Adult mouse tail tissue sampling device

Abstract

An adult mouse tail tissue sampling device, comprising a base (1), wherein a fixed assembly is fixedly mounted on the base (1), and the fixed assembly is a cover plate (2) having a conical surface structure; a cavity is formed between the cover plate (2) and the base (1); the cover plate (2) has an open end with a large cross section; a slit (3) is provided in the cover plate (2), and the slit (3) extends from the open end toward the other end of the cover plate (2); and an object placement platform (4) is provided on the side of the base (1) away from the open end of the cover plate (2), a fixing plate (5) is provided on the object placement platform (4), and a plurality of holes are provided in the fixing plate (5). The provided adult mouse tail tissue sampling device can physically isolate mice, prevent sampling staff members from being bitten, and also improve the sampling efficiency.

Description

Adult mouse tail tissue sampling device Technical Field

[0001] The present invention mainly relates to the technical field of life science research, and specifically relates to a device for sampling tail tissue of adult mice. Background Art

[0002] During the genetic selection experiment of gene-modified offspring mice after breeding of adult mice, there will be a stage of sampling of experimental animal tissues. In most cases, the time of animal sampling is about 10 days after birth. However, due to reasons such as sample loss, genome contamination, gene typing failure, and genotype confirmation before biological purification, it is necessary to sample the tail tissue of the animal after weaning. At this time, the animal is close to adulthood or has reached adulthood.

[0003] Generally, scientific researchers will grab an adult mouse with one hand and use the other hand to cut the tail to take a sample, place the tissue sample into a centrifuge tube, and disinfect the tail. This may lead to low sampling efficiency and the risk of being bitten by the animal. Technical issues

[0004] The present invention mainly provides an adult mouse tail tissue sampling device, which can physically isolate the mice, avoid the sampling personnel from being bitten, and improve the sampling efficiency. Technical Solutions

[0005] The technical solution adopted by the present invention to solve the above technical problems is:

[0006] A device for sampling tail tissue of adult mice, which is special in that:

[0007] The invention comprises a base, on which a fixing assembly is fixedly mounted, wherein the fixing assembly is a cover plate with a conical surface structure, a cavity is formed between the cover plate and the base, the cover plate has a larger cross-section and an open end, and a gap is provided on the cover plate, and the gap extends from the open end to the other end of the cover plate;

[0008] A storage platform is provided on one side of the base away from the opening end of the cover plate. A fixing plate is provided on the storage platform. A plurality of holes are provided on the fixing plate.

[0009] Furthermore, two columns are provided on the base, and the two columns are respectively located on both sides of the opening end of the cover plate. A sliding groove is opened opposite to each other in the two columns, and a movable plate is provided between the two columns. Sliders are provided on both sides of the movable plate. The movable plate moves up and down in the sliding groove through the sliders on both sides. The movable plate is used to seal the opening end of the cover plate. When the movable plate slides to the lowermost end, the opening end of the cover plate is just sealed.

[0010] Furthermore, a connecting plate is installed at the top of the movable plate, and first through holes are opened on both sides of the top of the connecting plate. Locking bolts are installed on the first through holes, and the connecting plate is fixedly connected to the movable plate through the locking bolts.

[0011] A first threaded hole is provided in the middle of the connecting plate, a screw is installed on the first threaded hole, and a handwheel is fixedly installed on the upper end of the screw; a second through hole is provided in the middle of the movable plate, the screw passes through the second through hole, and a second threaded hole is provided on the base corresponding to the second through hole.

[0012] When the movable plate slides to the lowermost end, the open end of the cover plate is sealed. At this time, the hand wheel is turned to make the screw enter the second threaded hole on the base to fix the movable plate on the base to prevent mice from escaping.

[0013] Furthermore, the base is a stainless steel flat plate, and the columns are made of stainless steel, so as to prevent adult mice from biting or scratching the base to damage it.

[0014] Furthermore, the cover is a transparent cover made of transparent plastic material, which makes it convenient for operators to observe the status of adult mice.

[0015] Furthermore, the movable plate is a fan-shaped plate structure.

[0016] Furthermore, the connecting plate is a curved plate structure, and two sides thereof are in contact with the fan-shaped outer side surfaces of the movable plate.

