Skin care products and food and beverages

Abstract

To provide an active ingredient for an external preparation for skin or food and drink that is made from natural products and has excellent biological safety and stability, and also has excellent skin penetrability and metabolism.SOLUTION: The present invention provides an external preparation for skin and food and drink comprising, as an active ingredient, the collagen fermented with a microorganism having one or more enzymatic activities selected from protease activity and peptidase activity.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation and a food or drink containing a fermented product of collagen as an active ingredient. [Background technology]

[0002] In recent years, various studies have been conducted on skin aging, such as wrinkles and sagging, and active ingredients that can adequately protect the skin from these external factors are needed. Conventionally, collagen or its derivatives, which have moisturizing effects, have been widely used as active ingredients in topical skin preparations and foods and beverages, but there has been a problem in that they do not exert sufficient effects when applied to the skin.

[0003] [Patent Document 1] Patent Publication No. 2012-087091 [Patent Document 2] Patent Publication No. 2015-504440 [Patent Document 3] Patent Publication No. 2019-085331 [Patent Document 4] Patent Publication No. 2019-136027 DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0004] As a result of intensive research conducted by the inventors in light of the above-mentioned conventional problems, they discovered that fermented products obtained by fermenting collagen with microorganisms having protease activity or aminopeptidase activity are useful as ingredients in topical skin preparations and foods and beverages. [Means for solving the problem]

[0005] The present invention relates to an external skin preparation containing, as an active ingredient, a product of fermentation of collagen by a microorganism having one or more enzyme activities selected from the group consisting of protease activity and peptidase activity. The present invention also provides a food or drink containing, as an active ingredient, a product of fermentation of collagen by a microorganism having at least one enzyme activity selected from the group consisting of protease activity and peptidase activity. The present invention also provides a method for producing a fermented product, which comprises a step of fermenting collagen with a microorganism having at least one enzyme activity selected from the group consisting of protease activity and peptidase activity. [Effects of the Invention]

[0006] According to the present invention, it is possible to provide an external skin preparation and a food or beverage product that has excellent skin permeability, promotes the expression of collagen in the skin, and exhibits effects such as improving skin firmness, preventing or improving wrinkles, and preventing or improving age spots and freckles. [Brief explanation of the drawings]

[0007] [Figure 1] FIG. 1 is a diagram showing the expression rate of type I collagen gene in the fermented product according to the present invention. [Figure 2] FIG. 2 is a diagram showing the expression rate of type IV collagen gene in the fermented product according to the present invention. [Figure 3] FIG. 3 is a diagram showing the expression rate of type III collagen gene in the fermented product according to the present invention. [Figure 4] FIG. 4 is a diagram showing the expression rate of type IIV collagen gene in the fermented product according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0008] Preferred embodiments of the present invention will now be described in detail. The present invention relates to an external skin preparation or a food or drink containing, as an active ingredient, a product of fermentation of collagen by a microorganism having at least one enzyme activity selected from the group consisting of protease activity and peptidase activity.

[0009] The collagen of the present invention can be any collagen that can be incorporated into external skin preparations or foods and beverages, including, but not limited to, fish-derived collagen and jellyfish-derived collagen. Fish-derived collagen includes salmon, tuna, bonito, shark, sturgeon, alfonsino, albatross, sea bream, red snapper, sea bass, yellowtail, cod, flounder, flatfish, pufferfish, eel, conger eel, conger eel, filefish, tilapia, squid, etc.

[0010] In the present invention, examples of microorganisms having protease activity or aminopeptidase activity that degrades collagen include Labyrinthulea, Streptomyces, Bacillus, and Cyanobacteria.

[0011] Examples of Labyrinthula microorganisms include the genera Aurantiochytrium, Schizochytrium, Sicyoidochytrium, Thraustochytrium, and Parietichytrium of the Thraustochytriaceae family, and the genus Oblongichytrium of the Oblongichytriidae family. These Labyrinthula microorganisms are available, for example, from the National Bioresource Center (NBRC). NBRC102164 (Aurantiochytrium sp.) and NBRC102615 (Schizochytrium sp.) are available from the National Institute of Technology and Evaluation (NBRC).

