Method for producing carotenoid pigment solution

Abstract

To provide a method for producing a carotenoid pigment solution, which affords a carotenoid pigment solution having strong redness with a yellow tinge suppressed.SOLUTION: Provided is a method for producing a carotenoid pigment solution, comprising making lipase act on an oily liquid containing a carotenoid pigment in the presence of a thickening polysaccharide to obtain a carotenoid pigment solution with strong redness. Further, a carotenoid pigment solution with stronger redness can be obtained by including a step of mixing calcium chloride. A carotenoid pigment solution prepared by the method for producing a carotenoid pigment solution has a color valency (10% E) of 20-2,000.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a method for producing a carotenoid pigment liquid, and more specifically, to a method for producing a carotenoid pigment liquid in which a carotenoid pigment-containing pigment and an oil or fat are mixed together and lipase is allowed to act on the carotenoid pigment liquid in the presence of a thickening polysaccharide to obtain a carotenoid pigment liquid with a strong reddish color and reduced yellowness. [Background technology]

[0002] Carotenoid pigments are one of the pigments used to give various colors to foods. Carotenoid pigments are present as reddish pigment components in tomatoes and peppers, but are generally used as yellow or orange colorings. Carotenoid pigments are fat-soluble and can be used dissolved in oil (oleoresin) or dispersed in water (emulsified). Reddish hues are preferred for foods such as crab sticks and spicy foods.

[0003] For example, Patent Document 1 discloses a method for obtaining a reddish carotenoid pigment by purifying an oily raw material containing carotenoids such as tomato pigment and β-carotene, crystallizing the crystals, and mixing them with a hydrophilic substance and pulverizing them. Patent Document 2 discloses a method for separating vivid red, yellow, and orange pigments from a paprika pigment obtained by treating a paprika pigment raw material with lipase. Meanwhile, Patent Document 3 discloses lipase treatment of carotenoid pigments by adding lipase to carotenoid-containing oleoresin, hydrolyzing the product, and distilling the resulting product. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent No. 6100227 [Patent Document 2] Special Publication No. 60-30515 [Patent Document 3] Special Publication No. 1-19826 Summary of the Invention [Problem to be solved by the invention]

[0005] It is known that when carotenoids are used as pigments and oils are mixed with the carotenoids, the carotenoids dissolve in the oils and exhibit a yellow-orange hue rather than the inherent red hue of carotenoids. Therefore, Patent Document 1 calls for the use of highly purified carotenoid raw materials or for the finer carotenoid raw materials to be subjected to advanced mechanical grinding. Patent Document 2 also describes a method in which a paprika pigment raw material is lipase-treated to obtain a processed product, which is dissolved in an organic solvent selected from paraffin-based hydrocarbons, and the resulting solution is washed with an aqueous alcohol solution or an aqueous potassium hydroxide-alcohol solution. The resulting purified paprika pigment concentrate is then subjected to silica gel chromatography to separate and obtain red, yellow, and orange pigments. However, the resulting color of the pigment when used in food is unknown, and the process requires a long stirring time of 4 hours at room temperature.

[0006] Furthermore, Patent Document 3 was made for the purpose of industrially advantageously producing a stable, high-purity, odorless carotenoid pigment, and is characterized in that, when purifying a carotenoid-containing oleoresin by molecular distillation, lipase is added to the oleoresin, and the hydrolyzed product is distilled. However, it is understood that the lipase in Patent Document 3 is used for purifying raw materials, such as removing odors and separating components.

[0007] Thus, conventionally, there has been a problem in that obtaining a carotenoid pigment with a strong reddish color requires a lot of time and effort.

[0008] Therefore, the present inventors have conducted extensive research to solve the above problems and have found that a carotenoid pigment solution with a strong reddish hue and reduced yellowness can be obtained by adding and mixing an enzyme, lipase, in the coexistence of a thickening polysaccharide to a carotenoid pigment mixed with fats and oils (oleoresin).Further research has led to the completion of the present invention. [Means for solving the problem]

[0009] In order to solve the above problem, the present invention as set forth in claim 1 provides: Carotenoids are dissolved in fats and oils The present invention provides a method for producing a carotenoid pigment liquid, characterized in that a carotenoid pigment liquid having a strong reddish color is obtained by allowing lipase to act on an oily liquid in the presence of a thickening polysaccharide.

