How to determine skin condition by UPE measurement

By acclimatizing in a darkroom to measure UPE across the face, the method addresses the challenge of wide-area UPE measurement, enabling precise skin condition assessment and tailored skincare solutions.

JP7597706B2Active Publication Date: 2024-12-10SHISEIDO CO LTD
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Patent Information

Application Number
JP2021513725
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2019-04-12
Filing Date
2020-04-10
Publication Date
2024-12-10
Estimated Expiration
2040-04-10

AI Technical Summary

Technical Problem

Existing methods struggle to measure ultra-weak photon emission (UPE) over a wide area such as the entire face due to its very weak luminescence, and the influence of external stimuli like ultraviolet rays has not been adequately addressed.

Method used

A method involving darkroom acclimatization to eliminate external stimuli, allowing for UPE measurement across the entire face using UPE imaging or a photo counter, followed by determining skin conditions based on UPE intensity variations.

Benefits of technology

Enables accurate determination of skin conditions, including oxidation levels and potential future skin problems, by measuring UPE intensity across the face, and providing personalized skin care recommendations.

✦ Generated by Eureka AI based on patent content.

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Abstract

[Problem] To provide a method that measures UPE, which is a luminous phenomenon that accompanies a biochemical reaction in organisms, and determines the condition of skin on the basis of the measured UPE intensity. [Solution] The condition of skin can be determined by removing influences from external stimuli by means of an acclimation process in a dark room, and detecting only UPE generated as a result of a biochemical reaction naturally occurring in the skin.
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Description

[Technical field]

[0001] The present invention relates to a technique for measuring UPE occurring over the entire face, and to a method for determining the skin condition of each part of the face by measuring UPE in each part of the face. [Background technology]

[0002] UPE (Ultra-weak photon emission) is a weak light emitted from living organisms, a luminescence phenomenon that occurs during biochemical reactions in the body. It is light emitted during biochemical reactions, including energy metabolism, that mainly involve reactive oxygen, and its mechanisms are diverse. UPE is also called biophotons, biophotons, extremely weak bioluminescence, and extremely weak biochemiluminescence.

[0003] That is, luminescence by UPE is mainly generated by the energy produced by redox reactions in biochemical reactions. In particular, luminescence by UPE occurs in biochemical reactions accompanied by the production of reactive oxygen species and free radicals, and the actual luminescence is due to the excitation of molecules produced during the oxidative modification process of various substances that compose living organisms.

[0004] The production of reactive oxygen species in the body is closely related to metabolic processes in the body and oxidative stress caused by external factors such as ultraviolet rays, radiation, drugs, and environmental pollutants. In addition, an increase in UPE due to the production of reactive oxygen species in the body is related to stress such as oxidative damage. For example, when oxidation of the skin progresses due to exposure to electromagnetic waves such as ultraviolet rays, the amount of UPE released increases. In particular, UPE that increases due to external stimuli is called delayed luminescence.

[0005] As part of research into the effects of ultraviolet (UV) rays on the skin, the results of a study measuring the transmittance of UV rays through the stratum corneum and epidermis have been reported (Non-Patent Document 1).

[0006] On the other hand, as a technology for measuring UPE, a method using an ultra-high sensitivity CCD (Charge Coupled Device) has been introduced (Non-Patent Document 2), and a method using a photon counter equipped with a photomultiplier tube (PMT) has also been introduced (Non-Patent Document 3). [Prior art documents] [Non-patent literature]

[0007] [Non-Patent Document 1] Photochemistry and Photobiology Vol. 40, No. 4, pp. 485-494, 1984 [Non-Patent Document 2] Electrochemistry, 82(4), 294-298 (2014) [Non-Patent Document 3] J Photochem Photobiol B. 2014 Oct 5;139:63-70. doi: 10.1016 / j.jphotobiol.2013.10.003. Epub 2013 Oct 26. [Non-Patent Document 4] Medical science vol. 68, No. 8 1998 [Non-Patent Document 5] Journal of the Japanese Dermatological Society: (2008) 118(1) 17-21 Summary of the Invention [Problem to be solved by the invention]

[0008] UPE is a weak light emitted from living organisms and is a luminous phenomenon associated with biochemical reactions in the body. It has been measured locally. However, since UPE is a very weak luminous phenomenon, it has been difficult to measure UPE over a wide area such as the entire face. In addition, since the skin is constantly exposed to ultraviolet light in daily life, the impact of delayed luminescence based on external stimuli such as ultraviolet light is large, but the impact of only biochemical reactions on a wide area of ​​skin by excluding external stimuli has not been investigated. [Means for solving the problem]

[0009] The inventors found that by undergoing an acclimation process in a darkroom, the influence of external stimuli can be eliminated, and that only UPE, which is generated as a result of biochemical reactions that occur naturally in the skin, can be detected over a wide area such as the entire face. By using UPE imaging over the entire face, the inventors found that the detected UPE intensity varies greatly for each part of the face, and that the detected UPE intensity represents the oxidation state of the skin for each part of the face, leading to the present invention.

