MICROALGAE-BASED SOIL INOCULATION SYSTEM AND METHODS OF USE

Abstract

Some embodiments include a microalgae cultivation system comprising a bioreactor adapted to propagate microalgae in a culture solution using a combination of at least one type of natural and artificial light, and at least one nutrient source, including at least one carbon source, where the microalgae are freely suspended in and form part of the culture solution. A microalgae feeder is coupled to the bioreactor, and a first controller is positioned between a water conditioning assembly and the bioreactor. The water conditioning assembly is coupled as a water supply inlet to the bioreactor and is configured to condition the water supply to a specific purity that allows for substantially unimpeded growth of the microalgae in the culture solution at a specific concentration. The first controller is configured to regulate the delivery of the microalgae feeder to the bioreactor.

Description

MICROALGAE-BASED SOIL INOCULATION SYSTEM AND METHODS OF USE RELATED APPLICATIONS This application claims the benefit and priority of U.S. Provisional Application No. 62 / 806,543, filed on February 15, 2019, entitled “Method of Isolation, Selection, and Use of Endemic Microbes for Agriculture Production Areas.” This application claims priority to U.S. Application No. 16 / 534,907, filed August 7, 2019, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” which is a continuation application in part of U.S. Patent Application No. 16 / 207,528, filed December 3, 2018, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” which is a continuation of U.S. Patent Application No. 14 / 069,932, filed November 1, 2013, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” now issued as U.S. Patent No. 10,172,304, which is a continuation of International Patent Application No.PCT / US12 / 36293, filed May 3, 2012, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” which claims the benefit and priority of U.S. Provisional Application No. 61 / 481,998, filed May 3, 2011, entitled “Microalgae-Based Soil Inoculating System and Methods of Use,” and is also a continuation in part of U.S. Patent Application No. 15 / 647,005, filed July 11, 2017, entitled “Soil Enrichment Systems and Methods,” this application incorporates the description of all these priority applications by reference. However, to the extent that the present description conflicts with any referenced application, priority shall be given to the present description. BACKGROUND OF THE INVENTION Microbes in the soil have many well-known beneficial effects. While there are many references to algae herein, these references are used only as helpful examples and do not limit the scope of the inventions described and claimed herein, which also relate to microbes in general. Algae have the ability to adapt to their environment. For example, algae found in the soil of southwestern deserts have adapted to high temperatures, alkaline pH levels, and periods of desiccation, while algae in northern climates have adapted to much lower temperatures, freeze-thaw cycles, higher soil moisture levels, and more acidic soil pH levels, etc. Native algae fill a niche in the field ecosystem. Within the soil ecosystem, they have developed a symbiosis with other organisms, resulting in a biochemical environment where compounds produced by native algae can enhance the growth of other beneficial microbes and suppress the growth of undesirable or non-beneficial organisms. For example, algae are known to produce biochemicals such as amino acids, hormones, peptides, and fatty acids that promote the growth of beneficial microorganisms. These beneficial biochemicals also directly benefit crop plants. Beneficial microorganisms produce biochemicals that both algae and crops can utilize for growth (e.g., sugars and vitamins), resulting in the continuous growth of both algae and crops.At the same time, algae can produce compounds that are antibacterial, antifungal, algicidal and / or antiprotozoal that prevent the growth of unwanted microbes in soil and surface waters. When soil algae die, they release cellular biochemicals that can directly nourish the soil biome and any crops growing in it. These biochemicals are large molecules (e.g., proteins, fats, pigments, peptides, nucleic acids, etc.), some or all of which can be absorbed by the crop, resulting in crops with higher nutritional value. If foreign live algae are introduced into the soil, the ecosystem is forced to rebalance. This imbalance can lead to the production of one or more unwanted biochemicals (such as a toxin), or the absence of an important biochemical that may be required by the crop. When algae are introduced into the soil, metabolic activity in the soil increases, resulting in greater CO2 production. This is particularly true for live algae, whose metabolic activities continue after introduction into the soil. This CO2 production lowers the soil pH, resulting in the dissolution of calcium and magnesium carbonate bonds, thus opening the soil for greater root penetration and increased movement of water and fertilizer. This increased water movement carries more salts out of the root zone, thereby reducing osmolarity within the root zone and increasing the bioavailability of macro- and micronutrients to the crop. The lower pH also releases bound potassium and phosphorus, making them available to plants. Algae excrete extracellular phosphatases almost immediately after the onset of phosphorus-limited conditions.These compounds release phosphates from soil particles and make them available to plants. Green algae also produce polysaccharides that remain in the water until needed. The substantially constant or periodic addition of algae can result in a desirable accumulation of organic matter (humus) within the soil that also has the property of retaining water and nutrients that can be released to plants as needed. Other methods for incorporating humus into the soil generally require tilling into organic matter (compost, various plant cuttings, manure, etc.), which is best done when a field is located between crops. Humus aids in the formation of natural iron chelates (fulvic acids-Fe), which prevents the soil from becoming blocked by calcium and magnesium carbonates, thus avoiding chlorosis problems caused by the low bioavailability of these nutrients. Chlorosis is the reduction in the green color of plants due to a decrease in the amount of chlorophyll in the leaves, caused by a lack of bioavailable macro- and micronutrients such as nitrogen (N), magnesium (Mg), calcium (Ca), and iron (Fe), even when these nutrients are present in the soil. Ion exchange capacity is a quantitative measure of how well fertilizer elements bind to soil particles for storage and release. The ion exchange capacity of humus (e.g., 400 to 600 meq / 100g) is 5 to 10 times greater than that of clay (e.g., 50 to 150 meq / 100g). This capacity allows fertilizers to be retained in the soil for use by plants as needed. As plants utilize nitrogen (N), phosphorus (P), and potassium (K) in the soil, the stored elements are released from the humus as required. By combining with humic substances, copper and other trace elements become less toxic and more readily available to plants. Fertilizers are more effective when combined with microalgae. Algal cells process fertilizers by breaking down certain molecules into more bioavailable forms that plants can more easily use. The nutrients are then more efficiently absorbed and fully available to the plant's root system. For example, ammonium nitrate, an excellent source of nitrogen, is one of the most common bulk fertilizers used in agriculture. While plants can readily absorb the nitrate in this fertilizer, the ammonium component is less accessible to the plant. Microalgae cells will absorb the ammonium, naturally convert it into nitrogenous biochemicals, and upon their death, release these valuable biochemicals to the plant for easy uptake.Furthermore, nutrients from fertilizers can bind to microalgae cells or their organic remains and are less likely to be lost in runoff water during rain or irrigation. After they die, the algae can also feed bacteria in the soil, which can convert ammonium ions into nitrate ions. Algae produce growth regulators (e.g., gibberellic acid) that improve salt tolerance, induce seed germination, and increase plant growth rate and fruit production. Artificial or concentrated growth regulators are expensive, especially when applied in substantial quantities, making it impractical for growers to replicate this effect using other products. Algae play a role in agricultural pest control by directly producing antibiotics and antifungal compounds, and by feeding beneficial soil microbes that produce other pest-fighting compounds. These compounds give plants the ability to prevent invasion by pathogenic species. Disease and pests also become more resistant due to the improved plant vigor. As discussed earlier, live microalgae cells can act as a catalyst to harness and utilize all the available benefits of standard fertilizers. They also provide a natural supply of essential compounds and phytochemicals, while supporting the overall effectiveness of the growing environment. These powerful attributes work synergistically to encourage plants to grow more abundantly and rapidly, consistently producing a more plentiful, higher-quality, and more nutrient-rich end product. The benefits of a microalgae cell additive are realized when the algae cells distributed to the soil are in a healthy, living form and present in high concentrations. The selection and formulation of the algae additive are critical to its overall impact.When properly implemented, a microalgae additive program is easy to manage and offers significant potential for improving agricultural production. The impact can be greatest in depleted soils, such as arid soils with significant salt and caliche accumulation and minimal organic matter. Furthermore, selecting native algae for propagation and distribution to an agricultural production area results in a higher survival rate and a greater, faster impact on soil health. BRIEF DESCRIPTION OF THE INVENTION Some embodiments include a cultivation system comprising a bioreactor adapted to propagate microalgae in a culture solution using a combination of natural and / or artificial light, and at least one nutrient comprising at least one carbon source, wherein the microalgae are freely suspended in and form part of the culture solution. Some embodiments include an algal nutrient supply coupled to the bioreactor and a first controller between a water conditioning assembly and the bioreactor. In some embodiments, the water conditioning assembly is coupled as a water supply inlet to the bioreactor and is configured to condition the water supply to a specific purity that allows substantially unhindered growth of the microalgae in the culture solution at a specific concentration.In addition, in some configurations, the first controller can be configured to control the distribution of algae nutrient supply to the bioreactor. In some configurations, a carbon dioxide source is coupled to the bioreactor, where carbon dioxide is injected into the culture solution as the carbon source. Some additional modalities include a second controller coupled to a probe and configured to regulate the release of carbon dioxide from the carbon dioxide source to the bioreactor based at least in part on one or more measurements from the probe, where carbon dioxide is injected into the culture solution as the carbon source. In some configurations, the probe is a pH probe configured to measure the pH of the culture solution. In some configurations, the water conditioning assembly includes an ozone generator coupled to an ozone contactor, where the ozone generator is configured to generate ozone and distribute the ozone to at least partially ozonize the supply water. Some models include a solids filter downstream of an ozone contactor outlet, where the solids filter is configured to remove solids from the ozonated supply water leaving the ozone contactor. Some models also include a carbon filter and / or a UV light system placed downstream of the solids filter, where the carbon filter and / or UV light system can at least partially deozonize the ozonated supply water. Some embodiments include at least one pressurized air supply coupled to the bioreactor, wherein the at least one pressurized air supply can generate gas bubbles to aerate and / or at least partially agitate the culture solution. In some embodiments, the gas bubbles include CO2, N2, and / or O2. Some embodiments further comprise at least one water reservoir or tank that supplies or is coupled to the supply water inlet. Some additional embodiments include a mobile trailer that supports at least the bioreactor, the water conditioning unit, and the carbon dioxide source. In some