topical skin preparations

Abstract

To provide a topical skin preparation that is made from a natural product and has high biological safety, and is used to improve the trouble of pores.SOLUTION: The present invention provides a topical skin preparation for pore improvement, containing a fermented plant of Hibiscus (Malvaceae) and plant extract of Rosa (Rosaceae) as active ingredients.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation for improving pores, which contains as active ingredients a combination of extracts of plants belonging to the genus Rosa in the family Rosaceae and fermented products of plants belonging to the genus Hibiscus in the family Malvaceae. [Background technology]

[0002] It has been known that the problem of pores on the face, arms, legs, etc. is the excessive accumulation of sebum and dead skin cells, which causes noticeable darkening. Although darkening of pores can sometimes be improved by washing it away with cleansing cosmetics, there is also the problem that pore problems can be caused by factors such as deformation of pores due to sagging skin, and therefore cleansing cosmetics alone cannot sufficiently improve pore problems. DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0003] In view of the problems of the conventional technology, the present inventors have newly discovered that a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant belonging to the genus Rosaceae has an excellent synergistic effect in improving pores.

[0004] Conventionally, topical skin preparations containing as an active ingredient a fermented product of a plant belonging to the genus Hibiscus of the family Malvaceae are known, for example, from Patent Document 1, and topical skin preparations containing as an active ingredient an extract of a rose belonging to the genus Rosa of the family Rosaceae are known, for example, from Patent Documents 2 to 6.

[0005] [Patent Document 1] Patent Publication No. 2006-347925 [Patent Document 2] Patent Publication No. 2000-327555 [Patent Document 3] JP 2001-064192 [Patent Document 4] JP 2001-163794 [Patent Document 5] JP 2001-316277 A [Patent Document 6] Patent Publication No. 2014-240375

[0006] However, it was not known that a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant belonging to the genus Rosaceae has a synergistic effect of improving pores. [Means for solving the problem]

[0007] The present invention relates to an external skin preparation for improving pores, which contains a fermented product of a plant belonging to the genus Hibiscus of the family Malvaceae and an extract of a plant of the genus Rosa of the family Rosaceae. [Effects of the Invention]

[0008] The present invention provides an external skin preparation that is highly safe and effective for the body and exhibits a synergistic effect in improving pores, by using as active ingredients a combination of a fermented product of a plant belonging to the genus Hibiscus in the family Malvaceae and an extract of a plant of the genus Rosaceae. [Brief explanation of the drawings]

[0009] [Figure 1] FIG. 1 is a diagram showing the evaluation results of pore improvement. [Figure 2] FIG. 1 is a diagram showing the evaluation results of pore improvement. DETAILED DESCRIPTION OF THE INVENTION

[0010] Preferred embodiments of the present invention will now be described in detail. First, we will explain the fermented products of plants belonging to the genus Hibiscus in the family Malvaceae. Examples of plants in the genus Hibiscus include roselle (Hibiscus sabdariffa L.), rose of sharon (Hibiscus syriacus), Hibiscus mutabills, Hibiscus coccineus, Hamamou (Hibiscus tiliaceus), Hibiscus rosa-sinensis, and Hibiscus schizopetalus. There are no particular limitations on the parts of Hibiscus plants that can be used, and any appropriate part can be used, such as the whole plant, leaves, stems, flowers, calyx, stamens, pistils, stems, roots, seeds, or fruits, but the use of the whole plant, flowers, and calyx is preferred.

[0011] As a source of nutrients for fermentation of plants of the genus Hibiscus, the plant itself (hereinafter referred to as the plant body) may be used, or an extract obtained from the plant body by the solvent extraction method described below may be used. When an extract is used, it is also possible to carry out fermentation with the plant body as it is, without removing the plant body from the extract by solid-liquid separation. Here, the plant may be fresh, or may be pre-dried or semi-dried. Furthermore, the plant may be used as it is after harvesting.

