Cosmetic composition, screening method and cosmetic method

Abstract

The present invention aims to provide a novel cosmetic composition that is capable of sufficiently improving skin conditions comparable to IPL irradiation in terms of skin whitening, anti-aging, etc., and that can be easily used by many users, as well as a novel cosmetic method using the same. The present invention relates to a cosmetic composition containing an IPL irradiation-mimicking agent. The cosmetic composition of the present invention exhibits effects comparable to those of phototherapy and can be used as an alternative to IPL irradiation. Furthermore, in the cosmetic composition of the present invention, the IPL irradiation-mimicking agent preferably contains at least a plant extract containing a flavonoid or a seaweed extract.

Description

[Technical Field]

[0001] The present invention relates to a cosmetic composition, a screening method, a cosmetic method, and an IPL radiation-like agent. [Background technology]

[0002] In recent years, phototherapy has become increasingly popular in the field of aesthetic medicine. One new treatment that has attracted attention is Intense Pulse Light (IPL), also known by device names such as "Photofacial (registered trademark)," "LimeLight," and "Lumenis One." IPL uses light with a wide wavelength range, eliminating short wavelengths that are harmful to the skin, such as ultraviolet light. Specifically, it is light with multiple wavelengths in the 500-1200 nm wavelength range, including a broad range of visible and infrared light. Irradiating multiple wavelengths in this way can effectively improve a variety of conditions. By using filters to limit the wavelength range and waveform of these wavelengths, irradiation can be tailored to the skin condition. This treatment is also effective against loss of firmness, enlarged pores, uneven skin tone, and dullness, and is said to be effective in improving aging symptoms across the entire face. Because the IPL treatment mentioned above contains light of multiple wavelengths, it is believed that by irradiating the entire face, rather than just targeting spots such as blemishes or moles as with conventional laser treatments, it can effectively improve the multiple problems mentioned above.

[0003] However, because there are only a limited number of medical institutions that can provide phototherapy such as IPL irradiation, because continuous or continuous treatment is recommended depending on the skin condition being treated, and because some people have skin conditions or characteristics that are not suitable for treatment, not everyone can easily undergo the treatment. However, the demand for skin whitening, anti-aging, etc. is increasing every day, and there is a demand for options tailored to individual lifestyles, skin conditions, skin characteristics, etc. In addition, although phototherapy is highly effective, its mechanism of action has remained largely unknown. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2008-11994 [Non-patent literature]

[0005] [Non-Patent Document 1] Skill Up Skin Laser Treatment (edited by Akira Kawada), Chugai Medical Publishing (published in 2011), pp.176-190 Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention was made in light of these circumstances, and aims to clarify the mechanism of action of phototherapy and provide a novel cosmetic composition that is as effective in improving skin condition as IPL irradiation in terms of skin whitening, anti-aging, etc., and that can be easily used by many users, as well as a new cosmetic method using the same. [Means for solving the problem]

[0007] As a result of extensive research aimed at solving the above-mentioned problems, the inventors have found that IPL irradiation of epidermal cells suppresses the expression of genes involved in melanin synthesis and melanosome transport and promotes the expression of genes involved in cell turnover. In other words, the whitening mechanism of IPL irradiation is thought to be through its action on the genes involved in melanin synthesis, melanosome transport, and cell turnover. By utilizing this finding, the inventors have conceived the possibility of developing topical compositions such as cosmetics that can achieve the same effects as IPL irradiation, i.e., IPL-inspired products. Based on this finding, they searched for substances that can suppress the expression of these genes, whose expression is suppressed by IPL irradiation, in a manner similar to IPL irradiation. Among many substances, they found that plant extracts and seaweed extracts containing flavonoids exhibit effects similar to those of IPL irradiation. The gist of the present invention is as follows.

[0008] [1] A cosmetic composition containing an IPL radiation-like agent. [2] The cosmetic composition according to [1], wherein the IPL radiation-like agent contains at least a plant extract containing a flavonoid as an active ingredient. [3] The cosmetic composition according to [2], wherein the plant extract is an extract of at least one plant selected from the group consisting of Cucurbitaceae, Asteraceae, Fabaceae, Rosaceae, Salicaceae, Meliaceae, Rutaceae, Saxifragaceae, Plantaginaceae, Passifloaceae, Cannabaceae, Monimiaceae, Aesculaceae, Ericaceae, Paeoniaceae, Lamiaceae, Malvaceae, and Zingiberaceae. [4] The cosmetic composition according to [1], wherein the IPL radiation-like agent contains at least a seaweed extract as an active ingredient. [5] A cosmetic composition according to any one of [1] to [4], wherein the IPL radiation-like agent regulates the expression of melanin-related molecules in skin cells. [6] The melanin-related molecules are OCA2, SLC45A2, and RAB27. A The cosmetic composition according to [5], wherein the cosmetic composition is at least one selected from the group consisting of: [7] The cosmetic composition according to any one of [2], [3], [5] and [6], wherein the plant extract is at least one selected from the group consisting of Jiaogulan extract, artichoke extract, licorice root extract, Rosa multiflora fruit extract, White willow bark extract, Melia azadirachta leaf extract, Mandarin orange peel extract, Saxifraga extract, Plantago major seed extract, Passiflora edulis fruit extract, Hops flower extract, Tambourissa trichophylla leaf extract, Rosemary extract, Burdock extract and Althaea extract. [8] The cosmetic composition according to any one of [1] to [7], further comprising at least one selected from the group consisting of a turnover promoter (excluding the above-mentioned IPL irradiation-like agent), an anti-glycation component, and a whitening active component. [9] A cosmetic composition according to any one of [1] to [8], which is used as a skin tone improver.

[10] A method for screening ingredients for improving skin tone, characterized by screening ingredients that are effective in improving skin tone using genes whose expression changes when skin cells are irradiated with IPL as an indicator.

[11] The screening method according to

[10] , comprising at least the steps of: allowing a test substance to act on cultured skin cells; and measuring the gene expression in the cultured skin cells.

[12] A cosmetic method for improving and enhancing skin condition, characterized by using an IPL radiation-like agent.

[13] An IPL irradiation-like agent containing, as an active ingredient, at least one plant extract selected from the group consisting of Jiaogulan extract, artichoke extract, licorice root extract, multiflora rose fruit extract, white willow bark extract, Melia azadirachta leaf extract, mandarin orange peel extract, saxifrage extract, Plantain asiatica seed extract, Passiflora edulis fruit extract, Hops flower extract, Tambourissa trichophylla leaf extract, rosemary extract, burdock extract, and Althaea officinalis extract.

