Biological tissue attaching sheet and method for manufacturing same

Abstract

A biological tissue attaching sheet 1 includes: an airtight sheet 2 through which a gas and a liquid cannot be permeated; and a plurality of chemical solution-impregnated sheets 3 which are arranged at intervals on one surface of the airtight sheet 2. The airtight sheet 2 includes a base material part 21 and a high heat reflection layer 22 having a higher heat reflectance than that of the base material part 21. The chemical solution-impregnated sheets 3 are impregnated with a chemical solution, and the chemical solution-impregnated sheets 3 are attached to a biological tissue upon use. A method for manufacturing a biological tissue attaching sheet includes: a step (step S11) for applying a glue to a chemical solution-impregnated sheet; a step (step S12) for bonding a release paper liner onto a glue-applied surface of the chemical solution-impregnated sheet; a step (step S14) for transferring the chemical solution-impregnated sheet from the release paper liner to an adhesive sheet; a step (S15) for bonding an airtight sheet to the chemical solution-impregnated sheet; and a step (step S17) for cutting the airtight sheet into a desired shape and peeling off the adhesive sheet.

Description

Biological tissue patch sheet and its manufacturing method

[0001] The present disclosure relates to a biological tissue patch sheet and a method for producing the same.

[0002] Face masks and patches made of sheets impregnated with lotions, toners, creams, and gels have been used in skin care as a means of delivering beauty ingredients and pharmaceuticals to the skin. Typical commercially available face masks have a simple structure, consisting of paper, nonwoven fabric, cotton, etc., punched into a shape that fits the face, and face mask vendors often differentiate their products based on the beauty ingredients and cost. In recent years, multi-layered face masks, which combine aluminum-coated PET film and nonwoven fabric to suppress the evaporation of the toner impregnated into the face mask and provide a wrapping effect, have also been sold.

[0003] In addition, techniques for using weak currents to penetrate active ingredients of cosmetics and pharmaceuticals into the body have attracted attention. Techniques using weak currents are known to be effective in activating cells and enhancing drug penetration, and face masks equipped with standard commercially available batteries as power sources are commercially available. However, power sources using standard commercially available batteries use harmful materials and rare metals, posing challenges in reducing environmental impact and simplifying disposal. To address these challenges, biological tissue patch (Patent Document 1) and sheet mask (Patent Document 2) that do not require a power source and have a low environmental impact are also known.

[0004] Patent No. 6824392 Patent No. 6832435

[0005] Multi-layered face masks, which combine aluminum-deposited PET film and nonwoven fabric, can certainly be expected to have a wrapping effect, but because they are multi-layered, they adhere poorly to the skin compared to nonwoven fabric alone. The time spent wearing a sheet mask for skin care is generally considered a blissful time, a time of relaxation, so poor adhesion when wearing it can be a major psychological issue.

[0006] The present disclosure has been made in view of the above, and aims to provide a biological tissue patch sheet that has the same feel as conventional general biological tissue patch sheets but is expected to be more effective.

[0007] A biological tissue patch sheet according to one embodiment of the present disclosure is a biological tissue patch sheet that is applied to biological tissue and is used by being applied to the biological tissue, and comprises an airtight sheet that is impermeable to gases and liquids, and a plurality of drug solution-impregnated sheets arranged at intervals on one side of the airtight sheet, the airtight sheet comprising a base portion and a high heat reflective layer having a higher thermal reflectivity than the base portion, the drug solution-impregnated sheet being impregnated with a drug solution, and the drug solution-impregnated sheet being applied to the biological tissue for use.

[0008] A manufacturing method of a biological tissue patch sheet according to one embodiment of the present disclosure is a manufacturing method of a biological tissue patch sheet in which a plurality of drug solution-impregnated sheets are attached to an airtight sheet, and includes the steps of: applying glue to the drug solution-impregnated sheet; attaching a release paper liner to the glue-applied surface of the drug solution-impregnated sheet; cutting only the drug solution-impregnated sheet to a desired shape without cutting the release paper liner; attaching an adhesive sheet to the drug solution-impregnated sheet cut to the desired shape, and then peeling off the release paper liner and transferring the drug solution-impregnated sheet from the release paper liner to the adhesive sheet; attaching an airtight sheet to the drug solution-impregnated sheet transferred to the adhesive sheet; cutting the airtight sheet to the desired shape; and peeling off the adhesive sheet after cutting the airtight sheet.

[0009] According to the present disclosure, it is possible to provide a biological tissue patch sheet that has the same feel as a conventional general biological tissue patch sheet, but is expected to be more effective.

[0010] FIG. 1 is an exploded perspective view showing an example of the configuration of a biological tissue patch sheet. FIG. 2 is a view showing an example of the configuration of a biological tissue patch sheet. FIG. 3 is a view showing an example of a method for manufacturing a biological tissue patch sheet. FIG. 4 is an exploded perspective view showing an example of the configuration of a biological tissue patch sheet provided with electrodes. FIG. 5 is a view showing an example of the cross-sectional configuration of a biological tissue patch sheet provided with electrodes. FIG. 6 is a view showing an example of how electrodes are inserted into the biological tissue patch sheet. FIG. 7 is a view showing an example of how electrodes are inserted into the biological tissue patch sheet. FIG. 8 is a view showing an example of measurement results of an example and a comparative example.

[0011] Hereinafter, embodiments of the present disclosure will be described with reference to the drawings.

[0012] [Configuration of Biological Tissue Patch Sheet] An example of the configuration of the biological tissue patch sheet of this embodiment will be described with reference to FIG.

