Composition containing recombinant collagen, preparation method therefor, and use thereof

Abstract

Provided are a composition containing recombinant collagen, a preparation method therefor, and use thereof. The composition containing recombinant collagen comprises, in parts by weight, 1-10 parts of recombinant collagen, 0.5-5 parts of schizophyllan, 1-8 parts of silanized hyaluronate, 1-7 parts of a pomegranate extract, 1-20 parts of a humectant, and 20-99 parts of water. Through the combined use of recombinant collagen, schizophyllan, silanized hyaluronate, the pomegranate extract, and the humectant, the five components have a synergistic effect, which can optimize the anti-wrinkle and moisturizing effects of the composition, enhance cell repair capabilities, reduce irritation, and improve comfort. The composition is particularly suitable for anti-wrinkle and moisturizing applications in the eye area.

Description

A composition containing recombinant collagen and its preparation method and application Technical Field

[0001] The present application belongs to the technical field of daily cosmetics, and specifically relates to a composition containing recombinant collagen, a preparation method thereof, and an application thereof. Background Art

[0002] As we age, the collagen content in our skin decreases, causing it to sag and wrinkle. Research shows that the skin around the eyes is particularly delicate, thin, and wrinkled, leading to the fastest evaporation of moisture. Furthermore, the skin around the eyes has fewer sweat and sebaceous glands, making it particularly susceptible to dryness and dehydration. These factors make the eyes the most vulnerable area to aging and problems. Generally, after the age of 25, the skin around the eyes begins to deteriorate, leading to dark circles, crow's feet, eye bags, granulation, and edema. Therefore, anti-wrinkle, moisturizing, and repairing the skin, especially the eye area, are crucial.

[0003] CN103961299A discloses an eye cream, the active ingredients of which are made from the following components in parts by weight: 3-5 parts camellia essential oil, 3-5 parts sage essential oil, 5-10 parts almond essential oil, 15-20 parts lemon juice, 15-20 parts carrot juice, 3-5 parts vitamin C, 5-8 parts honey, 10-15 parts glycerin, 5-8 parts tomato juice, and 10-15 parts water. The present invention also discloses a method for making the eye cream. The eye cream of the present invention is mild and non-irritating, and is a hypoallergenic eye cream that can effectively combat problems such as dryness, fine lines, and dark circles, while also increasing cell vitality, fighting inflammation, and resisting oxidation, and the preparation method is simple.

[0004] CN116421506A discloses an anti-wrinkle and firming eye cream and a preparation method thereof, the eye cream comprising the following components in parts by weight: 8-25 parts of a moisturizer, 7-16 parts of an emollient, 2-5 parts of an emulsifier, 0.4-1.2 parts of a thickener, 0.05-0.3 parts of a chelating agent, 4-15 parts of an anti-wrinkle and firming ingredient, and a solvent supplemented to 100 parts. The present invention utilizes six raw materials to form an anti-wrinkle and firming ingredient, namely, a bifid yeast fermentation product lysate, β-glucan, hydroxypropyl tetrahydropyrantriol, a combination of Centella asiatica extract / Polygonum cuspidatum root extract / Scutellaria baicalensis root extract / Tea leaf extract / Glycyrrhiza glabra extract / Matricaria flower extract / Rosemary leaf extract, a combination of Agrimonia dactylifera extract / Ampelopsis grandis leaf extract, and a combination of lactose / whey protein / lactic acid. The synergistic effect of the components can significantly reduce the skin firmness R0 value and reduce the area, number, and length of crow's feet, achieving very significant results.

[0005] However, the above-mentioned eye care products all contain certain irritating ingredients, which are irritating to the skin, especially the sensitive skin around the eyes. Therefore, there is an urgent need to provide a low-irritating product with good moisturizing, anti-wrinkle and repairing effects to meet application needs.

[0006] Summary of the Invention

[0007] The following is a summary of the subject matter described in detail herein. This summary is not intended to limit the scope of the claims.

[0008] In view of the deficiencies of the existing technology, the purpose of this application is to provide a composition containing recombinant collagen and its preparation method and application. The product components provided in this application have mild performance and excellent anti-wrinkle, repair and moisturizing effects.