[0010] Specifically, the present invention relates to: [1] A process of acclimatizing the entire face in a dark room; Measuring the UPE intensity of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A process for determining the skin condition of each part based on the UPE strength of each part. A method for determining a skin condition, comprising: [2] The method according to item 1, wherein the step of determining the skin condition comprises normalizing the UPE intensity for each part with the UPE intensity of a control part and comparing it with a threshold value. [3] The method according to item 2, wherein the control area is a non-exposed area or a chin area. [4] The method according to any one of items 1 to 3, wherein the skin condition is an oxidation level of the skin. [5] The method according to any one of items 1 to 4, wherein the part is the upper eyelid or the corner of the eye, and the skin condition is a skin aging condition. [6] The method according to any one of items 1 to 4, wherein the skin condition is a wrinkled condition when the parts are the mouth, corners of the eyes, upper eyelids, lower eyelids, cheeks, between the eyebrows, and forehead. [7] The method according to any one of items 1 to 4, wherein the part is selected from the group consisting of the corners of the eyes, the cheeks, the forehead, and the nose, and the skin problem is blemishes. [8] A method for predicting the occurrence of future skin problems in a subject with healthy skin, comprising: A process of acclimatizing the entire face in a dark room; Measuring the UPE intensity of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A process to predict future skin troubles in each part based on the UPE strength of each part The method comprising: [9] The method according to item 8, wherein the step of predicting the occurrence of future skin problems includes normalizing the UPE intensity for each part with the UPE intensity of a control part and comparing it with a threshold value.

[10] The method according to item 9, wherein the control area is a non-exposed area or a chin area.

[11] The method according to any one of items 8 to 10, wherein the part is selected from the group consisting of the area around the mouth, the corners of the eyes, the upper eyelid, the lower eyelid, the cheeks, the space between the eyebrows, and the forehead, and the skin problem is wrinkles.

[12] The method according to any one of items 8 to 10, wherein the part is selected from the group consisting of the corners of the eyes, the cheeks, the forehead, and the nose, and the skin problem is blemishes.

[13] A process of acclimatizing the whole face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; A step of determining the amount of porphyrin in each part based on the UPE intensity of each part. A method for estimating the amount of porphyrin in the skin, comprising:

[14] The method according to Item 13, wherein the step of determining the amount of porphyrin is based on a graph showing the relationship between the amount of porphyrin in each part and UPE intensity.

[15] The method according to item 13 or 14, wherein the part is a cheek, a nose, a lower eyelid, between the eyebrows, or a forehead.

[16] A skin care pack containing a predetermined antioxidant component at a predetermined concentration determined for each part of the face in an area corresponding to each part of the face, The skin care pack, wherein the ingredients and their concentrations are determined for each part based on the UPE strength of each part of the face.

[17] The skin care pack according to item 16, wherein the UPE intensity for each facial part is determined by UPE imaging of the entire face in a darkroom or by a photocounter.

[18] A process of acclimatizing the whole face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter; A process of determining the cosmetic ingredient and its concentration suitable for each part based on the UPE strength of each part; providing a skin care pack containing ingredients determined for each part at determined concentrations in an area of ​​the skin care pack corresponding to the part; A method for producing a skin care pack comprising:

[19] A process of acclimatizing the whole face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; A process for determining the appropriate skin care and sun care products and their application methods for each part based on the UPE strength of each part; and a method for providing a skin care and / or sun care product and a method for using the same.

[20] The method according to item 19, further comprising a step of normalizing the UPE intensity for each part with the UPE intensity of a control part.

[21] The method according to item 20, wherein the control area is a non-exposed area or a chin area.