[0013] The biological tissue patch sheet 1 shown in Fig. 1 has an airtight sheet 2 and a drug solution-impregnated sheet 3. A plurality of drug solution-impregnated sheets 3 are arranged at intervals on one surface of the airtight sheet 2 and are bonded together with glue 4. By arranging the drug solution-impregnated sheets 3 at intervals, the flexibility of the biological tissue patch sheet 1 is improved, allowing for excellent adhesion to the biological tissue.

[0014] The airtight sheet 2 is a sheet that is impermeable to gases and liquids, and is composed of a substrate 21 and a high-heat reflective layer 22. For example, the airtight sheet 2 is made by depositing aluminum onto a PET film. The high-heat reflective layer 22 has a higher heat reflectivity than the substrate 21. In FIG. 1 , the substrate 21 is on the top (outside) and the high-heat reflective layer 22 is on the bottom (inside), but the substrate 21 may be on the bottom (inside) and the high-heat reflective layer 22 on the top (outside). The airtight sheet 2 does not have to have the high-heat reflective layer 22.

[0015] Various materials, such as vinyl, polystyrene, acrylic, metal, and carbon, can be used as the material of the substrate 21. More specifically, examples of the material include polyvinyl chloride, polyvinylidene chloride, polyvinyl alcohol, polystyrene, styrene-acrylonitrile copolymer, styrene-butadiene-acrylonitrile copolymer, high-density polyethylene, medium-density polyethylene, low-density polyethylene, ethylene-vinyl acetate copolymer, polypropylene, homopolymer polyacetal, copolymer polyacetal, polymethyl methacrylate, polyester, PPS, polypropylene, cellophane, acetate, polycarbonate, nylon, polyimide, aluminum foil, gold foil, silver foil, copper foil, titanium foil, SUS foil, carbon cloth, carbon sheet, aluminum mesh, gold mesh, silver mesh, copper mesh, titanium mesh, SUS mesh, aluminum wire, gold wire, silver wire, copper wire, titanium wire, and SUS wire, as well as conductive cloth, conductive rubber, and conductive polymers.

[0016] The highly heat-reflective layer 22 can be made of metal or carbon. More specifically, examples include aluminum, iron, titanium, gold, silver, copper, zinc, nickel, chromium, molybdenum, zirconium, niobium, tin, silicon, and carbon. When the highly heat-reflective layer 22 is attached to biological tissue, radiant heat from the body is reflected, causing a temperature rise. This temperature rise can be expected to have effects such as promoting sweating, stimulating blood circulation, and facilitating the penetration of medicinal solutions by opening pores.

[0017] The drug solution-impregnated sheet 3 is a member for retaining the drug solution and coming into contact with biological tissue. The drug solution-impregnated sheet 3 is impregnated with the drug solution when the biological tissue patch sheet 1 is used. The drug solution-impregnated sheet 3 may be made of any material capable of containing the drug solution, such as Japanese paper, cotton, nonwoven fabric, cloth, collagen, polymer gel, bacterial-produced gel, and bacterial-produced xerogel. The bacterial-produced xerogel is porous and therefore has high drug solution retention. Furthermore, the bacterial-produced xerogel gels when it retains the drug solution, providing excellent adhesion to biological tissue.

[0018] The medicinal liquid refers to a medicinal liquid effective against a specific disease, a cosmetic liquid intended to cleanse, beautify, and enhance the attractiveness of the human body, change the appearance, and maintain healthy skin or hair, water, and alcohol. The active ingredient described in Patent Document 2 can be used as the medicinal liquid of this embodiment.

[0019] As the material for the adhesive 4, various materials such as acrylic, urethane, rubber, and silicone can be used. More specifically, acrylic acid ester copolymer, urethane resin, natural rubber, silicone rubber, etc. can be mentioned.

[0020] As shown in Fig. 2, the biological tissue adhesive sheet 1 may be in the shape of a face mask. The biological tissue adhesive sheet 1 shown in the figure comprises an airtight sheet 2 with cutouts at positions corresponding to the eyes and mouth, and a medicinal solution-impregnated sheet 3 arranged at a plurality of positions on the airtight sheet 2. The medicinal solution-impregnated sheet 3 is attached to the airtight sheet 2 with glue 4. The medicinal solution-impregnated sheet 3 is arranged at the positions corresponding to the positions where the medicinal solution is to be infiltrated.

[0021] [Method of Manufacturing Biological Tissue Patch Sheet] An example of a method of manufacturing a biological tissue patch sheet will be described with reference to FIG.

[0022] In the gluing step of step S11, glue 4 is applied to the drug solution impregnated sheet 3. When manufacturing a biological tissue patch sheet having electrodes as described below, it is preferable to apply glue 4 only to a part of the drug solution impregnated sheet 3 rather than to the entire surface of the drug solution impregnated sheet 3.

[0023] In the release paper liner laminating step S12, a release paper liner 6 is laminated to the surface of the chemical solution-impregnated sheet 3 on which the adhesive 4 has been applied. The release paper liner 6 is a material used to protect the adhesive 4. The release paper liner 6 has a smooth surface that minimizes adhesion to the adhesive 4, and can be easily peeled off from the adhesive 4.

[0024] In the half-cutting step of step S13, the chemical solution impregnated sheet 3 to which the release paper liner 6 has been attached is cut into a desired shape. In this step, only the chemical solution impregnated sheet 3 is cut using a punching blade, laser cutter, or the like, without cutting the release paper liner 6. The chemical solution impregnated sheet 3 may be cut in a shape and arrangement to be attached to the airtight sheet 2. For example, the chemical solution impregnated sheet 3 is cut into multiple pieces in the shapes and arrangements shown in Figures 1 and 2. The cut pieces remain attached to the release paper liner 6 in the cut state.

