Sampling device and sampling method

Abstract

The embodiment of the present invention provides a sample cutter head unit, a crushing device, and a sampling device, which are related to the technical field of biological detection. The sample cutter head unit includes a cutter head body and a second blade. However, a first blade is formed at one end of the cutter head body in the circumferential direction of the cutter head body. The second blade is connected to the cutter head body and is located at the same end of the cutter head body together with the first blade, and at least a part of the second blade is located inside the first blade, and is provided to surround a plurality of sub-sample areas together with the first blade. The sample cutter head unit of the embodiment of the present invention achieves crushing and cutting of plant leaves and petals through cooperation with the second blade and the first blade, improves the sampling efficiency of plant leaves and petals, and promotes plant extraction research activities.

Description

[Technical field]

[0001] The present invention relates to the technical field of biological detection, and in particular to a head unit of a sample cutter, a crushing device and a sampling device. [Background technology]

[0002] As the importance of biological detection technology increases, there is an urgent need to extract plant DNA. The cutters in related technologies cannot efficiently cut and sample plants, which affects the efficiency of plant DNA extraction. Summary of the Invention [Problem to be solved by the invention]

[0003] In view of this, an object of the embodiments of the present invention is to provide a sample cutter head unit, a crushing device, and a sampling device that are easy to sample in order to crush a sampled sample. [Means for solving the problem]

[0004] An embodiment of the present invention is a head unit of a sample cutter, A cutter head body having a first blade provided in a circumferential direction at one end thereof;

[0005] The present invention provides a sample cutter head unit, comprising: a second blade connected to the cutter head body, positioned at the same end of the cutter head body as the first blade, at least a portion of which is positioned inside the first blade, and which, together with the first blade, surrounds a plurality of sub-sample areas.

[0006] In some embodiments, the cutterhead body defines a hollow chamber, the second blade is disposed partially within the chamber, and the sub-sample region is in communication with the chamber. In some embodiments, the first blade is annular in shape. In some embodiments, the second blades are arranged in a circumferential direction of the cutterhead body and are connected to each other at their inner sides.

[0007] In some embodiments, a direction in which the second blade moves toward the cutterhead body along an axial direction of the cutterhead body is a predetermined direction, a distance between the edge of the second blade and the edge of the first blade along a radial direction of the cutterhead body is a target distance, the target distance is gradually decreased along the predetermined direction, and a plurality of the second blades cross to form cutting edges on the inside of the second blades.

[0008] In some embodiments, the second blade has a contact portion connected to the cutterhead body and a spacing portion located between the first blade and the contact portion along the axial direction of the cutterhead body, and the spacing portion and the first blade are spaced apart along the radial direction of the cutterhead body.

[0009] In some embodiments, the second blades have a thickness that gradually decreases from the inside to the outside.

[0010] An embodiment of the present invention is a crushing device, A head unit of the sample cutter described in the above embodiment; The present invention further provides a crushing device comprising: a support assembly formed with a sample passage and a support surface for placing the sample object, an opening of the sample passage being positioned on the support surface, and a head unit of the sample cutter being movable into the sample passage through the opening of the sample passage.

[0011] In some embodiments, the support assembly comprises: a support plate having the support surface formed thereon and penetrated by the sample passage; a storage box in which the sealed end of the sample passage is located.

[0012] An embodiment of the present invention is a sampling device, comprising: The sampling body, A crushing device according to claim 1 attached to the sampling body; The present invention further provides a sampling device comprising: a driving device attached to the sampling body for driving and moving a head unit of the sample cutter. Effect of the Invention

[0013] The head unit of the sample cutter according to the embodiment of the present invention, in cooperation with the second blade and the first blade, achieves crushing and cutting of plant leaves and petals, improving the sampling efficiency of plant leaves and petals, and facilitating research activities on plant DNA extraction. [Brief description of the drawings]

