How to determine genetic predisposition to rough skin

Abstract

To provide means for determining an individual's genetic predisposition of rough skin accurately and readily.SOLUTION: A method for determining genetic predisposition of rough skin includes the steps of: detecting an allele at a single nucleotide polymorphism (SNP) site of a base sequence having a specific sequence for a DNA-containing sample taken from a subject; and determining that the subject has genetic predisposition of rough skin when at least one base of the detected allele is a risk allele.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a method for determining a genetic predisposition to rough skin, and more particularly to a method for determining a genetic predisposition to rough skin by detecting a single nucleotide polymorphism (SNP) associated with rough skin, and a kit used in said method. [Background technology]

[0002] The skin is an organ that covers the entire human body, and is composed of three layers from the outside: the epidermis, dermis, and subcutaneous tissue. The epidermis, which is the outermost layer, is further divided into four layers: the basal layer, the spinous layer, the granular layer, and the stratum corneum (Non-Patent Document 1). Of these, the stratum corneum, which is the outermost layer, has a barrier function that protects the body from external stimuli and at the same time keeps the moisture inside the skin constant. Rough skin is a type of skin trouble and is a general term for a state in which the surface morphology of the skin is disturbed. In terms of appearance, it is characterized by dryness, peeling, and disturbance of the texture of the stratum corneum of the skin. It is thought that various factors such as ultraviolet rays, dryness, temperature, mental state, and quality and quantity of sleep are involved in rough skin.

[0003] Known methods for evaluating the condition of rough skin include a method that uses various proteins in stratum corneum cells collected from a subject as indicators (Patent Document 1 and Patent Document 2) and a method that analyzes the rate of desquamation in skin images (Patent Document 3). However, these methods evaluate the subject's current skin condition, and do not evaluate the subject's inherent susceptibility to rough skin (genetic predisposition). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] WO2006 / 098523 publication [Patent Document 2] JP 2014-41043 A [Patent Document 3] JP 2018-196428 A Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a means for accurately and simply determining an individual's genetic predisposition to rough skin. [Means for solving the problem]

[0006] The inventors conducted a comprehensive analysis of single nucleotide polymorphisms (SNPs) using genome-wide association studies (GWAS) targeting sample donors of different ages and genders, and discovered single nucleotide polymorphisms (SNPs) and genes associated with a genetic predisposition to rough skin (susceptibility to rough skin), thereby completing the present invention.