[0025] Unwanted parts (swarf or cuttings) that are produced when the chemical solution-impregnated sheet 3 is cut are peeled off from the release paper liner 6. Although the scraps may be removed manually, using a scrap winder is expected to reduce labor costs and improve production speed.

[0026] In the transfer process of step S14, the chemical solution-impregnated sheet 3 cut to the desired shape in the half-cutting process is attached to the adhesive sheet 7, and then the release paper liner 6 is removed. The release paper liner 6 may be removed manually or by using a winding machine. By making the adhesive strength of the adhesive sheet 7 to the chemical solution-impregnated sheet 3 greater than the adhesive strength of the release paper liner 6 to the glue 4, the release paper liner 6 can be peeled off smoothly.

[0027] In the airtight sheet laminating step S15, the airtight sheet 2 is laminated to the adhesive 4 after the release paper liner 6 has been removed.

[0028] In the half-cutting step of step S16, the airtight sheet 2 attached to the chemical solution-impregnated sheet 3 is cut into a desired shape. In step S16, only the airtight sheet 2 is cut using a punching blade, laser cutter, or the like, without cutting the adhesive sheet 7. For example, the airtight sheet 2 is cut into the shape shown in FIG. 1 or the shape of the face mask shown in FIG. 2. In step S16, the chemical solution-impregnated sheet 3 may be cut simultaneously with the airtight sheet 2. For example, the portion of the chemical solution-impregnated sheet 3 exposed from the outer periphery of the airtight sheet 2 may be cut.

[0029] The unnecessary portion resulting from cutting the airtight sheet 2 is removed from the adhesive sheet 7. The unnecessary portion may be removed manually, or a winding machine may be used to wind up the unnecessary portion of the airtight sheet 2.

[0030] In step S16, the airtight sheet 2 may be cut into a desired shape including the adhesive sheet 7. That is, a biological tissue patch sheet with the adhesive sheet 7 attached thereto is obtained.

[0031] In the adhesive sheet peeling step of step S17, the adhesive sheet 7 is peeled off from the chemical solution impregnated sheet 3. The adhesive sheet 7 may be removed manually or by using a winding machine.

[0032] The adhesive sheet 7 may be a functional adhesive sheet whose adhesive strength decreases when subjected to treatment such as heating, cooling, ultraviolet irradiation, light irradiation, X-ray irradiation, electromagnetic wave irradiation, voltage application, current application, magnetic field application, pressure application, vibration application, or stripping solution penetration, and the adhesive sheet 7 may be peeled off after undergoing a treatment to reduce the adhesive strength. This enables the adhesive sheet 7 to be peeled off smoothly and with minimal damage to the chemical solution-impregnated sheet 3. For example, a thermal release sheet whose adhesive strength decreases when heated is used as the adhesive sheet 7, and in step S17, the adhesive sheet 7 is heated and peeled off from the chemical solution-impregnated sheet 3.

[0033] Through the above steps, the biological tissue patch sheet 1 is obtained.

[0034] In the manufacturing method of the biological tissue patch sheet 1, each step may be performed by a division of labor using separate devices, or may be performed by an assembly line such as a roll-to-roll process.Furthermore, each step may be performed simultaneously.

[0035] [Configuration of Biological Tissue Applying Sheet Having Electrodes] With reference to FIG. 4, an example of the configuration of a biological tissue applying sheet having electrodes will be described.

[0036] The biological tissue patch sheet 1 shown in Fig. 4 has an airtight sheet 2, drug solution-impregnated sheets 3A and 3B, and electrodes 5A and 5B. The electrodes 5A and 5B are disposed between the airtight sheet 2 and the drug solution-impregnated sheets 3A and 3B, and are electrically connected to the airtight sheet 2. The biological tissue patch sheet 1 in Fig. 4 includes the electrodes 5A and 5B, and allows a drug solution to permeate into the inside of the biological tissue by electricity generated by a battery reaction similar to that of a general magnesium-air battery.

[0037] The airtight sheet 2 is composed of a substrate 21 and a high-heat reflective layer 22. The materials for the substrate 21 and the high-heat reflective layer 22 can be the same as those for the biological tissue patch sheet 1 in Figures 1 and 2. In Figure 4, the electrodes 5A and 5B are in contact with the high-heat reflective layer 22, but if the substrate 21 is conductive, the substrate 21 may be in contact with the electrodes 5A and 5B. The rate of the battery reaction can be adjusted by adjusting the electrical resistance of the airtight sheet 2. Increasing the resistance of the airtight sheet 2 slows the rate of iontophoresis of the medicinal solution. If iontophoresis is too rapid and causes pain, the resistance of the airtight sheet 2 can be increased.

[0038] The drug solution-impregnated sheets 3A and 3B are members that are impregnated with drug solution when the biological tissue patch sheet 1 is used. The drug solution-impregnated sheets 3A and 3B may be made of any material that is capable of containing drug solution and is not electrically conductive. The drug solution-impregnated sheets 3A and 3B may be made of the same material as the biological tissue patch sheet 1 shown in FIGS. 1 and 2 .

[0039] The medicinal liquid refers to a medicinal liquid effective against a specific disease, a cosmetic liquid intended to cleanse, beautify, and enhance the attractiveness of the human body, change the appearance, and maintain healthy skin or hair, water, and alcohol. The active ingredient described in Patent Document 2 can be used as the medicinal liquid of this embodiment.

