Recombinant collagen type vii, preparation method therefor and the use thereof

WO2025185547A8PCT designated stage Publication Date: 2025-10-02JIANGSU TRAUTEC MEDICAL TECH CO LTD
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Patent Information

Application Number
PCT/CN2025/080137
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-03-05
Filing Date
2025-03-03
Publication Date
2025-10-02

AI Technical Summary

Technical Problem

In the existing technology, the expression level of type VII collagen is low and its stability is poor, making it difficult to achieve large-scale production. In addition, the existing expression system is expensive and the purification steps are complicated, making it impossible to industrialize.

Method used

The eukaryotic Pichia pastoris expression system was used to construct a recombinant vector to efficiently express type VII collagen in Pichia pastoris. Yeast's own enzyme cleavage technology was used to avoid in vitro enzyme cleavage, increase expression levels, and simplify the purification process.

Benefits of technology

The efficient secretory expression of type VII collagen was achieved, and the difficulty of purification was reduced. The obtained recombinant protein is similar to the natural protein, has good biological functions and tissue absorption effects, and is suitable for use in the fields of drugs, medical devices, biomaterials, etc.

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Abstract

Provided are a recombinant collagen type VII, a preparation method therefor and the use thereof, belonging to the fields of biology, genetic engineering and biologic technologies. Genetic engineering technology is used as a means for increasing the expression level of the recombinant collagen type VII, and an eukaryotic pichia pastoris expression system is used for performing the expression production of the recombinant collagen type VII; the recombinant collagen type VII is expressed in all pichia pastoris strains, can be efficiently secreted and expressed to the outside of cells, and is not prone to degradation during the purification stage, thus reducing the difficulty in purification. The recombinant collagen type VII has physicochemical properties and biological functions similar as that of an original sequence, has a relatively small molecular weight and achieves good absorption effect on tissue, and can be processed into collagen-containing compositions, and therefore can be applied well in each of the fields of drugs, pharmaceutical compositions, medical instruments, biological materials, tissue engineering products, cosmetics or health care products, etc.
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