[0012] Examples of actinomycetes (genus Streptomyces) include Streptomyces albus, Streptomyces 99-GP-2D-5 strain, and Streptomyces griseus.

[0013] Examples of Bacillus subtilis (genus Bacillus) include Bacillus natto, Bacillus subtilis, and Bacillus circulans.

[0014] The method for fermenting collagen is not particularly limited, as long as it uses a collagen-containing medium and the conditions are such as static culture, neutral culture at a constant pH, batch culture, and continuous culture, allowing the cells to grow well.

[0015] If necessary, the medium may contain, for example, glucose, starch, or dextrin as a carbon source, peptone as a nitrogen source, and artificial seawater containing vitamins and minerals.

[0016] The fermentation temperature is generally 25°C to 35°C, but the present invention is not limited to this.

[0017] The fermentation time is generally 24 to 168 hours, but the present invention is not limited to this.

[0018] Before the fermentation treatment, the microorganisms may be pre-cultured to activate their proliferation ability.

[0019] The fermented product obtained by the above fermentation treatment preferably contains peptides with a molecular weight of 450 to 1500. In particular, it is preferable that the fermented product contains peptides with a molecular weight of 950 to 1000, and peptides with a molecular weight of 1100 to 1250.

[0020] After the fermentation treatment is completed, the fermented product can be used as it is as an ingredient of an external skin preparation or a food or drink, but may be subjected to sterilization, deodorization, decolorization or concentration treatment as necessary.

[0021] The fermented product to be incorporated into the external skin preparation or food or drink may be in any form, such as liquid or powder.

[0022] The fermented product of the present invention can be incorporated into, for example, external preparations for the skin (including the scalp) (cosmetics, quasi-drugs, external pharmaceuticals), or foods and beverages for beauty or health promotion. Examples of external preparations for the skin include emulsions, creams, lotions, essences, packs, sheet masks, lipsticks, foundations, liquid foundations, pressed makeup powders, blushers, face powders, facial cleansers, body shampoos, scalp and hair shampoos, hair conditioners, shampoos or tonics for hair growth and care, cleansing cosmetics such as soaps, and bath additives, but the present invention is not limited to these. In addition, as food and beverage products for beauty or health promotion, they can be blended into beverages such as beauty drinks, nutritional drinks, sports drinks, near water, vitamin drinks, mineral drinks, and alcoholic drinks; foods such as various soups (including powdered soups), dairy products, jellies, candies, candy tablets, and gum; and health foods and beverages in tablet, liquid, granular, or jelly form, but the present invention is not limited to these, and they can also be blended into foods and beverages that can be taken orally.

[0023] When the fermented product of the present invention is incorporated into a topical skin preparation or food or drink, in addition to the composition as an essential ingredient, ingredients commonly used in such products can be incorporated as needed, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, anti-inflammatory agents, antiseptics / bactericides, cell activators, anti-acne agents, powder ingredients, UV absorbers, antioxidants, whitening agents, pigments, fragrances, chelating agents, etc. Furthermore, as long as the efficacy and characteristics of the composition are not impaired, there is no problem in incorporating other physiologically active ingredients in combination.

[0024] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples of suitable glycerides include hydrocarbons such as cocos and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0025] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0026] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0027] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, various amino acids and their derivatives.

[0028] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0029] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0030] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. Examples include chrysoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0031] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0032] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0033] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0034] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0036] Further, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0037] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzates thereof.

[0038] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc.; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is hexanoyl, octanoyl, decanoyl, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc.; L-ascorbic acid tetrafatty acid esters such as 3-isopalmitate, ... -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), and tranexamic acid amides (e.g., tranexamic acid methylamide). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0039] Anti-wrinkle agents include vitamin A or its derivatives, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, ascorbic acid phosphate magnesium salt, etc.), pantothenyl alcohol, tranexamic acid, and nicotinamide.