[0010] In order to solve the above problems, the present invention as set forth in claim 2 provides a method for producing a carotenoid pigment solution as set forth in claim 1, comprising the steps of: adding the thickening polysaccharide to heated water to prepare a thickening polysaccharide aqueous solution; The oily liquid and adding a heated aqueous solution of thickening polysaccharides to the heated aqueous solution of thickening polysaccharides. The oily liquid and a step of treating the carotenoid mixed liquid with lipase.

[0011] In order to solve the above problem, the present invention as set forth in claim 3 is 2 In the method for producing a carotenoid pigment liquid described in the above item 1, the concentration of the thickening polysaccharide is 5.0 to 30.0% by mass, The oily liquid The color value (10% E) of the carotenoid is 200 to 6,500, and the concentration of the carotenoid in the carotenoid mixed liquid is 10.0 to 30.0% by mass.

[0012] In order to solve the above problem, the present invention as set forth in claim 4 is 2 or 3 The method for producing a carotenoid pigment liquid described in 1) above is characterized in that calcium chloride is further added in the step of preparing the thickening polysaccharide aqueous solution. [Effects of the Invention]

[0013] According to the method for producing a carotenoid pigment solution of the present invention, Carotenoids are dissolved in fats and oils When lipase is applied to an oily liquid, the presence of a thickening polysaccharide promotes the enzymatic reaction of the lipase, thereby shortening the treatment time. Furthermore, the presence of calcium chloride during the enzymatic treatment has the effect of producing a carotenoid pigment liquid with a more reddish color.

[0014] Furthermore, the carotenoid pigment solution produced by the present invention can be dispersed in water, making it easy to use in various foods. Furthermore, the carotenoid pigment solution obtained by the present invention has a concentration (color value) suitable for use in general food industry applications. [Brief explanation of the drawings]

[0015] [Figure 1] FIG. 1 is a flow chart showing one embodiment of the method for producing a carotenoid pigment solution according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0016] The method for producing a carotenoid pigment solution according to the present invention will be described in detail below based on preferred examples. Carotenoids are dissolved in fats and oils The method is characterized in that a carotenoid pigment liquid with a strong reddish color is obtained by treating an oily liquid with lipase in the presence of a thickening polysaccharide. First, each of these components will be described.

[0017] [Carotenoids] Carotenoid pigments are oily components that belong to the carotenoid family, and examples include the following. Note that the main components and their origins are listed in parentheses: annatto pigment (bixin, norbixin: the seed coating of the annatto tree (Bixaceae)), gardenia yellow pigment (crocin, crocetin: the fruit of gardenia (Rubiaceae)), Dunaliella carotene (carotenoid: the whole algae of Dunaliella (Volcanaceae)), carrot carotene (carotenoid: the dried root of carrot (Apiaceae)), palm oil carotene (carotenoid: palm oil obtained from the fruit of the oil palm (Arecaceae)), tomato pigment (lycopene: the fruit of tomato (Solanaceae)), chili pepper pigment (capsanthins: the fruit of chili pepper (Solanaceae)), marigold pigment (xanthophyll: the flower of marigold (Asteraceae)), and Haematococcus algae pigment (astaxanthin: algae). Other examples include β-carotene. The carotenoid in the present invention is preferably a pigment (oily component) belonging to xanthophylls, and is preferably a capsicum pigment, a marigold pigment, or a Haematococcus algae pigment. Carotenoids are dissolved in fats and oils Oily liquid (below," Carotenoid oleoresin " (also called ") The fats and oils constituting the food product are, for example, common edible fats and oils such as rapeseed oil, rice bran oil, medium-chain fatty acid triglyceride, etc. Note that these are only examples, and other edible fats and oils may also be included.

