Pesticidal genes and methods of use

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

Description

[0001]Atty Docket No. G1224241100WO (0143.1)   PESTICIDAL GENES AND METHODS OF USE   CROSS REFERENCE TO RELATED APPLICATIONS This application claims the benefit of priority to U.S. Provisional Application No. 63 / 610,901, filed on December 15, 2023, the entire contents of which are incorporated by reference herein. STATEMENT REGARDING THE SEQUENCE LISTING The official copy of the sequence listing is submitted electronically concurrently with the specification as an XML formatted sequence listing with a file name of AgB137WO_SeqList.xml, created on December 13, 2024, and having a size of 30,851 bytes. The sequence listing contained in this XML formatted document is part of the specification and is herein incorporated by reference in its entirety. FIELD OF THE INVENTION The invention is drawn to methods and compositions for controlling pests, particularly plant pests. BACKGROUND Pests, plant diseases, and weeds can be serious threats to crops. Losses due to pests and diseases have been estimated at 37% of the agricultural production worldwide, with 13% due to insects, bacteria and other organisms. Toxins are virulence determinants that play an important role in microbial pathogenicity and / or evasion of the host immune response. Toxins from the gram- positive bacterium Bacillus, particularly Bacillus thuringiensis, have been used as insecticidal proteins. Current strategies use the genes expressing these toxins to produce transgenic crops. Transgenic crops expressing insecticidal protein toxins are used to combat crop damage from insects. While the use of Bacillus toxins has been successful in controlling insects, resistance to Bt toxins has developed in some target pests in many parts of the world where such toxins have been used intensively. One way of solving this problem is sowing Page 1 of 97 WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) Bt crops with alternating rows of regular non Bt crops (refuge). An alternative method to avoid or slow down development of insect resistance is stacking insecticidal genes with different modes of action against insects in transgenic plants. The current strategy of using transgenic crops expressing insecticidal protein toxins is placing increasing emphasis on the discovery of novel toxins, beyond those already derived from the bacterium Bacillus thuringiensis. Pleurotolysin is a pore-forming cytolysin produced by the oyster mushroom, Pleurotus ostreatus. Pleurotolysin comprises a 17kDa component, pleurotolysin A (plyA), and a 59 kDa component, pleurotolysin B (plyB), which assemble to form a transmembrane pore complex and causes hemolysis of cells. These toxins may prove useful as alternatives to those derived from B. thuringiensis for deployment in insect- and pest-resistant transgenic plants. Thus, new toxin proteins are needed. SUMMARY Compositions having pesticidal activity and methods for their use are provided. Compositions include polypeptide sequences including isolated and recombinant polypeptide sequences having pesticidal activity, nucleic acid molecules including isolated, recombinant, and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the DNA constructs or vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest, including microorganisms and plants. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. These organisms and compositions comprising the organisms are desirable for agricultural purposes. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Page 2 of 97     WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included. BRIEF DESCRIPTION OF THE DRAWINGS FIG.1 illustrates a sequence alignment of plyA amino acid sequences of ATG12694.0 (APG58918.0 / SEQ ID NO:2) and ATG12694.4 (APG58918.4 / SEQ ID NO: 10). FIG.2 illustrates a sequence alignment of plyB amino acid sequences of ATG12692.1 (APG57949.1 / SEQ ID NO: 14) and ATG12692.3 (APG57949.3 / SEQ ID NO: 18). FIG.3 illustrates a sequence alignment of the plyA sequences APG58918.0, APG58918.1, APG58918.2, APG58918.3, and APG58918.4. FIG.4 illustrates a sequence alignment of the plyB sequences APG57949.0, APG57949.1, APG57949.2, and APG57949.3. FIG.5 depicts the results a diet overlay bioassay for susceptible WCRW. pGA9885 is the identifier for the combination of APG57949.1 and APG58918.4 at a 1:3 ratio (binary toxin). The percent mortality, percent molting, and weight (mg) of the larvae are provided. FIG.6 depicts the results a diet overlay bioassay for Cry34 / 35-R (resistant) WCRW. pGA9885 is the identifier for the combination of APG57949.1 and APG58918.4 at a 1:3 ratio (binary toxin). The percent mortality, percent molting, and weight (mg) of the larvae are provided. FIG.7 depicts the results of a transgenic maize root excision assay. Maize were transformed with APG57949.1 (AFG75914.1) / APG54101.0 (AFG54101.0) in combination or individually. Sample roots from transformed corn plants were infested with WCRW larvae and scored on day 4 on a 9 point scale. Page 3 of 97     WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) DETAILED DESCRIPTION OF THE INVENTION The present inventions now will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the inventions are shown. Indeed, these inventions may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements. Like numbers refer to like elements throughout. Many modifications and other embodiments of the inventions set forth herein will come to mind to one skilled in the art to which these inventions pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the inventions are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation. I. Polynucleotides and Polypeptides Compositions and method for conferring pesticidal activity to an organism are provided. The modified organism exhibits pesticidal resistance or tolerance. Recombinant pesticidal proteins, or polypeptides and fragments and variants thereof that retain pesticidal activity, are provided and include those set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18. The pesticidal proteins are biologically active (e.g., pesticidal) against pests including insects, fungi, nematodes, and the like. Nucleotide sequences encoding the pesticidal polypeptides are provided and include those set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, and 17. Nucleotide sequences encoding the pesticidal polypeptides, including for example, SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, or active fragments or variants thereof, can be used to produce transgenic organisms, such as plants and microorganisms. The pesticidal proteins are biologically active (for example, are pesticidal) against pests including insects, fungi, nematodes, and the like. In specific embodiments, the pesticidal polypeptides and the active variant and fragments Page 4 of 97     WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) thereof have an improved pesticidal activity when compared to other polypeptides in the art. Polynucleotides encoding the pesticidal polypeptides, including for example, SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, or active fragments or variants thereof, can be used to produce transgenic organisms, such as plants and microorganisms.  The transformed organisms are characterized by genomes that comprise at least one stably incorporated DNA construct comprising a coding sequence for a pesticidal protein disclosed herein. In some embodiments, the coding sequence is operably linked to a promoter that drives expression of the encoded pesticidal polypeptide. Accordingly, transformed microorganisms, plant cells, plant tissues, plants, seeds, and plant parts are provided. The pesticidal polypeptides of SEQ ID NO: 2, 4, 6, 8, and 10 are characterized as plyA sequences and the pesticidal polypeptides of SEQ ID NO: 12, 14, 16, and 18 are characterized as plyB sequences. In some embodiments, the plyA and plyB sequences are used in combination. Any combination of the plyA and plyB sequences provided herein can be used in the present invention. In other embodiments, the plyA and plyB sequences are used separately. A summary of various polypeptides, active variants and fragments thereof, and polynucleotides encoding the same are set forth below in Table 1. As noted in Table 1, various forms of polypeptides are provided. Full length pesticidal polypeptides, as well as modified versions of the original full-length sequence (i.e., variants) are provided. Page 5 of 97     WBD (US) 4854-4443-2791v1 ytir%alim tfm or,dsea8,3,8, , ,sof8, 9, 98,838,99senadit eeste78297 7,2,7di(topn eht ohesy, , 9, 8, 9, 9,eopilytc gthuni etti 681969681969dgra,lnoend5, , 0 , , , 0vyl ocul nivliwol 80,95,90,15,8050,9,9,1,Pni o cpneni am his eb4898499948984999e g,h )0, , , , , , ,teniw 8682999086829997fmvolo se,9,5,1,7,9,5,1, 9s den eaads aheecb 78 9 9 7 8979 f(it en d ,oth soeet8,74,80,96,98,74,80,9696di opn ethuqsil,7,3, , , , , ,egeopilc gt euni es yt7, 89,85,97,073,89,85,9, 0aP ytlnoenvydd locul%itn672,884062840,8,9,1,78891Pni opnc eeenihtdi571878399,95,71,87,83,999en sesalAyB lyG C PlP d,eif 951idD:)oI s,7 ,3q( )OT,5 1,MeS NN(,31171hqe:-l tgSO lunFeTNL NDI 1de0ifi:dD)1, ,oI sq( )8,61MeOA S NA 6(,,441 81 h:1qv-l tlgeSO1unAN972- FeL ADI 221 3444-e 0. 4m8a1 )0.9)58N9 se 49 se4)e8c cn5n 7GAeu5nS U GBeu (ePyl qyeP l qD G AP( sAP(esB W Atty Docket No. G1224241100WO (0143.1) i. Classes of Pesticidal proteins The pesticidal proteins provided herein and the nucleotide sequences encoding them are useful in methods for impacting pests. That is, the compositions and methods of the invention find use in agriculture for controlling or killing pests, including pests of many crop plants. The pesticidal proteins provided herein are toxin proteins from mushrooms and / or bacteria and exhibit activity against certain pests. The pesticidal proteins are from several classes of toxins including pleurotolysin (ply), Cry, Cyt, BIN, and Mtx toxins. See, for example, Table 1 for the specific protein classifications of the various SEQ ID NOs provided herein. In addition, reference is made throughout this disclosure to Pfam database entries. The Pfam database is a database of protein families, each represented by multiple sequence alignments and a profile hidden Markov model. Finn et al. (2014) Nucl. Acid Res. Database Issue 42:D222-D230. Pleurotolysin (ply) is a pore-forming cytolysin that forms a transmembrane pore complex and belongs to the MACPF (membrane-attack complex / perforin) superfamily. Ply is a fungal toxin (e.g., produced by the mushroom genus Pleurotus), can bind to lipids in membranes comprising sphingomyelin, cholesterol, and / or ceramide phosphoethanolamine, and was identified in the edible mushroom Pleurotus ostreatus (Tomita, T, et al., 2004, J. Biol. Chem.279(26):26975-82, herein incorporated by reference in its entirety). The ply cytolysin is made up of two components, a 17 kDa plyA, and a 59 kDa plyB. PlyA is an aegerolysin-like protein and plyB has an MACPF domain. The lipid-binding mechanism by which ply binds to membranes is different from other similar toxins, such as Cry toxins or bacterial aegerolysin-based toxins, which associate with protein receptors. Bacillus thuringiensis (Bt) is a gram-positive bacterium that produces insecticidal proteins as crystal inclusions during its sporulation phase of growth. The proteinaceous inclusions of Bacillus thuringiensis (Bt) are called crystal proteins or δ-endotoxins (or Cry proteins), which are toxic to members of the class Insecta and other invertebrates. Similarly, Cyt proteins are parasporal inclusion proteins from Bt that exhibit hemolytic (cytolytic) activity or have obvious sequence similarity to a known Cyt protein. These toxins are highly specific to their target organism, but are innocuous to humans, vertebrates, and plants. Page 7 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) The structure of the Cry toxins reveals five conserved amino acid blocks, concentrated mainly in the center of the domain or at the junction between the domains. The Cry toxin consists of three domains, each with a specific function. Domain I is a seven α-helix bundle in which a central helix is completely surrounded by six outer helices. This domain is implicated in channel formation in the membrane. Domain II appears as a triangular column of three anti-parallel β–sheets, which are similar to antigen–binding regions of immunoglobulins. Domain III contains anti-parallel β–strands in a β sandwich form. The N-terminal part of the toxin protein is responsible for its toxicity and specificity and contains five conserved regions. The C-terminal part is usually highly conserved and probably responsible for crystal formation. See, for example, U.S. Patent No.8,878,007. Strains of B. thuringiensis show a wide range of specificity against different insect orders (Lepidoptera, Diptera, Coleoptera, Hymenoptera, Homoptera, Phthiraptera or Mallophaga, and Acari) and other invertebrates (Nemathelminthes, Platyhelminthes, and Sarocomastebrates). The Cry proteins have been classified into groups based on toxicity to various insect and invertebrate groups. Generally, Cry I demonstrates toxicity to lepidopterans, Cry II to lepidopterans and dipterans, CryIII to coleopterans, Cry IV to dipterans, and Cry V and Cry VI to nematodes. New Cry proteins can be identified and assigned to a Cry group based on amino acid identity. See, for example, Bravo, A. (1997) J. of Bacteriol.179:2793-2801; Bravo et al. (2013) Microb. Biotechnol.6:17-26, herein incorporated by reference. Over 750 different cry gene sequences have been classified into 73 groups (Cry1– Cry73), with new members of this gene family continuing to be discovered (Crickmore et al. (2014) www.btnomenclature.info / ). The cry gene family consists of several phylogentically non-related protein families that may have different modes of action: the family of three-domain Cry toxins, the family of mosquitocidal Cry toxins, the family of the binary-like toxins, and the Cyt family of toxins (Bravo et al., 2005). Some Bt strains produce additional insecticidal toxins, the VIP toxins. See, also, Cohen et al. (2011) J. Mol. Biol.413:4-814; Crickmore et al. (2020) Journal of Invertebrate Pathology, doi:10.1016 / j.jip.2020.107438; Crickmore et al. (2014) Bacillus thuringiensis toxin nomenclature, found on the world wide web at Page 8 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) lifesci.sussex.ac.uk / home / Neil_Crickmore / Bt / ; Crickmore et al. (1988) Microbiol. Mol. Biol. Rev.62: 807-813; Gill et al. (1992) Ann. Rev. Entomol.37: 807-636; Goldbert et al. (1997) Appl. Environ. Microbiol.63:2716-2712; Knowles et al. (1992) Proc. R. Soc. Ser. B.248: 1-7; Koni et al. (1994) Microbiology 140: 1869-1880; Lailak et al. (2013) Biochem. Biophys. Res. Commun.435: 216-221; Lopez-Diaz et al. (2013) Environ. Microbiol.15: 3030-3039; Perez et al. (2007) Cell. Microbiol.9: 2931-2937; Promdonkoy et al. (2003) Biochem. J.374: 255-259; Rigden (2009) FEBS Lett.583: 1555-1560; Schnepf et al. (1998) Microbiol. Mol. Biol. Rev.62: 775-806; Soberon et al. (2013) Peptides 41: 87-93; Thiery et al. (1998) J. Am. Mosq. Control Assoc.14: 472-476; Thomas et al. (1983) FEBS Lett.154: 362-368; Wirth et al. (1997) Proc. Natl. Acad. Sci. U.S.A.94: 10536-10540; Wirth et al (2005) Appl. Environ. Microbiol.71: 185-189; and Zhang et al. (2006) Biosci. Biotechnol. Biochem.70: 2199-2204; each of which is herein incorporated by reference in their entirety. Cyt designates a parasporal crystal inclusion protein from Bacillus thuringiensis with cytolytic activity, or a protein with sequence similarity to a known Cyt protein. (Crickmore et al. (1998) Microbiol. Mol. Biol. Rev.62: 807-813). The gene is denoted by cyt. These proteins are different in structure and activity from Cry proteins (Gill et al. (1992) Annu. Rev. Entomol.37: 615-636). The Cyt toxins were first discovered in B. thuringiensis subspecies israelensis (Goldberg et al. (1977) Mosq. News.37: 355-358). There are 3 Cyt toxin families including 11 holotype toxins in the current nomenclature (Crickmore et al. (2014) Bacillus thuringiensis toxin nomenclature found on the world wide web at lifesci.sussex.ac.uk / home / Neil_Crickmore / Bt / ). The majority of the B. thuringiensis isolates with cyt genes show activity against dipteran insects (particularly mosquitoes and black flies), but there are also cyt genes that have been described in B. thuringiensis strains targeting lepidopteran or coleopteran insects (Guerchicoff et al. (1997) Appl. Environ. Microbiol.63: 2716-2721). The structure of Cyt2A, solved by X-ray crystallography, shows a single domain where two outer layers of α-helix wrap around a mixed β-sheet. Further available crystal structures of Cyt toxins support a conserved α-β structural model with two α-helix hairpins flanking a β-sheet core containing seven to eight β-strands. (Cohen et al. (2011) J. Mol. Biol.413: 804-814) Mutagenic studies identified β-sheet residues as critical for Page 9 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) toxicity, while mutations in the helical domains did not affect toxicity (Adang et al.; Diversity of Bacillus thuringiensis Crystal Toxins and Mechanism of Action. In: T. S. Dhadialla and S. S. Gill, eds, Advances in Insect Physiology, Vol.47, Oxford: Academic Press, 2014, pp.39-87.) The representative domain of the Cyt toxin is a ™-endotoxin, Bac_thur_toxin (Pfam PF01338). There are multiple proposed models for the mode of action of Cyt toxins, and it is still an area of active investigation. Some Cyt proteins (Cyt1A) have been shown to require the presence of accessory proteins for crystallization. Cyt1A and Cyt2A protoxins are processed by digestive proteases at the same sites in the N- and C-termini to a stable toxin core. Cyt toxins then interact with non-saturated membrane lipids, such as phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin. For Cyt toxins, pore-formation and detergent-like membrane disruption have been proposed as non- exclusive mechanisms; and it is generally accepted that both may occur depending on toxin concentration, with lower concentrations favoring oligomeric pores and higher concentrations leading to membrane breaks. (Butko (2003) Appl. Environ. Microbiol.69: 2415-2422) In the pore-formation model, the Cyt toxin binds to the cell membrane, inducing the formation of cation-selective channels in the membrane vesicles leading to colloid-osmotic lysis of the cell. (Knowles et al. (1989) FEBS Lett.244: 259-262; Knowles et al. (1992) Proc. R. Soc. Ser. B.248: 1-7 and Promdonkoy et al. (2003) Biochem. J.374: 255-259). In the detergent model, there is a nonspecific aggregation of the toxin on the surface of the lipid bilayer leading to membrane disassembly and cell death. (Butko (2003) supra; Manceva et al. (2005) Biochem.44: 589-597). Multiple studies have shown synergistic activity between Cyt toxins and other B. thuringiensis toxins, particularly the Cry, Bin, and Mtx toxins. This synergism has even been shown to overcome an insect’s resistance to the other toxin. (Wirth 1997, Wirth 2005, Thiery 1998, Zhang 2006) The Cyt synergistic effect for Cry toxins is proposed to involve Cyt1A binding to domain II of Cry toxins in solution or on the membrane plane to promote formation of a Cry toxin pre-pore oligomer. Formation of this oligomer is independent of the Cyt oligomerization, binding or insertion. (Lailak 2013, Perez 2007, Lopez-Diaz 2013). Page 10 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) A number of pesticidal proteins unrelated to the Cry proteins are produced by some strains of B. thuringiensis and B. cereus during vegetative growth (Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389–5394; Warren et al. (1994) WO 94 / 21795). These vegetative insecticidal proteins, or Vips, do not form parasporal crystal proteins and are apparently secreted from the cell. The Vips are presently excluded from the Cry protein nomenclature because they are not crystal-forming proteins. The term VIP is a misnomer in the sense that some B. thuringiensis Cry proteins are also produced during vegetative growth as well as during the stationary and sporulation phases, most notably Cry3Aa. The location of the Vip genes in the B. thuringiensis genome has been reported to reside on large plasmids that also encode cry genes (Mesrati et al. (2005) FEMS Microbiol. Lett.244(2):353-8). A website for the nomenclature of Bt toxins can be found on the world wide web at lifesci.sussex.ac.uk with the path “ / home / Neil_Crickmore / Bt / ” and at: “btnomenclature.info / ”. See also, Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806. Such references are herein incorporated by reference. Vip genes can be classified into 4 categories. Some Vip genes form binary two- component protein complexes; an "A" component is usually the "active" portion, and a "B" component is usually the "binding" portion. (Pfam pfam.xfam.org / family / PF03495). The Vip1 and Vip4 proteins generally contain binary toxin B protein domains. Vip2 proteins generally contain binary toxin A protein domains. The Vip1 and Vip2 proteins are the two components of a binary toxin that exhibits toxicity to coleopterans. Vip1Aa1 and Vip2Aa1 are very active against corn rootworms, particularly Diabrotica virgifera and Diabrotica longicornis (Han et al. (1999) Nat. Struct. Biol.6:932–936; Warren GW (1997) “Vegetative insecticidal proteins: novel proteins for control of corn pests” In: Carozzi NB, Koziel M (eds) Advances in insect control, the role of transgenic plants; Taylor & Francis Ltd, London, pp 109–21). The membrane-binding 95 kDa Vip1 multimer provides a pathway for the 52 kDa vip2 ADP-ribosylase to enter the cytoplasm of target western corn rootworm cells (Warren (1997) supra). The NAD-dependent ADP-ribosyltransferase Vip2 likely modifies monomeric actin at Arg177 to block polymerization, leading to loss of the actin cytoskeleton and eventual cell death due to the rapid subunit exchange within actin filaments in vivo (Carlier M. F. (1990) Adv. Biophys.26:51–73). Page 11 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Like Cry toxins, activated Vip3A toxins are pore-forming proteins capable of making stable ion channels in the membrane (Lee et al. (2003) Appl. Environ. Microbiol. 