Gene sequencing kit

Abstract

Disclosed in the present invention is a gene sequencing kit, comprising a housing, a reagent storage box and a pipette tip. The reagent storage box is installed at the bottom of the housing, the housing being rotatable with respect to the reagent storage box; the reagent storage box is provided with at least two reagent storage cavities; the pipette tip is installed in the housing, the pipette tip being rotatable together with the housing with respect to the reagent storage box, and the pipette tip being movable with respect to the housing. The gene sequencing kit solves the problem in the prior art that an outer tub guiding both the rotation and the ascent / descent of an upper cover makes the upper cover prone to deviate from the horizontal direction and then jam.

Description

Gene sequencing kits Technical Field

[0001] The present invention relates to a kit, and in particular to a gene sequencing kit. Background Art

[0002] During the gene sequencing process, reagents are required. In order to realize automatic aspiration of reagents, a sequencing library preparation kit is required. The following functions need to be realized in the kit: storing reagents. During the storage of reagents, each reagent needs to be stored separately to avoid affecting subsequent reagents during each aspiration process. Therefore, it is necessary to aspirate reagents at one position each time to avoid leakage of reagents.

[0003] In the prior art, a sequencing library preparation cartridge comprises: a housing with an opening at the top for a drive mechanism to penetrate; a reagent cartridge installed within the housing and hermetically connected to the housing, the reagent cartridge having multiple storage cavities; a movable assembly equipped with a gun tip and an interface for connecting to an external drive mechanism; the movable assembly rotatably connected within the housing and movable along the axial direction of the housing so that the gun tip is selectively aligned with and inserted into the storage cavity; a hermetically sealed cavity is formed between the movable assembly and the reagent cartridge.

[0004] Although the above-mentioned sequencing library preparation cartridge can realize the movable assembly to sequentially aspirate the reagents in each storage chamber, ensuring that the subsequent storage chambers are in a sealed state when the reagents in the previous storage chamber are aspirated, the sequencing library preparation cartridge still has the following disadvantages: since the movable assembly contains an upper cover and a gun tip, the outer barrel is relatively deep. The outer barrel not only guides the rotation of the upper cover, but also guides the upper cover to rise and fall. The upper cover and the gun tip rise and fall and rotate together. When the external driving mechanism presses down on the upper cover, it is inevitable that the upper cover will be pressed crooked. That is, the top surface of the upper cover is not in the horizontal direction, and the inner hole of the outer barrel is strictly in the vertical direction. The inner wall of the outer barrel will not fit tightly with the side of the upper cover, resulting in the phenomenon that the upper cover is in close contact at some places and a large gap at other places. At this time, the upper cover will be stuck on the outer barrel during lifting or rotation, resulting in the inability to continue the sequential liquid aspiration work. Technical issues

[0005] The outer barrel guides both the rotation and lifting of the upper cover, which may easily cause the upper cover to get stuck after deviating from the horizontal direction. Technical Solutions

[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: The present invention discloses a gene sequencing kit, comprising: a shell, a reagent storage box and a gun tip; a reagent storage box is installed at the bottom of the shell, and the shell can rotate relative to the reagent storage box; the reagent storage box has at least two reagent storage cavities; a gun tip is installed in the shell, and the gun tip can rotate relative to the reagent storage box together with the shell, and the gun tip can move relative to the shell.

[0007] Preferably, a guide hole is provided in the housing to guide the movement of the gun head.

[0008] Preferably, the end face of the shell is in close contact with the end face of the reagent storage box, the shell surface is recessed to form an exhaust hole, the exhaust hole is connected to the guide hole, the reagent storage box has a through hole for the gun head to pass through, the through hole is opposite to the guide hole, and the exhaust hole is offset from the through hole.

[0009] Preferably, the gun tip includes: a first end section, a first transition section and a second end section, the size of the first end section is larger than the size of the second end section, the first end section is located on the side of the second end section away from the reagent storage box, and the first end section is connected to the second end section through the first transition section; the guide hole guides the movement of the first end section in the gun tip.

[0010] Preferably, the shape of the bottom end of the guide hole matches the shape of the first transition section of the gun head, and the exhaust hole is located in the radial direction of the guide hole.

[0011] Preferably, the exhaust hole is a rectangular structure or an elliptical structure located on the edge of the guide hole.

