20 ::SP-DS3 was shown to bind to the

[0006] Filipa Goncalves et al. (2018) "OBP fused to cell-penetrating peptides enhances delivery into liposomes" describes the fusion of porcine OBP with cell-penetrating peptides (CPPs, e.g., TAT, Pep-1, and pVEC). This study revealed that the delivery efficiency of l-AMA into liposomes varied.

[0007] Filipa Goncalves et al. (2018) "Incorporation of 1-aminoanthracene into liposomes is facilitated by the proximity of odorant-binding proteins" reported that the spacer (GQ 20 The design of two fusion proteins based on porcine OBP fused to the anchor peptide SP-DS3 with or without a spacer has been described. This study demonstrated that the proximity of the protein anchored to the liposome membrane (the advantage of the absence of a spacer) facilitates the incorporation of l-AMA into liposomes.

[0008] Filipa Goncalves et al. (2019) "Fragrance release from cotton functionalized with carbohydrate-binding module proteins" uses a spacer (GQ 20 The design of a fusion protein based on porcine OBP fused with a soluble cellulose (OBP) and a carbohydrate-binding module (CBM) has been described. This study demonstrated that the protein exhibited affinity for one fragrance (β-citronellol) and released this fragrance from cotton in the presence of sweat. However, the release ability of this fusion protein in the presence of sweat was inferior to that of native OBP.

[0009] Alessandro Capo et al. (2018) "Pig Odor Binding Protein as a Molecular Probe for Benzene Detection" discloses the use of porcine odorant binding proteins as probes in a fluorescent assay to detect benzene in the atmosphere.

[0010] Nunzio Cennamo et al. (2015) "A Simple Plastic Optical Fiber-Based Biosensor for Butanal Detection" discloses the detection of butanal (20-1000 μM) using a competitive assay with porcine odorant-binding proteins. This aldehyde is highly toxic and poses a high risk to human health, including cytotoxicity and carcinogenicity. The authors describe an optical biosensor for the detection of butanol in liquid samples.

[0011] Carla Silva et al. (2014) "Odorant Binding Proteins: Biotechnology Tools for Odor Control" discloses the use of porcine odorant binding proteins to release perfumes from cotton fabrics and mask the odor of smoke. The authors confirmed the functionalization of OBPs on fabrics. They only tested the release of one type of perfume from the fibers.

[0012] Paolo Pelosi et al. (2014) "Structures of odorant-binding proteins and their applications in biotechnology" disclose the potential use of OBPs as sensors for volatile substances and for the sustained release of odorant molecules.

[0013] Mariella Parisi et al. (2003) "Unfolding and refolding of porcine odorant binding protein in guanidine hydrochloride: equilibrium studies at neutral pH" discloses the denaturing effect of guanidine hydrochloride, a well-known chaotropic agent, on protein folding / unfolding. The aim of this fundamental study was to understand the structure of OBP protein as it unfolds and refolds.

[0014] Patent document WO0123890A1 discloses a detector array based on sensing elements in a solid support for use with the gaseous state of clinical samples or cell extracts. This is an immunoassay that utilizes viral peptides.

[0015] Patent document EP0335654A3 ​​discloses a method for isolating the gene for a rat-derived odorant-binding protein and a method for producing the protein.

[0016] Patent document WO2001012806A3 discloses that OBP-II can be used as a fixative for hydrophobic ligands such as odorants, which can be used in personal hygiene, agri-food systems, nutrition and therapeutic applications.

[0017] Patent document WO2022 / 003655A1 discloses the perfume release mechanism of mammalian odorant-binding proteins (OBPs) in the presence of sweat and uses of OBPs.

[0018] These facts are disclosed to explain the technical problem that the present disclosure is intended to solve. Summary of the Invention

[0019] The present disclosure relates to textile protection, i.e., textile thermal / heat protection, provided by odorant binding proteins (OBPs), synthetic / artificial odorant binding proteins, or mixtures thereof, preferably mammalian-derived odorant binding proteins.

[0020] The present disclosure also relates to odorant-binding proteins, preferably odorant-binding proteins (including synthetic / artificial OBPs), as heat protection agents for hair. These proteins protect hair from heat damage caused by heat treatment with high-temperature equipment, while maintaining the mechanical properties of hair and imparting good texture, such as softness and moisturization, to hair.

[0021] The present invention discloses novel applications and formulations that use odorant-binding proteins to enhance the heat protection and mechanical toughness of biological fibers, including hair, nails, skin, and other health care treatments. By incorporating odorant-binding proteins into cosmetic formulations, the resistance of these fibers to heat treatment and mechanical stress is significantly improved, providing excellent protection and strengthening effects against damage. The present disclosure includes methods for formulating compositions and applications in the fields of cosmetics, hair care, nail care, skin care, and health care.

