Nozzle, transporter, nozzle assembly, and sample processor

Abstract

The present disclosure relates to a nozzle for a sample processor, a carrier of the nozzle for a sample processor, a nozzle assembly for a sample processor, and a sample processor. The nozzle includes a body and an orifice. The body is adapted to be loaded and held in the carrier. The carrier can be slidably inserted into the sample processor in a removable manner. An orifice is provided in the body and configured to inject a sample from the injector body in a predetermined mode. An end surface of the body is adapted to abut against an end surface of the injector body along a sample injection direction. The nozzle assembly and sample processor according to the present disclosure include the nozzle described above and a carrier.

Description

[Technical Field]

[0001] The present disclosure relates to nozzles for sample processors and sample processors that include nozzles, for example, flow cell sorters or analyzers. [Background technology]

[0002] The material in this section merely provides background information related to the present disclosure and may not necessarily constitute prior art.

[0003] Sample processors are often used to detect, analyze, and / or sort samples, such as microsomes or cells. The sample processor includes a fluidic system, a nozzle system, and a sample processing system. The nozzle system includes an injector body, within which, for example, sheath fluid and sample supplied by the fluidic system are collected, and a nozzle for injecting the sample into the injector body, for example, in a single-file array format. The sample is processed (e.g., detected, analyzed, and / or sorted) by the sample processing system while or after flowing through the nozzle system.

[0004] The nozzle typically has an orifice of 50 to 200 μm, depending on the size of the sample. During operation of the sample processor, the nozzle orifice is often blocked by the sample. At this point, the nozzle needs to be disassembled, cleaned, or replaced. However, in some existing sample processors, the nozzle and the injector body are molded together, so the entire nozzle system needs to be removed before the nozzle can be cleaned or replaced. In some existing sample processors, the nozzle is fitted into the injector body, for example, in a threaded manner. After the nozzle is repositioned or replaced, there is still a large positional deviation, which will affect the results of sample processing.

[0005] Therefore, after rearranging or replacing the entire nozzle system or a nozzle, its surrounding devices (e.g., optical devices, electronic devices, or mechanical devices) need to be adjusted, for example, to restabilize (e.g., degas) the fluid and realign the optical path. Thus, disassembly and reassembly of the nozzle assembly would be very complicated and time-consuming. In addition, rearranging or replacing the entire nozzle system or a nozzle is likely to introduce a risk of contamination.

[0006] It is therefore desirable in the art to provide a sample processor that includes a nozzle that is convenient to disassemble, clean, and assemble. Summary of the Invention [Means for solving the problem]

[0007] This section provides a general overview of the disclosure, but is not an exhaustive disclosure of the entire scope or every feature of the disclosure.

[0008] It is an object of the present disclosure to provide a nozzle for a sample processor that can be disassembled and assembled independently from the syringe body.

[0009] Another object of the present disclosure is to provide auxiliary devices that facilitate disassembly and assembly of the nozzle, such as a carrier for transporting the nozzle, a positioning member for positioning the nozzle, a support member for supporting the nozzle and biasing the nozzle toward the injector body, etc.

[0010] It is yet another object of the present disclosure to provide a sample processor that includes a nozzle that is convenient to disassemble, clean, and assemble.

[0011] According to one aspect of the present disclosure, a nozzle for a sample processor is provided. The nozzle includes a body and an orifice. The body is adapted to be loaded and held within a carrier. The carrier can be slidably inserted into the sample processor in a removable manner. The orifice is provided within an end surface of the body and is configured to inject a sample from the injector body in a predetermined mode. The end surface of the body is adapted to abut against the end surface of the injector body along a sample injection direction.

[0012] According to the nozzle of the present disclosure, it is not necessary to dispose the nozzle on the injector body (e.g., a transparent tube) upstream of the nozzle, so that various adjustment operations are omitted when repositioning the nozzle with the help of a carrier, thereby simplifying the process of repositioning the nozzle. The transparent tube upstream of the nozzle is a light-transmitting optical element. Because the nozzle of the present disclosure is connected end-to-end with the transparent tube, there is no need to make any modifications to the transparent tube, thereby significantly reducing costs. In addition, the nozzle according to the present disclosure abuts against the end surface of the injector body along the sample injection direction, so the central axis position of the injector body (e.g., the transparent tube) will not deviate, and therefore, there will be no adverse effects on optical path detection, etc.

[0013] In some embodiments according to the present disclosure, the body is configured to be removably loaded into the carrier.

[0014] In some embodiments according to the present disclosure, recesses or tabs engaged with the carrier are provided at opposing locations on the outer periphery of the body.

[0015] In some embodiments according to the present disclosure, the end surface of the body includes a groove for receiving a sealing member around the orifice.

[0016] According to another aspect of the present disclosure, there is provided a nozzle carrier for a sample processor, the carrier including a base having a receiving portion for receiving the nozzle, the base being configured to be inserted into the sample processor in an independently removable manner such that an end surface of the nozzle abuts against an end surface of the injector body along a sample injection direction.

[0017] With the aid of the carrier of the present disclosure, it is not necessary to dispose the nozzle in the injector body (e.g., a transparent tube) upstream of the nozzle, so that various adjustment operations when relocating the nozzle can be omitted, thereby simplifying the process of relocating the nozzle. In addition, according to the carrier of the present disclosure, the nozzle abuts against the end surface of the injector body along the sample injection direction, so that the nozzle will not deviate from the central axis position of the injector body (e.g., a transparent tube), and therefore will not adversely affect optical path detection, etc.

[0018] In some embodiments according to the present disclosure, the housing portion includes an elongated throughbore having a larger sized portion for loading the nozzle and a smaller sized portion for holding the nozzle.

[0019] In some embodiments according to the present disclosure, the transporter further includes a slide member that is slidable relative to the base, and a biasing member that biases the slide member toward the smaller sized portion.

[0020] In some embodiments according to the present disclosure, the end surface of the sliding member has a shape that matches the outer circumferential surface of the nozzle.

[0021] In some embodiments according to the present disclosure, the sliding member has an end that tapers towards an end surface to be engaged with a V-shaped slot of a positioning member of a sample processor.