69:4648–4657). Vip3 proteins are active against several major lepidopteran pests (Rang et al. (2005) Appl. Environ. Microbiol.71(10):6276-6281; Bhalla et al. (2005) FEMS Microbiol. Lett.243:467–472; Estruch et al. (1998) WO 9844137; Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389–5394; Selvapandiyan et al. (2001) Appl. Environ Microbiol.67:5855–5858; Yu et al. (1997) Appl. Environ Microbiol.63:532–536). Vip3A is active against Agrotis ipsilon, Spodoptera frugiperda, Spodoptera exigua, Heliothis virescens, and Helicoverpa zea (Warren et al. (1996) WO 96 / 10083; Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389–5394). Like Cry toxins, Vip3A proteins must be activated by proteases prior to recognition at the surface of the midgut epithelium of specific membrane proteins different from those recognized by Cry toxins. The MTX family of toxin proteins is characterized by the presence of a conserved domain, ETX_MTX2 (pfam 03318). Members of this family share sequence homology with the mosquitocidal toxins Mtx2 and Mtx3 from Bacillus sphaericus, as well as with the epsilon toxin ETX from Clostridium perfringens (Cole et al. (2004) Nat. Struct. Mol. Biol.11: 797-8; Thanabalu et al. (1996) Gene 170:85-9). The MTX-like proteins are structurally distinct from the three-domain Cry toxins, as they have an elongated and predominately β-sheet-based structure. However, similar to the three-domain toxins, the MTX-like proteins are thought to form pores in the membranes of target cells (Adang et al. (2014) supra). Unlike the three-domain Cry proteins, the MTX-like proteins are much smaller in length, ranging from 267 amino acids (Cry23) to 340 amino acids (Cry15A). The classification of the Mtx-like proteins has been revised to be in the Mpp (Mtx2-like pesticidal proteins) class of beta pore-forming pesticidal proteins from the ETX / MTX2 family. See, Crickmore, et al., 2020, J. Invert. Path., Jul 9:107438, doi: 10.1016 / j.jip.2020.107438, PMID: 32652083. The protein family of MTX-like toxins is a relatively small class compared to the three-domain Cry family (Crickmore et al. (2014) supra; Adang et al. (2014) supra). The members of the MTX-like toxin family include Cry15, Cry23, Cry33, Cry38, Cry45, Cry46, Cry51, Cry60A, Cry60B, and Cry64. This family exhibits a range of insecticidal activity, including activity against insect pests of the Lepidopteran and Coleopteran Page 12 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) orders. Some members of this family may form binary partnerships with other proteins, which may or may not be required for insecticidal activity. Cry15 is a 34 kDA protein that was identified in Bacillus thuringiensis serovar thompsoni HD542; it occurs naturally in a crystal together with an unrelated protein of approximately 40 kDa. The gene encoding Cry15 and its partner protein are arranged together in an operon. Cry15 alone has been shown to have activity against lepidopteran insect pests including Manduca sexta, Cydia pomonella, and Pieris rapae, with the presence of the 40 kDA protein having been shown to increase activity of Cry15 only against C. pomonella (Brown K. and Whiteley H. (1992) J. Bacteriol.174:549-557; Naimov et al. (2008) Appl. Environ. Microbiol.74:7145–7151). Further studies are needed to elucidate the function of the partner protein of Cry15. Similarly, Cry23 is a 29 kDA protein that has been shown to have activity against the coleopteran pests Tribolium castaneum and Popillia japonica together with its partner protein Cry37 (Donovan et al. (2000) US Patent No.6,063,756). New members of the MTX-like family are continuing to be identified. An ETX_MTX toxin gene was recently identified in the genome of Bacillus thuringiensis serovar tolworthi strain Na205-3. This strain was found to be toxic against the lepidpoteran pest Helicoverpa armigera, and it also contained homologs of Cry1, Cry11, Vip1, Vip2, and Vip3 (Palma et al. (2014) Genome Announc.2(2): e00187-14. Published online Mar 13, 2014 at doi: 10.1128 / genomeA.00187-14; PMCID: PMC3953196). Because the MTX-like proteins have a unique domain structure relative to the three- domain Cry proteins, they are believed to possess a unique mode of action, thereby making them a valuable tool in insect control and the fight against insect resistance. Bacterial cells produce large numbers of toxins with diverse specificity against host and non-host organisms. Large families of binary toxins have been identified in numerous bacterial families, including toxins that have activity against insect pests. (Poopathi and Abidha (2010) J. Physiol. Path.1(3): 22-38). Lysinibacillus sphaericus (Ls), formerly Bacillus sphaericus, (Ahmed et al. (2007) Int. J. Syst. Evol. Microbiol. 57:1117-1125) is well-known as an insect biocontrol strain. Ls produces several insecticidal proteins, including the highly potent binary complex BinA / BinB. This binary complex forms a parasporal crystal in Ls cells and has strong and specific activity against Page 13 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) dipteran insects, specifically mosquitos. In some areas, insect resistance to existing Ls mosquitocidal strains has been reported. The discovery of new binary toxins with different target specificity or the ability to overcome insect resistance is of significant interest. The Ls binary insecticidal protein complex contains two major polypeptides, a 42 kDa polypeptide and a 51 kDa polypeptide, designated BinA and BinB, respectively (Ahmed et al. (2007) supra). The two polypeptides act synergistically to confer toxicity to their targets. Mode of action involves binding of the proteins to receptors in the larval midgut. In some cases, the proteins are modified by protease digestion in the larval gut to produce activated forms. The BinB component is thought to be involved in binding, while the BinA component confers toxicity (Nielsen-LeRoux et al. (2001) Appl. Environ. Microbiol.67(11):5049–5054). When cloned and expressed separately, the BinA component is toxic to mosquito larvae, while the BinB component is not. However, co- administration of the proteins markedly increases toxicity (Nielsen-LeRoux et al. (2001) supra). A small number of Bin protein homologs have been described from bacterial sources. Priest et al. (1997) Appl. Environ. Microbiol.63(4):1195-1198 describe a hybridization effort to identify new Ls strains, although most of the genes they identified encoded proteins identical to the known BinA / BinB proteins. The BinA protein contains a defined conserved domain known as the Toxin 10 superfamily domain. This toxin domain was originally defined by its presence in BinA and BinB. The two proteins both have the domain, although the sequence similarity between BinA and BinB is limited in this region (<40%). The Cry49Aa protein, which also has insecticidal activity, also has this domain (described below). The Cry48Aa / Cry49Aa binary toxin of Ls has the ability to kill Culex quinquefasciatus mosquito larvae. These proteins are in a protein structural class that has some similarity to the Cry protein complex of Bacillus thuringiensis (Bt), a well-known insecticidal protein family. The Cry34 / Cry35 binary toxin of Bt is also known to kill insects, including Western corn rootworm, a significant pest of corn. Cry34, of which several variants have been identified, is a small (14 kDa) polypeptide, while Cry35 (also encoded by several variants) is a 44 kDa polypeptide. These proteins have some Page 14 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) sequence homology with the BinA / BinB protein group and are thought to be evolutionarily related (Ellis et al. (2002) Appl. Environ. Microbiol.68(3):1137-1145). The classification of Cry34 has been revised to be in the Gpp class of aegerolysin like pesticidal proteins, such as Gpp34Aa. See, Crickmore, et al., 2020, J. Invert. Path., Jul 9:107438, doi: 10.1016 / j.jip.2020.107438, PMID: 32652083. Phosphoinositide phospholipase C proteins (PI-PLC; also phosphotidylinositol phospholipase C) are members of the broader group of phospholipase C proteins. Many of these proteins play important roles in signal transduction as part of normal cell physiology. Several important bacterial toxins also contain domains with similarity to these proteins (Titball, R.W. (1993) Microbiological Reviews.57(2):347-366). Importantly, these proteins are implicated in signal amplification during intoxication of insect cells by Bt Cry proteins (Valaitis, A.P. (2008) Insect Biochemistry and Molecular Biology.38: 611-618). The PI-PLC toxin class occurs in Bacillus isolates, commonly seen in co- occurrence with homologs to other described toxin classes, such as Binary Toxins. This class of sequences has homology to phosphatidylinositol phosphodiesterases (also referred to as phosphatidylinositol-specific phospholipase C – PI-PLC). The crystal structure and its active site were solved for B. cereus PI-PLC by Heinz et al (Heinz, et. al., (1995) The EMBO Journal.14(16): 3855-3863). The roles of the B. cereus PI-PLC active site amino acid residues in catalysis and substrate binding were investigated by Gässler et al using site-directed mutagenesis, kinetics, and crystal structure analysis (Gässler, et. al., (1997) Biochemistry.36(42):12802-13). These PI-PLC toxin proteins contain a PLC-like phosphodiesterase, TIM beta / alpha-barrel domain (IPR017946) and / or a Phospholipase C, phosphatidylinositol- specific, X domain (IPR000909) (also referred to as the PI-PLC X-box domain). We have also seen proteins with these domains in combination with other typical Bacillus protein toxin domains. This list includes most commonly a lectin domain (IPR000772), a sugar- binding domain that can be present in one or more copies and is thought to bind cell membranes, as well as the Insecticidal crystal toxin (IPR008872) (also referred to as Toxin10 or P42), which is the defining domain of the Binary Toxin. Page 15 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Previously, toxins of this PI-PLC class were defined in U.S. Patent No.8,318,900 B2 SEQ ID NOs: 30 (DNA) and 79 (amino acid), in U.S. Patent Publication No. 20110263488A1 SEQ ID NOs: 8 (DNA) and 9 (amino acid), and in U.S. Patent No. 8,461,421B2 SEQ ID NOs: 3 (DNA) and 63 (amino acid). The Xpp class of pesticidal proteins is a holding class for pesticidal proteins with currently uncharacterized structures. See, e.g., Crickmore et al. (2020) Journal of Invertebrate Pathology, doi:10.1016 / j.jip.2020.107438, which is incorporated by reference herein in its entirety. Provided herein are pesticidal proteins from these classes of toxins. The pesticidal proteins are classified by their structure, homology to known toxins and / or their pesticidal specificity. ii. Variants and Fragments of Pesticidal Proteins and Polynucleotides Encoding the Same Pesticidal proteins or polypeptides of the invention include those set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18, and fragments and variants thereof. By "pesticidal toxin" or "pesticidal protein" or “pesticidal polypeptide” is intended a toxin or protein or polypeptide that has activity against one or more pests, including, insects, fungi, nematodes, and the like such that the pest is killed or controlled. The term "isolated" or "purified" encompasses a polypeptide or protein, or biologically active portion thereof, polynucleotide or nucleic acid molecule, or other entity or substance, that is substantially or essentially free from components that normally accompany or interact with the polypeptide or polynucleotide as found in its naturally occurring environment. Isolated polypeptides or polynucleotides may be separated from at least about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or more of the other components with which they were initially associated. Thus, an isolated or purified polypeptide or protein is substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized. A protein that is substantially free of cellular material includes preparations of protein having less than about 30%, 20%, 10%, 5%, or 1% (by dry Page 16 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) weight) of contaminating protein. When the protein of the invention or biologically active portion thereof is recombinantly produced, optimally culture medium represents less than about 30%, 20%, 10%, 5%, or 1% (by dry weight) of chemical precursors or non-protein-of-interest chemicals. The term “fragment” refers to a portion of a polypeptide sequence of the invention. "Fragments" or "biologically active portions" include polypeptides comprising a sufficient number of contiguous amino acid residues to retain the biological activity, i.e., have pesticidal activity. Fragments of the pesticidal proteins include those that are shorter than the full-length sequences, either due to the use of an alternate downstream start site, or due to processing that produces a shorter protein having pesticidal activity. Processing may occur in the organism the protein is expressed in, or in the pest after ingestion of the protein. Examples of fragments of the proteins can be found in Table 1. A biologically active portion of a pesticidal protein can be a polypeptide that is, for example, 10, 20, 25, 30, 50, 40, 50, 60, 70, 75, 80, 90, 100, 110, 120, 125, 130, 140, 150, 160, 170, 175, 180, 190, 200, 210, 220, 225, 230, 240, 250, 260 or more contiguous amino acids in length of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. Such biologically active portions can be prepared by recombinant techniques and evaluated for pesticidal activity. As used here, a fragment comprises at least 8 contiguous amino acids of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. Bacterial genes, including those encoding the pesticidal proteins disclosed herein, quite often possess multiple methionine initiation codons in proximity to the start of the open reading frame. Often, translation initiation at one or more of these start codons will lead to generation of a functional protein. These start codons can include ATG codons. However, bacteria such as Bacillus sp. also recognize the codon GTG as a start codon, and proteins that initiate translation at GTG codons contain a methionine at the first amino acid. On rare occasions, translation in bacterial systems can initiate at a TTG codon, though in this event the TTG encodes a methionine. Furthermore, it is not often determined a priori which of these codons are used naturally in the bacterium. Thus, it is understood that use of one of the alternate methionine codons may also lead to generation of pesticidal proteins. These pesticidal proteins are encompassed in the present invention and may be used in the methods disclosed herein. It will be understood that, when Page 17 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) expressed in plants, it will be necessary to alter the alternate start codon to ATG for proper translation. In various embodiments the pesticidal proteins provided herein include amino acid sequences deduced from the full-length nucleotide sequences and amino acid sequences that are shorter than the full-length sequences due to the use of an alternate downstream start site. Thus, the nucleotide sequence of the invention and / or vectors, host cells, and plants comprising the nucleotide sequence of the invention (and methods of making and using the nucleotide sequence of the invention) may comprise a nucleotide sequence encoding an alternate start site. It is recognized that modifications may be made to the pesticidal polypeptides provided herein creating variant proteins. Changes designed by man may be introduced through the application of site-directed mutagenesis techniques. Alternatively, native, as yet unknown or as yet unidentified polynucleotides and / or polypeptides structurally and / or functionally related to the sequences disclosed herein may also be identified that fall within the scope of the present invention. Conservative amino acid substitutions may be made in nonconserved regions that do not alter the function of the pesticidal proteins. Alternatively, modifications may be made that improve the activity of the toxin. Modification of Cry toxins by domain III swapping has resulted in some cases in hybrid toxins with improved toxicities against certain insect species. Thus, domain III swapping could be an effective strategy to improve toxicity of Cry toxins or to create novel hybrid toxins with toxicity against pests that show no susceptibility to the parental Cry toxins. Site-directed mutagenesis of domain II loop sequences may result in new toxins with increased insecticidal activity. Domain II loop regions are key binding regions of initial Cry toxins that are suitable targets for the mutagenesis and selection of Cry toxins with improved insecticidal properties. Domain I of the Cry toxin may be modified to introduce protease cleavage sites to improve activity against certain pests. Strategies for shuffling the three different domains among large numbers of cry genes and high through output bioassay screening methods may provide novel Cry toxins with improved or novel toxicities. As indicated, fragments and variants of the polypeptides disclosed herein will retain pesticidal activity. Pesticidal activity comprises the ability of the composition to Page 18 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) achieve an observable effect diminishing the occurrence or an activity of the target pest, including for example, bringing about death of at least one pest, or a noticeable reduction in pest growth, feeding, or normal physiological development. Such decreases in numbers, pest growth, feeding or normal development can comprise any statistically significant decrease, including, for example a decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. The pesticidal activity against one or more of the various pests provided herein, including, for example, pesticidal activity against Coleoptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Nematodes, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, etc., or any other pest described herein.  It is recognized that the pesticidal activity may be different or improved relative to the activity of the native protein, or it may be unchanged, so long as pesticidal activity is retained. Methods for measuring pesticidal activity are provided elsewhere herein. See also,Czapla and Lang (1990) J. Econ. Entomol.83:2480-2485; Andrews et al. (1988) Biochem. J.252:199-206; Marrone et al. (1985) J. of Economic Entomology 78:290-293; and U.S. Pat. No.5,743,477, all of which are herein incorporated by reference in their entirety.   