[14] An IPL irradiation-like agent containing, as an active ingredient, an extract of at least one seaweed selected from the group consisting of brown algae, red algae, green algae, and blue-green algae. [Effects of the Invention]

[0009] According to the present invention, it is possible to provide a novel cosmetic composition that can be easily used by many users and that sufficiently improves skin condition in terms of skin whitening, anti-aging, etc., similar to IPL irradiation, and also to provide a new cosmetic method using the same. DETAILED DESCRIPTION OF THE INVENTION

[0010] The present invention will be described in detail below. The terms used in this specification are to be interpreted in the sense commonly used in the art unless otherwise specified.

[0011] <Beauty composition> The cosmetic composition of the present invention contains an IPL irradiation-like agent. Here, the IPL irradiation-like agent refers to a composition that can provide the same effect as when IPL is irradiated to a subject. This composition can be used as an alternative to phototherapy such as IPL irradiation, i.e., a cosmetic composition that can be used as an alternative to IPL irradiation. The IPL irradiation-like agent may also provide some of the effects obtained by irradiating a subject with IPL. The cosmetic composition of the present invention is effective even when used alone, but by combining it with phototherapy such as IPL irradiation, it is possible to improve the effects of IPL irradiation and make them last longer.

[0012] The effects of irradiating a subject with IPL include, for example, regulating the expression of melanin-related molecules in skin cells, whitening the entire face, and improving skin tone. Also included are effects on improving aging symptoms such as dullness, lack of firmness, visible pores, uneven skin tone, and dryness (improving skin tone). Examples of the melanin-related molecules (genes and / or proteins) include OCA2, SLC45A2, and RAB27. A , HB-EGF, etc. Among these molecules, OCA2, SLC45A2, RAB27, etc. are expressed by IPL irradiation. AThe expression of these molecules is regulated in a suppressive direction. Furthermore, among these molecules, the expression of HB-EGF is regulated in a promoting direction. That is, the cosmetic composition of the present invention is capable of inhibiting the expression of OCA2, SLC45A2, and RAB27. A The cosmetic composition of the present invention can also be said to be a cosmetic composition for suppressing the expression of at least one gene selected from the group consisting of: or a cosmetic composition for promoting the expression of the HB-EGF gene. Furthermore, since the cosmetic composition of the present invention suppresses or promotes the expression of the above-mentioned genes, it can also be said to be a cosmetic composition for improving and / or enhancing skin tone, similar to IPL irradiation.

[0013] In addition to the IPL radiation-mimetic agent, the cosmetic composition of the present invention may contain other optional ingredients within the scope that does not impair the effects of the present invention. The IPL radiation-mimetic agent and other optional ingredients are described below.

[0014] [IPL radiation-like agent] In the cosmetic composition of the present invention, the IPL irradiation-like agent is not particularly limited as long as it can provide the same effect as IPL irradiation to a subject, but examples thereof include those containing various plant extracts as active ingredients, and among these, those containing at least a plant extract containing flavonoids are preferred. Also preferred are those containing at least a seaweed extract.

[0015] Examples of plant extracts containing flavonoids include extracts of plants from the Cucurbitaceae, Asteraceae, Fabaceae, Rosaceae, Salicaceae, Meliaceae, Rutaceae, Saxifragaceae, Plantaginaceae, Passifloaceae, Cannabaceae, Monimiaceae, Aesculaceae, Ericaceae, Paeoniaceae, Lamiaceae, Malvaceae, and Zingiberaceae families.

[0016] Examples of the plant extracts include Jiaogulan extract, artichoke extract, licorice root extract, Rosa multiflora fruit extract, White willow bark extract, Melia azadirachta leaf extract, Mandarin orange peel extract, Saxifraga extract, Plantain major seed extract, Passiflora edulis fruit extract, Hop flower extract, Tambourissa trichophylla leaf extract, Tilia cordata flower extract, hydrolyzed soybean peptide, White willow bark extract, Rosa izayoi extract, Rosemary extract, Burdock extract, and Althaea officinalis extract. Examples of parts of the plants to be extracted include leaves, flowers, fruits, roots, stems, skins, branches, fruits, peels, and seeds. For extraction, plant materials may be used as they are or may be dried. Plant materials may also be crushed before use. Commercially available plant extracts, such as those used as cosmetic ingredients, may also be used.

[0017] Examples of seaweed used in the seaweed extract include extracts of seaweeds such as brown algae (Kagome kelp, Risori kelp, Mitsuishi kelp, Laminaria japonica, Laminaria longa, and Undaria pinnatifida), red algae (Geranium globulus, etc.), green algae (Laver laver, etc.), and blue-green algae (Spirulina, etc.). For extraction, the seaweed raw material may be used as is or may be dried before use. Plant raw materials may also be crushed before use. Seaweed extracts commercially available as cosmetic ingredients may also be used. These seaweeds may be used alone or in combination of two or more. Of these, brown algae extracts are preferred, kelp extracts are more preferred, and Kagome kelp extracts are even more preferred, from the viewpoint of their excellent effect of promoting the expression of melanin-related molecules.

[0018] Of these, among melanin-related molecules, artichoke extract, passionflower fruit extract, gynostemma pentaphyllum extract, hydrolyzed soybean peptide (hydrolyzed soybean protein), licorice root extract, Rosa multiflora fruit extract, Tambourissa trichophylla leaf extract, and Tilia cordata flower extract are preferred, from the viewpoint of their particularly excellent effect of reducing the expression of the OCA2 gene, and artichoke extract, passionflower fruit extract, licorice root extract, and Rosa multiflora fruit extract are more preferred.

[0019] Among melanin-related molecules, from the viewpoint of being particularly effective in reducing the expression of the SLC45A2 gene, Gynostemma pentaphyllum extract, licorice root extract, Rosa multiflora fruit extract, artichoke extract, hydrolyzed soybean peptide, white willow bark extract, Melia azadirachta leaf extract, mandarin orange peel extract, saxifrage extract, Plantain major seed extract, and Tilia cordata flower extract are preferred, with Gynostemma pentaphyllum extract, licorice root extract, and Rosa multiflora fruit extract being more preferred.

[0020] Among melanin-related molecules, RAB27 in particular A From the viewpoint of having an excellent effect of reducing gene expression, Jiaogulan extract, artichoke extract, hydrolyzed soybean peptide, licorice root extract, Rosa multiflora fruit extract, Saxifraga extract, Plantago major seed extract, Tambourissa trichophylla leaf extract, Tilia cordata flower extract, and Rosa robur extract are preferred.

[0021] Among melanin-related molecules, OCA2, SLC45A2, and RAB27 A From the viewpoint of being highly effective in reducing the expression of two or more of the above genes, Gynostemma pentaphyllum extract, artichoke extract, hydrolyzed soybean peptide, licorice root extract, Rosa multiflora fruit extract, saxifrage extract, Plantain major seed extract, Tilia cordata flower extract, white willow bark extract, Mandarin orange peel extract, and Tambourissa trichophylla leaf extract are preferred, and from the viewpoint of being highly effective in reducing the expression of all three genes, Gynostemma pentaphyllum extract, artichoke extract, hydrolyzed soybean peptide, licorice root extract, Rosa multiflora fruit extract, saxifrage extract, Plantain major seed extract, and Tilia cordata flower extract are more preferred.