[0040] Furthermore, the following animal-, plant- or microorganism-derived components can also be used in combination. For example, collagen or a hydrolysate thereof, yeast extract or hydrolysate, lactic acid bacteria culture, Gramineae plants, Cruciferae plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Umbelliferae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, Umbellifer ... Examples include extracts of one or more plants selected from the family Ruciaceae, Garciniaceae, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweed selected from the family Laminariaceae, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0041] As ingredients derived from grass plants, particularly preferred are rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice fermentation liquid, sake lees extract derived from sake, bamboo shoot skin extract from Madake or Moso bamboo, and fermented Job's tears seed. Furthermore, as Brassicaceae plants, particularly preferred are seed extracts or hydrolysates or fermented products of Hakugai, Ogai, or Kokugai. Furthermore, as ingredients derived from Theaceae plants, particularly preferred are green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.). As ingredients derived from Rosaceae plants, particularly preferred are Damask rose flower extract, peach flower, leaf, or immature fruit extract, apricot fruit or seed extract, strawberry flower extract, and cherry blossom flower or leaf extract. Furthermore, as components derived from Paeoniaceae plants, extracts of peony root or flower and peony flower or root are preferred. As components derived from Amaranthaceae plants, Salicornia extract is particularly preferred. As components derived from Umbelliferae plants, Cnidium extract, Centella asiatica extract, or Pimpinella extract are preferred. As components derived from Zosteraea plants, extracts of Zostera marina or Zostera kohlrabi are particularly preferred. As components derived from Leguminosae plants, extracts of white soybeans or black soybeans or their hydrolysates, fermented soy milk broth, adzuki bean extract, red clover extract, and pueraria lobata root extract are particularly preferred. As components derived from Asteraceae plants, burdock root extract, sunflower sprout extract, Arctium gracilis extract, arnica extract, chamomile flower extract, daisy extract, or Japanese yarrow extract are particularly preferred. As components derived from Malvaceae plants, fermented products of hibiscus, rose of sharon, or hibiscus are particularly preferred. As a component derived from a plant of the Gentianaceae family, gentian extract is preferred. As a plant of the Lamiaceae family, perilla extract and Veronica barbata fruit extract are preferred. As a component derived from a plant of the Nelumbo nucifera family, lotus flower or lotus seed extract or fermented lotus seed product are particularly preferred. As a component derived from a plant of the Cucurbitaceae family, loofah extract is particularly preferred. As a component derived from a plant of the Araliaceae family, an extract or fermented product of Panax ginseng is preferred. As a component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) can be mentioned.As a component derived from a plant of the Bignoniaceae family, pau d'arco bark extract is preferred. As a component derived from a plant of the Actinidiaceae family, immature kiwi extract is preferred. As a component derived from a plant of the Moraceae family, Morus alba extract, mulberry fruit extract, and fig fruit or bark extract are preferred. As a component derived from a plant of the Rhamnaceae family, jujube fruit extract is preferred. Furthermore, as a component derived from a plant of the Iridaceae family, saffron is preferred. As a component derived from a plant of the Campanulaceae family, an extract or hydrolysate of Codonopsis globulus root is preferred. As a component derived from a plant of the Anacardiaceae family, mango fruit extract is particularly preferred. As a component derived from a plant of the Garciniaceae family, mangosteen fruit extract is particularly preferred. Furthermore, as a component derived from a plant of the Valenciaceae family, cherimoya fruit extract is preferred. Preferred examples of components derived from Rutaceae plants include Satsuma mandarin, bergamot fruit extract, grapefruit or banpeiyu fruit (including immature fruit), extracts containing flavonoids and their glycosides contained in plants such as grapefruit or hassaku, and Japanese pepper seed extract. Preferred examples of components derived from Liliaceae plants include extracts of daylilies, daylilies, Casablanca lilies, Madonna lilies, Japanese lilies, and tulips. Preferred examples of components derived from Crassulaceae plants include extracts or fermented products of Rhodiola rosea. Preferred examples of components derived from Oleaceae plants include jasmine flower extract. Preferred examples of Cupressaceae plants include Juniperus communis fruit extract. Preferred examples of components derived from Myrtaceae plants include guava leaf extract. Preferred examples of components derived from Orchidaceae plants include extracts of Bletilla sieboldii (White Orchid). As the component derived from a plant of the Convolvulaceae family, a sweet potato extract or its fermented product, or a sweet potato shochu lees extract or its fermented product is preferred. As the component derived from seaweed of the Laminaria family, a kelp extract is particularly preferred. As the component derived from seaweed of the Mirinaceae family, a Katamen-kirinsai extract is particularly preferred. As the component derived from seaweed of the Ulva family, an Ulva pertusa extract is particularly preferred. As the component derived from seaweed of the Funoriaceae family, a Funori extract is particularly preferred.

