Calibration device and position calibration system

Abstract

The present invention relates to the technical field of position calibration, and provides a calibration device and a position calibration system, which are used for calibrating a POCT all-in-one machine equipped with a PCR module and a pipette extraction module. The calibration device comprises a calibration body configured to be compatible with the POCT all-in-one machine; a first position calibration mechanism installed on the calibration body and comprising a position adjustment component and a first measurer, wherein the position adjustment component can cooperate with the first measurer to measure the Y-direction coordinate of an amplification position when pushed by the PCR module; and a temperature probe installed on the calibration body and compatible with the PCR module so as to measure the heating temperature of the PCR module. According to the calibration device, using consumables profiling the POCT all-in-one machine in combination with the PCR module and the pipette extraction module of the POCT all-in-one machine can automatically measure the current coordinates of the amplification position and the heating temperature of the PCR module, to provide an accurate calibration basis for subsequent position and temperature calibration, and manual measurement can be avoided, while the calibration precision and the calibration efficiency are improved.

Description

Calibration devices and position calibration systems

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims the benefit of Chinese patent applications 202311871651.3, 202323663352.9, 202323663339.3 filed on December 29, 2023, and Chinese patent application 202421824591.X filed on July 30, 2024, the contents of which are incorporated herein by reference. Technical Field

[0003] The present application belongs to the technical field of position calibration, and specifically relates to a calibration device and a position calibration system. Background Art

[0004] Point-of-care testing (POCT) is a method conducted at the sampling site, using portable analytical instruments and supporting reagents to quickly obtain test results. Currently, nucleic acid testing equipment is manually calibrated before shipment using manual tooling. This is time-consuming, inconsistent, and lacks high calibration accuracy, relying heavily on the user's expertise.

[0005] Furthermore, due to sample testing requirements, there are currently a variety of different types of nucleic acid testing equipment on the market. After a client has used nucleic acid testing equipment for a period of time, due to the high degree of calibration expertise and the varying calibration requirements for different types of nucleic acid testing equipment, clients are unable to calibrate their instruments themselves, significantly impacting after-sales maintenance costs and the customer experience.

[0006] Application Contents

[0007] The main purpose of this application is to propose a calibration device to solve the technical problems of poor calibration accuracy and low efficiency of POCT all-in-one machines in the existing technology.

[0008] In order to achieve the above-mentioned objectives, the present application provides a calibration device for calibrating a POCT all-in-one machine with a PCR module and a pipetting and extraction module, wherein the calibration device includes: a calibration body, which is adapted to the POCT all-in-one machine; a first position calibration mechanism, which is installed on the calibration body and includes a position adjustment component and a first detector, and the position adjustment component can cooperate with the first detector to detect the Y-coordinate of the amplification position under the push of the PCR module; a temperature probe, which is installed on the calibration body and adapted to the PCR module to detect the heating temperature of the PCR module.

[0009] The present application also proposes a position calibration system for a nucleic acid detection device with a motion module, wherein the position calibration system includes: a calibration device, adapted to the nucleic acid detection device and used to detect the motion module; an analysis device, wherein the calibration device and the nucleic acid detection device are both communicatively connected to the analysis device; the analysis device is configured to: obtain calibration input information and device information of the nucleic acid detection device; obtain the position to be calibrated and the calibration operation instruction in combination with the calibration input information and the device information; control the motion module to drive the calibration device to move to the position to be calibrated; start calibration, and issue the calibration operation instruction to the nucleic acid detection device.

[0010] Through the above technical solution, the calibration device provided in the embodiment of the present application has the following beneficial effects:

[0011] When the calibration device calibrates the position of the POCT all-in-one machine, the calibration device and the POCT all-in-one machine can be matched and limited. The POCT all-in-one machine can drive the calibration device to move toward the PCR module until it moves to the amplification position. The PCR module can reversely push the position adjustment component, so that the position adjustment component cooperates with the first detector to automatically detect the Y coordinate of the amplification position, which can facilitate the subsequent position calibration of the amplification position. The temperature probe is adapted to the PCR module and can automatically detect the heating temperature of the PCR module, which can facilitate the subsequent temperature calibration of the PCR module. The calibration device in this application can automatically detect the current coordinates of the amplification position and the heating temperature of the PCR module by imitating the consumables of the POCT all-in-one machine and cooperating with the PCR module and pipetting extraction module of the POCT all-in-one machine itself, thereby providing an accurate calibration basis for subsequent position and temperature calibration, avoiding manual measurement, and improving calibration accuracy and efficiency.

[0012] Other features and advantages of the present application will be described in detail in the subsequent detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The accompanying drawings are used to provide an understanding of the application and constitute a part of the specification. Together with the following detailed description, they are used to explain the application but do not constitute a limitation of the application. In the accompanying drawings:

[0014] FIG1 is a schematic structural diagram of a calibration device according to an embodiment of the present invention;

[0015] FIG2 is a schematic diagram of the exploded structure of a calibration device according to an embodiment of the application;

[0016] FIG3 is a schematic structural diagram of a calibration device according to another embodiment of the application;

[0017] FIG4 is a schematic structural diagram of a calibration device according to another embodiment of the application;

[0018] FIG5 is a schematic structural diagram of a front tightening member of a calibration device according to another embodiment of the application;

[0019] FIG6 is a schematic diagram of a partial structure of a calibration device according to an embodiment of the application;

[0020] 7 is a schematic structural diagram of a profiling frame of a calibration device according to an embodiment of the application;

[0021] FIG8 is a schematic structural diagram of a calibration device at a viewing angle according to an embodiment of the application;

[0022] FIG9 is a schematic structural diagram of a calibration device according to an embodiment of the application from another perspective;

[0023] FIG10 is a schematic diagram of the exploded structure of a calibration device according to an embodiment of the application;

[0024] FIG11 is a schematic diagram of the calibration main structure of the calibration device according to an embodiment of the application;

[0025] 12 is a schematic structural diagram of a position adjustment member of a calibration device according to an embodiment of the application;

[0026] FIG13 is a schematic structural diagram of a calibration device according to an embodiment of the application;

[0027] FIG14 is a schematic diagram of the exploded structure of a calibration device according to an embodiment of the application;

[0028] FIG15 is a control flow chart of a position calibration system according to an embodiment of the present application;

[0029] FIG16 is a control flow chart of a position calibration system according to another embodiment of the present application;

[0030] FIG17 is a schematic structural diagram of a position calibration system according to an embodiment of the present application.

[0031] Description of reference numerals:

[0032] 100, calibration device; 10, calibration body; 11, sliding cavity; 111, limiting portion; 12, electric control cavity; 13, mounting slot; 14, profiling frame; 15, bottom baffle; 16, inspection cover; 17, balancing portion; 18, avoidance groove; 19, profiling base; 10, temperature detection cavity; 101, upper mounting cavity; 20, first position calibration mechanism; 2, position adjustment member; 21, sliding body; 211, main body plate; 212, limiting ear; 213, hollow cavity; 22, limiting hook; 23, push ramp; 24, limiting portion; 25, baffle; 26, limiting block; 261, limiting column; 2 7. First detector; 28. Second detector; 30. Second position calibration mechanism; 31. Third detector; 4. First temperature detector; 41. Sensor body; 42. Detection telescopic head; 43. Thermal insulation sleeve; 44. Heat conducting part; 45. Thermal insulation board; 46. Cable cover; 5. Temperature probe; 51. Profiled flat tube; 52. Positioning mounting bracket; 6. Protective pressure plate; 7. Elastic reset member; 8. Circuit control board; 9. Wire clamp seat; 91. Wire clamp cover; 901. Limiting cover; 902. Wire trough cover; 903. Rear cover; 904. Lower cover; 200. Nucleic acid detection equipment; 210. Pipette tip; DETAILED DESCRIPTION

[0033] The following is a detailed description of the specific embodiments of the present application in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present application and are not intended to limit the present application.