[0012] In the present invention, examples of microorganisms used in the fermentation of plants of the genus Hibiscus include lactic acid bacteria, bifidobacteria, koji mold, natto bacteria, tempeh bacteria, yeast, etc., and generally one or more species selected from each of these species are used, but in some cases, bacteria belonging to different species may be used in combination as long as they do not interfere with each other's fermentation.

[0013] For example, lactic acid bacteria include Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbrueckii, and other Lactobacillus species; Carnobacterium divergens, Carnobacterium piscicola, and other Carnobacterium species; Leuconostoc mesenteroides, Leuconostoc citreum, and other Leuconostoc species; Streptococcus faecalis, Streptococcus faecalis, and other Lactobacillus species; Lactic acid bacteria of the genus Streptococcus, such as Streptococcus faecalis and Streptococcus pyogenes; lactic acid bacteria of the genus Enterococcus, such as Enterococcus caseliflavus and Enterococcus sulfreus; lactic acid bacteria of the genus Lactococcus, such as Lactococcus plantarum and Lactococcus rafinolactis; lactic acid bacteria of the genus Veissella, such as Weissella confusa and Weissella kandleri; Atopobium minutum and Atopobium Lactic acid bacteria of the genus Atopobium, such as Atopobium parvulus; lactic acid bacteria of the genus Vagococcus, such as Vagococcus fluvialis and Vagococcus salmoninarum;Examples include lactic acid bacteria of the genus Pediococcus, such as Pediococcus damnosus and Pediococcus pentosaceus, and marine lactic acid bacteria, such as Marinilactobacillus phychrotolerans.

[0014] For example, bifidobacteria include Bifidobacterium bifidum and Bifidobacterium breve, and any strain classified as bifidobacteria can be used.

[0015] Examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus.

[0016] Examples of natto bacteria include bacteria of the genus Bacillus, such as Bacillus natto, Bacillus subtilis, and Bacillus circulans. Among these, Bacillus natto is most preferred because it is widely used in foods and is highly safe.

[0017] Examples of tempeh fungi include fungi (molds) of the genus Rhizopus, such as Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus niveus, and Rhizopus oryzae.

[0018] Examples of yeast include yeasts of the genus Saccharomyces such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus; yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, and Torulaspora rosei; yeasts of the genus Torulaspora such as Zygosaccharomyces rouxii and Zygosaccharomyces sojae; Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces soybean, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; yeasts of the genus Candida, such as Aureobasidium pullulans, Aureobasidium mansonii, and Aureobasidium microstictum. Examples include yeasts of the genus Aureobasidium such as Aureobasidium microstictum.Of the above-mentioned yeasts, Saccharomyces cerevisiae is preferred from the viewpoints of safety and effectiveness, but Saccharomyces cerevisiae of any origin can be used, including those derived from plants such as sake, lilies, and cherry blossoms, and those of marine origin.

[0019] When the above-mentioned suspension or extract is fermented with microorganisms, sterilization is carried out before the fermentation step to remove unwanted bacteria that may hinder fermentation. This method of sterilizing and removing unwanted bacteria may involve washing the fermentation material with sterilizing ethanol or the like beforehand and then suspending it in a sterile solvent such as sterile water. Alternatively, the fermentation material may be suspended in a solvent and then sterilized by heat sterilization or the like. Commonly used heat sterilization methods include autoclave sterilization, in which the suspension is heated to 120-130°C for 10-20 minutes, and intermittent sterilization, in which the suspension is kept at 80-90°C for 60-120 minutes, repeated once a day for 2-3 days.

[0020] The sterilized suspension is placed in a fermentation tank, and the microorganisms are inoculated and fermented. The inoculation amount of the microorganisms is 10 7 ~10 8 If the inoculum amount is greater than the above range, the fermentation time will not change significantly, while if it is less than the above range, it will take a long time to complete the fermentation, which is not desirable.

[0021] A preferred example of a method for fermenting the above-mentioned plants using the above-mentioned microorganisms is as follows. First, the fermentation material of the plant is soaked or suspended in a fermentation medium to prepare a suspension for fermentation. In this case, the plant may be used fresh or may be dried or semi-dried in advance. Furthermore, the plant may be used as is after harvesting, but the fermentation efficiency can be improved by shredding or crushing it into fine particles.