[0022] The incorporation of odorant-binding proteins into cosmetic compositions provides multiple benefits, such as improved resistance to heat treatments and mechanical stress, prevention of fiber damage and / or breakage, and improvement of the overall health and appearance of biological fibers, such as hair fibers.

[0023] One aspect of the present disclosure relates to the use of cosmetic compositions comprising odorant-binding proteins in hair care and hair treatment, particularly as heat protection agents for hair.

[0024] Another aspect of the present disclosure relates to a composition for hair care and hair treatment, comprising an odorant-binding protein (a mammalian-derived odorant-binding protein, a synthetic odorant-binding protein, or an artificial odorant-binding protein).

[0025] Odorant-binding proteins (OBPs) are a type of protein that play an important role in the sense of smell in various organisms, including insects, mammals, and other animals. These proteins are primarily found in sensory organs, such as the antennae of insects and the olfactory epithelium of mammals. OBPs are responsible for binding and transporting odor molecules (odorants) from the surrounding environment to specific receptors on sensory neurons. By binding to these odorants, OBPs facilitate the recognition and transmission of olfactory signals, allowing organisms to perceive and distinguish between different scents.

[0026] Synthetic proteins are artificially designed or chemically synthesized protein molecules that do not occur naturally in living organisms. Scientists create these proteins by assembling amino acids in specific sequences using chemical synthesis techniques such as solid-phase peptide synthesis.

[0027] An artificial protein is a protein that does not occur in nature or that has been significantly designed or modified from a naturally occurring protein. This includes synthetic proteins, but also proteins made by genetic engineering techniques, e.g., recombinant proteins.

[0028] Another aspect of the present disclosure relates to a composition for hair care and hair treatment comprising: 0.005% to 1% of an isolated or artificial protein having at least 90%, preferably at least 95%, identity to an amino acid sequence selected from the list of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, or a mixture thereof.

[0029] Sequence alignment techniques for sequence comparison are well known to those skilled in the art and include methods such as GAP, BESTFIT, BLAST, FASTA, and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48:443-453) to perform a global (entire sequence) alignment of two sequences, maximizing the number of matches and minimizing the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215:403-10) calculates the percent identity between sequences and performs a statistical analysis of the similarity between two sequences. Software for BLAST analysis is available through the National Center for Biotechnology Information (NCBI). Global similarity and identity percentages can also be determined using any of the techniques available in the MatGAT software package (Campanella et al., BMC Bioinformatics. 2003 Jul 10;4:29. MatGAT: A similarity / identity matrix generation application using protein or DNA sequences). Minor manual editing, apparent to those skilled in the art, can be performed to optimize alignment between conserved motifs. The sequence identity values ​​presented herein were determined as a percentage across the entire amino acid sequence using the default parameters of BLAST.

[0030] In one embodiment, the heat protection properties of odorant-binding proteins (mammalian-derived odorant-binding proteins, synthetic odorant-binding proteins, and artificial odorant-binding proteins) were confirmed by applying the proteins to hair and then treating the hair with a high-temperature device such as a flat iron. The heat resistance of the treated hair was evaluated by measuring the Young's modulus parameters using a texture analyzer (Figure 1).

[0031] In one embodiment, the composition is applied to dry or wet hair at a concentration ranging from 0.005% to 1% (w / v). Heat treatment with a flat iron is performed multiple times on each tress, up to 250°C.

[0032] In one embodiment, the composition also improves the mechanical resistance of the hair (Figure 2).

[0033] In one embodiment, the odorant binding protein (mammalian-derived odorant binding protein, synthetic odorant binding protein, artificial odorant binding protein) is stable in polar and non-polar solvents such as methanol, butanol, benzene, propylene glycol, undecanol, and buffer solutions (Tris-HCl, phosphate, PBS).

[0034] Furthermore, in one embodiment, the odorant-binding protein (mammalian-derived odorant-binding protein, synthetic odorant-binding protein, artificial odorant-binding protein) is stable in a temperature range of 18°C ​​to 70°C, preferably 18°C ​​to 60°C, and a pH range of 3.0 to 10.0.

[0035] One aspect of the present disclosure relates to the incorporation of odorant-binding proteins (mammalian-derived odorant-binding proteins, synthetic odorant-binding proteins, artificial odorant-binding proteins) into cosmetic compositions, such as hair care or hair treatment formulations.

[0036] In one embodiment, the native form of the mammalian odorant-binding protein is obtained from pig, human, dog, cat, rat, mouse, cow, wild boar, panda, Chinese hamster, Meishan pig, guinea pig, Tibetan pig, horse, dolphin or chimpanzee.

[0037] In one embodiment, cosmetic applications comprising the compositions of the present disclosure are used in hair care and hair treatment.

[0038] Further, in one embodiment, the composition for hair care applications is in the form of a shampoo, a dry shampoo, a heat protectant for hair, a hair serum, a hair mask, a hair conditioner, or a coloring cream for hair.