[0022] Among melanin-related molecules, rosemary extract, seaweed extract, Althea extract, passionflower fruit extract, and burdock extract are preferred from the viewpoint of their excellent effect in promoting the expression of the HB-EGF gene, and seaweed extract, Althea extract, and rosemary extract are more preferred.

[0023] In consideration of the effects of the present invention as a whole, the plant extracts in the cosmetic composition of the present invention are preferably Gynostemma pentaphyllum extract, artichoke extract, licorice root extract, Rosa multiflora fruit extract, White willow bark extract, Melia azadirachta leaf extract, Mandarin orange peel extract, Saxifraga extract, Plantago major seed extract, Passiflora edulis fruit extract, Hops flower extract, Tambourissa trichophylla leaf extract, Rosemary extract, and Althaea officinalis extract. Furthermore, the seaweed extract in the cosmetic composition of the present invention is preferably a brown algae extract, more preferably a kelp extract, and even more preferably a Kjellmannia crassifolia extract.

[0024] When the IPL radiation-mimetic agent is a plant extract, the content of the IPL radiation-mimetic agent in the cosmetic composition of the present invention is 0.0001% to 20% by weight, preferably 0.001% to 10% by weight, more preferably 0.01% to 5% by weight, and even more preferably 0.05% to 3% by weight. The cosmetic composition of the present invention may contain one or more of the plant extracts. When the cosmetic composition of the present invention contains two or more of the plant extracts, the total content of the two or more plant extracts is defined as the content of the plant extract.

[0025] Unlike conventional whitening mechanisms that rely on the inhibition of tyrosinase activity, the cosmetic method and cosmetic composition of the present invention exert the effect of improving skin tone (skin tone improving effect) by directly acting on genes involved in melanin synthesis and melanosome transport, which determine the whiteness of the skin.

[0026] Here, improving skin tone means improving age spots, freckles, and dullness and pigmentation that come with aging, bringing the skin closer to a more natural and even color (skin tone improvement), as well as improving the skin tone of skin that is naturally darker.

[0027] Examples of optional components contained in the cosmetic composition of the present invention include whitening active ingredients, turnover promoters (excluding the IPL irradiation-like agent of the present invention), anti-glycation ingredients, antioxidant ingredients, anti-aging ingredients, anti-inflammatory agents, cooling agents, disinfectants, vitamins, organic acids, moisturizing ingredients, polyhydric alcohols, scrubbing agents, UV-absorbing ingredients, UV-scattering ingredients, astringent ingredients, peptides or derivatives thereof, amino acids or derivatives thereof, cleansing ingredients, keratin softening ingredients, cell-activating ingredients, blood circulation-promoting ingredients, etc. In the cosmetic composition of the present invention, these ingredients may be used alone or in combination of two or more.

[0028] Examples of the whitening active ingredient include tocopherol, vitamin C and its derivatives, arbutin, kojic acid, placenta, ellagic acid, nicotinamide, tranexamic acid and its derivatives, hydroquinone, Rucinol (registered trademark), 4-methoxysalicylic acid potassium salt, linoleic acid and its derivatives, etc.

[0029] Examples of the turnover promoter include vitamins, keratin softening ingredients, cell activating ingredients, blood circulation promoting ingredients, etc., which will be described later.

[0030] Examples of the anti-glycation ingredients include plant extracts such as Budreja axillaris leaf extract, evening primrose oil, Amla fruit, fruit juice or extracts thereof, L-arginine, L-lysine, hydrolyzed casein, hydrolyzable tannin, carnosine, etc.

[0031] Examples of the antioxidant components include components derived from plants (e.g., grapes, ginseng, comfrey, etc.); proanthocyanidin, tocopherol and its derivatives, ascorbic acid and its derivatives, hesperidin and its derivatives, ergothioneine, sodium bisulfite, erythorbic acid and its salts, flavonoids, glutathione, etc.

[0032] Examples of the anti-aging ingredients include hydrolyzed soy protein, retinoids (retinol and its derivatives, retinoic acid, retinal, etc.), pangamic acid, kinetin, ursolic acid, turmeric extract, sphingosine derivatives, silicon, silicic acid, N-methyl-L-serine, mevalonolactone, etc.

[0033] Examples of the anti-inflammatory agents include allantoin and its derivatives, glycyrrhetinic acid and its derivatives, salicylic acid derivatives, aminocaproic acid, azulene and its derivatives, zinc oxide, and tocopherol acetate.

[0034] Examples of the above-mentioned cooling agents include terpenes such as menthol, camphor, geraniol, etc. (which may be in the d-, l-, or dl-form); and essential oils such as eucalyptus oil, bergamot oil, peppermint oil, cool mint oil, spearmint oil, and peppermint oil.

[0035] Examples of the disinfectant include isopropylmethylphenol, salicylic acid, benzalkonium chloride, ethanol, benzethonium chloride, gluconic acid and its derivatives, photosensitizer No. 101, photosensitizer No. 201, paraben, phenoxyethanol, piroctoolamine, and miconazole.

[0036] The vitamins may be either water-soluble or oil-soluble, and examples thereof include vitamin B6, pantothenic acid, nicotinic acid, vitamin B1, vitamin B2, biotin, folic acid, vitamin B12, water-soluble vitamin C, oil-soluble vitamin C, vitamin K; and vitamin-like factors such as ferulic acid.

[0037] Examples of the organic acids include gluconic acid, aspartic acid, aminoethylsulfonic acid, citric acid, glutamic acid, succinic acid, oxalic acid, fumaric acid, propionic acid, malic acid, salicylic acid, glycolic acid, phytic acid, tartaric acid, acetic acid, lactic acid, and salts thereof. Examples of the salts include salts of mineral acids such as sulfuric acid, hydrochloric acid, and phosphoric acid, salts of organic acids such as maleic acid and methanesulfonic acid, salts of alkali metals such as sodium and potassium, alkaline earth metal salts, and ammonium salts.

[0038] Examples of the moisturizing components include polymeric compounds such as sodium hyaluronate, heparinoids, sodium chondroitin sulfate, collagen, elastin, keratin, chitin, chitosan, and proteoglycans; amino acids such as glycine, aspartic acid, and arginine; natural moisturizing factors such as sodium lactate, urea, and sodium pyrrolidonecarboxylate; lipids such as ceramide, cholesterol, and phospholipids; plant extracts such as chamomile extract, witch hazel extract, tea extract, and perilla extract; and polyhydric alcohols such as glycerin.

[0039] The polyhydric alcohol preferably has 2 to 10 carbon atoms, and examples thereof include glycerin, diglycerin, triglycerin, propylene glycol, dipropylene glycol, 1,3-butanediol, ethylene glycol, diethylene glycol, isoprene glycol, 1,3-butylene glycol, sorbitol, xylitol, erythritol, mannitol, pentanediol, hexanediol, octanediol, decanediol, and neopentyl glycol.