[0042] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0043] Production Example 1. Preparation of fermented product (1) A medium containing five times the volume (1800 g) of artificial seawater was added to 360 g of conger eel skin and sterilized by heating. 18 mL of a previously prepared Labyrinthula (Aurantiochytrium) preculture solution was inoculated and cultured statically at 30°C for 5 days. After the culture was completed, the medium was sterilized by heating, and the culture solution was filtered, deodorized, decolorized, and desalted to obtain 2.5 kg of a nearly colorless, transparent fermented solution (solid concentration 1.1%).

[0044] Production Example 2. Preparation of fermented product (2) 2.7 kg (solid concentration 1.3%) was obtained in the same manner as in Production Example 1, except that salmon skin was used instead of the conger eel skin.

[0045] Preparation of fermented product (3) of Production Example 3 2.4 kg (solid content concentration 1.0%) was obtained in the same manner as in Production Example 1, except that sea bream skin was used instead of conger eel skin.

[0046] Preparation of fermented product (4) of Production Example 4 2.6 kg (solid content concentration 1.1%) was obtained in the same manner as in Production Example 1, except that South Sea bream skin was used instead of conger eel skin.

[0047] Preparation of fermented product (5) of Production Example 5 2.4 kg (solid content concentration 1.0%) was obtained in the same manner as in Production Example 1, except that tilapia skin was used instead of conger eel skin.

[0048] Preparation of fermented product (6) of Production Example 6 2.6 kg (solid content: 1.3%) was obtained in the same manner as in Production Example 1, except that Matsudai skin was used instead of conger eel skin.

[0049] Production Example 7. Preparation of fermented product (7) 2.5 kg (solid concentration 1.1%) was obtained in the same manner as in Production Example 1, except that Labyrinthula (Schizochytrium) was used instead of Labyrinthula (Aurantiochytrium).

[0050] Production Example 8. Preparation of fermented product (8) 2.3 kg (solid concentration 1.0%) was obtained in the same manner as in Production Example 1, except that Labyrinthulea (Schioidochytrium) was used instead of Labyrinthulea (Aurantiochytrium).

[0051] Production Example 9. Preparation of fermented product (9) 2.2 kg (solid concentration 1.0%) was obtained in the same manner as in Production Example 1, except that Streptomyces was used instead of Labyrinthula (Aurantiochytrium).

[0052] Production Example 10. Preparation of fermented product (10) 2.4 kg (solid concentration 1.1%) was obtained in the same manner as in Production Example 1, except that Bacillus subtilis was used instead of Labyrinthulea (Aurantiochytrium).

[0053] Production Example 11. Preparation of fermented product (11) 2.1 kg (solid concentration 0.9%) was obtained in the same manner as in Production Example 1, except that cyanobacteria were used instead of Labyrinthula (Aurantiochytrium).

[0054] Comparative Example 1. Preparation of hydrolyzed collagen A collagen dispersion was prepared by dispersing 5 times the amount (1800 g) in salmon skin (360 g), and 0.01 wt% hydrolyzed papain was added to this solution, followed by enzymatic hydrolysis with stirring for 1 hour while keeping the temperature at 40-50°C. After hydrolysis was complete, the temperature was raised to 80°C and kept at that temperature for 30 minutes to inactivate the enzyme, and the solution was filtered to obtain 2.0 kg of hydrolyzed collagen solution with a concentration of 1%.

[0055] Test Example 1: Analysis of fermented product components The fermented product of Production Example 1 was subjected to mass spectrometry as follows. 1 μL of the fermented product solution was mixed with 1 μL of a matrix (α-cyano-4-hydroxycinnamic acid) solution, and 1 μL of this mixture was spotted onto a sample plate. The matrix solution used was prepared using a method commonly used for mass spectrometry. After the sample was thoroughly dried, the sample plate was placed in the analyzer. A laser ionization time-of-flight mass spectrometer (manufactured by SIMADZU) was used for the analysis. Various analytical parameters were adjusted appropriately, and the analysis was performed under conditions of laser intensity that provided the best ionization efficiency.