[0022] The fermentation medium for suspending the fermentation material may be water or a mixture of water with lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol (propanediol), 1,3-butylene glycol, glycerin, etc.). Sugars such as glucose, fructose, and sucrose may also be added to these media. However, it is most preferable to use water alone, as this is the medium in which microorganisms are most likely to exert their effects and to avoid the production of fermentation by-products due to the presence of nutritive substances other than those from the plant that is the fermentation material.

[0023] Before subjecting this suspension of fermentation material to the fermentation process, it is sterilized to remove unwanted bacteria that may hinder fermentation. In this case, the sterilization method may involve washing and sterilizing the fermentation material in advance with sterilizing ethanol or the like, and then suspending it in a sterile medium such as sterile water. Alternatively, the fermentation material may be suspended in a medium and then heat-sterilized. Commonly used heat-sterilization methods include autoclave sterilization, in which the suspension is heated at 120-130°C for 10-20 minutes, and intermittent sterilization, in which the suspension is kept at 80-90°C for 60-120 minutes, and this is repeated once a day for 2-3 days.

[0024] Next, this sterilized suspension is placed in a fermentation tank, and the microorganisms are inoculated therein to carry out the fermentation treatment. 7 ~10 8 If the inoculum amount is greater than the above range, the fermentation time will not change significantly, while if it is less than the above range, it will take a long time to complete the fermentation, which is not desirable.

[0025] The fermentation temperature is generally in the range of 5 to 50°C. The fermentation period is generally 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation period is shorter than the above-mentioned general range, the fermentation does not proceed sufficiently and the effectiveness of the fermented product tends to decrease. On the other hand, if the fermentation period is longer than 10 days, not only will no further increase in effectiveness be observed, but coloring and an increased fermentation odor will occur, both of which are undesirable.

[0026] In carrying out the above fermentation treatment, in order to enable the microorganisms to more effectively utilize the plant components, an enzyme may be added to the suspension before or during inoculation of the microorganisms, or in some cases during fermentation after inoculation, to subject the plant, which is the fermentation material, to enzymatic hydrolysis treatment. In this case, as described above, the enzyme that can be used is at least one enzyme selected from protease, carbohydrate-degrading enzyme, pectin-degrading enzyme, and lipase.

[0027] Regarding the treatment conditions such as pH, temperature, and time, if the enzymatic treatment is carried out before fermentation, it is preferable to carry out the treatment for 1 to 24 hours at approximately the optimum pH and temperature for the enzyme used. On the other hand, if the treatment is carried out in parallel with fermentation, the same conditions as those for the fermentation may be used.

[0028] After the above fermentation process is complete, the fermented broth is subjected to heat sterilization at 80-100°C for approximately 10-120 minutes to sterilize the microorganisms and, if an enzyme treatment is used in combination, to inactivate the enzymes. After sterilization, the fermented broth is used as is, or the liquid phase is separated by solid-liquid separation, typically and preferably by filtration or centrifugation, and, if necessary, the pH is adjusted to a pH of 4-9, which is the pH range for typical cosmetics. If necessary, the liquid phase may be diluted or concentrated to an appropriate concentration and used as a cosmetic ingredient. In some cases, the liquid phase after solid-liquid separation may be solidified by conventional methods such as spray drying or freeze drying, and then, if necessary, pulverized into a powder.

[0029] Next, we will explain extracts from plants of the genus Rosaceae. The plants used as the extract material in the present invention may be any variety (including hybrids and subspecies). Examples include Rosa Centifolia, Rosa Damascena, Rosa gallica, Rosa moschata, and Rosa alba.

[0030] The material used in the present invention may be any of the whole plant, leaves, flowers, stems, seeds, fruits, roots, placenta, etc. of the Rosa plant, but it is preferable to use the whole plant or flowers.

[0031] The extract is prepared by first washing a plant of the genus Rosa (for example, the whole plant or inflorescences) with water if necessary to remove foreign matter, then either leaving it as is or drying it, and then shredding or pulverizing it as necessary, and contacting it with an extraction solvent to carry out extraction. Extraction can be carried out by contacting it with an extraction solvent according to a conventional method such as immersion, but supercritical extraction can also be used instead of immersion.

