Insect ingredients containing ingredients

Abstract

To provide a composition containing an insect component with a novel antioxidative action.SOLUTION: The present invention provides an antioxidant for the kidneys that contains a cricket component.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to insect-containing compositions. [Background technology]

[0002] Humans have eaten insects since ancient times, and currently, more than 2,000 species of insects are commonly eaten by approximately 2 billion people in Asia, Central and South America, Africa, and other regions. Target insects are diverse, and examples include Hemiptera (e.g., water bugs, stink bugs, cicadas), Coleoptera (e.g., diving beetles, water beetles, jewel beetles, mealworms, longhorn beetles, scarab beetles, stag beetles, weevils, European cockroach beetles), Lepidoptera (silkworm moths, mountain saturday moths, hawk moths, carpenter moth larvae, noctuid moth larvae, bat moths, Japanese cicada moths, pyralid moths, and common moths), Hymenoptera (bees, ants, weaver ants, leaf-cutter ants, and honeybee ants), Orthoptera (locusts, grasshoppers, crickets, and mole crickets), Diptera (flies, cheese flies, right-handed flies, mosquitoes, midges, and cow flies), and others (stoneflies, caddisflies, dragonflies, termites, and cockroaches).

[0003] Here, it is known that insect extracts have antioxidant properties (e.g., Non-Patent Document 1). When active oxygen increases and a state of oxidative stress occurs, it is said to lead to various diseases such as arteriosclerosis, cancer, and decreased immune function. In order to prevent such oxidation in the human body, foods and beverages with antioxidant properties are effective. [Prior art documents] [Non-patent literature]

[0004] [Non-Patent Document 1] Oleoscience Vol. 22, No. 4 (2022) DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a composition containing an insect component having a useful antioxidant effect. [Means for solving the problem]

[0006] The present inventors have verified the antioxidant properties of various insects from various angles, and have found that cricket components have antioxidant properties specific to the kidney, leading to the completion of the present invention. Specifically, the present invention is as follows. The present invention (1) is a renal antioxidant containing a cricket component. The present invention (2) relates to the antioxidant of the above-mentioned invention (1), wherein the cricket component is a powder or an extract. The present invention (3) is a functional food, drink, or supplement comprising the antioxidant of the present invention (1) or (2). Effect of the Invention

[0007] According to the present invention, a composition containing an insect component having a useful antioxidant effect can be provided. [Brief description of the drawings]

[0008] [Figure 1] FIG. 1 shows the results of evaluation of total oxidative activity (TAC) in various organs of ICR mice in an example. [Diagram 2] FIG. 2 shows the results of evaluating superoxide dismutase (SOD) activity in various organs of ICR mice in an example. [Diagram 3] FIG. 3 shows the results of evaluating changes in TAC or SOD activity in the kidney of ICR mice according to the dose of cricket powder (HCP) administered in an example. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] The present invention relates to an antioxidant for the kidneys, which contains a cricket component. The antioxidant for the kidneys according to the present invention will be described in detail below.

[0010] <Kidney antioxidant> The renal antioxidant according to the present embodiment contains a cricket component. Here, as long as it contains a cricket component, there is no particular limitation, and typically, it may be cricket itself, cricket powder, a compressed product of the powder, or a cricket extract.

[0011] Here, the cricket as the raw material is not particularly limited as long as it is an insect of the Gryllidae family, and examples thereof include the European house cricket, the two-spotted cricket, the emma cricket, the three-corner cricket, the cockroach cricket, the tsuzuresa seko cricket, etc. Among these, the European house cricket is preferable.

[0012] ≪Manufacturing method≫ The agent according to this embodiment is preferably a powder or extract. Here, the method of powdering crickets is not particularly limited, and may be, for example, a method of boiling and drying crickets, and then pulverizing them into powder. In addition, the method of extracting crickets is also not particularly limited, and may be, for example, a vacuum low-temperature extraction method.