[0039] One aspect of the present disclosure relates to a hair care kit comprising the composition of the present disclosure.

[0040] The use of odorant-binding proteins in cosmetic compositions to enhance the heat protection and mechanical toughness of biological fibers is an important advancement in the fields of cosmetics, hair care, nail care, skin care, and health care treatment. The present disclosure also relates to compositions that effectively protect and strengthen biological fibers, particularly hair fibers, from harmful environmental factors, improving their lifespan and quality.

[0041] One aspect of the present disclosure relates to a cosmetic composition for strengthening biological fabrics, comprising at least one fabric protectant and a suitable dermatological excipient and / or carrier, wherein the fabric protectant is a mammalian-derived odorant-binding protein, or a synthetic / artificial odorant-binding protein, or a mixture thereof.

[0042] In some embodiments, the fiber protectant can be used in a hair care composition, a skin care composition, a nail care composition, a cosmetic composition, or a pharmaceutical composition, preferably a hair care composition.

[0043] In one embodiment for obtaining better results, the odorant-binding protein includes at least one protein having a sequence length of 150 to 400 amino acids.

[0044] In one embodiment for obtaining better results, the odorant-binding protein comprises at least one protein having a sequence length of about 157 to 350 amino acids, preferably a protein having a sequence length of about 160 to 350 amino acids.

[0045] In one embodiment for obtaining better results, the odorant-binding protein comprises at least one protein having a sequence length of 157 to 350 amino acids, preferably a protein having a sequence length of 160 to 350 amino acids.

[0046] In one embodiment for better results, the OBP sequence is at least 90% identical, preferably at least 95% identical, or 100% identical to at least one amino acid sequence selected from the following list: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, or a mixture thereof. More preferably, it is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% identical to at least one of the following sequences: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22.

[0047] In one embodiment for better results, the OBP sequence is identical to at least one amino acid sequence selected from the following list: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, or a mixture thereof.

[0048] In one embodiment for better results, the odorant-binding protein is at least 90%, preferably at least 95% identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof. More preferably, it is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% identical to at least one of the following sequences: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

[0049] In one embodiment, for better results, the suitable dermatological excipients and / or carriers are suitable hair treatment excipients and / or carriers.

[0050] In one embodiment for better results, the composition is a hair care composition, a nail care composition, a skin care composition or a health care composition.

[0051] In one embodiment for obtaining better results, the composition is a composition for protecting the hair, ie for thermal protection or heat protection of the hair.

[0052] In one embodiment for better results, the composition is a hair care composition.

[0053] In one embodiment for obtaining better results, the amount of fiber protective agent is 0.005 to 1% (w / v), preferably 0.01 to 0.5% (w / v), more preferably 0.012% (w / v).

[0054] In one embodiment for better results, the suitable excipient is selected from the list consisting of a hair styling polymer, an emulsifier, a non-ionic base, or a mixture thereof.

[0055] In one embodiment for better results, the composition is in the form of a spray, serum or foam.

[0056] In one embodiment for better results, the composition is a shampoo, a dry shampoo, a hair serum, a hair mask, a hair conditioner, or a hair dye.

[0057] In one embodiment for better results, the composition further comprises an alcohol, an absorbent, a conditioner or a mixture thereof.

[0058] In one embodiment for better results, the composition comprises a solvent, preferably said solvent is selected from the list consisting of alcohol, benzene or mixtures thereof.

[0059] In one embodiment for better results, the alcohol is ethanol, methanol, butanol, undecanol, propylene glycol or mixtures thereof.

[0060] In one embodiment for better results, the composition further comprises at least one excipient selected from the following group: anionic surfactants, amphoteric surfactants, cationic surfactants, nonionic surfactants, preservatives, thickeners, naturally derived polymers, humectants, silicones, organic oils, proteins, fragrances, vitamins, emollient esters, alkanolamides, amines, buffers, pH adjusters, salts, fatty alcohols, UV filters, amine oxides, chelating agents, fatty acids, antibacterial agents, antimicrobial agents, PEG materials, polymers, antistatic agents, and alcohols.

[0061] In one embodiment for better results, the composition further comprises at least one compound selected from the group consisting of preservatives, thickeners, naturally derived polymers, humectants, silicones, organic oils, proteins, fragrances, vitamins, emollient esters, alkanolamides, amines, buffers, pH adjusters, salts, fatty alcohols, UV filters, amine oxides, chelating agents, fatty acids, antimicrobial agents, antibacterial agents, PEG materials, polymers, antistatic agents, alcohols, or mixtures thereof.

[0062] In one embodiment for better results, the at least one excipient suitable for dermatological applications is selected from ethanol, benzyl alcohol, diol molecules or mixtures thereof.

[0063] In one embodiment for better results, the diol molecule is selected from ethylene glycol, propylene glycol, butylene glycol, butylene glycol or mixtures thereof.