By "variants" is intended polypeptides having an amino acid sequence that is at least about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99% identical to the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, and retain pesticidal activity. Note, Table 1 provides non-limiting examples of variant polypeptides (and polynucleotide encoding the same) for SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. A biologically active variant of a pesticidal polypeptide of the invention may differ by as few as about 1-15 amino acid residues, as few as about 1-10, such as about 6-10, as few as 5, as few as 4, as few as 3, as few as 2, or as few as 1 amino acid residue. In specific embodiments, the polypeptides can comprise an N-terminal or a C-terminal truncation, which can comprise at least a deletion of 10, 15, 20, 25, 30, 35, 40, 45, 50 amino acids or more from either the N or C terminal of the polypeptide. Page 19 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Table 2 provides protein domains found in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18 based on PFAM data. Both the domain description and the positions within a given SEQ ID NO are provided in Table 2. In specific embodiments, the active variant comprising SEQ ID NO: 2 can comprise at least 70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 2 and further comprises at least one of the conserved domains set forth in Table 2. For example, in one embodiment, the active variant will comprise at least 70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:2, and further comprises the native amino acids at positions 1-138. Table 2. Summary of PFAM domains in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, and 18 APG ID Seq Modification PFAM Domain Description Domain ID Type domain Positions Page 20 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) APG57949.3 18 Amino acid 165 294 PF01823 MAC / Perfoin PF18684 Pleurotol sin B C-term 329 499 molecules, encoding the pesticidal polypeptides disclosed herein are also provided and include the sequences set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, and 17. Of particular interest are nucleic acid sequences that have been designed for expression in a plant or a microbe of interest. That is, the nucleic acid sequence can be optimized for increased expression in a host plant or in a host microbe of interest. A pesticidal protein of the invention can be back-translated to produce a nucleic acid comprising codons optimized for expression in a particular host, for example, a crop plant. In another embodiment, the polynucleotides encoding the polypeptides provided herein may be optimized for increased expression in the transformed plant. That is, the polynucleotides can be synthesized using plant-preferred codons for improved expression. See, for example, Campbell and Gowri (1990) Plant Physiol.92:1-11 for a discussion of host- preferred codon usage. Methods are available in the art for synthesizing plant-preferred genes. See, for example, U.S. Patent Nos.5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic Acids Res.17:477-498, herein incorporated by reference. Expression of such a coding sequence by the transformed plant (e.g., dicot or monocot) will result in the production of a pesticidal polypeptide and confer increased resistance in the plant to a pest. Recombinant and synthetic nucleic acid molecules encoding the pesticidal proteins of the invention do not include the naturally occurring bacterial sequence encoding the protein. A “recombinant polynucleotide” or “recombinant nucleic acid” or “recombinant nucleic acid molecule” comprises a combination of two or more chemically linked nucleic acid segments which are not found directly joined in nature. By “directly joined” is intended the two nucleic acid segments are immediately adjacent and joined to one another by a chemical linkage. In specific embodiments, the recombinant polynucleotide comprises a polynucleotide of interest or a variant or fragment thereof such that an additional chemically linked nucleic acid segment is located either 5', 3' or internal to the Page 21 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) polynucleotide of interest. Alternatively, the chemically-linked nucleic acid segment of the recombinant polynucleotide can be formed by deletion of a sequence. The additional chemically linked nucleic acid segment or the sequence deleted to join the linked nucleic acid segments can be of any length, including for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20 or greater nucleotides. Various methods for making such recombinant polynucleotides include chemical synthesis or by the manipulation of isolated segments of polynucleotides by genetic engineering techniques. In specific embodiments, the recombinant polynucleotide can comprise a recombinant DNA sequence or a recombinant RNA sequence. A “fragment of a recombinant polynucleotide or nucleic acid” comprises at least one of a combination of two or more chemically linked amino acid segments which are not found directly joined in nature. A “recombinant polypeptide” or “recombinant protein” is a polypeptide or protein encoded by a recombinant polynucleotide. Fragments of a polynucleotide (RNA or DNA) may encode protein fragments that retain activity. In specific embodiments, a fragment of a recombinant polynucleotide or a recombinant polynucleotide construct comprises at least one junction of the two or more chemically linked or operably linked nucleic acid segments which are not found directly joined in nature. A fragment of a polynucleotide that encodes a biologically active portion of a polypeptide that retains pesticidal activity will encode at least 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, 110, 120, 125, 130, 140, 150, 160, 170, 175, 180, contiguous amino acids, or up to the total number of amino acids present in a full-length polypeptide as set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. In some embodiments, a fragment of a polynucleotide comprises at least 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, 110, 120, 125, 130, 140, 150, 160, 170, 175, 180, 190, 200, 210, 220, 225, 230, 240, 250, 260, 270, 275, 280, 290, 300, 310, 320, 325, 330, 340, 350, 360, 370, 375, 380, 390, 400, contiguous nucleotides, or up the total number of nucleotides present in a full-length nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, or 17. In specific embodiments, such polypeptide fragments are active fragment, and in still other embodiments, the polypeptide fragment comprises a recombinant polypeptide fragment. As used herein, a fragment of a recombinant polypeptide comprises at least one of a Page 22 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) combination of two or more chemically linked amino acid segments which are not found directly joined in nature. By "variants" is intended to mean substantially similar sequences. For polynucleotides, a variant comprises a deletion and / or addition of one or more nucleotides at one or more internal sites within the native polynucleotide and / or a substitution of one or more nucleotides at one or more sites in the native polynucleotide. As used herein, a "native" polynucleotide or polypeptide comprises a naturally occurring nucleotide sequence or amino acid sequence, respectively. Variants of a particular polynucleotide of the invention, including the polynucleotides set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, and 17 (i.e., the reference polynucleotide) can also be evaluated by comparison of the percent sequence identity between the polypeptide encoded by a variant polynucleotide and the polypeptide encoded by the reference polynucleotide. Thus, for example, an isolated polynucleotide that encodes a polypeptide with a given percent sequence identity to the polypeptide of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18 are disclosed. Percent sequence identity between any two polypeptides can be calculated using sequence alignment programs and parameters described elsewhere herein. Where any given pair of polynucleotides of the invention is evaluated by comparison of the percent sequence identity shared by the two polypeptides they encode, the percent sequence identity between the two encoded polypeptides is at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. In other embodiments, the variant of the polynucleotide provided herein differs from the native sequence by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more nucleotides. Variant polynucleotides and proteins also encompass sequences and proteins derived from a mutagenic and recombinogenic procedure such as DNA shuffling. With such a procedure, one or more different pesticidal protein disclosed herein (SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18) is manipulated to create a new pesticidal protein possessing the desired properties. In this manner, libraries of recombinant polynucleotides are generated from a population of related sequence polynucleotides comprising sequence regions that have substantial sequence identity and can be Page 23 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) homologously recombined in vitro or in vivo. For example, using this approach, sequence motifs encoding a domain of interest may be shuffled between the pesticidal sequences provided herein and other known pesticidal genes to obtain a new gene coding for a protein with an improved property of interest, such as an increased Km in the case of an enzyme. Strategies for such DNA shuffling are known in the art. See, for example, Stemmer (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751; Stemmer (1994) Nature 370:389-391; Crameri et al. (1997) Nature Biotech.15:436-438; Moore et al. (1997) J. Mol. Biol.272:336-347; Zhang et al. (1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri et al. (1998) Nature 391:288-291; and U.S. Patent Nos.5,605,793 and 5,837,458. A "shuffled" nucleic acid is a nucleic acid produced by a shuffling procedure such as any shuffling procedure set forth herein. Shuffled nucleic acids are produced by recombining (physically or virtually) two or more nucleic acids (or character strings), for example in an artificial, and optionally recursive, fashion. Generally, one or more screening steps are used in shuffling processes to identify nucleic acids of interest; this screening step can be performed before or after any recombination step. In some (but not all) shuffling embodiments, it is desirable to perform multiple rounds of recombination prior to selection to increase the diversity of the pool to be screened. The overall process of recombination and selection are optionally repeated recursively. Depending on context, shuffling can refer to an overall process of recombination and selection, or, alternately, can simply refer to the recombinational portions of the overall process. In one embodiment, a method of obtaining a polynucleotide that encodes an improved polypeptide comprising pesticidal activity is provided, wherein the improved polypeptide has at least one improved property over SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. Such methods can comprise (a) recombining a plurality of parental polynucleotides to produce a library of recombinant polynucleotides encoding recombinant pesticidal polypeptides; (b) screening the library to identify a recombinant polynucleotide that encodes an improved recombinant pesticidal polypeptide that has an enhanced property improved over the parental polynucleotide; (c) recovering the recombinant polynucleotide that encodes the improved recombinant pesticidal polypeptide identified in (b); and, (d) repeating steps (a), (b) and (c) using the Page 24 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) recombinant polynucleotide recovered in step (c) as one of the plurality of parental polynucleotides in repeated step (a). iii. Sequence Comparisons As used herein, the term "identity" or "percent identity" when used with respect to a particular pair of aligned amino acid sequences or aligned nucleotide sequences, refers to the percent amino acid sequence identity or percent nucleotide sequence identity that is obtained by counting the number of identical matches in the alignment and dividing such number of identical matches by the length of the aligned sequences. As used herein, the term "similarity" or "percent similarity" when used with respect to a particular pair of aligned amino acid sequences or aligned nucleotide sequences, refers to the sum of the scores that are obtained from a scoring matrix for each amino acid pair or each nucleotide pair in the alignment divided by the length of the aligned sequences. Unless otherwise stated, identity and similarity will be calculated by the Needleman-Wunsch global alignment and scoring algorithms (Needleman and Wunsch (1970) J. Mol. Biol.48(3):443-453) as implemented by the “needle” program, distributed as part of the EMBOSS software package (Rice,P. Longden,I. and Bleasby,A., EMBOSS: The European Molecular Biology Open Software Suite, 2000, Trends in Genetics 16, (6) pp276—277, versions 6.3.1 available from EMBnet at embnet.org / resource / emboss and emboss.sourceforge.net, among other sources) using default gap penalties and scoring matrices (EBLOSUM62 for protein and EDNAFULL for DNA). Equivalent programs may also be used. By "equivalent program" is intended any sequence comparison program that, for any two sequences in question, generates an alignment having identical nucleotide residue matches and an identical percent sequence identity when compared to the corresponding alignment generated by needle from EMBOSS version 6.3.1. Additional mathematical algorithms are known in the art and can be utilized for the comparison of two sequences. See, for example, the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2264, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877. Such an algorithm is incorporated into the BLAST programs of Altschul et al. (1990) J. Mol. Biol.215:403. BLAST nucleotide searches can be performed with the BLASTN program (nucleotide query searched Page 25 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) against nucleotide sequences) to obtain nucleotide sequences homologous to pesticidal- like nucleic acid molecules of the invention, or with the BLASTX program (translated nucleotide query searched against protein sequences) to obtain protein sequences homologous to pesticidal nucleic acid molecules of the invention. BLAST protein searches can be performed with the BLASTP program (protein query searched against protein sequences) to obtain amino acid sequences homologous to pesticidal protein molecules of the invention, or with the TBLASTN program (protein query searched against translated nucleotide sequences) to obtain nucleotide sequences homologous to pesticidal protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized as described in Altschul et al. (1997) Nucleic Acids Res.25:3389. Alternatively, PSI-Blast can be used to perform an iterated search that detects distant relationships between molecules. See Altschul et al. (1997) supra. When utilizing BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., BLASTX and BLASTN) can be used. Alignment may also be performed manually by inspection. Two sequences are "optimally aligned" when they are aligned for similarity scoring using a defined amino acid substitution matrix (e.g., BLOSUM62), gap existence penalty and gap extension penalty so as to arrive at the highest score possible for that pair of sequences. Amino acid substitution matrices and their use in quantifying the similarity between two sequences are well-known in the art and described, e.g., in Dayhoff et al. (1978) "A model of evolutionary change in proteins." In "Atlas of Protein Sequence and Structure," Vol.5, Suppl.3 (ed. M. O. Dayhoff), pp.345-352. Natl. Biomed. Res. Found., Washington, D.C. and Henikoff et al. (1992) Proc. Natl. Acad. Sci. USA 89:10915- 10919. The BLOSUM62 matrix is often used as a default scoring substitution matrix in sequence alignment protocols. The gap existence penalty is imposed for the introduction of a single amino acid gap in one of the aligned sequences, and the gap extension penalty is imposed for each additional empty amino acid position inserted into an already opened gap. The alignment is defined by the amino acid positions of each sequence at which the alignment begins and ends, and optionally by the insertion of a gap or multiple gaps in one or both sequences, so as to arrive at the highest possible score. While optimal alignment and scoring can be accomplished manually, the process is facilitated by the use Page 26 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) of a computer-implemented alignment algorithm, e.g., gapped BLAST 2.0, described in Altschul et al. (1997) Nucleic Acids Res.25:3389-3402, and made available to the public at the National Center for Biotechnology Information Website (www.ncbi.nlm.nih.gov). Optimal alignments, including multiple alignments, can be prepared using, e.g., PSI- BLAST, available through www.ncbi.nlm.nih.gov and described by Altschul et al. (1997) Nucleic Acids Res.25:3389-3402. With respect to an amino acid sequence that is optimally aligned with a reference sequence, an amino acid residue "corresponds to" the position in the reference sequence with which the residue is paired in the alignment. The "position" is denoted by a number that sequentially identifies each amino acid in the reference sequence based on its position relative to the N-terminus. For example, in SEQ ID NO: 2 position 1 is M, position 2 is A, position 3 is Y, etc. When a test sequence is optimally aligned with SEQ ID NO: 2, a residue in the test sequence that aligns with the Y at position 3 is said to "correspond to position 3" of SEQ ID NO: 2. Owing to deletions, insertion, truncations, fusions, etc., that must be taken into account when determining an optimal alignment, in general the amino acid residue number in a test sequence as determined by simply counting from the N-terminal will not necessarily be the same as the number of its corresponding position in the reference sequence. For example, in a case where there is a deletion in an aligned test sequence, there will be no amino acid that corresponds to a position in the reference sequence at the site of deletion. Where there is an insertion in an aligned reference sequence, that insertion will not correspond to any amino acid position in the reference sequence. In the case of truncations or fusions there can be stretches of amino acids in either the reference or aligned sequence that do not correspond to any amino acid in the corresponding sequence. iv. Antibodies Antibodies to the polypeptides of the present invention, or to variants or fragments thereof, are also encompassed. Methods for producing antibodies are well known in the art (see, for example, Harlow and Lane (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.; and U.S. Pat. No. 4,196,265). These antibodies can be used in kits for the detection and isolation of toxin Page 27 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) polypeptides. Thus, this disclosure provides kits comprising antibodies that specifically bind to the polypeptides described herein, including, for example, polypeptides having the sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. II. Pests The compositions and methods provided herein are useful against a variety of pests. "Pests" includes but is not limited to, insects, fungi, bacteria, nematodes, acarids, protozoan pathogens, animal-parasitic liver flukes, and the like. Pests of particular interest are insect pests, particularly insect pests that cause significant damage to agricultural plants. Insect pests include insects selected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, or nematodes. In non-limiting embodiments, the insect pest comprises Western corn rootworm, Diabrotica virgifera virgifera; Fall armyworm, Spodoptera frugiperda; Colorado potato beetle, Leptinotarsa decemlineata; Corn earworm, Helicoverpa zea (in North America same species attacks cotton and called cotton bollworm); European corn borer, Ostrinia nubilalis; Black cutworm, Agrotis ipsilon; Diamondback moth, Plutella xylostella; Velvetbean caterpillar, Anticarsia gemmatalis; Southwestern corn borer, Diatraea grandiosella; Cotton bollworm, Helicoverpa armigera (found other than USA in rest of the world); Southern green stink bug, Nezara viridula; Green stink bug, Chinavia halaris; Brown marmorated stink bug, Halyomorpha halys; and Brown stink bug, Euschistus servus, Euschistus heros (Neotropical brown stink bug OR soy stink bug) ; Piezodorus guildinii (red-banded stink bug); Dichelops melacanthus (no common name) and / or Dichelops furcatus (no common name); an aphid, such as a soybean aphid. In other embodiments, the pest comprises a nematode including, but not limited to, Meloidogyne hapla (Northern root-knot nematode); Meloidogyne enterolobii, Meloidogyne arenaria (peanut root-knot nematode); and Meloidogyne javanica. The term "insect pests" as used herein refers to insects and other similar pests such as, for example, those of the order Acari including, but not limited to, mites and ticks. Insect pests of the present invention include, but are not limited to, insects of the order Lepidoptera, e.g. Achoroia grisella, Acleris gloverana, Acleris variana, Page 28 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophila pometaria, Amyelois transitella, Anagasta kuehniella, Anarsia lineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis, Archips sp., Argyrotaenia sp., Athetis mindara, Bombyx mori, Bucculatrix thurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Colias eurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella, Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphania hyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraea saccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella, Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoellia ambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoa messoria, Galleria mellonella, Grapholita molesta, Harrisina americana, Helicoverpa subflexa, Helicoverpa zea, Heliothis virescens, Hemileuca oliviae, Homoeosoma electellum, Hyphantia cunea, Keiferia lycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellaria lugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Macalla thyrisalis, Malacosoma sp., Mamestra brassicae, Mamestra configurata, Manduca quinquemaculata, Manduca sexta, Maruca testulalis, Melanchra picta, Operophtera brumata, Orgyia sp., Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes, Pectinophora gossypiella, Phryganidia californica, Phyllonorycter blancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynota flouendana, Platynota stultana, Platyptilia carduidactyla, Plodia interpunctella, Plutella xylostella, Pontia protodice, Pseudaletia unipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizura concinna, Sitotroga cerealella, Spilonta ocellana, Spodoptera sp., Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Udea rubigalis, Xylomyges curiails, and Yponomeuta padella. Insect pests also include insects selected from the orders Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, Coleoptera. Insect pests of the invention for the major crops include, but are not limited to: Maize: Ostrinia nubilalis, European corn borer; Agrotis ipsilon, black cutworm; Helicoverpa zeae, corn earworm; Spodoptera frugiperda, fall armyworm; Diatraea grandiosella, southwestern corn borer; Elasmopalpus lignosellus, lesser cornstalk borer; Diatraea saccharalis, surgarcane borer; western corn rootworm, e.g., Diabrotica virgifera virgifera; northern Page 29 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) corn rootworm, e.g., Diabrotica longicornis barberi; southern corn rootworm, e.g., Diabrotica undecimpunctata howardi; Melanotus spp., wireworms; Cyclocephala borealis, northern masked chafer (white grub); Cyclocephala immaculata, southern masked chafer (white grub); Popillia japonica, Japanese beetle; Chaetocnema pulicaria, corn flea beetle; Sphenophorus maidis, maize billbug; Rhopalosiphum maidis, corn leaf aphid; Anuraphis maidiradicis, corn root aphid; Euschistus heros (Neotropical brown stink bug OR soy stink bug) ; Piezodorus guildinii (red-banded stink bug); Dichelops melacanthus (no common name); Dichelops furcatus (no common name) ; Blissus leucopterus leucopterus, chinch bug; Melanoplus femurrubrum, redlegged grasshopper; Melanoplus sanguinipes, migratory grasshopper; Hylemya