A magnetization block for use in nucleic acid isolation

Abstract

The invention relates to a magnetization block (100) comprising a base (10) to be used in nucleic acid isolation, at least one cartridge (30) associated with said base (10), at least one well (31) contained in said cartridge (30) and a pretreated sample in said well (31). Its innovation is that it comprises at least one second magnet (13) that allows it to pass through a certain part of the well (31) by being connected to the base (10) in order to separate the multiple magnetic probes (1) in the sample, and the second magnet (13) is associated with the well (31) in order to ensure that the magnetic probe (1) in the sample in the well (31) is separated by the effect of the magnetic field, in a predetermined alignment, close to the well bottom (32), and partially forming a ring on the inner wall.

Description

[0001] DESCRIPTION

[0002] A MAGNETIZATION BLOCK FOR USE IN NUCLEIC ACID ISOLATION

[0003] Technical Field

[0004] The invention relates to a magnetization block comprising a base for use in nucleic acid isolation, at least one cartridge associated with said base, at least one well contained in said cartridge, and a pretreated sample in said well.

[0005] State of the Art

[0006] Today, various measuring and analysis devices are used to apply various processes such as measuring, mixing and magnetizing reactive materials in laboratories. The placement of reactive materials or mixtures into the measurement and analysis devices or removal of them is carried out by means of carrier apparatus called reagent cartridges.

[0007] In the state of the art / present applications, during the separation of nucleic acid from high-volume samples during the isolation process, point magnetization is applied at one or more points in the working reservoirs and the magnetizable materials in the liquid are collected at these points. When this collection is performed at one point, not all magnetizable products in the liquid can be collected in the desired amount, and the product accumulation formed at this collection point cannot be dissolved and dispersed by the liquid in subsequent processes.

[0008] In another state of the art / present application, existing systems generally magnetize the reservoir from 1 , 2 or 4 points to reduce this accumulation and increase collection performance. While increasing the magnetization points improves the process, it becomes impossible to increase this number after a point.

[0009] As a result of the search on the subject, the application numbered EP0885958, titled as Method for processing biopolymers, microorganisms or materials using more than one type of magnetic particles is found. In the relevant application, the method of processing biopolymers, microorganisms and substances using two or more kinds of magnetic particles can automatically and consistently perform a series of operations such as extraction, isolation or measurement of DNA and the like by using at least two or more kinds of magnetic particles together and can also completely prevent cross contamination. The processing method of this invention is carried out as follows. Biopolymers, microorganisms, or substances are attached to magnetic particles using a pipette end removably mounted on the front end of the pipette head of a transfer pipette to perform a purification process, such as capturing cells to extract DNA and the like, or solubilizing cell nuclei or protein. However, in high density samples, said invention cannot fully collect and therefore isolate the genetic material.

[0010] As a result, it has become necessary to make a development in the relevant technical field due to the negativities described above and the inadequacy of existing solutions on the subject.

[0011] Object of the Invention

[0012] The invention is inspired by current situations and aims to solve the above-mentioned deficiencies.

[0013] The main object of the invention is that it comprises at least one second magnet that allows it to pass through a certain part of the well by being connected to the base, in order to separate the multiple magnetic probes provided in the sample, and the second magnet is associated with the well in order to ensure that the magnetic probe in the sample in the well is separated by the effect of the magnetic field, in a predetermined alignment near the bottom of the well, and partially forming a ring on the inner wall. Thus, the magnetic probe is separated from the sample provided in the well in a predetermined area.

[0014] Another object of the invention is to provide the second magnet essentially in the form of a ring or a C geometric form. Thus, the well is ensured to pass through the second magnet.

[0015] Another object of the invention is to comprise a first magnet provided in order to bring the second magnet closer to the well and strengthen its magnetic effect. Thus, magnetic probes are collected in an area partially distant from the bottom of the well, and the probes in the entire sample area are separated equally.

[0016] Another object of the invention is to comprise the positioning of the second magnet by the magnetic effect of the first magnet provided in more than one number. Thus, the second magnet is detachably connected at the end of the first magnet. Another object of the invention is to associate the second magnet with the well in a slot provided in at least one holder. Thus, the sample provided in the well is protected from possible external factors.

[0017] The structural and characteristic features and all the advantages of the invention will be more clearly understood by means of the drawings given below and the detailed description written with references to these drawings, and therefore the evaluation needs to be made by taking these drawings and the detailed description into consideration.

[0018] Figures to Help Understand the Invention

[0019] Figure 1 is an exploded view of the magnetization block, which is the subject of the invention, associated with a cartridge.

[0020] Figure 2 is an exploded view of the magnetization block, which is the subject of the invention.

[0021] Figure 3 is a cross-sectional view of the magnetization block, which is the subject of the invention, associated with the cartridge.

[0022] Figure 4 is an exploded view of the magnetization block, which is the subject of the invention.

[0023] Figure 5 is an exploded view of the magnetization block, which is the subject of the invention.

[0024] Figure 6 is an isometric view of the situation where the probes are collected in a magnetization block, which is the subject of the invention.

[0025] Description of Part References

[0026] 100 Magnetization Block

[0027] 1 Magnetic Probe

[0028] 10 Base

[0029] 11 Hole

[0030] 12 First Magnet 13 Second Magnet

[0031] 20 Holder

[0032] 21 Slot

[0033] 22 Notch

[0034] 23 Rabbet

[0035] 30 Cartridge

[0036] 31 Well

[0037] 32 Well bottom

[0038] 33 Stage

[0039] Detailed Description of the Invention

[0040] In this detailed description, preferred embodiments of a magnetization block (100), which is the subject of the invention are described only for a better understanding of the subject.