[0064] In one embodiment for better results, the composition may include: 0-30% (w / v) ethanol, preferably 10-20% (w / v); 0-3% (w / v) benzyl alcohol, preferably 0.5-2% (w / v); 0-20% (w / v) of diol molecules, preferably 10-15% (w / v).

[0065] In one embodiment for better results, the composition may further comprise a propellant, a fragrance, an oil or mixtures thereof.

[0066] Another aspect of the present disclosure relates to a thermal / heat protection agent for cosmetics for protecting biological fabrics, comprising a mammalian-derived odorant-binding protein, or a synthetic / artificial odorant-binding protein, or a mixture thereof.

[0067] In one embodiment for better results, the biological fabric protectant is a thermal or heat fabric protectant, preferably a thermal or heat hair protectant.

[0068] In one embodiment for obtaining better results, the odorant-binding protein includes at least one protein having a sequence length of 150 to 400 amino acids.

[0069] In one embodiment for obtaining better results, the odorant-binding protein includes at least one protein having a sequence length of 160 to 350 amino acids.

[0070] In one embodiment for better results, the sequence is at least 90%, preferably at least 95% identical to at least one amino acid sequence selected from the following list: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, or a mixture thereof.

[0071] In one embodiment for better results, the odorant-binding protein is at least 90%, preferably at least 95% identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

[0072] In one embodiment for better results, the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof. More preferably, it is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% identical to at least one of the following sequences: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22.

[0073] In one embodiment for better results, the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

[0074] Another aspect of the present disclosure relates to shampoos, lotions, serums, creams, conditioners, foams, elixirs, oils, aerosols, or masks comprising the compositions or thermal / heat protection agents described in this disclosure.

[0075] Another aspect of the present disclosure relates to a kit comprising the thermal / heat protection agent and / or cosmetic composition described in this disclosure.

[0076] Another aspect of the present disclosure relates to the use of an odorant-binding protein as a thermal / heat protection agent for biological fibers, wherein the odorant-binding protein is at least 90%, preferably at least 95%, identical to a sequence selected from the following list: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, or a mixture thereof. More preferably, it is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% identical to at least one of the following sequences: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22.

[0077] In one embodiment for better results, the fibers are hair fibers, nail fibers or skin fibers. Another aspect of the present disclosure relates to a method for increasing the heat protection and mechanical toughness of biological fibers, comprising applying to said fibers a composition described herein or a cosmetic protectant described herein. [Brief explanation of the drawings]

[0078] The following figures show preferred embodiments for illustrating the present disclosure and are not intended to limit the scope of the invention.

[0079] [Figure 1]Figure 1 shows the heat protection effect on hair imparted by application of the composition of the present disclosure. Young's modulus parameters obtained with a texture analyzer are shown for virgin hair treated with an OBP followed by a flat iron (5 seconds x 3 times). Statistical comparisons were performed using one-way ANOVA and post-hoc Turkey's test to identify significant differences between results. A p-value of <0.001 (****) was considered statistically significant. Bars indicate standard error. [Figure 2] Figure 2 shows the mechanical resistance effect of hair imparted by application of a composition of the present disclosure. Young's modulus parameters obtained with a texture analyzer are shown for bleached hair treated with OBP. Statistical comparisons were performed using one-way ANOVA and post-hoc Turkey's test to identify significant differences between results. A p-value of <0.001 (****) was considered statistically significant. Bars indicate standard error. DETAILED DESCRIPTION OF THE INVENTION

[0080] The present disclosure relates to the heat protection effect of textiles imparted by odorant-binding proteins (mammalian-derived odorant-binding proteins, synthetic odorant-binding proteins, artificial odorant-binding proteins) and / or modified odorant-binding proteins. The application of OBP proteins directly or in cosmetic compositions improves the heat protection and mechanical toughness of biological textiles, including hair, nails, skin, and other health care treatments, i.e., the resistance of hair fibers to heat treatment and mechanical stress. These proteins can be used in formulations / compositions for cosmetics, hair care compositions, nail, skin, and health care treatments.

[0081] The present disclosure also relates to a heat protection effect for hair imparted by applying a composition containing an odorant-binding protein (a mammalian-derived odorant-binding protein, a synthetic odorant-binding protein, or an artificial odorant-binding protein) to hair.

[0082] The present disclosure relates to the heat protection effect imparted to fabric care compositions, i.e., hair care protection, by odorant-binding proteins (OBPs). The application of OBP proteins improves the resistance of hair fibers to heat treatment and mechanical stress. These proteins can be used in the formulation of cosmetic and hair care compositions.

[0083] Another aspect of the present disclosure relates to compositions containing odorant-binding proteins for use in hair care and hair treatment.