[0013] ≪Applications≫ The agent according to this embodiment is for kidney use. The agent according to this embodiment may be in the form of a functional food or drink or in the form of a supplement, either alone or together with other ingredients. Here, the application method is, for example, to orally ingest the agent once a day at a maximum dose of 3000 mg / kg. EXAMPLES

[0014] The present invention will be described in detail below with reference to examples, although the present invention is not limited to the following examples.

[0015] ICR mice were acclimated for one week in an animal house, and their health status was checked and their weights were measured. They were divided into three groups (3-4 mice / group): a 0.9% saline (SA)-treated group (control), a house cricket powder (HCP)-treated group (3000 mg / kg), and a positive control group (Astaxanthin (AXT: 10 mg / kg)-treated group. The mice were orally administered SA, HCP, or AXT for 14 consecutive days, and their weights, abnormal conditions, and deaths were checked daily. After administration on the 14th day, the mice were fasted for 16 hours, and deep anesthesia was induced by intraperitoneal administration of 200 mg / kg of sodium secobarbital solution. After complete blood collection, the brain, eyeballs, kidneys, gastrocnemius, and soleus muscles were removed. The removed organs were adjusted to 0.5 g / ml (wet weight) with SA and crushed using a bead smasher. The supernatant was collected as a water-soluble extract sample by centrifugation at 8000 rpm at 4°C. The total antioxidant activity (TAC) and superoxide dismutase (SOD) activity of the water-soluble extract sample from each organ were measured using a TAC assay kit and an SOD assay kit-WST. The TAC and SOD activities of each organ were calculated based on the activity of the control group as 100%.

[0016] As a result, a significant increase in TAC activity was observed in the kidneys of the HCP-administered group compared to the control group. Additionally, a significant increase in TAC activity was observed in the brain and kidneys of the AXT-administered group compared to the control or HCP-administered groups. On the other hand, no difference in TAC activity due to HCP administration was observed in the brain, eyeball, soleus muscle, gastrocnemius muscle, or serum (Figure 1). Next, no difference in SOD activity was observed in any organs of the HCP-administered group compared to the control group (Figure 2). One-way ANOVA was used for statistical testing, with p < 0.05 indicating a significant difference, and the number of mice used is shown in the columns of the graph.

[0017] ICR mice were acclimated for one week in an animal house, and their health was checked and their weights were measured. They were then divided into four groups (5-6 mice / group): a 0.9% SA-administered group (control), and HCP: 300, 1000, and 3000 mg / kg-administered groups. The mice were orally administered SA or HCP for 14 consecutive days, and their weights, abnormal conditions, and deaths were checked daily. After administration on the 14th day, the mice were fasted for 16 hours, and deep anesthesia was induced by intraperitoneal administration of 200 mg / kg of secobarbital sodium solution. After complete blood collection, the kidneys were removed. The removed kidneys were adjusted to 0.5 g / ml (wet weight) with SA and crushed using a bead smasher. The mixture was then centrifuged at 4°C and 8000 rpm for 1 hour, and the supernatant was used as a water-soluble extract sample. The TAC and SOD activities of the aqueous kidney extract samples were measured, and the specific activity of each group was calculated by setting the activity of the control group as 100%.

[0018] As a result, in the kidney, the TAC assay showed a significant increase in TAC in all HCP-administered groups compared to the control group, with the highest increase in activity observed in the HCP: 1000 mg / kg group. On the other hand, the SOD assay showed a significant increase in SOD activity only in the HCP: 1000 mg / kg group compared to the control group (Figure 3). One-way ANOVA was used for statistical testing, with p < 0.05 indicating significant differences, and the number of mice used is shown in the graph columns.

Claims

1. A renal antioxidant comprising powder or extract of crickets of the genus Meretrix.

2. A functional food, drink, or supplement for antioxidation of the kidneys, comprising the renal antioxidant according to claim 1.

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