[0007] That is, the present invention includes the following inventions. [1] A method for determining a genetic predisposition to rough skin, comprising the steps of: detecting alleles of one or more of the following single nucleotide polymorphisms (SNPs) (a1) to (a82) in a DNA-containing sample collected from a subject; and determining that the subject has a genetic predisposition to rough skin if at least one of the bases of the detected allele is a risk allele. (a1) SNP at the 101st base of the base sequence shown in SEQ ID NO: 1 (SNP: SNP identified by ID rs557778) (a2) SNP at the 101st base of the base sequence shown in SEQ ID NO: 2 (SNP: SNP identified by ID rs555337) (a3) SNP at the 101st base of the base sequence shown in SEQ ID NO: 3 (SNP: SNP identified by ID rs666003) (a4) SNP at the 101st base of the base sequence shown in SEQ ID NO: 4 (SNP: SNP identified by ID rs666460) (a5) SNP at the 101st base of the base sequence shown in SEQ ID NO: 5 (SNP: SNP identified by ID rs666461) (a6) SNP at the 101st base of the base sequence shown in SEQ ID NO: 6 (SNP: SNP identified by ID rs526733) (a7) SNP at the 101st base of the base sequence shown in SEQ ID NO: 7 (SNP: SNP identified by ID rs525842) (a8) SNP at the 101st base of the base sequence shown in SEQ ID NO: 8 (SNP: SNP identified by ID rs4915051) (a9) SNP at the 101st base of the base sequence shown in SEQ ID NO: 9 (SNP: SNP identified by ID rs680867) (a10) SNP at the 101st base of the base sequence shown in SEQ ID NO: 10 (SNP: SNP identified by ID rs499513) (a11) SNP at the 101st base of the base sequence shown in SEQ ID NO: 11 (SNP: SNP identified by ID rs684624) (a12) SNP at the 101st base of the base sequence shown in SEQ ID NO: 12 (SNP: SNP identified by ID rs494757) (a13) SNP at the 101st base of the base sequence shown in SEQ ID NO: 13 (SNP: SNP identified by ID rs494696) (a14) SNP at the 101st base of the base sequence shown in SEQ ID NO: 14 (SNP: SNP identified by ID rs580995) (a15) SNP at the 101st base of the base sequence shown in SEQ ID NO: 15 (SNP: SNP identified by ID rs515202) (a16) SNP at the 101st base of the base sequence shown in SEQ ID NO: 16 (SNP: SNP identified by ID rs566923) (a17) SNP at the 101st base of the base sequence shown in SEQ ID NO: 17 (SNP: SNP identified by ID rs560683) (a18) SNP at the 101st base of the base sequence shown in SEQ ID NO: 18 (SNP: SNP identified by ID rs560655) (a19) SNP at the 101st base of the base sequence shown in SEQ ID NO: 19 (SNP: SNP identified by ID rs663391) (a20) SNP at the 101st base of the base sequence shown in SEQ ID NO: 20 (SNP: SNP identified by ID rs663431) (a21) SNP at the 101st base of the base sequence shown in SEQ ID NO: 21 (SNP: SNP identified by ID rs12032660) (a22) SNP at the 101st base of the base sequence shown in SEQ ID NO: 22 (SNP: SNP identified by ID rs12035053) (a23) SNP at the 101st base of the base sequence shown in SEQ ID NO: 23 (SNP: SNP identified by ID rs11185132) (a24) SNP at the 101st base of the base sequence shown in SEQ ID NO: 24 (SNP: SNP identified by ID rs6665028) (a25) SNP at the 101st base of the base sequence shown in SEQ ID NO: 25 (SNP: SNP identified by ID rs6702592) (a26) SNP at the 101st base of the base sequence shown in SEQ ID NO: 26 (SNP: SNP identified by ID rs534891) (a27) SNP at the 101st base of the base sequence shown in SEQ ID NO: 27 (SNP: SNP identified by ID rs666070) (a28) SNP at the 101st base of the base sequence shown in SEQ ID NO: 28 (SNP: SNP identified by ID rs666956) (a29) SNP at the 101st base of the base sequence shown in SEQ ID NO: 29 (SNP: SNP identified by ID rs1752681) (a30) SNP at the 101st base of the base sequence shown in SEQ ID NO: 30 (SNP: SNP identified by ID rs505822) (a31) SNP at the 101st base of the base sequence shown in SEQ ID NO: 31 (SNP: SNP identified by ID rs504881) (a32) SNP at the 101st base of the base sequence shown in SEQ ID NO: 32 (SNP: SNP identified by ID rs504806) (a33) SNP at the 101st base of the base sequence shown in SEQ ID NO: 33 (SNP: SNP identified by ID rs501057) (a34) SNP at the 101st base of the base sequence shown in SEQ ID NO: 34 (SNP: SNP identified by ID rs499227) (a35) SNP at the 101st base of the base sequence shown in SEQ ID NO: 35 (SNP: SNP identified by ID rs586180) (a36) SNP at the 101st base of the base sequence shown in SEQ ID NO: 36 (SNP: SNP identified by ID rs476157) (a37) SNP at the 101st base of the base sequence shown in SEQ ID NO: 37 (SNP: SNP identified by ID rs475072) (a38) SNP at the 101st base of the base sequence shown in SEQ ID NO: 38 (SNP: SNP identified by ID rs473420) (a39) SNP at the 101st base of the base sequence shown in SEQ ID NO: 39 (SNP: SNP identified by ID rs472527) (a40) SNP at the 101st base of the base sequence shown in SEQ ID NO: 40 (SNP: SNP identified by ID rs580075) (a41) SNP at the 101st base of the base sequence shown in SEQ ID NO: 41 (SNP: SNP identified by ID rs549903) (a42) SNP at the 101st base of the base sequence shown in SEQ ID NO: 42 (SNP: SNP identified by ID rs10785826) (a43) SNP at the 101st base of the base sequence shown in SEQ ID NO: 43 (SNP: SNP identified by ID rs494152) (a44) SNP at the 101st base of the base sequence shown in SEQ ID NO: 44 (SNP: SNP identified by ID rs643908) (a45) SNP at the 101st base of the base sequence shown in SEQ ID NO: 45 (SNP: SNP identified by ID rs11811454) (a46) SNP at the 101st base of the base sequence shown in SEQ ID NO: 46 (SNP: SNP identified by ID rs61819207) (a47) SNP at the 101st base of the base sequence shown in SEQ ID NO: 47 (SNP: SNP identified by ID rs289852) (a48) SNP at the 101st base of the base sequence shown in SEQ ID NO: 48 (SNP: SNP identified by ID rs1056726) (a49) SNP at the 101st base of the base sequence shown in SEQ ID NO: 49 (SNP: SNP identified by ID rs149819999) (a50) SNP at the 101st base of the base sequence shown in SEQ ID NO: 50 (SNP: SNP identified by ID rs60254106) (a51) SNP at the 101st base of the base sequence shown in SEQ ID NO: 51 (SNP: SNP identified by ID rs2173313) (a52) SNP at the 101st base of the base sequence shown in SEQ ID NO: 52 (SNP: SNP identified by ID rs12209775) (a53) SNP at the 101st base of the base sequence shown in SEQ ID NO: 53 (SNP: SNP identified by ID rs12211110) (a54) SNP at the 101st base of the base sequence shown in SEQ ID NO: 54 (SNP: SNP identified by ID rs7738045) (a55) SNP at the 101st base of the base sequence shown in SEQ ID NO: 55 (SNP: SNP identified by ID rs1937834) (a56) SNP at the 101st base of the base sequence shown in SEQ ID NO: 56 (SNP: SNP identified by ID rs75741813) (a57) SNP at the 101st base of the base sequence shown in SEQ ID NO: 57 (SNP: SNP identified by ID rs74686576) (a58) SNP at the 101st base of the base sequence shown in SEQ ID NO: 58 (SNP: SNP identified by ID rs74415127) (a59) SNP at the 101st base of the base sequence shown in SEQ ID NO: 59 (SNP: SNP identified by ID rs77750385) (a60) SNP at the 101st base of the base sequence shown in SEQ ID NO: 60 (SNP: SNP identified by ID rs76145983) (a61) SNP at the 101st base of the base sequence shown in SEQ ID NO: 61 (SNP: SNP identified by ID rs75155123) (a62) SNP at the 101st base of the base sequence shown in SEQ ID NO: 62 (SNP: SNP identified by ID rs74302809) (a63) SNP at the 101st base of the base sequence shown in SEQ ID NO: 63 (SNP: SNP identified by ID rs111523535) (a64) SNP at the 101st base of the base sequence shown in SEQ ID NO: 64 (SNP: SNP identified by ID rs199627764) (a65) SNP at the 101st base of the base sequence shown in SEQ ID NO: 65 (SNP: SNP identified by ID rs79187933) (a66) SNP at the 101st base of the base sequence shown in SEQ ID NO: 66 (SNP: SNP identified by ID rs77784037) (a67) SNP at the 101st base of the base sequence shown in SEQ ID NO: 67 (SNP: SNP identified by ID rs78530814) (a68) SNP at the 101st base of the base sequence shown in SEQ ID NO: 68 (SNP: SNP identified by ID rs4739577) (a69) SNP at the 101st base of the base sequence shown in SEQ ID NO: 69 (SNP: SNP identified by ID rs78484770) (a70) SNP at the 101st base of the base sequence shown in SEQ ID NO: 70 (SNP: SNP identified by ID rs76611879) (a71) SNP at the 101st base of the base sequence shown in SEQ ID NO: 71 (SNP: SNP identified by ID rs80335309) (a72) SNP at the 101st base of the base sequence shown in SEQ ID NO: 72 (SNP: SNP identified by ID rs75558330) (a73) SNP at the 101st base of the base sequence shown in SEQ ID NO: 73 (SNP: SNP identified by ID rs76151608) (a74) SNP at the 101st base of the base sequence shown in SEQ ID NO: 74 (SNP: SNP identified by ID rs74407439) (a75) SNP at the 101st base of the base sequence shown in SEQ ID NO: 75 (SNP: SNP identified by ID rs112276204) (a76) SNP at the 101st base of the base sequence shown in SEQ ID NO: 76 (SNP: SNP identified by ID rs75023163) (a77) SNP at the 101st base of the base sequence shown in SEQ ID NO: 77 (SNP: SNP identified by ID rs4739578) (a78) SNP at the 101st base of the base sequence shown in SEQ ID NO: 78 (SNP: SNP identified by ID rs4739579) (a79) SNP at the 101st base of the base sequence shown in SEQ ID NO: 79 (SNP: SNP identified by ID rs16882394) (a80) SNP at the 101st base of the base sequence shown in SEQ ID NO: 80 (SNP: SNP identified by ID rs77736544) (a81) SNP at the 101st base of the base sequence shown in SEQ ID NO: 81 (SNP: SNP identified by ID rs78585145) (a82) SNP at the 101st base of the base sequence shown in SEQ ID NO: 82 (SNP: SNP identified by ID rs16882399) [2] A method for determining a genetic predisposition to rough skin, comprising the steps of: detecting an allele of a single nucleotide polymorphism (SNP) associated with one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8 in a DNA-containing sample collected from a subject; and determining that the subject has a genetic predisposition to rough skin if at least one of the bases of the detected allele is a risk allele. [3] A method for providing cosmetics and / or food and beverages to a subject, comprising providing the subject with cosmetics and / or food and beverages having the effect of preventing and / or improving rough skin according to the degree of the subject's genetic predisposition to rough skin based on the results determined by the method described in [1] or [2]. [4] A kit for determining a genetic predisposition to rough skin, comprising a probe having a sequence of 10 or more bases including the 101st base in a base sequence shown in any one of SEQ ID NOs: 1 to 82, or a complementary sequence thereof, and / or a primer capable of amplifying a region including the 101st base in a base sequence shown in any one of SEQ ID NOs: 1 to 82. [5] A method for screening for an agent for preventing and / or improving rough skin, using changes in the expression of one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8 as an indicator. [6] A composition for preventing and / or improving rough skin, comprising a material that controls the expression of one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8. Effect of the Invention