[22] The method according to any one of items 19 to 21, wherein the step of determining skin care and sun care products and their usage methods is based on a correspondence relationship between skin care and sun care products and their usage methods according to UPE strength. Effect of the Invention

[0011] According to the present invention, the skin condition of each facial part can be determined. [Brief description of the drawings]

[0012] [Figure 1] Figure 1 shows the classification of facial features for UPE strength analysis. [Diagram 2] FIG. 2 is a photograph showing the photon generation intensity of the entire face captured by UPE imaging. [Diagram 3] Figure 3 is a graph showing the average UPE intensity across all age groups for each part of the face (forehead, between the eyebrows, corners of the eyes, upper eyelids, lower eyelids, nose, nose ridge, under the nose, cheeks, lips, and chin). [Figure 4] FIG. 4 shows the UPE intensity of other parts of the body, with the UPE intensity of the jaw set at 100. [Diagram 5] FIG. 5 is a graph showing the correlation between UPE intensity and skin aging state in the upper eyelid and corner of the eye. [Figure 6] FIG. 6 is a graph showing the correlation between UPE intensity and the number of wrinkles per area in the area from the lower eyelid to the corner of the eye for subjects in their 20s to 40s. [Figure 7] FIG. 7 is a graph showing the correlation between UPE intensity in the cheek, nose, lower eyelid, glabella, and forehead and the number of porphyrins per region. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] The present invention relates to a method for determining skin condition using full face UPE imaging or a photocounter, specifically comprising the following steps: A process of acclimatizing the entire face in a dark room; Measuring the UPE of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A process for determining the skin condition of each part based on the UPE strength of each part. The method of the present invention allows the skin condition of each part to be determined individually. In the present invention, the skin condition refers to the oxidation level of the skin. Skin troubles such as wrinkles, age spots, and acne can be determined according to the facial parts based on the oxidation level of the skin.

[0014] The acclimation step in a dark room can eliminate the influence of delayed luminescence caused by ultraviolet light. The acclimation time can be selected from 5 minutes or more, more preferably 10 minutes or more, and even more preferably 15 minutes or more, from the viewpoint of eliminating the influence of delayed luminescence caused by ultraviolet light. From the viewpoint of simplifying the method, the acclimation time is usually within 1 hour, more preferably 15 minutes or less. In order to avoid external influences, acclimation can also be performed in a double dark room. The UPE intensity when the influence of delayed luminescence caused by ultraviolet light is eliminated reflects the luminescence phenomenon associated with biochemical reactions in the body accompanied by the generation of active oxygen and free radicals. In the skin, the higher the biochemical reaction, the higher the oxidation level, so the UPE intensity measured after the acclimation step indicates the oxidation level of the skin (Non-Patent Document 4: Medical Instruments, vol. 68, No. 8 1998).

[0015] A charge-coupled device camera can be used as the imaging device for UPE imaging. The charge-coupled device camera is preferably a cooled CCD camera. By using the imaging device for UPE imaging in a darkroom, the UPE can be measured for the entire face. Examples of the parts of the face include the forehead, glabella, nose, under the nose, lips, chin, corners of the eyes, upper eyelids, lower eyelids, cheeks, and nose ridge, and can be further classified into right and left areas (Figure 1). Also, instead of the charge-coupled device camera, a photon counter equipped with a photomultiplier tube (PMT) can be used to measure the UPE intensity for each part. It was shown that the UPE intensity varies greatly depending on the part of the face (Figure 2). The high UPE intensity was observed in the nose, nose ridge, and eyebrows, indicating that the oxidation level is high in these parts. Although not intending to be limited by theory, it is known that these parts have a high lipid production, and it is believed that stress due to lipid peroxides contributes to the oxidation level.

[0016] The UPE intensity of each part can be standardized based on the UPE intensity of a control part. As the control part, a non-exposed part such as an arm, abdomen, or back can be used, or any part of the face can be used. As an example, the chin can be used from the viewpoint of using a part of the face with low UPE intensity. By using a non-exposed part, the effect of ultraviolet rays in daily life can be investigated.

[0017] It is known that reactive oxygen in the skin affects cells of the epidermis and dermis. In keratinocytes present in the epidermis, the division rate is reduced due to the influence of reactive oxygen, resulting in thinning of the stratum corneum. In addition, melanocytes present in the epidermis are activated by reactive oxygen, and the amount of melanin produced increases. As a result, the possibility of developing spots increases in areas with a large amount of reactive oxygen. In fibroblasts in the dermis, the division rate is reduced due to the influence of reactive oxygen, and the production of interstitial components such as collagen and elastin is reduced. In addition, matrix metalloproteinase (MMP) is activated due to the influence of reactive oxygen, which destroys the dermal matrix and forms wrinkles. As a result, the influence of reactive oxygen can cause wrinkles and sagging (Non-Patent Document 5: Journal of the Japanese Dermatological Association: (2008) 118 (1) 17-21).