[0034] The calibration device according to the present application is described below with reference to the accompanying drawings.

[0035] As shown in Figures 1 to 7, the calibration device 100 includes a calibration body 10, a first position calibration mechanism 20, and a temperature probe 5. The calibration body 10 is adapted for use with a POCT integrated device. The first position calibration mechanism 20 is mounted on the calibration body 10 and includes a position adjustment member 2 and a first detector 27. The position adjustment member 2 can cooperate with the first detector 27 to detect the Y coordinate of the amplification position under the push of the PCR module. The temperature probe 5 is removably mounted on the calibration body 10 and adapted for use with the PCR module to detect the heating temperature of the PCR module. In other embodiments, the temperature probe 5 is fixedly connected to the calibration body 10. The calibration body 10 can be designed to conform to the consumables of the POCT integrated device.

[0036] It is understood that the POCT all-in-one device in this embodiment can employ conventional nucleic acid detection equipment. The POCT all-in-one device can drive the calibration device 100 to move in the Y direction. The pipetting and extraction module is provided with a pipette tip 210. The translating member and the lifting member on the pipetting and extraction module can respectively drive the pipette tip 210 to move in the Y and Z directions. The Y direction can be the front-to-back direction in Figure 1, and the Z direction can be the lifting direction, i.e., the up-down direction, in Figure 1. The amplification position and the extraction position are arranged sequentially along the front-to-back direction, with the extraction position located behind the amplification position.

[0037] When using the calibration device 100 of this embodiment to calibrate the position of a POCT all-in-one device, the calibration device 100 and the POCT all-in-one device can be aligned and positioned. The POCT all-in-one device can drive the calibration device 100 toward the PCR module until it reaches the amplification position. The PCR module can then push against the position adjustment member 2, allowing the position adjustment member 2 to automatically detect the Y coordinate of the amplification position in conjunction with the first detector 27, facilitating subsequent position calibration of the amplification position. The temperature probe 5 is adapted to the PCR module and can automatically detect the heating temperature of the PCR module, facilitating subsequent temperature calibration of the PCR module. The calibration device 100 of this embodiment, by emulating the consumables of the POCT all-in-one device and cooperating with the PCR module and liquid extraction module of the POCT all-in-one device, can automatically detect the current coordinates of the amplification position and the heating temperature of the PCR module, thereby providing a precise calibration basis for subsequent position and temperature calibration, avoiding manual measurement and improving calibration accuracy and efficiency.

[0038] In one embodiment, a calibration device 100 is used to calibrate a POCT integrated device with a PCR module and a pipetting and extraction module. The calibration device 100 includes a calibration body 10, a first position calibration mechanism 20, and a temperature probe 5. The bottom of the calibration body 10 is provided with a limit slot adapted for the POCT integrated device. The first position calibration mechanism 20 is mounted on the calibration body 10 and includes a position adjustment member 2, a first detector 27, and a second detector 28. The position adjustment member 2 cooperates with the first detector 27 to detect the Y coordinate of the amplification position under the push of the PCR module. The second detector 28 cooperates with the pipetting and extraction module to detect the Y and Z coordinates of the extraction position. The temperature probe 5 is detachably mounted on the calibration body 10 and adapted to the PCR module to detect the heating temperature of the PCR module. In other embodiments, the temperature probe 5 is fixedly connected to the calibration body 10. The calibration body 10 can be designed to conform to the consumables of the POCT integrated device.

[0039] It is understood that the POCT all-in-one device in this embodiment can adopt the nucleic acid detection equipment used for nucleic acid detection in the prior art. The POCT all-in-one device can drive the calibration device 100 to move in the Y direction. The pipetting and extraction module is provided with a pipette tip. The translation member and the lifting member on the pipetting and extraction module can respectively drive the pipette tip to move in the Y direction and the Z direction. The Y direction can be the front-to-back direction in Figure 1, and the Z direction can be the lifting direction in Figure 1, that is, the up-down direction. The amplification position and the extraction position are arranged in sequence along the front-to-back direction, wherein the extraction position is located behind the amplification position.

[0040] When the calibration device 100 of this embodiment is used to calibrate the position of the POCT all-in-one device, the calibration device 100 and the POCT all-in-one device can be connected in a limit position via a limit slot. The POCT all-in-one device can drive the calibration device 100 toward the PCR module until it moves to the amplification position. The PCR module can reversely push the position adjustment member 2, so that the position adjustment member 2 cooperates with the first detector 27 to automatically detect the Y coordinate of the amplification position, which can facilitate subsequent position calibration of the amplification position. The temperature probe 5 is adapted to the PCR module and can automatically detect the heating temperature of the PCR module, which can facilitate subsequent temperature calibration of the PCR module. The POCT all-in-one device drives the calibration device 100 to the extraction position. The pipetting and extraction module moves along the Y direction and passes through the second detector 28. The second detector 28 can cooperate with the pipetting and extraction module to automatically detect the Y coordinate of the extraction position, which can facilitate subsequent calibration of the Y coordinate of the extraction position. The pipetting and extraction module moves along the Z direction and passes through the second detector 28. The second detector 28 can cooperate with the pipetting and extraction module to automatically detect the Z coordinate of the extraction position. The current Z coordinate of the extraction position can facilitate subsequent calibration of the Z coordinate of the extraction position. The calibration device 100 in this embodiment can automatically detect the current coordinates of the amplification position and the extraction position by imitating the consumables of the POCT all-in-one machine and cooperating with the PCR module and the pipetting extraction module of the POCT all-in-one machine, thereby providing an accurate calibration basis for subsequent position calibration, avoiding manual measurement, and improving calibration accuracy and efficiency.

[0041] Position and temperature calibration can be automatically performed using a pre-programmed program and a set position and temperature. This eliminates the need for specialized calibration expertise, improving the user experience and making operation more convenient.

[0042] Specifically, the position adjustment member 2 includes a sliding body 21 and two limiting hooks 22. The calibration body 10 is provided with a sliding cavity 11. The sliding body 21 is accommodated in the sliding cavity 11 and can slide back and forth between an initial position and a detection position relative to the sliding cavity 11. The two limiting hooks 22 are provided at both ends of the sliding body 21 along the Y direction and cooperate with the calibration body 10 to limit the position. In this embodiment, the limiting hooks 22 are provided at both ends of the sliding body 21. When the PCR module pushes the sliding body 21 to slide along the Y direction, the limiting hooks 22 can cooperate with the calibration body 10 to limit the position, thereby preventing the sliding body 21 from separating from the calibration body 10, thereby facilitating subsequent calibration of the POCT all-in-one device.

[0043] As shown in Figures 2 and 6, the sliding body 21 includes a main plate 211 and a limiting lug 212. The main plate 211 extends in the Y direction. The limiting lug 212 protrudes from the edge of the main plate 211 in the Z direction. The sliding cavity 11 is provided with a limiting portion 111 corresponding to the limiting lug 212. The size of the limiting portion 111 is larger than the size of the limiting lug 212. In this embodiment, there can be two limiting lugs 212, which are located on either side of the main plate 211 in the vertical direction. When the sliding body 21 slides in the Y direction, the limiting lugs 212 can slide along the main plate 211 in the Y direction within the limiting portion 111, and the cavity wall of the limiting portion 111 can abut against the limiting lug 212 in the Y direction. In this embodiment, the limiting lug 212 can cooperate with the limiting hook 22 to limit the position of the position adjustment member 2 in the Y direction, ensuring that the position adjustment member 2 is always in the set position in the Y direction, thereby improving the operational stability of the calibration device 100.