[0040] It is preferable that the drug solution-impregnated sheets 3A and 3B do not come into contact with each other. If the drug solution-impregnated sheets 3A and 3B come into contact with each other, the cell reaction will proceed without the intervention of biological tissue, and the iontophoresis effect of the drug solution will be weakened. Furthermore, by separating the drug solution-impregnated sheets 3A and 3B, the flexibility of the sheets is improved, allowing for excellent adhesion to biological tissue.

[0041] The drug solution-impregnated sheets 3A and 3B are attached to the airtight sheet 2 and electrodes 5A and 5B with glue 4. The same material as that used for the biological tissue patch sheet 1 in Figures 1 and 2 can be used for the glue 4. It is preferable to apply glue 4 only to a portion of the drug solution-impregnated sheets 3A and 3B, rather than to the entire surface of the drug solution-impregnated sheets 3A and 3B. The glue 4 can be applied, for example, in a dotted, striped, grid, or mesh pattern. If the proportion of the surface area of ​​the drug solution-impregnated sheets 3A and 3B to which glue 4 is applied is too high, the glue 4 will inhibit contact between the drug solution and the electrodes 5A and 5B. If the proportion is too low, the adhesive strength will be weak, causing peeling. The area to which glue 4 is applied should be 10-90% of the drug solution-impregnated sheet 3, with 40-60% being optimal.

[0042] [Battery Configuration] The battery unit is composed of an airtight sheet 2, chemical solution-impregnated sheets 3A and 3B, and electrodes 5A and 5B. In the face mask of FIG. 2, electrodes 5A and 5B are disposed on two chemical solution-impregnated sheets 3A and 3B, respectively, to form a battery unit. In other words, the face mask of FIG. 2 includes multiple battery units. One of the electrodes 5A and 5B is a positive electrode, and the other is a negative electrode. In the following description, with reference to FIG. 5, electrode 5A is the negative electrode and electrode 5B is the positive electrode. FIG. 5 shows the components of the battery unit as well as the compounds involved in the reaction. Here, a battery using magnesium is used for the description; however, the present invention is not limited to batteries using magnesium; batteries using aluminum, zinc, or manganese, as well as highly biocompatible biofuel cells, etc., can also be applied.

[0043] When using the biological tissue patch sheet 1, the drug solution-impregnated sheets 3A, 3B are impregnated with a drug solution and brought into contact with the biological tissue (skin) 100, whereby the drug solution acts as an active ingredient and also functions as an electrolyte in the battery section, thereby initiating the battery reaction.

[0044] When water contained in the chemical solution comes into contact with oxygen in the air on the surface of the positive electrode, the reaction shown in the following formula (1) proceeds.

[0045] O2 + 2H2O + 4e - →4OH - (1)

[0046] Meanwhile, the reaction shown in the following formula (2) occurs at the negative electrode in contact with the chemical solution. Specifically, the magnesium constituting the negative electrode releases electrons and dissolves in the chemical solution as magnesium ions. The electrons pass through the airtight sheet 2 and move to the positive electrode.

[0047] Mg → Mg 2+ +2e - (2)

[0048] These reactions occur via the living tissue 100, and the drug solution impregnated in the drug solution-impregnated sheet 3A generates hydroxide ions (OH - ) into the living tissue 100. Furthermore, the movement of hydroxide ions stimulates the muscles, improving muscle sagging, and the movement of hydroxide ions stimulates the lymphatic vessels, improving the flow of lymph in the lymphatic vessels and encouraging the discharge of waste products from the body, which is expected to improve swelling, stiffness, and sagging.

[0049] The overall reaction of the battery is expressed by the following formula (3), which is a reaction that produces magnesium hydroxide. The theoretical electromotive force is approximately 2.7 V.

[0050] 2Mg+O2+2H2O+4e - →2Mg(OH)2 (3)

[0051] The positive electrode can be a positive electrode used in a general magnesium-air battery. For example, carbon, metal, oxide, nitride, carbide, sulfide, and phosphide can be used. Two or more of these may be mixed. The positive electrode may support a catalyst. The catalyst can be a metal, oxide, nitride, carbide, sulfide, and phosphide. Two or more of these may be mixed.

[0052] The positive electrode can be produced by a known process, such as molding carbon powder with a binder. However, fluorine-containing resins are generally used as binders, and when burned for disposal, hydrofluoric acid is generated. By using bacterially produced carbonized cellulose or cellulose nanofiber carbon for the positive electrode, fluorine-containing resins are no longer necessary. As a result, the biological tissue patch sheet 1 of this embodiment reduces environmental impact and can be easily disposed of in daily life. Both bacterially produced carbonized cellulose and cellulose nanofiber carbon are carbonized celluloses with a three-dimensional network structure. These positive electrodes can be produced using the method described in Patent Document 1.

[0053] The negative electrode is composed of a negative electrode active material. The negative electrode active material may be any material that can be used as a negative electrode material for a magnesium-air battery, i.e., any material that includes metallic magnesium or a magnesium-containing material. The negative electrode may be composed of, for example, metallic magnesium, a metallic magnesium sheet, or magnesium powder. Materials other than magnesium that can be used in metal-air batteries, such as iron, zinc, aluminum, calcium, lithium, and sodium, can also be used as a negative electrode material. Magnesium is most suitable from the standpoint of safety and battery output. These negative electrodes can be fabricated using the method described in Patent Document 1.

[0054] [Method of Inserting Electrodes] An example of a method of inserting the electrodes 5A and 5B will be described.

[0055] 3, between the transfer step of step S14 and the airtight sheet lamination step of step S15, an electrode material is placed on the drug solution impregnated sheets 3A and 3B and the glue 4. When the airtight sheet 2 is laminated in step S15, the electrodes 5A and 5B are placed between the airtight sheet 2 and the drug solution impregnated sheets 3A and 3B. This allows the biological tissue patch sheet 1 equipped with electrodes to be produced.