[0022] In some embodiments according to the present disclosure, the carrier further includes notches provided on opposing side edges of the base, the notches being configured to receive the protrusions of the positioning member when the carrier is inserted into position.

[0023] In some embodiments according to the present disclosure, an inclined surface extending from the notch toward the insertion end is provided on the upper surface of the base, the inclined surface adapted to guide the protrusion of the positioning member to slide into the notch.

[0024] In some embodiments according to the present disclosure, the carrier further includes a cover configured to cover at least a portion of the base.

[0025] In some embodiments according to the present disclosure, the carrier further includes a protrusion provided on the lower surface of the base and adjacent the insertion end.

[0026] In some embodiments according to the present disclosure, the carrier further includes a locking member that locks the carrier when inserted into position.

[0027] According to yet another aspect of the present disclosure, there is provided a nozzle assembly for a sample processor, the nozzle assembly including the nozzle and / or transporter described above.

[0028] The nozzle assembly may include the nozzle and the conveyor described above, i.e., the nozzle assembly may include various features of the nozzle and the conveyor described above and may provide similar technical effects.

[0029] According to a further aspect of the present disclosure, a sample processor is provided that includes a frame, an injector body configured to receive a sample and a sheath fluid and to be secured to the frame, a nozzle located at an outlet of the injector body and having an orifice for injecting the sample into the injector body in a predetermined mode, and a carrier adapted to load and hold the nozzle and configured to be slidably inserted into the frame in a removable manner.

[0030] The nozzle assembly and sample processor may include the nozzles and transporters described above, i.e., may include various features of the nozzles and transporters described above, and may provide similar technical effects.

[0031] In some embodiments according to the present disclosure, the sample processor further includes a positioning member for positioning the nozzle, the positioning member being fixed to the frame and having a V-shaped slot, the bottom of the V-shaped slot having a shape that matches the outer periphery of the nozzle. The sample processor further includes a sliding member, the sliding member having an end that tapers toward an end surface to be engaged with the V-shaped slot of the positioning member.

[0032] In some embodiments according to the present disclosure, the nozzle is cylindrical and the curvature of the outer periphery of the nozzle exceeds that of the bottom of the V-shaped slot and that of the end surface of the sliding member.

[0033] In some embodiments according to the present disclosure, one of the mutually facing surfaces of the positioning member and the base includes a protrusion, and the other of the mutually facing surfaces of the positioning member and the base includes a notch for receiving the protrusion when the carrier is inserted into place.

[0034] In some embodiments according to the present disclosure, a sloped surface is provided on one side of the cutout, the sloped surface adapted to guide the protrusion to slide into the cutout.

[0035] In some embodiments according to the present disclosure, the sample processor further comprises a support member for supporting the base.

[0036] In some embodiments according to the present disclosure, the support member includes a fixed portion fixed to the frame and a movable portion movable relative to the fixed portion, the base being supported by the movable portion when inserted into position, and a biasing member provided between the fixed portion and the movable portion, the biasing member biasing the movable portion toward the nozzle.

[0037] In some embodiments according to the present disclosure, the movable part includes a flat inner surface for supporting the base, and a downwardly inclined surface located opposite the flat inner surface in the insertion direction of the base. The base includes a protrusion adjacent to the insertion end on a surface facing the support member. The downwardly inclined surface is adapted to guide the sliding of the protrusion.

[0038] In some embodiments according to the present disclosure, the protrusion height is greater than or equal to the height of the corresponding portion of the nozzle that protrudes from the carrier when the nozzle is loaded into the carrier.

[0039] In some embodiments according to the present disclosure, the sample processor further includes a locking member movable between a locked position and an unlocked position, and the transporter includes a locked member configured to prevent the locked member from moving in the locked position and to allow the locked member to move in the unlocked position.