[0040] Examples of the scrubbing agent include apricot kernel powder, almond shell powder, apricot kernel powder, sodium chloride granules, olive kernel powder, seawater dried granules, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, charcoal powder, hazelnut shell powder, polyethylene powder, and silicic anhydride.

[0041] Examples of the ultraviolet absorbing component include ethylhexyl methoxycinnamate, diethylaminohydroxybenzoylhexyl benzoate, bisethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, t-butylmethoxydibenzoylmethane, octocrylene, polysilicone-15, methylenebisbenzotriazolyltetramethylbutylphenol, dimethoxybenzylidene dioxoimidazolidine octyl propionate, and phenylbenzimidazole sulfonic acid.

[0042] Examples of the ultraviolet scattering component include inorganic compounds such as hydrous silicic acid, zinc silicate, cerium silicate, titanium silicate, zirconium oxide, cerium oxide, titanium oxide, iron oxide, zinc oxide, and silicic anhydride; these inorganic compounds coated with inorganic powder such as hydrous silicic acid, aluminum hydroxide, mica, and talc; compounds composited with resin powder such as polyamide, polyethylene, polyester, polystyrene, and nylon; and compounds further treated with silicone oil, aluminum salt of a fatty acid, or the like.

[0043] Examples of the astringent ingredients include alum, zinc sulfate, aluminum chloride, zinc sulfocarbonate, and tannic acid.

[0044] Examples of the above peptides or derivatives thereof include keratin hydrolyzed peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin hydrolyzed peptides, collagen hydrolyzed peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin hydrolyzed peptides, conchiolin hydrolyzed peptides, hydrolyzed conchiolin, silk proteolytic peptides, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soybean proteolytic peptides, hydrolyzed soybean protein, wheat protein, wheat proteolytic peptides, hydrolyzed wheat protein, casein hydrolyzed peptides, and acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.).

[0045] Examples of the amino acids or derivatives thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, and creatine.

[0046] Examples of the cleansing component include soaps selected from alkali metal salts such as potassium laurate, potassium myristate, potassium palmitate, and potassium stearate, alkanolamide salts, and amino acid salts; amino acid surfactants such as sodium cocoyl glutamate and sodium cocoyl methyl taurate; ether sulfate ester salts such as sodium laureth sulfate; ether carboxylate salts such as sodium lauryl ether acetate; sulfosuccinate ester salts such as sodium alkyl sulfosuccinate; fatty acid alkanolamides such as coconut oil fatty acid monoethanolamide and coconut oil fatty acid diethanolamide; monoalkyl phosphate ester salts such as sodium lauryl phosphate and sodium polyoxyethylene lauryl ether phosphate; betaine-type amphoteric surfactants such as coconut oil fatty acid amidopropyl dimethylaminoacetic acid betaine, lauryl dimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, lauryl hydroxysulfobetaine, and sodium lauroylamidoethyl hydroxyethyl carboxymethyl betaine hydroxypropyl phosphate; and amino acid amphoteric surfactants such as sodium lauryl aminopropionate.

[0047] Examples of the keratin softening ingredients include lactic acid, salicylic acid, glycolic acid, citric acid, malic acid, fruit acids, phytic acid, urea, sulfur, and the like.

[0048] Examples of the cell activation components include amino acids such as γ-aminobutyric acid; vitamins such as retinol, thiamine, riboflavin, pyridoxine hydrochloride, and pantothenic acids; α-hydroxy acids such as glycolic acid and lactic acid; tannins, flavonoids, saponins, and photosensitizer No. 301.

[0049] Examples of the blood circulation-promoting component include components derived from plants (e.g., ginseng, angelica tree, arnica, ginkgo, fennel, emmeisou, Dutch oak, chamomile, Roman chamomile, carrot, gentian, burdock, rice, hawthorn, shiitake mushroom, ginger, European hawthorn, European juniper, cnidium rhizome, Swertia japonica, thyme, clove, tangerine peel, chili pepper, angelica acutiloba, peach kernel, spruce, carrot, garlic, butcher's broom, grape, peony, horse chestnut, melissa, yuzu, coix seed, ryokucha, rosemary, rose hip, tangerine peel, angelica acutiloba, spruce, peach, apricot, walnut, corn, etc.); acetylcholine, ichthammol, cantharides tincture, gamma-oryzanol, cepharanthine, tolazoline, tocopherol nicotinate, glucosyl hesperidin, etc.).

[0050] [Method of manufacturing the cosmetic composition] The method for producing the cosmetic composition of the present invention is not particularly limited, and the composition can be produced by a conventional method by appropriately selecting and mixing the essential component, the IPL radiation-like agent, and other components to be mixed as needed (the optional components described above, the base or carrier, additives, etc. described below).

[0051] [Uses of cosmetic compositions] The cosmetic composition of the present invention is an approach inspired by IPL (light treatment) treatments used by cosmetic physicians, and is expected to have the same effects on the entire face as IPL irradiation. Use of the cosmetic composition of the present invention can achieve the effects of whitening the entire face and improving skin tone. It is also effective in improving aging symptoms (skin tone improvement) such as dullness, lack of firmness, visible pores, and dryness of the entire face. Thus, the cosmetic composition of the present invention can be used as an alternative to IPL irradiation or in combination with IPL irradiation. The cosmetic composition of the present invention used for such purposes can be suitably used as a topical composition such as a serum, lotion, emulsion, cream, gel cream, beauty mask (face mask), makeup base, foundation, sunscreen, cleanser, or detergent, and more preferably as a skin care product such as a serum, lotion, emulsion, cream, or gel cream.

[0052] The cosmetic composition of the present invention can also be made into a food composition. This food composition can be suitably used as a health food or a nutritional supplement (including a balanced nutritional diet, supplements, etc.). It is also suitable as a food with health claims (a food for specified health uses (including disease risk reduction labeling and standardized types)).

[0053] [Preparation (external composition)] When the cosmetic composition of the present invention is a composition for external use, the essential components and other optional components described above can be mixed in a conventional manner with a base or carrier commonly used in cosmetics, pharmaceuticals, and quasi-drugs, and, if necessary, with additives described below, and emulsified or solubilized as necessary to prepare a cosmetic composition in various formulations.

[0054] Examples of the base or carrier include hydrocarbons such as liquid paraffin, squalane, petrolatum, gelling hydrocarbons (such as Plastibase), ozokerite, α-olefin oligomers, and light liquid paraffin; silicone oils such as methylpolysiloxane, cyclic silicone, modified silicone, and silicone resin; oils and fats such as coconut oil, olive oil, rice bran oil, and shea butter; waxes such as jojoba oil, beaucoupon wax, candelilla wax, and lanolin; cetyl alcohol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol, octyldodecanol, isostearyl alcohol, phytosterol, and cholesterol. Examples of the esters include higher alcohols such as methyl cellulose, ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose polyvinylpyrrolidone, carrageenan, polyethylene glycol, isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, isononyl isononanoate, and pentaerythritol tetra-2-ethylhexanoate; polysaccharides such as dextrin and maltodextrin; lower alcohols such as ethanol; glycol ethers such as diethylene glycol monoethyl ether; and water.