platura, seedcorn maggot; Agromyza parvicornis, corn blotch leafminer; Anaphothrips obscrurus, grass thrips; Solenopsis milesta, thief ant; Tetranychus urticae, two spotted spider mite; Sorghum: Chilo partellus, sorghum borer; Spodoptera frugiperda, fall armyworm; Helicoverpa zea, corn earworm; Elasmopalpus lignosellus, leser cornstalk borer; Feltia subterranea, granulate cutworm; Phyllophaga crinita, white grub; Eleodes, Conoderus, and Aeolus spp., wireworms; Oulema melanopus, cereal leaf beetle; Chaetocnema pulicaria, corn flea beetle; Sphenophorus maidis, maize billbug; Rhopalosiphum maidis; corn leaf aphid; Sipha flava, yellow sugarcane aphid; chinch bug, e.g., Blissus leucopterus leucopterus; Contarinia sorghicola, sorghum midge; Tetranychus cinnabarinus, carmine spider mite; Tetranychus urticae, two-spotted spider mite; Wheat: Pseudaletia unipunctata, army worm; Spodoptera frugiperda, fall armyworm; Elasmopalpus lignosellus, lesser cornstalk borer; Agrotis orthogonia, pale western cutworm; Elasmopalpus lignosellus, lesser cornstalk borer; Oulema melanopus, cereal leaf beetle; Hypera punctata, clover leaf weevil; southern corn rootworm, e.g., Diabrotica undecimpunctata howardi; Russian wheat aphid; Schizaphis graminum, greenbug; Macrosiphum avenae, English grain aphid; Melanoplus femurrubrum, redlegged grasshopper; Melanoplus differentialis, differential grasshopper; Melanoplus sanguinipes, migratory grasshopper; Mayetiola destructor, Hessian fly; Sitodiplosis mosellana, wheat midge; Meromyza americana, wheat stem maggot; Hylemya coarctata, wheat bulb fly; Frankliniella fusca, tobacco thrips; Cephus cinctus, wheat stem sawfly; Aceria tulipae, wheat curl mite; Sunflower: Cylindrocupturus adspersus, sunflower stem weevil; Smicronyx fulus, red sunflower seed Page 30 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) weevil; Smicronyx sordidus, gray sunflower seed weevil; Suleima helianthana, sunflower bud moth; Homoeosoma electellum, sunflower moth; Zygogramma exclamationis, sunflower beetle; Bothyrus gibbosus, carrot beetle; Neolasioptera murtfeldtiana, sunflower seed midge; Cotton: Heliothis virescens, tobacco budworm; Helicoverpa zea, cotton bollworm; Spodoptera exigua, beet armyworm; Pectinophora gossypiella, pink bollworm; boll weevil, e.g., Anthonomus grandis; Aphis gossypii, cotton aphid; Pseudatomoscelis seriatus, cotton fleahopper; Trialeurodes abutilonea, bandedwinged whitefly; Lygus lineolaris, tarnished plant bug; Melanoplus femurrubrum, redlegged grasshopper; Melanoplus differentialis, differential grasshopper; Thrips tabaci, onion thrips; Franklinkiella fusca, tobacco thrips; Tetranychus cinnabarinus, carmine spider mite; Tetranychus urticae, two-spotted spider mite; Rice: Diatraea saccharalis, sugarcane borer; Spodoptera frugiperda, fall armyworm; Helicoverpa zea, corn earworm; Colaspis brunnea, grape colaspis; Lissorhoptrus oryzophilus, rice water weevil; Sitophilus oryzae, rice weevil; Nephotettix nigropictus, rice leafhoper; chinch bug, e.g., Blissus leucopterus leucopterus; Acrosternum hilare, green stink bug; Soybean: Pseudoplusia includens, soybean looper; Anticarsia gemmatalis, velvetbean caterpillar; Plathypena scabra, green cloverworm; Ostrinia nubilalis, European corn borer; Agrotis ipsilon, black cutworm; Spodoptera exigua, beet armyworm; Heliothis virescens, tobacco budworm; Helicoverpa zea, cotton bollworm; Epilachna varivestis, Mexican bean beetle; Myzus persicae, green peach aphid; Empoasca fabae, potato leafhopper; Acrosternum hilare, green stink bug; Melanoplus femurrubrum, redlegged grasshopper; Melanoplus differentialis, differential grasshopper; Hylemya platura, seedcorn maggot; Sericothrips variabilis, soybean thrips; Thrips tabaci, onion thrips; Tetranychus turkestani, strawberry spider mite; Tetranychus urticae, two-spotted spider mite; Barley: Ostrinia nubilalis, European corn borer; Agrotis ipsilon, black cutworm; Schizaphis graminum, greenbug; chinch bug, e.g., Blissus leucopterus leucopterus; Acrosternum hilare, green stink bug; Euschistus servus, brown stink bug; Jylemya platura, seedcorn maggot; Mayetiola destructor, Hessian fly; Petrobia latens, brown wheat mite; Oil Seed Rape: Vrevicoryne brassicae, cabbage aphid; Phyllotreta cruciferae, crucifer flea beetle; Phyllotreta striolata, striped flea beetle; Phyllotreta nemorum, striped turnip flea beetle; Meligethes aeneus, rapeseed beetle; and the pollen beetles Meligethes rufimanus, Meligethes Page 31 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) nigrescens, Meligethes canadianus, and Meligethes viridescens; Potato: Leptinotarsa decemlineata, Colorado potato beetle. The methods and compositions provided herein may be effective against Hemiptera such as Lygus hesperus, Lygus lineolaris, Lygus pratensis, Lygus rugulipennis Popp, Lygus pabulinus, Calocoris norvegicus, Orthops compestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltis notatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis, Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus, Poecilocapsus lineatus, Blissus leucopterus, Nysius ericae, Nysius raphanus, Euschistus servus, Nezara viridula, Eurygaster, Coreidae, Pyrrhocoridae, Tinidae, Blostomatidae, Reduviidae, and Cimicidae. Pests of interest also include Araecerus fasciculatus, coffee bean weevil; Acanthoscelides obtectus, bean weevil; Bruchus rufmanus, broadbean weevil; Bruchus pisorum, pea weevil; Zabrotes subfasciatus, Mexican bean weevil; Diabrotica balteata, banded cucumber beetle; Cerotoma trifurcata, bean leaf beetle; Diabrotica virgifera, Mexican corn rootworm; Epitrix cucumeris, potato flea beetle; Chaetocnema confinis, sweet potato flea beetle; Hypera postica, alfalfa weevil; Anthonomus quadrigibbus, apple curculio; Sternechus paludatus, bean stalk weevil; Hypera brunnipennis, Egyptian alfalfa weevil; Sitophilus granaries, granary weevil; Craponius inaequalis, grape curculio; Sitophilus zeamais, maize weevil; Conotrachelus nenuphar, plum curculio; Euscepes postfaciatus, West Indian sweet potato weevil; Maladera castanea, Asiatic garden beetle; Rhizotrogus majalis, European chafer; Macrodactylus subspinosus, rose chafer; Tribolium confusum, confused flour beetle; Tenebrio obscurus, dark mealworm; Tribolium castaneum, red flour beetle; Tenebrio molitor, yellow mealworm. In some embodiments, the presently disclosed pesticidal proteins have pesticidal activity against insect pests that are resistant to one or more strains of Bacillus thuringiensis or one or more toxin proteins produced by one or more strains of Bacillus thuringiensis. As used herein, the term “resistant” as it relates to an insect pest refers to an insect pest that does not die in the presence of a toxin or does not exhibit reduced growth in the presence of a toxin when compared to the growth of the insect pest in the absence of the toxin. In certain embodiments, the presently disclosed pesticidal proteins have pesticidal activity against insect pests that are resistant to any one of Cry34 / 35 and Page 32 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Cry3Bb. In particular embodiments, the presently disclosed pesticidal proteins have pesticidal activity against Colepteran insect pests (including, but not limited to, Western corn rootworm) that are resistant to one or more of Cry34 / 35 or Cry3Bb. Nematodes include parasitic nematodes such as root-knot, cyst, and lesion nematodes, including Heterodera spp., Meloidogyne spp., and Globodera spp.; particularly members of the cyst nematodes, including, but not limited to, Heterodera glycines (soybean cyst nematode); Heterodera schachtii (beet cyst nematode); Heterodera avenae (cereal cyst nematode); and Globodera rostochiensis and Globodera pailida (potato cyst nematodes). Lesion nematodes include Pratylenchus spp. Insect pests may be tested for pesticidal activity of compositions of the invention in early developmental stages, e.g., as larvae or other immature forms. The insects may be reared in total darkness at from about 20º C to about 30º C and from about 30% to about 70% relative humidity. Bioassays may be performed as described in Czapla and Lang (1990) J. Econ. Entomol.83 (6): 2480-2485. See, also the experimental section herein. III. Expression Cassettes Polynucleotides encoding the pesticidal proteins provided herein can be provided in expression cassettes for expression in an organism of interest. The cassette will include 5' and 3' regulatory sequences operably linked to a polynucleotide encoding a pesticidal polypeptide provided herein that allows for expression of the polynucleotide. The cassette may additionally contain at least one additional gene or genetic element to be cotransformed into the organism. Where additional genes or elements are included, the components are operably linked. Alternatively, the additional gene(s) or element(s) can be provided on multiple expression cassettes. Such an expression cassette is provided with a plurality of restriction sites and / or recombination sites for insertion of the polynucleotides to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain a selectable marker gene. In one embodiment, any one of the plyA sequences provided herein is transformed into the cell or organism of interest. In another embodiment, any one of the plyB sequences provided herein is transformed into the cell or organism of interest. In Page 33 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) another embodiment, a plyA sequence and a plyB sequence provided herein are cotransformed into the cell or organism of interest. In some embodiments, the ply A and plyB sequences are on separate DNA constructs, and in other embodiments, the plyA and plyB sequences are on the same DNA construct. The expression cassette will include in the 5'-3' direction of transcription, a transcriptional and translational initiation region (i.e., a promoter), a pesticidal polynucleotide of the invention, and a transcriptional and translational termination region (i.e., termination region) functional in the organism of interest, i.e., a plant or bacteria. The promoters of the invention are capable of directing or driving expression of a coding sequence in a host cell. The regulatory regions (i.e., promoters, transcriptional regulatory regions, and translational termination regions) may be endogenous or heterologous to the host cell or to each other. As used herein, “heterologous” in reference to a sequence is a sequence that originates from a foreign species, or, if from the same species, is substantially modified from its native form in composition and / or genomic locus by deliberate human intervention. As used herein, a chimeric gene comprises a coding sequence operably linked to a transcription initiation region that is heterologous to the coding sequence. Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also Guerineau et al. (1991) Mol. Gen. Genet.262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) Genes Dev.5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res.17:7891-7903; and Joshi et al. (1987) Nucleic Acids Res.15:9627-9639. Additional regulatory signals include, but are not limited to, transcriptional initiation start sites, operators, activators, enhancers, other regulatory elements, ribosomal binding sites, an initiation codon, termination signals, and the like. See, for example, U.S. Pat. Nos.5,039,523 and 4,853,331; EPO 0480762A2; Sambrook et al. (1992) Molecular Cloning: A Laboratory Manual, ed. Maniatis et al. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), hereinafter "Sambrook 11"; Davis et al., eds. (1980) Advanced Bacterial Genetics (Cold Spring Harbor Laboratory Press), Cold Spring Harbor, N.Y., and the references cited therein. Page 34 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) In preparing the expression cassette, the various DNA fragments may be manipulated, so as to provide for the DNA sequences in the proper orientation and, as appropriate, in the proper reading frame. Toward this end, adapters or linkers may be employed to join the DNA fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous DNA, removal of restriction sites, or the like. For this purpose, in vitro mutagenesis, primer repair, restriction, annealing, resubstitutions, e.g., transitions and transversions, may be involved. A number of promoters can be used in the practice of the invention. The promoters can be selected based on the desired outcome. The nucleic acids can be combined with constitutive, inducible, tissue-preferred, or other promoters for expression in the organism of interest. See, for example, promoters set forth in WO 99 / 43838 and in US Patent Nos: 8,575,425; 7,790,846; 8,147,856; 8,586832; 7,772,369; 7,534,939; 6,072,050; 5,659,026; 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611; herein incorporated by reference. For expression in plants, constitutive promoters also include CaMV 35S promoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroy et al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al. (1989) Plant Mol. Biol.12:619-632 and Christensen et al. (1992) Plant Mol. Biol.18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet.81:581-588); MAS (Velten et al. (1984) EMBO J.3:2723-2730). Inducible promoters include those that drive expression of pathogenesis-related proteins (PR proteins), which are induced following infection by a pathogen. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol.89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; and Van Loon (1985) Plant Mol. Virol.4:111-116; and WO 99 / 43819, herein incorporated by reference. Promoters that are expressed locally at or near the site of pathogen infection may also be used (Marineau et al. (1987) Plant Mol. Biol.9:335-342; Matton et al. (1989) Molecular Plant-Microbe Interactions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci. USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet.2:93-98; and Yang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977; Chen et al. (1996) Plant J.10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci. USA 91:2507- 2511; Warner et al. (1993) Plant J.3:191-201; Siebertz et al. (1989) Plant Cell 1:961- Page 35 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 968; Cordero et al. (1992) Physiol. Mol. Plant Path.41:189-200; U.S. Patent No. 5,750,386 (nematode-inducible); and the references cited therein). Wound-inducible promoters may be used in the constructions of the invention. Such wound-inducible promoters include pin II promoter (Ryan (1990) Ann. Rev. Phytopath.28:425-449; Duan et al. (1996) Nature Biotechnology 14:494-498); wun1 and wun2 (U.S. Patent No.5,428,148); win1 and win2 (Stanford et al. (1989) Mol. Gen. Genet.215:200-208); systemin (McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al. (1993) Plant Mol. Biol.22:783-792; Eckelkamp et al. (1993) FEBS Letters 323:73-76); MPI gene (Corderok et al. (1994) Plant J.6(2):141-150); and the like, herein incorporated by reference. Tissue-preferred promoters for use in the invention include those set forth in Yamamoto et al. (1997) Plant J.12(2):255-265; Kawamata et al. (1997) Plant Cell Physiol.38(7):792-803; Hansen et al. (1997) Mol. Gen Genet.254(3):337-343; Russell et al. (1997) Transgenic Res.6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341; Van Camp et al. (1996) Plant Physiol.112(2):525-535; Canevascini et al. (1996) Plant Physiol.112(2):513-524; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ.20:181-196; Orozco et al. (1993) Plant Mol Biol.23(6):1129-1138; Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; and Guevara-Garcia et al. (1993) Plant J.4(3):495-505. Leaf-preferred promoters include those set forth in Yamamoto et al. (1997) Plant J.12(2):255-265; Kwon et al. (1994) Plant Physiol.105:357-67; Yamamoto et al. (1994) Plant Cell Physiol.35(5):773-778; Gotor et al. (1993) Plant J.3:509-18; Orozco et al. (1993) Plant Mol. Biol.23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci. USA 90(20):9586-9590. Root-preferred promoters are known and include those in Hire et al. (1992) Plant Mol. Biol.20(2):207-218 (soybean root-specific glutamine synthetase gene); Keller and Baumgartner (1991) Plant Cell 3(10):1051-1061 (root-specific control element); Sanger et al. (1990) Plant Mol. Biol.14(3):433-443 (mannopine synthase (MAS) gene of Agrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):11-22 (cytosolic glutamine synthetase (GS)); Bogusz et al. (1990) Plant Cell 2(7):633-641; Leach and Aoyagi (1991) Plant Science (Limerick) 79(1):69-76 (rolC and rolD); Teeri et al. (1989) Page 36 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) EMBO J.8(2):343-350; Kuster et al. (1995) Plant Mol. Biol.29(4):759-772 (the VfENOD-GRP3 gene promoter); and, Capana et al. (1994) Plant Mol. Biol.25(4):681- 691 (rolB promoter). See also U.S. Patent Nos.5,837,876; 5,750,386; 5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179. "Seed-preferred" promoters include both "seed-specific" promoters (those promoters active during seed development such as promoters of seed storage proteins) as well as "seed-germinating" promoters (those promoters active during seed germination). See Thompson et al. (1989) BioEssays 10:108. Seed-preferred promoters include, but are not limited to, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); milps (myo-inositol-1-phosphate synthase) (see WO 00 / 11177 and U.S. Patent No.6,225,529). Gamma-zein is an endosperm-specific promoter. Globulin 1 (Glb-1) is a representative embryo-specific promoter. For dicots, seed-specific promoters include, but are not limited to, bean β-phaseolin, napin, β-conglycinin, soybean lectin, cruciferin, and the like. For monocots, seed-specific promoters include, but are not limited to, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, gamma-zein, waxy, shrunken 1, shrunken 2, Globulin 1, etc. See also WO 00 / 12733, where seed-preferred promoters from end1 and end2 genes are disclosed. For expression in a bacterial host, promoters that function in bacteria are well- known in the art. Such promoters include any of the known crystal protein gene promoters, including the promoters of any of the pesticidal proteins of the invention, and promoters specific for B. thuringiensis sigma factors. Alternatively, mutagenized or recombinant crystal protein-encoding gene promoters may be recombinantly engineered and used to promote expression of the novel gene segments disclosed herein. The expression cassette can also comprise a selectable marker gene for the selection of transformed cells. Selectable marker genes are utilized for the selection of transformed cells or tissues. Marker genes include genes encoding antibiotic resistance, such as those encoding neomycin phosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), as well as genes conferring resistance to herbicidal compounds, such as glufosinate ammonium, bromoxynil, imidazolinones, and 2,4- dichlorophenoxyacetate (2,4-D). Additional selectable markers are known and any can be used. See, for example, US Provisional application 62 / 094,697, filed on December 19, Page 37 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 2014, and US Provisional Application 62 / 189,505, filed July 7, 2015, both of which are herein incorporated by reference in their entirety, which discloses glufosinate resistance sequences that can be employed as selectable markers. See, for example, PCT / US2015 / 066648, filed on December 18, 2015, herein incorporated by reference in its entirety, which discloses glufosinate resistance sequences that can be employed as selectable markers. IV. Methods, Host Cells and Plant Cells As indicated, DNA constructs comprising nucleotide sequences encoding the pesticidal proteins or active variants or fragments thereof can be used to transform plants of interest or other organisms of interest. Methods for transformation involve introducing a nucleotide construct into a plant. By "introducing" is intended to introduce the nucleotide construct to the plant or other host cell in such a manner that the construct gains access to the interior of a cell of the plant or host cell. The methods of the invention do not require a particular method for introducing a nucleotide construct to a plant or host cell, only that the nucleotide construct gains access to the interior of at least one cell of the plant or the host organism. Methods for introducing nucleotide constructs into plants and other host cells are known in the art including, but not limited to, stable transformation methods, transient transformation methods, and virus-mediated methods. The methods result in a transformed organism, such as a plant, including whole plants, as well as plant organs (e.g., leaves, stems, roots, etc.), seeds, plant cells, propagules, embryos and progeny of the same. Plant cells can be differentiated or undifferentiated (e.g., callus, suspension culture cells, protoplasts, leaf cells, root cells, phloem cells, pollen). "Transgenic plants" or "transformed plants" or "stably transformed" plants or cells or tissues refers to plants that have incorporated or integrated a polynucleotide encoding at least one pesticidal polypeptide of the invention. It is recognized that other exogenous or endogenous nucleic acid sequences or DNA fragments may also be incorporated into the plant cell. Agrobacterium-and biolistic-mediated transformation remain the two predominantly employed approaches. However, transformation may be performed by Page 38 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) infection, transfection, microinjection, electroporation, microprojection, biolistics or particle bombardment, electroporation, silica / carbon fibers, ultrasound mediated, PEG mediated, calcium phosphate co-precipitation, polycation DMSO technique, DEAE dextran procedure, Agro and viral mediated (Caulimoriviruses, Geminiviruses, RNA plant viruses), liposome