[0044] As shown in Figures 1 to 4, the sliding cavity 11 is provided with an assembly opening, and the first position calibration mechanism 20 further includes a protective pressure plate 6 disposed at the assembly opening and detachably connected to the calibration body 10. The protective pressure plate 6 is used to protect the position adjustment member 2 and the first detector 27. In this embodiment, the protective pressure plate 6 is detachably connected to the calibration body 10. When the protective pressure plate 6 completely covers the first detector 27, it can protect the first detector 27 and the position adjustment member 2. The first detector 27 and the position adjustment member 2 can be installed or replaced by removing the protective pressure plate 6, thereby improving the maintenance convenience of the first detector 27 and the position adjustment member 2.

[0045] It should be noted that the first position calibration mechanism 20 also includes an elastic reset member 7 for driving the position adjustment member 2 to reset. The elastic reset member 7 is connected between the calibration body 10 and the position adjustment member 2, or the elastic reset member 7 is connected between the first detector 27 and the position adjustment member 2. The elastic reset member 7 can be a spring, and the number of elastic reset members 7 can be specifically set according to the use requirements. As shown in Figures 2 and 3, in one embodiment, the first detector 27 extends in the left-right direction, and the elastic reset member 7 is connected between the first detector 27 and the position adjustment member 2, resulting in a compact structure. As shown in Figures 4 and 5, in another embodiment, the first detector 27 extends in the front-to-back direction, and the elastic reset member 7 is connected between the calibration body 10 and the position adjustment member 2, which can reduce the length of the position adjustment member 2 and achieve miniaturization of the calibration device 100.

[0046] As shown in Figure 2, the calibration body 10 is provided with an electric control cavity 12, and the calibration device 100 also includes a circuit control board 8 installed in the electric control cavity 12 and integrated with a power supply module and a communication module. The first detector 27, the second detector 28 and the temperature probe 5 are all electrically connected to the circuit control board 8. The circuit control board 8 in this embodiment can supply power to the first detector 27, the second detector 28 and the temperature probe 5, and the position signal and temperature signal detected by the first detector 27, the second detector 28 and the temperature probe 5 can be sent to the POCT all-in-one machine through the circuit control board 8. In this embodiment, the electric control cavity 12 is located on the left side of the calibration body 10, and the position adjustment component 2 is located on the right side of the calibration body 10, which can balance the weight of the calibration device 100 and avoid the problem that the calibration device 100 is difficult to place due to an unstable center of gravity. In order to further ensure that the center of gravity of the calibration device 100 is centered, the calibration body 10 is also provided with an outwardly protruding balancing portion 17, which is arranged on the same side as the electric control cavity 12.

[0047] It should be noted that a clearance groove 18 is defined at the top of the calibration body 10, and the second detector 28 is installed within the clearance groove 18 to prevent interference with the second detector 28 by other components. In one embodiment, the calibration body 10 is provided with mounting slots 13 that engage with the circuit control board 8. The two mounting slots 13 are located on either side of the circuit control board 8 along the Y direction. The mounting slots 13 in this embodiment extend in the vertical direction. When the circuit control board 8 extends into the electrical control cavity 12, the mounting slots 13 not only guide the circuit control board 8 but also engage the circuit control board 8, achieving initial installation and positioning of the circuit control board 8 and facilitating subsequent circuit wiring.

[0048] Specifically, the calibration body 10 includes a contour frame 14 and a bottom baffle 15 detachably connected to the contour frame 14. The electric control chamber 12 is disposed on the contour frame 14. The bottom baffle 15 is used to seal the electric control chamber 12, and a limit slot is disposed on the bottom baffle 15. In this embodiment, the bottom baffle 15 is detachably connected to the contour frame 14, allowing different bottom baffles 15 to be replaced for different POCT all-in-one devices, thereby improving the compatibility of the calibration device 100. It is understood that the calibration body 10 also includes an inspection cover 16 detachably connected to the contour frame 14. The electric control chamber 12 is provided with an inspection port. The inspection cover 16 is disposed at the inspection port and is used to open or close the inspection port. The inspection cover 16 not only seals the circuit control board 8 but also facilitates inspection, observation, and cable connection of the circuit control board 8.

[0049] In one embodiment, a wire clamp 9 for securing the connecting wires is mounted on the end of the calibration body 10 away from the temperature probe 5. The temperature probe 5 includes a contoured flat tube 51 and a positioning bracket 52 that is removably mounted on the calibration body 10. The wire clamp 9 in this embodiment secures the connecting wires, facilitating portability and use of the calibration device 100. In other embodiments, the positioning bracket 52 is fixedly connected to the calibration body 10.

[0050] As shown in Figures 8 to 12, in some embodiments, the calibration device 100 includes a calibration body 10, a first position calibration mechanism 20 and a second position calibration mechanism 30. The calibration body 10 is provided with a contoured base 19 adapted to the POCT all-in-one machine; the first position calibration mechanism 20 is installed on the calibration body 10 and includes a position adjustment component 2, a first detector 27 and a second detector 28. The position adjustment component 2 can slide relative to the calibration body 10 under the push of the PCR module, and cooperate with the first detector 27 to detect the Y-axis coordinate of the amplification position; the second position calibration mechanism 30 is installed on the calibration body 10 and includes a third detector 31. The third detector 31 is used to cooperate with the pipetting extraction module to detect the rotation coordinates of the extraction position, and the second detector 28 is used to cooperate with the pipetting extraction module to detect the Y-axis coordinate and Z-axis coordinate of the extraction position.

[0051] The calibration device 100 in this embodiment is primarily used for position and temperature calibration of a POCT all-in-one device. The POCT all-in-one device uses a conventional structure and is primarily used for point-of-care testing. The POCT all-in-one device can drive the calibration device 100 in the Y direction. The pipetting and extraction module includes a pipette tip and a rotating, lifting, and translating member that drive the pipette tip's movement. The rotating member drives the pipette tip to rotate horizontally, the lifting member drives the pipette tip to rise and fall in the Z direction, and the translating member drives the pipette tip to translate in the Y direction. The X direction is the left-right direction in Figure 8, i.e., the width of the calibration device 100. The Y direction is the front-to-back direction in Figure 8, i.e., the length of the calibration device 100. The Z direction is the top-to-bottom direction in Figure 8, i.e., the height of the calibration device 100. The pipetting and extraction module can rotate horizontally. The amplification and extraction positions are sequentially arranged along the front-to-back direction. The first position calibration mechanism 20 can be internally mounted within the calibration body 10, while the second position calibration mechanism 30 can be exposed externally from the calibration body 10. The contoured base 19 can be disposed at the bottom of the calibration body 10.