[0055] The bases or carriers described above may be used alone or in combination of two or more. The amounts used may be appropriately selected from ranges known to those skilled in the art.

[0056] [Additives (topical compositions)] The cosmetic composition of the present invention may contain known additives added to cosmetics, pharmaceuticals, and quasi-drugs, such as surfactants, antioxidants, colorants, pearlescent agents, chelating agents, pH adjusters, preservatives, thickeners, etc., within the scope of not impairing the effects of the present invention. These additives may be used alone or in combination of two or more.

[0057] The surfactant may be any of a nonionic surfactant, a cationic surfactant, an anionic surfactant, an amphoteric surfactant, and the like, and examples thereof include sorbitan fatty acid esters such as sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, diglycerol sorbitan penta-2-ethylhexylate, and diglycerol sorbitan tetra-2-ethylhexylate; propylene glycol fatty acid esters such as propylene glycol monostearate; hydrogenated castor oil derivatives such as polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil 60 (HCO-60), and polyoxyethylene hydrogenated castor oil 80; polyoxyethylene monolaurate; Examples of surfactants include polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene (20) sorbitan (polysorbate 20), polyoxyethylene (20) sorbitan monostearate (polysorbate 60), polyoxyethylene (20) sorbitan monooleate (polysorbate 80), and polyoxyethylene (20) sorbitan isostearate; polyoxyethylene monoglyceryl cocoate; glycerin alkyl ethers; alkyl glucosides; polyoxyalkylene alkyl ethers such as polyoxyethylene cetyl ether; amines such as stearylamine and oleylamine; and silicone surfactants such as polyoxyethylene-methylpolysiloxane copolymer, lauryl PEG-9 polydimethylsiloxyethyl dimethicone, and PEG-9 polydimethylsiloxyethyl dimethicone.

[0058] Examples of the antioxidant include dibutylhydroxytoluene, butylhydroxyanisole, sorbic acid, sodium sulfite, ascorbic acid, erythorbic acid, and L-cysteine ​​hydrochloride.

[0059] Examples of the colorant include inorganic pigments and natural dyes.

[0060] Examples of the chelating agent include EDTA disodium salt, hydroxyethanediphosphonic acid calcium disodium salt, and the like.

[0061] Examples of the pH adjuster include inorganic acids (hydrochloric acid, sulfuric acid, etc.), organic acids (lactic acid, sodium lactate, citric acid, sodium citrate, succinic acid, sodium succinate, etc.), inorganic bases (potassium hydroxide, sodium hydroxide, etc.), and organic bases (triethanolamine, diisopropanolamine, triisopropanolamine, etc.).

[0062] Examples of the preservatives include benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, methyl parahydroxybenzoate, phenoxyethanol, caprylhydroxamic acid, alkanediols such as pentanediol, hexanediol, and octanediol.

[0063] Examples of the thickener include vinyl thickeners such as polyvinyl alcohol, polyvinylpyrrolidone, and carboxyvinyl polymers; cellulose thickeners such as methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose, and carboxyethyl cellulose; guar gum, pectin, pullulan, gelatin, locust bean gum, carrageenan, agar, xanthan gum, acrylates / alkyl methacrylate copolymers, polyethylene glycol, bentonite, alginic acid, propylene glycol alginate, macrogol, sodium chondroitin sulfate, hyaluronic acid, sodium hyaluronate, (hydroxyethyl acrylate / sodium acryloyldimethyltaurate) copolymer, and (ammonium acryloyldimethyltaurate / vinylpyrrolidone) copolymer.

[0064] [Formulation (external composition)] When the cosmetic composition of the present invention is a topical composition, the formulation may be any suitable one, including, for example, liquids (skin lotions, lotions, serums, etc.), creams (milky lotions, creams, gel creams, balms, etc.), gels, jellies, sherbets, aerosols, foams, sprays, patches, sticks, and other ointments and solids. These formulations can be produced according to conventional methods, such as those described in the General Provisions for Preparations in the 17th Edition of the Japanese Pharmacopoeia. Taking into account the desired stability, usability, etc., formulations such as W / O, O / W, W / O / W, and O / W / O emulsions can be appropriately selected. Patches may be prepared by impregnating a nonwoven fabric sheet, gel sheet, or the like with a serum composition, a skin lotion composition, an emulsion composition, or the like.

[0065] [Preparation (food composition)] When the cosmetic composition of the present invention is a food composition, its essential components and the other optional components described above can be formulated by mixing them with a base or carrier commonly used in foods, and, if necessary, food additives such as fats and oils, emulsifiers, dispersants, suspending agents, stabilizers, thickeners, sweeteners, colorants, flavorings, preservatives, antioxidants, and organic acids. The food additives are not limited to those listed above and the specific components described below, and can be selected appropriately depending on the purpose and needs. These food additives can be used alone or in combination of two or more. Furthermore, other active ingredients can be added to the formulation.

[0066] Examples of the fats and oils include natural vegetable oils such as peanut oil, cocoa butter, rice germ oil, perilla oil, and linseed oil, as well as hardened oils thereof, glycerides (such as glycerides, diglycerides, and triglycerides) of fatty acids (including medium-chain fatty acids), and beeswax. Of these, beeswax is preferred.

[0067] Examples of the emulsifier, dispersant, suspending agent, and stabilizer include polyhydric alcohols such as polyethylene glycol, propylene glycol, glycerin, and sorbitol; glycerin fatty acid esters; and sodium carboxymethylcellulose. Of these, glycerin, glycerin fatty acid esters, and sodium carboxymethylcellulose are preferred.

[0068] Examples of the thickener include locust bean gum, guar gum, xanthan gum, tamarind gum, pectin, carrageenan, etc. Among these, xanthan gum is preferred.

[0069] Examples of the sweetener include sucrose, fructose, maltose, erythritol, trehalose, maltitol, licorice extract, stevia processed sweetener, aspartame, acesulfame potassium, sorbitol, and xylitol.

[0070] Examples of the preservative include benzoic acid or a salt thereof, sorbic acid or a salt thereof, butyl parahydroxybenzoate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, ethyl parahydroxybenzoate, and methyl parahydroxybenzoate.

[0071] Examples of the antioxidant include ascorbic acid, sodium ascorbate, ascorbic acid stearate, sodium ascorbic acid stearate, ascorbic acid palmitate, hydrogen sulfite, sodium hyposulfite, sulfur dioxide, calcium disodium EDTA, erythorbic acid, sodium erythorbate, tocopherol, etc. Among these, ascorbic acid, ascorbic acid palmitate, and tocopherol are preferred.