[0018] As mentioned above, active oxygen is closely related to the occurrence of age spots and wrinkles, but the areas where they are likely to occur differ. Age spots can appear anywhere on the face, but the corners of the eyes, cheeks, forehead, and nose are particularly common areas for age spots. Therefore, high UPE intensity in these areas is related to the occurrence of age spots.

[0019] On the other hand, wrinkles tend to form in areas of skin movement, particularly around the mouth, corners of the eyes, between the eyebrows, cheeks, forehead, upper eyelids, and lower eyelids. Therefore, high UPE intensity in these areas is associated with the development of wrinkles.

[0020] If a healthy subject who does not currently have wrinkles or age spots has a high UPE, it can be said that the risk of developing wrinkles or age spots in the future is high. Therefore, in a healthy subject who does not currently have wrinkles or age spots, by detecting the amount of UPE in a region where age spots or wrinkles frequently occur, it is possible to predict age spots or wrinkles. Therefore, in another aspect, the present invention relates to a method for predicting the occurrence of future skin troubles in subjects with healthy skin conditions. The method for predicting the occurrence of future skin troubles specifically includes the following steps: A process of acclimatizing the entire face in a dark room; Measuring the UPE of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A process to predict future skin troubles in each part based on the UPE strength of each part Includes.

[0021] The step of predicting the occurrence of future skin troubles can be performed by specifically comparing the measured UPE intensity for each part with a threshold value. A plurality of threshold values ​​can be set for each part, and the possibility of trouble occurrence can be predicted. As an example, when the UPE intensity at the corner of the eye is 2 or more, more preferably 2.5 or more, and even more preferably 3.0 or more, it indicates that there is a high possibility of future wrinkle formation, and the higher the threshold value, the higher the possibility of wrinkle formation.

[0022] Skin troubles predicted by UPE strength vary depending on the part of the skin. Areas where blemishes frequently occur are the corners of the eyes, lower eyelids, cheeks, and nose, and skin troubles in these areas result in blemishes. On the other hand, areas where wrinkles frequently occur are the mouth, corners of the eyes, cheeks, between the eyebrows, forehead, upper eyelids, and lower eyelids, and skin troubles in these areas result in wrinkles.

[0023] The UPE intensity for each part can also be standardized by the UPE intensity of a control part. Standardization using a control part can eliminate the influence of constitution. For example, when the chin is used as a control, it is useful in that it can eliminate the influence of an individual's constitution, such as high or low UPE amount, and identify the parts of the face that are prone to skin trouble.

[0024] Furthermore, the UPE amount in the cheeks, nose, lower eyelids, glabella, and forehead was correlated with the porphyrin amount (FIG. 7). Therefore, in a further aspect of the present invention, the present invention relates to a method for estimating the porphyrin amount in the skin. In the method for estimating the porphyrin amount in the skin, the porphyrin amount in each part can be determined based on the UPE amount for each part measured by performing UPE imaging or photocounting of the entire face in a dark room. More specifically, the porphyrin amount in each part can be determined based on a graph showing the relationship between the UPE amount and the porphyrin amount for each part. Porphyrin in the skin is mainly produced by Propionibacterium acnes. Therefore, by determining the porphyrin amount, the likelihood of acne can also be determined. For example, when the UPE intensity is 2.5 or more, more preferably 3.0 or more, in the cheeks, nose, lower eyelids, glabella, forehead, etc., it indicates that there is a high possibility of acne formation. For subjects who are judged to have a high possibility of acne formation, face washing instructions and information about products such as face washes containing acne prevention drugs can be provided.

[0025] In yet another aspect of the present invention, the present invention also relates to a skin care pack. The skin care pack is a beauty product that administers beauty ingredients to the skin by sticking it to the face. The skin care pack of the present invention relates to a skin care pack that contains a predetermined beauty ingredient determined for each part at a predetermined concentration in an area corresponding to each part of the face. Here, the beauty ingredient and its concentration determined for each part are determined based on the UPE amount for each part of the face. As a result, by blending a beauty ingredient with high efficacy in an area with a high UPE amount, such as the forehead and cheeks, a better beauty effect can be expected. In addition, the concentration of the beauty ingredient can be increased in an area with a high UPE amount, such as the forehead and cheeks. The UPE amount for each part of the face can be determined by UPE imaging of the entire face in a darkroom, and can also be determined using a photocounter.