[0009] To achieve this goal, this application adopts the following technical solutions:

[0010] In a first aspect, the present application provides a composition containing recombinant collagen, which comprises, by weight, 1-10 parts of recombinant collagen, 0.5-5 parts of schizophyllan, 1-8 parts of silanized hyaluronic acid ester, 1-7 parts of pomegranate extract, 1-20 parts of moisturizer and 20-99 parts of water.

[0011] The recombinant collagen described in the present application has the effects of moisturizing, replenishing skin collagen, promoting cell proliferation, anti-oxidation, anti-aging, etc., and has good tissue compatibility, skin permeability and safety; the schizophyllan polysaccharide is a natural β-D-glucan produced from schizophyllan, composed of a (1-3)-linked β-D-pyranose glucose residue backbone and a (1-6)-linked pyranose glucose residue side chain, and has the effects of cell repair and moisturizing; the silanol hyaluronate complex (silanol hyaluronate The invention discloses a hyaluronic acid compound (SHAC) which is a hyaluronic acid derivative that can promote cell proliferation and enhance the ability of cells to repair oxidative damage. The pomegranate extract has excellent effects on delaying skin aging, moisturizing, and anti-wrinkle, can reduce the damage of free radicals to the skin, and effectively reduces the formation of eye wrinkles by stimulating collagen-producing cells. The moisturizer can delay water loss, increase water penetration into the dermis and epidermis, provide temporary protection for the skin, reduce damage, and promote the repair process. The combination of the five has a synergistic effect, which can enhance the anti-wrinkle and moisturizing effect of the composition, promote the cell repair ability, have low irritation, and provide a strong sense of comfort, making it particularly suitable for anti-wrinkle and moisturizing of the eyes.

[0012] The amount of the recombinant collagen in the composition containing recombinant collagen of the present application can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0013] The amount of Schizophyllan added can be 0.6 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.;

[0014] The amount of the silanized glass ester added can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts or 7.5 parts, etc.;

[0015] The added amount of the pomegranate extract can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts or 6.5 parts, etc.;

[0016] The amount of water added can be 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts or 95 parts, etc.

[0017] In one embodiment, the composition containing recombinant collagen comprises, by weight, 1-5 parts of recombinant collagen, 0.6-2 parts of schizophyllan, 3-6 parts of silanized hyaluronic acid ester, 1-5 parts of pomegranate extract, 1-10 parts of moisturizer and 20-99 parts of water.

[0018] In one embodiment, the composition containing recombinant collagen comprises, by weight, 2-4 parts of recombinant collagen, 0.8-1.5 parts of octenylsuccinic anhydride-modified Schizophyllan, 3-5 parts of silanized hyaluronic acid ester, 2-4 parts of pomegranate extract, 2-8 parts of moisturizer and 20-99 parts of water.

[0019] In one embodiment, the recombinant collagen includes any one of type I recombinant collagen, type II recombinant collagen and type III recombinant collagen.

[0020] In one embodiment, the amino acid sequence of the recombinant collagen includes amino acids 950-1075 of type I collagen, and amino acids 594-729 and 1193-1199 of type III collagen.

[0021] In one embodiment, the Schizophyllan is octenylsuccinic anhydride-modified Schizophyllan.

[0022] As an optional technical solution of the present application, after the Schizophyllan is modified with octenylsuccinic anhydride, not only its stability is improved, but also its compatibility with other components is better.

[0023] In one embodiment, the moisturizing agent includes any one of glycerin, butylene glycol, or sodium hyaluronate, or a combination of at least two thereof.

[0024] In one embodiment, the humectant is a combination of glycerin, butylene glycol and sodium hyaluronate.

[0025] In one embodiment, the mass ratio of glycerol, butylene glycol and sodium hyaluronate is (1-3):(1-2):(0.1-1).

[0026] Wherein "1-3" can be 1.2, 1.4, 1.5, 1.7, 1.9, 2, 2.2, 2.4, 2.5, 2.7 or 2.9, etc.;

[0027] "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0028] “0.1-1” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 or 0.9, etc.

[0029] In one embodiment, the composition containing recombinant collagen further comprises any one of an emulsifier, a stabilizer or a thickener, or a combination of at least two thereof.

