Cryopreservation formulations

Cryopreservation formulations combining glycerol, trehalose, ascorbic acid, and taurine address the limitations of DMSO-based methods by enhancing cell survival and viability, providing an effective DMSO-free solution for preserving mammalian cells and tissues.

WO2025019671A1PCT designated stage expired Publication Date: 2025-01-23COLOSSAL BIOSCIENCES INC
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Patent Information

Application Number
PCT/US2024/038545
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-07-18
Filing Date
2024-07-18
Publication Date
2025-01-23

AI Technical Summary

Technical Problem

Current cryopreservation methods using dimethyl sulfoxide (DMSO) as a cryoprotectant agent have drawbacks, including potential toxicity to cells, and existing DMSO-free alternatives may not be effective for all applications.

Method used

The development of cryopreservation formulations and media that utilize a combination of cryoprotective agents such as glycerol, trehalose, ascorbic acid, and taurine, without DMSO, to effectively preserve mammalian cells and tissues.

Benefits of technology

These formulations demonstrate improved cell survival and viability post-thawing compared to traditional methods, offering a DMSO-free alternative that is effective for cryopreserving a wide range of mammalian cells and tissues.

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Abstract

The disclosure provides for cryopreservation formulations, cryopreservation media and method of cryopreserving mammalian cells or tissues. The cryopreservation formulations and media contain a combination of cryoprotective agents selected from the group consisting of; (a) glycerol and trehalose; (b) glycerol, trehalose, and ascorbic acid; (c) glycerol, trehalose, and taurine: and (d) glycerol, trehalose, ascorbic acid, and taurine. In certain embodiments, the cryopreservation formulations and media are free of dimethyl sulfoxide.
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Citation Information

Patent Citations

  • Cryopreservation of stem cells

    US20220104481A1

  • Cryopreservation and cell culture media

    US20220159945A1

  • Cryoprotecting agent, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation

    US20230097342A1

  • Composition comprising glass forming agent(s) for use as cryoprotectants and methods of making and using the same

    WO2023064216A1

  • Cryopreservation compositions and methods using red blood cells

    WO2023122672A1

Cited By

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    CN119746089A

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