[0056] If it is difficult to insert the electrodes 5A, 5B between the transfer step of step S14 and the airtight sheet laminating step of step S15, the electrodes 5A, 5B are inserted after the adhesive sheet peeling step of step S17. For example, in the half-cutting step of step S16, slits for inserting the electrode material are made in the chemical solution-impregnated sheets 3A, 3B, and after the adhesive sheet peeling step of step S17, the slits are turned up and the electrodes 5A, 5B are inserted.

[0057] Alternatively, instead of slits, spot portions 5C for inserting electrode material are provided in the half-cutting step of step S16, as shown in Fig. 6. After the adhesive sheet peeling step of step S17, electrodes 5A and 5B are placed in each of the spot portions 5C, and the spot portions 5C are sealed with sealing seals 3C, as shown in Fig. 7. The sealing seals 3C can be made from the chemical solution-impregnated sheets 3A and 3B cut when providing the spot portions 5C in the half-cutting step of step S16. While the method of turning up the slits to insert the electrodes 5A and 5B carries the risk of wrinkles occurring when the slits are turned up, the method of placing the electrodes 5A and 5B in the spot portions 5C reduces this risk.

[0058] [Examples] Next, examples in which the materials of the constituent elements of the biological tissue patch sheet were changed and the evaluation results thereof will be described.

[0059] Example 1 Example 1 is a biological tissue patch sheet 1 in the shape of a face mask as shown in FIG. 2, which includes an airtight sheet 2 and 21 drug solution-impregnated sheets 3 .

[0060] The airtight sheet 2 was prepared by cutting a commercially available PET film (thickness: 25 μm) into the shape of a face mask using a punching blade, a laser cutter, or the like.

[0061] The drug solution-impregnated sheet 3 was prepared by cutting commercially available cellulose-based cotton (Bencotton, manufactured by Asahi Kasei) into the desired shape according to the application area using a punching blade, laser cutter, or other suitable tool. An acrylic adhesive (manufactured by Soken Chemical Industries, Ltd.) primarily composed of acrylic ester was applied as glue 4 to the cut drug solution-impregnated sheet 3, dried at 80°C for 1 hour, and then adhered to the airtight sheet 2. A saturated aqueous solution of niacinamide (vitamin B3) was prepared as the drug solution. While niacinamide was used as the drug solution in Example 1, this is not a limitation. In the evaluation test, the biological tissue patch sheet of Example 1 impregnated with the drug solution was applied to subjects, and the skin moisture content was measured 15 minutes later. The biological tissue patch sheet 1 was applied to the faces of 10 subjects ranging in age from their teens to their 60s, and skin care was performed for 15 minutes, followed by skin moisture measurement. The moisture content was measured using a skin moisture meter (manufactured by Scalar Corporation). The results of the evaluation test are described below.

[0062] Example 2 Example 2 differs from Example 1 in that the airtight sheet 2 is provided with an aluminum high heat reflective layer 22. Specifically, VM-PET (25 μm, manufactured by Toray Film Processing Co., Ltd.) in which Al is vapor-deposited to form the high heat reflective layer 22 on a PET film that forms the base material 21 was used as the airtight sheet 2. Other than that, the chemical solution-impregnated sheet 3, chemical solution, and manufacturing method are the same as those in Example 1.

[0063] In the evaluation test, the biological tissue patch sheet 1 of Example 2 impregnated with a medicinal solution was applied to a subject in the same manner as in Example 1, and the moisture content of the skin was measured after 15 minutes. The results of the evaluation test will be described later.

[0064] Example 3 Example 3 differs from Example 2 in that it includes electrodes 5A and 5B. In Example 3, bacterially produced carbonized cellulose, which is carbonized cellulose having a three-dimensional network structure, was used as the positive electrode (electrode 5B), and magnesium was used as the negative electrode (electrode 5A).

[0065] The positive electrode was fabricated as follows. First, we used Nata de Coco (Fujicco), a bacterial cellulose gel produced by the acetic acid bacterium Acetobacter xylinum, as the bacterial gel. The bacterial gel was immersed in liquid nitrogen in a polystyrene foam box for 30 minutes to completely freeze it. After completely freezing, the frozen bacterial gel was placed on a petri dish and dried in a freeze dryer (Tokyo Rikakikai Co., Ltd.) under a vacuum of 10 Pa or less to obtain a bacterial xerogel. After drying in a vacuum, the bacterial xerogel was carbonized by baking at 1200°C for 2 hours in a nitrogen atmosphere to obtain bacterial carbonized cellulose. The resulting bacterial carbonized cellulose was then cut into the desired shape using a punching blade or laser cutter to fabricate the positive electrode.

[0066] The negative electrode was prepared by cutting a commercially available metal magnesium foil (thickness: 200 μm, manufactured by Nilaco) into a desired shape using a punching blade, laser cutter, or the like.

[0067] The positive and negative electrodes were placed so as to be in electrical contact with the conductive surface (aluminum vapor deposition surface) of VM-PET (airtight sheet 2), and sandwiched between VM-PET and chemical solution-impregnated sheets 3A and 3B coated with glue 4. At this time, the positive and negative electrodes were not enclosed within the same chemical solution-impregnated sheets 3A and 3B.