[0018] [Thickening polysaccharides] Thickening polysaccharides are generally water-soluble polymeric substances that exhibit viscosity or gelation when dissolved in water. Suitable thickening polysaccharides include those derived from sap, such as gum arabic, gum ghatti, karaya gum, tragacanth gum, locust bean gum, tara gum, guar gum, and tamarind seed gum. One or more of these sap-derived thickening polysaccharides can be used. Among these, gum arabic or gum ghatti are preferred due to their viscosity characteristics, and it is recommended to use at least one of gum arabic or gum ghatti. Other usable thickening polysaccharides include carrageenan, xanthan gum, and glucomannan. Decomposition products of the above-mentioned substances may also be used.

[0019] [Lipase] Lipase is a lipid-degrading enzyme that primarily degrades the ester bonds between glycerin and fatty acids. In the present invention, lipase efficiently degrades carotenoids dissolved in fats and oils in the presence of a thickening polysaccharide, causing the carotenoids to change from a dissolved state (yellow to orange) to an insoluble state (red). Furthermore, the thickening polysaccharides disperse the carotenoids in water, resulting in a more intensely red color. Any suitable lipase can be used; for example, Amano Enzyme's Lipase AY "Amano" 30SD (fat digestion capacity: 30,000 u / g) can be used. The fat digestion capacity was determined by neutralization titration with aqueous sodium hydroxide solution to determine the amount of fatty acid that increases with the cleavage of the ester bonds between fatty acids and glycerol when lipase is applied to olive oil.

[0020] [Calcium chloride] Calcium chloride is a calcium chloride with the chemical formula "CaCl2" and is a white, deliquescent crystal. Calcium chloride is a well-known substance, so a detailed description will be omitted.

[0021] Next, a preferred embodiment of the method for producing a carotenoid pigment solution according to the present invention will be described. Fig. 1 is a flowchart showing one embodiment of the method for producing a carotenoid pigment solution according to the present invention. Unless otherwise specified, "parts" means "parts by mass" and "%" means "% by mass."

[0022] First, the thickening polysaccharide is dissolved in water heated to 60 to 80°C, for example, about 70°C, using a stirrer (Step S1). The stirring speed is not particularly limited, but is preferably about 600 rpm (500 to 700 rpm). The thickening polysaccharide is added in an amount such that the thickening polysaccharide concentration is, for example, 5.0 to 30.0%. Any suitable stirrer can be used, but a suitable example is the Three-One Motor Type-600G manufactured by Shinto Scientific Co., Ltd. Calcium chloride can also be added at this time. This is because adding calcium chloride can impart a more reddish color. The amount of calcium chloride added is such that the calcium chloride concentration in the thickening polysaccharide aqueous solution or the carotenoid mixture liquid described below is, for example, 0.5 to 10.0%, preferably 0.5 to 2.0%. Calcium chloride may be added directly or as a solution in water.

[0023] on the other hand, Carotenoid oleoresin is heated to 70 to 90°C, for example, to about 80°C (step S2). Carotenoid oleoresin The color value (10%E) (hereinafter the same) of the carotenoid pigment solution is preferably about 200 to 6,500, but is not necessarily limited to this. It is sufficient to adjust the color value of the carotenoid pigment solution to about 20 to 2,000. Then, while stirring the thickening polysaccharide aqueous solution or the thickening polysaccharide aqueous solution to which calcium chloride has been added prepared in step S1 with a high-speed stirrer, the carotenoid oleoresin adjusted by heating in step S2 is added so that the color value of the carotenoid pigment solution to be finally prepared is about 20 to 2,000, and the mixture is stirred at high speed for about 5 to 15 minutes, for example, about 10 minutes (step S3). That is, Carotenoid oleoresin If the color value of is about 200 to 6,500, the carotenoid mixture Carotenoid oleoresinIf the concentration of the carotenoid pigment solution is adjusted to about 10.0 to 30.0% by mass, the final color value of the carotenoid pigment solution will be about 20 to 2,000. The stirring speed is not particularly limited, but is preferably about 7,000 rpm (5,000 to 7,000 rpm). Any suitable high-speed stirrer can be used, but a suitable example is the Homomixer MARK II manufactured by PRIMIX Corporation. The temperature of the carotenoid mixture is then adjusted to 37 to 50°C, for example, 45°C (Step S4). 。

