Method and composition matter for immunoproteasome-mediated delivery into living cells

A molecular construct with a cell-penetrating moiety selectively targets immunoproteasomes in living cells, addressing the limitations of existing probes by enabling selective delivery and monitoring of cargo for diagnostic and therapeutic purposes.

US12449422B2Active Publication Date: 2025-10-21PURDUE RES FOUND
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Patent Information

Application Number
US17/428676
Authority / Receiving Office
US · United States
Patent Type
Patents(United States)
Current Assignee / Owner
Priority Date
2019-02-05
Filing Date
2020-01-29
Publication Date
2025-10-21
Estimated Expiration
2042-08-30

AI Technical Summary

Technical Problem

Existing probes for monitoring and delivering cargo to immunoproteasomes in living cells suffer from poor selectivity and inability to cross the cell membrane, limiting their use to in vitro assays and preventing effective diagnostic and therapeutic applications.

Method used

A molecular construct comprising a cargo linked to a moiety that penetrates the cell membrane and is selectively recognized by immunoproteasomes, allowing for the delivery of diagnostic or therapeutic agents, such as fluorescent probes, to diseased cells overproducing immunoproteasomes.

Benefits of technology

The construct achieves selective delivery and monitoring of immunoproteasome activity in living cells, enhancing diagnostic and therapeutic capabilities by maintaining probe selectivity and cell permeability, enabling real-time monitoring and targeted cargo release in diseased cells.

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Patent Text Reader

Abstract

The present invention generally relates to methods and composition matters for delivery of a cargo to a living cell over producing immunoproteasomes. More specifically the invention disclosed is a molecular construct that delivers a cargo, for diagnostic or therapeutic purposes, to a living cell. Methods of use and composition matters are within the scope of this disclosure.
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