[0040] In some embodiments according to the present disclosure, the locking member is rotatably disposed on the frame via a pivot, and the locked member is a pin. The present invention provides, for example, the following. (Item 1) 1. A nozzle for a sample processor, comprising: a body adapted to be loaded and held within a transporter, said transporter being slidably inserted into said sample processor in a removable manner; an orifice provided in an end surface of the body and configured to inject a sample from the injector body in a predetermined mode; Equipped with A nozzle wherein an end surface of the body is adapted to abut against an end surface of the injector body along a sample injection direction. (Item 2) Item 10. The nozzle of item 1, wherein the body is configured to be removably loaded into the carrier. (Item 3) Item 3. The nozzle of item 2, wherein recesses or tabs engaged with the carrier are provided at opposing positions on the outer circumferential surface of the body. (Item 4) 4. The nozzle of any one of claims 1-3, wherein the end surface of the body is provided with a groove for accommodating a sealing member around the orifice. (Item 5) 1. A nozzle transporter for a sample processor, comprising: A transporter comprising a base having a receiving portion for receiving the nozzle, the base being configured to be inserted into the sample processing device in a removable manner so that the end surface of the nozzle abuts against the end surface of the injector body along the sample injection direction. (Item 6) Item 6. The carrier of item 5, wherein the housing portion comprises an elongated through-hole, the elongated through-hole having a large portion for loading the nozzle and a small portion for holding the nozzle. (Item 7) a slide member that is slidable relative to the base; a biasing member that biases the sliding member toward the small-sized portion; Item 7. The transporter of item 6, further comprising: (Item 8) Item 8. The conveying device according to item 7, wherein the end surface of the sliding member has a shape that matches the outer peripheral surface of the nozzle. (Item 9) Item 9. The transporter of item 8, wherein the sliding member has an end that tapers toward the end surface to be engaged with a V-shaped slot of a positioning member of the sample processor. (Item 10) 10. The carrier of any one of items 5-9, further comprising notches provided on opposing side edges of the base, the notches configured to receive protrusions of the positioning member when the carrier is inserted into place. (Item 11) Item 11. The carrier of item 10, wherein an inclined surface is provided on the upper surface of the base extending from the notch toward the insertion end of the base, the inclined surface adapted to guide the protrusion of the positioning member to slide into the notch. (Item 12) 10. The carrier of any one of items 5-9, further comprising a cover configured to cover at least a portion of the base. (Item 13) 10. The carrier of any one of items 5-9, further comprising a protrusion provided on an undersurface of the base and adjacent an insertion end of the base. (Item 14) The carrier according to any one of items 5-9, further comprising a locking member that locks the carrier when inserted into a fixed position. (Item 15) A nozzle assembly for a sample processor, comprising a nozzle according to any one of items 1-4 and / or a transporter according to any one of items 5-14. (Item 16) 1. A sample processor comprising: The frame and an injector body configured to receive a sample and a sheath fluid and to be secured to the frame; a nozzle located at the outlet of the injector body and having an orifice for injecting the sample into the injector body in a predetermined mode; a carrier adapted to load and hold the nozzle, the carrier configured to be slidably inserted into the frame in a removable manner so that an end surface of the nozzle abuts against an end surface of the injector body along a sample injection direction; A sample processor comprising: (Item 17) Item 17. The sample processor of item 16, wherein the nozzle is removably mounted within the transporter. (Item 18) Item 18. The sample processing device of item 17, wherein the transporter comprises a base having an insertion end, the base comprising an elongated throughbore, the elongated throughbore comprising a large portion for loading the nozzle and a small portion for holding the nozzle. (Item 19) Item 19. The sample processor according to item 18, wherein recesses are provided at opposing positions on the outer circumferential surface of the nozzle, which recesses are engaged with opposing edges of the small-sized portion of the carrier. (Item 20) The carrier further comprises: a slide member that is slidable relative to the base; a biasing member that biases the sliding member toward the small-sized portion; Item 19. The sample processor according to item 18, comprising: (Item 21) 21. The sample processor according to item 20, wherein the end surface of the sliding member has a shape that matches the outer circumferential surface of the nozzle. (Item 22) the sample processor further comprises a positioning member for positioning the nozzle, the positioning member being fixed to the frame and having a V-shaped slot, the bottom of the V-shaped slot having a shape that matches the outer peripheral surface of the nozzle; the sliding member having an end tapered toward the end surface thereof for engagement with the V-shaped slot of the positioning member; 22. The sample processor according to item 21. (Item 23) the nozzle is cylindrical; the curvature of the outer peripheral surface of the nozzle exceeds that of the bottom of the V-shaped slot and that of the end surface of the sliding member; 23. The sample processor according to item 22. (Item 24) one of the surfaces of the positioning member and the base facing each other includes a protrusion; the other of the mutually facing surfaces of the positioning member and the base includes a notch for receiving the protrusion when the carrier is inserted into place; 23. The sample processor according to item 22. (Item 25) 25. The sample processor of item 24, wherein an inclined surface is provided on one side of the notch, the inclined surface adapted to guide the protrusion to slide into the notch. (Item 26) 21. The sample processor of claim 20, wherein the transporter further comprises a cover configured to cover the base and at least a portion of the sliding member. (Item 27) Item 19. The sample processor of item 18, further comprising a support member for supporting the base. (Item 28) the support member includes a fixed portion fixed to the frame and a movable portion movable relative to the fixed portion, the base is supported by the movable part when inserted into position, and a biasing member is provided between the fixed part and the movable part, the biasing member biasing the movable part towards the nozzle; Item 28. The sample processor according to item 27. (Item 29) the movable portion includes a central flat surface for supporting the base, and a downwardly inclined surface located on the opposite side of the central flat surface in the insertion direction of the base, the base includes a protrusion adjacent the insertion end on the surface facing the support member; the downwardly inclined surface is adapted to guide the sliding of the protrusion; Item 29. The sample processor according to item 28. (Item 30) 30. The sample processing device of item 29, wherein the protrusion height is greater than or equal to the height of the corresponding portion of the nozzle protruding from the transporter when the nozzle is loaded into the transporter. (Item 31) A sample processing device described in any one of items 16-30, wherein the orifice is provided axially in one end surface of the nozzle, and the end surface has a groove for accommodating a sealing member around the orifice. (Item 32) a locking member movable between a locked position and an unlocked position; The carrier includes a locked member, The locking member is configured to prevent the locked member from moving in the locked position and to allow the locked member to move in the unlocked position. 31. The sample processor according to any one of items 16-30. (Item 33) The locking member is rotatably disposed on the frame via a pivot shaft, The locked member is a pin. Item 33. The sample processor according to item 32. [Brief explanation of the drawings]

[0041] The features and advantages of one or more embodiments of the present disclosure will be more readily understood throughout the following description and by reference to the accompanying drawings, in which:

[0042] [Figure 1] FIG. 1 is a schematic cross-sectional view of a sample processor according to one embodiment of the present disclosure. [Figure 2A] 2A-2D are schematic illustrations of a nozzle assembly installation process according to an embodiment of the present disclosure. [Figure 2B] 2A-2D are schematic illustrations of a nozzle assembly installation process according to an embodiment of the present disclosure. [Figure 2C] 2A-2D are schematic illustrations of a nozzle assembly installation process according to an embodiment of the present disclosure. [Figure 2D] 2A-2D are schematic illustrations of a nozzle assembly installation process according to an embodiment of the present disclosure. [Figure 3] FIG. 3 is a perspective schematic view of a nozzle according to an embodiment of the present disclosure. [Figure 4] FIG. 4 is a schematic longitudinal cross-sectional view of the nozzle of FIG. [Figure 5] FIG. 5 is a perspective schematic top view of a transporter according to an embodiment of the present disclosure. [Figure 6] FIG. 6 is a schematic view of the transporter of FIG. 5 as viewed from another direction. [Figure 7] FIG. 7 is a perspective schematic bottom view of a transporter according to an embodiment of the present disclosure. [Figure 8] FIG. 8 is a schematic view of the transporter of FIG. 7 as viewed from another direction. [Figure 9] FIG. 9 is a schematic diagram of a carrier with a top cover removed therefrom, according to an embodiment of the present disclosure. [Figure 10A] 10A-10E are schematic diagrams showing the process of disposing a nozzle on a carrier. [Figure 10B] 10A-10E are schematic diagrams showing the process of disposing a nozzle on a carrier. [Figure 10C] 10A-10E are schematic diagrams showing the process of disposing a nozzle on a carrier. [Figure 10D] 10A-10E are schematic diagrams showing the process of disposing a nozzle on a carrier. [Figure 10E] 10A-10E are schematic diagrams showing the process of disposing the nozzles on the carrier. [Figure 11] FIG. 11 is a perspective schematic top view of a positioning member according to an embodiment of the present disclosure. [Figure 12] FIG. 12 is a perspective schematic bottom view of a positioning member according to an embodiment of the present disclosure. [Figure 13] FIG. 13 is a schematic plan view showing the cooperation of the nozzle assembly and the positioning member when inserted into position. [Figure 14A] 14A-14D are schematic diagrams showing the interaction between the carrier and the protrusions of the positioning member during the insertion process. [Figure 14B] 14A-14D are schematic diagrams showing the interaction between the carrier and the protrusions of the positioning member during the insertion process. [Figure 14C]14A-14D are schematic diagrams showing the interaction between the carrier and the protrusions of the positioning member during the insertion process. [Figure 14D] 14A-14D are schematic diagrams showing the interaction between the carrier and the protrusions of the positioning member during the insertion process. [Figure 15] FIG. 15 is a perspective schematic view of a support member according to an embodiment of the present disclosure. [Figure 16] FIG. 16 is a schematic vertical cross-sectional view of the support member of FIG. [Figure 17A] 17A-17D are schematic diagrams showing the interaction between the carrier and the support member during the insertion process. [Figure 17B] 17A-17D are schematic diagrams showing the interaction between the carrier and the support member during the insertion process. [Figure 17C] 17A-17D are schematic diagrams showing the interaction between the carrier and the support member during the insertion process. [Figure 17D] 17A-17D are schematic diagrams showing the interaction between the carrier and the support member during the insertion process. [Figure 18] FIG. 18 is a perspective schematic view of a frame according to an embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0043] Detailed Description The present disclosure will be described in detail below through exemplary embodiments with reference to the accompanying drawings. In the several drawings, like reference numerals indicate like parts and components. The following detailed description of the present disclosure is for illustrative purposes only and in no way limits the present disclosure and its application or uses. The embodiments described herein are not comprehensive and are merely some of multiple possible embodiments. The exemplary embodiments may be implemented in many different forms and should not be construed as limiting the scope of the present disclosure. In some exemplary embodiments, well-known processes, well-known device structures, and well-known technologies may not be described in detail.

[0044] 1 is a schematic longitudinal cross-sectional view of a sample processor 10 according to one embodiment of the present disclosure. The overall structure of the sample processor 10 will be described below with reference to FIG.

[0045] 1 , the sample processor 10 includes an injector body IB, a nozzle 100, a carrier 200, a positioning member 300, a support member 400, and a frame 500. The frame 500 serves as a fixed part of the sample processor 10 and is used to support and arrange the other parts. The injector body IB, the positioning member 300, and the support member 400 are directly or indirectly fixed to the frame 500. The carrier 200 is used to carry the nozzle 100 and may be inserted into the sample processor 10 (frame 500) or disassembled from the sample processor 10 (frame 500) using the nozzle 100.

[0046] The various parts of the sample processor 10 shown in Figure 1 are assembled in place and in an operational state. When the sample processor 10 is in operation, the injector body IB receives a sample from the sample line SL and a fluid, such as a sheath fluid, from the fluid line FL. The sample and sheath fluid are collected within the injector body IB and then injected through the nozzle 100. After the sample flows through the injector body IB and is injected through the nozzle 100, the sample is detected, analyzed, or sorted.

[0047] The injector body IB generally includes a cover IB1 with a sample port coupled to the sample line SL, a base IB2 with a fluid port coupled to the fluid line FL, and a transparent tube IB3 that allows light transmission for detection. The cover IB1 and the transparent tube IB3 are located on the upper and lower sides of the base IB2, respectively.

[0048] Nozzle 100 is located at the outlet of injector body IB (transparent tube IB3). Samples are injected through the nozzle, for example, in a single-file array fashion. According to the flow direction of fluids and samples, injector body IB is the upstream portion of nozzle 100. Therefore, the "injector body" referred to herein refers to the upstream portion of the nozzle where fluids such as sample and sheath fluid are collected. It should be understood that the structure of the injector body can be modified as needed and is not limited to the specific example shown in FIG. 1 .

[0049] The nozzle 100 abuts against the lower end surface of the injector body IB (transparent tube IB3) using the carrier 200. The nozzle 100 and the carrier 200 constitute the nozzle assembly of the present disclosure. The nozzle 100 is carried by the carrier 200 instead of being fitted into the injector body IB (transparent tube IB3) as in the prior art. When the carrier 200 is placed in a fixed position with the help of the positioning member 300, the support member 400, etc., the nozzle 100 is automatically aligned with the outlet of the injector body IB (transparent tube IB3). Therefore, according to the sample processor 10 of the present disclosure, assembly and disassembly of the nozzle 100 are completely independent of the injector body IB. After repositioning or replacing the nozzle 100, it is not necessary to adjust its peripheral devices (e.g., optical devices, electronic devices, or mechanical devices). For example, it is not necessary to restabilize the fluid (e.g., degas it) or realign the optical path. In this manner, disassembly and reassembly of the nozzle 100 may be significantly simplified. Also, once the carrier 200 is in place, it is not necessary to operate the nozzle 100, so the risk of contamination can be avoided or reduced.

[0050] 2A-2D are schematic diagrams of a nozzle assembly installation process according to an embodiment of the present disclosure. The nozzle assembly assembly process according to an embodiment of the present disclosure will be described below with reference to FIGS. 2A-2D.