[0032] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination.

[0033] Among these extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of skin irritation and efficacy, and also because they can be widely applied to cosmetics. Preferred examples of hydrophilic solvents include the use of water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or a mixed solvent of water and a lower alcohol (especially ethanol), or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin), among which water alone or a mixed solvent of water and 1,3-butylene glycol is particularly preferred.

[0034] When a mixed solvent is used, the mixing ratio is preferably in the range of 1:10 to 20:1 by volume (same applies hereinafter) for a mixed solvent of water and 1,3-butylene glycol, 1:10 to 25:1 for a mixed solvent of water and ethanol, and 1:10 to 20:1 for a mixed solvent of water and glycerin.

[0035] The weight ratio of the dried part of the damask rose (for example, the whole plant or flower parts) to the extraction solvent is preferably in the range of 1:1 to 1:50.

[0036] When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0037] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used, but for example, when water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0° C. to 80° C. The extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week).

[0038] If necessary, the extract of a plant of the genus Rosa may be subjected to a hydrolysis treatment prior to or in parallel with the extraction treatment of the present invention, which may improve the storage stability of the extract of a plant of the genus Rosa and allow the extract to be used more effectively as an ingredient in cosmetic compositions.

[0039] When the extract is subjected to enzymatic hydrolysis, the enzymes used may be one or more selected from the following enzyme group: proteolytic enzymes such as actinase, papain, and pepsin; amylolytic enzymes such as glucoamylase, α-amylase, and β-amylase; cellulose-degrading enzymes such as cellulase, hemicellulase, and pectinase; and lipolytic enzymes such as lipase. However, it is more preferable to use a combination of one or more enzymes selected from each of these enzyme groups.

[0040] The extract and fermented product prepared as described above may be used as a cosmetic ingredient as is, generally after adjusting the pH to 3 to 8, or may be used at the desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0041] Uses of topical skin preparations containing the extracts and fermented products of the present invention include, but are not limited to, topical skin preparations (topical medicinal components, topical quasi-drugs, and cosmetics), such as emulsions, creams, lotions, essences, gels, packs, sheet masks, and slimming agents.

[0042] The amount of the active ingredient according to the present invention in external skin preparations (cosmetics and quasi-drugs) is generally in the range of 0.0002 to 1.0 wt % (solids weight %, the same applies below) in terms of solid content in the case of basic cosmetics, and preferably in the range of 0.002 to 0.2 wt %.

[0043] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples of suitable glycerides include hydrocarbons such as cocos and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0044] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0045] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0046] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, various amino acids and their derivatives.

[0047] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0048] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0049] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. Examples include chrysoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0050] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0051] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0052] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0053] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0054] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0055] Further, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0056] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzates thereof.

[0057] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc.; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is hexanoyl, octanoyl, decanoyl, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc.; L-ascorbic acid tetrafatty acid esters such as 3-isopalmitate, ... -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), and tranexamic acid amides (e.g., tranexamic acid methylamide). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0058] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0059] Production Example 1. Preparation of fermented Hibiscus plants (1) A suspension was made by adding 950g of purified water to 50g of dried flowers and calyx of a plant (Roselle) of the genus Hibiscus. The suspension was then neutralized with alkali to a pH of around 6, and then sterilized by heating at 80-90°C for 1 hour. 100g of lactic acid bacteria (Lactobacillus plantarum) were added to the sterilized suspension. 8 The cells / mL were inoculated and statically cultured for 3 days at 37° C. After the culture was completed, the culture solution was heat sterilized and then filtered to obtain 737 g of a lactic acid bacteria fermentation solution (solid concentration 3.32%).

[0060] Production Example 2. Preparation of fermented Hibiscus plants (2) The same procedure as in Production Example 1 was carried out except that the flowers and calyx of Rose of Sharon (Hibiscus syriacus) were used instead of roselle, to obtain 650 g of a lactic acid bacteria fermentation product solution (solid concentration 2.54%).