[0072] Examples of organic acids include citric acid, succinic acid, tartaric acid, aspartic acid, lactic acid, malic acid, malonic acid, fumaric acid, and maleic acid.

[0073] [pH] The pH of the cosmetic composition of the present invention is usually 3.0 to 8.0, and preferably 3.5 to 7.5. This pH can be adjusted, for example, by using a pH adjuster as described below. However, this does not apply to formulations in which pH measurement is impossible or difficult.

[0074] <Screening method> The present invention also includes a method for screening for skin tone-enhancing ingredients and a method for screening for skin tone-improving ingredients. The screening method of the present invention is characterized by using, as an indicator, a gene whose expression changes when skin cells are irradiated with IPL, and includes at least a step (1) of applying a test substance to cultured skin cells and a step (2) of measuring the expression of the gene in the cultured skin cells. The screening method of the present invention makes it possible to efficiently obtain skin tone-enhancing ingredients and skin tone-improving ingredients that provide effects similar to those obtained by IPL irradiation (phototherapy).

[0075] Step (1): The cultured skin cells are not particularly limited as long as they are a cell population containing melanocytes, but examples include epidermal cells, dermal cells, and cultured skin models. It is more preferable to use MEL-300A (MatTek) or EFT-400 (MatTek) as the cultured skin model. A test substance is added to the cultured skin cells, and the cells are cultured for approximately 2 to 72 hours. A negative control is prepared without the addition of the test substance.

[0076] Step (2): After step (1), cells are collected, RNA is extracted according to standard methods, and gene expression is analyzed using a DNA microarray method or the like. The gene expression of the group to which the test substance was added in step (1) is compared with that of the group to which the test substance was not added (negative control). The genes to be compared are those whose changes have been confirmed to occur when skin cells are irradiated with IPL. Examples of such genes include OCA2, SLC45A2, and RAB27. A, such as HB-EGF. These genes are genes that the inventors have found to be suppressed or promoted in expression by IPL irradiation. In the screening method of the present invention, in the group to which the test substance is added, it is confirmed whether the expression of each gene fluctuates in the same manner as during IPL irradiation. And a test substance that fluctuates the expression of these genes in the same manner as during IPL irradiation can be determined as a component for improving skin tone and a component for improving skin tone.

[0077] <Beauty method> The present invention also includes a beauty method for improving and enhancing the skin condition, which is characterized by using an IPL irradiation-like agent. According to the beauty method of the present invention, by using an IPL irradiation-like agent, the same effects as IPL irradiation can be obtained. For example, whitening of the whole face and an effect of improving skin tone can be obtained. In addition, an improvement effect (skin tone improvement effect) on aging symptoms such as dullness of the whole face, uneven texture, fine wrinkles, and telangiectasia can be obtained. The specific description of the above IPL irradiation-like agent can be directly applied to the description in the section of the above beauty composition.

[0078] <IPL irradiation-like agent> The present invention also includes an IPL irradiation-like agent containing various plant extracts as active ingredients. As the above plant extracts, plant extracts containing flavonoids and seaweed extracts are preferable. Further, as the plant extract containing the above flavonoids, at least one plant extract selected from the group consisting of amacha extract, artichoke extract, licorice root extract, rose hip extract, white willow bark extract, neem leaf extract, mandarin orange peel extract, saxifrage extract, common mulberry seed extract, kudamono torikofira leaf extract, rosemary extract, hollyhock extract and burdock extract is more preferable. Examples of the above seaweed extracts include brown algae (gagome kombu, lishiri kombu, mitsui shikombu, macro kelp, naga kombu, wakame, etc.), red algae ( tengusa, etc.), green algae (aonori, etc.), blue-green algae (spirulina, etc.), etc. Among them, brown algae are more preferable.

[0079] That is, the IPL irradiation-mimetic agent of the present invention preferably contains, as an active ingredient, at least one plant extract selected from the group consisting of Gynostemma pentaphyllum extract, artichoke extract, licorice root extract, Rosa multiflora fruit extract, White willow bark extract, Melia azadirachta leaf extract, Mandarin orange peel extract, Saxifraga extract, Plantago major seed extract, Passiflora edulis fruit extract, Hop flower extract, Tambourissa trichophylla leaf extract, Rosemary extract, Burdock extract, and Althaea officinalis extract.Furthermore, the IPL irradiation-mimetic agent of the present invention preferably contains, as an active ingredient, at least one seaweed extract selected from the group consisting of brown algae, red algae, green algae, and blue-green algae, and more preferably contains, as an active ingredient, a seaweed extract from brown algae.

[0080] For details about the plant extract and seaweed extract contained as active ingredients in the IPL radiation-mimetic agent of the present invention, the explanation about the IPL radiation-mimetic agent in the cosmetic composition section above can be applied.

[0081] The IPL irradiation-mimetic agent of the present invention can provide the same effects as IPL irradiation, and therefore, when a cosmetic composition containing it is used, it can provide, for example, whitening of the entire face, improving skin tone, and improving aging symptoms such as dullness of the entire face, lack of firmness, noticeable pores, and dryness (skin tone improvement effect). Note that the specific description of the IPL irradiation-mimetic agent can be applied as is to the description in the above section on cosmetic compositions. [Example]

[0082] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0083] [Test 1] Analysis of changes in gene expression in epidermal cells due to IPL irradiation The following test was performed using an epidermal model containing human melanocytes (MatTek; MEL-300A). Specifically, after arrival, the MEL-300A was acclimated to 37°C in an incubator set to 5% CO2, and cultured in liquid medium (Kurabo; EPI-100LLMM). After acclimation, the epidermal sheet in the transwell was excised and placed on gauze soaked in liquid medium, and irradiated under conditions consistent with actual treatment. The epidermal model prepared using the above method was irradiated with light (IPL 560 nm) using a λ560 nm filter for 3 ms, followed by a 20 ms delay and another 3 ms. Cells were harvested 6 and 24 hours later. IPL irradiation was performed using a Photofacial M22 (Luminous). RNA was extracted from each cell line according to standard procedures, and gene expression was analyzed using DNA microarrays. A Human Gene 2.0ST Array was used for the DNA microarray.

[0084] The genes whose expression levels were altered in epidermal cells by 560 nm IPL irradiation were selected as OCA2, RAB27A, SLC45A2, and HB-EGF.