[0030] In one embodiment, the recombinant collagen-containing composition further comprises, by weight, 1-5 parts of an emulsifier, 0.1-2 parts of a stabilizer, or 0.1-1 parts of a thickener, or any one or a combination of at least two of them.

[0031] The amount of the emulsifier added can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.;

[0032] The amount of the stabilizer added can be 0.2 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, 1.5 parts, 1.7 parts or 1.9 parts, etc.;

[0033] The added amount of the thickener can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts or 0.9 parts, etc.

[0034] In one embodiment, the emulsifier includes any one or a combination of at least two of alkyl glycoside, hydrogenated lecithin, sodium alginate, lanolin or sucrose ester.

[0035] In one embodiment, the emulsifier is a combination of alkyl glycoside and hydrogenated lecithin.

[0036] In one embodiment, the mass ratio of the alkyl glycoside to hydrogenated lecithin is 1:(2-3), for example, 1:2.1, 1:2.2, 1:2.3, 1:2.4, 1:2.5, 1:2.6, 1:2.7, 1:2.8 or 1:2.9.

[0037] In one embodiment, the stabilizer comprises EDTA-2Na and / or sodium oleate.

[0038] In one embodiment, the thickener includes any one of xanthan gum, carbomer, or sodium acrylate copolymer, or a combination of at least two thereof.

[0039] Any specific point value within the above numerical range can be selected and will not be described in detail here.

[0040] In a second aspect, the present application provides a method for preparing a composition containing recombinant collagen as described in the first aspect, the method comprising mixing recombinant collagen, schizophyllan, silanized hyaluronic acid ester, pomegranate extract, and a moisturizer in water, and homogenizing to obtain the composition containing recombinant collagen.

[0041] In one embodiment, the mixing further comprises adding any one or a combination of at least two of an emulsifier, a stabilizer or a thickener.

[0042] In a third aspect, the present application provides a use of the composition containing recombinant collagen as described in the first aspect in a skin care product;

[0043] In one embodiment, the added amount of the composition containing recombinant collagen accounts for 0.1-20% of the total mass of the skin care product, for example, it can be 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18% or 19%, etc.

[0044] In one embodiment, the skin care product is an eye skin care product.

[0045] Compared with the prior art, this application has the following beneficial effects:

[0046] (1) The composition of the present application is a combination of recombinant collagen, schizophyllan, silanized hyaluronic acid ester, pomegranate extract and moisturizer. The five ingredients have a synergistic effect, which can enhance the anti-wrinkle and moisturizing effect of the composition, promote cell repair ability, have low irritation, and provide a strong sense of comfort. It is particularly suitable for anti-wrinkle and moisturizing of the eyes.

[0047] (2) The preparation method of the composition containing recombinant collagen provided in the present application has simple operation steps, low cost, and is easy to industrialize and mass-produce.

[0048] Still other aspects will become apparent upon reading and understanding the detailed description. DETAILED DESCRIPTION

[0049] In order to further illustrate the technical means and effects adopted by this application, the technical solution of this application is further explained below in combination with the preferred embodiments of this application, but this application is not limited to the scope of the embodiments.

[0050] The weight parts involved in the following examples and comparative examples are all calculated based on the actual effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH regulators, antibacterial agents, antioxidants or impurities within the permitted range.

[0051] Some of the materials and brand information involved in the examples and comparative examples of this application are as follows:

[0052] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0053] Preparation Example 1

[0054] This preparation example provides an octenylsuccinic anhydride-modified Schizophyllan, and the preparation method thereof is as follows:

[0055] (1) dissolving schizophyllan in water at a mass ratio of schizophyllan to water of 1:40, heating in a water bath at 80° C. for 2 h until the schizophyllan is completely dissolved, and hydrating at 4° C. for 12 h to obtain a schizophyllan solution;

[0056] (2) heating the schizophyllan solution to 40° C., adjusting the pH to 9, adding octenylsuccinic anhydride under continuous stirring and heating conditions, wherein the mass ratio of the schizophyllan to octenylsuccinic anhydride is 1:0.5, reacting for 3 hours, continuously measuring the pH value of the reaction system and adding 3% NaOH solution to maintain the pH value of the reaction system at 8.0-9.0, and when the pH change value is ≤1 within 20 minutes, adding 1 mol / L HCl solution to adjust the pH value of the reaction system to 6.5, stopping the reaction, and obtaining a modified schizophyllan solution;

[0057] (3) The modified Schizophyllan solution was dialyzed for 60 hours, the dialysis bag had a cutoff weight average molecular weight of 30,000, and freeze-dried to obtain the octenylsuccinic anhydride modified Schizophyllan.