[0061] Production Example 3. Preparation of fermented Hibiscus plants (3) The same procedure as in Production Example 1 was carried out except that yeast Saccharomyces cerevisiae was used instead of lactic acid bacteria, to obtain 805 g of a yeast fermentation product solution (solid concentration 3.18%).

[0062] Preparation Example 4. Preparation of Rosa plant extract solution (1) Petals of Rosa Damascena (Rosaceae family) were dried, and 225g of purified water was added to 15g of the dried material, and the mixture was soaked at 4℃, followed by the addition of 225g of 1,3-butylene glycol. Extraction was carried out at 4℃, and the mixture was filtered, yielding 390g of a brown, transparent flower extract solution (solid concentration 1.07%).

[0063] Preparation Example 5. Preparation of Rosa plant extract solution (2) 398 g of a brown, transparent flower extract solution (solid concentration 1.10%) was obtained by the same process as in Preparation Example 1, except that Rosa Centifolia petals were used instead of the Rosa Damascena petals used in Preparation Example 4.

[0064] Preparation Example 6: Preparation of Rosa plant extract solution (3) 380 g of a brown, transparent flower extract solution (solid concentration 1.03%) was obtained by the same process as in Production Example 1, except that Rosa gallica petals were used instead of the Rosa Damascena petals used in Production Example 4.

[0065] Example 1. Preparation of the composition A combination of an extract of a plant of the genus Ros and a fermented product of a plant of the genus Hibiscus can be prepared, for example, by mixing the fermented product of any one of Production Examples 1 to 3 with the extract of any one of Production Examples 4 to 6. The mixing ratio, in terms of solid content, of the extract of a plant of the genus Ros to the fermented product of a plant of the genus Hibiscus is preferably 1:10 to 10:1, more preferably 1:3 to 3:1. For example, the compositions shown in Table 1 can be prepared.

[0066] [Table 1] JPEG0007795761000001.jpg142137

[0067] Test example 1. Pore improvement evaluation test (1) Sample preparation A lotion (sample of the present invention) containing a composition according to the present invention (composition 10) and a comparative sample were prepared as follows. Note that the percentages below indicate the weight percentages in the lotion. (A) Sample of the present invention Composition 10 (0.6%), 1,3-butylene glycol (0.18%), ethanol (5.0%), paraben (0.2%), water (94.02%) (B) Comparative sample 1,3-butylene glycol (0.18%), ethanol (5.0%), paraben (0.2%), water (94.62%) (2) Evaluation test Test areas were set on the outer sides of both forearms of five subjects. The initial skin shape, including pores, before the test was transferred to a replica using a replica creation kit (Nippon Ash). An appropriate amount of the inventive sample (lotion) was applied to the test area of ​​one arm, and a comparative sample (lotion) was applied to the other arm twice a day. After one month, the skin shape was transferred to a replica in the same manner as the initial state. The area and volume of the convex parts (corresponding to pores in the skin) of the obtained replicas were determined by two-dimensional image analysis using oblique illumination. The evaluation results were expressed as the average value relative to the initial value, which was set to 100.

[0068] The results of Test Example 1 are shown in Figures 1 and 2. As shown in Figures 1 and 2, it was confirmed that the area ratio and volume ratio of pores were reduced in the test area where the sample of the present invention was applied, while the area ratio and volume ratio of pores were increased in the test area where the comparative sample was applied. From these results, it was confirmed that the composition of the present invention has the effect of significantly reducing pores and reducing the visibility of pores, that is, has a pore improvement effect. Furthermore, similar effects are expected for Compositions 1 to 9 and 11 to 15.