[0008] According to the method of the present invention, by detecting the alleles of single nucleotide polymorphisms (SNPs) contained in genome-derived DNA present in a biological sample from a subject, it is possible to accurately and simply determine whether the subject has a genetic predisposition to rough skin (whether the subject is prone to rough skin). Therefore, based on the results of this determination, measures for preventing or improving rough skin can be taken early. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] 1. How to determine genetic predisposition to rough skin The method of the present invention for determining a genetic predisposition to rough skin comprises the steps of detecting alleles of one or more of the following single nucleotide polymorphisms (SNPs) (a1) to (a82) in a DNA-containing sample collected from a subject, and determining that the subject has a genetic predisposition to rough skin if at least one of the bases of the detected allele is a risk allele. (a1) SNP at the 101st base of the base sequence shown in SEQ ID NO: 1 (SNP: SNP identified by ID rs557778) (a2) SNP at the 101st base of the base sequence shown in SEQ ID NO: 2 (SNP: SNP identified by ID rs555337) (a3) SNP at the 101st base of the base sequence shown in SEQ ID NO: 3 (SNP: SNP identified by ID rs666003) (a4) SNP at the 101st base of the base sequence shown in SEQ ID NO: 4 (SNP: SNP identified by ID rs666460) (a5) SNP at the 101st base of the base sequence shown in SEQ ID NO: 5 (SNP: SNP identified by ID rs666461) (a6) SNP at the 101st base of the base sequence shown in SEQ ID NO: 6 (SNP: SNP identified by ID rs526733) (a7) SNP at the 101st base of the base sequence shown in SEQ ID NO: 7 (SNP: SNP identified by ID rs525842) (a8) SNP at the 101st base of the base sequence shown in SEQ ID NO: 8 (SNP: SNP identified by ID rs4915051) (a9) SNP at the 101st base of the base sequence shown in SEQ ID NO: 9 (SNP: SNP identified by ID rs680867) (a10) SNP at the 101st base of the base sequence shown in SEQ ID NO: 10 (SNP: SNP identified by ID rs499513) (a11) SNP at the 101st base of the base sequence shown in SEQ ID NO: 11 (SNP: SNP identified by ID rs684624) (a12) SNP at the 101st base of the base sequence shown in SEQ ID NO: 12 (SNP: SNP identified by ID rs494757) (a13) SNP at the 101st base of the base sequence shown in SEQ ID NO: 13 (SNP: SNP identified by ID rs494696) (a14) SNP at the 101st base of the base sequence shown in SEQ ID NO: 14 (SNP: SNP identified by ID rs580995) (a15) SNP at the 101st base of the base sequence shown in SEQ ID NO: 15 (SNP: SNP identified by ID rs515202) (a16) SNP at the 101st base of the base sequence shown in SEQ ID NO: 16 (SNP: SNP identified by ID rs566923) (a17) SNP at the 101st base of the base sequence shown in SEQ ID NO: 17 (SNP: SNP identified by ID rs560683) (a18) SNP at the 101st base of the base sequence shown in SEQ ID NO: 18 (SNP: SNP identified by ID rs560655) (a19) SNP at the 101st base of the base sequence shown in SEQ ID NO: 19 (SNP: SNP identified by ID rs663391) (a20) SNP at the 101st base of the base sequence shown in SEQ ID NO: 20 (SNP: SNP identified by ID rs663431) (a21) SNP at the 101st base of the base sequence shown in SEQ ID NO: 21 (SNP: SNP identified by ID rs12032660) (a22) SNP at the 101st base of the base sequence shown in SEQ ID NO: 22 (SNP: SNP identified by ID rs12035053) (a23) SNP at the 101st base of the base sequence shown in SEQ ID NO: 23 (SNP: SNP identified by ID rs11185132) (a24) SNP at the 101st base of the base sequence shown in SEQ ID NO: 24 (SNP: SNP identified by ID rs6665028) (a25) SNP at the 101st base of the base sequence shown in SEQ ID NO: 25 (SNP: SNP identified by ID rs6702592) (a26) SNP at the 101st base of the base sequence shown in SEQ ID NO: 26 (SNP: SNP identified by ID rs534891) (a27) SNP at the 101st base of the base sequence shown in SEQ ID NO: 27 (SNP: SNP identified by ID rs666070) (a28) SNP at the 101st base of the base sequence shown in SEQ ID NO: 28 (SNP: SNP identified by ID rs666956) (a29) SNP at the 101st base of the base sequence shown in SEQ ID NO: 29 (SNP: SNP identified by ID rs1752681) (a30) SNP at the 101st base of the base sequence shown in SEQ ID NO: 30 (SNP: SNP identified by ID rs505822) (a31) SNP at the 101st base of the base sequence shown in SEQ ID NO: 31 (SNP: SNP identified by ID rs504881) (a32) SNP at the 101st base of the base sequence shown in SEQ ID NO: 32 (SNP: SNP identified by ID rs504806) (a33) SNP at the 101st base of the base sequence shown in SEQ ID NO: 33 (SNP: SNP identified by ID rs501057) (a34) SNP at the 101st base of the base sequence shown in SEQ ID NO: 34 (SNP: SNP identified by ID rs499227) (a35) SNP at the 101st base of the base sequence shown in SEQ ID NO: 35 (SNP: SNP identified by ID rs586180) (a36) SNP at the 101st base of the base sequence shown in SEQ ID NO: 36 (SNP: SNP identified by ID rs476157) (a37) SNP at the 101st base of the base sequence shown in SEQ ID NO: 37 (SNP: SNP identified by ID rs475072) (a38) SNP at the 101st base of the base sequence shown in SEQ ID NO: 38 (SNP: SNP identified by ID rs473420) (a39) SNP at the 101st base of the base sequence shown in SEQ ID NO: 39 (SNP: SNP identified by ID rs472527) (a40) SNP at the 101st base of the base sequence shown in SEQ ID NO: 40 (SNP: SNP identified by ID rs580075) (a41) SNP at the 101st base of the base sequence shown in SEQ ID NO: 41 (SNP: SNP identified by ID rs549903) (a42) SNP at the 101st base of the base sequence shown in SEQ ID NO: 42 (SNP: SNP identified by ID rs10785826) (a43) SNP at the 101st base of the base sequence shown in SEQ ID NO: 43 (SNP: SNP identified by ID rs494152) (a44) SNP at the 101st base of the base sequence shown in SEQ ID NO: 44 (SNP: SNP identified by ID rs643908) (a45) SNP at the 101st base of the base sequence shown in SEQ ID NO: 45 (SNP: SNP identified by ID rs11811454) (a46) SNP at the 101st base of the base sequence shown in SEQ ID NO: 46 (SNP: SNP identified by ID rs61819207) (a47) SNP at the 101st base of the base sequence shown in SEQ ID NO: 47 (SNP: SNP identified by ID rs289852) (a48) SNP at the 101st base of the base sequence shown in SEQ ID NO: 48 (SNP: SNP identified by ID rs1056726) (a49) SNP at the 101st base of the base sequence shown in SEQ ID NO: 49 (SNP: SNP identified by ID rs149819999) (a50) SNP at the 101st base of the base sequence shown in SEQ ID NO: 50 (SNP: SNP identified by ID rs60254106) (a51) SNP at the 101st base of the base sequence shown in SEQ ID NO: 51 (SNP: SNP identified by ID rs2173313) (a52) SNP at the 101st base of the base sequence shown in SEQ ID NO: 52 (SNP: SNP identified by ID rs12209775) (a53) SNP at the 101st base of the base sequence shown in SEQ ID NO: 53 (SNP: SNP identified by ID rs12211110) (a54) SNP at the 101st base of the base sequence shown in SEQ ID NO: 54 (SNP: SNP identified by ID rs7738045) (a55) SNP at the 101st base of the base sequence shown in SEQ ID NO: 55 (SNP: SNP identified by ID rs1937834) (a56) SNP at the 101st base of the base sequence shown in SEQ ID NO: 56 (SNP: SNP identified by ID rs75741813) (a57) SNP at the 101st base of the base sequence shown in SEQ ID NO: 57 (SNP: SNP identified by ID rs74686576) (a58) SNP at the 101st base of the base sequence shown in SEQ ID NO: 58 (SNP: SNP identified by ID rs74415127) (a59) SNP at the 101st base of the base sequence shown in SEQ ID NO: 59 (SNP: SNP identified by ID rs77750385) (a60) SNP at the 101st base of the base sequence shown in SEQ ID NO: 60 (SNP: SNP identified by ID rs76145983) (a61) SNP at the 101st base of the base sequence shown in SEQ ID NO: 61 (SNP: SNP identified by ID rs75155123) (a62) SNP at the 101st base of the base sequence shown in SEQ ID NO: 62 (SNP: SNP identified by ID rs74302809) (a63) SNP at the 101st base of the base sequence shown in SEQ ID NO: 63 (SNP: SNP identified by ID rs111523535) (a64) SNP at the 101st base of the base sequence shown in SEQ ID NO: 64 (SNP: SNP identified by ID rs199627764) (a65) SNP at the 101st base of the base sequence shown in SEQ ID NO: 65 (SNP: SNP identified by ID rs79187933) (a66) SNP at the 101st base of the base sequence shown in SEQ ID NO: 66 (SNP: SNP identified by ID rs77784037) (a67) SNP at the 101st base of the base sequence shown in SEQ ID NO: 67 (SNP: SNP identified by ID rs78530814) (a68) SNP at the 101st base of the base sequence shown in SEQ ID NO: 68 (SNP: SNP identified by ID rs4739577) (a69) SNP at the 101st base of the base sequence shown in SEQ ID NO: 69 (SNP: SNP identified by ID rs78484770) (a70) SNP at the 101st base of the base sequence shown in SEQ ID NO: 70 (SNP: SNP identified by ID rs76611879) (a71) SNP at the 101st base of the base sequence shown in SEQ ID NO: 71 (SNP: SNP identified by ID rs80335309) (a72) SNP at the 101st base of the base sequence shown in SEQ ID NO: 72 (SNP: SNP identified by ID rs75558330) (a73) SNP at the 101st base of the base sequence shown in SEQ ID NO: 73 (SNP: SNP identified by ID rs76151608) (a74) SNP at the 101st base of the base sequence shown in SEQ ID NO: 74 (SNP: SNP identified by ID rs74407439) (a75) SNP at the 101st base of the base sequence shown in SEQ ID NO: 75 (SNP: SNP identified by IDrs112276204) (a76) SNP at the 101st base of the base sequence shown in SEQ ID NO: 76 (SNP: SNP identified by ID rs75023163) (a77) SNP at the 101st base of the base sequence shown in SEQ ID NO: 77 (SNP: SNP identified by ID rs4739578) (a78) SNP at the 101st base of the base sequence shown in SEQ ID NO: 78 (SNP: SNP identified by ID rs4739579) (a79) SNP at the 101st base of the base sequence shown in SEQ ID NO: 79 (SNP: SNP identified by ID rs16882394) (a80) SNP at the 101st base of the base sequence shown in SEQ ID NO: 80 (SNP: SNP identified by ID rs77736544) (a81) SNP at the 101st base of the base sequence shown in SEQ ID NO: 81 (SNP: SNP identified by ID rs78585145) (a82) SNP at the 101st base of the base sequence shown in SEQ ID NO: 82 (SNP: SNP identified by ID rs16882399)