[0058] Example 1

[0059] This embodiment provides a composition containing recombinant collagen, which is composed of the following components in parts by weight: 3 parts of recombinant collagen, 1 part of Schizophyllan from Preparation Example 1, 5 parts of silanized hyaluronic acid ester, 3 parts of pomegranate extract, 5 parts of moisturizer, 3 parts of emulsifier, 1 part of stabilizer, 0.5 parts of thickener, and water added to 100 parts.

[0060] The moisturizing agent is a combination of glycerin, butylene glycol and sodium hyaluronate in a mass ratio of 4:3:1;

[0061] The emulsifier is alkyl glycoside and hydrogenated lecithin in a mass ratio of 1:2.5;

[0062] The preparation method of the composition containing recombinant collagen is as follows:

[0063] The recombinant collagen, schizophyllan, silanized hyaluronic acid ester, pomegranate extract, moisturizer, emulsifier, thickener and stabilizer are mixed in water and homogenized to obtain the recombinant collagen-containing composition.

[0064] Example 2

[0065] This embodiment provides a composition containing recombinant collagen, which is composed of the following components in parts by weight: 2 parts of recombinant collagen, 2 parts of Schizophyllan from Preparation Example 1, 3 parts of silanized hyaluronic acid ester, 5 parts of pomegranate extract, 3 parts of moisturizer, 5 parts of emulsifier, 0.5 parts of stabilizer, 0.3 parts of thickener, and water added to 100 parts.

[0066] The moisturizing agent is a combination of glycerin, butylene glycol and sodium hyaluronate in a mass ratio of 3:1:1;

[0067] The emulsifier is alkyl glycoside and hydrogenated lecithin in a mass ratio of 1:2.

[0068] The preparation method of the composition containing recombinant collagen is as described in Example 1.

[0069] Example 3

[0070] This embodiment provides a composition containing recombinant collagen, which is composed of the following components in parts by weight: 5 parts of recombinant collagen, 0.6 parts of Schizophyllan from Preparation Example 1, 6 parts of silanized hyaluronic acid ester, 1 part of pomegranate extract, 10 parts of moisturizer, 1 part of emulsifier, 1.5 parts of stabilizer, 0.4 parts of thickener, and water added to 100 parts.

[0071] The moisturizing agent is a combination of glycerin, butylene glycol and sodium hyaluronate in a mass ratio of 1:2:0.3;

[0072] The emulsifier is alkyl glycoside and hydrogenated lecithin in a mass ratio of 1:3.

[0073] The preparation method of the composition containing recombinant collagen is as described in Example 1.

[0074] Example 4

[0075] This embodiment provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and that in Example 1 is that the emulsifier is only alkyl glycoside, and the amount of emulsifier added remains unchanged. The remaining components and amounts added are consistent with those in Example 1. The preparation method is similar to that in Example 1.

[0076] Example 5

[0077] This embodiment provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and that in Example 1 is that the emulsifier is hydrogenated lecithin only, and the amount of the emulsifier added remains unchanged. The remaining components and amounts added are consistent with those in Example 1. The preparation method is similar to that in Example 1.

[0078] Example 6

[0079] This embodiment provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and Example 1 is that the moisturizer is a combination of glycerol and butylene glycol in a ratio of 4:3, and the amount of moisturizer added remains unchanged. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0080] Example 7

[0081] This embodiment provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and Example 1 is that the moisturizer is a combination of glycerol and sodium hyaluronate in a ratio of 4:1, and the amount of moisturizer added remains unchanged. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0082] Example 8

[0083] This embodiment provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and Example 1 is that the moisturizer is a combination of butylene glycol and sodium hyaluronate in a ratio of 3:1, and the amount of moisturizer added remains unchanged. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0084] Example 9

[0085] This example provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and Example 1 is that an equal amount of unmodified Schizophyllan is used to replace the octenylsuccinic anhydride-modified Schizophyllan of Preparation Example 1. The remaining components and addition amounts are consistent with those of Example 1. The preparation method is similar to that of Example 1.