[0084] One aspect of the present disclosure relates to the use of a cosmetic composition containing an odorant-binding protein for use in hair care and hair treatment, particularly as a heat protection agent for hair.

[0085] In one embodiment, improved resistance of hair to heat used in cosmetic treatments, such as for curling and straightening, has been observed.

[0086] In one embodiment, applying a composition containing OBP to hair also improved the mechanical resistance of damaged hair.

[0087] The present disclosure is particularly useful in the cosmetic field, especially in protecting fibers such as hair from heat used in cosmetic treatments such as curling and straightening / smoothing, especially in thermal hair care products.

[0088] In one embodiment, the resulting protein is stable in solutions prepared in different solvents (hydrogen peroxide, methanol, butanol, benzene, propylene glycol, undecanol, etc.), at different temperatures (18-60°C), and at different pH ranges (4.0-10.0).

[0089] In yet another embodiment, applying a composition containing a mammalian-derived odorant-binding protein to hair improves the thermal and mechanical resistance of the hair (see Figures 1 and 2).

[0090] Repeated hair straightening with high-temperature devices (e.g., flat irons) can damage hair, which has a significant impact on the health of the hair fiber. Applying OBP to hair provides increased protection against the damaging effects of heat.

[0091] In one embodiment, various active ingredients may also be added to the composition, including thermal protection formulas, mechanical resistance, styling primers, or mixtures thereof, allowing for a wide range of cosmetic applications.

[0092] In one embodiment, application of mammalian-derived OBP to virgin hair followed by flat ironing improves the heat protection of the hair (see Figure 1).

[0093] Figure 1 shows the heat protection of hair imparted by the application of a composition containing OBP. Young's modulus parameters were obtained using a texture analyzer when virgin hair was treated with OBP followed by a flat iron (5 seconds x 3 times). Statistical comparisons were performed by one-way analysis of variance (ANOVA) with post-hoc Turkey's test to identify significant differences in results. p-values ​​<0.001 ( **** ) was considered statistically significant. Bars indicate standard errors.

[0094] In one embodiment, application of a composition containing OBP to bleached (damaged) hair improves the thermal and mechanical resistance of the hair compared to bleached hair to which the composition is not applied (see Figure 2). OBP forms a protective barrier on the hair fiber, helping to restore some of the resistance properties of damaged hair.

[0095] Figure 2 shows the mechanical resistance of hair. Young's modulus parameters of bleached hair treated with OBP were obtained using a texture analyzer. Statistical comparisons were performed by one-way analysis of variance (ANOVA) with post-hoc Turkey's test to identify significant differences in the results. p-values ​​<0.001 ( ****) was considered statistically significant. Bars indicate standard errors.

[0096] Protein sequence list Protein sequences are written using the one-letter code for amino acids, as follows: [Table 1]

[0097] In one embodiment, the sequence is porcine OBP (PDB ID 1DZK) - SEQ ID NO:1. JPEG2026500830000002.jpg20166

[0098] In one embodiment the sequence is human OBPIIa (UniProt ID Q9NY56) - SEQ ID NO:2. JPEG2026500830000003.jpg20166

[0099] In one embodiment, the sequence is human OBPIIb (UniProt ID Q9NPH6) - SEQ ID NO:3. JPEG2026500830000004.jpg21166

[0100] In one embodiment the sequence is mouse OBP (UniProt ID OBP1A) - SEQ ID NO:4. JPEG2026500830000005.jpg21166

[0101] In one embodiment, the sequence is mouse OBP2A (UniProt ID Q8K1H9) - SEQ ID NO:5. JPEG2026500830000006.jpg21166

[0102] In one embodiment, the sequence is mouse OBP1B (UniProt ID A2AEP0) - SEQ ID NO:6. JPEG2026500830000007.jpg22166

[0103] In one embodiment, the sequence is rat OBP (PDB ID 3FIQ) - SEQ ID NO:7. JPEG2026500830000008.jpg21166

[0104] In one embodiment, the sequence is rat OBP (PDB ID 3ZQ3) - SEQ ID NO:8. JPEG2026500830000009.jpg22166

[0105] In one embodiment the sequence is rat OBP (UniProt ID P08937) - SEQ ID NO:9. JPEG2026500830000010.jpg21166

[0106] In one embodiment, the sequence is bovine OBP (PDB ID 1OBP) - SEQ ID NO:10. JPEG2026500830000011.jpg21166

[0107] In one embodiment the sequence is Sus scrofa OBP (PDB ID 1GM6) - SEQ ID NO:11. JPEG2026500830000012.jpg22166

[0108] In one embodiment, the sequence is panda OBP (PDB ID 5NGH) - SEQ ID NO:12. JPEG2026500830000013.jpg21166

[0109] In one embodiment, the sequence is Chinese hamster OBP (Ensembl ID ENSCGRP00015014591.1) - SEQ ID NO:13. JPEG2026500830000014.jpg21166