[0051] As shown in FIG. 2A, the nozzle 100 is loaded onto the carrier 200 to form a nozzle assembly, which is located outside the sample processor 10 and ready to be inserted. As shown in FIG. 2B, the nozzle assembly is placed on the support member 400, and the nozzle assembly is inserted toward the inside of the sample processor 10 under the guidance of the support member 400. As shown in FIG. 2C, the insertion end of the nozzle assembly is inserted between the support member 400 and the positioning member 300. As shown in FIG. 2D, the outer peripheral surface of the nozzle 100 abuts against the positioning member 300, thereby inserting the nozzle assembly into place. At this moment, the nozzle 100 is supported by the support member 400 and abuts against the lower end surface of the injector body IB via the seal member 150. That is, the nozzle 100 is limited between the carrier 200, the positioning member 300, the support member 400, and the injector body IB.

[0052] The various parts of the sample processor 10 will be described in detail below.

[0053] Figure 3 is a perspective schematic view of a nozzle 100 according to an embodiment of the present disclosure. Figure 4 is a schematic longitudinal cross-sectional view of the nozzle 100 of Figure 3. The nozzle 100 will be described below with reference to Figures 3 and 4.

[0054] 3 and 4, nozzle 100 has a generally cylindrical or button-shaped body 130. Body 130 has a tubular outer periphery 131 and end surfaces 132 and 134 located at opposite ends of outer periphery 131. It should be understood that the shape of the body is not limited to the specific example shown, but may be any other suitable shape adapted to be loaded onto a carrier.

[0055] The orifice 110 is provided approximately at the center of the end surface 132. The orifice 110 extends along the axial direction. The orifice 110 is configured to inject a sample, for example, in a single-file array. The sample may be, for example, microsomes or cells. Accordingly, the diameter of the orifice 110 is approximately 50 μm to 200 μm, typically 70 μm to 100 μm. With respect to the diameter of the orifice 110, the diameter of the outer circumferential surface 131 of the body 130 may be, for example, 5.5 mm. The axial height of the orifice 110 is generally 75 μm to 125 μm. It can be seen that the size of the orifice 110 is very small. Therefore, the precision requirements for the sample processor 10 are very high, and the requirements for nozzle assembly are also very high. It should be understood that the position and size of the orifice are not limited to the specific example shown and can be changed as needed.

[0056] A hollow portion 135 that facilitates the passage of sample may be provided between the orifice 110 and an end surface (the lower end surface in the figure) 134. The hollow portion 135 has a tapered shape in the illustrated embodiment, or may have another suitable shape, for example, a cylindrical shape.

[0057] A groove 136 for accommodating a sealing member may be provided on the radially outer side of the orifice 110 on the end surface (upper end surface in the figure) 132 of the body 130. The groove 136 has an annular shape surrounding the orifice 110 for receiving a sealing member such as an O-ring. When the end surface 132 abuts against the injector body IB (transparent tube IB3), a seal between the nozzle and the injector body IB (transparent tube IB3) is achieved by the sealing member accommodated in the groove 136. It should be understood that the sealing member and the groove for accommodating the sealing member do not have to be provided on the nozzle but can also be provided on the end surface of the injector body IB (transparent tube IB3).

[0058] Recesses 133 are provided at opposing locations on the outer circumferential surface 131 of the body 130. The recesses 133 may engage with tabs or edges on the carrier 200, thereby retaining the nozzle 100 on the carrier 200. It should be understood that the feature of the nozzle 100 that engages with the carrier 200 is not limited to the recesses 133 shown in the figures, but could be, for example, a tab that engages with a recess on the carrier 200.

[0059] It should be understood that the structure of the nozzle 100 is not limited to the specific embodiment shown in the figures and can be modified as needed. The nozzle 100 may be integrally formed with the carrier 200, for example, in a molded manner. In this case, the recess 133 may be omitted or modified. Alternatively, the nozzle 100 may be loaded onto the carrier 200 in a removable manner, as will be described in detail below.

[0060] Figure 5 is a perspective schematic top view of transporter 200 according to an embodiment of the present disclosure. Figure 6 is a schematic view of transporter 200 of Figure 5 as viewed from another direction. Figure 7 is a perspective schematic bottom view of transporter 200 according to an embodiment of the present disclosure. Figure 8 is a schematic view of transporter 200 of Figure 7 as viewed from another direction. Figure 9 is a schematic view of transporter 200 with upper cover 291 removed therefrom according to an embodiment of the present disclosure. Transporter 200 will be described in detail below with reference to Figures 5-9.

[0061] 5-9, the transporter 200 includes a base 210. The base 210 is generally plate-shaped. For example, the base 210 is in the form of a rectangular plate. The base 210 has an insertion end (free end) 212 and generally parallel side edges 219 extending from the insertion end 212. When the transporter 200 is inserted, the insertion end 212 enters the sample processor 10 first. The side edges 219 may be shaped to guide the insertion of the transporter 200, for example, to guide the insertion of the transporter 200 along the frame 500.

[0062] The base 210 has a receiving portion for receiving the nozzle 100. In the embodiment shown in the figures, the receiving portion includes an elongated through-hole 211. The elongated through-hole 211 includes a large-sized portion 211a and a small-sized portion 211b. The large-sized portion 211a has a size slightly larger than the outer circumferential surface 131 of the nozzle 100 so as to load the nozzle 100. The small-sized portion 211b has a size smaller than at least a portion of the outer circumferential surface 131 of the nozzle 100 so as to hold the nozzle 100 on the carrier 200.

[0063] The larger portion 211a has a shape, e.g., an arc shape, that matches the outer circumferential surface 131 of the nozzle 100. The smaller portion 211b has opposing, generally parallel edges 213. The recesses 133 of the nozzle 100 each receive the edges 213, thereby holding the nozzle 100 on the carrier 200.

[0064] The transporter 200 further includes a sliding member 250. The sliding member 250 has a similar shape to the base 210, but a smaller size than the base 210, thereby not interfering with the insertion of the transporter 200. The sliding member 250 is capable of sliding relative to the base 210. An elongated guide slot 215 is provided in the base 210. The sliding member 250 is coupled to the base 250 via a pin 255 that is inserted into the guide slot 215. The pin 255 is capable of moving within the guide slot 215 such that the sliding member 250 slides relative to the base 210.