[0086] Example 10

[0087] This example provides a composition containing recombinant collagen. The difference between the composition containing recombinant collagen and Example 1 is that an equal amount of chitosan is used to replace the octenylsuccinic anhydride-modified Schizophyllan in Preparation Example 1. The remaining components and addition amounts are consistent with those in Example 1. The preparation method is similar to that in Example 1.

[0088] Comparative Example 1

[0089] This comparative example provides a composition containing recombinant collagen. The only difference between the composition containing recombinant collagen and Example 1 is that the components do not include schizophyllan, and the reduced amount is distributed among the recombinant collagen, silanized hyaluronic acid ester, pomegranate extract and moisturizer in proportion. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0090] Comparative Example 2

[0091] This comparative example provides a composition containing recombinant collagen. The only difference between the composition containing recombinant collagen and Example 1 is that the component does not include silanized hyaluronic acid ester, and the reduced amount is distributed to the recombinant collagen, schizophyllan, pomegranate extract and moisturizer in proportion. The remaining components and added amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0092] Comparative Example 3

[0093] This comparative example provides a composition containing recombinant collagen. The only difference between the composition containing recombinant collagen and Example 1 is that the component does not include pomegranate extract, and the reduced amount is distributed to the recombinant collagen, schizophyllan, silanized hyaluronic acid ester and moisturizer in proportion. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0094] Comparative Example 4

[0095] This comparative example provides a composition containing recombinant collagen. The only difference between the composition containing recombinant collagen and Example 1 is that the components do not include a moisturizer, and the reduced amount thereof is distributed among the recombinant collagen, schizophyllan, silanized hyaluronic acid ester and pomegranate extract in proportion. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0096] Comparative Example 5

[0097] This comparative example provides a composition with anti-wrinkle efficacy. The only difference between the composition and Example 1 is that the composition does not include recombinant collagen, and its reduced amount is distributed among the schizophyllan, silanized hyaluronic acid ester, pomegranate extract and moisturizer in proportion. The remaining components and addition amounts are consistent with those in Example 1. The preparation method refers to Example 1.

[0098] Test Example 1 Skin Anti-wrinkle Test

[0099] The anti-wrinkle performance of the samples prepared in Examples 1-10 and Comparative Examples 1-5 was evaluated:

[0100] Test instruments: Canfield Visia7 facial image analyzer (USA), Miravex Antera-3D skin imaging analyzer (Irish).

[0101] A total of 45 subjects, aged between 30 and 60 (15 males and 30 females), were divided into 15 groups, each consisting of one male and two females. The subjects were tested with the compositions prepared in Examples 1-10 and Comparative Examples 1-5. The subjects' faces were first rinsed with clean water and then blotted dry with a non-chip absorbent tissue. The subjects then sat quietly in a standard environment (temperature 20°C-22°C, humidity 40%-60%) for 30 minutes.

[0102] Test Method: Before using the test samples, the percentage of facial wrinkle area on the subjects was measured as the initial value, recorded as T0. The subjects used the compositions prepared in Examples 1-10 and Comparative Examples 1-5, and after sitting for 15 and 30 minutes, the percentage of facial wrinkle area on the subjects was measured, recorded as T15 and T30. The test results are shown in Table 1:

[0103] The formula for calculating the rate of change is:

[0104] T15 change rate = (T15-T0) / T0×100%

[0105] T30 change rate = (T30-T0) / T0×100%

[0106] Table 1

[0107] As shown in Table 1, within 30 minutes after using the different compositions, the wrinkle area percentage decreased to varying degrees, with Examples 1-3 showing better results than the others. A comparison of Example 1 with Comparative Examples 1-5 shows that the effects were poor when any one of the recombinant collagen, Schizophyllan, silanized hyaluronic acid ester, pomegranate extract, and moisturizer was missing, indicating that the recombinant collagen, Schizophyllan, silanized hyaluronic acid ester, pomegranate extract, and moisturizer exhibit a synergistic anti-wrinkle effect.