embodiments, the microalgae feedstock comprises a fertilizer, a macronutrient, a micronutrient, and at least two different microalgae species. In some formulations, the macronutrient is selected from the group consisting of phosphorus, nitrogen, carbon, silicon, calcium salt, magnesium salt, sodium salt, potassium salt, and sulfur; and one or more micronutrients are selected from the group consisting of manganese, copper, zinc, cobalt, molybdenum, vitamins, and trace elements. Additionally, in some formulations, the micronutrient comprises a vitamin and a mineral added to the conditioned supply water. Some modalities include a telemetry system configured for remote monitoring and / or control operation of one or more of the first controller, the second controller, the bioreactor, and at least one component or assembly of the water conditioning assembly. In some models, artificial light comprises LED lights placed inside the bioreactor and / or near a surface of the bioreactor that expose the microalgae to light. In some embodiments, the carbon dioxide source comprises a tank comprising carbon dioxide gas and / or a carbon dioxide generator and / or a carbon dioxide sequester that sequesters and temporarily stores atmospheric carbon dioxide. In some additional embodiments, the microalgal feedstock comprises a first type of algae and / or a second type of algae and / or bacteria and / or fungi. Some embodiments further comprise a flow imaging device coupled to a bioreactor outlet, wherein the flow imaging device is configured to create images of algae, predators, and contaminants in the culture solution for quality control monitoring. Some embodiments further comprise a microorganism mixer configured to mix algae, and / or bacteria, and / or fungi with any of the culture solution exiting the bioreactor. Some embodiments include a method comprising preparing one or more samples containing microbes from at least one location within a current or planned plant growth area, and preparing at least one cultured sample by culturing microbes from the sample. In addition, some embodiments include selecting at least one target microbe species from the at least one cultured sample and propagating the selected target microbe species to increase its concentration in the at least one cultured sample. Some embodiments include providing a bioreactor adapted for propagating the selected target species in a culture solution, wherein the selected target species is freely suspended in and forms part of the culture solution.Furthermore, some embodiments include coupling a power supply to the bioreactor and a first controller between a water conditioning assembly and the bioreactor, where the water conditioning assembly is coupled as a water supply inlet to the bioreactor and is configured to condition the water supply to a specific purity that allows substantially unhindered growth of at least one selected target species in the culture solution at a specific concentration. In addition, in some embodiments, the first controller is configured to control the supply of the power supply to the bioreactor. Furthermore, in some embodiments of the method, a carbon dioxide source is coupled to the bioreactor. Some embodiments include a second controller coupled to the probe and configured to regulate the release of carbon dioxide from the carbon dioxide source to the bioreactor based at least in part on one or more probe measurements, and furthermore, where carbon dioxide is injected into the culture solution, a carbon source that allows propagation of at least one selected target microbial species. Some embodiments include distributing at least a portion of the at least one target microbial species to at least one location, where at least a portion of the at least one target microbial species that is distributed comprises at least one live microbe. In some embodiments, the at least one live microbe is selected to be a well-adapted endemic species. In some forms of the method, the water conditioning assembly includes an ozone generator coupled to an ozone contactor, where the ozone generator is configured to generate ozone and distribute the ozone to at least partially ozonize the supply water. In some variations of the method, a solids filter is placed upstream of an inlet of the ozone contactor. In some embodiments of the method, a carbon filter and / or a UV light system are placed immediately downstream of the solids filter, where the carbon filter and / or UV light system are configured and arranged to at least partially deozonize the ozonated feed water. Additionally, at least one pressurized air supply is coupled to the bioreactor, where the pressurized air supply can generate gas bubbles to aerate and / or at least partially agitate the culture solution in the bioreactor. Some embodiments of the method further comprise distributing at least a portion of the at least one target microbe species to at least a portion of the at least one location. In some embodiments, at least a portion of the distributed target microbe species comprises at least one live microbe. In some embodiments, the at least one live microbe is an algal species endemic to the distribution location. In some additional embodiments, the at least one live microbe is a live species selected to restore a normal soil flora mixture to a farmland. In some other embodiments, the live algal species are selected for their specific properties desired to improve the soil at the delivery location. Some methods include sampling the algal flora from an agricultural location and selecting at least one desired algal species for propagation, where the desired algal species is present at the agricultural location as an initial concentration. Other methods include propagating the desired algal species in at least one bioreactor and distributing it to the agricultural location to increase the algal species concentration to a level higher than the initial concentration. In some embodiments of the method, at least one bioreactor is adapted to propagate at least one desired species in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient comprising at least one carbon source, wherein at least one desired species is freely suspended in and forms part of the culture solution. In some embodiments of the method, an algal nutrient supply is coupled to at least one bioreactor and a controller to regulate the flow between a water conditioning assembly and the at least one bioreactor. In some embodiments, the water conditioning assembly is coupled as a water supply inlet to the at least one bioreactor to condition the water supply to a specific purity that allows for substantially unhindered growth of the microalgae in the culture solution at a specific concentration. Furthermore, in some embodiments, the controller is configured to regulate the delivery of the algal nutrient supply to the at least one bioreactor. In some embodiments of the method, a carbon dioxide source is coupled to at least one bioreactor, where the carbon dioxide is injected into the culture solution as the carbon source. In some additional embodiments of the method, a second controller is coupled to a probe; the second controller is configured to regulate the release of carbon dioxide from the carbon dioxide source to the bioreactor based at least in part on one or more measurements from the probe. In some embodiments of the method, the water conditioning assembly includes an ozone generator coupled to an ozone contactor, where the ozone generator produces and distributes ozone to at least partially ozonize the supply water. In some additional embodiments of the method, a solids filter is placed upstream of an inlet to the ozone contactor. In some embodiments of the method, a carbon filter and / or a UV light system are placed downstream of the solids filter, where at least one of the carbon filter and the UV light system at least partially deozonizes the ozonated feed water. In some other embodiments of the method, a pressurized air supply is coupled to the bioreactor, where the pressurized air supply generates gas bubbles to at least partially aerate and / or agitate the culture solution in the at least one bioreactor. BRIEF DESCRIPTION OF THE FIGURES Figure 1 represents a first modality of the microalgae-based soil inoculation system of the invention. Figure 2 represents a front perspective view of a second modality of the microalgae-based soil inoculation system of the invention. Figure 3 represents a side elevation view of a third modality of the microalgae-based soil inoculation system of the invention. Figure 4A represents a field, five weeks after a melon crop was planted and treated according to the method and system of the invention. Figure 4B illustrates the same field as Figure 4A nine weeks after a melon crop was planted and treated according to the method and system of the invention. Figure 5A represents a melon plant in an untreated field section according to the invention. Figure 5B represents melon plants in a field section treated according to the invention. Figure 6A depicts a melon growing on the plant after nine weeks in an untreated field section according to the invention. Figure 6B depicts a melon growing on the plant after nine weeks in a field section treated according to the invention. Figure 7 represents a fourth modality of the microalgae-based soil inoculation system of the invention. Figure 8 represents a fifth modality of the microalgae-based soil inoculation system of the invention. Figure 9 illustrates a soil enrichment system according to some additional embodiments of the invention. DETAILED DESCRIPTION OF THE INVENTION Before explaining in detail any embodiment of the invention, it is understood that the invention is not limited in its application to the construction details and component arrangement set forth in the following description or illustrated in the following figures. The invention is capable of other embodiments and may be carried out in various ways. Furthermore, it is understood that the phraseology and terminology used herein are for descriptive purposes and are not to be considered limiting. The use of "includes," "comprising," or "having" and variations thereof herein is intended to encompass the elements listed below and their equivalents, as well as additional elements. Unless otherwise specified or limited, the terms "mounted," "connected," "supported," and "coupled," and variations thereof, are used broadly and encompass both direct and indirect mountings, connections, supports, and couplings.Furthermore, “connected” and “coupled” are not limited to physical or mechanical connections or couplings. The following description is presented to enable a person skilled in the art to perform and use embodiments of the invention. Various modifications to the illustrated embodiments will be readily apparent to such persons skilled in the art, and the general principles herein may be applied to other embodiments and applications without departing from the embodiments of the invention. Therefore, it is not intended that the embodiments of the invention be limited to MA / 1 / u» 1 The embodiments shown herein are not intended to be limited, but rather to be given the broadest scope consistent with the principles and characteristics described herein. The following detailed description should be read with reference to the figures, in which similar elements in different figures have similar reference numbers. The figures, which are not necessarily to scale, represent selected embodiments and are not intended to limit the scope of embodiments of the invention. Those skilled in the art will recognize that the examples provided herein have many useful alternatives that fall within the scope of embodiments of the invention. Some embodiments of the invention include a system capable of distributing a full range of micronutrients within microalgae to the soil. In some embodiments, the water containing microalgae (effluent) can be inoculated into the soil, making the micronutrients immediately bioavailable to crops grown in the soil. In some embodiments, the system can be placed within an irrigation system between the water source and the water holes, through which irrigation water can be applied to crops. In some embodiments, the system can produce biofertilizers that are immediately bioavailable to crops, resulting in negligible runoff pollution.Using this system, inorganic agricultural chemicals can be used more efficiently after they are converted into a bioavailable form by algae; therefore, the amount of chemicals needed is reduced. In some configurations, the system can be used to build organic soil components with nutrient-rich algal biomass to rehabilitate depleted (nutrient-poor) soils. In some configurations, the system can facilitate and accelerate the transformation of a chemical-based farm into an organic farm. In some configurations, the