[0056] As a result of mass spectrometry, peaks were confirmed at molecular weights of 950 to 1000 and 1100 to 1250. This confirmed that the fermented product according to the present invention contained peptides with the above molecular weights.

[0057] Test Example 2: Evaluation test of collagen gene expression recovery effect The effect of restoring the expression of type I collagen gene (COL1A1) and type IV collagen gene (COL4A1) in cells irradiated with ultraviolet light was evaluated by the following method. Normal human fibroblasts were cultured at 6 × 10 in Eagle's minimum essential medium containing 0.5% NCS. 4The cells were prepared at a concentration of 1 / mL, seeded at 1 mL per well on a 24-well plate, and cultured at 37°C under 5% CO2 and saturated steam. After 24 hours of culture, the cells were irradiated with 20 mJ / cm2 of UVB from the bottom, and then culture medium containing the collagen fermentation broth of Production Examples 1 to 11 (a collagen fermentation broth of any one of Production Examples 1 to 11 was added to the culture medium so that the final concentration of the solution was 2.0% relative to the total culture medium volume) was added as a sample of the present invention. As a comparative control, a culture medium containing only 30% butylene glycol solution (a final concentration of 30% butylene glycol relative to the total culture medium volume was adjusted to 2.0%) was added instead of the sample of the present invention (control group). After another 24 hours, the cells from each test group were harvested with 0.5 mL of Trizol reagent (Invitrogen). The collected cells were mixed with 100 μL of chloroform (Wako Pure Chemical Industries, Ltd.), stirred, and centrifuged at 15,000 rpm and 4°C for 15 minutes in a centrifuge (TOMY MX-160). 200 μL of the aqueous layer was then removed. 250 μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected aqueous layer, stirred, and centrifuged at 15,000 rpm and 4°C for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, which was washed by stirring. The precipitate was then collected by centrifugation at 15,000 rpm and 4°C for 15 minutes. The collected total RNA was reverse-transcribed to synthesize cDNA using a designated kit [PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio Inc.)]. Using the synthesized cDNA as a sample, the expression of various genes and the expression of the internal standard beta-actin gene were detected using Thermal Cycler Dice® Real Time System Single (manufactured by Takara Bio Inc.) and SYBR® Premix Ex Taq™ II (Perfect Real Time) (manufactured by Takara Bio Inc.).Here, β-actin is one of the housekeeping genes (a gene that is expressed at a constant level in many tissues and cells, is constantly expressed, and is essential for cell maintenance and proliferation), and because its expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of each gene in each test group when the expression level of the β-actin gene was held constant. In this test system, the expression level of each gene in the control group, which was not exposed to UV light, was set at 100, and the relative expression level of that gene in the other test groups was calculated.

[0058] The results of Test Example 2 are shown in Figures 1 and 2. As shown in Figures 1 and 2, it was confirmed that the fermented product of the present invention has the effect of restoring the expression of type I collagen gene (COL1A1) and type IV collagen gene (COL4A1) that had been reduced by UV irradiation. Type I collagen is the most abundant collagen in the dermis and functions to maintain skin firmness, while type IV collagen is a component of the basement membrane and functions as the foundation of the epidermis. This suggests that they have the effect of maintaining skin structure and firmness. Furthermore, it is also suggested that they may prevent and improve wrinkles. Furthermore, since the basement membrane serves as the foundation of the epidermis, contributing to the normal functioning of epidermal cells, which in turn promotes normal epidermal turnover, the fermented product of the present invention restores the expression of type IV collagen that had been reduced by UV irradiation, thereby normalizing epidermal turnover and thereby prompting the rapid excretion of melanin synthesized in the cells, i.e., exhibiting a whitening effect.