[0052] When the calibration device 100 in this embodiment is used to calibrate the POCT all-in-one machine, the contoured base 19 of the calibration body 10 and the POCT all-in-one machine can be snap-fitted and adapted so that the calibration device 100 can move as a whole in the Y direction under the drive of the POCT all-in-one machine until the calibration device 100 moves to the amplification position on the POCT all-in-one machine. During the movement, the PCR module of the POCT all-in-one machine can push the position adjustment component 2, so that the position adjustment component 2 moves in the Y direction relative to the calibration body 10 and triggers the position detection of the first detector 27, so that the position adjustment component 2 and the first detector 27 cooperate to detect the Y coordinate of the amplification position. After obtaining the current Y coordinate of the amplification position, it is convenient to calibrate the amplification position. The POCT all-in-one machine drives the pipetting extraction module in During the rotation of the horizontal plane, the position detection of the third detector 31 can be triggered, so that the third detector 31 cooperates with the pipetting and extraction module to detect the current rotation coordinates of the extraction position, which is convenient for the subsequent calibration of the rotation coordinates of the pipetting and extraction module. After the POCT all-in-one machine drives the pipetting and extraction module to descend to the predetermined position, it can drive the calibration device 100 to move along the Y direction to the extraction position. The pipetting and extraction module can trigger the position detection of the second detector 28, so that the second detector 28 detects the current Y coordinate of the extraction position, which is convenient for the subsequent calibration of the Y coordinate of the extraction position. The POCT all-in-one machine drives the pipetting and extraction module to move upward, which can trigger the position detection of the second detector 28, so that the second detector 28 detects the current Z coordinate of the extraction position, which is convenient for the subsequent calibration of the Z coordinate of the extraction position. The calibration device 100 in this embodiment is modeled after the detection consumables of the POCT all-in-one machine. Through the adaptation of the modeling base 19 and the POCT all-in-one machine, the calibration device 100 can automatically detect the current coordinates of the amplification position and the extraction position under the drive of the POCT all-in-one machine, thereby avoiding the situation of manual operation of the detection, greatly improving the detection efficiency and consistency, and at the same time, without the need for strong professionalism, and can meet the customer's self-calibration needs, thereby reducing after-sales costs.

[0053] As shown in Figure 11, the calibration body 10 defines a temperature detection chamber 10. The calibration device 100 also includes a first temperature detector 4 disposed within the temperature detection chamber 10. One end of the first temperature detector 4 is recessed within the temperature detection chamber 10, while the other end protrudes from the temperature detection chamber 10 and is used to detect the temperature of the heating module of the POCT all-in-one device. In this embodiment, the first temperature detector 4 can be a temperature sensor. The heating module of the POCT all-in-one device is located at the bottom of the POCT all-in-one device. The first temperature detector 4 extends vertically and penetrates the contoured base 19. The first temperature detector 4 is recessed within the temperature detection chamber 10 to prevent interference between other components and the first temperature detector 4. Heating is continuously performed during the calibration process. After the Z-axis coordinate of the extraction position is calibrated, the first temperature detector 4 can automatically detect the current heating temperature of the heating module. The operator can determine whether the heating module needs to be calibrated by comparing the current heating temperature with the preset heating temperature. The calibration device 100 in this embodiment integrates temperature calibration and position calibration, which greatly improves operational convenience, avoids the need for separate position and temperature calibration, and improves calibration efficiency.

[0054] Specifically, the first temperature detector 4 includes a sensor body 41 housed in the temperature detection cavity 10 and a detection expansion head 42 protruding from the temperature detection cavity 10. An elastic avoidance member is connected between the detection expansion head 42 and the sensor body 41. The elastic avoidance member in this embodiment can be a spring. The heating module can squeeze the detection expansion head 42, causing the detection expansion head 42 to compress the elastic avoidance member. The elastic avoidance member provides a downward reaction force to the detection expansion head 42, ensuring close contact between the detection expansion head 42 and the heating module, thereby avoiding loose contact between the detection expansion head 42 and the heating module, which may lead to inaccurate temperature detection.

[0055] As shown in Figure 10, the first temperature detector 4 also includes a thermal insulation sleeve 43 that is sleeved on the outside of the sensor body 41. A mounting port is provided at the top of the temperature detection cavity 10, and a limit cover 901 is detachably installed at the mounting port. The limit cover 901 is used to limit the thermal insulation sleeve 43 within the temperature detection cavity 10. The limit cover 901 and the calibration body 10 in this embodiment are detachably connected, and the limit cover 901 is U-shaped. The sensor body 41 can be first inserted into the thermal insulation sleeve 43, and then the first temperature detector 4 can be inserted into the temperature detection cavity 10 as a whole through the mounting port. Finally, the limit cover 901 is connected to the calibration body 10 so that the limit cover 901 completely covers the mounting port, which can prevent the first temperature detector 4 from falling out. The temperature detection cavity 10 in this embodiment is cylindrical.

[0056] In one embodiment, the calibration device 100 further includes a temperature probe 6 mounted on the calibration body 10 and configured to monitor the temperature of the PCR module. The shape of the temperature probe 6 is adapted to the shape of the detection slot of the PCR module. The temperature probe 6 is mounted on the front end of the calibration body 10. Driven by the POCT all-in-one device, the calibration device 100 moves to the amplification position, where the temperature probe 6 can be inserted into the detection slot of the PCR module. The temperature probe 6 automatically monitors the current heating temperature of the PCR module, allowing the operator to calibrate the PCR module's heating temperature based on the current heating temperature.

[0057] It should be noted that the temperature probe 6 includes a positioning mounting frame 52 and a contoured flat tube 51. The positioning mounting frame 52 is detachably connected to the calibration body 10; the contoured flat tube 51 is provided with a second temperature detector and is detachably connected to the positioning mounting frame 52. When it is necessary to calibrate the heating temperature of the PCR module separately, the temperature probe 6 can be removed from the calibration body 10 as a whole, making it convenient to remove it independently for external calibration. The second temperature detector can be a temperature sensor. The front end of the contoured flat tube 51 is provided with a diamond-shaped cavity, and the second temperature detector can be installed in the diamond-shaped cavity. When calibrating different models of POCT all-in-one machines, the contoured flat tube 51 can be removed relative to the positioning mounting frame 52 and replaced with a contoured flat tube 51 of the corresponding model, so that the calibration device 100 can be calibrated for different models of POCT all-in-one machines, thereby improving the compatibility of the calibration device 100.

[0058] In the embodiment of the present application, an electric control cavity 12 is provided at the end of the calibration body 10 away from the temperature probe 6. The calibration device 100 also includes a circuit control board 8 installed in the electric control cavity 12. The circuit control board 8 is integrated with a power supply module and a communication module, and the first detector 27, the third detector 31, the second detector 28, and the temperature probe 6 are all electrically connected to the circuit control board 8. In this embodiment, the rear end of the calibration body 10 is provided with an inwardly recessed electric control cavity 12. The power supply module on the circuit control board 8 can supply power to the first detector 27, the third detector 31, the second detector 28, and the temperature probe 6. The communication module can communicate with the POCT all-in-one machine and send the coordinates detected by the first detector 27, the third detector 31, and the second detector 28, and the heating temperature detected by the temperature probe 6 to the POCT all-in-one machine, thereby facilitating the position calibration and temperature calibration of the POCT all-in-one machine.

[0059] It should be noted that the calibration device 100 also includes an elastic reset member 7, which is located between the position adjustment member 2 and the calibration body 10. The position adjustment member 2 cooperates with the calibration body 10 to compress the elastic reset member 7 under the push of the PCR module. The elastic reset member 7 is used to reset the position adjustment member 2. The elastic reset member 7 in this embodiment can be a spring. When the PCR module pushes the position adjustment member 2, the position adjustment member 2 can compress the elastic reset member 7 toward the calibration body 10. When the calibration device 100 is separated from the PCR module, the elastic reset member 7 can automatically reset the position adjustment member 2 through its own elastic force, thereby facilitating subsequent calibration operations of other POCT all-in-one devices.

