Method of combined extraction of marine collagen and chitosan

Abstract

The invention relates to a combined extraction method for obtaining a mixture of marine collagen and chitosan from fishing waste by green technology optimization.

Description

[0001] METHOD OF COMBINED EXTRACTION OF MARINE COLLAGEN AND CHITOSAN

[0002] Technical Field

[0003] The present invention relates to a method of obtaining a mixture of marine collagen and chitosan from fishing waste.

[0004] State of the Art (Background)

[0005] Marine collagen can be obtained from a variety of sources, including fish, invertebrate marine animals, and marine byproducts like skin, scales, bone, and fins. Collagen extraction methods include acidic soluble collagen (ASC) extraction and pepsin-soluble (PSC) extraction. Marine collagen is increasingly used in various fields, including pharmaceutical and biomedical devices, nutraceuticals, cosmeceuticals, food and beverages, bioplastics, and biomaterials. Marine collagen is also used for tissue regeneration and drug delivery.

[0006] Shrimp, crab, and prawn shells are the primary sources of marine-derived chitosan in industry, accounting for approximately 80% of chitin production. The process of extracting chitin from shells includes several steps, including demineralization, deproteination, bleaching / decolorization, and finally deacetylation to produce chitosan. In demineralization, the shells are washed and dried before being pulverized; in deproteination, the shell powder is treated with chemicals to remove proteins; in bleaching / decolorization, the treated shell powder is bleached to remove pigments and obtain colorless pure chitin; and in deacetylation, the chitin is treated with alkali to remove acetyl groups and obtain chitosan.

[0007] Chitosan has a wide range of applications, including the fields of biomedical, pharmaceutical, food, textile, and waste treatment.

[0008] Previous methods and systems are based on traditional methods, which are commonly used in the treatment of industrial waste. These methods are frequently insufficient to mitigate environmental impacts and ensure sustainability. In this context, the technical issues and some of the problems encountered in the prior art are listed below:

[0009] Environmental Impacts: Traditional methods are often harmful to the environment and do not ensure effective waste recycling or utilization. This poses a significant challenge to environmental sustainability.

[0010] Efficiency Issues: Previous systems have inefficient waste treatment processes. There are difficulties regarding the extraction of various valuable components from waste and making them usable.

[0011] Chemical Use: In some older methods, aggressive chemicals were commonly used to treat waste. This can harm the environment and raise energy costs.

[0012] Costs: Traditional methods are not economically feasible since they are frequently associated with high operating costs.

[0013] Brief Description and Objects of the Invention

[0014] The extraction of marine collagen and chitosan from fishing wastes, specifically fish and shellfish wastes, within the scope of the present invention has been developed to address the aforementioned issues and provide a more sustainable waste treatment process by combining an optimized method with green technology.

[0015] This new approach comprises more efficient and environmentally friendly processes for extracting valuable components from waste products.

[0016] It also lowers energy costs and promotes a more sustainable manufacturing process by reducing environmental impact.

[0017] The objects of the invention can be listed as:

[0018] -Ensuring sustainable waste management by transforming waste into valuable products, -Minimizing environmental impact by reducing energy and resource consumption. -Extracting more efficiently than previous methods,

[0019] -Providing a more reliable and effective manufacturing process by solving technical problems encountered in traditional systems,

[0020] -Producing a high-quality product that exceeds industry expectations.

[0021] The present invention aims to bring innovation to the industry by introducing significant improvements in sustainability, efficiency, and technology.

[0022] Detailed Description of the Invention

[0023] The invention comprises a method of extracting marine collagen and chitosan from fishing waste using green technology.

[0024] Marine collagen and chitosan from fish and shellfish waste are extracted using an extraction method optimized with green technology. This process provides better efficiency and quality.

[0025] The extraction process uses green technology devices and chemicals that are both environmentally friendly and sustainable. Thus, the environmental impact of waste is reduced.

[0026] The invention offers an environmentally friendly and sustainable production process based on green technology principles.

[0027] Within the scope of the invention, the use of chemicals in the waiting and pre-treatment processes, which are generally used in the production of collagen and chitosan, is minimized.

[0028] A combined extraction method for obtaining marine collagen and chitosan simultaneously and in combination from fishing waste within the scope of the invention comprises the following process steps; i. cold pressing of fishing waste, ii. washing the resulting pulp with pure water, iii. soaking the washed pulp in pure water at a predetermined temperature and time in an ultrasound extraction device, iv. washing the remaining pulp with pure water before placing it in the extractor, v. taking the pretreated pulp taken from the extractor into the supercritical CO2 extraction device and keeping it at a pressure in the range of 300-400 bar at a predetermined temperature and time, then reducing device pressure to a value within the range of 50-100 bar and kept for a predetermined period of time, then increasing it again to a pressure within the range of 300-400 bar and keeping it for a predetermined period of time, vi. obtaining the mixture of marine collagen and chitosan in liquid form after the mixture of collagen and chitosan is taken back to the extractor and kept at 50 bar pressure at a predetermined temperature and time.

[0029] Detailed steps of the method in the preferred embodiment of the invention and steps in alternative embodiments of the invention are described below.

[0030] In the pretreatment, the fishing waste is cold pressed to remove unwanted materials. The resulting pulp is then washed with pure water and then kept in pure water in an ultrasound extraction device. The remaining pulp is washed with pure water in order to remove the minerals and small protein and fat molecules to be removed and then it is taken to the extractor.