system can distribute microalgae to the soil, which dissolve soil carbonates, accumulate polysaccharide content in the topsoil, and improve soil porosity by up to 500% or more. In some configurations, the system also provides for the use of specific algal biotoxins instead of conventional chemical fungicides and other chemical poisons / toxins to control nematodes and other harmful pests. Some embodiments include a system that may comprise one or more bioreactors. In some embodiments, the system may comprise multiple bioreactors. In some embodiments, when multiple bioreactors are present, the bioreactors may be the same or different. Likewise, in some embodiments, the contents of the bioreactor may be the same or different. In some embodiments, the culture medium in a bioreactor of the system may comprise one or more types of microalgae. Some embodiments of the invention include those in which: a) all the microalgae are of the same type; b) two or more different types of microalgae are present; and / or c) one or more bioreactors contain one or more types of microalgae, and one or more additional bioreactors contain one or more additional types of microalgae. In some configurations, the microalgae in the bioreactor can be propagated so that an initial microalgal inoculant placed in the bioreactor can provide an endless supply of microalgae. In this case, the microalgal feed and water can be loaded into the bioreactor, and a sufficient amount of microalgal biomass can be removed from the bioreactor periodically to maintain conditions within the bioreactor suitable for microalgal cultivation. In some applications, the system and its method of use can improve total crop yield by 5% to 30% or more compared to untreated crops. In some applications, the system and its method of use can improve the texture, flavor, size, nutrient content, and / or yield of a crop compared to an untreated crop. In terms of agricultural use, in some applications, the system and its method of use can reduce total energy consumption, and / or reduce ecological pollution, and / or reduce greenhouse gas emissions, and / or increase the bioavailability of micronutrients and macronutrients, and / or reduce the use of chemical fertilizers, and / or reduce the overall cost of crop production, and / or reduce tillage costs, and / or reduce the need for and use of fungicides, herbicides, and / or pesticides, and / or reduce soil compaction, and / or improve soil porosity, and / or increase soil microbial content.and / or increase the organic content of the soil, and / or reduce the amount of irrigation water needed to grow a crop, and / or reduce the occurrence of over-fertilization, and / or reduce soil runoff and erosion, and / or improve plant characteristics and / or improve water / moisture retention by the soil, all compared to untreated crops and farmland. In some configurations, the system can be used to reduce or eliminate carbonate buildup in irrigation equipment by flowing water containing microalgae through the equipment. In some configurations, the system can also be used to reduce or eliminate carbonate buildup in the soil by inoculating the soil with water containing microalgae. Many different species and strains of microalgae can be used, depending on the needs of the crop. The algae can be collected and cultivated from the field where the crops will be grown or from commercial sources. Microalgae samples can be obtained from repositories at Arizona State University, the University of California, Berkeley, the University of Texas at Austin, the Woods Hole Oceanographic Research Institute, the Scripps Institution of Oceanography, or other repositories. Different species and strains of microalgae grow best under different conditions. The culture conditions within the bioreactor will vary according to the specific microalgal species present. Conditions for cultivating many different types of microalgae can be found in *The Handbook of Microalgal Culture: Biotechnology and Applied Phycology* (ed. Amos Richmond, Blackwell Publishing, Oxford, UK, 2004), *Algal Culturing Techniques: A Book for All Phycologists* (ed. Robert A. Andersen, Elsevier Academic Press, 2005), and *Microalgae: Biotechnology and Microbiology*, *Cambridge Studies in Biotechnology* (ed. E.W. Becker, Press Syndicate of the University of Cambridge, 1994), the descriptions of which are hereby incorporated in full by reference. In some modalities, native microalgal species may possess properties that make them optimal for growth under the environmental conditions of the target geographic location. In some modalities, algae from non-native locations or algal collections may be used to inoculate the soil of the target geographic location in order to maximize specific bioavailable compounds. Some modalities include a method for inoculating the soil that may comprise: obtaining a soil sample from a target geographic location and / or isolating a robust native microalgal species from the sample and / or culturing the microalgae to form a prime inoculant.In addition, the method may include inoculating a portable microalgae-based soil inoculation system with the first inoculant and / or cultivating the microalgae in the inoculation system to form a second inoculant and / or inoculating the soil of the target geographic location one or more times with the second inoculant. Further details are described below. In some embodiments, the system of the invention may employ various different types of water as a water source, including, but not limited to, wastewater, well water, lake water, stream water, pond water, rainwater, river water, and / or freshwater. Since the water is intended for crop growth, it is preferable that the water source have low salinity and be free of heavy metals. In some embodiments, after exiting the microalgae inoculation system, the inoculated water may be supplied to a crop by any conventional means or irrigation system used in agriculture, for example, by flooding, sprinkler or drip irrigation systems, or by spray or aerial application. If applied by spray or aerial application, the treatment may be followed by sufficient water to carry the algae to the soil. In some embodiments, the system and method can provide continuous, semi-continuous, repeated, or periodic treatment of the soil with inoculant containing microalgae. For example, in some embodiments, the soil can be treated with inoculant containing microalgae daily, or every two days, or every three days, or semi-annually, or every four days, or every fifth day, or every sixth day, or weekly, or bi-weekly, or every third week, or every fourth week, or monthly, or bi-monthly, or quarterly, or every three months. MA / 1 / u» 1 rus semi-annually, or annually. In some methods, the soil can be treated with water that does not contain the microalgae and then inoculated with water containing microalgae, or vice versa. Some methods include dilute, semi-concentrated, and concentrated algal cultures with a single algal species or two or more different algal species. In some methods, although optional, additional culture nutrients (macronutrients and / or micronutrients), apart from the microalgae feed, can be included in the irrigation water. For example, in some methods, nutrients such as calcium can be incorporated into the algal species for transport and uptake by the cultures. The following table includes examples of macronutrients and micronutrients. Macronutrients Micronutrients Nitrogen (N) Boron (B) Phosphorus (P) Sulfur (S) Potassium (K) Copper (Cu) Carbon (C) Chloride (Cl) Oxygen (O) Iron (Fe) Magnesium (Mg) Molybdenum (Mo) Calcium (Ca) Manganese (Mn) Nickel (Ni) Zinc (Zn) Selenium (Se) Carbon (Cr) Cobalt (Co) Biotin Thiamin Vitamin B12 Vitamin B6 Algae operate symbiotically with other organisms, both microorganisms and macroorganisms. While the primary focus of the invention is on algal cultivation, growing algae in a diverse community of multiple microorganisms can offer useful solutions. Nitrogen-fixing microbes, called diazotrophs, are divided into two main groups: independent and symbiotic. Aerobic diazotrophs, of which there are more than 50 genera, including Azotobacter, methane-oxidizing bacteria, and cyanobacteria, require oxygen to grow and fix nitrogen in soil when oxygen is present. Azotobacter, some related bacteria, and some cyanobacteria fix nitrogen in ordinary air, but most members of this group fix nitrogen only when the oxygen concentration is low. Aphanizomenon flosaquae reduces acetylene and fixes nitrogen in algal cultures.Some symbiotic bacteria belong to the genus Rhizobium, such as Bradyrhizobium and Sinorhizobium, which colonize the roots of leguminous plants and stimulate the formation of nodules within which they fix nitrogen microaerobically. Green microalgae provide nitrogen, phosphorus, potassium, calcium, and various other micronutrients. Consequently, some methods include cultivating one or more microalgae together with, or inoculating into the soil along with, one or more diazotropes. In some systems, suitable microorganisms that can be co-cultivated or inoculated into the soil along with microalgae and / or algae may include actinomycetes, bacteria, fungi, and / or mycorrhizae. For example, some systems include actinomycetes, which are thread-like bacteria that resemble fungi. Although not as numerous as bacteria, they play vital roles in the soil, where they help decompose organic matter into humus, which slowly releases nutrients. They also produce antibiotics to combat root diseases. These same antibiotics can be used to treat human illnesses. Actinomycetes create the sweet, earthy smell of biologically active soil when a field is tilled. Some methods may involve the use of bacteria that can break down complex molecules, allowing plants to absorb nutrients. Some species release nitrogen, sulfur, phosphorus, and trace elements from organic matter. Others break down soil minerals and release potassium, phosphorus, magnesium, calcium, and iron. Still other species produce and release natural plant growth hormones, which stimulate root growth. A few bacteria fix nitrogen in the roots of legumes, while others fix nitrogen independently of the plant association. Bacteria are responsible for converting ammonium nitrogen to nitrate and back again, depending on soil conditions. Several bacterial species increase nutrient solubility, improve soil structure, combat root diseases, and detoxify the soil.In some embodiments, the bacteria suitable for co-cultivation with the microalgae and for use in the system of the invention are described in United States Patent No. 7,736,508 to Limcaco (June 15, 2010), the relevant description of which is hereby incorporated by reference. Some methods may involve the use of fungi, some species of which appear as thread-like colonies, while others are single-celled yeasts. Slime molds and mushrooms are also fungi. Many fungi help plants by decomposing organic matter or releasing nutrients from soil minerals. Fungi are generally early adopters of larger pieces of organic matter and begin the decomposition process. Some fungi produce plant hormones, while others produce antibiotics, including penicillin. Several fungal species trap harmful plant-parasitic nematodes. Some methods may include the use of mycorrhizae, a group of fungi that live on or in plant roots and extend the reach of root hairs in the soil. Mycorrhizae increase the absorption of water and nutrients, especially in less fertile soils. Roots colonized by mycorrhizae are less likely to be penetrated by root-feeding nematodes because the pest cannot pierce the thick fungal network. Mycorrhizae also produce hormones and antibiotics, which enhance root growth and provide disease suppression. The fungi benefit from the plant association by taking nutrients and carbohydrates from the roots of the plants they live on. In addition to revitalizing or supplementing soil nutrients, some modalities of the system and method can also be used instead of, or to reduce the need for, conventional herbicides, pesticides, fungicides, and nematocides. For example, in some modalities, a species of algae with toxins specifically selected to manage nematodes and other soil predators can be applied after harvest. The algae with toxins are naturally occurring and typically die after killing the nematodes. While it is possible for the algae to mutate, native algae will be much more robust and will quickly eliminate any remaining toxic algae. Microalgae suitable for use as pesticides include algae from the genera Nostoc, Scytonema, and Hapalosiphon.Some modalities may include the use of the system and methods in locations such as soil-based farms, parks, hydroponic farms, aquaponics, nurseries, golf courses, sports fields, orchards, gardens, zoos, and other locations where crops or plants are grown. Some modalities may include the use of additional phytotoxins that