[0014] [Figure 1] FIG. 1 is a structural schematic diagram of a head unit of a sample cutter according to an embodiment of the present invention. [Diagram 2] FIG. 2 is a schematic cross-sectional view of a head unit of the sample cutter in FIG. [Diagram 3] FIG. 3 is a partially enlarged schematic view of a portion A in FIG. [Figure 4] FIG. 4 is a structural schematic diagram of a sampling device according to an embodiment of the present invention. [Diagram 5] FIG. 5 is a structural schematic diagram of the trigger device and the storage box in FIG. [Figure 6] FIG. 6 is a plan view of the support assembly in FIG. [Figure 7] FIG. 7 is a cross-sectional view taken along the direction BB in FIG. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] In addition, unless otherwise specified, the examples and technical features in the examples of the present invention can be combined with each other, and the detailed description of the specific embodiments should be understood as an interpretation and explanation of the meaning of the present invention and do not unduly limit the present invention.

[0016] In the description of the embodiments of the present invention, the terms "upper", "lower", "top", and "bottom", orientations, and positional relationships are based on the orientations and positional relationships shown in Fig. 1. Note that these orientation terms are merely used to facilitate and simplify the description of the present invention, and do not explicitly or implicitly indicate that the devices or elements shown must have a specific orientation, or that they must be constructed or operated in a specific orientation, and therefore should not be understood as limitations on the present invention.

[0017] An embodiment of the present invention provides a sampling device. Referring to Fig. 4 to Fig. 7, the sampling device includes a sampling body 100, a driving device 300, and a crushing device 200. The crushing device 200 is attached to the sampling body 100. The driving device 300 is attached to the sampling body 100 and drives the crushing device 200 to crush the sample to be sampled.

[0018] In this embodiment, the sampling body 100 provides a berth for the sampling device and is used to attach the crushing device 200 and the driving device 300 to the sampling body 100. The driving device 300 is a power source for operating the crushing device 200, and is used to crush the sample to be sampled by operating and driving the crushing device 200.

[0019] The sampling device may be one for crushing and separating leaves and petals. The sampling device is not limited to sampling leaves and petals, and may be applied to other sampling targets.

[0020] 4 to 7, in one embodiment, the crushing device 200 includes a support assembly 20 and a sample cutter head unit 10. The support assembly 20 is formed with a sample passage and a support surface 23 for placing a sampling target. An opening of the sample passage is positioned on the support surface 23, and the sample cutter head unit 10 can be inserted into the sample passage through the opening of the sample passage.

[0021] In this embodiment, the support surface 23 is used as a positioning reference for the sample object for positioning the sample object. The head unit 10 of the sample cutter enters the sample passage through the opening of the sample passage to complete the separation of the sample object, and facilitates subsequent direct sampling by an operator from the sample passage. For example, the sample object is a leaf.

[0022] In one embodiment, the driving device 300 is for driving and moving the head unit 10 of the sample cutter. In this manner, the driving device 300 drives and moves the head unit 10 of the sample cutter to perform sampling on the sample object.

[0023] In one embodiment, the drive device 300 is for driving the head unit 10 of the sample cutter into the sample passage through the opening of the sample passage.

[0024] The relationship between the driving device 300 and the head unit 10 of the sample cutter is not limited. In some embodiments, the driving device 300 is directly connected to the head unit 10 of the sample cutter and drives the head unit 10 of the sample cutter to move. In other embodiments, the driving device 300 is separated from the head unit 10 of the sample cutter and drives the head unit 10 of the sample cutter to move using other media such as gunpowder or compressed gas. Since the driving device 300 and the head unit 10 of the sample cutter are separated, the cutter shape after sampling enters the sample passage through the opening of the sample passage along with the sampled sample.

[0025] The configuration of the support assembly 20 is not limited. In one embodiment, referring to Figs. 4 to 7, the support assembly 20 includes a support plate 20 and a storage box 24. However, the support surface 23 is formed in the support plate 20, and the sample passage passes through the support plate 20. The sealed end of the sample passage is located in the storage box 24.