[0012] Preferably, a wall of the shell close to the reagent storage box is a mounting wall, the mounting wall extends toward the reagent base box to form a guide cylinder, the guide cylinder protrudes to form a clamping protrusion, an injection molding through hole is opened on the mounting wall, the injection molding demolding direction of the shell is the X direction, the surface parallel to the X direction is the demolding vertical surface, the projection surface of the injection molding through hole on the demolding vertical surface is the first projection surface, the projection surface of the clamping protrusion on the demolding vertical surface is the second projection surface, and the first projection surface covers the second projection surface.

[0013] Preferably, there are at least two locking protrusions, and the surface of the guide tube facing away from the shell is recessed to form a notch, and the notch is located between two adjacent locking protrusions.

[0014] Preferably, the surface of the locking convex away from the axis of the guide cylinder is an extrusion surface, which can be extruded with the inner wall of the rotating hole opened in the reagent bottom box, and the extrusion surface is a partial conical side structure.

[0015] Preferably, the gun tip comprises: a first end section, a second end section, and a first transition section, the first end section being located above the second end section, the first end section being larger than the second end section, the second end section being a needle-like structure, the second end section being configured to extend into the reagent storage cavity of the external reagent base box, and the top end of the first end section protruding to form a convex ring;

[0016] Preferably, the guide hole includes: a first guide section, a second guide section and an outlet section, the second guide section is located between the first guide section and the outlet section, the first guide section guides the convex ring, the second guide section guides the first end section, the shape of the outlet section matches the first transition section, an elastic member is installed in the first guide section, the first guide section and the second guide section are connected to form a limiting step, and the elastic member is located between the convex ring and the limiting step.

[0017] Preferably, an arc-shaped step is formed at the connection between the second guide section and the outlet section, and the arc-shaped step leads to the first transition section.

[0018] Preferably, the cross-sectional edge of the first transition section along the axis direction is an arc structure.

[0019] Preferably, the gun tip includes: a first end section, a second end section, an intermediate section, a first transition section and a second transition section, the first end section is located above the second end section, the first end section and the intermediate section are hollow cylindrical structures or hollow frustum structures, the second end section is a needle-type structure, the intermediate section is arranged between the first end section and the second end section, the outer diameter of the first end section is greater than the outer diameter of the intermediate section, the outer diameter of the intermediate section is greater than the outer diameter of the second end section, the outer diameter of the intermediate section is greater than the outer diameter of the second end section, the top end of the intermediate section is connected to the first end section through the first transition section, and the bottom end of the intermediate section is connected to the second end section through the second transition section; the plane where the outer wall of the first transition section is located is perpendicular to the axis of the first end section, a right-angle limit step is formed at the first transition section, and a blocking step is provided in the reagent storage box in the genetic testing instrument test kit, and the blocking step limits the right-angle limit step from jumping over.

[0020] Preferably, the inner wall of the first transition section is an arc structure. Beneficial effects

[0021] In this application, the traditional method is changed. Instead of using an outer barrel to guide both the lifting and rotation of the upper cover, the outer barrel is implemented in two parts: one part is that the outer shell can rotate relative to the reagent storage box, and the outer shell and the gun head can rotate together, so that the rotating gun head is aligned with each reagent storage cavity; the other part is that the gun head is lifted relative to the outer shell, and no longer rotates relative to the outer shell. After the above improvement, the external drive mechanism only needs to rotate the outer shell to drive the gun head to rotate relative to the reagent storage box, so that the gun head and the reagent storage cavity are aligned. Subsequently, the external drive mechanism only drives the gun head to lift and lower, that is, lift and lower. The two rotation and lifting movements are independent of each other and do not interfere with each other. This prevents deflection and avoids the occurrence of jamming, ensuring that the liquid aspiration work is carried out in an orderly manner.

[0022] Other advantages, objectives and features of the present invention will be reflected in part from the following description and will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] FIG1 is a schematic structural diagram of the outer shell of the gene sequencing kit in Example 1.

[0024] FIG2 is an exploded view of the outer shell of the gene sequencing kit in Example 1.

[0025] FIG3 is a schematic diagram of the structure of the gene sequencing kit in Example 1.

[0026] FIG4 is a cross-sectional view of the gene sequencing kit in Example 1.

[0027] Figure 5 is a schematic structural diagram of the outer shell of the gene sequencing kit in Example 2.

[0028] FIG6 is an exploded view of the gene sequencing kit in Example 2.

[0029] FIG7 is a cross-sectional view of the housing and the gun head in Example 3.

[0030] FIG8 is an enlarged view of the gun tip in FIG7 .

[0031] FIG9 is a schematic diagram of the structure of the gun tip in the genetic testing instrument kit in Example 4.