[0068] When applying the glue 4 to the chemical solution-impregnated sheets 3A and 3B, a dot pattern with a diameter of 2 mm was applied using a mold that would regularly arrange dots vertically and horizontally, and would apply the glue 4 to 50% of the area of ​​the chemical solution-impregnated sheets 3A and 3B. As in Example 1, an acrylic adhesive (manufactured by Soken Chemical & Engineering Co., Ltd.) containing acrylic ester as its main component was used for the glue 4. Note that if the glue 4 is applied to the entire surface of the chemical solution-impregnated sheet 3, the glue 4 may inhibit the penetration of the chemicals that initiate the battery reaction.

[0069] In the evaluation test, similar to Example 1, the biological tissue patch sheet 1 of Example 3 impregnated with a medicinal solution was applied to a subject, and the moisture content of the skin was measured after 15 minutes. To initiate the battery reaction, it was confirmed that the medicinal solution had sufficiently permeated the medicinal solution-impregnated sheets 3A and 3B. The results of the evaluation test will be described later. (Comparative Example 1) Comparative Example 1 differs from Example 1 in that the medicinal solution-impregnated sheet 3 is not divided and has the same shape as the airtight sheet 2. The materials, manufacturing method, and evaluation method of the airtight sheet 2 and the medicinal solution-impregnated sheet 3 are the same as those of Example 1. The results of the evaluation test will be described later.

[0070] Comparative Example 2 differs from Example 3 in that the glue 4 is applied to the entire surface instead of being applied in dots. The materials, manufacturing method, and evaluation method of the airtight sheet 2, the chemical solution-impregnated sheets 3A and 3B, and the electrodes 5A and 5B are the same as those of Example 3. The results of the evaluation test will be described later.

[0071] [Evaluation Results] The measurement results for Examples 1-3 and Comparative Examples 1-2 are shown in Figure 8. The figure shows the average values ​​of the moisture content (%) of the skin of 10 subjects measured before use, immediately after use, 1 hour after use, and 3 hours after use for Examples 1-3 and Comparative Examples 1-2.

[0072] It can be seen that in Examples 1-3, the moisture content of the skin increased compared to before use of the sheet mask, more than in Comparative Example 1. This is thought to be because the adhesion between the sheet and the skin was improved by dividing the drug solution-impregnated sheet 3. In contrast, in Comparative Example 1, an increase in moisture content was measured immediately after use, but after 3 weeks, the moisture content had decreased to approximately the same as before use, and no significant change was observed.

[0073] It can be seen that the moisture content is increased in Example 2 compared to Example 1. This is thought to be because the high heat reflective layer 22 reflects radiant heat from the skin, raising the temperature of the area where the sheet mask is used, promoting sweating, promoting blood circulation, and facilitating penetration of the medicinal solution by opening pores.

[0074] It can be seen that the water content is increased in Example 3 compared to Example 2. This is thought to be because ionized niacinamide is introduced into the biological tissue simultaneously with the movement of hydroxide ions into the biological tissue due to the battery reaction.

[0075] In Comparative Example 2, the increase in moisture content was smaller than in Example 3 and was comparable to that in Example 2. This is thought to be because the adhesive was applied to the entire surface, so the chemical solution did not reach the electrodes and the battery reaction did not start, resulting in evaluation results comparable to those of Example 2, which did not have electrodes.

[0076] [Evaluation of Manufacturing Methods] Next, manufacturing methods 1 to 5 and comparative examples and their evaluations will be described.

[0077] (Manufacturing Method 1) In manufacturing method 1, the biological tissue patch sheet was manufactured using a roll-to-roll manufacturing device, rather than by manual labor.

[0078] In the gluing process in step S11, dots with a diameter of 2 mm were arranged regularly in the vertical and horizontal directions, and a mold was used that could be applied to 50% of the chemical-impregnated sheet, and a coater was used to apply dots of glue to the roll-shaped chemical-impregnated sheet.

[0079] In the release paper liner lamination process in step S12, the chemical solution-impregnated sheet coated with adhesive 4 was dried at 100°C while a roll-shaped release paper liner was laminated using a roll-type laminating machine to obtain a chemical solution-impregnated sheet with release paper.

[0080] In the half-cutting step of step S13, a half-cutting device was used to cut only the liquid-impregnated sheet of the release-paper-attached liquid-impregnated sheet into the shape of the liquid-impregnated sheet shown in Figure 2, thereby obtaining a cut adhesive liquid-impregnated sheet. An unnecessary portion of the adhesive liquid-impregnated sheet was peeled off using a waste-collecting winder.

[0081] In the transfer process of step S14, an adhesive sheet was adhered to the cut adhesive-backed chemical-impregnated sheet using a roll-type laminating machine, and the release sheet was peeled off using a winding machine, thereby transferring the chemical-impregnated sheet to the adhesive sheet.

[0082] In the airtight sheet laminating step S15, the chemical solution-impregnated sheet transferred onto the adhesive sheet and the airtight sheet were bonded together using a roll-type laminating machine.

[0083] In the half-cutting step of step S16, the airtight sheet was half-cut into the shape of a face mask using a half-cutting device. Unnecessary airtight sheet waste was removed using a waste-reeling machine.

[0084] In the adhesive sheet peeling step of step S17, the biological tissue patch sheet 1 was obtained by peeling the biological tissue patch sheet from the adhesive sheet.

[0085] With manufacturing method 1, it was possible to produce 600 tissue patch sheets per hour, which was significantly faster than the 20 sheets per hour produced by hand. However, this manufacturing time does not include the drying time of the adhesive.

[0086] (Production Method 2) In production method 2, a biological tissue patch sheet provided with electrodes was produced.