can be obtained from microbes described in Duke et al. (“Chemicals from Nature for Weed Management”, Weed Science, (2002) vol. 50, pp. 138-151). Some non-limiting examples of phytotoxins include actinonin, brefeldin, carbocyclic coformycin, cerulenin, cochlioquinone, coronatin, 1,4-cineole, fischerelin, fumosin, fusicoccin, gabaculin, gostatin, grandinol, hydantocidin, leptospermone, phaseolotoxin, phosphinothricin, podophyllotoxin, prehelmintosporol, pinozidin, quassinoid, rhizobitoxin, tagetitoxin, sorgoleone, syngotoxin, tentoxin, tricolorin A, thiolactomycin, and usnic acid. Some methods may include the use of a bioreactor adapted to receive and utilize natural and / or artificial light. As such, in some methods, the bioreactor may be adapted to allow the exposure of microalgae to a light source. In some methods, the bioreactor wall may comprise a light-permeable material to allow the microalgae to be exposed to light. If an artificial light source is used, the light source is The ML / t / zuzi / uai rus may be placed inside or outside the bioreactor, for example, in accordance with U.S. Patent No. 8,033,047, the full description of which is hereby incorporated by reference. Alternatively, in some embodiments, the system may comprise a water conduit through which water containing microalgae can be circulated in the bioreactor to expose the microalgae to light. Some embodiments may include the use of a water conduit adapted to employ sunlight, reflected light, curved light, fiber optic light, or artificial light. In some modes, the system can be run continuously, semi-continuously, or in a batch operation. In some embodiments, the system may further comprise one or more monitors or sensors adapted to monitor: a) growth conditions within the bioreactor; and / ob) microalgal cell titer / cell count in the water; and / oc) pH of the water; and / od) salinity of the water; and / oe) the presence of unwanted microbes in the bioreactor; and / of) water level; and / og) water pressure; and / oh) microalgal nutrient level; and / oi) solids level in the filtered water; and / oj) the level of unwanted compounds in the water; and / ok) oxygen, ozone and / or CO2 content in the water; and / o l) nitrogen compound level in the water; and / om) water clarity or opacity; and / on) level of desired compounds in the water; and / o) water flow rate; and / op) weedy algae; and / oq) algae predators; and / or other contaminants. In some embodiments, the monitor or sensors can be used to control system operation, such as through feedback regulation. In some embodiments, a monitor can generate one or more signals to controllers, which control the flow of materials into and / or out of the system. For example, in some embodiments, a microalgae cell count monitor can send one or more signals to one or more flow controllers indicating that the flow of source water or water containing microalgae is entering and / or leaving the system. In some embodiments, a pH monitor can send one or more signals to a CO2 flow controller that controls the amount or rate at which CO2 is added to the system. In some additional embodiments, a water level monitor can send one or more signals to a water flow controller that controls the amount or rate of water flow into and / or out of the system.In some configurations, a pH monitor can send one or more signals to an acid or base titration unit that controls the amount or rate of acid or base flowing into and / or out of the system. In some configurations, a water pressure monitor can send one or more signals to a water pressure regulator that controls the amount or rate of water flow into and / or out of the system. In some configurations, an ozone monitor can send one or more signals to an ozone flow controller that controls the amount or rate at which ozone is added to the system. In some additional configurations, a clarity monitor can send one or more signals to a water clarity controller that controls the water filtration efficiency in the system. In some other configurations, a nutrient monitor can send one or more signals to a nutrient source flow controller that controls the amount or rate at which the microalgae nutrient is added to the system. To grow, plants and microalgae need nutrients such as oxygen, carbon, nitrogen, phosphorus, potassium, magnesium, sulfur, boron, copper, chloride, iron, silicon, sodium, manganese, molybdenum, zinc, cobalt, vanadium, bismuth, iodine, water, carbon dioxide, air and / or others. The macronutrient and micronutrient profile provided by microalgae will depend on the strain or species used. Plants may require a different spectrum of micronutrients and macronutrients during different stages of their life cycle. Some methods for cultivating crops combine the macronutrient and micronutrient profile of microalgae with specific phases in a plant's life cycle. In some systems, a field can receive regular nutrient feeding during crop growth and development with different species used depending on the crop's needs.For example, microalgae A provide nutrient profile A, microalgae B provide nutrient profile B, and a target crop requires nutrient profile A during the early growth stages and a nutrient profile B during the later growth stages. In this situation, the soil in which the crop is planted will first be inoculated with microalgae A during the early growth stages of the target crop and then inoculated with microalgae B during the later growth stages of the target crop. Some embodiments include a method for producing a crop comprising: planting a crop in the soil and inoculating the soil with a first microalga that provides a first nutrient profile; and / or allowing the plant to progress from a first growth stage to a second growth stage; and / or inoculating the soil with a second microalga that provides a different second nutrient profile. In some embodiments, the first nutrient profile will be optimal for plant growth during the first stage, and the second nutrient profile will be optimal for plant growth during the second stage. Figure 1 represents a first embodiment of a portable microalgae-based soil inoculation system 1 of the invention. In some embodiments, the system comprises a water source 7, an ozone source 2, a carbon filter / UV light system 3, a water pump 8, a solids filter 9, a microalgae nutrient source 4a, 4b, bioreactors 6a, 6b, 6c, a carbon dioxide source 5, an air supply / pressurized air pump 10, and various water conduits. In some embodiments, the pressurized air supply may be a blower and / or air compressor and / or rocker pump and / or any other conventional producer or source of pressurized air. In some embodiments, the air is drawn from the atmosphere or a tank through the inlet 11, which optionally includes an air filter.In some embodiments, air is passed through air pump 10 to an ozone source 2, forming ozone-treated air, which is then piped to a water source 7 to form ozone-treated water. In some embodiments, air is also injected with a carbon dioxide source 5 to form carbon dioxide-treated air, which is piped to bioreactors 6a-6c or to the water entering the bioreactors. In some embodiments, the ozone-treated water is filtered through a solids filter 9, a carbon filter, and / or a UV light system 3 to form filtered water. Microalgae feed is added to this filtered water via microalgae feed sources 4a and 4b to form feed water, which is then piped to the bioreactor. In some embodiments, during initial startup, bioreactors 6a, 6b, and 6c are filled with water containing microalgae nutrients and then inoculated with a first inoculant containing microalgae.In some embodiments, air containing carbon dioxide is injected into the water containing microalgae in bioreactors 6a, 6b, and 6c. In some embodiments, the water in bioreactors 6a, 6b, and 6c is recirculated for a period of time until the microalgal cell count / titer has reached a target level suitable for use as an inoculant. In some embodiments, the water from system 1 is then flowed into the irrigation water to form a microalgal inoculant as the effluent, which is applied to the soil from an irrigation system 99. Several different operating parameters can be controlled. For example, in some configurations, one or more heaters are optionally included in the system to heat water circulated through the system and / or heat the culture medium in the bioreactor, thus enabling the cultivation of microalgae and the use of the system even during cold weather. In some embodiments, the system water volume and flow rate of the irrigation water in irrigation system 99 can be adjusted as needed to provide the appropriate level of inoculation and water penetration into the soil. For example, in some embodiments, a 200-acre (809,371 m²) field may receive a total daily volume of 500 to 1,000 gallons (1,892.71 to 3,785.41 L) of water at a distribution rate of approximately 21 gallons / hour (79.4 L / h) to 42 gallons / hour (158.9 L / h). In some embodiments, the inoculant obtained from the bioreactor (for example, such as one or more of bioreactors 6a, 6b, 6c) can be applied to the soil with or without further dilution. For example, in some configurations, system 1 can be operated so that all the water used for irrigation flows through the bioreactor.Otherwise, in some modalities, system 1 can be operated so that the inoculate, the effluent from bioreactors 6a, 6b, 6c, is diluted with additional irrigation water before application to the soil. In some bioreactors, the microalgal cell titer (cell count) fluctuates over time; therefore, the effluent cell titer also varies. The titer provides important metrics regarding the health and productivity of the unit. Generally, the effluent titer can range from at least 1,000,000 cells per ml to 30,000,000 cells per ml. The titer is also species-specific and may be higher or lower than the range indicated above. In some embodiments, ozone can be used to destroy unwanted microbes present in irrigation water before it enters the bioreactor. Any organic contaminants present in the system can be removed by ozonolysis as described in U.S. Patent No. 5,947,057 and U.S. Patent No. 5,732,654 to Perez et al. Organic contaminants include herbicides, pesticides, and fungicides, among others. In some embodiments, the ozone source can be an ozone generator. Ozone generators may include the Pacific Ozone Model 01, the Nano Absolute Ozone, and the Ozotech OZ8PC20. In some embodiments, the water is treated with ozone as required according to the quality of the water entering the system. In some embodiments, the ozone concentration in the water before filtration through a carbon filter will vary with the water quality but will have a sufficient ozone level to sterilize the water.In some forms, the treatment of water with ozone can be improved by using a mixer that blends the water and the ozone. In some configurations, carbon filters and UV light systems are used to remove ozone from irrigation water before it enters the bioreactor. In some configurations, the carbon filter typically uses a minimum of 0.75 ft³ (28.3 L) of activated carbon. In some configurations, the carbon filter and UV light systems are continuous flow systems. Suitable carbon filters in some configurations include the 0.75 ft³ (28.3 L) Upflow Carbon Filter System from Affordable Water (www.affordablewater.us). UV systems may include the Aquafine CSL Series and the UVS3XX Series from UV Sciences (www.aquaneuv.com; Valencia, CA). In some configurations, the UV light system can be used to disinfect water before it enters the bioreactor and / or to destroy ozone, chlorine, or chloramines before it enters the bioreactor. In some models, the UV light system can disinfect by inactivating or eliminating microorganisms in the water. In some configurations, when a solids filter is present, it can be used to remove solids from the irrigation water before it enters the bioreactor. In some configurations, the solids filter may be a continuous flow filter. In some configurations, suitable solids and filters may include the “X100” bag filter from www.filterbag.com or the “FV1” bag filter from www.aguaticeco.com. In some forms, suitable carbon and / or solid filters may include, but are not limited to, media filters, disc filters, grid filters, microporous ceramic filters, carbon block resin filters, membrane filters, ion exchange filters, microporous media filters, reverse osmosis filters, slow sand filter beds, fast sand filter beds, cloth filters and / or any other conventional filter. In some embodiments, carbon dioxide can be used as a carbon source for microalgae. In some embodiments, carbon dioxide can be added directly or indirectly to the bioreactor. In some embodiments, the carbon dioxide source can be a tank containing carbon dioxide, a carbon dioxide generator, a carbon dioxide sequestration device that captures carbon dioxide from the atmosphere, or a combination