[0069] Prescription example 1. Lotion [Ingredients] Part Eucalyptus oil 0.2 Polyoxyethylene (5.5) Cetyl Alcohol 0.4 Composition 10 1.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Stearyl glycyrrhizinate 0.05 Isopropylmethylphenol 0.1 Align In 0.1 D-Pantothenyl alcohol 0.1 Salicylic acid 0.5 Urea 5.0 l-menthol 0.9 dl-menthol 0.2 1,3-butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Hinokitiol 0·003 Photosensor No. 201 0.002 Purified water (enough to make the total volume 100 parts)

[0070] Prescription example 2: Lotion [Ingredients] Part Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Cetyl alcohol 2.0 Behenyl Alcohol 2.0 Methylparaben 0.1 Composition 4 1.0 Ascorbic Acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc Oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-butylene glycol 5.0 Potassium hydroxide 0.5 Purified water (enough to make the total volume 100 parts)

[0071] Prescription example 3: Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 5 was used instead of composition 4 contained in Formulation Example 2.

[0072] Prescription example 4: Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 6 was used instead of composition 4 contained in Formulation Example 2.

[0073] Prescription example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 7 was used instead of composition 4 contained in Formulation Example 2.

[0074] Prescription example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 8 was used instead of composition 4 contained in Formulation Example 2.

[0075] Prescription example 7. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 9 was used instead of composition 4 contained in Formulation Example 2.

[0076] Prescription example 7. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 10 was used instead of composition 4 contained in Formulation Example 2.

[0077] Prescription example 8. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 11 was used instead of composition 4 contained in Formulation Example 2.

[0078] Prescription example 9. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that composition 12 was used instead of composition 4 contained in Formulation Example 2.

[0079] Prescription example 10. Lotion [Ingredients] Part Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Composition 4 1.0 Ascorbic Acid Glucoside 2.0 Tranexamic acid 2.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Glycerin 5.0 1,3-butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total volume 100 parts)

[0080] Prescription example 11. Emulsion [Ingredients] Part Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Composition 7 1.0 Ascorbic acid phosphate magnesium salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Sodium hyaluronate 1.0 Purified water (enough to make the total volume 100 parts)

[0081] Prescription example 12. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 10 was used instead of composition 7.

[0082] Prescription example 13. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 11 was used instead of composition 7.

[0083] Prescription example 14. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that composition 12 was used in place of composition 7.

[0084] Prescription example 15. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

[0085] Prescription example 16. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide.

[0086] Prescription example 17. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 3.0 parts of nicotinamide was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

[0087] Prescription example 18. Cream [Ingredients] Part Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Di(octyldodecyl / phytosteryl / behenyl) lauroyl glutamate 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Composition 10 2.0 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Oil-soluble Panax ginseng extract 2.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Purified water (enough to make the total volume 100 parts)

[0088] Example 19. Pack [Ingredients] Part Dipropylene Glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetyl alcohol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Composition 10 2.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total volume 100 parts)

[0089] Prescription example 20. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Composition 7 2.0 Glycerin 3.0 1,3-butylene glycol 2.0 L-Ascorbic Acid 2-Glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Eelgrass extract 1.0 Rice extract hydrolyzate 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0090] Prescription example 21. Beauty serum [Ingredients] Part Ethanol 2.0 Glycerin 5.0 1,3-butylene glycol 5.0 Methylparaben 0.1 Composition 1 2.0 Citric acid 0.3 Sodium citrate 0.6 Purified water (enough to make the total volume 100 parts)

[0091] Prescription example 22. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that Composition 2 was used instead of Composition 1 in the beauty serum of Formulation Example 21.

[0092] Prescription example 23. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 3 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0093] Prescription example 24. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 13 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0094] Prescription example 25. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 14 was used instead of composition 1 in the beauty serum of Formulation Example 21.

[0095] Prescription example 26. Beauty serum A beauty serum was obtained in the same manner as in Formulation Example 21, except that composition 15 was used instead of composition 1 in the beauty serum of Formulation Example 21.

Claims

[Claim 1] A topical skin preparation for reducing the appearance of pores, comprising a fermented product of roselle (Hibiscus sabdariffa L.) or rose of sharon (Hibiscus syriacus) belonging to the genus Hibiscus of the family Malvaceae, using lactic acid bacteria or yeast, and an extract of Rosa Damascena, Rosa Centifolia, or Rosa gallica belonging to the genus Rosa of the family Rosaceae, using a mixed solvent of water and polyhydric alcohol.

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