[0087] In manufacturing method 2, after the transfer step of step S14 in manufacturing method 1, the positive electrode and the negative electrode were manually placed on the chemical solution-impregnated sheet, and then the airtight sheet lamination step of step S15 was performed. Otherwise, the manufacturing method was the same as manufacturing method 1.

[0088] With manufacturing method 2, it was possible to produce 100 tissue patch sheets with electrodes per hour, which was significantly faster than the 15 sheets per hour produced by hand. However, this manufacturing time does not include the time for drying the glue or for manufacturing the electrodes.

[0089] (Manufacturing Method 3) Manufacturing Method 3 differs from Manufacturing Method 2 in that after all the steps of Manufacturing Method 1 are completed, electrodes are inserted through the slits.

[0090] In manufacturing method 3, in the half-cutting process of step S13, a half-cutting device was used to cut only the drug-impregnated sheet of the drug-impregnated sheet with release paper into the shape of the drug-impregnated sheet in Figure 2, and incisions were made in the locations where electrodes were to be inserted for insertion.

[0091] After the adhesive sheet peeling step in step S17, the electrodes were inserted through the slits to prepare a biological tissue patch sheet 1 equipped with electrodes.

[0092] Other than the above, the manufacturing method is the same as manufacturing method 2.

[0093] Manufacturing method 3 made it possible to manufacture 300 tissue patch sheets equipped with electrodes per hour, which was significantly higher than the 100 sheets per hour achieved by manufacturing method 2. This was because manufacturing method 2 required only one electrode installer due to work space limitations, as the work was performed using a lawn-to-roll device, whereas manufacturing method 3 allowed five workers to perform the electrode insertion work.

[0094] In manufacturing method 2, it was necessary to turn up the drug solution-impregnated sheet when inserting the electrode, and it was visually observed that slight wrinkles remained on the completed biological tissue patch sheet.

[0095] (Manufacturing Method 4) Manufacturing method 4 differs from manufacturing method 3 in that, instead of making a cut, a spot for placing an electrode is formed, and after the electrode is placed, the electrode is sealed with a sealing seal.

[0096] In manufacturing method 4, in the half-cutting process of step S13, a half-cutting device was used to cut only the drug-impregnated sheet of the drug-impregnated sheet with release paper into the shape of the drug-impregnated sheet in Figure 2, and a spot portion for inserting an electrode was formed in the location where the electrode was to be inserted.

[0097] After the adhesive sheet peeling step in step S17, electrodes were placed on the spots and sealed with a sealing tape to produce a biological tissue patch sheet equipped with electrodes. The sealing tape was produced by reusing unnecessary portions of the adhesive drug solution-impregnated sheet generated during manufacturing.

[0098] Other than the above, the manufacturing method is the same as manufacturing method 3.

[0099] Manufacturing method 4 made it possible to manufacture 400 biological tissue patch sheets equipped with electrodes per hour, which was significantly higher than the 300 sheets per hour produced by manufacturing method 3. This was because manufacturing method 4 eliminated the need for the time-consuming flipping-up process that was required with manufacturing method 3.

[0100] Furthermore, in manufacturing method 4, unlike manufacturing method 3, no wrinkles were observed in the completed biological tissue patch sheet.

[0101] (Production Method 5) Production Method 5 differs from Production Method 4 in that a heat-release sheet is used as the adhesive sheet and further a heat treatment is carried out in the adhesive sheet peeling step.

[0102] In manufacturing method 5, the adhesive sheet used in manufacturing method 4 was changed to a thermal release sheet (manufactured by Nitta), and in the adhesive sheet peeling step of step S17, the biological tissue patch sheet 1 with the thermal release sheet attached was heat-treated in a thermostatic bath at 120°C for 5 minutes to peel off the thermal release sheet. Other than that, this is the same as manufacturing method 4.

[0103] The biological tissue patch sheet produced by production method 5 had a beautiful finish with no fluffing of the drug solution-impregnated sheet. This is because a thermal release sheet was used as the adhesive sheet, and the adhesive strength was reduced by heat treatment, making it possible to easily peel the adhesive sheet from drug solution-impregnated sheets that tend to fluff, such as nonwoven fabric or cotton. Fuzzing of the drug solution-impregnated sheet 3 can also be suppressed when a functional adhesive sheet whose adhesive strength is reduced by treatment such as cooling, ultraviolet light irradiation, light irradiation, X-ray irradiation, electromagnetic wave irradiation, voltage application, current application, magnetic field application, pressure application, vibration application, or penetration of a stripping solution is used as the adhesive sheet.

[0104] (Manufacturing method of the comparative example) In the manufacturing method of the comparative example, the airtight sheet and the chemical solution-impregnated sheet were bonded together with glue without using a release paper liner or an adhesive sheet, and then the chemical solution-impregnated sheet and the airtight sheet were cut into the desired shape.

[0105] First, the liquid-impregnated sheet in the form of a roll was coated with the paste in the form of dots using a coater.

[0106] Thereafter, the adhesive-coated chemical solution-impregnated sheet was dried at 100°C, while a roll-shaped airtight sheet was attached to it using a roll-type laminating machine, to obtain a chemical solution-impregnated sheet with an airtight sheet.

[0107] Thereafter, using a half-cutting device, only the drug solution impregnated sheet of the drug solution impregnated sheet with airtight sheet was cut into the shape of the drug solution impregnated sheet shown in Figure 2. However, it was difficult to half-cut only the drug solution impregnated sheet without cutting the airtight sheet 2, and it was not possible to produce a sheet for application to biological tissue.