thereof. Alternatively, carbon dioxide captured from the air can be used, for example, U.S. Patent No. 8,083,836, the full description of which is hereby incorporated by reference. In other embodiments, carbon dioxide can be obtained from acetic acid and / or calcium carbonate. Atmospheric air contains approximately 0.035–0.04% carbon dioxide by weight. While atmospheric air can serve as a carbon dioxide source for microalgae, the carbon dioxide concentration is generally too low to sustain rapid microalgal growth in the bioreactor. Therefore, in some embodiments, carbon dioxide can be added to the air fed into the culture medium. In some embodiments, the carbon dioxide concentration in the air added to the culture medium is typically found in the range of approximately 1–3% by weight, 1.5–2.5% by weight, 1.8–<2.2% by weight, or approximately 2% by weight. In some configurations, a water pump may be included in the system. In some configurations, when present, the water pump can facilitate the flow of water through the system's water conduits and / or bioreactors. In some configurations, if a water pump is not included, the pressure of the incoming irrigation water may be sufficient to propel the water through the system. In some embodiments, an air pump or blower (these terms are used interchangeably herein) may be included in the system. In some embodiments, the air pump may facilitate airflow, which may or may not include carbon dioxide or ozone, through the system's air ducts, water source, and / or bioreactors. The size or operating capacity of each piece of equipment comprising the system can be varied as needed. For example, in some configurations, a portable system comprising a total bioreactor capacity of 500 gallons (1892.71 L) of culture medium can support 200 acres (809,371 m2) of land and will generally require the following minimum operating capacities for the indicated components: a) ozone source - 1.5 g / hr; ivia / t / zuz ι / υυ i ruó (dry air); b) solids filter - 40 g / min maximum flow with a minimum surface area of ​​2 square feet (0.185806 m2); c) carbon filter - 0.75 ft3 (28.3 L) minimum; d) water pump - 10 gal / min (37.8 L / min) minimum; e) pressurized air supply / air pump - 25 cfm at 60” H2O minimum; f) microalgae feed source - 1.0 x 106 cells / ml minimum; g) liquid carbon dioxide source - 80 1 / week. Figure 2 illustrates another embodiment comprising a portable system 51, wherein the system components 51 are mounted on a trailer. In some embodiments, the system 51 comprises a water tank 52, multiple bioreactors 53, an ozone generator 54, a clarifier 55, a combination filter / UV light system 56, a nutrient feed supply 57, a CO2 source 58, a pressurized air supply 59, and a trailer 60. As shown, any of the following can be mounted on the trailer 60: a water tank 52, a plurality of bioreactors 53, an ozone generator 54, a clarifier 55, a combination filter / UV light system 56, a nutrient feed supply 57, a CO2 source 58, and a pressurized air supply 59. In some embodiments, System 51 can accommodate a throughflow capacity of approximately 0.35–0.7 gal / min (1.3 L / min–2.6 L / min) and can be used to support a field in the range of 200–1000 acres (809,371 m²–4.04 km²). In some embodiments, Water Tank 52 can receive water from the water source on a farm site. In some embodiments, System 51 can comprise eight bioreactors (total capacity of 500 gallons (1892.71 L)), a water tank, air filter, solids filter, carbon filter, UV light system, ozone source, carbon dioxide source, microalgae nutrient source, pressurized air supply, and water pump (not shown). In some embodiments, the bioreactors 53 can have light-permeable walls so that sunlight is used as a light source.In some embodiments, carbon dioxide and air can be bubbled into the bottom of bioreactor 53 so that the bubbles agitate the culture medium as they rise. In some embodiments, system 51 optionally includes a mechanical agitator. In some embodiments, system 51 can deliver a minimum of approximately 800,000 microalgae cells per second through the effluent, assuming a water flow rate of approximately 0.35 gal / min (1.32 L / min). Figure 3 represents a side elevation view of another system 65 of the invention comprising a portable elevated platform 66, water tank 67, pressurized air supply 68, ozone source 69, clarifier 70, water filter 71, nutrient source 72, carbon dioxide source 73 and bioreactors 74. In some embodiments, one or more components can be mounted on the platform and one or more components can be placed on the ground or on one or more additional platforms. Although Figures 2 and 3 depict a water tank 52, 67 as the water supply, in other embodiments, a flowing water source may be used instead; therefore, in some embodiments, the system of the invention optionally includes one or more water tanks as the water supply or excludes a water tank as the water supply. Although not shown in Figures 2 and 3, in some embodiments, the effluent from one or more bioreactors may be fed into the water flow of an irrigation system. In some embodiments, the systems described herein may be placed within a partial or complete enclosure, even though the systems are portable. In some embodiments, the performance of the system in Figure 2 was evaluated in a crop study where melon crops were planted on 200 acres (809,371 m2) of land. The land was divided into control and sample sections (e.g., see Figures 5A–5B). The control sections received only irrigation water and were not treated with the microalgae supplement. The sample sections received only irrigation water containing the microalgae supplement. Melon seeds were planted before watering with the algae supplement in the soil. The control plants were watered approximately every four days, depending on the heat. The sample plants were irrigated on the same schedule as the controls. Several aspects of plant and fruit growth were evaluated for five weeks (shown in Figure 4A) and nine weeks (shown in Figure 4B) after planting. In short, the crop grown according to the systems and methods described herein produced larger and firmer plants. For example, compare Figure 5A (showing a control plant) with Figure 5B (showing a sample plant). Also, compare the larger melons in Figure 6B with the control plant shown in Figure 6A. Furthermore, the sample plants produced more flowers per vine, improved fruit texture and flavor, improved sugar content, improved nutritional content, improved appearance, and improved vitamin A content. Specific details and results are described in Example 1. In some modalities, the system may further comprise one or more monitoring devices to perform functions, including, but not limited to, measuring CO2 flow rate, CO2 content in the culture, O2 content in the culture, pH, cell density and temperature in the culture, measuring macronutrient content in the culture or effluent, measuring micronutrient content in the culture or effluent, or measuring the microalgae titer in the culture or effluent. Figure 7 represents an alternative embodiment of the system of the invention. In some embodiments, the system 11 is suitable for low, medium, and high-volume irrigation applications. In some embodiments, the system 11 comprises an optional pump 18 adapted to receive water from a pressurized or non-pressurized water source 11a. In some embodiments, the water received from the water source 11a is ozonated within an ozone contactor 12 that receives ozone from an ozone generator 27 and is directed to a clarifier / filter 19 that removes precipitated solids from the water. In some embodiments, after clarification, the water is directed to a carbon filter or UV light system 13, which removes the ozone, and through a mixer 22 that mixes the water with algae feed material obtained from the algae nutrient supply 14.In some embodiments, the algae / water mixture is blended using air bubbles, which are produced by a pressurized air supply 30 that delivers air to an air diffuser at the base of the bioreactor 16. In some embodiments, the water-containing nutrient material is piped into the bioreactor 16, where the microalgae are cultivated. In some embodiments, the effluent containing the microalgae exits the bioreactor 16 and passes through a valve 26 that regulates the water flow ratio between the bypass water source line 28 and the bioreactor effluent. In some embodiments, a controller 29 operates the valve 26 to achieve the desired flow volume ratio between the untreated source water (from the bypass line 28) and the effluent to provide an inoculant containing a desired or target microalgae titer. In some embodiments, system 11 may include one or more different controllers. For example, in some embodiments, controller 20 may comprise an optional feedback loop where water that has been improperly ozonated can be fed back into ozone contactor 12 for proper treatment. In some embodiments, controller 21 may comprise an optional feedback loop so that water that has not been sufficiently clarified can be fed back into clarifier 19 for proper clarification. In some embodiments, controller 23 may provide control over the supply of algae nutrients 14 to regulate the amount of feed material loaded into the water.In some embodiments, the controller 25, using a pH probe 24, can provide control over the carbon dioxide source 15 that loads carbon dioxide into the bioreactor 16 to regulate the carbon dioxide concentration in the water and ensure that the water has the appropriate carbon dioxide concentration. In some embodiments, the algae / water mixture can be mixed using air bubbles, which are produced by a pressurized air supply 30, which directs air to an air diffuser at the base of the bioreactor. In some embodiments, system 11 may comprise a portable platform (or body or frame, not shown) on which the plural system components are mounted. In some embodiments, each of the individual system components can be replaced individually. Although the components are listed as individual components, each component may be present in plurality independently of other system components. Figure 8 represents an alternative embodiment of the system of the invention. In some embodiments, the system 41, as shown, may be suitable for low, medium, and high-volume irrigation applications or for applications flowing into a distribution tank 37. In some embodiments, the distribution tank 37 may be mounted on a trailer for portability. In some embodiments, the system 41 comprises an optional pump 18 adapted to receive water from a pressurized or non-pressurized water source 11a. In some embodiments, the water from the water source 11a is ozonated within an ozone contactor 12 that receives ozone from an ozone generator 17. In some embodiments, the ozonated water is directed to a clarifier / filter 19 that removes precipitated solids from the water.In some embodiments, after clarification, the water is directed to a carbon filter or UV light system 13, which removes ozone, and then through a mixer 22 that blends the water with algae fertilizer / additives obtained from the algae nutrient supply 14. In some embodiments, the nutrient-containing water can be directed to the bioreactor 16, where microalgae can be cultivated. In some embodiments, the algae / water mixture can be blended using air bubbles, which are produced by supplying pressurized air 30, which directs air to an air diffuser at the base of the bioreactor as discussed earlier with respect to system 11 in Figure 7. In some embodiments, one or more probes 33 can be placed in the culture to measure critical parameters including pH, temperature, cell density, water mixing rate, gases, and dissolved nutrients.In some configurations, an optional telemetry device 34 can send probe metrics (monitoring devices or controllers) to a computer server for remote monitoring. In some configurations, an optional telemetry-capable microscope can assist with remote crop monitoring. In some configurations, the optional telemetry device 34 comprises the optional telemetry-capable microscope. As used herein, the telemetry device 34 may be any device capable of facilitating communication between the system of the invention and a remote control and / or communications center located in a different geographical location than the system of the invention. In some embodiments, the telemetry device 34 may employ any type of wireless communication system and may use any frequency of light waves, radio waves, sound waves, infrared waves, hypersonic waves, ultraviolet waves, other such wavelengths / frequencies, and combinations thereof. In some embodiments, the telemetry device 34 employs an IP network (such as the Internet), a GSM (Global System for Mobile Communications) network, an SMS (Short Message Service) network, other such systems, and combinations thereof. In some embodiments, a flow imaging device 32 can create images of the algae, predators, and