[0060] As shown in Figure 12, the calibration body 10 is provided with a sliding cavity, and the position adjustment member 2 is accommodated in the sliding cavity. A telescopic opening is provided at one end of the sliding cavity, and a limit stop 26 is provided at the other end of the sliding cavity to limit the position adjustment member 2. The telescopic opening is located at the front end of the sliding cavity, so that the front end of the sliding cavity is open, and the limit stop 26 is located at the rear end of the sliding cavity. In one embodiment, the position adjustment member 2 can always protrude from the calibration body 10 relative to the sliding cavity under the support of the elastic reset member 7, and push against the PCR module in an exposed manner. In another embodiment, the front end of the position adjustment member 2 in this embodiment is provided with a push inclined surface 23 inclined toward the rear end, which can facilitate the push between the PCR module and the position adjustment member 2.

[0061] The limit stopper 26 in this embodiment is L-shaped and includes a front baffle and a lower baffle. The front baffle can limit the position of the limit portion 212 on the detection member 2 in the Y direction, while the lower baffle can support the limit connection line, thereby facilitating the line passing. In one embodiment, there are two limit portions 212, and a baffle 213 is disposed between the two limit portions 212 to cooperate with the first detector 27. The shapes of the two limit portions 212 can be the same or different depending on actual usage requirements.

[0062] In one embodiment, the first detector 27, the third detector 31, and the second detector 28 are all photoelectric sensors. An upper mounting cavity 101 is defined at the upper end of the calibration body 10, and the third detector 31 and the second detector 28 are housed within the upper mounting cavity 101. The upper mounting cavity 101 is located at the upper end of the calibration body 10 and is open. The upper surfaces of the third detector 31 and the second detector 28 do not protrude beyond the upper edge of the upper mounting cavity 101, thereby preventing interference between the third detector 31 and the second detector 28 and other components. A position adjustment component can pass through the photoelectric detection port of the first detector 27, and a pipette can pass through the photoelectric detection ports of the third detector 31 and the second detector 28, generating photoelectric signal changes, thereby achieving photoelectric detection.

[0063] In this embodiment, the rear end of the calibration body 10 can be installed with a wire clamp seat 9 with a wire clamp cover 91, and the connecting wire is fixed by the wire clamp seat 9, which is convenient for carrying and use; a wire groove is provided on the top of the calibration body 10, and the wire groove is completely covered by the wire groove cover 902, which can avoid the connecting wire from interfering with other components. A circuit inspection port is provided at the rear end of the electric control cavity 12, and the outer cover of the circuit control board 8 is provided with a rear cover 903 for sealing the circuit inspection port. The rear cover 903 and the calibration body 10 are detachably connected. A lower installation port is provided at the lower end of the calibration body 10 corresponding to the first detector 27, and the lower installation port is sealed with a lower cover 904. The lower cover 904 and the calibration body 10 are detachably connected, which can facilitate the installation and replacement of the first detector 27.

[0064] As shown in Figures 13 and 14, in one embodiment, a limiting post 261 is provided on the side of the limiting block 26 facing the position adjustment member 2; the elastic reset member 7 is arranged around the outer circumference of the limiting post 261 and elastically abuts the position adjustment member 2 at one end facing away from the limiting block 26. When calibrating the amplified position, the elastic reset member 7 extends in the left-right direction and is elastically connected to the position adjustment member 2, thereby providing a moving force to the position adjustment member 2. The limiting post 261 is fixed to the limiting block 26 by screws, which can reduce the difficulty of processing and is also easy to replace. The elastic reset member 7 is sleeved on the limiting post 261, so that the limiting post 261 can provide a radial constraint to the elastic reset member 7, so that the elastic reset member 7 is in a relatively stable state whether during assembly or movement. When not in contact with the PCR module, the limiting post 261 maintains a certain distance from the end of the position adjustment component 2. When the PCR module pushes the position adjustment component 2, the position adjustment component 2 slowly moves to the left under the buffering action of the elastic reset component 7 until the position adjustment component 2 contacts the limiting post 261 and stops moving. That is to say, the limiting post 261 acts on the position adjustment component 2, limiting the distance of movement to the left of the position adjustment component 2.

[0065] As shown in Figure 14, the position adjustment member 2 has a hollow cavity 213 extending in the longitudinal direction at one end facing the elastic return member 7, and the other end is closed. The end of the elastic return member 7 facing away from the limit stop 26 is inserted into the hollow cavity 213. The elastic return member 7 is able to remain extended in the left-right direction under the restraining action of the limit post 261. Inserting the right end of the elastic return member 7 into the hollow cavity 213 ensures that the position adjustment member 2 remains connected to the elastic return member 7, ensuring the stability of the position adjustment member 2 during left-right movement.

[0066] The first temperature detector 4 includes a heat conducting portion 44, a temperature sensor, and a heat insulating plate 45. The temperature sensor is arranged in the heat conducting portion 44. One end of the heat conducting portion 44 passes through the heat insulating plate 45 and is inserted into the calibration body 10. During the temperature detection process, the heat conducting portion 44 is brought into contact with the heating module. The heat of the heating module is transferred to the internal temperature sensor through the heat conducting portion 44. The temperature sensor can sense the temperature signal and transmit it, thereby realizing the temperature detection of the heating module. In order to prevent the calibration body 10 from being damaged due to the excessive temperature of the heat conducting portion 44 and direct contact with the calibration body 10 during the temperature detection process, a heat insulating plate 45 is provided on the side of the heat conducting portion 44 facing the calibration body 10. The provision of the heat insulating plate 45 can block the heat of the heat conducting portion 44 from being transferred to the calibration body 10, thereby protecting the calibration body 10.

[0067] In the embodiment of the present application, a first cable cover 46 is further provided on the periphery of the thermal insulation plate 45. The first cable cover 46 and the calibration body 10 cooperate to wrap around the periphery of the thermal insulation plate 45 and the heat conducting portion 44, thereby protecting the thermal insulation plate 45 and the heat conducting portion 44 and improving the service life of the entire first temperature detector 4. In addition, a second cable cover is also provided on the periphery of the first temperature detector 4 to prevent frequent contact between the first temperature detector 4 and the calibration body 10 during use, thereby shortening the service life. Furthermore, the temperature sensor cable can be electrically connected to the circuit control board 8 within the calibration body 10 via the first cable cover 46 and the second cable cover, thereby ensuring normal transmission of the temperature detection signal.

[0068] The present application also proposes a position calibration system for a nucleic acid detection device 200 with a motion module. The position calibration system includes a calibration device 100 and an analysis device. The calibration device 100 is adapted to the nucleic acid detection device 200 and is used to detect the motion module; the analysis device, the calibration device 100 and the nucleic acid detection device 200 are both communicatively connected to the analysis device.

[0069] As shown in FIG15 , in one embodiment, the analysis device is configured to:

[0070] Obtaining calibration input information and device information of the nucleic acid detection device 200;

[0071] Combine the calibration input information and device information to obtain the position to be calibrated and the calibration operation instructions;

[0072] Control the motion module to drive the calibration device to move to the position to be calibrated;

[0073] Start calibration and send calibration operation instructions to the nucleic acid detection equipment 200.

[0074] It can be understood that the calibration input information can be human-machine instructions or network instructions, etc., and the analysis device can collect human-machine instructions through the touch screen or instruction buttons. The standard mode in this embodiment can be pre-set, and the corresponding standard mode is preset according to different equipment types. The nucleic acid detection device 200 can be a POCT all-in-one machine. The motion module can drive the calibration device 100 to move along the Y-axis, that is, the front and back direction. The nucleic acid detection device 200 also includes a pipetting module with a pipette tip 210, a device control module, a communication module, and a detection reaction module. The analysis device can issue calibration operation instructions to the device control module, and the device control module can generate motion module control instructions during the position calibration process according to the calibration operation instructions. The motion module may include a motion motor. The calibration device 100 adopts a contoured design and is adapted to the nucleic acid detection device 200.