[0110] In one embodiment, the sequence is Meishan pig OBP (Ensembl ID ENSSSCP00040041163.1) - SEQ ID NO:14. JPEG2026500830000015.jpg19166

[0111] In one embodiment the sequence is equine OBP (Ensembl ID ENSECAP00000000103.2) - SEQ ID NO:15. JPEG2026500830000016.jpg20166

[0112] In one embodiment the sequence is guinea pig OBP (Ensembl ID ENSCPOP00000016393.2) - SEQ ID NO:16. JPEG2026500830000017.jpg21166

[0113] In one embodiment the sequence is canine OBP (Ensembl ID ENSCAFP00040020992.1) - SEQ ID NO:17. JPEG2026500830000018.jpg20166

[0114] In one embodiment the sequence is Tibetan Pig OBP (Ensembl ID ENSSSCP00015013912.1) - SEQ ID NO:18. JPEG2026500830000019.jpg21166

[0115] In one embodiment the sequence is Domestic Cat OBP (Ensembl ID ENSFCAP00000053707.1) - SEQ ID NO:19. JPEG2026500830000020.jpg31166

[0116] In one embodiment, the sequence is chimpanzee OBP (Ensembl ID ENSPTRP00000048681.3) - SEQ ID NO:20. JPEG2026500830000021.jpg20166

[0117] In one embodiment, the sequence is OBP::GQ20::KP (recombinant protein)-SEQ ID NO:21. JPEG2026500830000022.jpg26166

[0118] In one embodiment, the sequence is OBP::GQ20::CBM (recombinant protein)-SEQ ID NO:22. JPEG2026500830000023.jpg35166 [Example]

[0119] As an example, proteins SEQ ID NO: 1 and SEQ ID NO: 21 were each applied to Asian virgin hair for 5 minutes. After drying, a flat iron was applied to the hair three times for 5 seconds each. The mechanical properties of 30 hairs were analyzed using a texture analyzer before and after application of the heat treatment device.

[0120] As an example, protein SEQ ID NO: 21 was applied to Asian virgin hair for 10 and 60 minutes. Thermal testing of the hair was performed using a power-compensated differential scanning calorimeter (DSC) at temperatures ranging from 50°C to 265°C (heating rate: 5°C / min) (Table 1). The increase in heat / energy (Delta H) required to destroy hair was measured with and without the protein present on the hair. These results indicated that OBP protein has a protective effect on hair against thermal damage.

[0121] [Table 2] [Example]

[0122] As an example, protein SEQ ID NO:21 was formulated into a dry shampoo composition containing the following components: propellant, solvent, emulsifier, preservative, surfactant / cleaning agent, fragrance, fragrance, antibacterial / antibacterial agent, viscosity modifier, and absorbent / mattifying agent. When applied to human hair of a panel of 15 volunteers, the composition demonstrated a high level of effectiveness in eliminating human sweat odor. Over 85.7% of the panel detected no sweat odor after exercise, and 60% of the panel detected a brief or moderate odor (added to the protein in the dry shampoo). Using technical analysis (GC-MS), over 48.6% of the perfume bound to the protein was released in the presence of sweat from the hair within the first hour, and over 74.6% of the perfume was released after one hour.

[0123] As used herein, the terms "determining," "measuring," "evaluating," "assessing," "assaying," and "analyzing" are often used interchangeably and refer to forms of measurement. These terms include determining whether an element is present or not (e.g., detecting). These terms can include quantitative, qualitative, or quantitative and qualitative determinations. Evaluation can be relative or absolute. "Detecting the presence" can include determining the amount present, as well as determining presence or absence, depending on the context.

[0124] As used herein, the terms "pharmaceutically acceptable" and "cosmetically acceptable" are used interchangeably and refer to compounds, materials, compositions, and / or dosage forms that are medically suitable for use in contact with human and animal tissues without undue toxicity, irritation, allergic reaction, or other problems or complications, and at a reasonable benefit / risk ratio. More specifically, pharmaceutically / cosmetically acceptable refers to materials, compounds, or compositions that are suitable for use in contact with skin, scalp, or hair. Pharmaceutically / cosmetically acceptable materials are known to those skilled in the art.

[0125] As used herein, the terms "treatment" or "treating" refer to a pharmaceutical or other intervention regimen to obtain a beneficial or desired result in a recipient. Beneficial or desired results include, but are not limited to, a therapeutic effect and / or a prophylactic effect. A therapeutic effect may refer to the eradication or alleviation of the symptom or underlying disease being treated. A therapeutic effect may also be achieved when a subject experiences improvement due to the eradication or alleviation of one or more physiological symptoms associated with the underlying disease, although the subject may still suffer from the underlying disease. A prophylactic effect includes delaying, preventing, or eliminating the onset of a disease or condition, delaying or eliminating the onset of symptoms of a disease or condition, slowing, halting, or reversing the progression of a disease or condition, or any combination thereof. For prophylactic benefit, treatment can be administered to subjects at risk of developing a particular disease or who report one or more physiological symptoms of a disease, but who may not have been diagnosed with the disease.