[0032] FIG10 is a front view of the gun head in Example 4.

[0033] FIG11 is a cross-sectional view of the gun head in Example 4.

[0034] FIG12 is a cross-sectional view of the reagent storage box and the gun tip in the genetic testing instrument kit in Example 4.

[0035] Figure numerals: outer shell 1, guide hole 11, first guide section 110, outlet section 111, second guide section 112, exhaust hole 12, gun tip 2, first end section 21, convex ring 211, second end section 22, first transition section 23, middle section 24, second transition section 25, reagent storage box 3, blocking step 30, outer shell 1, injection molding through hole 13, guide cylinder 4, notch 40, clamping protrusion 5, extrusion surface 51, elastic member 6. Best Mode for Carrying Out the Invention

[0036] In order to make the technical means, creative features, objectives and functions of the present invention clearer and easier to understand, the present invention is further described below with reference to the accompanying drawings and specific embodiments: Example 1

[0037] As shown in Figures 1 to 4, the present invention discloses a gene sequencing kit, comprising: a shell 11, a reagent storage box 3 and a gun tip 2; the reagent storage box 3 is installed at the bottom of the shell 11, and the shell 11 can rotate relative to the reagent storage box 3; the reagent storage box 3 is provided with at least two reagent storage cavities; the gun tip 2 is installed in the shell 11, and the gun tip 2 can rotate relative to the reagent storage box 3 together with the shell 11, and the gun tip 2 can move relative to the shell 11.

[0038] A guide hole 11 is provided in the housing 11 , and the guide hole 11 guides the movement of the gun head 2 .

[0039] The end face of the shell 11 is in close contact with the end face of the reagent storage box 3. The surface of the shell 11 is recessed to form an exhaust hole 12. The exhaust hole 12 is connected to the guide hole 11. The reagent storage box 3 has a through hole for the gun head 2 to pass through. The through hole is opposite to the guide hole 11, and the exhaust hole 12 is offset from the through hole. Since the present application adds an exhaust hole 12 on the original basis, the end face of the shell 11 is in close contact with the end face of the reagent storage box 3, and the perforation is located at the end face of the reagent storage box 3. After the exhaust hole 12 is added, when the gun tip 2 is inserted into the perforation of the reagent storage box 3, the gas in the guide hole 11 enters the exhaust hole 12. Since the exhaust hole 12 deviates from the perforation, most of the gas rushes to the end face of the reagent storage box 3 and then is rushed to the gap between the end face of the shell 11 and the end face of the reagent storage box 3 and is eliminated. It is difficult to enter the perforation and then affect the amount of liquid aspirated. Therefore, the exhaust hole 12 is set to balance the air pressure in the gap between the end face of the shell 11 and the end face of the reagent storage box 3, avoid entering the reagent storage chamber due to a large airflow flowing to the perforation, and avoid excessive changes in the air pressure in the reagent storage chamber and the amount of liquid aspirated. Because the amount of biological reagent aspirated each time is very small, the change in air pressure directly causes the amount aspirated, thereby improving the measurement accuracy of the amount of liquid aspirated.

[0040] The gun tip 2 includes: a first end section 21, a first transition section 23 and a second end section 22. The size of the first end section 21 is larger than that of the second end section 22. The first end section 21 is located on the side of the second end section 22 away from the reagent storage box 3. The first end section 21 is connected to the second end section 22 through the first transition section 23; the guide hole 11 guides the movement of the first end section 21 in the gun tip 2.

[0041] The shape of the bottom end of the guide hole 11 matches the shape of the first transition section 23 of the gun head 2 , and the exhaust hole 12 is located in the radial direction of the guide hole 11 .

[0042] The exhaust hole 12 is a rectangular or elliptical structure located on the edge of the guide hole 11 . Modes for Carrying Out the Invention

[0043] Example 2

[0044] As shown in Figures 5 and 6, the present invention discloses a gene sequencing kit, including: a shell 11, a reagent storage box 3 and a gun tip 2; the reagent storage box 3 is installed at the bottom of the shell 11, and the shell 11 can rotate relative to the reagent storage box 3; the reagent storage box 3 is provided with at least two reagent storage cavities; the gun tip 2 is installed in the shell 11, and the gun tip 2 can rotate relative to the reagent storage box 3 together with the shell 11, and the gun tip 2 can move relative to the shell 11.

[0045] A guide hole 11 is provided in the housing 11 , and the guide hole 11 guides the movement of the gun head 2 .