[0075] In addition, according to whether the nucleic acid detection device 200 can perform rotational motion, the nucleic acid detection device 200 can be divided into device types with S-axis drive and without S-axis drive. In one embodiment, the pipetting module includes a pipetting pump with a pipette tip 210, and the motion module in this embodiment also includes a Y-axis drive, a Z-axis drive, and an S-axis drive, wherein the Z-axis drive and the S-axis drive are all connected to the pipetting module, and the Y-axis drive, the Z-axis drive, and the S-axis drive can all use stepper motors. The Z-axis drive can drive the pipette tip 210 in the up and down directions, and the S-axis drive can drive the pipette tip 210 to rotate along the horizontal plane.

[0076] When the position calibration system in this embodiment is used for position calibration, the calibration device 100 can be placed in the consumables position of the nucleic acid detection device 200 first, and the calibration device 100, the nucleic acid detection device 200 and the analysis device are communicatively connected. When the analysis device determines that the calibration device 100 has entered the nucleic acid detection device 200, the calibration input information can be obtained. The analysis device can obtain the position to be calibrated and the calibration operation instructions based on the calibration input information and the device information of the nucleic acid detection device 200. It has high compatibility for different types of equipment and can control the motion module to drive the calibration device to move to the position to be calibrated according to the calibration operation instructions. It does not rely on the user's professional skills and can achieve automatic control with high accuracy. The calibration operation instructions are sent to the nucleic acid detection device 200, which can control the motion module to further perform precise calibration movements. The position calibration system in this embodiment combines the device information and calibration input information of the nucleic acid detection device 200 to automatically calibrate the positions to be calibrated of different types of nucleic acid detection devices 200, and has high calibration compatibility, calibration accuracy and calibration efficiency.

[0077] Specifically, the calibration input information includes a mode input instruction. As shown in FIG16 , the analysis device is further configured to:

[0078] Determine that the mode input instruction includes the standard mode, obtain the calibration operation instruction and the position to be calibrated according to the device information;

[0079] The mode input instruction is determined to include a custom mode, and the calibration operation instruction and the position to be calibrated are obtained according to the calibration input information.

[0080] The calibration mode is confirmed. When it is determined that the calibration mode is the standard mode, the standard mode is the standard mode of the nucleic acid detection device 200. The calibration operation instruction can be obtained according to the device information of the nucleic acid detection device 200. The device information may include the device type and historical calibration operation data. The analysis device can control the motion module to drive the calibration device 100 to move according to the calibration operation instruction, and determine the calibration value of the motion module. The position calibration system in this embodiment can provide users with the ability to select a calibration mode. The user does not need to have high professional skills, and the calibration flexibility is good. The calibration mode is obtained by identifying and calibrating the input information of the nucleic acid detection device 200. Standardized calibration or custom calibration can be performed, and the position calibration requirements of different calibration scenarios, different calibration equipment, and different calibration modes are met, reducing the difficulty of after-sales maintenance to be calibrated.

[0081] In one embodiment, the calibration input information further includes a position input instruction, and the analysis device is further configured to:

[0082] Determine that the calibration input information includes a custom mode and obtain position input instructions;

[0083] The custom options are determined according to the position input instruction, and the custom options include at least one or more of the Y-axis amplification bit, the Y-axis extraction bit, the Z-axis extraction bit, and the S-axis extraction bit.

[0084] In this embodiment, the Y-axis is the front-to-back direction, the Z-axis is the up-down direction, and the S-axis extraction position is used to calibrate the rotational position of the pipette tip 210 in the horizontal plane. When inputting the calibration input information in a human-computer interaction manner, the user can select the Y-axis amplification position, the Y-axis extraction position, the Z-axis extraction position, and the S-axis extraction position according to different needs, and can achieve different positions. When a single position needs to be calibrated, only a single position selection can be made. When multiple positions need to be calibrated, multiple positions can be selected, further improving the compatibility of the position calibration system. After the custom options are selected, the analysis device can generate corresponding calibration operation instructions corresponding to different custom options, and control the nucleic acid detection equipment to perform calibration operations in the corresponding order according to the calibration operation instructions.

[0085] In one embodiment, the analyzing device is further configured to:

[0086] Obtaining current position detection information of the calibration device on the position to be calibrated;

[0087] Calculate the calibration deviation value of the position to be calibrated;

[0088] It is determined that the calibration deviation value is less than the preset deviation value, and the current position of the position to be calibrated is marked as the calibration position.

[0089] The analysis device in this embodiment can obtain the default position information and current position detection information of the position to be calibrated based on the detection of the motion module of the calibration device, and calculate the calibration deviation value. If the calibration deviation value is determined to be less than the preset deviation value, the current position of the position to be calibrated is marked as the calibration position. If the calibration deviation value is greater than or equal to the preset deviation value, the calibration has failed, and the analysis device can issue a calibration failure alarm. The analysis device can also control the motion module to reset and shut down the motion module.

[0090] In one embodiment, the bits to be calibrated include at least Y-axis amplified bits and Z-axis extracted bits.

[0091] As shown in Figures 1 to 17, the calibration device 100 includes a calibration body 10 and a first position calibration mechanism 20 installed on the calibration body 10. The calibration body 10 is adapted to the nucleic acid detection equipment 200. The first position calibration mechanism 20 includes a position adjustment component 2 and a first detector 27. The position adjustment component 2 cooperates with the first detector 27 under the push of the nucleic acid detection equipment 200 and detects the current position of the Y-axis amplification position. The second detector 28 is used to detect the current position of the Z-axis extraction position.

[0092] In this embodiment, the first detector 27 and the second detector 28 can be photoelectric sensors, which detect position through photoelectric induction. The position to be calibrated also includes the Y-axis extraction position and the S-axis extraction position. The second detector 28 is also used to detect the current position of the Y-axis extraction position. The first position calibration mechanism 20 also includes an elastic reset member 7, which is located between the position adjustment member 2 and the calibration body 10. The position adjustment member 2 can cooperate with the calibration body 10 to compress the elastic reset member 7 under the push of the PCR module, and the elastic reset member 7 can drive the position adjustment member 2 to reset.

[0093] In one embodiment, the first position calibration mechanism 20 further includes a third detector 31, which may be a photoelectric sensor for detecting the current position of the S-axis extraction position. The photoelectric sensor detects a position with a state of 0, where 1 represents being blocked by the pipette tip 210 and 0 represents not being blocked by the pipette tip 210. The first detector 27, the second detector 28, and the third detector 31 are all photoelectric sensors; the second detector 28 can also be used to detect the motion module of the Y-axis extraction position. An upper mounting cavity 101 is defined at the upper end of the calibration body 10, and the second detector 28 and the third detector 31 are both housed within the upper mounting cavity 101.

[0094] In one embodiment, the analyzing device is further configured to:

[0095] Determining that the first position calibration mechanism 20 is not in position, and controlling the motion module to perform stepping motion toward a preset position;

[0096] Determine that the current position exceeds the preset position and shut down the motion module;

[0097] It is determined that the current position does not exceed the preset position and the first position calibration mechanism 20 is set, and the current position of the first position calibration mechanism 20 is marked as the calibration position.