[0108] Test Example 2: Moisturizing Performance Evaluation

[0109] One hundred volunteers aged 18-40 with healthy skin, half male and half female, were randomly divided into 15 groups. The volunteers were treated with the compositions provided in Examples 1-10 and Comparative Examples 1-5. After cleansing their arms, the volunteers evenly applied 2 mg / cm2 of the composition to a 2 cm diameter test area on the inside of their arms. The application was repeated once daily for 30 consecutive days. The skin moisture content of the volunteers was measured before and after application using a skin tester (MC750, CK, Germany).

[0110] The skin moisture content of each subject was tested before use and after 30 days of continuous use. Each data was tested three times, and the average value of each group was calculated. The skin moisture content change rate was calculated according to the following formula:

[0111] Skin moisture content change rate = (skin moisture content after 30 days of use - skin moisture content before use) / skin moisture content before use × 100%; the test results are shown in Table 2:

[0112] Table 2

[0113] It can be seen from the data in Table 2 that: compared with the comparative example, the composition involved in the present application has a stronger moisturizing effect; the recombinant collagen, schizophyllan, silanized hyaluronic acid ester, pomegranate extract and moisturizer in the composition have a certain synergistic effect on the above-mentioned effects, and the combination of the five is indispensable; and the combination of moisturizers also further significantly affects the moisturizing effect of the product.

[0114] Test case 3 repair performance test:

[0115] Human skin fibroblasts (HFF-1) grown to 70%-80% of the culture dish area were obtained, digested with 0.25% trypsin, and cultured with complete culture medium to a cell density of 1×10 5 Cells were suspended at a concentration of 100 μL / mL. 100 μL of the cell suspension was inoculated into a 96-well culture plate and cultured in a 37°C, 5% CO2 saturated humidity incubator. After 24 hours of cell culture, the complete culture medium was aspirated.

[0116] The experimental group was a sample of 0.1 mg / mL of Examples 1-10 of the present application and Comparative Examples 1-5 diluted with high-glucose DMEM medium (4 replicates were set); the control group was an equal amount of high-glucose DMEM medium without the addition of a recombinant collagen composition to the cells, and the blank group was an equal amount of DMEM medium without cells, and continued to be cultured in an incubator at 37 ° C, 5% CO2 saturated humidity for 24 h, 48 h, 72 h and 96 h. At regular intervals, 10 μL of CCK-8 reagent was added to each group, incubated in a cell culture box for 1-4 h, and the absorbance (OD value) of each well was measured at a wavelength of 450 nm using an enzyme-linked immunosorbent assay. The relative proliferation rate (RGR) of the cells was calculated according to the following formula based on the mean absorbance of each group:

[0117] The results are shown in Table 3.

[0118] Table 3

[0119] It can be seen from the data in Table 3 that compared with the blank control group (100%), the relative cell proliferation rate of the composition of the present application at 24h, 48h, 72h and 96h of culture is greater than 100%, and the recombinant collagen, schizophyllan, silanized hyaluronic acid ester, pomegranate extract and moisturizer have a certain synergistic effect in this effect.

[0120] Test Example 5

[0121] Safety evaluation:

[0122] The specific method is as follows: 40 women aged 18-35 years with no history of allergies were selected, and each subject was given a trial of the product prepared in Examples 1-10. After cleaning the subject's back, a spot tester containing the sample was applied to the selected area on the back using non-irritating adhesive tape. After application, the tester was gently pressed with the finger to evenly adhere to the skin. This was continued for 24 hours. The subject was asked to keep the patch area dry for 24 hours and avoid strenuous exercise, scratching the patch area, and prolonged sunlight exposure. After 24 hours, the tester was removed and marked. After 30 minutes, once the indentation disappeared, the tester was evaluated under sufficient lighting.

[0123] The grading standards for adverse skin reactions are shown in Table 4:

[0124] Table 4

[0125] The results are shown in Table 5:

[0126] Table 5

[0127] It can be seen from the data in Table 5 that all the subjects showed negative reactions, which indicates that the composition described in the present application has no adverse reactions to the human body and is highly safe.