[0059] Test Example 3: Evaluation test of collagen gene expression promoting effect The effects of promoting the expression of type III collagen gene (COL3A1) and type IIV collagen gene (COL7A1) were evaluated by the following method. Normal human fibroblasts were cultured at 6 × 10 in Eagle's minimum essential medium containing 0.5% NCS. 4The cells were prepared at a concentration of 1 / mL, seeded in 24-well plates at 1 mL per well, and cultured at 37°C under 5% CO2 and saturated steam. After 24 hours of culture, a culture medium containing a fermentation product from any one of Production Examples 1 to 11 or Comparative Production Example 1 was added as a sample of the present invention (a fermentation product from any one of Production Examples 1 to 11 was added so that the final concentration of the solution was 2.0% relative to the total volume of the culture medium), and the cells were cultured. As a comparative control, a test group (control group) was set up in which a culture medium containing only a 30% butylene glycol solution (the final concentration of 30% butylene glycol relative to the total volume of the culture medium was adjusted to 2.0%) was added instead of the sample of the present invention. After a further 48 hours, the cells from each test group were harvested with 0.5 mL of Trizol reagent (Invitrogen). The collected cells were mixed with 100 μL of chloroform (Wako Pure Chemical Industries, Ltd.) and vortexed. The mixture was then centrifuged at 15,000 rpm and 4°C for 15 minutes in a centrifuge (TOMY MX-160). 200 μL of the aqueous layer was then removed. 250 μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected aqueous layer and vortexed. The mixture was then centrifuged at 15,000 rpm and 4°C for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, which was then washed with vortexing. The precipitate was then collected by centrifugation at 15,000 rpm and 4°C for 15 minutes. The collected total RNA was reverse transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio Inc.) to synthesize cDNA. Using the synthesized cDNA as a sample, the expression of various genes and the expression of the internal standard beta-actin gene were detected using Thermal Cycler Dice® Real Time System Single (manufactured by Takara Bio Inc.) and SYBR® Premix Ex Taq™ II (Perfect Real Time) (manufactured by Takara Bio Inc.).Here, β-actin is one of the housekeeping genes (a gene that is expressed at a constant level in many tissues and cells, is constantly expressed, and is essential for cell maintenance and proliferation), and because its expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of each gene in each test group when the expression level of the β-actin gene was held constant. In this test system, the expression level of each gene in the control group was set to 100, and the relative expression level of that gene in other test groups was calculated.

[0060] The results of Test Example 3 are shown in Figures 3 and 4. As shown in Figures 3 and 4, it was confirmed that the fermented product of the present invention has an excellent effect of promoting the expression of type III collagen gene (COL3A1) and type IIV collagen gene (COL7A1) compared to the hydrolyzed product of the Comparative Production Example. Type III collagen is present in the dermis and, together with type I collagen, maintains skin firmness. Type IIV collagen is a component of the basement membrane and functions to maintain its bond with the dermis, suggesting that it maintains skin structure and firmness. Furthermore, it is also suggested that it may prevent and improve wrinkles. Furthermore, since the basement membrane serves as the foundation of the epidermis and contributes to the normal functioning of epidermal cells, which in turn promotes normal epidermal turnover, it is suggested that the fermented product of the present invention promotes the expression of type IV collagen, thereby normalizing epidermal turnover and thereby rapidly eliminating melanin synthesized in cells, thereby exhibiting a whitening effect.

[0061] Since the fermented product of the present invention has the above-mentioned effects, it is suggested that a synergistic effect in improving wrinkles can be achieved by using it in combination with anti-wrinkle agents such as vitamin A or its derivatives, vitamin E or its derivatives (such as dl-α-tocopherol acetate), pantothenyl alcohol, tranexamic acid, and nicotinamide.

[0062] Prescription example 1. Lotion [Ingredients] Part Eucalyptus oil 0.2 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Fermented product of Production Example 1 2.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Stearyl glycyrrhizinate 0.05 Isopropylmethylphenol 0.1 Align In 0.1 D-Pantothenyl alcohol 0.1 Salicylic acid 0.5 Urea 5.0 l-menthol 0.9 dl-menthol 0.2 1,3-butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Hinokitiol 0·003 Photosensor No. 201 0.002 Purified water (enough to make the total volume 100 parts)

[0063] Prescription example 2: Lotion A lotion was obtained with the same composition as Formulation Example 1, except that 2.0 parts of the fermented product of Production Example 2 was added instead of the fermented product of Production Example 1.