[0017] Furthermore, the starting and ending ends of the slits on the cover plate are rounded to prevent sharp edges from scratching mice, thereby ensuring animal welfare.

[0018] Furthermore, the columns are welded to the base. Beneficial effects

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) The present invention provides a device for sampling tail tissue of adult mice. When sampling, researchers only need to gently pull the tail of the animal with their hands to make the tail pass through the gap and the mouse body enter the cover, so that the other parts of the animal body are completely physically isolated from the researchers. There is no need to perform complicated animal restraint operations. Whether it is a skilled researcher or a novice researcher, they can operate it alone to complete safe and efficient tail tissue sampling. This device is easy to operate and can greatly improve the efficiency of single mouse tail tissue sampling, reduce the risk of researchers being bitten, and ensure animal welfare.

[0021] (2) The adult mouse tail tissue sampling device of the present invention has a movable plate that can block the outlet of the cover plate to prevent the mouse from escaping. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG1 is an overall structural diagram of a mouse tail tissue sampling device provided by the present invention;

[0023] FIG2 is an overall structural diagram of another mouse tail tissue sampling device provided by the present invention;

[0024] FIG3 is a structural diagram of the mouse tail tissue sampling device in FIG2 when the movable plate is opened.

[0025] In the figure: 1. Base; 2. Cover; 3. Gap; 4. Storage table; 5. Fixed plate; 6. Column; 7. Slide; 8. Movable plate; 9. Connecting plate; 10. Centrifuge tube; 11. Locking bolt; 12. Screw; 13. Handwheel; 14. Second threaded hole. Modes for Carrying Out the Invention

[0026] To facilitate understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the content disclosed in the present invention more thorough and comprehensive.

[0027] Example 1

[0028] Referring to Figure 1, a device for sampling tail tissue from an adult mouse is shown. The device comprises a base 1, to which is fixedly mounted a fixing assembly, comprising a cover plate 2 having a quarter-conical cylindrical structure, forming a cavity between the cover plate 2 and the base 1. The cover plate 2 has a larger cross-section and is open at one end. A slit 3 is defined directly above the cover plate 2, extending from the open end to the other end of the cover plate 2.

[0029] A storage platform 4 is provided on one side of the base 1 away from the open end of the cover 2 . A fixing plate 5 is provided on the storage platform 4 . The fixing plate 5 is provided with a plurality of holes for fixing centrifuge tubes 10 for collecting tissue samples.

[0030] The specific operation of this embodiment is as follows:

[0031] First, when the sampling personnel need to sample the mouse tail, a fixed plate 5 is placed on the storage table 4, and a centrifuge tube 10 is placed in the hole on the fixed plate 5. Then the scientific researcher only needs to gently pull the animal's tail with one hand, drag the mouse into the open end of the cover plate 2, so that the tail passes through the gap, and the mouse body enters the cover plate upside down, so that the other parts of the animal's body are completely physically isolated from the scientific researcher. At this time, the tail is cut with the other hand to sample, and the tissue sample falls into the centrifuge tube 10. The scissors are put down, the tail is disinfected, and the sampling is completed. It can be seen that when sampling with the sampling device of this embodiment, there is no need to perform complicated animal restraint actions. Whether it is a skilled sampling technician or a scientific researcher, it can be operated alone to complete safe and efficient mouse tail tissue sampling. This device is simple to operate and can greatly improve the efficiency of single mouse tail tissue sampling, reduce the risk of scientific researchers being bitten, and ensure animal welfare.

[0032] Example 2

[0033] Referring to Figures 2 and 3, a device for sampling tail tissue of an adult mouse comprises a base 1, on which a fixing assembly is fixedly mounted. The fixing assembly is a cover plate 2 having a quarter-conical cylindrical structure, and a cavity is formed between the cover plate 2 and the base 1. The cover plate 2 is open at one end with a larger cross-section, and a slit 3 is provided directly above the cover plate 2, extending from the open end to the other end of the cover plate 2. A storage platform 4 is provided on the side of the base 1 away from the open end of the cover plate 2, and a fixing plate 5 is provided on the storage platform 4. The fixing plate 5 is provided with several holes, and the holes are used to fix a centrifuge tube 10 for collecting tissue samples. Two columns 6 are provided on the base 1, and the two columns 6 are respectively located on both sides of the open end of the cover plate 2. The two columns 6 are provided with a slide groove 7 opposite to each other, and a movable plate 8 is provided between the two columns 6. Slide blocks are provided on both sides of the movable plate 8. The movable plate 8 moves up and down in the slide groove 7 via the slide blocks on both sides. The movable plate 8 is used to seal the open end of the cover plate 2.