mediated and the like. Transformation protocols as well as protocols for introducing polypeptides or polynucleotide sequences into plants may vary depending on the type of plant or plant cell, i.e., monocot or dicot, targeted for transformation. Methods for transformation are known in the art and include those set forth in US Patent Nos: 8,575,425; 7,692,068; 8,802,934; 7,541,517; each of which is herein incorporated by reference. See, also, Rakoczy-Trojanowska, M. (2002) Cell Mol Biol Lett.7:849-858; Jones et al. (2005) Plant Methods 1:5; Rivera et al. (2012) Physics of Life Reviews 9:308-345; Bartlett et al. (2008) Plant Methods 4:1-12; Bates, G.W. (1999) Methods in Molecular Biology 111:359-366; Binns and Thomashow (1988) Annual Reviews in Microbiology 42:575- 606; Christou, P. (1992) The Plant Journal 2:275-281; Christou, P. (1995) Euphytica 85:13-27; Tzfira et al. (2004) TRENDS in Genetics 20:375-383; Yao et al. (2006) Journal of Experimental Botany 57:3737-3746; Zupan and Zambryski (1995) Plant Physiology 107:1041-1047; Jones et al. (2005) Plant Methods 1:5; Transformation may result in stable or transient incorporation of the nucleic acid into the cell. "Stable transformation" is intended to mean that the nucleotide construct introduced into a host cell integrates into the genome of the host cell and is capable of being inherited by the progeny thereof. "Transient transformation" is intended to mean that a polynucleotide is introduced into the host cell and does not integrate into the genome of the host cell. Methods for transformation of chloroplasts are known in the art. See, for example, Svab et al. (1990) Proc. Nail. Acad. Sci. USA 87:8526-8530; Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab and Maliga (1993) EMBO J.12:601-606. The method relies on particle gun delivery of DNA containing a selectable marker and targeting of the DNA to the plastid genome through homologous recombination. Additionally, plastid transformation can be accomplished by transactivation of a silent plastid-borne transgene by tissue-preferred expression of a nuclear-encoded and plastid- Page 39 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) directed RNA polymerase. Such a system has been reported in McBride et al. (1994) Proc. Natl. Acad. Sci. USA 91:7301-7305. The cells that have been transformed may be grown into plants in accordance with conventional ways. See, for example, McCormick et al. (1986) Plant Cell Reports 5:81- 84. These plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting hybrid having constitutive expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure expression of the desired phenotypic characteristic has been achieved. In this manner, the present invention provides transformed seed (also referred to as "transgenic seed") having a nucleotide construct of the invention, for example, an expression cassette of the invention, stably incorporated into their genome. In specific embodiments, the sequences provide herein can be targeted to specific cite within the genome of the host cell or plant cell. Such methods include, but are not limited to, meganucleases designed against the plant genomic sequence of interest (D’Halluin et al.2013 Plant Biotechnol J); CRISPR-Cas9, TALENs, and other technologies for precise editing of genomes (Feng, et al. Cell Research 23:1229-1232, 2013, Podevin, et al. Trends Biotechnology, online publication, 2013, Wei et al., J Gen Genomics, 2013, Zhang et al (2013) WO 2013 / 026740); Cre-lox site-specific recombination (Dale et al. (1995) Plant J 7:649-659; Lyznik, et al. (2007) Transgenic Plant J 1:1-9; FLP-FRT recombination (Li et al. (2009) Plant Physiol 151:1087-1095); Bxb1-mediated integration (Yau et al. Plant J (2011) 701:147-166); zinc-finger mediated integration (Wright et al. (2005) Plant J 44:693-705); Cai et al. (2009) Plant Mol Biol 69:699-709); and homologous recombination (Lieberman-Lazarovich and Levy (2011) Methods Mol Biol 701: 51-65); Puchta (2002) Plant Mol Biol 48:173-182). The sequence provided herein may be used for transformation of any plant species, including, but not limited to, monocots and dicots. Examples of plants of interest include, but are not limited to, corn (maize), sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape, Brassica sp., alfalfa, rye, millet, safflower, peanuts, sweet potato, Page 40 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) cassava, coffee, coconut, pineapple, citrus trees, cocoa, tea, banana, avocado, fig, guava, mango, olive, papaya, cashew, macadamia, almond, oats, vegetables, ornamentals, and conifers. Vegetables include, but are not limited to, tomatoes, lettuce, green beans, lima beans, peas, and members of the genus Curcumis such as cucumber, cantaloupe, and musk melon. Ornamentals include, but are not limited to, azalea, hydrangea, hibiscus, roses, tulips, daffodils, petunias, carnation, poinsettia, and chrysanthemum. Preferably, plants of the present invention are crop plants (for example, maize, sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, oilseed rape, etc.). As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips, anthers, and the like. Grain is intended to mean the mature seed produced by commercial growers for purposes other than growing or reproducing the species. Progeny, variants, and mutants of the regenerated plants are also included within the scope of the invention, provided that these parts comprise the introduced polynucleotides. Further provided is a processed plant product or byproduct that retains the sequences disclosed herein, including for example, soymeal. In another embodiment, the genes encoding the pesticidal proteins can be used to transform organism and thereby create insect pathogenic organisms. Such organisms include baculoviruses, fungi, protozoa, bacteria, and nematodes. Microorganism hosts that are known to occupy the "phytosphere" (phylloplane, phyllosphere, rhizosphere, and / or rhizoplana) of one or more crops of interest may be selected. These microorganisms are selected so as to be capable of successfully competing in the particular environment with the wild-type microorganisms, provide for stable maintenance and expression of the gene expressing the pesticidal protein, and desirably, provide for improved protection of the pesticide from environmental degradation and inactivation. Page 41 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Such microorganisms include archaea, bacteria, algae, and fungi. Of particular interest are microorganisms such as bacteria, e.g., Bacillus, Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces, Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter, Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes. Fungi include yeast, e.g., Saccharomyces, Cryptococcus, Kluyveromyces, Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interest are such phytosphere bacterial species as Pseudomonas syringae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens, Acetobacter xylinum, Agrobacteria, Rhodopseudomonas spheroides, Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus, Clavibacter xyli and Azotobacter vinlandir and phytosphere yeast species such as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca, Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei, S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus, Kluyveromyces veronae, Aureobasidium pollulans, Bacillus thuringiensis, Escherichia coli, Bacillus subtilis, and the like.   Illustrative prokaryotes, both Gram-negative and gram-positive, include Enterobacteriaceae, such as Escherichia, Erwinia, Shigella, Salmonella, and Proteus; Bacillaceae; Rhizobiceae, such as Rhizobium; Spirillaceae, such as photobacterium, Zymomonas, Serratia, Aeromonas, Vibrio, Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, such as Pseudomonas and Acetobacter; Azotobacteraceae and Nitrobacteraceae. Fungi include Phycomycetes and Ascomycetes, e.g., yeast, such as Saccharomyces and Schizosaccharomyces; and Basidiomycetes yeast, such as Rhodotorula, Aureobasidium, Sporobolomyces, and the like. Genes encoding pesticidal proteins can be introduced by means of electrotransformation, PEG induced transformation, heat shock, transduction, conjugation, and the like. Specifically, genes encoding the pesticidal proteins can be cloned into a shuttle vector, for example, pHT3101 (Lerecius et al. (1989) FEMS Microbiol. Letts.60: 211-218. The shuttle vector pHT3101 containing the coding sequence for the particular pesticidal protein gene can, for example, be transformed into the root-colonizing Bacillus by means of electroporation (Lerecius et al. (1989) FEMS Microbiol. Letts.60: 211-218). Page 42 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Expression systems can be designed so that pesticidal proteins are secreted outside the cytoplasm of gram-negative bacteria by fusing an appropriate signal peptide to the amino-terminal end of the pesticidal protein. Signal peptides recognized by E. coli include the OmpA protein (Ghrayeb et al. (1984) EMBO J, 3: 2437-2442). Pesticidal proteins and active variants thereof can be fermented in a bacterial host and the resulting bacteria processed and used as a microbial spray in the same manner that Bacillus thuringiensis strains have been used as insecticidal sprays. In the case of a pesticidal protein(s) that is secreted from Bacillus, the secretion signal is removed or mutated using procedures known in the art. Such mutations and / or deletions prevent secretion of the pesticidal protein(s) into the growth medium during the fermentation process. The pesticidal proteins are retained within the cell, and the cells are then processed to yield the encapsulated pesticidal proteins. Alternatively, the pesticidal proteins are produced by introducing heterologous genes into a cellular host or through the expression of the pesticidal protein in its native cell. Expression of the heterologous gene or the native gene results, directly or indirectly, in the intracellular production and maintenance of the pesticide. These cells are then treated under conditions that prolong the activity of the toxin produced in the cell when the cell is applied to the environment of target pest(s). The resulting product retains the toxicity of the toxin. These pesticidal proteins may then be formulated in accordance with conventional techniques for application to the environment hosting a target pest, e.g., soil, water, and foliage of plants. See, for example U.S. Patent No.6,468,523 and U.S. Publication No.20050138685, and the references cited therein. In the present invention, a transformed microorganism or the native microorganism (which includes whole organisms, cells, spore(s), pesticidal protein(s), pesticidal component(s), pest-impacting component(s), mutant(s), living or dead cells and cell components, including mixtures of living and dead cells and cell components, and including broken cells and cell components) or an isolated pesticidal protein can be prepared as a formulation and can be formulated with an acceptable carrier into a pesticidal or agricultural composition(s) that is, for example, a liquid, a suspension, a solution, an emulsion, a powder, a dusting powder, dust, pellet, granule, a dispersible granule, a wettable powder, a dry flowable, a disbursable flowable, a wettable granule, a spray dried cellular composition, an Page 43 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) emulsifiable concentrate, an aerosol, an impregnated granule, an adjuvant, a coatable paste, spray, colloid, an aqueous solution, an oil-based solution, and also encapsulations in, for example, polymer substances. Agricultural compositions may comprise a polypeptide, a recombinogenic polypeptide or a variant or fragment thereof, as disclosed herein or a heterologous microbe expressing the pesticidal polypeptide or the native microbe comprising the pesticidal protein. The agricultural composition disclosed herein may be applied to the environment of a plant or an area of cultivation, or applied to the plant, plant part, plant cell, or seed. Agricultural compositions may comprise a combination of any of the plyA and any of the plyB polypeptides or variants or fragments thereof provided herein. Such agricultural compositions comprising both plyA and plyB polypeptides may be applied to the environment of a plant or an area of cultivation, or applied to the plant, plant part, plant cell, or seed. In other embodiments, the plyA polypeptides provided herein may be applied as a separate composition from the plyB polypeptides provided herein. In some embodiments the plyA and plyB polypeptides provided herein are applied to the environment of a plant or an area of cultivation, or applied to the plant, plant part, plant cell, or seed in separate compositions. Such compositions can be applied sequentially or simultaneously. Such compositions disclosed above may further comprise the addition of a surface-active agent, an inert carrier, a preservative, a humectant, a feeding stimulant, an attractant, an encapsulating agent, a binder, an emulsifier, a dye, a UV protectant, a buffer, a flow agent or fertilizers, micronutrient donors, or other preparations that influence plant growth. One or more agrochemicals including, but not limited to, herbicides, insecticides, fungicides, bactericides, nematicides, molluscicides, acaracides, plant growth regulators, harvest aids, and fertilizers, can be combined with carriers, surfactants or adjuvants customarily employed in the art of formulation or other components to facilitate product handling and application for particular target pests. Suitable carriers and adjuvants can be solid or liquid and correspond to the substances ordinarily employed in formulation technology, e.g., natural or regenerated mineral substances, solvents, dispersants, wetting agents, tackifiers, binders, or fertilizers. The Page 44 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) active ingredients of the present invention are normally applied in the form of compositions and can be applied to the crop area, plant, or seed to be treated. For example, the compositions of the present invention may be applied to grain in preparation for or during storage in a grain bin or silo, etc. The compositions of the present invention may be applied simultaneously or in succession with other compounds. Methods of applying an active ingredient of the present invention or an agrochemical composition of the present invention that contains at least one of the pesticidal proteins produced by the bacterial strains of the present invention include, but are not limited to, foliar application, seed coating, and soil application. The number of applications and the rate of application depend on the intensity of infestation by the corresponding pest. Suitable surface-active agents include, but are not limited to, anionic compounds such as a carboxylate of, for example, a metal; a carboxylate of a long chain fatty acid; an N-acylsarcosinate; mono or di-esters of phosphoric acid with fatty alcohol ethoxylates or salts of such esters; fatty alcohol sulfates such as sodium dodecyl sulfate, sodium octadecyl sulfate or sodium cetyl sulfate; ethoxylated fatty alcohol sulfates; ethoxylated alkylphenol sulfates; lignin sulfonates; petroleum sulfonates; alkyl aryl sulfonates such as alkyl-benzene sulfonates or lower alkylnaphtalene sulfonates, e.g., butyl-naphthalene sulfonate; salts of sulfonated naphthalene-formaldehyde condensates; salts of sulfonated phenol-formaldehyde condensates; more complex sulfonates such as the amide sulfonates, e.g., the sulfonated condensation product of oleic acid and N-methyl taurine; or the dialkyl sulfosuccinates, e.g., the sodium sulfonate of dioctyl succinate. Non-ionic agents include condensation products of fatty acid esters, fatty alcohols, fatty acid amides or fatty-alkyl- or alkenyl-substituted phenols with ethylene oxide, fatty esters of polyhydric alcohol ethers, e.g., sorbitan fatty acid esters, condensation products of such esters with ethylene oxide, e.g., polyoxyethylene sorbitar fatty acid esters, block copolymers of ethylene oxide and propylene oxide, acetylenic glycols such as 2,4,7,9- tetraethyl-5-decyn-4,7-diol, or ethoxylated acetylenic glycols. Examples of a cationic surface-active agent include, for instance, an aliphatic mono-, di-, or polyamine such as an acetate, naphthenate or oleate; or oxygen-containing amine such as an amine oxide of polyoxyethylene alkylamine; an amide-linked amine prepared by the condensation of a carboxylic acid with a di- or polyamine; or a quaternary ammonium salt. Page 45 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Examples of inert materials include but are not limited to inorganic minerals such as kaolin, phyllosilicates, carbonates, sulfates, phosphates, or botanical materials such as cork, powdered corncobs, peanut hulls, rice hulls, and walnut shells. The compositions of the present invention can be in a suitable form for direct application or as a concentrate of primary composition that requires dilution with a suitable quantity of water or other diluent before application. The pesticidal concentration will vary depending upon the nature of the particular formulation, specifically, whether it is a concentrate or to be used directly. The composition contains 1 to 98% of a solid or liquid inert carrier, and 0 to 50% or 0.1 to 50% of a surfactant. These compositions will be administered at the labeled rate for the commercial product, for example, about 0.01 lb-5.0 lb. per acre when in dry form and at about 0.01 pts.-10 pts. per acre when in liquid form. In a further embodiment, the compositions, as well as the transformed microorganisms and pesticidal proteins, provided herein can be treated prior to formulation to prolong the pesticidal activity when applied to the environment of a target pest as long as the pretreatment is not deleterious to the pesticidal activity. Such treatment can be by chemical and / or physical means as long as the treatment does not deleteriously affect the properties of the composition(s). Examples of chemical reagents include but are not limited to halogenating agents; aldehydes such as formaldehyde and glutaraldehyde; anti-infectives, such as zephiran chloride; alcohols, such as isopropanol and ethanol; and histological fixatives, such as Bouin's fixative and Helly's fixative (see, for example, Humason (1967) Animal Tissue Techniques (W.H. Freeman and Co.). The terms “controlling” or “control” with regards to a plant pest refers to one or more of inhibiting or reducing the growth, feeding, fecundity, reproduction, and / or proliferation of a plant pest or killing (e.g., causing the morbidity or mortality, or reduced fecundity) of a plant pest. As such, a plant treated with a pesticidal polypeptide or protein, a composition comprising a pesticidal polypeptide or protein, and / or expressing a pesticidal polypeptide or protein provided herein may show a reduced infestation of pests, or reduced damage caused by pests by a statistically significant amount. In particular embodiments, “controlling” and “protecting” a plant from a pest refers to one or more of inhibiting or reducing the growth, germination, reproduction, and / or Page 46 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) proliferation of a pest; and / or killing, removing, destroying, or otherwise diminishing the occurrence, and / or activity of a pest. As such, a plant treated with a pesticidal protein provided herein and / or a plant expressing a pesticidal protein provided herein may show a reduced severity or reduced development of disease or damage in the presence of plant pests by a statistically significant amount. Provided herein are methods of controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof a nucleic acid molecule that encodes a pesticidal polypeptide provided herein. Also provided are methods of controlling a plant pest and / or damage caused by a plant pest comprising applying to a plant having a plant pest and / or damage an effective amount of at least one pesticidal polypeptide provided herein or an active variant thereof, and / or a composition derived therefrom wherein the pesticidal polypeptide and / or the composition derived therefrom controls a plant pest that causes the plant disease or damage. In particular embodiments, the plant damage is caused by an insect pest. In one aspect, pests may be killed or reduced in numbers in a given area by application of the pesticidal proteins provided herein to the area. Alternatively, the pesticidal proteins may be prophylactically applied to an environmental area to prevent infestation by a susceptible pest. Preferably the pest ingests, or is contacted with, a pesticidally-effective amount of the polypeptide. By "pesticidally-effective amount" is intended an amount of the pesticide that is able to bring about death to at least one pest, or to noticeably reduce pest growth, feeding, or normal physiological development. This amount will vary depending on such factors as, for example, the specific target pests to be controlled, the specific environment, location, plant, crop, or agricultural site to be treated, the environmental conditions, and the method, rate, concentration, stability, and quantity of application of the pesticidally-effective polypeptide composition. The formulations or compositions may also vary with respect to climatic conditions, environmental considerations, and / or frequency of application and / or severity of pest infestation. The active ingredients are normally applied in the form of compositions and can be applied to the crop area, plant, or seed to be treated. Methods are therefore provided for providing to a plant, plant cell, seed, plant part or an area of cultivation, an effective Page 47 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) amount