[0065] The end surface 251 of the sliding member 250 has a shape, for example, an arc shape, that matches the outer peripheral surface 131 of the nozzle 100. The end surface 251 of the sliding member 250 abuts against the outer peripheral surface 131 of the nozzle 100. The end surface 251 of the sliding member 250 may have a curvature slightly smaller than that of the outer peripheral surface 131, i.e., may make line contact with the outer peripheral surface 131.

[0066] The sliding member 250 may have a tapered end 253. The end 253 tapers from a side edge of the sliding member 250 toward an end surface 251. Side edges 252 and 254 of the end 253 are not parallel but are generally V-shaped. The V-shaped end 253 may engage with a V-shaped slot in a positioning member 300 of the sample processor 10 when the transporter 200 is inserted, as will be described in more detail below.

[0067] Carrier 200 may further include an upper cover 291 and a lower cover 292. Upper cover 291 and lower cover 292 constitute the covers of the present disclosure. Upper cover 291 and lower cover 292 may be made in any known manner, for example, by molding. The covers are useful for an operator to grip during transport, to protect other parts of the carrier, and also provide the operator with certain information, such as insertion direction.

[0068] The upper cover 291 and the lower cover 292 may be connected together in any known manner, for example, by screws or hinges. The base 210 and the sliding member 250 may be partially housed within the covers while the insert portions are exposed outside the covers. In an embodiment not shown, the base 210 and the sliding member 250 may be fully retracted within the covers to prevent damage during shipping.

[0069] The carrier 200 may further include a biasing member 270 (as shown in FIG. 9 ). The biasing member 270 may be housed in the cover. The biasing member 270 may be, for example, a tension spring, configured to bias the sliding member 250 toward the small-sized portion 211b. One end 271 of the biasing member 270 may be connected to the sliding member 250, and the other end 272 may be connected to the base 210 or the cover. When the sliding member 250 slides relative to the base 250, the energy stored in the biasing member 270 also changes accordingly. When the nozzle 100 is held in the small-sized portion 211b, the sliding member 250 pushes the nozzle 100 toward the insertion end 212 under the action of the biasing member 270. The type or connection mode of the biasing member 270 may be changed according to specific needs, as long as the functions described herein can be achieved.

[0070] The base 210 may also include a protrusion 214 on its lower surface adjacent the insertion end 212. The protrusion 214 is configured to slide on the support member 400, as will be described in detail below. To facilitate sliding, the protrusion 214 may have a curved shape along the insertion direction. In the illustrated embodiment, the protrusion 214 has an elongated shape extending parallel to the insertion end 212 and has an arc-shaped cross section along the insertion direction. It should be understood that the protrusion 214 may have a small spherical shape, and that multiple protrusions may be present. The configuration and number of protrusions may be varied as needed and are not necessarily limited to the specific embodiment shown in the figures.

[0071] The protrusion height of protrusion 214 may be greater than or equal to the height of the corresponding portion (lower portion) of nozzle 100 that protrudes from carrier 200 when the nozzle is loaded into smaller-sized portion 211b of carrier 200. In this way, lower end surface 134 of nozzle 100 may be protected from abrasion or interference when carrier 200 is inserted.

[0072] The protrusions 214 may be integrally formed with the base 210, or as shown, may be separately formed members connected or secured to the base 210. The formation and connection of the protrusions 214 may be varied as needed and is not necessarily limited to the specific example shown in the figures.

[0073] Notches 216 may be provided on opposing side edges 219 of base 210. The notches 216 are used to receive protrusions of positioning member 300 when carrier 200 is inserted into position, as will be described in detail below. Extending from notches 216 toward insertion end 212, angled surfaces 218 are provided on the upper surface of base 210. The angled surfaces 218 are adapted to guide the protrusions of the positioning member to slide into notches 216.

[0074] The transporter 200 may further include a locking member 280 that locks the transporter 200 when inserted into a fixed position. In the illustrated embodiment, the locking member 280 is in the form of a pin. The locking member 280 is located between the upper cover 291 and the lower cover 292, adjacent to the insertion portion of the transporter 200, and adjacent to the side edges of the upper cover 291 and the lower cover 292. The locking member 280 and a locking member 580 disposed on the frame 500 are engaged when the transporter 200 is inserted into a fixed position and locks the transporter 200. When the locking member 580 is disengaged from the locking member 280, the transporter 200 is in an unlocked state and can be removed from the sample processor 10. It should be understood that the structure, position, etc. of the locking member 280 can be changed as needed and are not necessarily limited to the specific embodiment shown in the figures.

[0075] 10A-10E are schematic diagrams illustrating the process of disposing the nozzle 100 on the carrier 200. FIG.

[0076] As shown in FIG. 10A, against the action of the biasing member 270, the sliding member 250 is pushed toward the cover until the large-sized portion 211a is exposed and receives the nozzle 100. As shown in FIG. 10B, the nozzle 100 is installed within the large-sized portion 211a. As shown in FIG. 10C, the nozzle 100 is pressed into the small-sized portion 211b so that the recess 133 of the nozzle 100 engages with the edge 213 of the small-sized portion 211b. As shown in FIG. 10D, the sliding member 250 is released, and the sliding member 250 slides and abuts against the nozzle 100 under the action of the biasing member 270. As shown in FIG. 10E, the sealing member 150 is installed within the groove 136 of the nozzle 100.

[0077] It should be understood that the step of installing the seal member 150 may occur prior to loading the nozzle 100 onto the carrier 200. It should be understood that the various steps described herein may be consistently varied and are not limited to the specific examples described herein.

[0078] Figure 11 is a perspective schematic top view of a positioning member 300 according to an embodiment of the present disclosure. Figure 12 is a perspective schematic bottom view of the positioning member 300 according to an embodiment of the present disclosure. Figure 13 is a schematic plan view showing cooperation of the nozzle assembly and the positioning member 300 when inserted into position. The positioning member 300 will be described in detail below with reference to Figures 11-13.