[0034] The specific operation of this embodiment is as follows:

[0035] First, when the sampler needs to sample the mouse tail, he or she places a fixed plate 5 on the storage table 4 and a centrifuge tube 10 in the hole on the fixed plate 5. Then, the researcher only needs to gently pull the animal's tail with one hand, drag the mouse into the open end of the cover plate 2, and make the tail pass through the gap. The mouse body enters the cover plate upside down, completely physically isolating the rest of the animal's body from the researcher. The movable plate 8 is lowered to seal the open end of the cover plate 2. At this time, the researcher uses the other hand to cut the tail and drop the tissue sample into the centrifuge tube 10. The scissors are lowered to disinfect the tail, and the sampling is complete.

[0036] The sampling device of this embodiment is used for sampling, which can achieve the effect of embodiment 1. In addition, when the operator accidentally fails to hold the mouse's tail, the movable plate 8 can effectively prevent the mouse from escaping.

[0037] Example 3

[0038] Referring to Figures 2 and 3, a device for sampling tail tissue of an adult mouse comprises a base 1, on which a fixing assembly is fixedly mounted. The fixing assembly is a cover plate 2 having a quarter-conical cylindrical structure, and a cavity is formed between the cover plate 2 and the base 1. The cover plate 2 is open at one end with a larger cross-section, and a slit 3 is provided directly above the cover plate 2, extending from the open end to the other end of the cover plate 2. A storage platform 4 is provided on the side of the base 1 away from the open end of the cover plate 2, and a fixing plate 5 is provided on the storage platform 4. The fixing plate 5 is provided with several holes, and the holes are used to fix a centrifuge tube 10 for collecting tissue samples. Two columns 6 are provided on the base 1, and the two columns 6 are respectively located on both sides of the open end of the cover plate 2. The two columns 6 are provided with a slide groove 7 opposite to each other, and a movable plate 8 is provided between the two columns 6. Slide blocks are provided on both sides of the movable plate 8. The movable plate 8 moves up and down in the slide groove 7 via the slide blocks on both sides. The movable plate 8 is used to seal the open end of the cover plate 2. A connecting plate 9 is installed at the top of the movable plate 8, and a first through hole is opened on both sides of the top of the connecting plate 9, and a locking bolt 11 is installed on the first through hole, and the connecting plate 9 is fixedly connected to the movable plate 8 through the locking bolt 11; a first threaded hole is opened in the middle of the connecting plate 9, and a screw 12 is installed on the first threaded hole, and a hand wheel 13 is fixedly installed on the upper end of the screw 12; a second through hole is provided in the middle of the movable plate 8, and the screw 12 passes through the second through hole, and a second threaded hole 14 is provided on the base 1 corresponding to the second through hole.

[0039] The specific operation of this embodiment is as follows:

[0040] First, when the sampler needs to sample the mouse tail, a fixed plate 5 is placed on the storage table 4, and a centrifuge tube 10 is placed in the hole on the fixed plate 5. Then the researcher only needs to gently pull the animal's tail with one hand, drag the mouse into the open end of the cover plate 2, so that the tail passes through the gap, and the mouse body enters the cover plate upside down, achieving complete physical isolation of other parts of the animal's body from the researcher, put down the movable plate 8 for sealing the open end of the cover plate 2, and use the other hand to turn the screw 12 so that the screw 12 enters the second threaded hole 14 on the base 1, and fix the movable plate 8 on the base 1 to prevent the mouse from escaping. At this time, the tail is cut and the tissue sample falls into the centrifuge tube 10, the scissors are put down, the tail is disinfected, and the sampling is completed. Using the sampling device of this embodiment for sampling, the effect of Example 2 can be achieved, and when the operator accidentally fails to hold the mouse tail tightly, the screw 12 enters the second threaded hole 14 on the base 1, preventing the movable plate 8 from being lifted up by the mouse and escaping.