[0108] This is because the airtight sheet is very thin, at 25 μm thick, and the adhesive chemical solution impregnated sheet is not flat because it is glued in dots, so when the chemical solution impregnated sheet is punched out, the airtight sheet is also punched out at the same time. In this embodiment, the chemical solution impregnated sheet is laminated to a release paper liner, and then the chemical solution impregnated sheet is punched out. By using a release paper liner that is thick enough to not be cut when the chemical solution impregnated sheet is half-cut, the chemical solution impregnated sheet can be cut into the desired shape.

[0109] As described above, the biological tissue patch sheet 1 of this embodiment includes an airtight sheet 2 that is impermeable to gases and liquids, and a plurality of drug solution-impregnated sheets 3 arranged at intervals on one side of the airtight sheet 2. The airtight sheet 2 includes a substrate 21 and a high-heat-reflecting layer 22 that has a higher heat reflectivity than the substrate 21. The drug solution-impregnated sheet 3 is impregnated with a drug solution and attached to biological tissue for use. The divided drug solution-impregnated sheet 3 improves adhesion. The provision of the high-heat-reflecting layer 22 in the airtight sheet 2 reflects radiant heat from the body when attached to biological tissue, causing a temperature rise. This temperature rise is expected to have effects such as promoting sweating, promoting blood circulation, and facilitating drug solution penetration by opening pores.

[0110] According to this embodiment, the airtight sheet 2 is conductive, and electrodes 5A and 5B are provided between the airtight sheet 2 and the chemical solution-impregnated sheets 3A and 3B, respectively, thereby providing the effect of ion introduction.

[0111] The method for manufacturing a biological tissue patch sheet of this embodiment includes the steps of applying glue to a drug solution impregnated sheet (step S11), laminating a release paper liner to the glue-coated surface of the drug solution impregnated sheet (step S12), cutting only the drug solution impregnated sheet into a desired shape without cutting the release paper liner (step S13), transferring the drug solution impregnated sheet from the release paper liner to an adhesive sheet (step S14), laminating an airtight sheet to the drug solution impregnated sheet (step S15), cutting the airtight sheet into a desired shape (step S16), and peeling off the adhesive sheet (step S17). This allows for the manufacture of a biological tissue patch sheet comprising multiple divided drug solution impregnated sheets, providing a biological tissue patch sheet that is similar in feel to conventional general biological tissue patch sheets but is more effective.

[0112] According to this embodiment, a thermal release sheet whose adhesive strength decreases when heated is used as the adhesive sheet, and in the process of peeling the adhesive sheet, the adhesive sheet is peeled off by heating, thereby preventing fuzzing of the drug solution-impregnated sheet and providing a beautifully finished biological tissue patch sheet.

[0113] REFERENCE SIGNS LIST 1 Biological tissue patch sheet 2 Airtight sheet 21 Base material 22 High heat reflective layer 3, 3A, 3B Drug solution impregnated sheet 3C Sealing seal 4 Glue 5A, 5B Electrode 5C Spot portion 6 Release paper liner 7 Adhesive sheet 100 Biological tissue

Claims

1. A biological tissue patch sheet to be attached to biological tissue, comprising: an airtight sheet which is impermeable to gases and liquids; and a plurality of drug solution-impregnated sheets arranged at intervals on one side of the airtight sheet, the airtight sheet comprising a base material and a high heat reflective layer having a higher thermal reflectivity than the base material, the drug solution-impregnated sheet being impregnated with a drug solution and the drug solution-impregnated sheet being attached to biological tissue for use.

2. A biological tissue patch sheet according to claim 1, wherein the airtight sheet is conductive, and a positive electrode or a negative electrode is provided between the airtight sheet and the plurality of drug solution-impregnated sheets.

3. A method for manufacturing a biological tissue patch sheet having a plurality of drug solution impregnated sheets attached to an airtight sheet, comprising the steps of: applying glue to the drug solution impregnated sheet; laminating a release paper liner to the glue-coated surface of the drug solution impregnated sheet; cutting only the drug solution impregnated sheet into a desired shape without cutting the release paper liner; laminating an adhesive sheet to the drug solution impregnated sheet cut to the desired shape, and then peeling off the release paper liner and transferring the drug solution impregnated sheet from the release paper liner to the adhesive sheet; laminating an airtight sheet to the drug solution impregnated sheet transferred to the adhesive sheet; cutting the airtight sheet into the desired shape; and peeling off the adhesive sheet after cutting the airtight sheet.

4. A method for producing a biological tissue patch sheet according to claim 3, wherein in the step of applying the glue, the glue is applied only to a part of the drug solution-impregnated sheet.

5. A method for manufacturing a biological tissue patch sheet according to claim 3 or 4, comprising the steps of: arranging a positive electrode and a negative electrode on the drug solution impregnated sheet prior to a step of laminating an airtight sheet to the drug solution impregnated sheet transferred to the adhesive sheet.

6. A method for manufacturing a biological tissue patch sheet according to claim 3 or 4, wherein in the step of cutting only the drug solution impregnated sheet into a desired shape, slits or spots for inserting positive and negative electrodes are formed, and after peeling off the adhesive sheet, the positive and negative electrodes are disposed between the airtight sheet and the drug solution impregnated sheet.

7. A method for manufacturing a biological tissue patch sheet according to claim 3 or 4, wherein the adhesive sheet is a functional adhesive sheet whose adhesive strength decreases when a specified treatment is applied, and in the step of peeling off the adhesive sheet, the specified treatment is applied to peel off the adhesive sheet.

8. A method for manufacturing a biological tissue patch sheet according to claim 7, wherein the adhesive sheet is a thermal release sheet whose adhesive strength decreases when heated, and in the step of peeling off the adhesive sheet, the adhesive sheet is peeled off by heating.

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