[0046] A wall of the shell 11 close to the reagent storage box 3 is a mounting wall, which extends toward the reagent base box to form a guide cylinder 4, which protrudes to form a locking protrusion 5. An injection molding through-hole 13 is provided on the mounting wall. The injection molding demolding direction of the shell 11 is the X direction, and the surface parallel to the X direction is the demolding vertical surface. The projection surface of the injection molding through-hole 13 on the demolding vertical surface is the first projection surface, and the projection surface of the locking protrusion 5 on the demolding vertical surface is the second projection surface. The first projection surface covers the second projection surface. On the basis of the original design of the card convex 5 and the guide cylinder 4, an injection molding through hole 13 is additionally designed. The injection molding through hole 13 is directly opposite to the card convex 5. When the mounting wall is a flat surface and the mounting wall is perpendicular to the demoulding direction, the plane where the mounting wall is located is also a demoulding vertical plane. During demoulding, as long as the first projection surface of the injection molding through hole 13 fully covers the second projection surface, that is, the entire area occupied by the second projection surface is within the area included in the first projection surface, during demoulding, the injection molding through hole 13 can be used for the components on the upper mold in the injection mold to pass through and extend into a position close to the card convex 5. The same shape as the card convex 5 is opened on the lower mold in the injection mold, so that the card convex 5 can be injection molded, and the shell 11 with the guide cylinder 4 and the card convex 5 can be obtained by one-time demoulding, thereby improving production efficiency and saving the cost of the injection mold.

[0047] There are at least two latching protrusions 5. The surface of the guide tube 4 facing away from the housing 11 is recessed to form a notch 40, which is located between two adjacent latching protrusions 5. The notch 40 allows the middle portion of the guide tube 4 to undergo a certain elastic deformation when the latching protrusion 5 is squeezed, thereby squeezing the latching protrusion 5 until it can pass through the rotating hole defined in the reagent base cartridge. After passing through the rotating hole, the latching protrusion 5 hooks onto the reagent base cartridge.

[0048] The surface of the latch 5 facing away from the axis of the guide tube 4 is a pressing surface 51. The pressing surface 51 can press against the inner wall of the rotating hole defined in the reagent base box. The pressing surface 51 is a partially conical side structure. The design of the pressing surface 51 allows the latch 5 to slide smoothly on the inner wall of the rotating hole when pressed. Example 3

[0049] As shown in Figures 7 and 8, the present invention discloses a gene sequencing kit, including: a shell 11, a reagent storage box 3 and a gun tip 2; the reagent storage box 3 is installed at the bottom of the shell 11, and the shell 11 can rotate relative to the reagent storage box 3; the reagent storage box 3 is provided with at least two reagent storage cavities; the gun tip 2 is installed in the shell 11, and the gun tip 2 can rotate relative to the reagent storage box 3 together with the shell 11, and the gun tip 2 can move relative to the shell 11.

[0050] A guide hole 11 is provided in the housing 11 , and the guide hole 11 guides the movement of the gun head 2 .

[0051] The gun tip 2 includes: a first end section 21, a second end section 22, and a first transition section 23. The first end section 21 is located above the second end section 22. The first end section 21 is larger than the second end section 22. The second end section 22 is a needle-like structure and is used to extend into the reagent storage cavity of the external reagent base box. The top end of the first end section 21 protrudes to form a convex ring 211.

[0052] The guide hole 11 includes: a first guide section 110, a second guide section 112 and an outlet section 111. The second guide section 112 is located between the first guide section 110 and the outlet section 111. The first guide section 110 guides the convex ring 211, and the second guide section 112 guides the first end section 21. The shape of the outlet section 111 matches the first transition section 23. An elastic member 6 is installed in the first guide section 110. The first guide section 110 and the second guide section 112 are connected to form a limiting step. The elastic member 6 is located between the convex ring 211 and the limiting step. On the basis of the original product (the original product's shell 11 is provided with a first guide section 110 and an outlet section 111), a second guide section 112 is added and designed. After the gun tip 2 of this product is installed in the shell 11, an elastic member 6 needs to be installed. The gun tip 2 passes through the elastic member 6 so that the gun tip 2 can automatically retract into the shell 11. Therefore, an elastic member 6 must be provided. At the same time, in order to reduce the height of the original product and facilitate the front end of the gun tip 2 to be able to extend into a smaller reagent storage cavity, the gun tip 2 is designed to adopt a multi-section variable diameter design (that is, including a first end section 21, a second end section 22 and a first transition section 23 of different diameters). Since the second end section 22 needs to extend into the external reagent storage cavity, it is certainly impossible to guide the second end section 22 after extending it. This application designs a second guide section 112 to guide the first end section 21 which is located at a higher position and has a higher height, so that when the gun tip 2 is inserted into a precise position at the bottom of the reagent storage chamber, the second end section 22 of the gun tip will be aligned with a precise position at the bottom of the reagent storage chamber to avoid the phenomenon of deflection, thereby ensuring the accuracy of the insertion position of the second end section 22, because the shape design of the bottom wall of the reagent storage chamber was also mentioned in the previous patent application to guide the liquid and magnetic beads to a precise position. This is also to cooperate with the hardcover position when the liquid can be fully sucked away, and at the same time, the second end section 22 will not deviate to the magnetic bead adsorption position on one side due to poor guidance. The precise coordination between them is very necessary in gene sequencing experiments and is conducive to the separation of magnetic beads and aspirated liquid.