[0026] An example of a cosmetic ingredient is an antioxidant ingredient. Examples of antioxidant ingredients that can be incorporated into cosmetics include vitamin C, vitamin E, β-carotene, astaxanthin, and derivatives thereof. In addition, some plant extracts are known to exhibit antioxidant effects. Examples of such plant extracts include aloe extract, ginkgo extract, oolong tea extract, turmeric extract, turmeric rhizome extract, asarum rhizome / root extract, edelweiss extract, olive leaf extract, hydrolyzed silk powder, artemisia capillaris extract, artemisia capillaris flower extract, licorice extract, rubus extract, gardenia extract, mulberry extract, gentiana root extract, geranium herb extract, tea extract, coffee extract, sesame extract, rice bran extract, Averrhoa carambola leaf extract, asarum extract, cherry leaf extract, white fungus polysaccharide, star fruit leaf extract, yarrow extract, sage extract, sage leaf extract, cnidium rhizome extract, corn extract, Houttuynia cordata extract, tomato extract, parsley extract, grape seed extract, safflower extract, peony extract, mulberry root bark extract, Madonna lily root extract, evening primrose seed extract, lily extract, rooibos extract, and rosemary extract.

[0027] The present invention may further relate to a method for producing a skin care pack. The method for producing a skin care pack of the present invention comprises the steps of: A process of acclimatizing the entire face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter; A process of determining the cosmetic ingredient and its concentration suitable for each part based on the UPE strength of each part; A step of providing a skin care pack containing a beauty ingredient determined for each part at a determined concentration in an area of ​​the skin care pack corresponding to the part. This makes it possible to provide skin care packs suited to the individual's skin.

[0028] In yet another aspect, the present invention relates to a method for providing a skin care and / or sun care product and a method for using the same, the method comprising the steps of: A process of acclimatizing the entire face in a dark room; measuring the UPE of each facial part by UPE imaging of the whole face or by a photocounter in a dark room; A process for determining the appropriate skin care and sun care products and their application methods for each part based on the UPE strength of each part; This method can provide information about skin care and / or sun care products suitable for an individual's skin, as well as information about usage methods suitable for an individual's skin.

[0029] In the present invention, UPE imaging is performed in a darkroom, and therefore can generally be performed at a department store or a store-type beauty counter. The method for determining skin condition, the method for predicting the occurrence of skin trouble, and the porphyrin estimation method of the present invention can be performed, and beauty counseling can be performed based on the results. In this case, a skin care pack can be manufactured and provided that is customized for each part according to the individual's skin condition and future possible troubles. In addition, skin care packs for people with various skin conditions and troubles can be patterned and prepared. In addition, based on the UPE strength of each part, a suitable skin care product and / or sun care product can be provided together with its usage method. As an example, for a subject with high UPE strength at the corners of the eyes, a beauty serum for the eyes containing a large amount of cosmetic ingredients with antioxidant effects can be provided together with information on the frequency of use. In addition, for a subject with high UPE strength on the cheeks, forehead, etc., a cosmetic such as a foundation containing an anti-UV agent can be provided together with the usage method. The skin care and sun care products and their usage methods are determined based on the correspondence between the skin care and sun care products and their usage methods according to the UPE strength. The correspondence between the UPE strength for each part and the corresponding skin care and sun care products and their usage methods may be recorded in advance in a correspondence table, for example. Such correspondence is determined by subdividing the skin care and sun care products and their usage methods for the UPE strength for each part according to the subject's age, location information, skin type, sex, etc., and appropriate skin care and sun care products and their usage methods can be provided based on the correspondence. EXAMPLES

[0030] Example 1: Full face UPE imaging Fifty women in their 20s to 60s were used as subjects. After washing their faces, they were allowed to acclimate for 15 minutes in a double darkroom, and their entire faces were photographed for 15 minutes using a cooled CCD camera (Spectral Instruments, Inc.: 600S Series). The photographic results are shown in Figure 2. The photon emission intensity was strong on the nose, near the nose, and between the eyebrows, indicating strong UPE generation. The amount of UPE was averaged for each area across all subjects, from their 20s to 60s (Figure 3).

[0031] To evaluate the level of facial oxidation based on the amount of UPE, each site was compared using the chin UPE as an index (Figure 4).

[0032] Example 2: Correlation between photon generation intensity and other indices When we investigated the correlation between photon generation intensity and age for the upper eyelid and outer corner of the eye, the correlation coefficients were r = 0.50 and 0.46, respectively (Figure 5).