[0010] The base sequences containing the above SNPs (a1) to (a82) (the numbers in [ ] represent SNPs) are shown in Table 1.

[0011] [Table 1] TIFF0007629628000002.tif248160TIFF0007629628000003.tif252161TIFF000 7629628000004.tif244160TIFF0007629628000005.tif244168TIFF0007629628 000006.tif246157TIFF0007629628000007.tif245157TIFF0007629628000008. tif245169TIFF0007629628000009.tif246159TIFF0007629628000010.tif62158

[0012] As shown in the results of genome-wide association studies (GWAS) in the Examples described later, the SNPs (a1) to (a82) above have a p-value of 1×10 -5 The results are less than 100 kb, suggesting a statistical association with a genetic predisposition to rough skin. In addition, VAV3, RBM43, NMI, TNFAIP6, and RNF8 shown in Table 2 below were identified as genes that are located in the vicinity (within 100 kb) of the above SNPs (a1) to (a82) specified in the present invention or whose expression is known in the literature to vary due to the SNPs. Thus, according to the present invention, a method for determining a genetic predisposition to rough skin is provided, which includes a step of detecting an allele of a single nucleotide polymorphism (SNP) associated with one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8 in a DNA-containing sample collected from a subject, and a step of determining that the subject has a genetic predisposition to rough skin when at least one base of the detected allele is a risk allele.

[0013] [Table 2]

[0014] A single nucleotide polymorphism (SNP, hereinafter sometimes referred to as "SNP") generally refers to a state in which the nucleotide sequence of a gene differs at only one location and the location of that difference. Moreover, a polymorphism generally refers to two or more alleles that exist at a frequency of 1% or more in a population. In the present invention, the "SNP" refers to an SNP registered in the SNP database (http: / / www.ncbi.nlm.nih.gov / SNP / ) of the National Center for Biotechnology Information (NCBI), which is a public database that is freely available to those skilled in the art, and can be identified by its reference number, the rs number.

[0015] As used herein, the term "allele" refers to each type having different bases that can be taken at a certain SNP site. In addition, as used herein, the term "genotype" refers to a combination of opposing alleles at a certain SNP site. At a certain SNP site, there are three types of genotypes that are the above combinations, and the combination of the same alleles is called a homozygous type, and the combination of different alleles is called a heterozygous type. For example, there are three types of genotypes that are combinations of opposing alleles at the SNP specified by rs557778 in (a1): A / A type, A / G type, and G / G type.

[0016] In the determination method of the present invention, "detecting an allele of a single nucleotide polymorphism (SNP)" means identifying the type of base of the allele of the SNP, and embodiments of "detecting an allele of a single nucleotide polymorphism (SNP)" include detecting one allele of the SNP, detecting both alleles of the SNP, and identifying the genotype of the SNP.

[0017] In the determination method of the present invention, when at least one of the bases of the detected allele is a risk allele, the subject is determined to have a genetic predisposition to rough skin (prone to rough skin). Specifically, when the risk allele shown in Table 3 below is detected in at least one allele in the single nucleotide polymorphisms (SNPs) (a1) to (a82), the subject can be determined to have a genetic predisposition to rough skin (prone to rough skin) compared to when the risk allele is not detected.

[0018] For example, in the case of the SNP identified by rs557778 in (a1), the genotype A / A indicates that the person is more likely to develop rough skin than the A / G or G / G types, and the genotype A / G indicates that the person is more likely to develop rough skin than the G / G type. In other words, the order of susceptibility to rough skin is A / A, A / G, and G / G.