[0053] The connection between the second guide section 112 and the outlet section 111 forms an arc-shaped step, which leads to the first transition section 23. This ensures that the gun head 2 can be supported by the arc-shaped step when it is pressed down by an external driving device.

[0054] The cross-sectional edge of the first transition section 23 along the axis is an arc structure, which enables the second transition section 25 to slide smoothly on the first transition section 23. At the same time, when the first transition section 23 fully overlaps with the inner wall of the outlet section 111, it can fully support the gun head 2 and prevent the gun head 2 from moving further downward. Example 4

[0055] As shown in Figures 9 to 12, the present invention discloses a gene sequencing kit, including: a shell 11, a reagent storage box 3 and a gun tip 2; the reagent storage box 3 is installed at the bottom of the shell 11, and the shell 11 can rotate relative to the reagent storage box 3; the reagent storage box 3 is provided with at least two reagent storage cavities; the gun tip 2 is installed in the shell 11, and the gun tip 2 can rotate relative to the reagent storage box 3 together with the shell 11, and the gun tip 2 can move relative to the shell 11.

[0056] A guide hole 11 is provided in the housing 11 , and the guide hole 11 guides the movement of the gun head 2 .

[0057] The gun head 2 includes: a first end section 21, a second end section 22, an intermediate section 24, a first transition section 23 and a second transition section 25. The first end section 21 is located above the second end section 22. The first end section 21 and the intermediate section 24 are hollow cylindrical structures or hollow frustum structures. The second end section 22 is a needle-type structure. The intermediate section 24 is arranged between the first end section 21 and the second end section 22. The outer diameter of the first end section 21 is larger than the outer diameter of the intermediate section 24, and the outer diameter of the intermediate section 24 is larger than the outer diameter of the second end section 22. The outer diameter of the middle section 24 is larger than the outer diameter of the second end section 22. The top of the middle section 24 is connected to the first end section 21 through the first transition section 23, and the bottom of the middle section 24 is connected to the second end section 22 through the second transition section 25. The plane where the outer wall of the first transition section 23 is located is perpendicular to the axis of the first end section 21. A right-angle limit step is formed at the first transition section 23. A blocking step 30 is provided in the reagent storage box 3 in the genetic testing instrument kit to prevent the right-angle limit step from jumping over. By improving the structure of the first transition section 23, the original outer wall of the first transition section 23, which was originally a hollow frustum structure, is changed into a right-angled surface perpendicular to the axis of the first end section 21. This avoids the problem that the first transition section 23 has a certain elasticity when pressed down due to the outer wall of the first transition section 23 being a hollow frustum structure in the prior art. In the present application, the plane where the blocking step 30 is located is also parallel to the plane where the outer wall of the first transition section 23 is located. The first transition section 23 will not jump over the blocking step 30 due to the elasticity of the first transition section 23, thereby protecting the entire genetic testing ritual gun tip 2 and avoiding the entire liquid aspiration work being affected by the genetic testing ritual gun tip 2 being compressed and deformed, thereby ensuring that the entire biochemical reaction can proceed smoothly.

[0058] The inner wall of the first transition section 23 is an arc structure to avoid right angles on the inner wall of the first transition section 23 that would cause liquid to settle there, thereby avoiding contamination of the first transition section 23 and affecting the amount of liquid added in each step, thus ensuring that the entire biochemical reaction can proceed normally.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not limiting. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A gene sequencing kit, characterized in that: include: Housing, reagent storage box and gun tip; A reagent storage box is installed at the bottom of the shell, and the shell can rotate relative to the reagent storage box; The reagent storage box is provided with at least two reagent storage chambers; A gun head is installed in the shell. The gun head can rotate relative to the reagent storage box together with the shell, and the gun head can move relative to the shell.