of the agricultural composition comprising the polypeptide, recombinogenic polypeptide or an active variant or fragment thereof. By “effective amount” is intended an amount of a protein or composition has pesticidal activity that is sufficient to kill or control the pest or result in a noticeable reduction in pest growth, feeding, or normal physiological development. Such decreases in numbers, pest growth, feeding or normal development can comprise any statistically significant decrease, including, for example a decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. For example, the compositions may be applied to grain in preparation for or during storage in a grain bin or silo, etc. The compositions may be applied simultaneously or in succession with other compounds. Methods of applying an active ingredient or an agrochemical composition comprising at least one of the polypeptides, recombinogenic polypeptides or variants or fragments thereof as disclosed herein, include but are not limited to, foliar application, seed coating, and soil application. Methods for increasing plant yield are provided. The methods comprise providing a plant or plant cell expressing a polynucleotide encoding the pesticidal polypeptide sequence disclosed herein and growing the plant or a seed thereof in a field infested with (or susceptible to infestation by) a pest against which said polypeptide has pesticidal activity. In some embodiments, the polypeptide has pesticidal activity against a lepidopteran, coleopteran, dipteran, hemipteran, or nematode pest, and said field is infested with a lepidopteran, hemipteran, coleopteran, dipteran, or nematode pest. As defined herein, the "yield" of the plant refers to the quality and / or quantity of biomass produced by the plant. By "biomass" is intended any measured plant product. An increase in biomass production is any improvement in the yield of the measured plant product. Increasing plant yield has several commercial applications. For example, increasing plant leaf biomass may increase the yield of leafy vegetables for human or animal consumption. Additionally, increasing leaf biomass can be used to increase production of plant-derived pharmaceutical or industrial products. An increase in yield can comprise any statistically significant increase including, but not limited to, at least a 1% increase, at least a 3% increase, at least a 5% increase, at least a 10% increase, at least a 20% increase, at least a 30%, at least a 50%, at least a 70%, at least a 100% or a greater Page 48 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) increase in yield compared to a plant not expressing the pesticidal sequence. In specific methods, plant yield is increased as a result of improved pest resistance of a plant expressing a pesticidal protein disclosed herein. Expression of the pesticidal protein results in a reduced ability of a pest to infest or feed. Further provided is a method for protecting a plant from an insect pest, comprising expressing in a plant or cell thereof a nucleotide sequence that encodes a pesticidal polypeptide, wherein the nucleotide sequence comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; or, (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18. The plants can also be treated with one or more chemical compositions, including one or more herbicide, insecticides, or fungicides. In certain embodiments the polynucleotides of the present invention, including the sequences set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, and 17, can be stacked with any combination of polynucleotide sequences of interest in order to create plants with a desired trait. A trait, as used herein, refers to the phenotype derived from a particular sequence or groups of sequences. For example, the polynucleotides of the present invention may be stacked with any other polynucleotides encoding polypeptides having pesticidal and / or insecticidal activity, such as Bacillus thuringiensis toxic proteins (described in U.S. Patent Nos.5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; and Geiser et al. (1986) Gene 48:109), lectins (Van Damme et al. (1994) Plant Mol. Biol. 24:825, pentin (described in U.S. Patent No.5,981,722), and the like. The combinations generated can also include multiple copies of any one of the polynucleotides provided herein. The polynucleotides of the present invention can also be stacked with any other gene or combination of genes to produce plants with a variety of desired trait combinations including, but not limited to, traits desirable for animal feed such as high oil genes (e.g., U.S. Patent No.6,232,529); balanced amino acids (e.g., hordothionins (U.S. Patent Nos.5,990,389; 5,885,801; 5,885,802; and 5,703,409); barley high lysine (Williamson et al. (1987) Eur. J. Biochem.165:99-106; and WO 98 / 20122) and high Page 49 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) methionine proteins (Pedersen et al. (1986) J. Biol. Chem.261:6279; Kirihara et al. (1988) Gene 71:359; and Musumura et al. (1989) Plant Mol. Biol.12:123)); increased digestibility (e.g., modified storage proteins (U.S. Application Serial No.10 / 053,410, filed November 7, 2001); and thioredoxins (U.S. Application Serial No.10 / 005,429, filed December 3, 2001)); the disclosures of which are herein incorporated by reference. The polynucleotides of the present invention can also be stacked with traits desirable for disease or herbicide resistance (e.g., fumonisin detoxification genes (U.S. Patent No.5,792,931); avirulence and disease resistance genes (Jones et al. (1994) Science 266:789; Martin et al. (1993) Science 262:1432; Mindrinos et al. (1994) Cell 78:1089); acetolactate synthase (ALS) mutants that lead to herbicide resistance such as the S4 and / or Hra mutations; inhibitors of glutamine synthase such as phosphinothricin or basta (e.g., bar gene); and glyphosate resistance (EPSPS gene)); and traits desirable for processing or process products such as high oil (e.g., U.S. Patent No.6,232,529 ); modified oils (e.g., fatty acid desaturase genes (U.S. Patent No.5,952,544; WO 94 / 11516)); modified starches (e.g., ADPG pyrophosphorylases (AGPase), starch synthases (SS), starch branching enzymes (SBE), and starch debranching enzymes (SDBE)); and polymers or bioplastics (e.g., U.S. Patent No.5.602,321; beta-ketothiolase, polyhydroxybutyrate synthase, and acetoacetyl-CoA reductase (Schubert et al. (1988) J. Bacteriol.170:5837-5847) facilitate expression of polyhydroxyalkanoates (PHAs)); the disclosures of which are herein incorporated by reference. One could also combine the polynucleotides of the present invention with polynucleotides providing agronomic traits such as male sterility (e.g., see U.S. Patent No.5.583,210), stalk strength, flowering time, or transformation technology traits such as cell cycle regulation or gene targeting (e.g., WO 99 / 61619, WO 00 / 17364, and WO 99 / 25821); the disclosures of which are herein incorporated by reference. These stacked combinations can be created by any method including, but not limited to, cross-breeding plants by any conventional or TopCross methodology, or genetic transformation. If the sequences are stacked by genetically transforming the plants, the polynucleotide sequences of interest can be combined at any time and in any order. For example, a transgenic plant comprising one or more desired traits can be used as the target to introduce further traits by subsequent transformation. The traits can be Page 50 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) introduced simultaneously in a co-transformation protocol with the polynucleotides of interest provided by any combination of transformation cassettes. For example, if two sequences will be introduced, the two sequences can be contained in separate transformation cassettes (trans) or contained on the same transformation cassette (cis). Expression of the sequences can be driven by the same promoter or by different promoters. In certain cases, it may be desirable to introduce a transformation cassette that will suppress the expression of the polynucleotide of interest. This may be combined with any combination of other suppression cassettes or overexpression cassettes to generate the desired combination of traits in the plant. It is further recognized that polynucleotide sequences can be stacked at a desired genomic location using a site- specific recombination system. See, for example, WO99 / 25821, WO99 / 25854, WO99 / 25840, WO99 / 25855, and WO99 / 25853, all of which are herein incorporated by reference. Non-limiting embodiments include: 1. A polypeptide comprising: (a) an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 2. The polypeptide of embodiment 1, further comprising heterologous amino acid sequences. 3. The polypeptide of embodiment 1 or 2, wherein the polypeptide is an isolated polypeptide. Page 51 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 4. The polypeptide of embodiment 1 or 2, wherein the polypeptide is a recombinant polypeptide. 5. A nucleic acid molecule comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. 6. The nucleic acid molecule of embodiment 5, wherein said nucleic acid molecule is not a naturally occurring sequence encoding said polypeptide. 7. The nucleic acid molecule of embodiment 5, wherein the nucleic acid molecule is an isolated nucleic acid molecule. Page 52 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 8. The nucleic acid molecule of embodiment 5, wherein the nucleic acid molecule is a recombinant nucleic acid molecule. 9. The nucleic acid molecule of embodiment 5, wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant. 10. A host cell comprising a nucleic acid molecule comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. 11. The host cell of embodiment 10, wherein the host cell comprises (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10 and a nucleotide sequence encoding a Page 53 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 12. The host cell of embodiment 10 or 11, wherein said host cell is a bacterial host cell or a plant cell. 13. A DNA construct comprising a heterologous promoter operably linked to a nucleic acid sequence comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in Page 54 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. 14. The DNA construct of embodiment 13, wherein the DNA construct comprises (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 15. The DNA construct of embodiment 13 or 14, wherein the promoter drives expression in a plant cell. Page 55 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 16. The DNA construct of any one of embodiments 13-15, wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant. 17. A vector comprising the DNA construct of any one of embodiments 13-16. 18. A host cell comprising the DNA construct of any one of embodiments 13-16 or the vector of embodiment 17. 19. The host cell of embodiment 18, wherein the host cell is a plant cell. 20. A transgenic plant comprising the host cell of embodiment 19. 21. The DNA construct of embodiment 13 or 14, wherein the promoter drives expression in a bacterial cell. 22. A vector comprising the DNA construct of embodiment 21. 23. A host cell comprising the DNA construct of embodiment 21 or the vector of embodiment 22. 24. A formulation comprising a polypeptide, wherein the polypeptide comprises: (a) an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 25. A formulation comprising: (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a polypeptide comprising an amino Page 56 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 26. The formulation of embodiment 24 or 25, wherein said formulation is selected from the group consisting of a powder, dust, pellet, granule, a wettable granule, a disbursable flowable, a wettable powder, spray, emulsion, colloid, an aqueous solution, an oil-based solution, or a liquid. 27. The formulation of any one of embodiments 24-26, wherein said formulation comprises from about 1% to about 99% by weight of said polypeptide. 28. A method for controlling a pest population comprising contacting said population with a pesticidal-effective amount of the formulation of any one of embodiments 24-27. 29. A method for killing a pest population comprising contacting said population with a pesticidal-effective amount of the formulation of any one of embodiments 24-27. 30. A method for producing a polypeptide with pesticidal activity, comprising culturing the host cell of any one of embodiments 10-12, 18, or 19 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed. 31. A plant having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in Page 57 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. 32. A plant having stably incorporated into its genome a DNA construct comprising: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence Page 58 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 33. A transgenic seed of the plant of embodiment 31 or 32, wherein said seed has stably incorporated into its genome the DNA construct. 34. A method for controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof a nucleic acid molecule, wherein said nucleic acid molecule comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. Page 59 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 35. A method for controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 36. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in Page 60 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity. 37. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in Page 61 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. 38. The method of any one of embodiments 34-37, wherein said plant produces a pesticidal polypeptide having pesticidal activity against a lepidopteran pest, a hemipteran pest, or a coleopteran pest. 39. The method of embodiment 38, wherein said lepidopteran pest, hemipteran pest, or said coleopteran pest is resistant to one or more strains of Bacillus thuringiensis or one or more toxin proteins produced by one or more strains of Bacillus thuringiensis. 40. The method of embodiment 31, wherein said lepidopteran pest, hemipteran pest, or said coleopteran pest is resistant to any one of Cry34 / 35 and Cry3Bb. 41. The method of any one of embodiments 34-40, wherein the plant is a monocot. 42. The method of any one of embodiments 34-40, wherein the plant is a dicot. 43. The method of embodiment 41, wherein the plant is corn, sorghum, wheat, rice, sugarcane, barley, oats, rye, millet, coconut, pineapple or banana. 44. The method of embodiment 42, wherein the plant is sunflower, tomato, crucifers, peppers, potato, cotton, soybean, sugarbeet, tobacco, oilseed rape, sweet potato, alfalfa, safflower, peanuts, cassava, coffee, cocoa, cucumber, lettuce, olive, peas, or tea. 45. A method of obtaining a polynucleotide that encodes an improved polypeptide having pesticidal activity, wherein the improved polypeptide has at least one improved property over SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, said method comprising: (a) recombining a plurality of parental polynucleotides comprising any of SEQ ID NOs: 1, 3, 5, and 7 or an active variant or fragment thereof to produce a library of recombinant polynucleotides encoding recombinant pesticidal polypeptides; (b) screening the library to identify a recombinant polynucleotide that encodes an improved recombinant pesticidal polypeptide that has an enhanced property improved over the parental polynucleotide; Page 62 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) (c) recovering the recombinant polynucleotide that encodes the improved recombinant pesticidal polypeptide identified in (b); and, (d) repeating steps (a), (b) and (c) using the recombinant polynucleotide recovered in step (c) as one of the plurality of parental polynucleotides in repeated step (a). 46. The method of embodiment 38, wherein the pest is Colorado potato beetle (CPB). 47. The method of embodiment 38, wherein the pest is western corn rootworm (WCRW). The following examples are offered by way of illustration and not by way of limitation. EXPERIMENTAL Example 1: Discovery of novel Ply genes by sequencing and DNA analysis Mushroom samples were collected and stored in dry ice for transport before storage at -80°C to preserve DNA, RNA and proteins. DNA was extracted from ground sample using a MoBIO PowerSoil DNA extraction kit (Qiagen). ITS and18S rDNA sequencing was performed using the extracted DNA and the primers indicated in Table 3. Sequencing results were searched in the non-redundant DNA database provided by NCBI and were identified to be derived from the mushroom to be Laetiporus persicinus, (RG12). Table 3. Sequencing primers SEQ ID NO: Sequence (5’-3’) A C Page 63 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Protein was also extracted from the ground mushroom sample by resuspending the ground tissue in protein extraction buffer (100mM Tris, 150mM KCl, 2.5mM EDTA, 1.5% PVP, pH8 + "Complete, EDTA-free" protease inhibitor tablets (Roche, Indianapolis, Indiana)) at a ratio of approximately 4ml of buffer per gram of tissue. Solid material was then separated from the solution by centrifugation; additionally, the collected supernatant was passed through Miracloth (Millipore Sigma). The retained pellet was resuspended once more with half the volume of protein extraction buffer and the above steps repeated to extract any remaining protein from the tissue. Supernatants were then combined and the pellet was discarded. The samples were assayed for insecticidal activity against a panel of insects from three different orders listed in Table 4. These bioassays were performed using diet overlay methods and RG12 showed 100% mortality against WCRW. Table 4. Protein Activity from RG12 FAW CEW WCRW The collected supernatant (the crude extract) was then desalted with a KTA Pure (GE Healthcare Life Sciences) fast protein liquid chromatography (FPLC) system. The desalted fraction was subjected to a + / - heat treatment (95° C, 30 minutes) to determine if any observable activity is proteinaceous. Novel Protein Discovery from RG12 For protein purification, extract of RG12 was generated as described above and the supernatant desalted into 20 mM Tris, pH 8. An aliquot of this sample was saved for in vitro bioassay testing. A fraction of the desalted crude extract was heated for 30 minutes at 95°C and assayed to determine if observed activity was proteinaceous. The desalted crude extract was fractionated by anion exchange chromatography using a 5 ml Q FF column (GE Healthcare Life Sciences). The Q flow through (unbound) fraction was discarded while the bound proteins were eluted from the column with 20mM Tris + 0.5M NaCl, pH8. This eluate was desalted again using the HiPrep 26 / 10 desalting column into Page 64 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) 20mM Tris, pH8. Samples of the desalted crude extract, the heated, desalted crude extract and anion exchange bound proteins were tested in vitro against target pests. Activity data is shown in Table 7 below. Bound proteins from the anion exchange fractionation step above were subsequently desalted into 50mM Na-Formate, pH4 and taken to a 1ml CaptoS column (GE Healthcare Life Sciences) and eluted with a step gradient of 50mM Na-Formate + 0.3M NaCl, pH4. The CaptoS fractions were concentrated and washed with 20mM Tris, pH8 prior to submitting to bioassay where all activity was found in the CaptoS bound (no activity in unbound material. The CaptoS Eluate was concentrated (10kDa MWCO), and loaded on a 2x S200 column (2 columns in tandem, GE Life Sciences) pre-equilibrated with 20mM Tris, pH8 + 150mM NaCl and ran over 1.5CV using an isocratic gradient. 1ml fractions were collected from the S200 run, where fractions corresponding to 31- 37ml were collected and pooled for the next step. The S200 fraction pool was desalted back into 50mM Na-Formate, pH4 and ran on a 1ml Mono S (5x50, GE Life Sciences) column where it was ran over a 30CV gradient, utilizing 0-0.3M NaCl in 50mM Na- Formate, pH4. Each Mono S fraction was concentrated with a 10kDa MWCO unit which included a wash step with 20mM Tris, pH8. The washed material was then tested at a 5x and 1x concentration relative to the collected fractions. Peptides from proteins for mass spectrometry identification were obtained and subjected to nano-liquid chromatography / electrospray tandem mass spectrometry (nano- LC / ESI-MS / MS) on a Thermo Q Exactive™ Orbitrap™ mass spectrometer (Thermo Fisher Scientific®, Waltham, MA) interfaced with an Eksigent™ NanoLC™ Ultra 1-D Plus nano-lc system (AB Sciex™, Framingham, MA). Protein identification was done by database searches using Mascot® (Matrix Science, London UK). The searches were conducted against transcriptome data carried out on the source organism, RG12. Sequenced peptides gave a match to transcripts corresponding to APG75914 and APG54101. A summary of the sequences is set forth in Table 1. PlyA sequences of SEQ ID NOs: 2 and 10 were aligned and a consensus sequence is provided in FIG.1. PlyB sequences of SEQ ID NOs: 14 and 18 were aligned and a consensus sequence is provided in FIG.2. Alignment of all the PlyA sequences is shown in FIG.3 and alignment of all Page 65 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) the PlyB sequences is shown in FIG.4. Purified proteins were assessed in insect diet bioassay studies to identify active proteins. Example 2. Heterologous Expression in E. Coli Each open reading frame set forth in Table 9 is cloned into an E. coli expression vector containing a maltose binding protein (pMBP). The expression vector is transformed into BL21*RIPL. An LB culture supplemented with carbenicillin is inoculated with a single colony and grown overnight at 37ºC using 0.5% of the overnight culture, a fresh culture is inoculated and grown to logarithmic phase at 37ºC. The culture is induced using 250 mM IPTG for 18 hours at 16ºC. The cells are pelleted and resuspended in 10mM Tris pH7.4 and 150 mM NaCl supplemented with protease inhibitors. The protein expression is evaluated by SDS-PAGE. Example 3. Pesticidal Activity against Coleoptera and Lepidoptera  Methods Protein Expression: Each sequence set forth in Table 9 was expressed in E. coli as described in Example 2. 