[0019] [Table 3] TIFF0007629628000013.tif23974

[0020] In the method of the present invention, the race of the subject is not particularly limited, but is preferably East Asian, more preferably Japanese, where East Asian refers to a person originating from any of Japan, Korea, China, Taiwan, and Mongolia.

[0021] The DNA-containing sample used in the determination method of the present invention is not particularly limited as long as it is a biological sample containing DNA collected from a subject. The DNA contained in the DNA-containing sample is preferably genomic DNA, but when the SNP to be detected is an SNP located in a region present in mRNA other than a non-transcriptional region such as a promoter or a region removed by RNA splicing such as an intron, a biological sample containing mRNA or total RNA may be used instead of genomic DNA. As the DNA-containing sample, for example, any body fluid, secretion, tissue, cell, culture of tissue or cell, etc. from which genomic DNA can be collected can be used. Specifically, the subject's saliva, blood, urine, sputum, pharyngeal swab, nasal swab, oral (inner cheek) mucosa swab, lacrimal gland secretion, sweat, hair, nails, skin, mucous membrane, tape strip peeled off after skin attachment, etc. can be mentioned, but saliva is preferable in terms of ease and low invasiveness. The sample can be collected according to a method performed in a general clinical test and prepared using a known extraction method and purification method. In this case, a commercially available genomic DNA extraction kit can be used.

[0022] The method of detecting SNPs and determining the type of SNPs (SNP typing) is not particularly limited, and can be performed by known methods such as a method of amplifying by PCR or the like using allele-specific primers (and probes) and detecting the polymorphism of the amplified product by fluorescence or luminescence. Examples of such methods include PCR-RFLP (restriction fragment length polymorphism), PCR-SSCP (single-strand conformation polymorphism), PCR-SSO (sequence specific oligonucleotide), direct sequencing, ASO (Allele Specific Oligonucleotide) hybridization, ASP-PCR (Allele Specific Primer-PCR), Snapshot, ARMS (Amplification Refracting Mutation System), TaqMan PCR, Invader, MALDI-TOF / MS, RNase A cleavage, DOL (Dye-labeled Oligonucleotide Ligation), and TDI (Template-directed Dye-terminator Incorporation). All of the above methods are well known to those skilled in the art, and reagents and kits for determining SNP types are commercially available. For example, TaqMan SNP Genotyping Assays (Thermo Fisher Scientific) can be used.

[0023] The results obtained by the above-mentioned determination method are useful indicators for selecting cosmetics and / or foods and beverages that have the effect of preventing and / or improving rough skin for a subject, and for example, measures to prevent rough skin can be recommended to a subject who has been determined to have a genetic predisposition to rough skin (prone to rough skin). Therefore, according to another aspect of the present invention, there is also provided a method for providing cosmetics and / or foods and beverages that have the effect of preventing and / or improving rough skin to a subject based on the results obtained by the above-mentioned determination method, in which a cosmetics and / or foods and beverages that have the effect of preventing and / or improving rough skin are provided to the subject according to the degree of the subject's genetic predisposition to rough skin (proneness to rough skin).

[0024] 2. Kit for determining genetic predisposition to rough skin In the above-mentioned methods for detecting and typing SNPs, probes and primers appropriate for each method are used. Such probes and primers are also included in the scope of the present invention and can be provided as a kit.

[0025] The probe may include a probe that contains the above-mentioned SNP site and can distinguish the type of base at the SNP site based on the presence or absence of hybridization. Specifically, the probe may include a probe having a sequence of at least 10 consecutive bases, preferably 15 bases or more, including the 101st base of the base sequence shown in any one of SEQ ID NOs: 1 to 82, or a complementary sequence thereof. The length of the probe is preferably 15 to 40 bases, more preferably 20 to 35 bases. The probe may be labeled with an appropriate labeling substance, and examples of the labeling substance include enzymes (peroxidase, β-galactosidase, alkaline phosphatase, etc.), fluorescent substances (FITC, RITC, Cy3, Cy5, etc.), luminescent substances (luminol, luminol derivatives, luciferin, lucigenin, etc.), radioisotopes ( 3 H, 14 C. 32 P, 125 I, 131 I, etc.), biotin, digoxigenin, a polypeptide containing a tag sequence, etc. Alternatively, a quencher (quenching substance) that absorbs the fluorescent energy emitted by the fluorescent substance may be further bound to the vicinity of the fluorescent substance.

[0026] The probes may also be fixed to a solid phase (DNA array). In a DNA array, sample DNA is hybridized to a number of probes arranged on the same plane, and hybridization to each probe can be detected simultaneously by scanning the plane. Thus, a DNA array is useful for simultaneously analyzing a number of SNP sites. Oligonucleotides that serve as probes to be mounted on the array are usually synthesized in situ. For example, in situ synthesis methods of oligonucleotides using a lithography method (Thermo Fisher Scientific), an inkjet method (Agilent), a bead array method (Illumina), etc. are known.

[0027] Examples of the primer include a primer that can be used in PCR for amplifying the SNP site, or a primer that can be used for sequence analysis (sequencing) of the SNP site. Specifically, examples include a primer that can amplify or sequence a region containing the 101st base of the base sequence shown in any one of SEQ ID NOs: 1 to 82. The primer that can be used in PCR for amplifying the SNP site may be an oligonucleotide that can initiate complementary strand synthesis toward the SNP site using the DNA of the region containing the SNP site as a template, and the length of such a primer is preferably 10 to 30 bases, more preferably 15 to 25 bases. The primer can be set at a position upstream or downstream of the SNP site in the base sequence shown in any one of SEQ ID NOs: 1 to 82.

[0028] Those skilled in the art can design probes and primers according to the analysis method based on the base sequence information of the surrounding DNA region including the SNP site. In addition, oligonucleotides to be used as probes and primers can be synthesized by a method known in the art as a method for synthesizing oligonucleotides, such as the phosphoramidite method and the H-phosphonate method, using a commonly used automatic DNA synthesizer.

[0029] The kit of the present invention may contain at least the above-mentioned probe and oligonucleotides used as primers. The kit may also contain, as necessary, a DNA extraction reagent, a PCR buffer solution, a PCR reagent such as DNA polymerase, a detection reagent such as a staining agent or an electrophoresis gel, an immobilization carrier, a labeling substance, a substrate compound used to detect the label, positive and negative standard samples, and an instruction manual that describes how to use the kit. The reagents in the kit may be in the form of a solution or a lyophilized product.

[0030] 3. Screening method for agents for preventing and / or improving rough skin As described above, VAV3, RBM43, NMI, TNFAIP6, and RNF8 are genes that are known in the literature to have the SNPs (a1) to (a82) above in their vicinity (within 100 kb) or whose expression is altered by the SNPs.

[0031] Therefore, according to another aspect of the present invention, there is provided a method for screening for agents for preventing and / or improving rough skin, using changes in expression of one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8 (hereinafter referred to as "rough skin susceptibility genes") as an indicator.