2. The gene sequencing kit according to claim 1, characterized in that: A guide hole is arranged in the shell, and the guide hole guides the movement of the gun head.

3. The gene sequencing kit according to claim 2, characterized in that: The end face of the shell is in close contact with the end face of the reagent storage box, the shell surface is concave to form an exhaust hole, the exhaust hole is connected to the guide hole, the reagent storage box is provided with a through hole for the gun head to pass through, the through hole is opposite to the guide hole, and the exhaust hole is offset from the through hole.

4. The gene sequencing kit according to claim 3, characterized in that: The gun head includes: A first end section, a first transition section and a second end section, the first end section is larger than the second end section, the first end section is located on the side of the second end section away from the reagent storage box, and the first end section is connected to the second end section through the first transition section; the guide hole guides the movement of the first end section in the gun head.

5. The gene sequencing kit according to claim 4, characterized in that: The shape of the bottom end of the guide hole matches the shape of the first transition section of the gun head, and the exhaust hole is located in the radial direction of the guide hole.

6. The gene sequencing kit according to claim 5, characterized in that: The exhaust hole is a rectangular structure or an elliptical structure located on the edge of the guide hole.

7. The gene sequencing kit according to claim 1, characterized in that: A wall of the shell close to the reagent storage box is a mounting wall, which extends toward the reagent bottom box to form a guide cylinder, which protrudes to form a clamping protrusion, and an injection molding through hole is opened on the mounting wall. The injection molding demolding direction of the shell is the X direction, and the surface parallel to the X direction is the demolding vertical surface. The projection surface of the injection molding through hole on the demolding vertical surface is the first projection surface, and the projection surface of the clamping protrusion on the demolding vertical surface is the second projection surface, and the first projection surface covers the second projection surface.

8. The gene sequencing kit according to claim 7, characterized in that: There are at least two clamping protrusions, and the surface of the guide tube facing away from the shell is recessed to form a notch, and the notch is located between two adjacent clamping protrusions.

9. The gene sequencing kit according to claim 8, characterized in that: The surface of the clamping convex away from the axis of the guide tube is an extrusion surface, which can be extruded with the inner wall of the rotating hole opened in the reagent bottom box, and the extrusion surface is a partial cone side structure.

10. The gene sequencing kit according to claim 2, characterized in that: The gun head includes: A first end section, a second end section and a first transition section, wherein the first end section is located above the second end section, the size of the first end section is larger than the size of the second end section, the second end section is a needle-type structure, the second end section is used to extend into the reagent storage cavity of the external reagent bottom box, and the top end of the first end section protrudes to form a convex ring; The guide hole includes: a first guide section, a second guide section and an outlet section, the second guide section is located between the first guide section and the outlet section, the first guide section guides the convex ring, the second guide section guides the first end section, the shape of the outlet section matches the first transition section, an elastic member is installed in the first guide section, the first guide section and the second guide section are connected to form a limiting step, and the elastic member is located between the convex ring and the limiting step.

11. The gene sequencing kit according to claim 10, characterized in that: An arc-shaped step is formed at the connection between the second guide section and the outlet section, and the arc-shaped step leads to the first transition section.

12. The gene sequencing kit according to claim 11, characterized in that: The cross-sectional edge of the first transition section along the axis direction is an arc structure.

13. The gene sequencing kit according to claim 2, characterized in that: The gun head includes: A first end section, a second end section, an intermediate section, a first transition section and a second transition section, wherein the first end section is located above the second end section, the first end section and the intermediate section are hollow cylindrical structures or hollow frustum structures, the second end section is a needle-type structure, an intermediate section is arranged between the first end section and the second end section, the outer diameter of the first end section is greater than the outer diameter of the intermediate section, the outer diameter of the intermediate section is greater than the outer diameter of the second end section, the outer diameter of the intermediate section is greater than the outer diameter of the second end section, the top end of the intermediate section is connected to the first end section through the first transition section, and the bottom end of the intermediate section is connected to the second end section through the second transition section; the plane where the outer wall of the first transition section is located is perpendicular to the axis of the first end section, a right-angle limit step is formed at the first transition section, a blocking step is arranged in the reagent storage box in the genetic testing instrument test kit, and the blocking step limits the right-angle limit step from jumping over.

14. The gene sequencing kit according to claim 13, characterized in that: The inner wall of the first transition section is an arc structure.

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