400 mL of LB was inoculated and grown to an OD600 of 0.6. The culture was induced with 0.25mM IPTG overnight at 16ºC. The cells were spun down and the cell pellet was resuspended in 5 mL of buffer. The resuspension was sonicated for 2 min on ice. Bioassay: Velvetbean Caterpillar (VBC), Fall armyworm (FAW), corn earworm (CEW), European corn borer (ECB), black cutworm (BCW), and southwestern corn borer (SWCB) eggs were purchased from a commercial insectary (Benzon Research Inc., Carlisle, Pa.). The VBC, FAW, CEW, and BCW eggs were incubated to the point that eclosion would occur within 12 hrs of the assay setup. ECB and SWCB were introduced to the assay as neonate larvae. Assays were carried out in 24-well trays containing multispecies lepidopteran diet (Southland Products Inc., Lake Village, Ariz.). Samples of the sonicated lysate were applied to the surface of the diet (diet overlay) and allowed to evaporate and soak into the diet. For CEW, FAW, BCW, ECB and SWCB, a 100 μl of sonicated lysate was added to the diet surface and dried. The bioassay plates were sealed Page 66 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) with a plate sealing film vented with pin holes. The plates were incubated at 26° C. at 55% relative humidity (RH) on a 14:10 day:night cycle in a Percival for 5 days. The assays were assessed for level of mortality, growth inhibition and feeding inhibition. The assay is scored based on insect mortality and stunting. Mortality is rated as no mortality (-), some mortality (M), and high mortality (HM), while stunting is rated as no stunting (-), slight stunting (SS), and strong stunting (S). For the rootworm bioassay, the protein construct / lysate was evaluated in an insect bioassay by dispensing 60 μl volume on the top surface of diet in well / s of 24-well plate (Cellstar, 24-well, Greiner Bio One) and allowed to dry. Each well contained 500 μl diet (Marrone et al., 1985). Fifteen to twenty neonate larvae were introduced in each well using a fine tip paint brush and the plate was covered with membrane (Viewseal, Greiner Bio One). The bioassay was stored at ambient temperature and scored for mortality, and / or growth / feeding inhibition at day 4 or day 5. The insecticidal assay results are shown in Table 5. Table 5: Summary of Pesticidal Activity Against Coleopteran and Lepidopteran APG Seq ID VBC FAW CEW BCW ECB SWC SBL WCRW B APG579490 / 11 1 NT NT NT NT NT NT NT + Page 67 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Example 4. Purified Protein with Pesticidal Activity (LC50) A 6xHis construct comprising SEQ ID NO: 1, 5, 7, 9, 11, 13, 17 was produced. The construct was transformed into E. coli BL21*(DE3) for protein production. The proteins were purified using standard techniques for a HIS-tagged protein and the fractions were analyzed for purity by SDS-PAGE. The purified protein was then tested on susceptible insects as a surface treatment in a diet-based assay and the results are shown in Tables 6 and 7. If the LC50 or EC50 was not determined it is stated as ND. Table 6: Summary of Pesticidal Activity of Purified Protein WCRW %Mort (Combo)8.4 Table 7: Summary of Pesticidal Activity of Purified Protein APG Seq ID Target LC50 Organism LC50 95% Confidence Example 5: Summary of Insecticidal Activity against Field-resistant Insects To determine if the proteins have a new mode-of-action from field-evolved resistant insects, lysate was tested on field-evolved resistant insects and susceptible insects. Purpose: Determine susceptibility differences between populations of Bt toxin resistant Western corn rootworm (Diabrotica virgifera virgifera) and Bt toxin susceptible WCRW Page 68 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) to PlyA / PlyB toxin. The high dose sample of PlyA / PlyB protein elicited similar activity against Cry34 / 35 resistant WCRW (Cry34 / 35-R), Cry3Bb resistant WCRW (Cry3Bb-R) and susceptible WCRW. While a lower dose elicited similar activity against Cry3Bb-R WCRW and susceptible WCRW and activity against Cry34 / 35-R WCRW to a lesser extent still suggesting a different MOA than Cry3Bb1 and Cry34 / 35 toxins. Methods: Diet overlay bioassay was performed on Cry34 / 35-R, Cry3Bb-R and susceptible WCRW (SUS) neonate larvae to assess PlyA / PlyB protein toxicity at 5 days. Samples of whole cell E.coli expressing PlyA and / or PlyB protein, inactive protein Vip3A (negative control) and active protein Cry34 / 35 (positive control) were prepared by pelleting and resuspending the cells in different volumes of LB media. Doses of PlyA / PlyB tested were equivalent to 1 and 3 times the cell concentration of the initial bacterial growth culture. LB media included as a negative control. WCRW-MO2, a semi-solid agar based artificial diet (Huynh et al.2019), was prepared and dispensed into each well of 96-well tissue culture plates. Samples were applied to the diet and air dried in a biological safety cabinet. A single neonate larva, hatched from surface sterilized WCRW egg (Ludwick et al.2018), was placed in each well. Each sample was tested against 24 neonate larvae across 3 replicates. Adhesive membranes sealed each well and pin holes were made to allow airflow. The bioassay plates were stored in a dark environmental chamber at approximately 25°C. At 5-days, the assay was evaluated. Mortality was determined and the surviving larvae were frozen for > 24h to kill the larvae, before estimating fresh (wet) weight using a Sartorius microscale. The results for the susceptible WCRW are shown in FIG.5. pGA9885 is the identifier for the combination of APG57949.1 and APG58918.4 at a 1:3 ratio (binary toxin). FIG.5 shows the percent mortality, percent molting, and weight of the larvae. At the concentrations tested, pGA9985 killed 21-100% of the susceptible WCRW larvae. No larvae molted to 2nd instar after exposed to pGA9985 and surviving larvae had significantly lower fresh weight compared to all buffer controls. Cry34 / 35 protein at 50 µg / cm2killed 100% susceptible larvae and Cry3Bb protein at 44 and 175 µg / cm2killed 33% and 38% susceptible larvae, respectively. Page 69 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) The results for the resistant WCRW larvae are shown in FIG.6 for Cry34 / 35-R WCRW and in Table 8 for Cry3Bb-R or Cry34 / 35-R resistant WCRW. 100% of the Cry34 / 35-R (resistant) larvae were killed at the highest dose (180 ug / cm2). No larvae molted to 2nd instar at concentrations ≥ 22.5 ug / cm2. Surviving larvae after being exposed to pGA9985 had significantly lower fresh weight compared to other treatments.  Controls (Cry34 / 35 and Cry3Bb proteins and buffers) showed no mortality against Cry34 / 35-RES and no significant differences in mortality, molt, and fresh weight. Table 8; Summary of Insecticidal Activity Against WCRW Field-Resistant Insects APG Seq ID No. Target Organism Concentration Mortality APG57949.1 / 13, 9 Cry3Bb-R 180 ug / cm2 92% Example 6. Root Excision Assay with WCRW in Transformed Corn Plants Maize were transformed with APG57949.1 / APG54101.0 in combination or individually as described in Example 9. Sample roots from newly transformed corn plants were placed in a small petri dish with agar and infested with WCRW larvae. The roots Page 70 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) were scored on Day 4. The results are shown in FIG.7. Cry3Bb1 transformed corn roots were used as a positive control. Similar protection was observed from binary toxin as a combination or individually compared to Cry3Bb1 control plants and less than the negative control. Example 7. Pesticidal Activity against Hemipteran Protein Expression: Each of the sequences set forth in Table 9 is expressed in E. coli as described in Example 2. 400 mL of LB is inoculated and grown to an OD600 of 0.6. The culture is induced with 0.25mM IPTG overnight at 16ºC. The cells are spun down and the cell pellet is re-suspended in 5 mL of buffer. The resuspension is sonicated for 2 min on ice. Second instar southern green stink bug (SGSB) are obtained from a commercial insectary (Benzon Research Inc., Carlisle, PA). A 50% v / v ratio of sonicated lysate sample to 20% sucrose is employed in the bioassay. Stretched parafilm is used as a feeding membrane to expose the SGSB to the diet / sample mixture. The plates are incubated at 25ºC:21ºC, 16:8 day:night cycle at 65%RH for 5 days. Mortality is scored for each sample. Example 8. Transformation of Soybean DNA constructs comprising SEQ ID NO: 2, 4, 6, or 8, or active variants or fragments thereof operably linked to a promoter active in a plant are cloned into transformation vectors and introduced into Agrobacterium as described in US Provisional Application No.62 / 094,782, filed December 19, 2015, herein incorporated by reference in its entirety. Four days prior to inoculation, several loops of Agrobacterium are streaked to a fresh plate of YEP* medium supplemented with the appropriate antibiotics** (spectinomycin, chloramphenicol and kanamycin). Bacteria are grown for two days in the dark at 28ºC. After two days, several loops of bacteria are transferred to 3 ml of YEP liquid medium with antibiotics in a 125 ml Erlenmeyer flask. Flasks are placed on a rotary shaker at 250 RPM at 28ºC overnight. One day before inoculation, 2-3 ml of the Page 71 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) overnight culture were transferred to 125 ml of YEP with antibiotics in a 500 ml Erlenmeyer flask. Flasks are placed on a rotary shaker at 250 RPM at 28ºC overnight. Prior to inoculation, the OD of the bacterial culture is checked at OD 620. An OD of 0.8-1.0 indicates that the culture is in log phase. The culture is centrifuged at 4000 RPM for 10 minutes in Oakridge tubes. The supernatant is discarded and the pellet is re- suspended in a volume of Soybean Infection Medium (SI) to achieve the desired OD. The cultures are held with periodic mixing until needed for inoculation. Two or three days prior to inoculation, soybean seeds are surface sterilized using chlorine gas. In a fume hood, a petri dish with seeds is place in a bell jar with the lid off. 1.75 ml of 12 N HCl is slowly added to 100 ml of bleach in a 250 ml Erlenmeyer flask inside the bell jar. The lid is immediately placed on top of the bell jar. Seeds are allowed to sterilize for 14-16 hours (overnight). The top is removed from the bell jar and the lid of the petri dish is replaced. The petri dish with the surface sterilized is then opened in a laminar flow for around 30 minutes to disperse any remaining chlorine gas. Seeds are imbibed with either sterile DI water or soybean infection medium (SI) for 1-2 days. Twenty to 30 seeds are covered with liquid in a 100x25 mm petri dish and incubated in the dark at 24ºC. After imbibition, non-germinating seeds are discarded. Cotyledonary explants are processed on a sterile paper plate with sterile filter paper dampened using SI medium employing the methods of U.S. Patent 7,473,822, herein incorporated by reference. Typically, 16-20 cotyledons are inoculated per treatment. The SI medium used for holding the explants is discarded and replaced with 25 ml of Agrobacterium culture (OD 620=0.8-20). After all explants are submerged, the inoculation is carried out for 30 minutes with periodic swirling of the dish. After 30 minutes, the Agrobacterium culture is removed. Co-cultivation plates are prepared by overlaying one piece of sterile paper onto Soybean Co-cultivation Medium (SCC). Without blotting, the inoculated cotyledons are cultured adaxial side down on the filter paper. Around 20 explants can be cultured on each plate. The plates are sealed with Parafilm and cultured at 24ºC and around 120 µmoles m-2s-1 (in a Percival incubator) for 4-5 days. Page 72 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) After co-cultivation, the cotyledons are washed 3 times in 25 ml of Soybean Wash Medium with 200 mg / l of cefotaxime and timentin. The cotyledons are blotted on sterile filter paper and then transferred to Soybean Shoot Induction Medium (SSI). The nodal end of the explant is depressed slightly into the medium with distal end kept above the surface at about 45deg. No more than 10 explants are cultured on each plate. The plates are wrapped with Micropore tape and cultured in the Percival at 24ºC and around 120 µmoles m-2s-1. The explants are transferred to fresh SSI medium after 14 days. Emerging shoots from the shoot apex and cotyledonary node are discarded. Shoot induction is continued for another 14 days under the same conditions. After 4 weeks of shoot induction, the cotyledon is separated from the nodal end and a parallel cut is made underneath the area of shoot induction (shoot pad). The area of the parallel cut is placed on Soybean Shoot Elongation Medium (SSE) and the explants cultured in the Percival at 24ºC and around 120 umoles m-2s-1. This step is repeated every two weeks for up to 8 weeks as long as shoots continue to elongate. When shoots reach a length of 2-3 cm, they are transferred to Soybean Rooting Medium (SR) in a Plantcon vessel and incubated under the same conditions for 2 weeks or until roots reach a length of around 3-4 cm. After this, plants are transferred to soil. Note, all media mentioned for soybean transformation are found in Paz et al. (2010) Agrobacterium-mediated transformation of soybean and recovery of transgenic soybean plants; Plant Transformation Facility of Iowa State University, which is herein incorporated by reference in its entirety. (See, agron-www.agron.iastate.edu / ptf / protocol / Soybean.pdf.) Example 9. Transformation of Maize Maize ears are best collected 8-12 days after pollination. Embryos are isolated from the ears, and those embryos 0.8-1.5 mm in size are preferred for use in transformation. Embryos are plated scutellum side-up on a suitable incubation media, such as DN62A5S media (3.98 g / L N6 Salts; 1 mL / L (of 1000.times. Stock) N6 Vitamins; 800 mg / L L-Asparagine; 100 mg / L Myo-inositol; 1.4 g / L L-Proline; 100 mg / L Casamino acids; 50 g / L sucrose; 1 mL / L (of 1 mg / mL Stock) 2,4-D). However, media and salts other than DN62A5S are suitable and are known in the art. Embryos are Page 73 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) incubated overnight at 25ºC in the dark. However, it is not necessary per se to incubate the embryos overnight. The resulting explants are transferred to mesh squares (30-40 per plate), transferred onto osmotic media for about 30-45 minutes, then transferred to a beaming plate (see, for example, PCT Publication No. WO / 0138514 and U.S. Pat. No.5,240,842). DNA constructs designed to express the GRG proteins of the present invention in plant cells are accelerated into plant tissue using an aerosol beam accelerator, using conditions essentially as described in PCT Publication No. WO / 0138514. After beaming, embryos are incubated for about 30 min on osmotic media, and placed onto incubation media overnight at 25ºC in the dark. To avoid unduly damaging beamed explants, they are incubated for at least 24 hours prior to transfer to recovery media. Embryos are then spread onto recovery period media, for about 5 days, 25ºC in the dark, then transferred to a selection media. Explants are incubated in selection media for up to eight weeks, depending on the nature and characteristics of the particular selection utilized. After the selection period, the resulting callus is transferred to embryo maturation media, until the formation of mature somatic embryos is observed. The resulting mature somatic embryos are then placed under low light, and the process of regeneration is initiated by methods known in the art. The resulting shoots are allowed to root on rooting media, and the resulting plants are transferred to nursery pots and propagated as transgenic plants. Example 10. Pesticidal activity against Nematodes. Heterodera glycine’s (Soybean Cyst Nematode) in-vitro assay. Soybean Cyst Nematodes are dispensed into a 96 well assay plate with a total volume of 100uls and 100 J2 per well. The protein of interest as set forth in SEQ ID NO: 2, 4, 6, and / or 8 is dispensed into the wells and held at room temperature for assessment. Finally, the 96 well plate containing the SCN J2 is analyzed for motility. Data is reported as % inhibition as compared to the controls. Hits are defined as greater or equal to 70% inhibition. Page 74 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Heterodera glycine’s (Soybean Cyst Nematode) on-plant assay Soybean plants expressing SEQ ID NO: 2, 4, 6, and / or 8 are generated as described elsewhere herein. A 3-week-old soybean cutting is inoculated with 5000 SCN eggs per plant. This infection is held for 70 days and then harvested for counting of SCN cyst that has developed on the plant. Data is reported as % inhibition as compared to the controls. Hits are defined as greater or equal to 90% inhibition. Meloidogyne incognita (Root-Knot Nematode) in-vitro assay Root-Knot Nematodes are dispensed into a 96 well assay plate with a total volume of 100µls and 100 J2 per well. The protein(s) of interest is dispensed into the wells and held at room temperature for assessment. Finally, the 96 well plate containing the RKN J2 is analyzed for motility. Data is reported as % inhibition as compared to the controls. Hits are defined as greater or equal to 70% inhibition. Meloidogyne incognita (Root-Knot Nematode) on-plant assay Soybean plants expressing SEQ ID NO: 2, 4, 6, and / or 8 are generated as described elsewhere herein. A 3-week-old soybean is inoculated with 5000 RKN eggs per plant. This infection is held for 70 days and then harvested for counting of RKN eggs that have developed in the plant. Data is reported as % inhibition as compared to the controls. Hits are defined as greater or equal to 90% inhibition. Example 11. Additional Assays for Pesticidal Activity The polypeptide set forth in SEQ ID NO: 2, 4, 6, and / or 8 can be tested to act as a pesticide upon a pest in a number of ways. One such method is to perform a feeding assay. In such a feeding assay, one exposes the pest to a sample containing either compounds to be tested or control samples. Often this is performed by placing the material to be tested, or a suitable dilution of such material, onto a material that the pest will ingest, such as an artificial diet. The material to be tested may be composed of a liquid, solid, or slurry. The material to be tested may be placed upon the surface and then allowed to dry. Alternatively, the material to be tested may be mixed with a molten artificial diet, and then dispensed into the assay chamber. The assay chamber may be, for example, a cup, a dish, or a well of a microtiter plate. Page 75 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) Assays for sucking pests (for example aphids) may involve separating the test material from the insect by a partition, ideally a portion that can be pierced by the sucking mouth parts of the sucking insect, to allow ingestion of the test material. Often the test material is mixed with a feeding stimulant, such as sucrose, to promote ingestion of the test compound. Other types of assays can include microinjection of the test material into the mouth, or gut of the pest, as well as development of transgenic plants, followed by test of the ability of the pest to feed upon the transgenic plant. Plant testing may involve isolation of the plant parts normally consumed, for example, small cages attached to a leaf, or isolation of entire plants in cages containing insects. Other methods and approaches to assay pests are known in the art, and can be found, for example in Robertson and Preisler, eds. (1992) Pesticide bioassays with arthropods, CRC, Boca Raton, Fla. Alternatively, assays are commonly described in the journals Arthropod Management Tests and Journal of Economic Entomology or by discussion with members of the Entomological Society of America (ESA). SEQ ID NO: 2, 4, 6, or 8 can be expressed and employed in an assay as set forth in Examples 3 and 4, herein. Table 9. Summary of SEQ ID NOs: 1-18 APG Seq ID Sequence Source ID NO: Organism s s s s Page 76 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) APG58 3 ATGGCGTATGCTCAGTGGGTCATCATTATTATCCATAATGTG Laetiporus 918.1 GGTAGTAAAGCTGTAAAGATTGCCAACTTGAACGCCAGTTG persicinus GGGCAAGATTCTGGCGAGCGGAGATAAGGATACGGAAGTC s s s s s s s s s s Page 77 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) APG58 9 ATGGCCTACGCCCAGTGGGTGATCATCATCATCCACAATGT 91.4 GGGCTCTAAGGCCGTGAAGATCGCCAATCTGAATGCCTCTT GGGGCAAGCTGTACGCCGATGGCGATAAGGATACAGAGGT s s s s Page 78 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) GCTTTGACTTACAGTCTGTGGTTCGTGTCAAGATTCCGGGCT CAGGCAATCCACCACGCCCAATGGTCGCACTGCGTAAAGAG ATGGTACTTGTCGAAATGCAGTTCTAA s s s s Page 79 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) CCAGCCAGGTGGGATCTGCATGGCGATCAGATCGATACAGC CGTGTACATCACAAGGCCAATCACAGGCGTGCCACCAGAGG ATGAATGCTTCGATCTGCAGTCTGTGGTGAGGGTGAAGATC s s s s Page 80 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) GCACGCTGGGATCTGCATGGTGATCAGATCGATACAGCGGT CTATATCACCAGACCGATAACAGGTGTACCACCGGAGGATG AATGCTTCGATTTGCAATCAGTAGTGCGTGTAAAGATCCCC s s s s Page 81 of 97   WBD (US) 4854-4443-2791v1  Atty Docket No. G1224241100WO (0143.1) CCCCAGCCAGGTGGGATCTGCATGGCGATCAGATCGATACA GCCGTGTACATCACAAGGCCAATCACAGGCGTGCCACCAGA GGATGAATGCTTCGATCTGCAGTCTGTGGTGAGGGTGAAGA s s All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims. Page 82 of 97   WBD (US) 4854-4443-2791v1