[0041] In Figure 1 , it is given an exploded view of the magnetization block (100), which is the subject of the invention, associated with a cartridge (30). Accordingly, the magnetization block (100) is a mechanism that enables the collection of products with the desired magnetic effect with the help of a magnetic field to be used in nucleic acid isolation. The magnetization block (100) comprises at least one base (10). Said base (10) is a bottom layer made of aluminum material. In a possible embodiment of the invention, the base (100) can be manufactured in a hard plastic, composite, steel-like structure. It comprises multiple holes (11) provided on the base (100). The magnetization block (100) comprises at least one holder (20), which is essentially a cover. The magnetization block (100) is associated with at least one cartridge (30). Said cartridge (30) is a deep well plate and is a structure containing more than one compartment containing the samples.

[0042] In Figure 2, it is given an exploded view of the magnetization block (100), which is the subject of the invention. Accordingly, the magnetization block (100) comprises at least one second magnet (13) with magnetic properties. The second magnet (13) is connected to the base (10), allowing it to pass through a certain part of the well. The magnetization block (100) contains at least one first magnet (12) with magnetic properties. The first magnet (12) is connected to the holes (11 ) provided on the base. In a possible embodiment of the invention, the first magnet (12) is provided in 4 cylindrical forms. In another possible embodiment of the invention, the first magnet (12) can take place under the condition that it can be provided in different geometric forms and in different quantities. The second magnet (13) is associated with the other end of the first magnet (12). In a possible embodiment of the invention, the second magnet (13) is associated at the end of the first magnet (12), which is provided in 4 pieces, in a way that it finds its position under the influence of the magnetic field. In this way, the second magnet (13) is fixed and supported by the magnetic effect of the first magnet (12). The first magnet (12) and the second magnet (13) are essentially preferred as neodymium magnets (NdFeB).

[0043] The holder (20) is connected to the base (10). The holder (20) comprises more than one provided slot (21). Said slots (21) are essentially provided concentrically with the second magnet (13). The holder (20) comprises at least one rabbet (23) provided in the slot (21 ). The second magnet (13) provided in the slot (21) is in contact with the rabbet (23). Said rabbet (23) prevents the second magnet (13) from being removed by external influences while helping to keep its position constant. The holder (20) comprises at least one notch (22) provided on at least one side. Said notch (22) is essentially a channel provided in the vertical direction.

[0044] The cartridge (30) comprises at least one stage (33) provided inside of its lateral walls. The cartridge (30) is connected to the holder (20) by associating the stage (33) with the notch (22). In this way, it is supported that the cartridge (30) comprises the sample in a stable manner.

[0045] The cartridge (30) connected to the magnetization block (100) comprises at least one well (31 ). Said well (31 ) is a reservoir comprising the pretreated sample. The treated sample comprises a magnetic probe (1 ). The magnetic probe (1) is microscopic particles encapsulated in a polymer matrix of a magnetic material, usually iron oxide. These beads have the ability to be manipulated using external magnetic fields. The primary use of magnetic probes (1 ) is in the extraction and purification of biological molecules such as DNA, RNA, proteins, and cells. This process is known as magnetic bead separation or magnetic bead-based purification and is accomplished by binding specific ligands or binding molecules to the surface of the beads. These ligands can selectively bind to molecules of interest.

[0046] The well (31) passes through the slot (21) provided in the holder (20) and is connected to the second magnet (13) at a predetermined position. Thus, the magnetic probe (1) in the sample in the well (31) is separated by the effect of the magnetic field, in a predetermined alignment near the bottom of the well (32), and partially forming a ring on the inner wall. In this way, the magnetic probes (1) will be collected at a certain level from the bottom of the well (32), forming a ring on the wall.

[0047] With all this structuring, magnetic probes (1) collected at the bottom (32) or in a certain region of the well, relatively ring-shaped and not collected at the bottom, are separated from the sample faster. Additionally, since it is a separation method that spreads over the surface, aggregation is prevented. In this way, it is possible to obtain more stable results from the sample. The support of the first magnet (12) is strengthened, and with the effect of the second magnet (13) provided in the form of a ring, the separation time of the magnetic probes (1) from the pretreated sample is shortened and the separation percentage increases. Stable results are obtained.

Claims

CLAIMS1. A magnetization block (100) comprising a base (10) to be used in nucleic acid isolation, at least one cartridge (30) associated with said base (10), at least one well (31 ) contained in said cartridge (30) and a pretreated sample in said well (31), characterized in that the magnetization block (100) comprises at least one second magnet (13) that allows to pass through a certain part of the well (31) by being connected to the base (10), the second magnet (13) is associated with the well (31) in order to ensure that the magnetic probe (1) in the sample in the well (31) is separated by the effect of the magnetic field, in a predetermined alignment, close to the well bottom (32), and partially forming a ring on the inner wall, wherein the separation of multiple provided magnetic probes (1 ) in the sample is ensured.

2. The magnetization block (100) according to claim 1 , characterized by comprising the second magnet (13) provided essentially in the form of a ring or a C geometric form.

3. The magnetization block (100) according to claim 1 , characterized by comprising a first magnet (12) provided in order to bring the second magnet (13) closer to the well (31 ) and strengthen magnetic effect.

4. The magnetization block (100) according to claim 1 , characterized by comprising positioning the second magnet under the magnetic effect of the first magnet provided in more than one.

5. The magnetization block (100) according to claim 1 , characterized by comprising associating the second magnet with the well in a slot provided in at least one holder.

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