[0031] In the preferred embodiment of the invention, shrimp shells and fish skin are cold pressed together at a 1 : 1 ratio to remove oil and similar materials. The pulp is then washed 3 times with pure water and kept in an ultrasound extraction device at a temperature of 40-80 degrees for 60-90 minutes, with pure water, preferably in a 1 : 10 pure water solution, before the remaining pulp is washed 3 times with pure water and transferred to the extractor. It is preferably kept in an ultrasound extraction device at 40 degrees for 60 minutes. Again, a 1 :1 ratio is used to ensure that the target bio compounds are obtained in similar percentages and proportional amounts.

[0032] The invention includes a method of combined extraction of marine collagen and chitosan simultaneously from fish and shellfish waste. The process comprises the processes of taking the pretreated pulp into a supercritical CO2 extraction device and holding it at predetermined pressures, predetermined times, and temperatures, then returning the resulting mixture of liquid collagen and chitosan into an extractor and holding it again at predetermined pressures, predetermined times, and temperatures to obtain the final product output.

[0033] The pretreated pulp is placed in the chamber of the supercritical CO2 extraction device and held for 1-2 hours at a pressure of 300-400 bar and a temperature of 40-60 degrees, after which the device pressure is reduced to a value of 50-100 bar and held for a while before being increased to a pressure of 300-400 bar and held for a while. What is desired here is to remove the material from the tissues via mechanical impact.

[0034] In the preferred embodiment of the invention, the pretreated pulp is placed in the supercritical CO2 extraction device chamber with 1 :10 pure water. It is first kept at 320 bar and 60 degrees for 1 hour, then reduced to a value in the range of 50-100 bar for 15 minutes, and then increased to 320 bar for 2 hours. The resulting liquid collagen and chitosan mixture is then transferred to a container before being returned to the high-pressure extractor. It is kept for 15 minutes at 180 degrees and 50 bar pressure in the extractor, resulting in a liquid mixture of marine collagen and chitosan.

[0035] This mixture contains chitosan in the concentration range of 5-8% g / ml and collagen in the range of 7-10% g / ml.

[0036] In alternative embodiments of the invention, the mixture obtained can be lyophilized into powder form.

[0037] Again, in alternative embodiments of the invention, this powder material can be obtained in the form of a sponge.

[0038] Supercritical extraction, ultrasounds and cold pressing processes included in the invention's method are used in different parameters to obtain the final product without using enzymes.

[0039] Meanwhile, environmentally friendly chemicals used in the extraction process are more sustainable compared to traditional systems.

[0040] Therefore, the invention minimizes environmental impact and improves energy efficiency by emphasizing the use of green energy.

[0041] The end product of the invention is high-quality marine collagen and chitosan exceeding industrial standards.

[0042] The invention provides technical innovations, environmental sustainability, and high-quality products, enabling effective use of fishing waste. Marine collagen and chitosan, which are obtained using green technology, have a wide range of applications in the cosmetics and biomedical industries. Some potential applications in these sectors are as follows:

[0043] - In the cosmetics industry; in anti-aging products, in skin care products, in hair care products, and in make-up products.

[0044] - In the biomedical industry; wound healing creams and bandages, in tissue engineering to produce implants used in wound repair, bone regeneration and other tissue regeneration processes, in orthopedic implants and in the field of dermatology.

[0045] These applications demonstrate that the invention has a broad range of applications in the cosmetic and biomedical industries. Since using green technology and sustainable raw materials are becoming more important in these industries, the invention will be able to meet these demands.

Claims

CLAIMS1. A combined extraction method for obtaining marine collagen and chitosan simultaneously and in combination from fishing waste, characterized in that it comprises the following process steps; i. cold pressing of fishing waste, ii. washing the resulting pulp with pure water, iii. soaking the washed pulp in pure water at a predetermined temperature and time in an ultrasound extraction device, iv. washing the remaining pulp with pure water before placing it in the extractor, v. taking the pretreated pulp taken from the extractor into the supercritical CO2 extraction device and keeping it at a pressure in the range of 300-400 bar at a predetermined temperature and time, then reducing device pressure to a value within the range of 50-100 bar and keeping for a predetermined period of time, then increasing it again to a pressure within the range of 300-400 bar and keeping it for a predetermined period of time, vi. obtaining the mixture of marine collagen and chitosan in liquid form after the mixture of collagen and chitosan is taken back to the extractor and kept at 50 bar pressure at a predetermined temperature and time.

2. The method according to Claim 1, characterized in that it comprises the step of lyophilizing the mixture of marine collagen and chitosan in liquid form into powder form.

3. The method according to Claim 1, characterized in that the shrimp shells and fish skin are cold pressed together.

4. The method according to Claim 1 or 3, characterized in that the shrimp shells and the fish skin are cold pressed together in a 1 :1 ratio.

5. The method according to Claim 1, characterized in that it is kept in a pure water solution in an ultrasound extraction device at a temperature in the range of 40-80 degrees for a period of 60-90 minutes.

6. The method according to Claim 1 or 5, characterized in that it is soaked in a 1 : 10 pure water solution in an ultrasound extraction device at 40 degrees for 60 minutes.

7. The method according to Claim 1, characterized in that it is first kept in a supercritical CO2 extraction device at 320 bar pressure and 60 degrees for 1 hour, then the device pressure is reduced to a value in the range of 50-100 bar and kept for 15 minutes before being increased to 320 bar again and kept for 2 hours.

8. The method according to Claim 1, characterized in that the mixture of collagen and chitosan is kept in the extractor at 50 bar pressure, 180 degrees for 15 minutes.

9. A mixture of marine collagen and chitosan obtained by a method according to any one of the preceding claims.

10. The mixture according to Claim 9, characterized in that it comprises chitosan in a concentration of 5-8% g / ml and collagen in concentration of 7-10% g / ml.