[0064] Prescription example 3: Lotion A lotion was obtained with the same composition as Formulation Example 1, except that 2.0 parts of the fermented product of Production Example 3 was added instead of the fermented product of Production Example 1.

[0065] Prescription example 4: Lotion A lotion was obtained with the same composition as Formulation Example 1, except that 2.0 parts of the fermented product of Production Example 4 was added instead of the fermented product of Production Example 1.

[0066] Prescription example 5. Lotion A lotion was obtained with the same composition as Formulation Example 1, except that 2.0 parts of the fermented product of Production Example 5 was added instead of the fermented product of Production Example 1.

[0067] Prescription example 6. Lotion [Ingredients] Part Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Cetyl alcohol 2.0 Behenyl Alcohol 2.0 Methylparaben 0.1 Fermented product of Production Example 6 2.0 Ascorbic Acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc Oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-butylene glycol 5.0 Potassium hydroxide 0.5 Purified water (enough to make the total volume 100 parts)

[0068] Prescription example 7. Lotion A lotion was obtained with the same composition as in Formulation Example 1, except that, among the ingredients of the lotion in Formulation Example 6, 2.0 parts of the fermented product of Production Example 7 was blended in place of the fermented product of Production Example 6.

[0069] Prescription example 8. Lotion A lotion was obtained with the same composition as in Formulation Example 1, except that, among the ingredients of the lotion in Formulation Example 6, 2.0 parts of the fermented product of Production Example 8 was blended in place of the fermented product of Production Example 6.

[0070] Prescription example 9. Lotion A lotion was obtained with the same composition as in Formulation Example 1, except that, among the ingredients of the lotion in Formulation Example 6, 2.0 parts of the fermented product of Production Example 9 was blended in place of the fermented product of Production Example 6.

[0071] Prescription example 10. Lotion A lotion was obtained with the same composition as in Formulation Example 1, except that, among the ingredients of the lotion in Formulation Example 6, 2.0 parts of the fermented product of Production Example 10 was blended in place of the fermented product of Production Example 6.

[0072] Prescription example 11. Lotion A lotion was obtained with the same composition as in Formulation Example 1, except that, among the ingredients of the lotion in Formulation Example 6, 2.0 parts of the fermented product of Production Example 11 was blended in place of the fermented product of Production Example 6.

[0073] Prescription example 12. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Fermented product of Production Example 1 2.0 L-Ascorbic Acid 2-Glucoside 2.0 Tranexamic acid 2.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Glycerin 5.0 1,3-butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total volume 100 parts)

[0074] Prescription example 13. Emulsion [Ingredients] Part Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Fermented product of Production Example 1 2.0 Ascorbic acid phosphate magnesium salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Sodium hyaluronate 1.0 Purified water (enough to make the total volume 100 parts)

[0075] Prescription example 14. Emulsion An emulsion was obtained with the same composition as in Formulation Example 13, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

[0076] Prescription example 15. Emulsion An emulsion was obtained with the same composition as in Formulation Example 13, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide.

[0077] Prescription example 16. Emulsion An emulsion was obtained with the same composition as in Formulation Example 13, except that 3.0 parts of nicotinamide were used in place of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide.

[0078] Prescription example 17. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Shea Butter 2.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Fermented product of Production Example 1 2.0 Lactic acid bacteria fermented rice 3.0 Hydrogenated Lecithin 2.0 Eucheuma muscaria extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 1,3-butylene glycol 5.0 Methylparaben 0.1 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0079] Prescription example 18. Wrinkle improvement cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Di(octyldodecyl / phytosteryl / behenyl) lauroyl glutamate 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 Nicotinamide 5.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Fermented product of Production Example 1 2.0 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Oil-soluble Panax ginseng extract 2.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Panax ginseng extract 0.5 Fermented ginseng extract 0.5 Hydrolyzed rice extract 0.5 Cherry Blossom Leaf Extract 0.5 Taiso Extract 0.5 Tea leaf extract 0.5 Centella asiatica extract 0.5 Purified water (enough to make the total volume 100 parts)

[0080] Prescription example 19. Cream A cream was obtained with the same composition as in Formulation Example 18, except that 3.0 parts of pantothenyl alcohol was used in place of 5.0 parts of nicotinamide.