contaminants in the culture for quality control (QC) purposes and can send this data to the telemetry device 34. In some embodiments, the effluent containing the microalgae can exit the bioreactor and pass through a valve 31 that regulates the flow of the bioreactor effluent. In some embodiments, the optional dewatering device 35 can concentrate the algae into a thick suspension of the desired density, which can flow into irrigation or portable vessels 37. In some embodiments, an optional microorganism mixer 36 can allow the user to mix the final product with, in addition to algae, beneficial bacteria, fungi, or other organisms 38 that function symbiotically with algae. In some embodiments, system 41 may include one or more different controllers. In some embodiments, controller 20 may comprise an optional feedback loop so that water that has been improperly ozonated can be fed back into the ozone contactor 12 for proper treatment. In some embodiments, controller 21 comprises an optional feedback loop so that water that has not been sufficiently clarified can be fed back into the clarifier 19 for proper clarification. In some embodiments, controller 23 provides control over the supply of algae nutrients 14 to regulate the amount of feed material loaded into the water.In some embodiments, the controller 25, by using a pH probe 24, can provide control over the carbon dioxide source 15 that loads carbon dioxide into the bioreactor in order to regulate the carbon dioxide concentration in the water and ensure that the water has the appropriate carbon dioxide concentration. In some embodiments, the system 11 may comprise a portable platform (or body or frame, not shown) on which the plural components of the system are mounted. In some embodiments, each of the individual components of the system can be replaced individually. Although the components are listed as individual components, each component may be present in plurality independently of other components of the system. In some applications, a system similar to System 41 in Figure 8 can be used to rehabilitate degraded or abandoned soil. In some applications, a mixture of algae and microorganisms produced by the system can be applied to the soil surface via irrigation or spraying to restore vital nutrients. The algae and other microorganisms continue to flourish in the soil as long as moisture is available. The algae supply micronutrients, attract other microorganisms, and add organic matter (humus) to the soil. In some applications, the process can rehabilitate degraded or abandoned soil. In some additional embodiments, a system similar to system 41 in figure 8 can cultivate other microorganisms in the same culture or separate containers to mix before the culture flows into the irrigation or portable containers. Figure 9 illustrates a soil enrichment system according to some additional embodiments of the invention. Some embodiments include a solids filter 919, a water storage tank 912, a sterilization system 917, and a neutralization system 915. A growth priming system may comprise one or more nutrient solution feeds, such as the first and second nutrient solution vessels 920, 962, for adding nutrient solutions to the treated water. A bioreactor system may comprise one or more bioreactors 916 for facilitating the inoculation and growth of the microorganism.Systems and methods may include several additional systems and subsystems, such as one or more nutrient solution containers, refrigerators, light sources, blowers (e.g., at least one pressurized air supply), carbon dioxide sources, pumps, valves, fluid ducts, air ducts, gas ducts, air filters, gas filters, control systems, sensors, air conditioning units, exhaust systems, portable accommodations and / or outdoor holding tanks. In some embodiments, one or more pumps 918, such as peristaltic pumps, may propel irrigation water from water source 95 through fluid conduits 910. Water source 95 supplies water to the soil enrichment system 900. The water flowing from water source 95 may be referred to as “irrigation water.” Water source 95 may comprise any suitable source of irrigation water appropriate for plant irrigation. In some embodiments, water source 95 may be pressurized, such as water from a well or a public utility in a city, town, or municipality. In some embodiments, water source 95 may be substantially unpressurized. For example, water source 95 may comprise a stationary water reservoir, reclaimed wastewater, well water, lake water, stream water, pond water, rainwater, river water, and / or freshwater. Some embodiments of the soil enrichment system 900 may comprise an automated cleaning system 970 controlled by a control system. The automated cleaning system 970 may comprise a cleaning solution vessel 968 for containing the cleaning solution and a pump 918 for pumping the cleaning solution from the cleaning solution vessel 968 into the fluid conduit and / or one or more bioreactors 916. In some embodiments, each of the one or more bioreactors 916 may comprise a dedicated valve for connecting a fluid conduit leading to the cleaning solution vessel 968. In some embodiments, one or more bioreactors 916 can be inoculated with the microorganism inoculant by any suitable method, such as manual inoculation through a port 935 in the bioreactor 916. In some additional embodiments, neutralized irrigation water containing nutrient solution can be conveyed into any one or more of the bioreactors 916 until it reaches a preselected fill level 940. In some embodiments, a light source 945 / 950 can be configured to project light onto and / or into each of one or more bioreactors 916. In some embodiments, the light source 945 / 950 can comprise LED lights in any configuration suitable for providing light to the microorganism culture. For example, in one embodiment, a first light source 945 can be placed inside one or more bioreactors 916. In another embodiment, the first light source 945 can be superimposed on an exterior surface of one or more bioreactors 916. In another embodiment, a second light source 950 can be outside and adjacent to an exterior surface of one or more bioreactors 916. In some embodiments, a control system suitable for implementing one or more of the herein embodiments may include a computer system communicatively linked to a PLC system 934. The PLC system 934 may be communicatively linked to one or more sensors 933 and may provide measurements obtained by the one or more sensors 933 to a processor and / or database for remote monitoring, remote data access, and / or remote control of the soil enrichment system 900. Similarly, the PLC system 934 may be communicatively linked and configured to control pumps 918, valves, a sterilization system 917, a neutralization system 915, at least one pressurized air supply 930, lights 950, and / or any carbon dioxide source. In some embodiments, a carbon dioxide source 966 can be used to supply a carbon source to the microorganism culture. The carbon dioxide can be added directly and / or indirectly to one or more bioreactors. The carbon dioxide source 966 can be a tank containing carbon dioxide gas, a carbon dioxide generator, a carbon dioxide sequestering system for capturing and temporarily storing atmospheric carbon dioxide, or a combination thereof. In some embodiments, the microorganism culture can be released from one or more bioreactors 916 through outlets, flow through one or more fluid conduits, and flow into the external holding tank 937 for storage. In various embodiments, the external holding tank 937 may comprise a material that is at least partially transparent, such as polyethylene, polycarbonate, acrylic, and / or high- or low-density PVC, to allow natural or artificial light to penetrate through the external holding tank 937 and into the microorganism culture. In some embodiments, the external holding tank 937 may comprise a sterile aeration system to support the health of the microorganism culture. In some embodiments, the external holding tank 937 may comprise a cone-shaped base to ensure complete drainage of the microorganism culture when released into a target field 955. In some embodiments, the 937 external holding tank may include a cooling system, such as a refrigerator, for cooling the microorganism culture during storage. The refrigerated external holding tank can be configured to receive the microorganism culture and / or the thick microorganism suspension, maintain its sterility, and store it at any suitable temperature. In some embodiments, the dewatering device 964 can be configured to deliver the concentrated thick microorganism suspension to the target field 955 and / or the external holding tank 937. The dewatering device 964 can concentrate the microorganism culture through any suitable process such as, but not limited to: 1) flocculation and sedimentation; 2) flotation and collection; and / or 3) centrifugation. Additional details and operating characteristics of the soil enrichment system 900 are described in U.S. Patent Application No. 15 / 647,005, the full contents of which are incorporated herein by reference. Some embodiments include methods for isolating, selecting, and using endemic microbes for agricultural production areas using any of the systems described herein. For example, some embodiments of the invention include methods for selecting, collecting, and cultivating algae for distribution to an agricultural production area. Specifically, in some embodiments, the methods focus on collecting, isolating, and / or propagating endemic microbes, primarily algae, for mass distribution to the same biome from which the algae were collected. In some embodiments, the agricultural production area comprising the biome may be a crop field, raised bed, greenhouse, golf course, degraded soil, or an indoor cultivation facility. Additional embodiments include collecting, isolating, and / or propagating and distributing other endemic microbes in addition to, or separately from, the algae.For example, some methods include collecting, isolating, and / or propagating and distributing a bacterial species. Other methods include collecting, isolating, and / or propagating and distributing a fungal species. In some embodiments of the invention, the algae can be distributed through a variety of means, including, but not limited to, canal irrigation, flood irrigation, drip irrigation, various conventional aerial spraying techniques, and / or various conventional hydroponic cultivation techniques. In some embodiments of the invention, the effects of distributing algae to the agricultural production area may be an increase in soil organic matter, an improvement in soil structure, a reduction in water and fertilizer use, and / or an increase in crop yield. MA / 1 / u» 1 rus the nutritional value of the product, and / or an overall improvement in soil health, and / or a reduction in water and chemical runoff, and / or an increase in carbon dioxide sequestered from the air by the soil. Some embodiments of the invention include a method for obtaining a soil and / or water sample from an agricultural production area and / or cultivating microbes from the soil sample and / or selecting a desirable species from the soil sample and / or propagating the selected desirable species in greater numbers and concentration and / or distributing live microbes to the agricultural production area (e.g., such as dispersing the live microbes in solution over a soil area of ​​a farm or biome area). The following steps constitute a non-limiting modality of a method for collecting, selecting, and propagating algae endemic to an agricultural production area (e.g., such as a farm or other plant propagation facility): Some methods include a step of collecting one or more quantities of soil from one or more locations within the agricultural production area. In some methods, each quantity or the total quantity of soil collected may be approximately 100 grams. In other methods, the quantity may be less than 100 grams or greater than 100 grams. Some methods include a step of collecting one or more quantities of water from one or more locations within the agricultural production area (for example, from a surface water source). In some methods, each quantity or the total amount of water collected may be approximately 50 grams. In some other methods, the quantity may be less than 50 grams or greater than 50 grams. In some other methods, at least some of the water may be collected from a groundwater source, a runoff source, or a well or spring source. In some modalities, one or more of the quantities of water and / or soil may be cooled to 35°F to 40°F (1.6°C to 4.4°C) prior to further processing locations, including, without limitation, a laboratory or facility. In some methods, approximately 10 grams of soil or 10 ml of water from each sample can be added to a 100 ml culture flask containing 75 ml of medium. AF6 (Watanabe). Culture flask. AF6 (Watanabe). In some variations, more or less soil and / or water may be added. In some additional variations, more or less media may be used. In some variations, the soil and / or water may be incubated in the culture flask. In some variations, incubation may occur overnight while exposed to a light source of 100 to 200 PAR. In some variations, the light source may comprise or emit wavelengths of approximately 450 nm to 485 nm and / or approximately 625 nm to 740 nm. In some variations, exposure may be approximately 12 to 24 hours per day. In some embodiments, a portion of the incubated samples can be spread onto agar-coated petri dishes. For example, in one non-limiting embodiment, the samples can be placed in four 100 x 15 mm AF6 agar petri dishes with 10 µL samples, with loop sterilization between each vein to dilute the sample. In some embodiments of the invention, the petri dishes can be at least partially sealed (e.g., taped to a 75% seal) and placed upside down in front of a 100 to 200 PAR light source for one to two weeks. In some embodiments, the light source can comprise or emit wavelengths of approximately 450 nm to 485 nm and approximately 625 nm to 740 nm. In some embodiments, the exposure can be approximately 12 to 24 hours per day. In some formulations, once isolated axenic algal colonies have grown to a specific size, they can be aseptically collected and placed in a sterile test tube containing sterile AF6 medium. For example, in some formulations, once isolated axenic algal colonies have grown to approximately 3 mm in diameter, they can be aseptically collected and placed in a sterile test tube containing sterile AF6 medium. Some methods may include an incubation period of one to two weeks, followed by the selection of tubes with the highest biomass. In some methods, incubation may occur under exposure to a 100 to 200 PAR light source. In some additional methods, the light source may contain wavelengths of approximately 450 nm to 485 nm and approximately 625 nm to 740 nm. In some methods, exposure may be approximately 12 to 24 hours per day. In some additional methods, temperatures may range from approximately 70 °F to 80 °F (21.1 °C to 26.6 °C). Some methods include subculturing each tube into a new tube, followed by placing the contents of the original tube into a sterile 500 ml bottle containing AF6 medium equipped with sterile air injection. In some methods, the subculture tubes may be exposed to a 100–200 PAR light source. In some methods, the light source may contain wavelengths of approximately 450–485 nm and 625–740 nm. In some methods, the exposure may be approximately 12–24 hours per day. Some methods include incubating the bottle for 3–5 days, selecting bottles with the fastest growth rate and highest biomass, and assigning them a new strain ID. In some methods, incubation may occur under a 100–200 PAR light source. The light source may have wavelengths of approximately 450–485 nm and 625–740 nm. Exposure may be approximately 12–24 hours per day. Temperatures may range from approximately 70–80 °F (21.1–26.6 °C). In some embodiments of the invention, the strain IDs of the incubated samples can be recorded in the strain ID database along with the date, time, and location of collection, and any additional algal characteristics. Furthermore, in some embodiments, new test tubes can be inoculated with each newly identified strain and placed in the algal library. In some embodiments of the invention, an additional step may include an artificial selection process to improve growth rate, maximum density, and other desired characteristics. In some embodiments, the artificial selection process may involve exposing algal strains to preferred cultivation conditions. In some embodiments, algal strains with improved growth rate, higher maximum density, or other desired characteristics may be selected over inferior strains for future use. In some embodiments, the inferior algal strains may be subjected to the artificial selection process to further improve growth rate, maximum density, or other desired characteristics. In some embodiments of the invention, one or more of the steps can be performed in a laboratory or facility located away from the agricultural production area. In some embodiments of the invention, one or more of the steps can be performed in a laboratory or facility located near or part of the agricultural production area. In some embodiments, all the steps can be performed at the same location. In other embodiments, at least some of the steps can be performed at one location, and one or more additional steps can be performed at another location. In view of the foregoing description and the examples below, a person skilled in the art will be able to practice the invention as claimed without undue experimentation. The foregoing will be better understood with reference to the following examples. All references to these examples are for illustrative purposes. The following examples should not be considered exhaustive, but merely illustrative of only a few of the many embodiments contemplated by the present invention. Example 1 Evaluation of the Melon Growth System The system of the invention was used to cultivate the Yosemite variety of cantaloupe melons. Approximately 200 acres (809,371 m²) were infused with irrigation water containing microalgae. The crop was irrigated every five days in the evenings due to the high ambient temperatures (120°F (48.8°C)). Microalgae were added to the irrigation water continuously with each irrigation. Algae from the phyla Chlorophyta and Cyanophyta were added to the irrigation water at a combined density of 6 billion cells per minute. The algae were cultivated in the media shown in the table below. FW Media Final Concentration (g / L) Standard Solutions (g / L) Usage Rate (ml / L) N&P Solution NaNO3 0.344 34.4 10 KCl 0.303 30.3 NaH2PO4 0.03 2.91 Solutions of Missing Elements CaCl2-2H2O 0.11 11 10 MgSO4-7H2O 0.246 24.6 Trace Element Solution Na2EDTA-2H2O 0.0045 4.5000 1 FeCl3'6H2O 0.00289 2.8910 MnCl2-4H2O 0.00098 0.9800 ZnSO4*7H2O 0.000036 0.0360 CoCl2-6H2O 0.000011 0.0110 Na2MoO4-2H2O 0.00012 0.1200 CrO3 0.000075 0.0750 SeO2 0.000005 0.0050 CuSO4'5H2O 0.000012 0.0120 Vitamins Biotin 0.000025 0.025 1 Thiamine HCl (substance) 0.0000175 0.017 B12 0.000015 0.015 The melons were harvested and the following observations were made when comparing melons grown according to the invention with melons not grown according to the invention. Metric Description Productivity Melon production improved by 20% in weight. Size The fruit increased in diameter by 22%. Texture: The fruit's flesh texture was retained or improved. Shelf Life: The shelf life was extended by 4 days. Flavor: The fruit's flavor was retained or improved. Sugar: The fruit's sweetness improved by 20%. Appearance: The fruit's appearance and color were retained or improved. Vitamin A: The vitamin content improved by 20%. Several different dimensions of melon plants were measured 9 weeks after planting for both control plants and plants grown using the system of the invention. The observed dimensions are detailed below. Parameter Control Sample Multiplication Factor Trunk Diameter 0.129 in. (0.32 cm) 0.38 in. (0.96 cm) 2.9 Stem Diameter 0.05 in. (0.127 cm) 0.125 in. (0.31 cm) 2.5 Average Leaf Length 2.5 in. (6.35 cm) 4 in. (10.16 cm) 1.6 Length of Largest Leaf 3.5 in. (8.89 cm) 7 in. (17.78 cm) 2.0 Total Plant Radius 37.8 in. (96 cm) 87.12 in. (221.28 cm) 2.3 Total Plant Height 5.7 in. (14.47 cm) 15 in. (38.1 cm) 2.6 Flower Size Width 0.9 in. (2.286 cm) 2.3 in. (5.842 cm) 2.6 Melon diameter 2.3 in. (5.842 cm) 5.5 in. (13.97 cm) 2.4 Melon fields infused with algae required 50% less inorganic nitrogen fertilizer and 40% less phosphorus and potassium. Micronutrient savings were on the order of 70%. The farmer reported a fivefold improvement in soil porosity, allowing for deeper root systems. The increased soil porosity also allowed symbiotic macro- and microorganisms, such as earthworms, to enter the field. The farmer reported that the melon fields required more than 50% less pesticide application because the algae-infused crops appeared to produce their own biopesticides that discouraged invaders, such as the whiteflies that were destroying neighboring fields. The farmer used 70% less fungicide because the algae allowed for longer roots that were more resistant to nematodes and other soil pests.Therefore, the system of the invention provides substantial improvements in the characteristics of the plants and fruits cultivated with the system of the invention. Example 2 Culture Growth Using Two Different Microalgae Before planting crop seeds in the soil, the soil is repeatedly irrigated with an inoculant containing a first species of microalgae from the phylum Chlorophyta until the soil has achieved the desired properties of increased organic matter and polysaccharides to enhance water retention. The seeds are then planted in the treated soil and irrigated repeatedly with a second inoculant containing a different species of microalgae from the phylum Cyanophyta to infuse the soil with nitrogen sequestered from the atmosphere until the crop reaches maturity. The crop is then harvested using established methods. At this point, a third species, also from the phylum Cyanophyta, is introduced into the irrigation water and applied to the soil, where it produces a biological toxin to kill unwanted soil pests.The first species of microalgae from the phylum Chlorophyta is used to improve soil fertility and other properties by increasing organic matter in the soil. This enhances colonization by other micro- and macroorganisms, which further improve the soil by converting nutrients into forms more readily available to crops and by increasing soil porosity. The second species of microalgae from the phylum Cyanophyta is used to add nitrogen to the soil, thereby reducing the amount of nitrogen fertilizer needed for cultivation. The third species of Cyanophyta is used to eliminate or reduce the number of soil pests. Example 3 System that Employs Co-Cultivation of Two Different Microalgae A system containing a co-culture of two different microalgae strains is prepared by creating a culture medium in one or more bioreactors and inoculating it with one or more blue-green algae (cyanobacteria or Cyanophyta) and one or more green algae (Chlorophyta). Both algae can be either unicellular or colonial; however, unicellular species are preferred. Some Chlorophyta include those of the class Chlorophyceae, which includes those of the orders Chaetopeltidales, Chaetophorales, Chlamydomonadales, Chlorococcales, Chlorocystidales, Dunaliella, Microsporales, Oedogoniales, Phaeophilales, Sphaeropleales, Tetrasporales, and Volvocales. Some Chlorophyta species include Chlorella fusca, Chlorella zofingiensis, Chlorella spp., Chlorococcum citriforme, Chlorella stigmataphora, Chlorella vulgaris, Chlorella pyrenoidosa, and others. Some Cyanophyta include those of the orders Chroococcales, Gloeobe, Nostocales, Oscillatoriales, Pseudanabaenales, and Synechococcales. The algae are co-cultured under natural and / or artificial light. The algal count in the culture medium is allowed to increase to a target level of approximately 1 to 100 million cells per milliliter. The culture medium is then discharged from the bioreactor and mixed with water for irrigation. As used herein and unless otherwise specified, the term “approximately” or “around” is deemed to mean ±0.10%, ±0.5%, ±0.2.5%, or ±0.1% of a specified value. As used herein and unless otherwise specified, the term “substantially” is understood to mean “largely,” “at least a majority of,” “more than 70%,” “more than 85%,” “more than 90%,” “more than 95%,” “more than 98%,” or “more than 99%.” The foregoing is a detailed description of particular embodiments of the invention. It will be appreciated that, although specific embodiments of the invention have been described herein for illustrative purposes, various modifications may be made without departing from the spirit and scope of the invention. Accordingly, the invention is not limited except by the appended claims. All embodiments described and claimed herein may be realized and implemented without undue experimentation, taking into account the present description. Those skilled in the art will appreciate that, although the invention has been described above in relation to particular embodiments and examples, it is not necessarily so limited, and that it is intended that many other embodiments, examples, uses, modifications, and deviations from the embodiments, examples, and uses are encompassed by the appended claims herein. The full description of each patent and publication cited herein is incorporated by reference, as if each of these patents or publications were individually incorporated by reference herein. Several features and advantages of the invention are set forth in the following claims.