[0098] The preset position in this embodiment can be set according to actual usage requirements. If the current position is determined to be outside the preset position, calibration has failed, and the analysis device can issue a calibration failure alarm. If the current position is determined to be within the preset position and the first position calibration mechanism 20 is in position, the current position of the first position calibration mechanism 20 is marked as the calibration position.

[0099] It should be noted that the analysis device is configured to:

[0100] Obtain the number of calibration items and calibration sequence in the calibration operation instruction;

[0101] Calibrate operations in sequence according to the calibration order;

[0102] Feedback completes the calibration instruction.

[0103] In one embodiment, after the motion module is reset, it is determined in sequence whether to perform Y-axis amplification position calibration, Y-axis extraction position calibration, Z-axis extraction position calibration, and S-axis extraction position calibration, and the initial position of the position to be calibrated is obtained. During the Y-axis amplification position calibration process, the analysis device first sends the Y-axis amplification initial position to the motion module. The Y-axis amplification initial position is equal to the Y-axis amplification default value + the Y-axis calibration range value, so that the calibration device 100 moves to the Y-axis amplification initial position and determines whether the first position calibration mechanism 20 is in position. If the first position calibration mechanism 20 is in position when it is first determined, a Y-axis calibration out-of-range alarm is issued.

[0104] Specifically, the analyzing device is further configured to:

[0105] Determine that the first position calibration mechanism 20 is not in position, and control the motion module to perform stepping motion from the Y-axis amplification initial position to the Y-axis amplification preset position;

[0106] Determine that the current position exceeds the preset position of the Y-axis expansion, shut down the motion module and issue a calibration failure alarm.

[0107] During the Y-axis amplification position calibration process, the analysis device controls the motion module to drive the calibration device 100 to move to the Y-axis amplification initial position, and determines whether the first position calibration mechanism 20 is in position. When the first position calibration mechanism 20 is not in position, the analysis device controls the motion module to step from the Y-axis amplification initial position to the Y-axis amplification preset position, adjusts with the minimum adjustment distance, and determines whether the current position exceeds the Y-axis amplification preset position. The Y-axis amplification preset position is equal to the Y-axis amplification default value - the amplification calibration range value. When the current position exceeds the Y-axis amplification preset position, the analysis device shuts down the motion module and issues a calibration failure alarm. In this embodiment, when the calibration device 100 exceeds the preset position, it can be determined that the calibration deviation value of the motion module is large, thereby avoiding the situation where the nucleic acid detection device 200 cannot be used normally due to calibration when the calibration deviation value is large.

[0108] During the Y-axis amplification position calibration process, the analysis device controls the motion module to perform stepping motion from the initial position to the preset position, adjusts it with the minimum adjustment distance, and determines whether the current position exceeds the Y-axis amplification preset position. When the current position does not exceed the Y-axis amplification preset position and the first position calibration mechanism 20 is in position, the current position of the Y-axis is recorded as the current calibration value of the Y-axis, the Y-axis amplification position calibration is successful, and the amplification position calibration process is completed. In this embodiment, the calibration device 100 is driven to perform stepping motion with the minimum adjustment distance, combined with photoelectric position detection, which can greatly improve the accuracy of the position calibration system. Even if the user does not have high professional skills, high-precision calibration of the nucleic acid detection equipment 200 can be achieved, which greatly improves the convenience of after-sales maintenance of the nucleic acid detection equipment 200.

[0109] In this embodiment, the amplification position and extraction position of the nucleic acid detection device 200 can be automatically calibrated with one button through precise motion control of the stepping motor and precise position information obtained by the photoelectric sensor. The position calibration accuracy is within ±0.2 mm.

[0110] The analysis device controls the motion module to perform Z-axis extraction position calibration. The analysis device can first control the motion module to move along the Y-axis to the Y-axis extraction position, and then determine the device type of the nucleic acid detection device 200, whether it is a nucleic acid detection device 200 with S-axis drive. When it is determined that the nucleic acid detection device 200 is a device type without S-axis drive, the motion module is controlled to drive the pipette tip 210 along the Z-axis to the Z-axis initial position. The Z-axis initial position is equal to the Z-axis extraction default value + Z-axis calibration range value. After the calibration device 100 is in place, it is determined whether the first position calibration mechanism 20 is in place, that is, whether the first position calibration mechanism 20 generates a photoelectric detection signal in place. When the first position calibration mechanism 20 is in the Z-axis initial position and is not in place, the analysis device can issue a Z-axis calibration out-of-range alarm.

[0111] When the first position calibration mechanism 20 is in its Z-axis initial position and in position, the analysis device can control the Z-axis drive of the motion module to drive the calibration device 100 upward in a stepping motion at a minimum adjustment distance, and determine that the current position of the motion module does not exceed a preset Z-axis position, where the preset Z-axis position is equal to the Z-axis extraction default value minus the calibration range value. If the current position of the motion module does not exceed the preset Z-axis position and the first position calibration mechanism 20 is not in position, the current position of the motion module is recorded as the calibration value.

[0112] In the embodiment of the present application, the nucleic acid detection device 200 further includes an experimental module for sample detection. The experimental module in this embodiment is a PCR module, and the analysis device is further configured as follows:

[0113] Make sure that the calibration device 100 enters the nucleic acid detection equipment 200 and locks the experimental module.

[0114] The nucleic acid detection equipment 200 in this embodiment can detect the calibration device 100 and send tooling in place information to the analysis device. The analysis device determines that the calibration device 100 is in place, locks the experimental module, and prohibits experimental operations of the experimental module, thereby avoiding the user's misoperation of the nucleic acid detection equipment 200 during the calibration process, thereby improving the calibration safety of the calibration system.

[0115] In an embodiment of the present application, the analysis device is integrated into one of the calibration device 100 or the nucleic acid detection equipment 200.

[0116] In one embodiment, the analysis device, the calibration device 100 and the nucleic acid detection device 200 are independent of each other. The analysis device is set separately, and the position analysis operation device can be a computer, notebook or mobile phone. The position analysis operation device may include a control module, a display interaction module, a storage module, a communication module and a device identification module. The device identification module can analyze the device type, usage and historical calibration information of the nucleic acid detection device 200 through the device information reported by the nucleic acid detection device 200. The display interaction module can display the relevant operating steps and parameters of this position calibration through the user interface, and can obtain the user-entered position requirements to be calibrated through human-computer interaction. The storage module is used to preset the default position, position calibration standard parameters, and store position calibration historical data. The calibration device 100 also includes a power supply module, a control circuit board and a communication module. In this embodiment, the analysis device controls the motion module to drive the calibration device 100 to perform position calibration, and can control the display interaction module to display the relevant operating steps and parameters of this position calibration. The analysis device, the calibration device 100 and the nucleic acid detection device 200 can be connected for communication via a cable or a WIFI module.

[0117] The analysis device can establish a communication connection with the nucleic acid detection device 200 via a wired or wireless network; taking an external computer as an example, the nucleic acid detection device 200 enters a static IP automatic setting via Ethernet, and then the calibration device 100 establishes a communication connection serial port mode with the analysis device via a USB interface, and calibration control is performed via the external computer. In another embodiment, the analysis device is integrated into one of the calibration device 100 or the nucleic acid detection device 200.

[0118] In one embodiment, the calibration input information also includes a position to be analyzed, and the analysis device is further configured to obtain at least one of the position to be calibrated and the position to be analyzed based on the position input instruction. The analysis device in this embodiment can control the nucleic acid detection device 200 to enter a custom mode. Compared to the standard mode, it can further obtain the position to be calibrated and the position to be analyzed. When professionals calibrate the nucleic acid detection device 200, they can select the position to be calibrated and the position to be analyzed according to specific requirements, making the position calibration system more compatible with different calibration requirements.