[0128] The applicant declares that this application uses the above-mentioned embodiments to illustrate a composition containing recombinant collagen, its preparation method, and application, but this application is not limited to the above-mentioned embodiments, that is, it does not mean that this application must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvements to this application, equivalent replacement of various raw materials of the product of this application, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the scope of protection and disclosure of this application.

[0129] The preferred embodiments of the present application are described in detail above. However, the present application is not limited to the specific details of the above embodiments. Within the technical concept of the present application, various simple modifications can be made to the technical solution of the present application, and these simple modifications all fall within the scope of protection of the present application.

[0130] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner unless there is any contradiction. In order to avoid unnecessary repetition, this application will not further describe various possible combinations.

Claims

1. A composition containing recombinant collagen, by weight, it comprises 1 - 10 parts of recombinant collagen, 0.5 - 5 parts of schizophyllan polysaccharide, 1 - 8 parts of silylated hyaluronate, 1 - 7 parts of pomegranate extract, 1 - 20 parts of humectant and 20 - 99 parts of water.

2. The composition containing recombinant collagen according to claim 1, wherein, The composition containing recombinant collagen, by weight, comprises 1 - 5 parts of recombinant collagen, 0.6 - 2 parts of schizophyllan polysaccharide, 3 - 6 parts of silylated hyaluronate, 1 - 5 parts of pomegranate extract, 1 - 10 parts of humectant and 20 - 99 parts of water; Optionally, the composition containing recombinant collagen, by weight, comprises 2 - 4 parts of recombinant collagen, 0.8 - 1.5 parts of octenyl succinic anhydride - modified schizophyllan polysaccharide, 3 - 5 parts of silylated hyaluronate, 2 - 4 parts of pomegranate extract, 2 - 8 parts of humectant and 20 - 99 parts of water.

3. The composition containing recombinant collagen according to claim 1 or 2, wherein, The schizophyllan polysaccharide is octenyl succinic anhydride - modified schizophyllan polysaccharide.

4. The composition containing recombinant collagen according to any one of claims 1-3, wherein, The humectant includes any one or a combination of at least two of glycerol, butylene glycol or sodium hyaluronate; Optionally, the humectant is a combination of glycerol, butylene glycol and sodium hyaluronate.

5. The composition containing recombinant collagen according to any one of claims 1-4, wherein, The mass ratio of glycerol, butylene glycol and sodium hyaluronate is (1 - 3):(1 - 2):(0.1 - 1).

6. The composition containing recombinant collagen according to any one of claims 1-5, wherein, The composition containing recombinant collagen further includes any one or a combination of at least two of emulsifier, stabilizer or thickener; Optionally, by weight, the composition containing recombinant collagen further includes any one or a combination of at least two of 1 - 5 parts of emulsifier, 0.1 - 2 parts of stabilizer or 0.1 - 1 part of thickener.

7. The composition containing recombinant collagen according to claim 6, wherein, The emulsifier includes any one or a combination of at least two of alkyl glycoside, hydrogenated lecithin, sodium alginate, lanolin or sucrose ester; Optionally, the emulsifier is a combination of alkyl glycoside and hydrogenated lecithin; Optionally, the mass ratio of alkyl glycoside and hydrogenated lecithin is 1:(2 - 3).

8. The composition containing recombinant collagen according to claim 6 or 7, wherein, The stabilizer includes EDTA - 2Na and / or sodium oleate; Optionally, the thickener includes any one or a combination of at least two of xanthan gum, carbomer or sodium acrylate copolymer.

9. The preparation method of the composition containing recombinant collagen according to any one of claims 1 - 8, comprising mixing recombinant collagen, schizophyllan polysaccharide, silylated hyaluronate, pomegranate extract, humectant in water, and obtaining the composition containing recombinant collagen after homogenization; Optionally, when mixing, it further includes adding any one or a combination of at least two of emulsifier, stabilizer or thickener.

10. The application of the composition containing recombinant collagen according to any one of claims 1 - 8 in skin care products; Optionally, the addition amount of the composition containing recombinant collagen accounts for 0.1 - 20% of the total mass of the skin care products; Optionally, the skin care product is an eye skin care product.

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