[0033] Wrinkles around the eyes and lower eyelids were analyzed using VISIA Evolution (Canfield Scientific) for subjects in their 20s to 40s. The correlation between wrinkles and photon generation intensity was examined, and the correlation coefficient was r = 0.52 (Figure 6).

[0034] The amount of porphyrin on the face of all subjects (50 people) was measured using VISIA (Canfield Scientific). When the correlation between the measured amount of porphyrin and the photon generation intensity was examined, the correlation coefficient r was 0.44 (Figure 7).

Claims

1. A step of acclimating the entire face in a dark room; Measuring the UPE intensity of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A step of determining the skin condition of each part by standardizing the UPE intensity of each part with the UPE intensity of a control part and comparing the standardized UPE intensity of each part with a threshold value. A method for determining a skin condition, comprising:

2. The method according to claim 1 , wherein the control area is a non-exposed area or an area with low UPE intensity.

3. The method of claim 1 or 2, wherein the skin condition is the oxidation level of the skin.

4. The method according to any one of claims 1 to 3, wherein when the part is the upper eyelid or the corner of the eye, the skin condition is a skin aging condition.

5. The method according to any one of claims 1 to 3, wherein the skin condition is a wrinkled condition when the features are the mouth, the corners of the eyes, the upper eyelids, the lower eyelids, the cheeks, the space between the eyebrows, and the forehead.

6. The method according to any one of claims 1 to 3, wherein the part is selected from the group consisting of the corners of the eyes, the cheeks, the forehead, and the nose, and the skin trouble is blemishes.

7. A method for predicting future occurrence of skin trouble in a subject with healthy skin, comprising: A step of acclimating the entire face in a dark room; Measuring the UPE intensity of each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; and A process of predicting the occurrence of future skin troubles in each part by standardizing the UPE intensity for each part based on the UPE intensity for a control part and comparing the standardized UPE intensity for each part with a threshold value. The method comprising:

8. The method according to claim 7, wherein the step of predicting the occurrence of future skin problems includes normalizing the UPE intensity for each part by the UPE intensity of a control part and comparing it with a threshold value.

9. The method according to claim 8 , wherein the control area is a non-exposed area or an area with low UPE intensity.

10. The method according to any one of claims 7 to 9, wherein the part is selected from the group consisting of the mouth, the corners of the eyes, the upper eyelids, the lower eyelids, the cheeks, the space between the eyebrows, and the forehead, and the skin trouble is wrinkles.

11. The method according to any one of claims 7 to 9, wherein the part is selected from the group consisting of the corners of the eyes, the cheeks, the forehead, and the nose, and the skin trouble is blemishes.

12. A step of acclimating the entire face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; A step of normalizing the UPE intensity of each part with the UPE intensity of a control part, and determining the amount of porphyrin in each part by comparing the normalized UPE intensity of each part with a threshold value. A method for estimating the amount of porphyrin in the skin, comprising:

13. The method according to claim 12, wherein the step of determining the amount of porphyrin is based on a graph showing the relationship between the amount of porphyrin in each part and the UPE intensity.

14. The method according to claim 12 or 13, wherein the part is a cheek, a nose, a lower eyelid, between the eyebrows, or a forehead.

15. A step of acclimating the entire face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter; A step of standardizing the UPE intensity for each part based on the UPE intensity for a control part, and comparing the standardized UPE intensity for each part with a threshold value to determine a cosmetic ingredient and its concentration suitable for each part; providing a skin care pack containing ingredients determined for each part at determined concentrations in an area of ​​the skin care pack corresponding to the part; A method for producing a skin care pack comprising:

16. A step of acclimating the entire face in a dark room; measuring UPE intensity for each part of the face by UPE imaging of the whole face or by a photocounter in a dark room; A process of standardizing the UPE intensity for each part based on the UPE intensity for a control part, and comparing the standardized UPE intensity for each part with a threshold value to determine skin care and sun care products suitable for each part and their usage methods; and a method for providing a skin care and / or sun care product and a method for using the same.

17. The method of claim 16, further comprising the step of normalizing the UPE intensity for each part with the UPE intensity of a control part.

18. The method according to claim 17, wherein the control area is a non-exposed area or an area with low UPE intensity.

19. The method according to any one of claims 16 to 18, wherein the step of determining the skin care and sun care products and their usage methods is based on a correspondence relationship between the skin care and sun care products and their usage methods according to the UPE strength.

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