[0085] [Test 2] Screening for substances that affect the expression of OCA2, SLC45A2, and RAB27A genes in human melanocytes Normal human adult epidermal melanocytes (Kurabo Industries, Ltd.: NHEM) were seeded at 40,000 cells / well in a 24-well plate (Corning) using melanocyte medium (Kurabo Industries, Ltd.: DermaLife Ma) and cultured for 2 days. Each extract sample was then prepared in the medium to the desired concentration, filtered through a 0.2 μm pore filter, and added to the 24-well plate at 400 μL / well. After further culture for 24 hours, the cells were harvested. RNA was extracted using standard methods, cDNA was synthesized from the RNA by reverse transcription, and the expression levels of OCA2, SLC45A2, and RAB27A genes were quantified by quantitative real-time PCR. The obtained values ​​were used to calculate the ratio to the blank. Note that the blank refers to the data from a control test using only medium without the addition of each extract sample. The results are shown in Tables 1 to 17 below.

[0086] [Table 1]

[0087] [Table 2]

[0088] [Table 3]

[0089] [Table 4]

[0090] [Table 5]

[0091] [Table 6]

[0092] Table 7

[0093] Table 8

[0094] Table 9

[0095] Table 10

[0096] Table 11

[0097] Table 12

[0098] Table 13

[0099] Table 14

[0100] Table 15

[0101] Table 16

[0102] Table 17

[0103] [Test 3] Screening for substances that affect HB-EGF gene expression in human keratinocytes Normal human adult epidermal cells (NHEK, Kurabo Industries, Ltd.) were seeded at 40,000 cells / well in a 24-well plate (Corning, Kurabo Industries, Ltd.) using keratinocyte culture medium (Humedia KG2, Kurabo Industries, Ltd.) and cultured for 1 day. Each extract sample was then prepared in the culture medium to the desired concentration, filtered through a 0.2 μm pore size filter, and added to the 24-well plate at 400 μL / well. After further culture for 24 hours, the cells were harvested. RNA was extracted using standard methods, cDNA was synthesized from the RNA by reverse transcription, and the expression levels of each HB-EGF gene were quantified by quantitative real-time PCR. The obtained values ​​were used to calculate the percentage relative to the blank. Note that the blank refers to the data from a control test using only medium without any extract sample. The results are shown in Table 18 below.

[0104] [Table 18]

[0105] From the above, the cosmetic composition and cosmetic method of the present invention can be expected to have the effect of brightening skin that is naturally darker in tone (skin tone improving effect), and the cosmetic composition of the present invention can also be used as a skin tone improver.Furthermore, the cosmetic composition and cosmetic method of the present invention can be expected to have the effect of improving aging symptoms such as dullness of the entire face, loss of firmness, noticeable pores, and dryness (skin tone improving effect), and can also be used as a skin tone improver.

[0106] Examples of formulations for the cosmetic composition of the present invention are shown below.

[0107] Prescription example 1 (Beauty serum) L-ascorbic acid 10.0 (w / w%) Hydroquinone 0.1 Diethylene glycol monoethyl ether 51.0 Propylene glycol 23.0 Jiaogulan extract 0.1 Rosa multiflora fruit extract 0.5 Remaining purified water Total 100.0%

[0108] Prescription example 2 (Beauty serum) 3-O-Ethyl ascorbic acid 3.0 (w / w%) Diethylene glycol monoethyl ether 30.0 Propylene glycol 20.0 Glycerin 4.0 Althea Extract 1.0 Artichoke Extract 10.0 Remaining purified water Total 100.0%

[0109] Prescription example 3 (lotion) Tranexamic acid 1.5 (w / w%) Salicylic acid 0.1 Sodium hyaluronate 0.2 Glycerin 2.0 1,3-butylene glycol 10.0 Polyoxypropylene methyl glucoside 0.2 Acetylated sodium hyaluronate 0.01 Hydroxyethyl cellulose 0.05 Methyl parahydroxybenzoate 0.15 Flavoring agent 0.03 Oil-soluble licorice extract 0.0001 Remaining purified water Total 100.0%

[0110] Prescription example 4 (lotion) Tranexamic acid 1.5 (w / w%) Salicylic acid 0.1 Sodium hyaluronate 0.2 Glycerin 1.0 Diglycerin 0.5 1,3-butylene glycol 5.0 Dipropylene Glycol 3.0 Trehalose 0.1 Acetylated sodium hyaluronate 0.01 Hydroxyethyl cellulose 0.05 Methyl parahydroxybenzoate 0.15 Flavoring agent 0.03 Seaweed extract 0.5 Rosemary extract 0.3 Remaining purified water Total 100.0%

[0111] Prescription example 5 (lotion) Magnesium ascorbyl phosphate 3.0 (w / w%) Placenta extract 0.5 Trimethylglycine 3.0 Hyaluronic acid 0.05 Gagome kelp extract 0.3 1,3-butylene glycol 5.0 Polyoxyethylene hydrogenated castor oil 1.0 Methyl parahydroxybenzoate 0.05 White willow bark extract 20.0 Licorice Root Extract 0.01 Remaining purified water Total 100.0%

[0112] Formulation example 6 (lotion) Tranexamic acid 2.0 (w / w%) β-Arbutin 0.1 Salicylic acid 0.2 Sodium hyaluronate 0.2 Dipropylene Glycol 5.0 1,3-butylene glycol 8.0 Glycerin 3.0 Glyceryl Monostearate 1.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.05 Meadowfoam Oil 5.0 Glyceryl tri-2-ethylhexanoate 5.0 Methylpolysiloxane 1.0 Triethanolamine 0.1 Tocopherol 0.1 Acetylated sodium hyaluronate 0.05 Sodium edetate 0.05 Methyl parahydroxybenzoate 0.2 Flavoring agent 0.05 Rosemary extract 0.1 Ageratum extract 0.1 Remaining purified water Total 100.0%

[0113] Prescription example 7 (cream) Nicotinamide 1.0 (w / w%) Glycerin 5.0 1,3-butylene glycol 5.0 Light Liquid Paraffin 3.0 Acrylic acid / alkyl methacrylate copolymer 0.3 Arginine 0.3 Polyoxyethylene hydrogenated castor oil 0.5 Methyl parahydroxybenzoate 0.2 Melia Azadirachta Leaf Extract 0.5 Mandarin Orange Peel Extract 3.0 Purified water (appropriate amount) Total 100.0%

[0114] Prescription example 8 (cream) Tranexamic acid 1.0 (w / w%) Arbutin 3.0 Salicylic acid 0.05 Sodium hyaluronate 0.15 Dipropylene glycol 8.0 Diglycerin 5.0 Glycerin 3.0 Hydroxyethylurea 3.0 Polyoxyethylene sorbitan isostearate 0.5 Glyceryl monostearate 0.4 Cetostearyl Glucoside / Cetostearyl Alcohol 1.5 Acrylic acid / alkyl methacrylate copolymer 0.5 Meadowfoam oil 0.5 Squalane 3.0 Caprylic / Capric Triglyceride 1.5 Methylpolysiloxane 1.0 Behenyl alcohol 0.2 Stearyl alcohol 0.3 Xanthan gum 0.2 Sodium hydroxide 0.1 Sodium edetate 0.1 Methyl parahydroxybenzoate 0.2 Flavoring agent 0.1 Ceramide 1 0.01 Ceramide 2 0.01 Ceramide 6 0.01 Rosemary extract 0.001 Saxifraga extract 0.01 Tambourissa Trichophylla Leaf Extract 0.001 Ageratum Extract 5.0 Remaining purified water Total 100.0%