[0024] Next, in step S4, lipase is added to the carotenoid mixture whose temperature has been adjusted to 37-50°C. The amount of lipase added is determined appropriately depending on the potency of the lipase used. For example, if the lipase used is Amano Enzyme's Lipase AY "Amano" 30SD (fat digestion capacity: 30,000 u / g), it is added at 0.05-5.0% of the carotenoid mixture (potency: approximately 15-1,500 u / g). Stirring is then continued for at least two hours while maintaining the liquid temperature at 37-50°C, preferably 45°C (step S5). The stirring speed is not particularly limited, but is preferably approximately 7,000 rpm (5,000-7,000 rpm), as in step S3. The lipase-treated liquid is then cooled to 4-10°C (step S6). The treated liquid cooled to a predetermined temperature becomes a carotenoid pigment liquid.

[0025] The color value of the carotenoid pigment solution prepared in this manner (steps S1 to S6) is preferably 20 to 2,000. This is because a color value in this range makes it easy to use. The color value measurement method is specified in the 9th edition of the Japanese Standards of Food Additives, and is a method of measuring absorbance by ultraviolet-visible absorbance measurement to measure the pigment concentration (color value) in a colorant. Usually, the color value is calculated by measuring the absorbance at the maximum absorption wavelength in the visible region of the colorant solution and converting it to the absorbance of a 10 w / v% solution, which is called the "color value E" 10% 1cm", but it is sometimes written as "color value (10%E)". [Example]

[0026] Next, several examples of the method for producing a carotenoid pigment solution according to the present invention will be described below, although the present invention is not limited to these specific examples. [Example 1] The carotenoid pigment solution (with lipase) is made up of 25.0% capsicum oleoresin, a color value (10%E) of 2,250, whose main component is capsanthin, a type of carotenoid pigment, 20.0% gum arabic as a thickening polysaccharide, 1.0% calcium chloride, 0.5% lipase (AY "Amano" 30SD, manufactured by Amano Enzyme Co., Ltd., with a fat digestive capacity of 30,000u / g), and the remainder water (53.5%), and the calcium chloride-free solution is made up of 54.5% water. A carotenoid pigment solution (without calcium chloride) containing 25.0% capsicum oleoresin, 10.0% gum ghatti thickening polysaccharides, 1.0% calcium chloride, 0.5% lipase (same as above), and the remainder water (63.5%) was also prepared (Table 1), and the differences in color were examined (Table 4-1). Each carotenoid pigment solution was prepared as follows: Gum arabic and calcium chloride were dissolved in water heated to approximately 70°C while stirring at approximately 600 rpm using a stirrer (Shinto Scientific Co., Ltd., Three-One Motor Type-600G). Meanwhile, the capsicum oleoresin was heated to approximately 80°C. Then, while stirring the gum arabic and calcium chloride solution at approximately 7,000 rpm using a high-speed mixer (Homomixer MARK II manufactured by Primix Corporation), capsicum oleoresin heated to approximately 80°C was added and mixed by stirring for approximately 10 minutes. The liquid temperature was then adjusted to approximately 45°C. Lipase was then added, and the mixture was stirred at approximately 7,000 rpm for 2 hours while maintaining the liquid temperature at approximately 45°C.

[0027] [Table 1]

[0028] [Example 2] A carotenoid pigment solution (with lipase) containing 11.0% marigold pigment oleoresin (10%E) of 6,150, mainly composed of xanthophyll, a type of carotenoid pigment, 11.0% MCT oil (medium-chain fatty acid oil), 18.0% gum arabic as a thickening polysaccharide, 1.0% calcium chloride, 0.5% lipase, and the remainder water (58.5%) was prepared in the same manner as in Example 1 (Table 2). The difference in color tone between the two solutions was examined (Table 4-2). The marigold pigment oleoresin was mixed with MCT oil (Scholet, manufactured by Nisshin Oillio Group, Inc.) and heated to approximately 80°C before use.

[0029] [Table 2]

[0030] [Example 3] A carotenoid pigment solution (with lipase) containing 18.0% Haematococcus algae pigment oleoresin with a color value (10%E) of 2,500, the main component of which is astaxanthin, a type of carotenoid pigment, 20.0% gum arabic as a thickening polysaccharide, 1.0% calcium chloride, 0.5% lipase, and the remainder water (60.5%) was prepared using the same procedure as in Example 1 (Table 3), and a carotenoid pigment solution (without lipase) containing no lipase but with the remainder water (61.0%) was prepared (Table 4-3).The differences in color tone were examined (Table 4-3).