[0032] The screening method of the present invention is not particularly limited as long as it is a method using cells suitable for evaluating the expression of rough skin susceptibility genes, but typically includes a method of measuring the expression level of rough skin susceptibility genes in cells. More specifically, the screening method of the present invention can be performed by carrying out the steps of culturing cells expressing the rough skin susceptibility genes in the presence of a test substance, measuring the expression level of the rough skin susceptibility genes in the cells, measuring the expression level of the rough skin susceptibility genes in the cells in the absence of the test substance (control), and selecting the test substance as a candidate substance for preventing and / or improving rough skin when the expression level measured in the presence of the test substance is significantly increased or decreased compared to the expression level measured in the absence of the test substance.

[0033] In the present invention, the expression level of the rough skin susceptibility gene in the test sample and the control sample can be measured by any method known to those skilled in the art, and the measurement may be performed according to the standard method for each method. The expression level of a gene refers to the amount of mRNA, which is the transcription product of the gene. The measurement of the amount of mRNA is not particularly limited as long as it can measure the desired amount of mRNA, and can be appropriately selected from known methods. For example, a gene amplification method using an oligonucleotide that hybridizes to the rough skin susceptibility gene as a primer, or a hybridization method using an oligonucleotide that hybridizes to the rough skin susceptibility gene as a probe can be used. Specifically, RT-PCR, real-time RT-PCR, microarray, northern blot, dot blot, RNase protection assay, etc. can be mentioned. The primers and probes used in the above methods are labeled, and the amount of mRNA can be measured by examining the signal intensity of the label. Among them, the real-time RT-PCR method is preferable because RNA can be used directly as a sample, and the gene amplification process can be optically measured to quantitatively determine the gene quantity from the number of temperature cycles required for amplification. The primers and probes used in the above method can be appropriately designed and prepared by a person skilled in the art based on the base sequence of the rough skin susceptibility gene obtained from a database such as GenBank. Various protocols have been reported for each method, and a person skilled in the art can carry out the method according to a known protocol or by appropriately modifying or changing the known protocol.

[0034] In another embodiment of the screening method of the present invention, a cell expressing a rough skin susceptibility gene may be used in which a plasmid in which a reporter gene is operably linked to the transcriptional regulatory region of the rough skin susceptibility gene has been introduced, and the expression level of the reporter gene may be measured. Examples of reporter genes include the GFP gene, the GUS gene, and the LUS gene.

[0035] The test substances used in screening are mainly components that can be used in pharmaceuticals and / or food and beverages, and include, for example, mixtures containing multiple compounds such as extracts from animal and plant tissues or microbial cultures, and preparations purified from them; naturally occurring molecules (e.g., amino acids, peptides, oligopeptides, polypeptides, proteins, nucleic acids, lipids, steroids, glycoproteins, proteoglycans, etc.); or synthetic analogs or derivatives of naturally occurring molecules (e.g., peptidomimetics, etc.); and non-naturally occurring molecules (e.g., small organic compounds produced using combinatorial chemistry techniques, etc.); as well as mixtures thereof. In addition, as a test substance, a single test substance may be tested independently, or a mixture (including a library, etc.) of several candidate test substances may be tested. Libraries containing multiple test substances include synthetic compound libraries and peptide libraries.

[0036] The substance for preventing and / or improving rough skin selected by the above screening method can be used as an ingredient in a composition for preventing and / or improving rough skin, such as cosmetics, medicines, quasi-drugs, foods and beverages, etc. Thus, according to yet another aspect of the present invention, there is provided a composition for preventing and / or improving rough skin, comprising a material that controls the expression of one or more genes selected from the group consisting of VAV3, RBM43, NMI, TNFAIP6, and RNF8. EXAMPLES

[0037] The present invention will be described in more detail below with reference to examples, although the present invention is not limited to these.

[0038] (Example 1) Identification of single nucleotide polymorphisms (SNPs) associated with genetic predisposition to rough skin (susceptibility to rough skin) To conduct this study, the consent form, genotype determination, etc. were approved by our company's ethical review committee. Samples were collected from sample donors who had given their consent by signing the consent form, and genotype determination and genome-wide association study (GWAS) were performed.

[0039] (1) DNA sample Saliva was collected from 1,206 female subjects, and genomic DNA was extracted using the Maxwell RSC Stabilized Saliva DNA Kit (Promega) to obtain DNA samples.

[0040] (2) Genotyping and quality control The DNA samples obtained were genotyped using Illumina's Asian Screening Array (ASA). As a result, approximately 650,000 SNPs were detected. Using the genetic statistical analysis software PLINK (www.cog-genomics.org / plink / 1.9 / ), subjects with a call rate of less than 0.95 and SNPs with a call rate of less than 0.95 were excluded from the 1,206 subjects and SNPs mentioned above. In addition, the p-value of the exact test of Hardy-Weinberg equilibrium was 1 × 10 -3 SNPs with a minor allele frequency (MAF) of less than 5% were also excluded. To remove unconfirmed relatedness from the genotype data, individual pairs with PI_HAT=0.25 or more were detected, and for each pair, individuals with low individual call rates were excluded from the analysis. In addition, clustering was performed using multidimensional scaling from the genotype data to select individuals of Japanese origin. The number of individuals selected for analysis through quality control was 1108, with 305180 SNPs.

[0041] (3) Genotype imputation Genotype imputation is a method for statistically estimating genotype information for sites not designed in the SNP array by referring to information from a standard collection of SNPs called a reference panel. In this example, genotype imputation was performed using Eagle and Minimac3 based on the 1000 Genome Project Phase 3 reference panel. After imputation, SNPs with a call rate of less than 0.95 and p-values ​​of the exact test of Hardy-Weinberg equilibrium were estimated to be 1 × 10 -3We excluded SNPs with a value of less than 0.3, SNPs with an Rsq value (an index that indicates the accuracy of imputation results) less than 0.3, and SNPs with a minor allele frequency (MAF) less than 5%, and ultimately imputed 6,258,233 SNPs.

[0042] (4) Phenotype data (susceptibility to skin irritation) The questionnaire item regarding the tendency for skin irritation was, "Do you tend to develop skin irritation due to stress, lack of sleep, or seasonal changes?", to which respondents were asked to answer one of four options: "1. I rarely have skin irritation," "2. I rarely have skin irritation," "3. I sometimes have skin irritation," or "4. I often have skin irritation," and was treated as an interval scale.

[0043] (5) Related Analysis As described above, regarding the correlation between the determined or estimated genotype data and the susceptibility to rough skin, a multiple regression analysis was performed using PLINK with the phenotype data as the objective variable, the number of minor alleles in the genotype data, and age as the explanatory variables, and the p-value of the regression coefficient of the number of minor alleles was evaluated. The p-value calculated by the multiple regression analysis was 1×10 -5 Eighty-two SNPs with a mean value of less than 1 were extracted as being associated with the phenotype.

[0044] For SNPs that were suggested to be associated with the susceptibility to rough skin, HaploReg v4.1 (https: / / pubs.broadinstitute.org / mammals / haploreg / haploreg.php) was used to annotate genes near the SNP (within 100 kb) and genes whose expression is known in the literature to change due to the SNP.

[0045] (6) Results From the above analysis, 82 SNPs were identified as SNPs that are associated with the genetic predisposition to rough skin and can determine the susceptibility to rough skin (Table 4). The SNP ID (NCBI dsSNP) of each identified SNP, the chromosome number and physical position where the SNP is located, the base of the allele and minor allele, the base of the risk allele, the effect size of the minor allele (β value), and the p-value of the genotype are shown. In the p-value column, each p-value is displayed in exponential form with the base 10, and the numbers written before and after the symbol E respectively indicate the mantissa and exponent when the p-value is displayed in exponential form. A positive β value means that the minor allele increases the risk (susceptibility to rough skin). Conversely, a negative β value means that the minor allele decreases the risk. "Imputed or Direct Typing" indicates whether each SNP was genotyped directly or imputed.