[0026] Although the shape of the sample passage is not limited, in one embodiment, the sample passage is a blind hole, the opening of which is for passing the head unit 10 of the sample cutter and the bottom of which is for storing the head unit 10 of the sample cutter.

[0027] 4 to 7, in another embodiment, the sample passage is a sampling chamber and a storage chamber 25 that are connected to each other. However, in the sample passage, the sampling chamber is formed in the support plate 21, and the storage chamber 25 is formed in the storage box 24. That is, the head unit 10 of the sample cutter enters the storage chamber 25 through the sampling chamber provided in the support plate 21, and then the worker extracts the separated leaf or petal tissue from the storage chamber 25.

[0028] Although the material of the storage box 24 is not limited, in some embodiments, the storage box 24 adopts a PCR plate. The PCR plate is a carrier mainly involved in the amplification reaction in the polymerase chain reaction.

[0029] In some embodiments, the container 24 may be a sampling orifice plate, for example a 96 sampling orifice plate, a 48 sampling orifice plate, or the like.

[0030] The number of storage chambers 25 in the storage box 24 is not limited and may be created according to actual needs. However, the current storage chamber 25 for storing the head unit 10 of the sample cutter needs to be moved to a station below the opening of the sample passage, thereby forming a sample passage together with the opening of the sample passage.

[0031] An embodiment of the present invention provides a sample cutter head unit 10. Referring to Figs. 1 to 3, the sample cutter head unit 10 includes a sample cutter head unit body 1 and a second blade 3. A first blade 2 is formed at one end of the cutter head body 1 and arranged in the circumferential direction of the cutter head body 1. The second blade 3 is connected to the cutter head body 1 and is located at the same end of the cutter head body 1 together with the first blade 2, with at least a portion of the second blade 3 positioned inside the first blade 2, and is arranged to surround a plurality of sub-sample regions 4 together with the first blade 2.

[0032] The head unit 10 of the sample cutter may be for sampling leaves or petals of a plant, but is not limited to sampling leaves or petals of a plant.

[0033] The first blade 2 and the second blade 3 are arranged to surround a plurality of sub-sample regions 4, so that the leaf or petal sample sampled by the head unit 10 of the sample cutter is divided into sub-samples corresponding to the sub-sample regions 4 by the first blade 2 and the second blade 3 in cooperation, and there are a plurality of sub-sample regions 4, and the number of corresponding sub-samples is a plurality, that is, the leaf or petal sample sampled by the head unit 10 of the sample cutter is crushed into a plurality of sub-samples. The sample sample is crushed by cutting by the head unit 10 of the sample cutter and the first blade 2 and the second blade 3.

[0034] In one embodiment, the number of the second blades 3 is not limited, but may be, for example, three, four, or more. The specific number may be selected according to the specific demand for the crushing degree of the leaves or petals of the plant. In one embodiment, the number of second blades 3 may be one.

[0035] The first blades 2 are provided in the circumferential direction of the cutter head body 1 and are therefore useful for separating a sample from a sample target, for example, a leaf sample from a sample target.

[0036] The shape of the cutter head body 1 is not limited. In one embodiment, the shape of the cutter head body 1 may be cylindrical. In this way, the cylindrical cutter head body 1 is useful for mounting the head unit 10 of the sample cutter. In one embodiment, the shape of the cutter head body 1 may be a rectangular parallelepiped.

[0037] The head unit 10 of the sample cutter according to the embodiment of the present invention, in cooperation with the second blade 3 and the first blade 2, crushes and cuts the leaves and petals of plants, improving the sampling efficiency of the leaves and petals of plants and facilitating research activities on plant DNA extraction.

[0038] In one embodiment, referring to FIG. 1, a hollow chamber 1a is formed in a cutter head body 1, a second blade 3 is partially disposed within the chamber 1a, and a sub-sample region 4 is connected to the chamber 1a.