[0081] Prescription example 19. Cream A cream was obtained with the same composition as in Formulation Example 18, except that 3.0 parts of dl-α-tocopherol acetate was used in place of 5.0 parts of nicotinamide.

[0082] Prescription example 20. Cream A cream was obtained with the same composition as in Formulation Example 18, except that 2.0 parts of L-ascorbic acid 2-glucoside was used in place of 5.0 parts of nicotinamide.

[0083] Example 21. Pack [Ingredients] Part Dipropylene Glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetyl alcohol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.3 Ammonium glycyrrhizinate 0.3 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Fermented product of Production Example 1 1.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total volume 100 parts)

[0084] Prescription example 22. Hair shampoo [Ingredients] Part Sodium Laureth Sulfate 10.0 Glyceryl Monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl Alcohol 2.0 Behenyl Alcohol 2.0 Dimethicone 3.0 Fermented product of Production Example 1 2.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene Glycol 2.0 Purified water (enough to make the total volume 100 parts)

[0085] Example 23. Hair conditioner [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetyl alcohol 3.0 Stearyl Alcohol 1.0 Fermented product of Production Example 1 2.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water (enough to make the total volume 100 parts)

[0086] Formulation example 24. Cleansing cosmetics [Ingredients] Part Potassium cocoyl glycinate 5.0 Glycerin 10.0 Glyceryl Caprylate 1.0 Sodium lauroyl aspartate 10.0 Fermented product of Production Example 1 1.0 Cetyl alcohol 3.0 Myristyl Alcohol 3.0 Isopropyl methyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated Benzalkonium Chloride Solution 50 0.2 Benzalkonium chloride 0.1 Purified water (enough to make the total volume 100 parts)

[0087] Prescription example 25. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Fermented product of Production Example 1 2.0 Glycerin 3.0 1,3-butylene glycol 2.0 L-Ascorbic Acid 2-Glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Eelgrass extract 1.0 Rice extract hydrolyzate 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0088] Prescription example 26. Beauty serum [Ingredients] Part 1,3-butylene glycol 5.0 Xanthan gum 1.0 Lactic acid bacteria culture 1.0 Fermented product of Production Example 1 2.0 Eggplant extract 1.0 Tea Leaf Extract 1.0 Sweet potato shochu lees extract 1.0 Citric acid 0.3 Sodium citrate 0.6 Purified water (enough to make the total volume 100 parts)

[0089] Prescription Example 27. Beverages Fermented product of Production Example 1 10.0 Citric acid 0.1 Glucoside Ceramide 0.1 Tremella fuciformis polysaccharide 0.1 Longan extract 0.1 Guava Leaf Extract 0.1 Vitamin C 0.01 Purified water (enough to make the total volume 100 parts)

[0090] Prescription Example 28. Beverages Fermented product of Production Example 1 10.0 Proteoglycan 1.0 Water hyacinth extract 1.0 Rice bran extract 1.0 Ginger extract 1.0 Yuzu Seed Extract 1.0 Panax ginseng extract 0.1 Purified water (enough to make the total volume 100 parts)

[0091] Prescription Example 29. Beverages A beverage was obtained with the same composition as in Formulation Example 28, except that 10.0 parts of the fermented product of Production Example 7 was used instead of 10.0 parts of the fermented product of Production Example 1.

Claims

1. An external skin preparation containing, as an active ingredient, a collagen fermentation product produced by a microorganism belonging to the Labyrinthulea class, Streptomyces genus, or Cyanobacteria, which has one or more enzyme activities selected from the group consisting of protease activity and peptidase activity.

2. A food or beverage containing, as an active ingredient, a collagen fermentation product obtained by a microorganism belonging to the Labyrinthulea class, Streptomyces genus, or Cyanobacteria, which has one or more enzyme activities selected from the group consisting of protease activity and peptidase activity.

3. A method for producing a fermented product, comprising a step of fermenting collagen with a microorganism of the class Labyrinthulea, actinomycetes (genus Streptomyces), or cyanobacteria, which has one or more enzyme activities selected from the group consisting of protease activity and peptidase activity.

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