[0046] In addition, we found VAV3, RBM43, NMI, TNFAIP6, and RNF8 as genes located near the SNP (within 100 kb) (Table 4, gene (I)) and genes whose expression is known in the literature to be affected by the SNP (Table 4, gene (II)).

[0047] [Table 4] TIFF0007629628000015.tif243164TIFF0007629628000016.tif242164TIFF0007629628000017.tif24269 [Industrial Applicability]

[0048] The method of the present invention makes it possible to accurately and simply determine whether a sample donor has a genetic predisposition to rough skin. Based on the results of the determination, it is possible to provide the sample donor with customized cosmetics and supplements for preventing or improving rough skin, and to provide counseling and advice on care methods for improving rough skin.

Claims

1. A method for determining a genetic predisposition to rough skin, comprising the steps of: detecting alleles of one or more of the following single nucleotide polymorphisms (SNPs) (a1) to (a45) in a DNA-containing sample collected from a subject; and determining that the subject has a genetic predisposition to rough skin when at least one of the bases of the detected allele is a risk allele. (a1) SNP at the 101st base of the base sequence shown in SEQ ID NO: 1 (SNP: SNP identified by ID rs557778, risk allele is A) (a2) SNP at the 101st base of the base sequence shown in SEQ ID NO: 2 (SNP: SNP identified by ID rs555337, risk allele is G) (a3) SNP at the 101st base of the base sequence shown in SEQ ID NO: 3 (SNP: SNP identified by ID rs666003, risk allele is G) (a4) SNP at the 101st base of the base sequence shown in SEQ ID NO: 4 (SNP: SNP identified by ID rs666460, risk allele is G) (a5) SNP at the 101st base of the base sequence shown in SEQ ID NO: 5 (SNP: SNP identified by ID rs666461, risk allele is G) (a6) SNP at the 101st base of the base sequence shown in SEQ ID NO: 6 (SNP: SNP identified by ID rs526733, risk allele is A) (a7) SNP at the 101st base of the base sequence shown in SEQ ID NO: 7 (SNP: SNP identified by ID rs525842, risk allele is C) (a8) SNP at the 101st base of the base sequence shown in SEQ ID NO: 8 (SNP: SNP identified by ID rs4915051, risk allele is G) (a9) SNP at the 101st base of the base sequence shown in SEQ ID NO: 9 (SNP: SNP identified by ID rs680867, risk allele is T) (a10) SNP at the 101st base of the base sequence shown in SEQ ID NO: 10 (SNP: SNP identified by ID rs499513, risk allele is A) (a11) SNP at the 101st base of the base sequence shown in SEQ ID NO: 11 (SNP: SNP identified by ID rs684624, risk allele is A) (a12) SNP at the 101st base of the base sequence shown in SEQ ID NO: 12 (SNP: SNP identified by ID rs494757, risk allele is A) (a13) SNP at the 101st base of the base sequence shown in SEQ ID NO: 13 (SNP: SNP identified by ID rs494696, risk allele is C) (a14) SNP at the 101st base of the base sequence shown in SEQ ID NO: 14 (SNP: SNP identified by ID rs580995, risk allele is A) (a15) SNP at the 101st base of the base sequence shown in SEQ ID NO: 15 (SNP: SNP identified by ID rs515202, risk allele is A) (a16) SNP at the 101st base of the base sequence shown in SEQ ID NO: 16 (SNP: SNP identified by ID rs566923, risk allele is A) (a17) SNP at the 101st base of the base sequence shown in SEQ ID NO: 17 (SNP: SNP identified by ID rs560683, risk allele is C) (a18) SNP at the 101st base of the base sequence shown in SEQ ID NO: 18 (SNP: SNP identified by ID rs560655, risk allele is G) (a19) SNP at the 101st base of the base sequence shown in SEQ ID NO: 19 (SNP: SNP identified by ID rs663391, risk allele is T) (a20) SNP at the 101st base of the base sequence shown in SEQ ID NO: 20 (SNP: SNP identified by ID rs663431, risk allele is T) (a21) SNP at the 101st base of the base sequence shown in SEQ ID NO: 21 (SNP: SNP identified by ID rs12032660, risk allele is T) (a22) SNP at the 101st base of the base sequence shown in SEQ ID NO: 22 (SNP: SNP identified by ID rs12035053, risk allele is G) (a23) SNP at the 101st base of the base sequence shown in SEQ ID NO: 23 (SNP: SNP identified by ID rs11185132, risk allele is C) (a24) SNP at the 101st base of the base sequence shown in SEQ ID NO: 24 (SNP: SNP identified by ID rs6665028, risk allele is G) (a25) SNP at the 101st base of the base sequence shown in SEQ ID NO: 25 (SNP: SNP identified by ID rs6702592, risk allele is A) (a26) SNP at the 101st base of the base sequence shown in SEQ ID NO: 26 (SNP: SNP identified by ID rs534891, risk allele is T) (a27) SNP at the 101st base of the base sequence shown in SEQ ID NO: 27 (SNP: SNP identified by ID rs666070, risk allele is G) (a28) SNP at the 101st base of the base sequence shown in SEQ ID NO: 28 (SNP: SNP identified by ID rs666956, risk allele is T) (a29) SNP at the 101st base of the base sequence shown in SEQ ID NO: 29 (SNP: SNP identified by ID rs1752681, risk allele is A) (a30) SNP at the 101st base of the base sequence shown in SEQ ID NO: 30 (SNP: SNP identified by ID rs505822, risk allele is A) (a31) SNP at the 101st base of the base sequence shown in SEQ ID NO: 31 (SNP: SNP identified by ID rs504881, risk allele is A) (a32) SNP at the 101st base of the base sequence shown in SEQ ID NO: 32 (SNP: SNP identified by ID rs504806, risk allele is A) (a33) SNP at the 101st base of the base sequence shown in SEQ ID NO: 33 (SNP: SNP identified by ID rs501057, risk allele is A) (a34) SNP at the 101st base of the base sequence shown in SEQ ID NO: 34 (SNP: SNP identified by ID rs499227, risk allele is T) (a35) SNP at the 101st base of the base sequence shown in SEQ ID NO: 35 (SNP: SNP identified by ID rs586180, risk allele is C) (a36) SNP at the 101st base of the base sequence shown in SEQ ID NO: 36 (SNP: SNP identified by ID rs476157, risk allele is A) (a37) SNP at the 101st base of the base sequence shown in SEQ ID NO: 37 (SNP: SNP identified by ID rs475072, risk allele is C) (a38) SNP at the 101st base of the base sequence shown in SEQ ID NO: 38 (SNP: SNP identified by ID rs473420, risk allele is C) (a39) SNP at the 101st base of the base sequence shown in SEQ ID NO: 39 (SNP: SNP identified by ID rs472527, risk allele is C) (a40) SNP at the 101st base of the base sequence shown in SEQ ID NO: 40 (SNP: SNP identified by ID rs580075, risk allele is A) (a41) SNP at the 101st base of the base sequence shown in SEQ ID NO: 41 (SNP: SNP identified by ID rs549903, risk allele is A) (a42) SNP at the 101st base of the base sequence shown in SEQ ID NO: 42 (SNP: SNP identified by ID rs10785826, risk allele is C) (a43) SNP at the 101st base of the base sequence shown in SEQ ID NO: 43 (SNP: SNP identified by ID rs494152, risk allele is T) (a44) SNP at the 101st base of the base sequence shown in SEQ ID NO: 44 (SNP: SNP identified by ID rs643908, risk allele is C) (a45) SNP at the 101st base of the base sequence shown in SEQ ID NO: 45 (SNP: SNP identified by ID rs11811454, risk allele is C)