Claims

1. A cultivation system, comprising: a bioreactor adapted for propagating microalgae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient comprising at least one carbon source, wherein the microalgae are freely suspended in and form part of the culture solution; and a water conditioning assembly; an algal nutrient supply coupled to the bioreactor;a first controller configured to control the flow of fluids between the water conditioning assembly and the bioreactor, the water conditioning assembly coupled as a water supply inlet to the bioreactor and configured to condition the water supply to a specific purity that allows substantially unhindered growth of microalgae in the culture solution at a specific microalgae concentration, and wherein the first controller is configured to control the supply of algal nutrients to the bioreactor; and a carbon dioxide source coupled to the bioreactor, wherein carbon dioxide is injected into the culture solution as the carbon source.

2. The system of claim 1, further comprising a second controller coupled to a probe, the second controller being configured to regulate the release of carbon dioxide from the carbon dioxide source to the bioreactor based at least in part on one or more measurements from the probe.

3. The system of claim 2, wherein the probe is a pH probe configured to measure a pH of the culture solution.

4. The system of claim 1, wherein the water conditioning assembly includes an ozone generator coupled to an ozone contactor, wherein the ozone generator is configured to generate ozone and distribute the ozone to at least partially ozonize the supply water.

5. The system of claim 4, further comprising a solids filter upstream of the ozone contactor.

6. The system of claim 5, further comprising a carbon filter and / or a UV light system positioned downstream of the solids filter, wherein at least one of the carbon filter and the UV light system are configured and arranged to at least partially deozonize the ozonated supply water.

7. The system of claim 1, further comprising at least one pressurized air supply coupled to the bioreactor, wherein the at least one pressurized air supply is configured to generate gas bubbles to aerate and / or at least partially agitate the culture solution.

8. The system of claim 7, wherein the gas bubbles include at least one of CO2, N2 and O2.

9. The system of claim 1, further comprising at least one water reservoir or tank that provides or is coupled to the supply water inlet.

10. The system of claim 1, further comprising a mobile trailer supporting at least the bioreactor, the water conditioning assembly, and the carbon dioxide source.

11. The system of claim 1, wherein the microalgae nutrient supply comprises at least one of a fertilizer, a macronutrient, a micronutrient, and at least two different microalgae species; and wherein the macronutrient is selected from the group consisting of phosphorus, nitrogen, carbon, silicon, calcium salt, magnesium salt, sodium salt, potassium salt, and sulfur; and the one or more micronutrients are selected from the group consisting of manganese, copper, zinc, cobalt, molybdenum, vitamins, and trace elements; and wherein the micronutrient comprises at least one of a vitamin and a mineral added to the conditioned supply water.

12. The system of claim 1, further comprising a telemetry system configured for at least one remote monitoring and control operation of one or more of the first controller, the second controller, the bioreactor, and at least one component or assembly of the water conditioning assembly.

13. The system of claim 1, wherein the artificial light comprises LED lights placed at least one inside the bioreactor and / or near an outer surface of the bioreactor and exposing the microalgae to light.

14. The system of claim 1, wherein the carbon dioxide source comprises at least one of a tank comprising liquid or gaseous carbon dioxide, a carbon dioxide generator, and a carbon dioxide sequester that sequesters and temporarily stores atmospheric carbon dioxide.

15. The system of claim 1, wherein the supply of algae comprises at least one of a first type of algae and a second type of algae.

16. The system of claim 1, further comprising a flow imaging device coupled to a bioreactor outlet, wherein the flow imaging device is configured to create images of at least one type of algae, predators, and contaminants in the culture solution for quality control monitoring. ma / e / zuzi / uai rus 17. The system of claim 1, further comprising a microorganism mixer configured to mix at least one of algae, bacteria, virus, and fungi with any of the culture solution exiting the bioreactor.

18. A method comprising: preparing one or more samples containing microbes from at least one location within an actual or planned plant growing area; preparing at least one cultured sample by culturing microbes from the sample; selecting at least one target microbe species from the at least one cultured sample; propagating the selected at least one target microbe species to increase the concentration of the at least one target microbe species in the at least one cultured sample by: providing a bioreactor adapted to propagate the selected at least one target species in a culture solution, the selected at least one target species being freely suspended in and forming part of the culture solution;coupling a power supply to the bioreactor and a first controller to control the flow between a water conditioning assembly and the bioreactor, the water conditioning assembly coupled as a water supply inlet to the bioreactor to condition the water supply to a specific purity that permits substantially unhindered growth of at least one selected target species in the culture solution at a specific concentration, and wherein the first controller controls the supply of the power supply to the bioreactor; and providing a carbon dioxide source coupled to the bioreactor, and regulating the release of carbon dioxide from the carbon dioxide source to the bioreactor, wherein the carbon dioxide is injected into the culture solution as a carbon source that permits propagation of at least one selected target species of microbe.

19. The method of claim 18, further comprising a second controller coupled to a probe and the bioreactor, wherein the second controller regulates the release of carbon dioxide from the carbon dioxide source to the bioreactor.

20. The method of claim 18, further comprising distributing at least a portion of the at least one target species of microbe to at least a portion of the at least one location.

21. The method of claim 20, wherein at least a portion of the at least one target species of microbe being distributed comprises at least one live microbe.

22. The method of claim 21, wherein the at least one living microbe is a species of algae endemic to the distribution location.

23. The method of claim 21, wherein the at least one live microbe is a live species selected to restore a normal soil flora mixture of farmland.

24. The method of claim 23, wherein the live species of algae are selected for their specific properties desired to improve the soil at the distribution location.

25. The method of claim 18, wherein the water conditioning assembly includes an ozone generator coupled to an ozone contactor, wherein the ozone generator generates ozone and distributes the ozone to at least partially ozonize the supply water.

26. The method according to claim 25, further comprising positioning a solids filter upstream of the ozone contactor.

27. The method of claim 26, wherein at least one carbon filter and / or UV light system is placed downstream of the solids filter, wherein the at least one carbon filter and UV light system at least partially deozonizes the ozonated supply water; and at least one pressurized air supply coupled to the bioreactor, wherein the at least one pressurized air supply generates gas bubbles to aerate and / or at least partially agitate the culture solution in the bioreactor.

28. A method comprising: sampling algal flora from an agricultural location; selecting at least one desired algal species for propagation from the algal flora, the at least one desired algal species being present in the algal flora of the agricultural location at an initial concentration; propagating the at least one desired algal species in at least one bioreactor; and distributing the at least one desired species to the agricultural location to increase the concentration of the algal species to a concentration higher than the initial concentration.

29. The method of claim 28, wherein the at least one bioreactor is adapted to propagate the at least one desired species of algae in a culture solution using in combination at least one of natural and artificial light, and at least one nutrient comprising at least one carbon source, wherein the at least one desired species of algae is freely suspended in and forms part of the culture solution;and an algal nutrient supply coupled to at least one bioreactor and a controller for controlling the flow between a water conditioning assembly and the at least one bioreactor, the water conditioning assembly coupled as a water supply inlet to the at least one bioreactor, and conditions the water supply to a specific purity that permits substantially unhindered growth of the at least one desired algal species in the culture solution at a specific concentration, and the controller controls the algal nutrient supply to the supply to the at least one bioreactor; and a carbon dioxide source coupled to the at least one bioreactor, wherein the carbon dioxide is injected into the culture solution as the carbon source.

30. The method of claim 29, further comprising a second controller coupled to a probe, wherein the second controller regulates the release of carbon dioxide from the carbon dioxide source to the bioreactor based at least in part on one or more measurements from the probe.

31. The method of claim 29, wherein the water conditioning assembly includes an ozone generator coupled to an ozone contactor, wherein the ozone generator generates ozone and distributes the ozone to at least partially ozonize the supply water.

32. The method of claim 29, wherein a solids filter is placed upstream of the ozone contactor, wherein the solids filter removes solids from the supply water.

33. The method of claim 29, wherein at least one of a carbon filter and / or a UV light system is placed downstream of the solids filter, wherein at least one of the carbon filter and the UV light system at least partially deozonizes the ozonated supply water.

34. The method of claim 33, wherein at least one pressurized air supply is coupled to the bioreactor, wherein the at least one pressurized air supply generates gas bubbles to aerate and / or at least partially agitate the culture solution in the at least one bioreactor.