[0031] [Table 3]

[0032] [result] The pigment solutions prepared in Example 1 were each dissolved in raw milk to a concentration of 0.5%, and the resulting color was photographed and evaluated using a spectrophotometer (SE6000, manufactured by Nippon Denshoku Industries Co., Ltd.) to obtain Lab numerical values. The smaller the b / a value, the less yellow and the more reddish, resulting in a more vivid color. The b / a value without lipase was 0.688, while the b / a value with lipase was 0.448, indicating that the presence of lipase significantly reduced the yellowness and enhanced the reddish color compared to the absence of lipase. Furthermore, the b / a value with lipase but without calcium chloride was 0.526, indicating that the presence of calcium chloride resulted in a carotenoid pigment solution with a more intense reddish color. Furthermore, when gum ghatti was used as a thickening polysaccharide, the b / a value was 0.554, resulting in a relatively reddish color, similar to the case of gum arabic.

[0033] [Table 4-1]

[0034] As in Example 1, the dye solution prepared in Example 2 was dissolved in raw milk to a concentration of 0.5%, and the resulting color was photographed and evaluated using a spectrophotometer (SE6000, manufactured by Nippon Denshoku Industries Co., Ltd.) to obtain Lab numerical values. As a result, the b / a value without lipase was 5.093, while the b / a value with lipase was 2.211, indicating that the product with lipase was redder than the product without lipase.

[0035] [Table 4-2]

[0036] As in Examples 1 and 2, the dye solution prepared in Example 3 was dissolved in raw milk to a concentration of 0.5%, and the resulting color was photographed and evaluated using a spectrophotometer (SE6000, manufactured by Nippon Denshoku Industries Co., Ltd.) to obtain Lab numerical values. As a result, the b / a value without lipase was 0.495, while the b / a value with lipase was 0.318, indicating that the product with lipase was redder than the product without lipase.

[0037] [Table 4-3]

[0038] As mentioned above, Carotenoids are dissolved in fats and oils By treating an oily liquid with lipase in the presence of a thickening polysaccharide, it is possible to obtain a carotenoid pigment solution with a strong reddish hue. Furthermore, the presence of a thickening polysaccharide promotes the enzymatic reaction of lipase, which has the effect of shortening the processing time from the conventional enzymatic reaction time of more than four hours to about two hours, approximately half the time. Furthermore, by further adding calcium chloride during the enzymatic treatment, it is possible to obtain a carotenoid pigment solution with a stronger reddish hue.

[0039] The present invention is not limited to the above-described embodiments or examples, and it goes without saying that various modifications are possible within the scope of the present invention without departing from or changing the technical concept of the present invention.

Claims

1. A method for producing a carotenoid pigment liquid, characterized by obtaining a carotenoid pigment liquid with a strong reddish color by acting lipase on an oily liquid in which carotenoids are dissolved in fats and oils in the presence of a thickening polysaccharide.

2. The method for producing a carotenoid pigment solution according to claim 1, adding the thickening polysaccharide to heated water to prepare an aqueous thickening polysaccharide solution; a step of heating the oily liquid; a step of adding the heated oily liquid to the heated aqueous solution of a thickening polysaccharide to prepare a carotenoid mixed solution; A step of allowing lipase to act on the carotenoid mixture; A method for producing a carotenoid pigment solution, comprising:

3. The method for producing a carotenoid pigment solution according to claim 2, The concentration of the thickening polysaccharide is 5.0 to 30.0 wt %, The color value (10% E) of the oily liquid is 200 to 6,500, and the concentration of the carotenoid in the carotenoid mixed liquid is 10.0 to 30.0% by mass. A method for producing a carotenoid pigment solution, comprising:

4. The method for producing a carotenoid pigment solution according to claim 2 or 3, In the step of preparing the thickening polysaccharide aqueous solution, calcium chloride is further added. A method for producing a carotenoid pigment solution, comprising:

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