[0126] Where the singular form of an element or feature is used in a claim, the plural form is also included, and vice versa, unless specifically excluded. For example, the terms "a peptide" or "the peptide" include the plural forms "peptides" or "the peptides," and vice versa. In the claims, articles such as "a," "an," and "the" can mean one or more unless specifically indicated to the contrary or clear from the context. A claim or description containing "or" between one or more group members is satisfied if one, more than one, or all of the group members are present in, used in, or associated with the product or process, unless specifically indicated to the contrary or clear from the context. The disclosure includes embodiments in which exactly one member of a group is present in, used in, or associated with the product or process. It also includes embodiments in which more than one or all members of a group are present in, used in, or associated with the product or process.

[0127] Furthermore, the present invention encompasses all variations, combinations, and permutations in which one or more limitations, elements, clauses, descriptive terms, etc. from one or more claims or relevant portions of the specification are introduced into other claims. For example, a claim that is dependent on another claim can be amended to include one or more limitations set forth in the other claims that are dependent on the same base claim.

[0128] Furthermore, when a claim recites a composition, it is understood that the claim also includes methods of using the composition for any purpose disclosed herein, and also includes methods of making the composition by any of the methods disclosed herein or other methods known to those of skill in the art, unless specifically indicated otherwise or where a contradiction or inconsistency would arise.

[0129] When ranges are expressed, they are intended to include the endpoints. Furthermore, unless specifically stated otherwise or apparent from the context and / or the understanding of one of ordinary skill in the art, unless the context clearly dictates otherwise, values ​​expressed as ranges are understood to be able to take any particular value, in different embodiments of the invention, to an accuracy of 1 / 10 of the unit of the lower limit of the range. Unless specifically stated otherwise or apparent from the context and / or the understanding of one of ordinary skill in the art, values ​​expressed as ranges are also understood to be able to take any subrange within the given range, with the endpoints of that subrange expressed to an accuracy of 1 / 10 of the unit of the lower limit of the range.

[0130] Where the term "comprising" is used herein, it is intended to indicate the presence of stated features, components, steps or ingredients, but does not exclude the presence or addition of one or more other features, components, steps or ingredients or groups thereof.

[0131] The present disclosure is not in any way limited to the described embodiments, as those skilled in the art can envision many possible modifications.

[0132] The above embodiments are described as being combinable.

Claims

1. A cosmetic composition for strengthening biological fibers, comprising an effective amount of at least one fiber protection agent and a suitable dermatological excipient and / or carrier, wherein the fiber protection agent is a mammalian-derived odorant-binding protein, or a synthetic / artificial odorant-binding protein, or a mixture thereof.

2. The cosmetic composition according to any one of the preceding claims, wherein the odorant-binding protein comprises at least one protein having a sequence length of 150 to 400 amino acids.

3. The cosmetic composition according to the preceding claim, wherein the odorant-binding protein comprises at least one protein having a sequence length of about 157 to 350 amino acids, preferably a protein having a sequence length of about 160 to 350 amino acids.

4. The cosmetic composition according to claim 1 , wherein the odorant-binding protein comprises at least one protein having a sequence length of 157 to 350 amino acids.

5. A cosmetic composition according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is at least 90% identical to at least one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

6. A cosmetic composition according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is at least 95% identical to at least one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

7. A cosmetic composition according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is at least identical to at least one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

8. A cosmetic composition according to any one of the preceding claims, wherein the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

9. 10. A cosmetic composition according to any one of the preceding claims, wherein the suitable dermatological excipients and / or carriers are suitable hair treatment excipients and / or carriers.

10. The cosmetic composition according to any one of the preceding claims, wherein the composition is a hair care composition, a nail care composition, a skin care composition, or a health care composition.

11. 10. A cosmetic composition according to any one of the preceding claims, wherein the composition is a composition for protecting hair, i.e. for thermal or heat protection of hair.

12. The cosmetic composition according to any one of the preceding claims, wherein the composition is a hair care composition.

13. 10. The cosmetic composition according to the preceding claim, wherein the amount of the fiber protective agent is 0.005 to 1% (w / v), preferably 0.01 to 0.5% (w / v), more preferably 0.012 to 0.1% (w / v).

14. 10. The cosmetic composition according to any one of the preceding claims, wherein the suitable excipient is selected from the list consisting of a hair styling polymer, an emulsifier, a non-ionic base, or a mixture thereof.

15. 10. The cosmetic composition according to any one of the preceding claims, wherein the composition is in the form of a spray, serum or foam.

16. 10. The cosmetic composition according to any one of the preceding claims, wherein the composition is a shampoo, a dry shampoo, a hair serum, a hair mask, a hair conditioner, or a hair dye.