[0039] It can be seen that the head unit 10 of the sample cutter falls into the storage chamber 25 of the storage box 24 after sampling, and only a relatively narrow space can be formed between the head unit 10 of the sample cutter and the storage chamber 25, which makes it inconvenient for the operator to perform the sampling operation later. The chamber 1a in the cutter head body 1 provides the operator with a space for sampling, that is, the operator can perform the sampling operation through the chamber 1a in the cutter head body 1.

[0040] In this embodiment, some of the leaves or petals crushed by the second blade 3 enter the chamber 1a via the corresponding sub-sample area 4, which may be for depositing the sampled leaves or petals. The location of the chamber 1a in the cutter head body 1 is useful for subsequent sampling by the operator 10, and also for reducing the weight and saving materials of the sample cutter head unit 10. The shape of the first blade 2 is not limited.

[0041] In one embodiment, and referring to FIG. 1, the first blade 2 is annular in shape. In this embodiment, the annular arrangement of the first blade 2 can form a sealed region of leaves or petals. The first blade 2 separates the leaves or petals of the crushed plant by forming an edge of the sealed region. The separated leaves or petals correspond to the sub-sample region 4.

[0042] In one embodiment, the number of the first blades 2 is plural, and the first blades 2 are provided at intervals in the circumferential direction of the cutter head body 1 . In one embodiment, both ends of the first blade 2 along the circumferential direction of the cutter head body 1 are connected to two adjacent second blades 3, respectively.

[0043] In one embodiment, referring to Figs. 1 and 2, a plurality of second blades 3 are provided in the circumferential direction of the cutter head body 1, and the inner sides of the plurality of second blades 3 are connected to each other.

[0044] In addition, the direction toward the axis b of the cutter head body 1 along the radial direction of the cutter head body 1 is defined as the inward direction, and the direction away from the axis b along the radial direction of the cutter head body 1 is defined as the outward direction.

[0045] In this embodiment, the inner interconnection of multiple second blades 3 helps to improve the strength of the second blades 3, and the arrangement of the second blades 3 along the circumferential direction of the cutterhead body 1 helps to ensure uniform breaking of leaves and petals.

[0046] In one embodiment, referring to Figures 1 and 2, the direction in which the second blade 3 moves toward the cutter head body 1 along the axial direction of the cutter head body 1 is set to a predetermined direction (the predetermined direction is the direction indicated by the arrow in Figure 2), the distance between the edge 3a of the second blade and the edge 2a of the first blade along the radial direction of the cutter head body 1 is set to a target distance, and the target distance is gradually reduced along the predetermined direction, and the multiple second blades 3 cross each other to form cutting edges 5 on the inside of the second blades 3.

[0047] In this embodiment, it is ensured that the edge of the second blade 3a gradually converges to a sharp point along the direction away from the edge of the first blade 2a to form a cutting edge 5. The cutting edge 5 serves to pierce the local tissue of the leaf or petal and to break the leaf or petal by the head unit 10 of the sample cutter.

[0048] In one embodiment, referring to Figures 2 and 3, the second blade 3 has a contact portion 6 connected to the cutterhead body 1 and a spacing portion 7 positioned between the first blade 2 and the contact portion 6 along the axial direction of the cutterhead body 1, and the spacing portion 7 and the first blade 2 are spaced apart along the radial direction of the cutterhead body 1.

[0049] In this embodiment, the edge 2a of the first blade is formed completely and uninterrupted, thereby aiding the head unit 10 of the sample cutter in separating the entire leaf or petal.

[0050] In one embodiment, referring to FIG. 1, the second blade 3 has a thickness that gradually decreases from the inside to the outside. A straight line passing through the cutting edge 5 of the second blade 3 and along the axis of the cutter head body 1 overlaps with the axial line b, and if the side closer to the straight line is the inside of the second blade 3 and the side away from the straight line is the outside of the second blade 3, the thickness of the multiple second blades 3 gradually decreases from the inside to the outside.