[0079] The positioning member 300 is fixedly disposed on the frame 500 to position the nozzle 100. As shown in Figures 11 and 12, the positioning member 300 includes a generally plate-shaped body 310. The body 310 has a slot 320 for receiving the nozzle 100 when the nozzle assembly is inserted. The slot 320 is generally V-shaped and has non-parallel side surfaces 322 and 324 and a bottom surface 321 between the side surfaces 322 and 324. The side surfaces 322 and 324 extend toward the bottom surface 321 in a tapered manner to guide the insertion of the nozzle 100 and the sliding member 250.

[0080] The bottom portion 321 has a shape, for example, an arc shape, that matches the outer periphery 131 of the nozzle 100. The bottom portion 321 may have a curvature slightly smaller than that of the outer periphery 131, i.e., may make line contact with the outer periphery 131. As explained above, the end surface 251 of the sliding member 250 may have a curvature slightly smaller than that of the outer periphery 131, i.e., may make line contact with the outer periphery 131.

[0081] 13, the nozzle 100 is sandwiched between the bottom 321 of the positioning member 300 and the end surface 251 of the sliding member 250. The curvature of the outer peripheral surface 131 of the nozzle 100 is slightly greater than that of the bottom 321 of the positioning member 300 and that of the end surface 251 of the sliding member 250, so that the nozzle 100 is easily centered.

[0082] 12, protrusions 316 may be provided on the lower surface of the body 310. The protrusions 316 may be provided symmetrically on both sides of the V-shaped slot 320. The protrusions 316 may have a curved shape, for example, an arc shape or a spherical shape, so that the protrusions 316 slide on the base 210 of the carrier 200. The protrusions 316 may be integrally formed with the body 310, or, as shown in the figure, may be a separately formed member that is connected or fixed to the body 310. The formation and connection of the protrusions 316 may be varied as needed and is not necessarily limited to the specific example shown in the figure.

[0083] The protrusion 316 is configured to slide on the upper surface of the base 210 of the carrier 200 when the nozzle assembly is inserted and prevents the upper surface 132 of the nozzle 100 and the seal member 150 from rubbing or interfering. The protrusion 316 can advance into the notch 216 when the nozzle assembly is inserted into place.

[0084] 14A-14D are schematic diagrams illustrating the interaction between the carrier 200 and the protrusion 316 of the positioning member 300 during the insertion process. As shown in FIG. 14A, the insertion end 212 of the base 210 of the carrier 200 advances between the positioning member 300 and the support member 400 and contacts the protrusion 316. As the carrier 200 is further inserted, the protrusion 316 slides onto the upper surface of the base 210 of the carrier 200, as shown in FIG. 14B. Preferably, the insertion end 212 of the base 210 may be rounded. In FIG. 14C, as the carrier 200 is about to be inserted into position, the protrusion 316 slides onto the sloped surface 218 along the base 210. When the carrier 200 is fully inserted into position, the protrusion 316 enters the notch 216, as shown in FIG. 14D.

[0085] It should be understood that the structure of the positioning member 300 is not necessarily limited to the specific embodiment shown in the figures, and may be modified as needed as long as the function of positioning the nozzle as described herein can be achieved. For example, a protrusion 316 may be provided on the upper surface of the base 210 of the carrier 200, and a notch 216 may be provided on the positioning member 300.

[0086] Figure 15 is a perspective schematic view of a support member 400 according to an embodiment of the present disclosure. Figure 16 is a schematic longitudinal cross-sectional view of the support member 400 of Figure 15. The support member 400 can not only provide a support surface for the carrier 200 to slide on, but also provide an upward biasing force for the carrier 200 such that the nozzle 100 firmly abuts against the injector body IB. The support member 400 will be described in detail below with reference to Figures 15 and 16.

[0087] 15 and 16, support member 400 includes a fixed portion 410 that is fixed to frame 500 and a movable portion 430 that is movable relative to fixed portion 410. A biasing member 470, e.g., a spring, is provided between movable portion 430 and fixed portion 410. Biasing member 470 biases movable portion 430 upward.

[0088] The fixed portion 410 has a guide surface 411 that initially contacts the transporter 200 when the nozzle assembly is inserted. The guide surface 411 may extend from the inside to the outside of the sample processor 10. Alternatively, the guide surface 411 may be completely outside the sample processor 10. The guide surface 411 may be flat or slightly curved, as long as it facilitates insertion of the transporter 200.

[0089] The movable part 430 is located inside the guide surface 411, i.e., inside the sample processing device 10. The movable part 430 may include a middle flat surface 433 for supporting the base 210, and downwardly inclined surfaces 432, 434 located on opposite sides of the middle flat surface 433 in the insertion direction of the base 210. The downwardly inclined surfaces 432, 434 are adapted to guide the sliding of the protrusion 214 of the base 210.

[0090] A through-hole 431 is provided in the inner flat surface 433 for the passage of sample injected from the nozzle 100. The through-hole 431 is positioned to be aligned with the nozzle 100 when the nozzle 100 is placed in place.

[0091] In the illustrated embodiment, two biasing members 470 are arranged on either side of the through-hole 431. It should be understood that the structure, arrangement, and number of biasing members 470 may be varied as needed and are not necessarily limited to the specific embodiment shown in the figures.

[0092] In the free state, i.e., without the nozzle assembly inserted, the biasing member 470 may cause the inner flat surface 433 of the movable part 430 to be slightly higher than the guide surface 411 of the fixed part 410.

[0093] When the nozzle assembly is inserted, the carrier 200 presses the movable part 430 downward against the action of the biasing member 470. At this moment, energy is stored in the biasing member 470. When the nozzle assembly is inserted into place, the biasing member 470 uses the stored energy to apply an upward biasing force to the carrier 200, and hence the nozzle 100 via the movable part 430, thereby firmly abutting the nozzle 100 against the injector body IB.