2. The method according to claim 1, further comprising the steps of detecting one or more single nucleotide polymorphism (SNP) alleles of (a46) to (a82) below, and determining that the subject has a genetic predisposition to rough skin if at least one of the bases of the detected allele is a risk allele. (a46) SNP at the 101st base of the base sequence shown in SEQ ID NO: 46 (SNP: SNP identified by ID rs61819207, risk allele is T) (a47) SNP at the 101st base of the base sequence shown in SEQ ID NO: 47 (SNP: SNP identified by ID rs289852, risk allele is C) (a48) SNP at the 101st base of the base sequence shown in SEQ ID NO: 48 (SNP: SNP identified by ID rs1056726, risk allele is A) (a49) SNP at the 101st base of the base sequence shown in SEQ ID NO: 49 (SNP: SNP identified by ID rs149819999, risk allele is G) (a50) SNP at the 101st base of the base sequence shown in SEQ ID NO: 50 (SNP: SNP identified by ID rs60254106, risk allele is C) (a51) SNP at the 101st base of the base sequence shown in SEQ ID NO: 51 (SNP: SNP identified by ID rs2173313, risk allele is T) (a52) SNP at the 101st base of the base sequence shown in SEQ ID NO: 52 (SNP: SNP identified by ID rs12209775, risk allele is C) (a53) SNP at the 101st base of the base sequence shown in SEQ ID NO: 53 (SNP: SNP identified by ID rs12211110, risk allele is A) (a54) SNP at the 101st base of the base sequence shown in SEQ ID NO: 54 (SNP: SNP identified by ID rs7738045, risk allele is G) (a55) SNP at the 101st base of the base sequence shown in SEQ ID NO: 55 (SNP: SNP identified by ID rs1937834, risk allele is T) (a56) SNP at the 101st base of the base sequence shown in SEQ ID NO: 56 (SNP: SNP identified by ID rs75741813, risk allele is T) (a57) SNP at the 101st base of the base sequence shown in SEQ ID NO: 57 (SNP: SNP identified by ID rs74686576, risk allele is G) (a58) SNP at the 101st base of the base sequence shown in SEQ ID NO: 58 (SNP: SNP identified by ID rs74415127, risk allele is A) (a59) SNP at the 101st base of the base sequence shown in SEQ ID NO: 59 (SNP: SNP identified by ID rs77750385, risk allele is G) (a60) SNP at the 101st base of the base sequence shown in SEQ ID NO: 60 (SNP: SNP identified by ID rs76145983, risk allele is A) (a61) SNP at the 101st base of the base sequence shown in SEQ ID NO: 61 (SNP: SNP identified by ID rs75155123, risk allele is G) (a62) SNP at the 101st base of the base sequence shown in SEQ ID NO: 62 (SNP: SNP identified by ID rs74302809, risk allele is T) (a63) SNP at the 101st base of the base sequence shown in SEQ ID NO: 63 (SNP: SNP identified by ID rs111523535, risk allele is T) (a64) SNP at the 101st base of the base sequence shown in SEQ ID NO: 64 (SNP: SNP identified by ID rs199627764, risk allele is A) (a65) SNP at the 101st base of the base sequence shown in SEQ ID NO: 65 (SNP: SNP identified by ID rs79187933, risk allele is G) (a66) SNP at the 101st base of the base sequence shown in SEQ ID NO: 66 (SNP: SNP identified by ID rs77784037, risk allele is A) (a67) SNP at the 101st base of the base sequence shown in SEQ ID NO: 67 (SNP: SNP identified by ID rs78530814, risk allele is C) (a68) SNP at the 101st base of the base sequence shown in SEQ ID NO: 68 (SNP: SNP identified by ID rs4739577, risk allele is T) (a69) SNP at the 101st base of the base sequence shown in SEQ ID NO: 69 (SNP: SNP identified by ID rs78484770, risk allele is T) (a70) SNP at the 101st base of the base sequence shown in SEQ ID NO: 70 (SNP: SNP identified by ID rs76611879, risk allele is T) (a71) SNP at the 101st base of the base sequence shown in SEQ ID NO: 71 (SNP: SNP identified by ID rs80335309, risk allele is C) (a72) SNP at the 101st base of the base sequence shown in SEQ ID NO: 72 (SNP: SNP identified by ID rs75558330, risk allele is C) (a73) SNP at the 101st base of the base sequence shown in SEQ ID NO: 73 (SNP: SNP identified by ID rs76151608, risk allele is G) (a74) SNP at the 101st base of the base sequence shown in SEQ ID NO: 74 (SNP: SNP identified by ID rs74407439, risk allele is A) (a75) SNP at the 101st base of the base sequence shown in SEQ ID NO: 75 (SNP: SNP identified by ID rs112276204, risk allele is G) (a76) SNP at the 101st base of the base sequence shown in SEQ ID NO: 76 (SNP: SNP identified by ID rs75023163, risk allele is G) (a77) SNP at the 101st base of the base sequence shown in SEQ ID NO: 77 (SNP: SNP identified by ID rs4739578, risk allele is C) (a78) SNP at the 101st base of the base sequence shown in SEQ ID NO: 78 (SNP: SNP identified by ID rs4739579, risk allele is G) (a79) SNP at the 101st base of the base sequence shown in SEQ ID NO: 79 (SNP: SNP identified by ID rs16882394, risk allele is G) (a80) SNP at the 101st base of the base sequence shown in SEQ ID NO: 80 (SNP: SNP identified by ID rs77736544, risk allele is C) (a81) SNP at the 101st base of the base sequence shown in SEQ ID NO: 81 (SNP: SNP identified by ID rs78585145, risk allele is C) (a82) SNP at the 101st base of the base sequence shown in SEQ ID NO: 82 (SNP: SNP identified by ID rs16882399, risk allele is A)

3. A method for providing cosmetics and / or food and beverages, comprising providing a subject with cosmetics and / or food and beverages having the effect of preventing and / or improving rough skin according to the degree of the subject's genetic predisposition to rough skin based on the results determined by the method described in claim 1 or 2.

4. A kit for determining a genetic predisposition to rough skin, comprising a probe having a sequence of 10 or more bases including the 101st base in a base sequence shown in any one of SEQ ID NOs: 1 to 45, or a complementary sequence thereof, and / or a primer capable of amplifying a region including the 101st base in a base sequence shown in any one of SEQ ID NOs: 1 to 45.

5. The kit described in claim 4, further comprising a probe having a sequence of 10 or more bases including the 101st base in a base sequence shown in any of SEQ ID NOs: 46 to 82, or a complementary sequence thereof, and / or a primer capable of amplifying a region including the 101st base in a base sequence shown in any of SEQ ID NOs: 46 to 82.

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