17. The cosmetic composition according to any one of the preceding claims, wherein the composition further comprises an alcohol, an absorbent, a conditioner, or a mixture thereof.

18. 10. The cosmetic composition according to claim 9, wherein the composition comprises a solvent, preferably the solvent is selected from the list consisting of alcohol, benzene, or mixtures thereof.

19. 10. The cosmetic composition according to claim 1, wherein the alcohol is ethanol, methanol, butanol, undecanol, propylene glycol, or a mixture thereof.

20. 10. The cosmetic composition according to any one of the preceding claims, further comprising at least one excipient selected from the following group: anionic surfactants, amphoteric surfactants, cationic surfactants, nonionic surfactants, preservatives, thickeners, naturally derived polymers, moisturizers, silicones, organic oils, proteins, fragrances, vitamins, emollient esters, alkanolamides, amines, buffers, pH adjusters, salts, fatty alcohols, UV filters, amine oxides, chelating agents, fatty acids, antibacterial agents, antimicrobial agents, PEG materials, polymers, antistatic agents, and alcohols.

21. 10. The cosmetic composition of claim 1, further comprising at least one compound selected from the group consisting of preservatives, thickeners, naturally occurring polymers, moisturizers, silicones, organic oils, proteins, fragrances, vitamins, emollient esters, alkanolamides, amines, buffers, pH adjusters, salts, fatty alcohols, UV filters, amine oxides, chelating agents, fatty acids, antimicrobial agents, antibacterial agents, PEG materials, polymers, antistatic agents, alcohols, or mixtures thereof.

22. 10. The cosmetic composition according to claim 1, wherein the at least one excipient suitable for dermatological use is selected from ethanol, benzyl alcohol, diol molecules, or mixtures thereof.

23. 10. The cosmetic composition according to claim 1, wherein the diol molecule is selected from ethylene glycol, propylene glycol, butylene glycol, butylene glycol, or mixtures thereof.

24. 10. The cosmetic composition according to any one of the preceding claims, comprising: 0-30% (w / v), preferably 10-20% (w / v) ethanol; 0-3% (w / v), preferably 0.5-2% (w / v) benzyl alcohol; 0-20% (w / v), preferably 10-15% (w / v) of diol molecules.

25. 10. The composition of any one of the preceding claims, further comprising a propellant, a fragrance, an oil, or a mixture thereof.

26. A thermal / heat protection agent for cosmetics for protecting biological fabrics, comprising a mammalian-derived odorant-binding protein, or a synthetic / artificial odorant-binding protein, or a mixture thereof.

27. 10. The agent according to the preceding claim, wherein the biological textile protection agent is a textile thermal or heat protection agent, preferably a hair thermal or heat protection agent.

28. The agent according to any one of the preceding claims, wherein the odorant-binding protein comprises at least one protein having a sequence length of 150 to 400 amino acids.

29. The agent according to claim 1 , wherein the odorant-binding protein comprises at least one protein having a sequence length of 160 to 350 amino acids.

30. The agent according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is at least 90% identical to at least one sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

31. The agent according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is at least 95% identical to one sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

32. The agent according to any one of the preceding claims, wherein the sequence of the odorant-binding protein is identical to one sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

33. The agent according to any one of the preceding claims, wherein the odorant-binding protein is one sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

34. The agent according to any one of the preceding claims, wherein the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

35. The agent according to any one of the preceding claims, wherein the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

36. A shampoo, lotion, serum, cream, conditioner, foam, elixir, oil, aerosol or mask comprising a composition and / or a thermal / heat protection agent according to any one of the preceding claims.

37. A kit comprising the thermal protection agent and / or the cosmetic composition according to any one of the preceding claims.

38. Use of an odorant-binding protein as a thermal / heat protection agent for biological fibers, wherein the odorant-binding protein is a mammalian-derived odorant-binding protein, or a synthetic / artificial odorant-binding protein, or a mixture thereof.

39. Use of a composition according to any one of the preceding claims, wherein the odorant-binding protein is at least 90% identical to at least one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

40. Use of a composition according to any one of the preceding claims, wherein the odorant-binding protein is at least 95% identical to at least one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

41. Use of a composition according to any one of the preceding claims, wherein the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

42. Use of a composition described in any one of the preceding claims, wherein the odorant-binding protein is identical to one amino acid sequence selected from the following list: SEQ ID NO: 1, SEQ ID NO: 21, SEQ ID NO: 22, or a mixture thereof.

43. 10. Use of a composition according to any one of the preceding claims, wherein the fibres are hair fibres, nail fibres or skin fibres.

44. A method for increasing the heat protection and mechanical toughness of biological fibers, comprising applying to said fibers a composition according to any one of the preceding claims or a cosmetic agent according to any one of the preceding claims.

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