[0119] The user can select the position to be calibrated and the position to be analyzed from the Y-axis amplification position, Y-axis extraction position, Z-axis extraction position and S-axis extraction position. After the position to be calibrated and the position to be analyzed are selected, the analysis device can control the motion module to perform calibration operations, and the analysis device can detect the current position through the calibration device 100. The calibration device 100 in this embodiment can use photoelectric detection. And calculate the calibration deviation value of the position to be analyzed. The analysis device can display the calibration deviation value of the position to be analyzed, which is convenient for the user to understand the position deviation of the current nucleic acid detection device 200, which is more humane. The analysis device can send the calibration deviation value of the position to be calibrated to the nucleic acid detection device 200, and replace the default position in the nucleic acid detection device 200 with the current position.

[0120] In another embodiment, the analysis device first calculates and displays a calibration deviation value for one or more of the Y-axis amplification position, the Y-axis extraction position, the Z-axis extraction position, and the S-axis extraction position, so that the user can select the position to be analyzed and the position to be calibrated. If the calibration deviation value is small, the user can select position calibration. If the calibration deviation value is large, the user can not select position calibration. In this embodiment of the application, the analysis device is further configured to:

[0121] Start timing while performing position calibration;

[0122] If it is determined that the calibration time exceeds the preset time, the motion module is controlled to reset and a timeout alarm is issued.

[0123] The analysis device in this embodiment starts the calibration timer while controlling the motion module to drive the calibration device 100 to perform position calibration, and determines whether the calibration time exceeds the preset time. The preset time in this embodiment can be 5 minutes. If the calibration time exceeds 5 minutes, a timeout alarm is issued, and the user can obtain fault information of the position calibration system as soon as possible.

[0124] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of such features. In the description of this application, "plurality" means at least two, for example, two, three, etc., unless otherwise specifically defined.

[0125] In this application, unless otherwise specified or limited, the terms "installed," "connected," "connect," "fixed," etc. should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integration; mechanical connection, electrical connection, or communication; direct connection or indirect connection through an intermediate medium; internal communication between two elements or interaction between two elements, unless otherwise specified. Those skilled in the art will understand the specific meanings of the above terms in this application based on specific circumstances.

[0126] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0127] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A calibration device for calibrating a POCT all-in-one machine with a PCR module and a pipetting extraction module, wherein, The calibration device includes: A calibration main body for adapting to the POCT integrated machine; A first position calibration mechanism installed on the calibration main body and including a position adjustment part and a first detector. The position adjustment part can cooperate with the first detector under the push of the PCR module to detect the Y-axis coordinate of the amplification position; A temperature probe installed on the calibration main body and adapted to the PCR module to detect the heating temperature of the PCR module.

2. The calibration device according to claim 1, wherein, The first position calibration mechanism further includes a second detector for cooperating with the liquid transfer and extraction module to detect the Y-axis coordinate and Z-axis coordinate of the extraction position; And / or A limit card slot adapted to the POCT integrated machine is provided at the bottom of the calibration main body.

3. The calibration device according to claim 1, wherein, The position adjustment part includes: A sliding main body. A sliding cavity is provided on the calibration main body. The sliding main body is accommodated in the sliding cavity and can reciprocally slide between an initial position and a detection position relative to the sliding cavity; Two limit hooks are respectively arranged at both ends of the sliding main body along the Y-axis and are in limit cooperation with the calibration main body.

4. The calibration device according to claim 3, wherein, The sliding main body includes: A main body plate extending along the Y-axis; A limit ear protruding along the Z-axis from the edge of the main body plate. The sliding cavity is provided with a limit part corresponding to the limit ear, and the size of the limit part is larger than that of the limit ear.

5. The calibration device according to claim 3, wherein, An assembly port is provided in the sliding cavity. The first position calibration mechanism further includes a protective pressure plate arranged at the assembly port and detachably connected to the calibration main body. The protective pressure plate is used to protect the position adjustment part and the first detector.

6. The calibration device according to claim 1, wherein, The first position calibration mechanism further includes an elastic reset part for driving the position adjustment part to reset. The elastic reset part is connected between the calibration main body and the position adjustment part, or the elastic reset part is connected between the first detector and the position adjustment part.

7. The calibration device according to claim 1, wherein, The calibration device includes a second position calibration mechanism installed on the calibration main body and including a third detector for cooperating with the liquid transfer and extraction module to detect the rotation coordinate of the extraction position. The first position calibration mechanism further includes a second detector for cooperating with the liquid transfer and extraction module to detect the Y-axis coordinate and Z-axis coordinate of the extraction position; And / or The calibration main body is provided with a profiling base adapted to the POCT integrated machine.

8. The calibration device according to claim 1, wherein, A temperature detection cavity is provided in the calibration main body. The calibration device further includes a first temperature detector passing through the temperature detection cavity. One end of the first temperature detector is recessed in the temperature detection cavity, and the other end protrudes from the temperature detection cavity and is used to detect the heating module temperature of the POCT integrated machine.

9. The calibration device according to claim 8, wherein, The first temperature detector includes a sensor body accommodated in the temperature detection cavity and a detection telescopic head protruding from the temperature detection cavity. An elastic avoidance part is connected between the detection telescopic head and the sensor body.

10. The calibration device according to claim 8, wherein, The first temperature detector includes a heat conduction part, a temperature sensor, and a heat insulation plate. The temperature sensor is arranged in the heat conduction part, and one end of the heat conduction part penetrates through the heat insulation plate and is inserted into the calibration main body.

11. The calibration device according to claim 10, wherein, A cable cover plate is further provided on the outer periphery of the heat insulation plate, and the cable cover plate and the calibration main body cooperate to wrap the outer peripheries of the heat insulation plate and the heat conduction part.

12. A position calibration system for a nucleic acid detection device with a motion module, wherein, The position calibration system includes an analysis device and the calibration device according to claim 1. The calibration device is adapted to the nucleic acid detection device and is used to detect the motion module. The calibration device and the nucleic acid detection device are both communicatively connected to the analysis device; The analysis device is configured to: Obtain calibration input information and device information of the nucleic acid detection device; Combine the calibration input information and the device information to obtain a position to be calibrated and a calibration operation instruction; Control the motion module to drive the calibration device to move to the position to be calibrated; Start calibration and send the calibration operation instruction to the nucleic acid detection device.

13. The calibration device according to claim 12, wherein, The calibration input information includes a mode input instruction, and the analysis device is further configured to: Determine that the mode input instruction includes the standard mode, and obtain the calibration operation instruction and the position to be calibrated according to the device information; Determine that the mode input instruction includes the custom mode, and obtain the calibration operation instruction and the position to be calibrated according to the calibration input information.

14. The calibration device according to claim 13, wherein the calibration input information further includes a position input instruction, and the analysis device is further configured to: Determine that the calibration input information includes the custom mode and obtain the position input instruction; Determine a custom option according to the position input instruction, and the custom option includes at least one or more of a Y-axis amplification position, a Y-axis extraction position, a Z-axis extraction position, and an S-axis extraction position.

15. The calibration device according to claim 12, wherein, The analysis device is further configured to: Obtain the current position detection information of the calibration device for the position to be calibrated; Calculate the calibration deviation value of the position to be calibrated; Determine that the calibration deviation value is less than a preset deviation value, and mark the current position of the position to be calibrated as the calibration position.

Images