Claims

Atty Docket No. G1224241100WO (0143.1) That which is claimed is:

1. A polypeptide comprising: (a) an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

2. The polypeptide of claim 1, further comprising heterologous amino acid sequences.

3. The polypeptide of claim 1 or 2, wherein the polypeptide is an isolated polypeptide.

4. The polypeptide of claim 1 or 2, wherein the polypeptide is a recombinant polypeptide.

5. A nucleic acid molecule comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino Page 83 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) acid sequence set forth in SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

6. The nucleic acid molecule of claim 5, wherein said nucleic acid molecule is not a naturally occurring sequence encoding said polypeptide.

7. The nucleic acid molecule of claim 5, wherein the nucleic acid molecule is an isolated nucleic acid molecule.

8. The nucleic acid molecule of claim 5, wherein the nucleic acid molecule is a recombinant nucleic acid molecule.

9. The nucleic acid molecule of claim 5, wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant. Page 84 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) 10. A host cell comprising a nucleic acid molecule comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

11. The host cell of claim 10, wherein the host cell comprises (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10 and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; Page 85 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

12. The host cell of claim 10 or 11, wherein said host cell is a bacterial host cell or a plant cell.

13. A DNA construct comprising a heterologous promoter operably linked to a nucleic acid sequence comprising: (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in Page 86 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

14. The DNA construct of claim 13, wherein the DNA construct comprises (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity. Page 87 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) 15. The DNA construct of claim 13 or 14, wherein the promoter drives expression in a plant cell.

16. The DNA construct of any one of claims 13-15, wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant.

17. A vector comprising the DNA construct of any one of claims 13-16.

18. A host cell comprising the DNA construct of any one of claims 13-16 or the vector of claim 17.

19. The host cell of claim 18, wherein the host cell is a plant cell.

20. A transgenic plant comprising the host cell of claim 19.

21. The DNA construct of claim 13 or 14, wherein the promoter drives expression in a bacterial cell.

22. A vector comprising the DNA construct of claim 21.

23. A host cell comprising the DNA construct of claim 21 or the vector of claim 22.

24. A formulation comprising a polypeptide, wherein the polypeptide comprises: (a) an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; Page 88 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) (c) an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

25. A formulation comprising: (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

26. The formulation of claim 24 or 25, wherein said formulation is selected from the group consisting of a powder, dust, pellet, granule, a wettable granule, a disbursable flowable, a wettable powder, spray, emulsion, colloid, an aqueous solution, an oil-based solution, or a liquid.

27. The formulation of any one of claims 24-26, wherein said formulation comprises from about 1% to about 99% by weight of said polypeptide. Page 89 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) 28. A method for controlling a pest population comprising contacting said population with a pesticidal-effective amount of the formulation of any one of claims 24- 27.

29. A method for killing a pest population comprising contacting said population with a pesticidal-effective amount of the formulation of any one of claims 24-27.

30. A method for producing a polypeptide with pesticidal activity, comprising culturing the host cell of any one of claims 10-12, 18, or 19 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

31. A plant having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; Page 90 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

32. A plant having stably incorporated into its genome a DNA construct comprising: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

33. A transgenic seed of the plant of claim 31 or 32, wherein said seed has stably incorporated into its genome the DNA construct. Page 91 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) 34. A method for controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof a nucleic acid molecule, wherein said nucleic acid molecule comprises (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

35. A method for controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that Page 92 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

36. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in Page 93 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity; or (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, or 17, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

37. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule, wherein said nucleic acid molecule comprises: (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, and a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18; (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity; or (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 2, 4, 6, 8, or 10, wherein the polypeptide has pesticidal activity, and a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence Page 94 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO: 12, 14, 16, or 18, wherein the polypeptide has pesticidal activity.

38. The method of any one of claims 34-37, wherein said plant produces a pesticidal polypeptide having pesticidal activity against a lepidopteran pest, a hemipteran pest, or a coleopteran pest.

39. The method of claim 38, wherein said lepidopteran pest, hemipteran pest, or said coleopteran pest is resistant to one or more strains of Bacillus thuringiensis or one or more toxin proteins produced by one or more strains of Bacillus thuringiensis.

40. The method of claim 31, wherein said lepidopteran pest, hemipteran pest, or said coleopteran pest is resistant to any one of Cry34 / 35 and Cry3Bb.

41. The method of any one of claims 34-40, wherein the plant is a monocot.

42. The method of any one of claims 34-40, wherein the plant is a dicot.

43. The method of claim 41, wherein the plant is corn, sorghum, wheat, rice, sugarcane, barley, oats, rye, millet, coconut, pineapple or banana.

44. The method of claim 42, wherein the plant is sunflower, tomato, crucifers, peppers, potato, cotton, soybean, sugarbeet, tobacco, oilseed rape, sweet potato, alfalfa, safflower, peanuts, cassava, coffee, cocoa, cucumber, lettuce, olive, peas, or tea.

45. A method of obtaining a polynucleotide that encodes an improved polypeptide having pesticidal activity, wherein the improved polypeptide has at least one improved property over SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, or 18, said method comprising: Page 95 of 97   WBD (US) 4854-4443-2791v1 Atty Docket No. G1224241100WO (0143.1) (a) recombining a plurality of parental polynucleotides comprising any of SEQ ID NOs: 1, 3, 5, and 7 or an active variant or fragment thereof to produce a library of recombinant polynucleotides encoding recombinant pesticidal polypeptides; (b) screening the library to identify a recombinant polynucleotide that encodes an improved recombinant pesticidal polypeptide that has an enhanced property improved over the parental polynucleotide; (c) recovering the recombinant polynucleotide that encodes the improved recombinant pesticidal polypeptide identified in (b); and, (d) repeating steps (a), (b) and (c) using the recombinant polynucleotide recovered in step (c) as one of the plurality of parental polynucleotides in repeated step (a).

46. The method of claim 38, wherein the pest is Colorado potato beetle (CPB).

47. The method of claim 38, wherein the pest is western corn rootworm (WCRW). Page 96 of 97   WBD (US) 4854-4443-2791v1

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