[0115] Prescription example 9 (serum) Nicotinamide 5.0 (w / w%) Glycerin 2.0 1,3-butylene glycol 3.0 Squalane 2.0 Triethylhexanoin 1.0 Carboxyvinyl polymer 0.2 (Ammonium acryloyldimethyltaurate / vinylpyrrolidone) Copolymer 0.2 Polyoxyethylene polyoxypropylene butyl ether (17E.O.) (17P.O.) 0.5 Polyoxypropylene methyl glucoside 0.5 Triethanolamine 0.15 Hydrogenated soybean phospholipid 0.5 Methyl parahydroxybenzoate 0.2 Bilberry Leaf Extract 0.05 Comfrey Leaf Extract 0.05 Purified water (appropriate amount) Total 100.0%

[0116] Prescription example 10 (serum) Nicotinamide 5.0 (w / w%) Dipropylene Glycol 5.0 1,3-butylene glycol 5.0 Light Liquid Paraffin 2.0 Methylphenylpolysiloxane 1.0 Xanthan gum 0.5 (PEG-240 / Decyltetradeceth-20 / HDI) copolymer 1.5 Cholesteryl oleate 0.2 Polyoxyethylene hydrogenated castor oil 0.5 Hydrogenated soybean phospholipids 0.2 Pentanediol 1.0 Phenoxyethanol 0.2 Macadamia nut fatty acid phytosteryl 0.1 Geranium globulus extract 0.05 Licorice Root Extract 0.05 Purified water (appropriate amount) Total 100.0%

[0117] Prescription example 11 (cream) Nicotinamide 5.0 (w / w%) Glycerin 5.0 1,3-butylene glycol 10.0 1,2-Pentanediol 0.5 Plant-based squalane 5.0 Caprylic / Capric Triglyceride 1.0 Methylpolysiloxane 1.0 Carboxyvinyl polymer 0.4 Xanthan gum 0.05 Polyoxyethylene sorbitan monostearate 1.5 Sorbitan monostearate 0.5 Polyoxyethylene cetyl ether 0.2 Glycerin Mono 2-Ethylhexyl Ether 0.5 Ceramide 2 0.0005 Ceramide 6II 0.00005 Seaweed extract (1) 2.0% Althea extract 0.01 pH adjuster (appropriate amount) Purified water (appropriate amount) Total 100.0%

[0118] Formulation example 12 (sunscreen) Ascorbyl tetra-2-hexyldecanoate 0.1 (w / w%) Trimethylglycine 1.0 2-Ethylhexyl paramethoxycinnamate 10.0 Decamethylcyclopentasiloxane 20.0 Octyl palmitate 10.0 Polyoxyethylene-methylpolysiloxane copolymer 3.0 Methylhydrogenpolysiloxane treatment Low-temperature fired zinc oxide 15.0 Polyalkyl acrylate 5.0 Glycerin 5.0 Absolute ethanol 5.0 1,3-butylene glycol 3.0 Panthenol 0.1 Fragrance 0.1 Ceramide 2 0.001 Ceramide 3 0.001 Ceramide 6 0.001 Plantago major seed extract 0.01 Passiflora edulis fruit extract 0.1 Remaining purified water Total 100.0%

[0119] Prescription example 13 (cleansing) L-Ascorbic acid 2-glucoside 0.1(w / w%) PEG-20 Glyceryl Triisostearate 12.0 Polyglyceryl-10 Isostearate 8.0 Ethylhexyl palmitate 30.0 Triethylhexanoin 20.0 Mineral oil 25.0 Diphenylsiloxyphenyl Trimethicone 1.0 Hop flower extract 0.0001 Remaining purified water Total 100.0%

[0120] Prescription example 14 (cleansing) L-Ascorbic acid 2-glucoside 0.1(w / w%) PEG-20 Glyceryl Triisostearate 12.0 Polyglyceryl-10 Isostearate 8.0 Ethylhexyl palmitate 28.0 Triethylhexanoin 20.0 Mineral oil 20.0 Diphenylsiloxyphenyl Trimethicone 1.0 Seaweed extract 0.0001 Althea extract 0.0001 Remaining purified water Total 100.0%

[0121] Formulation Example 15 (Soft capsule: food composition) The numerical values ​​in the following recipe examples of food compositions are in parts by weight. Capsule contents Evening primrose oil 120 Hydrolyzable Tannins 20 Hydrolyzed Soy Protein 20 Lotus Germ Extract 5 Ceramide 3 Hyaluronic acid 0.5 CoQ10 2 Haematococcus algae pigment extract (containing astaxanthin) 4 Vitamin C 1 Glycerin fatty acid ester 10 Beeswax 5 Capsule membrane Gelatin 92 Pullulan 3 Glycerin 35 coloring agent 3

[0122] Prescription example 16 (beauty drink) unit (mg) Licorice Extract 180 Placenta extract 180 Collagen Peptide 1000 Artichoke Extract 50 Proteoglycan 20 Jiaogulan Extract 20 Polysorbate 80 0.1 Erythritol 3 Agar 0.5 Starch syrup 0.6 fructose 2 Acidulant 0.5 Fragrance 0.1 Water 86.1 Total daily intake (mg) 1542.9

[0123] Prescription example 17 (beauty drink) unit (mg) Seaweed Extract 180 Placenta extract 180 Collagen Peptide 1000 Proteoglycan 20 Rosemary Extract 40 Polysorbate 80 0.1 Erythritol 3 Agar 0.5 Starch syrup 0.6 fructose 2 Acidulant 0.5 Fragrance 0.1 Water 103.2 Total daily intake (mg) 1530.0

Claims

[Claim 1] At least one plant extract selected from the group consisting of Jiaogulan extract, artichoke extract, licorice root extract, Multiflora rose fruit extract, White willow bark extract, Melia azadirachta leaf extract, Mandarin orange peel extract, Saxifraga extract, Plantago major seed extract, Passiflora edulis fruit extract, Hop flower extract, Tambourissa trichophylla leaf extract, Rosemary extract, Burdock extract, and Althaea extract, and / or brown algae, green algae, and blue-green algae A composition for regulating the expression of at least one melanin-related molecule in skin cells selected from the group consisting of OCA2, SLC45A2, RAB27A, and HB-EGF, the expression of which is suppressed or promoted by IPL irradiation, comprising as an active ingredient at least one seaweed extract selected from the group consisting of: OCA2, SLC45A2, RAB27A, and HB-EGF, wherein, in regulating the expression of the melanin-related molecules, the expression of OCA2, SLC45A2, and RAB27A is suppressed, and the expression of HB-EGF is promoted.

Images