[0051] In this embodiment, making the inside of the second blade 3 thicker helps to ensure the overall strength of the second blade 3, while making the outside of the second blade 3 thinner helps to form a sharp edge for cutting leaves and petals. The embodiments / exemplary aspects of the present invention may be combined with each other if not inconsistent.

[0052] The above is merely a preferred embodiment of the present invention, and is not intended to limit the present invention. Those skilled in the art may make various modifications and changes to the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention are all included in the scope of the protection claims of the present invention. [Explanation of symbols]

[0053] 1 Cutter head 1a Chamber 2 1st Blade 2 2a First blade edge 3. Second Blade 3a Second blade edge 4 Subsample Area 5 Cutting Edge b Axial center line 6 Contact Part 7 Spacing 10 Sample cutter head unit 20 Support assembly 21 Support plate 22 Sampling Chamber 23 Support surface 24 Storage Box 25 Storage Chamber 100 Sampling unit 200 Crushing Equipment 300 Drive Unit

Claims

1. A sample cutter head unit (10) comprising a cutter head body (1) and a second blade (3), A first blade (2) is formed at one end of the cutter head body (1), and the first blade (2) is provided in the circumferential direction of the cutter head body (1); The second blade (3) is connected to the cutterhead body (1) and is located at the same end of the cutterhead body (1) as the first blade (2); At least a portion of the second blade (3) is located inside the first blade (2); The first blade (2) and the second blade (3) are arranged together to surround a plurality of sub-sample regions (4). A sample cutter head unit (10).

2. The cutter head body (1) is formed with a hollow chamber (1a), The second blade (3) is partially provided within the chamber (1a), and the sub-sample region (4) is in communication with the chamber (1a). A sample cutter head unit (10) according to claim 1.

3. The first blade (2) is annular in shape. A sample cutter head unit (10) according to claim 1.

4. The second blades (3) are provided in the circumferential direction of the cutter head body (1), and the inner sides of the second blades (3) are connected to each other. A sample cutter head unit (10) according to claim 1.

5. A direction in which the second blade (3) faces the cutter head body (1) along the axial direction of the cutter head body (1) is a predetermined direction; a distance between the edge (3a) of the second blade (3) and the edge (2a) of the first blade along the radial direction of the cutter head body (1) is set as a target distance; The target distance is gradually decreased along the predetermined direction; The second blades (3) intersect to form a cutting edge (5) on the inside of the second blades (3). A sample cutter head unit (10) according to claim 2.

6. The second blade (3) is A contact portion (6) connected to the cutter head body (1); a spacing portion (7) located between the first blade (2) and the contact portion (6) along the axial direction of the cutter head body (1); The spacing portion (7) and the first blade (2) are spaced apart from each other along the radial direction of the cutter head body (1). A sample cutter head unit (10) according to claim 5.

7. The thickness of the second blades (3) gradually decreases from the inside to the outside. A sample cutter head unit (10) according to claim 5.

8. A crushing device (200), A sample cutter head unit (10) according to any one of claims 1 to 7; a support assembly (20) in which a sample passage and a support surface (23) for placing a sample object are formed, an opening of the sample passage is positioned on the support surface (23), and a head unit (10) of the sample cutter is movable into the sample passage through the opening of the sample passage; Equipped with A crushing device (200).

9. The support assembly (20) comprises: a support plate (21) on which the support surface (23) is formed and which is pierced by the sample passage; a storage box (24) in which the sealed end of the sample passage is located; Equipped with Crushing device (200) according to claim 8.

10. 1. A sampling device comprising: A sampling body (100); A crushing device (200) according to claim 8 or 9, which is attached to the sampling body (100); A driving device attached to the sampling body (100) for driving and moving the head unit (10) of the sample cutter; Equipped with A sampling device characterized by:

Images