[0041] As a preferred embodiment of the present invention, the base 1 is a stainless steel plate, and the pillars 2 are made of stainless steel to prevent adult mice from biting or scratching the base. The pillars 2 are welded to the base 1.

[0042] As a preferred embodiment of the present invention, the cover plate 2 is a transparent cover plate made of transparent plastic material, which is convenient for operators to observe the status of adult mice.

[0043] As a preferred embodiment of the present invention, the movable plate 8 is a fan-shaped plate structure, and the connecting plate 9 is a curved plate structure, which fits the fan-shaped outer side surface of the movable plate 8.

[0044] As a preferred embodiment of the present invention, the starting and ending ends of the slits 3 on the cover plate 2 are rounded to prevent sharp edges from scratching mice and ensure animal welfare.

[0045] The above description of the present invention is exemplified in conjunction with the accompanying drawings. It is obvious that the specific implementation of the present invention is not limited to the above-mentioned method. As long as such non-substantial improvements are made using the method concept and technical solution of the present invention, or the concept and technical solution of the present invention are directly applied to other occasions without improvement, they are all within the scope of protection of the present invention.

Claims

1. A device for sampling tail tissue of adult mice, characterized in that: The invention comprises a base (1), on which a fixing component is fixedly mounted, the fixing component being a cover plate (2) having a conical surface structure, a cavity being formed between the cover plate (2) and the base (1), the cover plate (2) having a larger cross-section and an open end, a gap (3) being provided on the cover plate (2), and the gap (3) extending from the open end to the other end of the cover plate (2); A storage platform (4) is provided on a side of the base (1) away from the opening end of the cover plate (2), a fixing plate (5) is provided on the storage platform (4), and a plurality of holes are provided on the fixing plate (5).

2. The adult mouse tail tissue sampling device according to claim 1, characterized in that: The base (1) is provided with two columns (6), the two columns (6) are respectively located on both sides of the opening end of the cover plate (2), the two columns (6) are provided with sliding grooves (7) facing each other, a movable plate (8) is provided between the two columns (6), sliders are provided on both sides of the movable plate (8), the movable plate (8) moves up and down in the sliding groove (7) through the sliders on both sides, and the movable plate (8) is used to seal the opening end of the cover plate (2).

3. The adult mouse tail tissue sampling device according to claim 2, characterized in that: A connecting plate (9) is installed at the top of the movable plate (8), first through holes are provided on both sides of the top of the connecting plate (9), locking bolts (11) are installed on the first through holes, and the connecting plate (9) is fixedly connected to the movable plate (8) via the locking bolts (11); A first threaded hole is provided in the middle of the connecting plate (9), a screw rod (12) is mounted on the first threaded hole, and a hand wheel (13) is fixedly mounted on the upper end of the screw rod (12); a second through hole is provided in the middle of the movable plate (8), the screw rod (12) passes through the second through hole, and a second threaded hole (14) is provided on the base (1) at a position corresponding to the second through hole.

4. The adult mouse tail tissue sampling device according to claim 3, characterized in that: The base (1) is a stainless steel flat plate, and the upright column (6) is made of stainless steel.

5. The adult mouse tail tissue sampling device according to claim 4, characterized in that: The cover plate (2) is a transparent cover plate (2) which is made of a transparent plastic material.

6. The adult mouse tail tissue sampling device according to claim 5, characterized in that: The movable plate (8) is a fan-shaped plate structure.

7. The adult mouse tail tissue sampling device according to claim 6, characterized in that: The connecting plate (9) is a curved plate structure, and two sides thereof are fitted with the fan-shaped outer side surfaces of the movable plate (8).

8. The adult mouse tail tissue sampling device according to claim 7, characterized in that: The starting end and the end of the slit (3) on the cover plate (2) are both rounded.

9. The adult mouse tail tissue sampling device according to claim 8, characterized in that: The upright column (6) is welded to the base (1).

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