[0094] 17A-17D are schematic diagrams showing the interaction between the transporter 200 and the support member 400 during the insertion process. In FIG. 17A, the transporter 200 is placed on the guide surface 411 of the support member 400. Specifically, the protrusion 214 of the transporter 200 is placed on the guide surface 411 and inserted into the sample processor 10 under the guidance of the guide surface 411. Due to the presence of the protrusion 214, the lower end surface 134 of the nozzle 100 does not come into contact with the support member 400, thereby preventing the lower end surface 134 from being worn or interfered with. In FIG. 17B, the protrusion 214 comes into contact with the downwardly inclined surface 434 and pushes the movable part 430 down while sliding upward under the guidance of the downwardly inclined surface 434. In FIG. 17C, the protrusion 214 further slides onto the inner flat surface 433, and the movable part 430 is now in a depressed state. 17D, protrusion 214 slides over downwardly inclined surface 432. At this moment, under the action of biasing member 470, movable part 430 moves upward and abuts against the lower end surface of nozzle 100, thereby bringing nozzle 100, via seal member 150, into tight abutment against injector body IB.

[0095] 18 is a perspective schematic diagram of a frame 500 according to an embodiment of the present disclosure. The frame 500 provides support and arrangement for various portions of the sample processor 10. Thus, the structure of the frame 500 can be modified according to different configurations and different arrangements of the various portions of the sample processor 10. The portions of the frame 500 related to the nozzle assembly of the present disclosure will be described in detail below with reference to FIG. 18.

[0096] 18, frame 500 includes parallel side walls for receiving and arranging positioning member 300 and support member 400. Locking member 580 is rotatably disposed on frame 500 via pivot 520. Locking member 580 is configured to be movable between a locked position (as shown in FIG. 13), in which movement of locked member 280 is prevented, and an unlocked position (as shown in FIGS. 2A and 2B), in which locked member 280 is released.

[0097] The locking member 580 includes a locking end 582 that cooperates with the locked member 280 in the locked position, and a free end 584 opposite the locking end 582. The locking member 580 further includes a stop portion 586. When the locking member 580 is in the released position, the stop portion 586 is stopped by the end 560 of the side wall of the frame 500.

[0098] Referring to Figure 13, when the nozzle assembly is inserted into position, the locked member 280 moves over the locking end 582 of the locking member 580 and is therefore stopped by the locking end 582 and cannot move outward.

[0099] When it is necessary to disassemble the nozzle assembly, the free end 584 of the locking member 580 is first operated to pivot to the release position. At this moment, the nozzle assembly (carrier 200) can be pulled outward. The process of pulling the nozzle assembly (carrier 200) outward is opposite to the process of inserting the nozzle assembly (carrier 200) described above, and will not be described in detail here.

[0100] Although the present disclosure has been described with reference to exemplary embodiments, it should be understood that the present disclosure is not limited to the specific embodiments described and illustrated herein. Those skilled in the art can make various modifications to the exemplary embodiments without departing from the scope defined by the claims. Unless there is a contradiction, features in various embodiments can be combined with each other. Or, certain features in an embodiment may also be omitted.

Claims

1. 1. A nozzle transporter for a sample processor, comprising: a base having a receiving portion for receiving the nozzle, the base being configured to be removably inserted into the sample processor such that an end surface of the nozzle abuts against an end surface of the syringe body along a sample injection direction; the housing portion comprises an elongated through-hole, the elongated through-hole having a large sized portion for loading the nozzle and a small sized portion for holding the nozzle; The carrier further comprises a slide member slidable relative to the base and a biasing member biasing the slide member toward the smaller sized portion.

2. 2. The transporter of claim 1, wherein the end surface of the sliding member has a shape that matches the outer circumferential surface of the nozzle.

3. 3. The carrier of claim 1 or claim 2, further comprising a cover configured to cover at least a portion of the base.

4. 3. The carrier of claim 1 or claim 2, further comprising a protrusion provided on a lower surface of the base and adjacent an insertion end of the base.

5. 3. The carrier according to claim 1, further comprising a locking member that locks the carrier when inserted into a fixed position.

6. 1. A nozzle assembly for a sample processor, comprising: A conveyor according to any one of claims 1 to 5; A nozzle, a body adapted to be loaded and held within the transporter, the transporter being slidably inserted into the sample processor in a removable manner; an orifice provided in an end surface of the body and configured to inject a sample from the injector body in a predetermined mode; a nozzle; Equipped with A nozzle assembly, wherein an end surface of the body is adapted to abut against an end surface of the injector body along a sample injection direction.

7. The nozzle assembly of claim 6 , wherein the body is configured to be removably loaded into the carrier.

8. The nozzle assembly of claim 7 , wherein recesses or tabs engaged with the carrier are provided at opposing locations on the outer circumferential surface of the body.

9. A nozzle assembly according to any one of claims 6 to 8, wherein the end surface of the body is provided with a groove for receiving a sealing member around the orifice.

10. 1. A sample processor comprising: The frame and an injector body configured to receive a sample and a sheath fluid and to be secured to the frame; A nozzle assembly according to any one of claims 6 to 9. Equipped with the nozzle is located at the outlet of the syringe body; A sample processing device, wherein the transporter is adapted to load and hold the nozzle, and the transporter is configured to be slidably inserted into the frame in a removable manner so that the end surface of the nozzle abuts against the end surface of the injector body along the sample injection direction.

11. A sample processor as described in claim 10, wherein a recess on the outer peripheral surface of the nozzle engages with an opposing edge of a small-sized portion of the conveyor.

12. the sample processor further comprises a positioning member for positioning the nozzle, the positioning member being fixed to the frame and having a V-shaped slot, the bottom of the V-shaped slot having a shape that matches the outer circumferential surface of the nozzle; 11. The sample processor of claim 10, wherein the sliding member has an end that tapers toward its end surface for engagement with the V-shaped slot of the positioning member.

13. the nozzle is cylindrical; 13. The sample processor of claim 12, wherein the curvature of the outer peripheral surface of the nozzle exceeds that of the bottom of the V-shaped slot and that of the end surface of the sliding member.

14. one of the mutually facing surfaces of the positioning member and the base includes a protrusion; 13. The sample processor of claim 12, wherein the other of the mutually facing surfaces of the positioning member and the base includes a notch for receiving the protrusion when the transporter is inserted into place.

15. 15. The sample processor of claim 14, wherein an inclined surface is provided on one side of the notch, the inclined surface adapted to guide the protrusion to slide into the notch.

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